JP2016135774A - Composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide compositions that can maintain anti-oxidant action of Olea europaea L. extract.SOLUTION: We found out that compositions containing a phenylpropanoid glycoside, catechol compound, and Olea europaea L. extract in a specific ratio, or compositions in which Olea europaea L. fruit-juice extract and Olea europaea L. pulp extract are mixed as an Olea europaea L. extract can maintain anti-oxidant action of Olea europaea L. extract unexpectedly. By the present invention, it became possible to provide an Olea europaea L. extract composition with high commodity value. The forms of the composition of the invention are drinks, powders, powder drinks, tablets, jellies, hard or soft capsules, and cosmetics.SELECTED DRAWING: None

Description

  The present invention relates to a composition having an improved antioxidant effect of olive extract.

Polyphenols having an antioxidant action are said to be good for the body because they suppress oxidative stress in the body. Olives are rich in antioxidant polyphenols and have been used for food and medicine since ancient times. In addition, among the antioxidant polyphenols contained in olives, hydroxytyrosol has been reported to support the effect of inhibiting vascular LDL oxidation and the effect of preventing arteriosclerosis (Non-patent Document 1). Therefore, if an effective amount or more of hydroxytyrosol is continuously ingested, the LDL oxidation inhibitory effect of blood vessels and the prevention and improvement of arteriosclerosis can be expected.
In recent years, raw materials obtained by extracting each part of olive are commercially available so that an antioxidant polyphenol derived from olive can be efficiently ingested. However, because polyphenols have an antioxidant action, they are easily oxidized and unstable. Conventionally, a method for suppressing fading or decomposition of astaxanthin and resveratrol on melon SOD active extract coated with gliadin (Patent Document 1), salt-containing food processing residual liquid and salt derived from residue A method (Patent Document 2) that maintains the polyphenol reduced state and maintains the antioxidant action of polyphenol by allowing it to remain to some extent has been reported. However, these methods still have problems in terms of cost and flavor.

Zrelli, H. W. et al .: Eur. J. Pharm., 660, 275 (2011).

JP 2012-236794 A JP 2012-205554 A

  An object of the present invention is to provide a composition capable of maintaining the antioxidant action of an olive extract.

  As a result of intensive studies to solve the above problems, the inventors of the present invention contain a phenylpropanoid glycoside, a catechol compound, and an olive extract, and a composition containing these in a specific ratio, or an olive extract Surprisingly, it was found that a composition obtained by mixing an olive juice extract and an olive pulp extract as a product can maintain the antioxidant action of the olive extract, and the present invention has been completed.

That is, the present invention
(1) (a) a phenylpropanoid glycoside, (b) a catechol compound, and (c) an olive extract, wherein the content of the catechol compound is 0.5 mass relative to 1 part by mass of the phenylpropanoid glycoside Part or more of a composition,
(2) (a) Verbascoside and / or echinacoside, (b) hydroxytyrosol and / or caffeic acid, and (c) olive extract, the content of (b) is (a) 1 part by mass A composition that is 0.5 parts by weight or more,
(3) The composition according to (1) or (2), wherein the olive extract of (c) is an olive fruit extract,
(4) The composition according to (3), wherein the olive fruit extract comprises an olive juice extract and an olive pulp extract,
(5) a composition comprising an olive pulp extract and an olive juice extract;
(6) The composition according to (2), wherein hydroxytyrosol is formulated so as to be ingested in an amount of 5 mg or more per adult per day,
It is.

  According to the present invention, it is possible to provide a composition that suppresses a decrease in the antioxidant effect of the olive extract over time.

  Examples of the phenylpropanoid glycoside of the present invention include vervascoside and echinacosid. Examples of the catechol compound of the present invention include hydroxytyrosol and caffeic acid.

The olive extract of the present invention is an extract that is separated from natural olive (Olea europaea), and examples of the separation location include fruits (fruit pulp, fruit juice, seeds), leaves and the like. A preferred site in the present invention is a fruit.
What is necessary is just to select a commercially available olive fruit extract suitably as an olive fruit extract of this invention. For example, Olivex HT6 (fruit juice extract, extraction method: ethanol extraction (olive fruit juice obtained by squeezing olive fruit, acid-treated, filtered and purified with ethanol), total polyphenol content: 30 mass % Or more, hydroxytyrosol: 6% by weight or more, tyrosol: 1% by weight or more), Oleaselect (pulp extract, extraction method: mixed solvent extraction of water and ethanol (from olive fruit to a mixed solvent of water and ethanol) Extracted and powdered), total polyphenol content: 30% by mass or more, verbascoside content: 5% by mass or more, hydroxytyrosol: 1.5% by mass or more), OPEXTAN (fruit extract, extraction method: Extraction of water and ethanol in a mixed solvent (extracted from olive fruit with a mixed solvent of water and ethanol and powdered) 1), total phenol content: 10% by mass or more, Verbascoside content: 2.0-3.5% by mass content, hydroxytyrosol and its derivatives: 4.5% by mass or more), Hytolive (fruit extract, hydroxy Tyrosol content: 8% by mass or more), Polyphen-Oil (fruit extract, total polyphenol content: 6.5% by mass content, hydroxytyrosol or tyrosol content: 1.73% by mass content, verbascoside or oleuropein content: 0 .5 mass% content) can be used.

  The olive fruit extract of the present invention is more preferably formulated by combining an olive juice extract rich in hydroxytyrosol and an olive pulp extract rich in verbascoside. In addition, when the olive fruit extract is blended with the olive fruit extract and the olive fruit extract, 10 parts by weight of the olive fruit extract is used with respect to 1 part by weight of the olive juice extract in view of the effect of the present invention. It is preferable to mix | blend to the upper limit, More preferably, it is 0.54 mass part-10 mass parts, More preferably, it is 0.75 mass part-10 mass parts.

  The content of hydroxytyrosol or caffeic acid in the composition of the present invention is 0.5 parts by mass or more with respect to 1 part by mass of verbascoside or echinacoside from the viewpoint of the effect of the present invention. More preferably, it is 0.51 mass part-1.46 mass part, More preferably, it is 0.51 mass part-1.19 mass part.

  Moreover, it is preferable that the composition of this invention contains the monophenol compound further. Examples of the monophenol compound include tyrosol, tyrosine, oleocanthal and the like, and tyrosol is preferable. As a compounding quantity of a monophenol compound, 0.9 mass% or more is preferable in the composition of this invention.

  In addition, since the above-mentioned verbascoside, hydroxytyrosol and tyrosol are components contained in the olive extract, the olive extract extracted from each part such as pulp and fruit juice should be blended at an appropriate ratio so as to have the above ratio. That's fine. Apart from the olive extract, verbascoside, echinacoside, hydroxytyrosol, caffeic acid or tyrosol, and naturally occurring extracts containing these compounds may be added in the above proportions.

  In addition, when hydroxytyrosol in the composition of the present invention is formulated so that 5 mg or more per day is taken per adult, an antioxidative effect such as an inhibitory effect on LDL oxidation of blood vessels can be expected. . More preferably, it is 6 mg or more per day per adult.

  Examples of the form of the composition of the present invention include beverages, powders, powdered beverages, tablets, supplements, jelly, hard capsules or soft capsules, cosmetics, etc., preferably beverages, powders, powdered beverages, tablets to be taken orally. , Jelly, hard capsule or soft capsule.

  Hereinafter, the present invention will be described in more detail with reference to Examples, Comparative Examples and Test Examples. In addition, commercially available Olivex HT6 was used as the olive juice extract, and commercially available Oleaselect was used as the olive pulp extract. Moreover, the content of each component was measured by HPLC method.

Comparative Example 1
Olive juice extract was used.

Example 1
7 parts by mass of olive pulp extract was added to and mixed with 13 parts by mass of olive juice extract of Comparative Example 1.

Example 2
3 parts by mass of olive pulp extract was added to and mixed with 4 parts by mass of olive juice extract of Comparative Example 1.

Example 3
To 1 part by mass of the olive juice extract of Comparative Example 1, 10 parts by mass of olive pulp extract was added and mixed.

Example 4
1 part by mass of echinacoside was added to and mixed with 20 parts by mass of the olive juice extract of Comparative Example 1. That is, the composition contains 5.7% hydroxytyrosol and 4.8% echinacoside.

Example 5
3 parts by weight of caffeic acid was added to and mixed with 50 parts by weight of the olive pulp extract. That is, the composition contains 10.4% of hydroxytyrosol and caffeic acid in total and 10.4% of verbascoside.

  Table 1 below shows the contents (%) of verbascoside, echinacoside, hydroxytyrosol and caffeic acid contained in the compositions of Comparative Example 1 and Examples 1-3, and Table 2 shows the formulations of Examples 1-3. Show.

Test Example: Evaluation of Antioxidant Capacity of Composition (1) Method A certain amount of the composition shown in Comparative Example 1 and Examples 1 to 5 was weighed out and dissolved in dimethyl sulfoxide (manufactured by Kokusan Kagaku) and subjected to the test . Antioxidant ability evaluation was performed in a biological anti-oxidant potential test (BAP test kit: Diacron) using a free radical evaluation apparatus (FREE: DIACRON). Table 3 shows the rate of change in antioxidant capacity after storage at 85 ° C. for 6 days.

(2) Results As shown in Table 3, the rate of change in Examples 1 to 5 was more stable than that in Comparative Example 1. As the content of olive pulp or verbascoside increased, the decrease in the antioxidant capacity change rate was suppressed, and the result was the most effective in the ratio of the formulation of Example 3.
It has been found that the composition of the present invention can suppress a decrease in the antioxidant power of the olive extract over time. The contents of tyrosol in Comparative Example 1 and Examples 1 to 4 were all 1%, and Example 5 was 0.9%. The content of each component in the olive pulp extract used in the examples was measured by HPLC, and as a result, it was 11% verbascoside, 5% hydroxytyrosol, and 1% tyrosol.

Example 6
An olive fruit juice extract of Comparative Example 1 was prepared by adding an olive pulp extract so that each component had a content shown in Table 4. In addition, Table 4 shows the amount (mg) of each component when the preparation is made to have a daily dose of 110 mg.

  According to the present invention, it is possible to provide an olive extract blended composition having a high commercial value that suppresses a decrease in antioxidant effect over time. It can be used in the fields of medicine, food, functional labeling food, food for specified health use and cosmetics.

Claims (6)

(A) a phenylpropanoid glycoside, (b) a catechol compound, and (c) an olive extract, wherein the content of the catechol compound is 0.5 parts by mass or more relative to 1 part by mass of the phenylpropanoid glycoside A composition characterized by being. (A) Verbascoside and / or echinacoside, (b) hydroxytyrosol and / or caffeic acid, and (c) olive extract, the content of (b) being 0 (a) to 1 part by mass The composition which is 5 parts by mass or more. The olive extract of (c) is an olive fruit extract, The composition as described in (1) or (2), The composition according to claim 3, wherein the olive fruit extract comprises an olive juice extract and an olive pulp extract. A composition comprising an olive pulp extract and an olive juice extract. The composition according to claim 2, wherein hydroxytyrosol is formulated so as to be ingested in an amount of 5 mg or more per day per adult.