JP2017044971A - Microscope system, microscope system control method, and microscope system control program - Google Patents

Abstract

To provide a microscope system, a microscope system control method, and a microscope system control program that achieve both noise reduction due to stray light, which is a problem in detection of weak light, and operability of a microscope in a dark room. A microscope system 100 according to the present invention is a microscope system for observing a specimen image formed by imaging the light of a specimen SP, and includes a display unit 10 having a backlight 11 in which a wavelength band of emitted light is variable. And a control unit 8 that outputs light of a wavelength component other than the wavelength band of the light to be observed emitted from the sample SP from the backlight 11 of the display unit 10 based on information on the sample SP. [Selection] Figure 1

Description

  The present invention relates to a microscope system for irradiating a specimen with illumination light and observing a specimen image formed based on light from the specimen, a control method for the microscope system, and a control program for the microscope system.

  2. Description of the Related Art A microscope having a function of measuring weak light such as fluorescence and capturing an image showing a distribution of light emission positions in a dark room is used. In the darkroom, a terminal device and a display for confirming a captured image and data processing are installed. However, in a dark room, light from the display enters the objective lens as stray light, and weak light cannot be observed well.

  A technique has been proposed in which light from the display is reduced by applying a mask to the background area of the image display area and other background areas on the display (see, for example, Patent Document 1). As a result, stray light from the display, which has been a problem in microscope observation for detecting weak light such as fluorescence observation, is reduced.

JP 2009-251454 A

  However, the technique described in Patent Document 1 cannot completely prevent stray light from the display because the light from the image display area is not masked. In the background area, since the amount of information that can be displayed is limited compared to the normal state, it is difficult for the operator to check the state of the microscope, and the operability of the microscope is reduced.

  The present invention has been made in view of the above, and is a microscope system that achieves both noise reduction due to stray light, which is a problem in detection of weak light, and operability of the microscope in a dark room, and control of the microscope system It is an object to provide a method and a control program for a microscope system.

  In order to solve the above-described problems and achieve the object, a microscope system according to the present invention is a microscope system for observing a specimen image formed by imaging light from a specimen, and the wavelength band of emitted light is variable. A light emitting unit; and a control unit configured to output light having a wavelength component other than a wavelength band of light to be observed emitted from the sample from the light emitting unit based on information on the sample.

  Further, in the microscope system according to the present invention, the light emitting unit is a backlight of a display unit, and the control unit uses light of a wavelength component other than a wavelength band of light to be observed emitted by the specimen to the backlight. It emits light.

  Further, in the microscope system according to the present invention, the light emitting unit is a display unit, and the control unit designs a display image using light having a wavelength component other than the wavelength band of light to be observed emitted by the sample. A design generation unit is provided, and the control unit causes the display unit to display a display image of the design generated by the design generation unit.

  In the microscope system according to the present invention, the light emitting unit includes a white light source, a plurality of filters that transmit wavelengths in different wavelength bands, and the plurality of filters movably mounted, and the white light emitted from the white light source A filter switching device that arranges any one of the plurality of filters on an optical path of light, and the control unit has, on the filter switching device, an optical path of white light emitted by the white light source, A filter that transmits light having a wavelength component other than the wavelength band of light to be observed emitted from the specimen is disposed.

  Further, in the microscope system according to the present invention, the light emitting unit is a full color LED, and the control unit causes the full color LED to emit light having a wavelength component other than the wavelength band of the observation target light emitted from the sample. It is characterized by.

  In addition, the microscope system according to the present invention reflects an epi-illumination light source unit that irradiates light to the specimen, and reflects excitation light in a specific wavelength band among the light from the epi-illumination light source part when the specimen is located in the optical path. A mirror unit that transmits only the fluorescence from the specimen, and a plurality of mirror units corresponding to fluorescence in different wavelength bands, a mirror turret capable of arranging one mirror unit on an optical path, and an optical path A detecting unit that detects the mirror unit positioned, and the control unit emits light having a wavelength component other than a fluorescent wavelength band corresponding to the mirror unit positioned in the optical path detected by the detecting unit. It is made to output from.

  Further, the microscope system according to the present invention includes the identification information of the mirror unit, the wavelength band of the fluorescence transmitted by the mirror unit, and the wavelength of the light output from the light emitting unit among the wavelength components other than the wavelength band of the fluorescence. And a storage unit that stores the components in association with each mirror unit, the control unit from the storage unit, from the light emitting unit corresponding to the mirror unit located in the optical path detected by the detection unit A wavelength component of light to be output is read out, and light in the read wavelength band is output from the light emitting unit.

  Further, a control method for a microscope system according to the present invention is a control method for a microscope system for observing a specimen image formed based on light from a specimen, and is based on information on the specimen. The light emitting unit having a variable wavelength band includes a control process of outputting light having a wavelength component other than the wavelength band of the light to be observed emitted from the specimen.

  Further, the microscope system control program according to the present invention provides a microscope system for observing a specimen image formed on the basis of light from the specimen, with respect to the light emitting unit whose wavelength band of emitted light is variable. On the basis of the information relating to the above, a control procedure for outputting light having a wavelength component other than the wavelength band of the light to be observed emitted from the specimen is executed.

  According to the present invention, based on information related to the specimen, the light component having a wavelength component other than the wavelength band of the light to be observed emitted from the specimen is output from the light emitting unit, thereby causing stray light that is a problem in detecting weak light. Noise reduction and operability of the microscope in the dark room can be achieved at the same time.

FIG. 1 is a diagram illustrating a schematic configuration of a microscope system according to the first embodiment of the present invention. FIG. 2 is a diagram illustrating an example of a table stored in the storage unit illustrated in FIG. FIG. 3 is a diagram illustrating a processing procedure of a control operation for the backlight color of the display unit of the microscope system of FIG. FIG. 4 is a diagram illustrating a schematic configuration of a microscope system according to the second embodiment. FIG. 5 is a diagram showing a processing procedure of a control operation for the design of the display image of the microscope system of FIG. FIG. 6 is a diagram illustrating a schematic configuration of the microscope system according to the third embodiment. FIG. 7 is a diagram showing a processing procedure of a control operation for the design of the display image of the microscope system of FIG. FIG. 8 is a diagram illustrating a schematic configuration of a microscope system according to a modification of the third embodiment.

  DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments for carrying out the present invention (hereinafter referred to as “embodiments”) will be described with reference to the drawings. The present invention is not limited to the embodiments described below. The drawings referred to in the following description only schematically show the shape, size, and positional relationship so that the contents of the present invention can be understood. That is, the present invention is not limited only to the shape, size, and positional relationship illustrated in each drawing.

(Embodiment 1)
FIG. 1 is a diagram illustrating a schematic configuration of a microscope system according to the first embodiment of the present invention. A microscope system 100 includes a microscope main body 1, a stage 1a on which a specimen SP that emits fluorescence when irradiated with excitation light, a light source section 2 that irradiates the specimen SP with light, and a mirror turret 3 that includes a plurality of mirror units 3a. , An objective lens 4a disposed opposite to the stage 1a, a mirror 4b for guiding fluorescence emitted from the specimen SP to the imaging apparatus 5, an imaging apparatus 5 for imaging fluorescence emitted from the specimen SP, a mirror unit position detection apparatus 6, and a microscope A controller 7 that controls driving of the system 100 and an image corresponding to image data generated by the imaging device 5 and a display unit 10 (light emitting unit) that displays a setting state of illumination light are provided. Note that the microscope system 1 includes an operation unit (not shown), and receives input of instruction information such as setting of illumination light irradiated on the specimen SP.

  The light source unit 2 includes an incident light source 2a that irradiates light and a condenser lens 2b that condenses light emitted from the incident light source 2a. The light emitted from the light source unit 2 is guided to the surface of the mirror unit 3a located on the optical path L by an optical system (not shown).

  The mirror turret 3 is equipped with a plurality of mirror units 3a corresponding to fluorescence in different wavelength bands. The mirror unit 3a is provided with a filter that transmits only light in a predetermined wavelength band. The mirror unit 3a reflects the light in the first wavelength band when light in the first wavelength band is incident, and the second light in the second wavelength band when light in the second wavelength band is incident. Transmits light in the wavelength band. When the mirror unit 3a is positioned on the optical path L, the light of the first wavelength band out of the light from the incident light source 2a is reflected to the sample SP, and only the light of the second wavelength band emitted by the sample SP is transmitted. . The first wavelength band is excitation light of the specimen SP, and the second wavelength band is fluorescence emitted by the specimen SP. Each mirror unit 3a corresponds to fluorescence in a different wavelength band. The mirror unit 3a located in the optical path L reflects the excitation light of the sample SP to the sample SP. Fluorescence emitted from the specimen SP by the irradiation of the excitation light is transmitted to the mirror unit 3a disposed in the optical path L and reflected by the mirror 4b, thereby being guided to the imaging device 5.

  The mirror turret 3 can be rotated by a motor or the like. By rotating, the position of each mounted mirror unit 3a can be changed, and one mirror unit 3a can be arranged on the optical path L. The mirror turret 3 replaces the mirror unit 3a located in the optical path L with a mirror unit 3a corresponding to the fluorescence wavelength band to be observed.

  The mirror unit position detection device 6 detects the mirror unit 3 a located in the optical path L by detecting the displacement of the mirror turret 3 in the rotation direction, and outputs the detection result to the controller 7.

  The controller 7 includes a control unit 8 and a storage unit 9. The control unit 8 controls driving of the microscope system 100. The control unit 8 causes the display unit 10 to be described later to output light having a wavelength component other than the wavelength band of the fluorescence to be observed emitted by the sample SP based on the information about the sample SP. The control unit 8 causes the display unit 10 to output light having a wavelength component other than the fluorescence wavelength band corresponding to the mirror unit 3 a located in the optical path L detected by the mirror unit position detection device 6.

  The storage unit 9 stores an execution program for the control unit 8 to execute various operations, a control program, and various types of information regarding the operation of the microscope system 100. FIG. 2 is an example of a table stored in the storage unit 9. As in the table T1 illustrated in FIG. 2, the backlight of the display unit 10 to be described later among the identification information of the mirror unit 3a, the wavelength band of fluorescence corresponding to the mirror unit 3a, and wavelength components other than the wavelength band of fluorescence The wavelength λ of the light output from 11 (light emitting unit) is associated with each mirror unit 3a. Based on the detection result of the mirror unit position detection device 6, the control unit 8 reads the wavelength λ of the output light from the display unit 10 corresponding to the mirror unit 3a located in the optical path L from the table T1 of the storage unit 9, The read light of wavelength λ is output from the backlight 11. Specifically, the control unit 8 transmits a backlight color change command so that light of the wavelength λ corresponding to the mirror unit 3 a located in the optical path L is output from the backlight 11.

  The display unit 10 includes a backlight 11 having a variable wavelength band of emitted light, and includes a backlight control unit 12 that controls the light emission operation of the backlight. The backlight control unit 12 changes the emission color of the backlight 11 so as to correspond to the light of the wavelength λ according to the backlight color change command from the control unit 8.

  Next, a control operation for the backlight color of the display unit 10 of the microscope system 100 will be described. FIG. 3 is a diagram illustrating a processing procedure of a control operation for the backlight color of the display unit of the microscope system 100. When the fluorescence observation is started, the control unit 8 inquires the mirror unit position detection device 6 which mirror unit 3a is currently located in the optical path L, and determines the type of the mirror unit 3a currently located in the optical path L. The mirror unit position information shown is acquired (step S1).

  The control unit 8 refers to the table T1 of the storage unit 9 based on the mirror unit position information acquired in step S1, and sets the wavelength λ of the output light corresponding to the type of the mirror unit currently located in the optical path L as the output light information. (Step S2).

  The control unit 8 transmits a command for changing the emission color of the backlight 11 to the backlight control unit 12 so that light of wavelength λ is output from the backlight 11, and the emission color of the backlight 11 of the display unit 10. Is changed (step S3). As a result, light of a wavelength component other than the wavelength band of the fluorescence to be observed emitted from the specimen SP is emitted from the backlight 11.

  The control unit 8 determines whether or not the end of the fluorescence observation is instructed based on the instruction information from the operation unit (not shown) (step S4). If the control unit 8 determines that the end of fluorescence observation is not instructed (step S4: No), the control unit 8 communicates with the mirror unit position detection device 6 to change the mirror unit 3a located in the optical path L. It is determined whether or not (step S5). When the control unit 8 determines that the mirror unit 3a located in the optical path L has been changed (step S5: Yes), the control unit 8 returns to step S1 and applies the backlight color to the mirror unit 3a newly located in the optical path L. Repeat the process related to the change. If the control unit 8 determines that the mirror unit 3a located in the optical path L has not been changed (step S5: No), the control unit 8 returns to step S4 and determines the end of fluorescence.

  If the control unit 8 determines that the end of fluorescence observation has been instructed (step S4: Yes), the control unit 8 causes the backlight control unit 12 to return the backlight 11 of the display unit 10 to the original color. An instruction to change the emission color of the light 11 is transmitted. Thereby, the backlight control unit 12 returns the emission color of the backlight 11 to the original color (step S6), and the fluorescence observation is ended.

  As described above, according to the first embodiment, the light emission color of the backlight 11 of the display unit 10 is automatically changed according to the observation state, and the light of the wavelength component other than the wavelength band of the fluorescence to be observed emitted by the specimen SP. Change to a color corresponding to. For this reason, according to Embodiment 1, since the light output from the backlight 11 of the display unit 10 does not pass through the mirror unit 3a, it does not become stray light, and the captured image in the imaging device 5 is displayed. Also has no effect. Further, according to the first embodiment, since only the emission color of the backlight 11 of the display unit 10 is changed, the amount of information that can be displayed is not reduced, and the state of the microscope and the operation of the microscope by the operator are reduced. Is also fully feasible.

(Embodiment 2)
Next, a second embodiment will be described. FIG. 4 is a diagram showing a schematic configuration of a microscope system according to Embodiment 2 of the present invention. As shown in FIG. 4, the microscope system 200 according to the second embodiment has a controller 207 having a control unit 208 and a display image design creation unit 209, a liquid crystal display unit 211, and a display control unit, as compared with the microscope system 100. And a display section 210 (light emitting section) having 212.

  Based on the detection result of the mirror unit position detection device 6, the control unit 208 reads the wavelength λ of the output light from the display unit 210 corresponding to the mirror unit 3a located in the optical path L from the table T1 of the storage unit 9, Output light information indicating the wavelength λ of the read output light is output to the display image design creation unit 209.

  Based on the output light information, the display image design creation unit 209 changes the design of the display image displayed on the display unit 210 to a display image using light having a wavelength λ corresponding to the mirror unit 3a located in the optical path L. The design is changed to a design, and a display image design change command is output to the display unit 210.

  In the display unit 210, the display control unit 212 causes the liquid crystal display unit 211 to display a display image of the changed design according to the change command input from the display image design creation unit 209.

  Next, the control operation for the design of the display image of the display unit 210 of the microscope system 200 will be described. FIG. 5 is a diagram illustrating a processing procedure of a control operation for the design of a display image of the microscope system 200. Steps S11 and S12 shown in FIG. 5 are steps S1 and S2 shown in FIG. Based on the output light information input from the control unit 208, the display image design creation unit 209 changes the design of the display image displayed on the display unit 210 to light having a wavelength λ corresponding to the mirror unit 3a located in the optical path L. (Step S13), and a display image design change command is output to the display unit 210. In accordance with the display image design change command, in the display unit 210, the display control unit 212 causes the liquid crystal display unit 211 to display the display image of the changed design. Steps S14 and S15 are steps S4 and S5 shown in FIG. If the control unit 208 determines that the end of fluorescence observation has been instructed (step S14: Yes), the control unit 208 transmits a command to the display control unit 212 to return the display image design of the display unit 210 to the original design. To do. As a result, the display control unit 212 returns to the design of the display image on the liquid crystal display unit 211 (step S16) and ends the fluorescence observation.

  Even if the backlight color cannot be changed due to hardware restrictions as in the second embodiment, the design of the display image of the display unit 210 is set to a wavelength other than the wavelength band of the fluorescence to be observed emitted by the specimen SP. By performing software processing for changing to a design that uses a color corresponding to the light of the component, it is possible to achieve the same effect as in the first embodiment.

(Embodiment 3)
Next, Embodiment 3 will be described. FIG. 6 is a diagram illustrating a schematic configuration of a microscope system according to the third embodiment of the present invention.

  As shown in FIG. 6, the microscope system 300 according to the third embodiment is different from the microscope system 100 in that the light source is a white light source 314 as a working lamp in an environment where fluorescence observation is performed, A filter switching device 316 equipped with a plurality of filters 315 that transmit light, and a filter switching control unit 317 that performs switching control of the filter switching device 316 are provided. The controller 307 includes a control unit 308 that controls driving of the microscope system 300.

  The filter 315 corresponds to each mirror unit 3a, and transmits only light having a predetermined wavelength component other than the fluorescence wavelength band transmitted by the corresponding mirror unit 3a. The filter 315 blocks light having a wavelength other than the output light wavelength λ shown in the table T1 according to the type of the corresponding mirror unit 3a.

  The filter switching device 316 has a plurality of filters 315 movably mounted thereon, and arranges any one of the plurality of filters 315 on the optical path of white light emitted from the white light source 314. The filter switching device can be rotated by, for example, a motor, and the position of the mounted filter 315 can be changed by rotating. The filter switching control unit 317 controls switching of the filter 315 disposed on the optical path of white light emitted from the white light source 314 by controlling the rotation operation of the filter switching device 316.

  The control unit 308 transmits light of a wavelength component other than the wavelength band of the fluorescence to be observed emitted by the sample SP on the optical path of white light emitted by the white light source 314 to the filter switching device 316 via the filter switching control unit 317. A filter 315 that transmits light is disposed.

  FIG. 7 is a diagram illustrating a processing procedure of a control operation for the design of a display image of the microscope system 300. Steps S21 and S22 shown in FIG. 7 are steps S1 and S2 shown in FIG. That is, the control unit 308 reads the wavelength λ of the output light from the light emitting unit corresponding to the mirror unit 3a located in the optical path L from the table T1 of the storage unit 9 based on the detection result of the mirror unit position detection device 6. . The control unit 308 outputs to the filter switching control unit 317 a switching command for switching the filter 315 located in the optical path of white light emitted from the white light source 314 to the filter 315 that transmits the wavelength λ of the read output light. As a result, the filter switching control unit 317 controls the filter switching operation of the filter switching device 316, and causes the filter 315 located on the optical path of white light to have a wavelength component other than the wavelength band of the fluorescence to be observed emitted by the sample SP. The filter is switched to the filter 315 that transmits light (step S23). Steps S24 and S25 are steps S4 and S5 shown in FIG. If the control unit 308 determines that the end of fluorescence observation has been instructed (step S24: Yes), the control unit 308 issues an instruction to the filter switching control unit 317 to remove the filter 315 from the white light path of the white light source 314. Send. Accordingly, the filter switching control unit 317 controls the filter switching device 316, removes the filter 315 from the white light path of the white light source 314 (step S26), and ends the fluorescence observation.

  In the third embodiment, the observation environment can be automatically illuminated with light of a wavelength component other than the wavelength band of the fluorescence of the observation target emitted from the specimen SP according to the observation situation. Therefore, the operability in observation can be further improved.

(Modification of Embodiment 3)
FIG. 8 is a diagram illustrating a schematic configuration of a microscope system according to a modification of the third embodiment. As shown in FIG. 8, the microscope system 400 according to the modification of the third embodiment controls the full-color LED 418 and the full-color LED 418 in which the wavelength band of emitted light is variable as a light emitting unit, as compared with the microscope system 300. A full color LED control unit 419 is provided. The controller 407 includes a control unit 408 having the same function as the control unit 308. The control unit 408 causes the full-color LED 418 to emit light having a wavelength component other than the wavelength band of the fluorescence to be observed emitted from the specimen SP via the full-color LED control unit 419. The control unit 408 reads out the wavelength λ of the output light of the full color LED 418 corresponding to the mirror unit 3 a located in the optical path L from the table T 1 of the storage unit 9 based on the detection result of the mirror unit position detection device 6. The control unit 408 outputs a light emission command for emitting the light having the read wavelength λ to the full color LED control unit 419. Accordingly, the full color LED control unit 419 causes the full color LED 418 to emit light having a wavelength λ that is a wavelength other than the wavelength band of the fluorescence to be observed emitted from the specimen SP.

  As in the modification of the third embodiment, a full-color LED 418 is used instead of a white light source, and light having a wavelength λ other than the wavelength band of the fluorescence to be observed emitted by the specimen SP is emitted according to the command of the control unit 408. However, the same effects as those of the third embodiment are obtained.

  Note that the control units 8, 208, 308, and 408 emit light colors such as lamps indicating the switching state of switches provided in the microscope main body 1 and panel lamps of the controllers 7, 207, 307, and 407 during fluorescence observation. You may change into the color corresponding to the light of wavelength components other than the wavelength band of the fluorescence of the observation object which the sample SP emits. Further, the control units 8, 208, 308, and 408 may perform control so that the light amount of each light emitting unit is lower than the light amount in the case other than the time of fluorescence observation during the fluorescence observation. Further, the microscope systems 100, 200, 300, and 400 are not limited to fluorescence, and may be for observing light emitted from the specimen SP.

  A program for each process executed by the components of the microscope systems 100, 200, 300, and 400 according to the first to third embodiments is an installable format or an executable format file such as a CD-ROM, a flexible disk, It may be configured to be recorded on a computer-readable recording medium such as a CD-R or DVD and provided by being stored on a computer connected to a network such as the Internet and downloaded via the network. You may comprise. Further, it may be configured to be provided or distributed via a network such as the Internet.

DESCRIPTION OF SYMBOLS 1 Microscope main body 1a Stage 2 Light source part 2a Incident light source 2b Condensing lens 3 Mirror turret 3a Mirror unit 4a Objective lens 4b Mirror 5 Imaging device 6 Mirror unit position detection device 7,207,307,407 Controller 8,208,308, 408 Control unit 9 Storage unit 10,210 Display unit 11 Backlight 12 Backlight control unit 100, 200, 300, 400 Microscope system 209 Display image design creation unit 211 Liquid crystal display unit 212 Display control unit 314 White light source 315 Filter 316 Filter switching Device 317 Filter switching control unit 418 Full color LED
419 Full color LED controller

Claims (9)

A microscope system for observing a specimen image formed by imaging light from a specimen,
A light-emitting unit having a variable wavelength band of emitted light;
Based on information on the sample, a control unit that outputs light of a wavelength component other than the wavelength band of light to be observed emitted by the sample from the light emitting unit,
A microscope system comprising: The light emitting unit is a backlight of a display unit,
The microscope system according to claim 1, wherein the control unit causes the backlight to emit light having a wavelength component other than a wavelength band of light to be observed emitted from the specimen. The light emitting unit is a display unit,
The control unit includes a design generation unit that generates a design of a display image using light of a wavelength component other than the wavelength band of light to be observed emitted by the specimen,
The microscope system according to claim 1, wherein the control unit causes the display unit to display a display image of the design generated by the design generation unit. The light emitting unit
A white light source,
A plurality of filters that transmit wavelengths in different wavelength bands;
A filter switching device that movably mounts the plurality of filters, and that arranges any one of the plurality of filters on an optical path of white light emitted from the white light source;
Have
The control unit causes the filter switching device to dispose a filter that transmits light of a wavelength component other than a wavelength band of light to be observed emitted by the sample on an optical path of white light emitted by the white light source. The microscope system according to claim 1, characterized in that: The light emitting unit is a full color LED,
The microscope system according to claim 1, wherein the control unit causes the full-color LED to emit light having a wavelength component other than a wavelength band of light to be observed emitted from the specimen. An epi-illumination light source for irradiating the specimen with light;
A mirror unit that reflects excitation light of a specific wavelength band of light from the epi-illumination light source unit when located in the optical path to the specimen and transmits only fluorescence from the specimen;
A plurality of the mirror units corresponding to fluorescence of different wavelength bands are mounted, and a mirror turret capable of arranging one mirror unit on an optical path;
A detection unit for detecting the mirror unit located in the optical path;
With
The said control part outputs the light of wavelength components other than the fluorescence wavelength band according to the mirror unit located in the said optical path which the said detection part detected from the said light emission part, The any one of Claims 1-5 characterized by the above-mentioned. The microscope system according to any one of the above. The mirror unit identification information, the wavelength band of the fluorescence transmitted by the mirror unit, and the wavelength component of the light output from the light emitting unit among the wavelength components other than the wavelength band of the fluorescence correspond to each mirror unit. A storage unit for storing the information;
The control unit reads, from the storage unit, a wavelength component of light output from the light emitting unit corresponding to a mirror unit located in the optical path detected by the detection unit, and outputs light in the read wavelength band to the light emitting unit The microscope system according to claim 6, wherein the microscope system outputs the output from the microscope. A control method of a microscope system for observing a sample image formed based on light from a sample,
And a control process for outputting light having a wavelength component other than the wavelength band of the light to be observed emitted from the specimen to the light emitting unit whose wavelength band of the emitted light is variable based on information on the specimen. Control method of microscope system. In the microscope system that observes the sample image formed based on the light from the sample,
A control procedure for causing a light-emitting unit whose wavelength band of emitted light is variable to output light of a wavelength component other than the wavelength band of light to be observed emitted by the sample is executed based on information about the sample. A microscope system control program.

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