JP2016050911A - Bio-related substance collection device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a bio-related substance collection device capable of effectively wiping and collecting even fine bio-related substances.SOLUTION: The bio-related collection device comprises: a container body 3 which is deformable by compression, of which one end is closed, and in which a predetermined amount of diluted solution L is filled; a cap 15 which is detachably attached to an opening 5 of the container body 3, and has an attaching part 11 and an outlet 13; a shank 9 by which a base end part 17 is mounted to the attaching part 11 of the cap 15; and a wiping body 21 which is attached to the shank 9. Furthermore, the wiping body 21 comprises: a wiping operation part 25 which extends along the longitudinal direction of the shank; a collection sheet 29; and a pressing component 31 which is placed between the collection sheet 29 and the shank 9.SELECTED DRAWING: Figure 2

Description

  The present invention relates to a biological substance collection device used when examining biological substances in medical institutions, food, pharmaceutical manufacturing plants, etc., and particularly suitable for collecting minute biological substances such as norovirus. It relates to related substance collection equipment.

When collecting biologically relevant substances such as microorganisms and viruses, wipe the impregnated body composed of a cotton swab or a cotton ball as disclosed in Patent Document 1 below with a diluent such as a phosphate buffer. A wiping device for testing environmental microorganisms having a structure housed in a container body has been used.
In addition, the biological material collected with a cotton swab or such a special wiping device is impregnated again with the same solution as the diluent or with the diluent, and mechanically or by ultrasonic waves that do not have sterilizing power. It is vibrated and the leachate is taken out as a test solution. Furthermore, after culturing the taken-out test solution, the colonies of the bio-related substances that serve as specimens are measured, and the number of bio-related substances existing in the wiped area is calculated to inspect the bio-related substances.

  And in patent document 1, it has the cylindrical container main body in which the dilution liquid is accommodated, the one end of this container main body is obstruct | occluded with the detachable cover body, and the other end is also a detachable bottom cover body. The lid body is integrally formed with a stick portion extending toward the inside of the container body, and a wiping impregnation body constituted by a cotton ball is provided at the tip of the stick portion. Further, the bottom lid body is provided with a test solution outlet port in which a filter is arranged, and is further attached in a state where the cap body can be opened and closed via a hinge, and protrudes into the cap body. There is disclosed a wiping device for testing environmental microorganisms having a structure capable of closing the test solution outlet by a stopper provided in a state.

JP 2011-69778 A

However, in the swab and the wiping device for environmental microbiological examination disclosed in Patent Document 1 above, a cotton ball having a diameter of about 7 mm is obtained by rounding cotton into a ball as a wiping body to be collected by directly touching a biological substance at a test site. As a result, the wiping area was small, and the amount of wiping of biological substances that could be wiped by a single wiping operation was also reduced.
Moreover, since the fiber diameter of cotton is as large as about 30 μm, and the gap between the fibers is increased accordingly, it is difficult to collect minute biological substances such as viruses. In addition, in the conventional wiping device using a cotton ball as a wiping body, a slightly larger amount of phosphate buffered saline is required as a diluent, giving an impression that there is a large amount in appearance, The density | concentration of the extract | collected biological substance impregnated in this dilution liquid became low, and the test | inspection precision of the biological substance was reduced.

  Furthermore, as shown in FIGS. 4B and 10, in the conventional wiping device 101, the biological portion is impregnated in the attachment portion 107 of the shaft portion 105 that is a support member of the wiping body 103 constituted by cotton balls. Since the gaps 109 for taking out the liquid L to the outside are arranged radially, the structure is complicated and the manufacturing cost of the mold used for forming the mounting portion 107 is increased.

  Then, an object of this invention is to provide the biological material collection tool which can improve the test | inspection precision of a biological material. In addition, the present invention provides a bio-related substance collecting device that can wipe off bio-related substances efficiently by increasing the wiping area of bio-related substances and increasing the amount of bio-related substances that can be wiped by a single wiping operation. The purpose is to do. A further object of the present invention is to provide a biologically relevant substance collecting device that can wipe off minute biologically related substances by reducing the gap between fibers on the surface of the wiping action part.

The biological material collection device according to the first aspect of the present invention has a container body that is closed at one end and can be filled with a predetermined amount of diluent, and is detachably attached to the opening of the container body. A cap that includes a mounting portion that holds the shaft portion of the wiping member, a cap that has a discharge port through which the diluted solution containing the biological substance passes through the internal space of the mounting portion, and the cap. A biologically-related substance collecting device comprising: a shaft part to which a base end part is attached to an attachment part; and a wiping member provided with a wiping body for collecting a biologically-related substance at a distal end part of the shaft part, The wiping body has a wiping action portion extending in the longitudinal direction of the shaft portion that is in direct contact with the inspection site, and a latch that extends from the front and rear ends of the wiping action portion toward the shaft portion and is locked to the shaft portion at the tip end portion. And the And obtain collection sheet, which comprises a, a pressing member having a predetermined shape disposed between the blood collection preparative seat and the shaft portion.
According to this configuration, since the wiping area of the biological material is larger than that of the conventional wiping device that uses the cotton ball as a wiping body, the amount of wiping of the biological material that can be wiped by a single wiping operation is reduced. As the number increases, biological materials can be efficiently wiped off. Further, by providing the pressing member, it is possible to press the sampling sheet against the examination site with a uniform and strong pressing force.

Moreover, like the 2nd aspect of this invention, with respect to the base end part of the said axial part, the dilution liquid containing the biological material in a container main body is guide | induced to the internal space of the attaching part provided in the said cap. A discharge channel, and one or a plurality of slits extending in the longitudinal direction of the shaft that communicates the discharge channel and the internal space of the container body, and the discharge channel and the slit are formed. It is possible to form an insertion portion that fits with an attachment portion provided on the cap by using a part of the proximal end portion of the shaft portion near the end portion.
According to this configuration, it is no longer necessary to provide a complicated structure for forming the discharge flow path on the side of the cap mounting portion, making it easier to manufacture the mold used for molding the cap, and the mold manufacturing cost. Can be reduced. Then, the diluent containing the biological substance in the container body passes through the slit exposed on the distal end side of the insertion portion, reaches the discharge channel inside the shaft portion, and further engages with the shaft portion. It is taken out from the outlet through the internal space of the cap mounting portion.

In addition, as in the third aspect of the present invention, when the both ends are fitted between the base end portion of the shaft portion and the mounting portion provided on the cap, there is a gap between them. Alternatively, it is possible to form a discharge flow path that guides a dilute solution containing a biological substance in the container body to the attachment portion using the gap.
Specifically, as in the fourth aspect of the present invention, the outer peripheral shape of the base end portion of the shaft portion and the inner peripheral shape of the attachment portion into which the base end portion is fitted are different. Is possible. Furthermore, as in the fifth aspect of the present invention, one of the cross sections of the outer peripheral shape or the inner peripheral shape is a square or a polygon, and the other cross section of the outer peripheral shape or the inner peripheral shape is a circle or an ellipse. Is possible.
And according to these configurations, the bio-related substance can be obtained simply by changing the shape of the shaft base end, which is relatively easy to form, without changing the shape or structure of the cap-side mounting part that is difficult to form. As a result, it becomes possible to smoothly remove the diluting liquid containing the metal, and it is possible to reduce the production cost of the mold used for molding the cap.

  Further, as in the sixth aspect of the present invention, it is possible to dispose a filter that prevents foreign matter from entering the internal space of the mounting portion with respect to the base end portion of the shaft portion. According to this configuration, solid matter such as dust and dust that interferes with the inspection is captured by the filter. Therefore, the diluent containing only the biological substance to be inspected flows into the attachment portion and is taken out.

  Further, as in the seventh aspect of the present invention, the side surface shape of the pressing member may be formed in a trapezoidal shape having a front-falling slope in which the height of the side on the distal end side is lower than the height of the side on the proximal end side. Is possible. According to this configuration, in the wiping posture in which the collection surface of the collection sheet is in close contact with the surface of the examination site, a gap is formed between the cap and the surface of the examination site. .

  Further, as in the eighth aspect of the present invention, the shaft portion and the pressing member can be constituted by an integrally molded product formed from a single material. According to this configuration, the shaft portion and the pressing member can be formed in the same process, and the assembly cost can be reduced as much as the assembly of the shaft portion and the pressing member is unnecessary.

Further, as in the ninth aspect of the present invention, an engagement slit that engages with a part of the pressing member is formed in at least one of the front portion and the rear portion of the collection sheet, and the engagement A protruding portion that protrudes outward from the engaging slit can be formed in a part of the pressing member that engages with the slit.
According to this configuration, the pressing member is not only held and held between the abdomen and the sampling sheet, but also includes the engagement slit formed with respect to the locking portion of the sampling sheet and the protruding portion of the pressing member. It is also held by the engaging action. Therefore, even when the pressing force that presses the surface of the examination site is applied to the pressing member when wiping off the biological substance, the pressing member is maintained in the state held by the sampling sheet without being detached from the sampling sheet.

Further, as in the tenth aspect of the present invention, the wiping action portion can be provided with a wiping action surface having a width of 5 to 10 mm, preferably 7 mm, and a length of 30 to 70 mm, preferably 50 mm.
According to this structure, the wiping area of the conventional wiping body is 350 mm ² , and the conventional wiping body is wiped in comparison with the conventional structure in which a cotton ball composed of an elliptical sphere having a diameter of 7 mm and a length of 9 mm is used as the wiping body. Since the area is roughly estimated to be about 49 mm ² , the area ratio between the two is 7: 1. According to this configuration, a significant increase in the amount of wiping off the biological substance can be expected.

Further, as in the eleventh aspect of the present invention, the fiber diameter of the surface of the collection sheet can be 1 to 10 μm, preferably 5 μm. According to this configuration, since the gap between adjacent fibers is significantly smaller than that of cotton fibers having a fiber diameter of about 30 μm, it is easy to collect minute biological materials such as viruses.
Further, as in the twelfth aspect of the present invention, the surface material of the collection sheet can be formed of synthetic fibers of 80% polyester and 20% nylon. According to this structure, the outstanding wiping performance with respect to the biological material by an ultrafine fiber is exhibited.

Further, as in the twelfth aspect of the present invention, the diluent filled in the container body is a mixed solution of phosphate buffered saline and a surfactant, and the filling amount of the diluent is 10 ml or less. , Preferably 5 ml or less.
According to this configuration, the conventional wiping device using a cotton ball requires about 10 ml of diluent, whereas the diluent is 10 ml or less, preferably 5 ml or less, and the amount is small in appearance. Can give an impression. In addition, it is possible to increase the concentration of the collected biological substance impregnated in the diluent and improve the inspection accuracy of the biological substance.

According to the biological material collecting device of the present invention, the wiping area of the biological material is increased, and the amount of wiping of the biological material that can be wiped by a single wiping operation is increased, so that the biological material can be efficiently wiped off. Will be able to.
Further, since the fiber diameter on the surface of the wiping action portion becomes fine, the gap between the fibers becomes small, and even a minute biological substance can be wiped off. In addition, since the amount of the diluent used can be reduced, it is possible to increase the concentration of the biological substance and improve the inspection accuracy of the biological substance.
Furthermore, by simplifying the structure of the cap that is difficult to mold, it is possible to keep the product cost of the entire biological material collection tool low.

It is a disassembled perspective view which shows the biological material collection tool which concerns on the 1st Embodiment of this invention. It is a sectional side view which shows the biological material collection tool which concerns on the 1st Embodiment of this invention. They are the side view (a) which shows the wiping off member of the biological material collection instrument which concerns on the 1st Embodiment of this invention, and bb sectional drawing (b). It is the bottom view (a) which shows the wiping member of the biological material collection instrument which concerns on the 1st Embodiment of this invention, and the bottom view (b) which shows the conventional cotton ball. Sectional drawing (a) which shows typically the space | gap between the fiber of the wiping action part surface of the biological material collection instrument which concerns on the 1st Embodiment of this invention, and the fiber, and the fiber of a conventional cotton ball, and fiber It is sectional drawing (b) which shows a space | gap typically. It is a sectional side view which shows the biological material collection instrument which concerns on the 2nd Embodiment of this invention. FIG. 7 is a cross-sectional view taken along line AA in FIG. 6 and a cross-sectional view taken along line BB in FIG. 6 showing a biologically relevant substance collecting device according to a second embodiment of the present invention. It is sectional drawing which shows the two types of fitting states of the axial part and attachment part of the biological material collection instrument which concerns on other embodiment of this invention. It is a sectional side view which shows the axial part of the biological material collection instrument which concerns on other embodiment of this invention, and the attachment part periphery of a cap. They are the side sectional view (a) which shows the connection state of the axial part and attachment part of the conventional wiping instrument, and bb sectional drawing (b).

The biological material collection device of the present invention will be described with reference to the drawings, taking two embodiments of the first embodiment and the second embodiment as examples.
In the following description, first, based on FIG. 1 and FIG. 2, an outline of the overall configuration of the biological material collection device of the present invention will be described by taking the biological material collection device according to the first embodiment of the present invention as an example. Next, based on FIGS. 3 to 5, the structure of the wiping member, which is a characteristic configuration of the biological material-collecting device, will be described together with the connection structure of the wiping member and the cap, and then the present invention. The operation mode of the wiping member will be specifically described together with the operation procedure of the biological material collection tool.

Furthermore, based on FIG.6 and FIG.7, the structure and operation | movement aspect of the biological material collection instrument which concern on the 2nd Embodiment of this invention are demonstrated centering on the difference with the said 1st Embodiment.
Finally, based on FIGS. 8 and 9, the configuration of the biological material-collecting device according to another embodiment of the present invention will be described focusing on differences from the first and second embodiments. To do.

[First Embodiment]
(1) Outline of the overall configuration of the biological material collection device (see FIGS. 1 and 2)
The biological material-collecting device 1 of the present invention is detachably attached to a container body 3 whose one end is closed and filled with a predetermined amount of diluent L, and an opening 5 of the container body 3; An attachment part 11 for holding the shaft part 9 of the wiping member 7; a cap 15 having an outlet 13 for taking out the diluent L containing the biological substance G through the internal space of the attachment part 11; A wiper provided with a shaft part 9 to which the base end part 17 of the shaft part 9 is attached to the attachment part 11 of the cap 15 and a wiping body 21 for directly collecting the biological substance G at the tip part 19 of the shaft part 9 This is basically configured by including the member 7.

The wiping body 21 includes a collection sheet 29 and a pressing member 31 having a predetermined shape that is disposed between the collection sheet 29 and the shaft portion 9. The collection sheet 29 is in direct contact with the examination site W and extends in the longitudinal direction of the shaft portion 9, and the wiping operation portion 25 extends from the front and rear ends of the wiping operation portion 25 toward the shaft portion 9. Locking portions 27F and 27R to be locked.
Further, in the living body related substance collecting instrument 1A according to the present embodiment, the container body 3 is constituted by a cylindrical member with the bottom 4 closed, and the end of the container body 3 where the opening 5 is formed. A male thread portion 6 for connecting to the cap 15 is formed on the outer periphery of the portion. Moreover, as a material of the container body 3, a soft synthetic resin such as high-density polyethylene is applicable as an example.

Further, the body 2 of the container body 3 between the male screw portion 6 and the bottom portion 4 is easier to compress and deform in the direction Y intersecting the axial direction X than the male screw portion 6 and the bottom portion 4. It is desirable to be thin. Further, the container body 3 is made transparent or translucent so that the amount of the diluent L in the container body 3 can be confirmed.
Moreover, as the diluent L filled in the container body 3, a mixed solution of phosphate buffered saline and a surfactant can be applied as an example. In the present embodiment, 5 ml of the diluent L is used as an example of the container body. 3 was used.

The cap 15 is a cylindrical member that is closed at one end, and the discharge port 13 described above is formed at the closed end. In addition, a lid 35 is attached to one end of the cap 15 in which the discharge port 13 is formed via a hinge 33 so as to be opened and closed.
The lid body 35 is constituted by a short cylindrical member having one end substantially closed with the same diameter as the cap 15, and the discharge port 13 is formed on the inner surface of the closed end of the lid body 35. A plug 37 that fits in is provided so as to protrude inward.

A female screw 39 that meshes with the male screw portion 6 of the container body 3 described above is formed on the inner periphery of the side body portion of the cap 15. An attachment portion 11 that holds the shaft portion 9 is provided on the inner surface of the closed end of the cap 15 so as to protrude inward.
Moreover, in this Embodiment, the simple cylindrical attachment part 11 with an internal diameter of 4 mm and height 10mm was installed as an example. Note that the cap 15, the hinge 33, and the lid 35 are formed by an integrally molded product of the same material as the container body 3 described above as an example, and the hinge 33 can be flexibly deformed. It is desirable that it be formed thinner than other parts.

(2) Structure of the wiping member (see FIGS. 3 to 5)
As described above, the wiping member 7 includes the shaft portion 9 and the wiping body 21 provided on the shaft portion 9. The wiping body 21 includes a wiping action portion 25, a collection sheet 29, and a pressing member 31. The collection sheet 29 includes two locking portions 27F and 27R extending from the front and rear ends thereof.
In this embodiment, a pipe material having a circular cross section is used as the shaft portion 9 as an example, and the base end portion 17 of the shaft portion 9 is diluted with the biological material G in the container body 3. A discharge channel 41 that guides the liquid L to the attachment portion 11 provided in the cap 15, and four slits 43 as an example extending in the longitudinal direction of the shaft portion 9 that communicates the discharge channel 41 and the container body 3. , Is provided.

Further, the portion close to the end portion of the base end portion 17 of the shaft portion 9 in which the discharge flow path 41 and the slit 43 are formed is fitted to the attachment portion 11 provided in the cap 15. An insertion part (fitting part) 45 is formed.
In this embodiment, since the shaft portion 9 is made of a pipe material, a through-hole penetrating in the longitudinal direction is formed at the center of the shaft portion 9. Therefore, in the present embodiment, the discharge passage 41 is formed by using this through hole. However, the shaft portion 9 can be formed by a solid body having an arbitrary shape. A discharge channel 41 can be separately formed at the base end portion 17 of the portion 9.

The collection sheet 29 of the wiping body 21 is obtained by adhering a fiber as a surface material to a reinforcing back surface material (back surface sheet) by hot melt or the like. The fibers constituting the surface material are formed so that at least the surface fiber diameter D1 is 1 to 10 μm, preferably 5 μm. In this embodiment, in order to realize the range of the fiber diameter D1, a sampling sheet For example, 29 surface materials are formed by synthetic fibers made of polyester and nylon. The surface material of the collection sheet 29 can be preferably composed of 80% polyester and 20% nylon. The back material of the collection sheet 29 can be preferably composed of a polyester film. By configuring the collection sheet 29 from the back surface material and the front surface material, the surface material and the back surface material of the collection sheet 29 are integrated. This integration makes it easy to form the collection sheet 29 and makes it difficult to separate the fibers of the surface material during wiping.
Accordingly, in this embodiment, the gap C1 between the adjacent fibers F1 and F1 is reduced as shown in FIG. 5A, and the conventional fiber diameter D2 is about 30 μm as shown in FIG. 5B. It is much smaller than the gap C2 between the cotton fibers F2 and F2.

In the present embodiment, the wiping action portion 25 of the collection sheet 29 has a width B1 of 5 to 10 mm, preferably 7 mm, and a length L1 of 30 to 70 mm, preferably 50 mm, as shown in FIG. It has a rectangular and substantially flat wiping surface. Therefore, if the wiping action part 25 has a wiping action surface having a width B1 of 7 mm and a length L1 of 50 mm, the wiping area A1 is 350 mm ² .
On the other hand, in the conventional wiping device 101 which used the cotton ball as the wiping body 103, the wiping body as shown in FIG. 4B constituted by an elliptical sphere having a diameter B2 of 7 mm and a length L2 of 9 mm. In the case of 103, the wiped area A2 is roughly estimated to be about 49 mm ^2, so the area ratio between the two is 7: 1. A significant increase in the amount of wiping can be expected.

Here, the “biologically related substance” used in the present specification is a substance to be separated, collected or detected contained in the sample at the test site W, and is a microorganism, virus, bacterium, nucleic acid, polysaccharide, protein. Any biological material that can be wiped off, such as small molecules.
Further, in the present embodiment, the pressing member 31 interposed between the shaft portion 9 and the collection sheet 29 has a side surface shape whose height H1 of the side on the distal end side is higher than the height H2 of the side on the proximal end side. It is also formed in a trapezoidal shape with a lower front slope.
Accordingly, there is a gap S between the cap 15 and the surface of the inspection site W in the wiping posture in which the wiping operation surface of the wiping operation portion 25 serving as the collection surface of the collection sheet 29 is in close contact with the surface of the inspection region W. This makes it easy to wipe the biological substance G. The pressing member 31 is preferably formed from a resin or the like that does not absorb the diluent L.

(3) Mode of operation of the wiping member (see FIGS. 3 to 5)
Next, in conjunction with the operation procedure of the biologically relevant substance collecting instrument 1A according to the present embodiment configured as described above, the operation mode of the wiping member 7 will be described separately for (A) wiping and (B) taking out. To do.
(A) At the time of wiping When the biological substance G existing in the examination site W is wiped off and collected, the female screw part 39 meshing with the male screw part 6 of the container main body 3 is loosened to remove the wiping member 7 from the container main body 3. Take out.

Next, the cap 15 and / or the shaft portion 9 is pinched with fingers and pressed so that the wiping action surface of the bottom surface of the wiping action portion 25 of the collection sheet 29 is brought into close contact with the surface of the examination site W. At this time, preferably, a strong pressing force P can be obtained by pressing the index finger against the upper surface of the shaft portion 9.
Moreover, since the clearance S is formed between the cap 15 and the surface of the inspection site W due to the presence of the pressing member 31 described above, the wiping member 7 can be moved smoothly. Then, by moving the wiping body 21 having the large wiping area A1 and the small fiber diameter D1 in the width direction, the biological substance G on the surface of the examination site W can be efficiently wiped and collected.

(B) At the time of taking out The wiping member 7 collected by wiping off the biological substance G is accommodated in the container body 3 again, and the male screw part 39 of the cap 15 is meshed with the male screw part 6 of the container main body 3. The opening 5 of the main body 3 is closed with a cap 15.
Next, in this state, the biologically relevant substance collection device 1A is mechanically vibrated or vibrated with a weak ultrasonic wave having no sterilizing power, and the collected biologically relevant substance G is leached into the diluent L. And after culture | cultivating this dilution liquid L as a test | inspection liquid, it takes out out of the container main body 3 with the following procedures.

In this case, a finger is put on the lid 35 and the lid 35 is rotated in the expanding direction about the hinge 33 to be opened. In this state, since the plug 37 fitted to the discharge port 13 is detached from the discharge port 13, the discharge port 13 is in an open state.
Then, when the container body 3 is tilted so that the discharge port 13 is at the bottom, the diluent L containing the biological substance G passes through the slit 43 formed in the base end portion 17 of the shaft portion 9, It reaches the central discharge flow path 41, passes through the internal space of the attachment portion 11 of the cap 15, and is taken out from the discharge port 13 of the cap 15.

In addition, the diluted solution L containing the biological substance G taken out to the outside is used for the inspection by measuring the colony of the biological substance G that has appeared after culturing and calculating the number per wiped area. The
And according to the biologically relevant substance collection device 1A according to the present embodiment configured as described above, the biologically relevant substance G that can be wiped by a single wiping operation by increasing the wiping area A1 of the biologically relevant substance G. It is possible to efficiently wipe off the biological substance G by increasing the amount of wiping.

Further, since the gap C1 between the fibers F1 and F1 on the surface of the wiping action portion 25 can be reduced, it is possible to wipe even a minute biological substance G such as a virus. Moreover, it becomes possible to reduce the usage-amount of the dilution liquid L, and by using the dilution liquid L of a small usage-amount, it becomes possible to raise the density | concentration of the biological substance G and to improve a test | inspection precision.
Moreover, since the shaping | molding of the cap 15 becomes easy by simplifying the structure of the attaching part 11 of the cap 15, it becomes possible to reduce the product cost of 1 A of biological material collection | recovery instruments. Furthermore, the use of the pressing member 31 having the predetermined shape and the wiping body 21 having a large wiping area improves the operability of the wiping member 7 and enables more efficient wiping of the biological substance G.

[Second Embodiment]
(1) Configuration and operation mode of the biological material collection device (see FIGS. 6 and 7)
The biological material collection tool 1B according to the second embodiment has basically the same configuration as the biological material collection tool 1A according to the first embodiment described above, and (A) the shaft portion. The connection structure of the pressing member, (B) the locking structure of the pressing member and the locking portion, and (C) the connection structure of the shaft portion and the mounting portion are different from those of the first embodiment.
Therefore, the description of the same configuration and operation mode as in the first embodiment is omitted here, and the above (A) to (A) to (A)-() unique to the second embodiment are different from the first embodiment. The description will focus on the configuration of the three points C) and its operation mode.

(A) Connection structure of shaft portion and pressing member That is, in the present embodiment, the shaft portion 9 and the pressing member 31 are formed of an integrally molded product formed from a single material. Therefore, the shaft portion 9 and the pressing member 31 can be cut out from, for example, a single plate-like member, and the molding cost of the shaft portion 9 and the pressing portion seat 31 can be reduced.

(B) Locking structure of pressing member and locking portion That is, in the present embodiment, the front end portion of the pressing member 31 is connected to both the locking portion 27F at the front portion of the collection sheet 29 and the locking portion 27R at the rear portion. As an example of engagement with the corner portion 49F and the corner portion 49R at the rear end, square hole-shaped engagement slits 47F and 47R are formed. The two corner portions engaged with the two engagement slits 47F and 47R are configured to protrude outward from the engagement slits 47F and 47R, and the protruding portion is a front protrusion. A portion 49F and a rear protruding portion 49R are formed.
Incidentally, by adopting such a locking structure of the pressing member 31 and the locking portions 27F and 27R, the holding force of the pressing member 31 becomes stronger than in the case of the first embodiment. Therefore, even when the pressing force P that presses the surface of the test site W is applied to the pressing member 31 when the biological substance G is wiped off, the pressing member 31 remains held by the collection sheet 29 without being detached from the collection sheet 29. Will come to be.

(C) Connection structure of shaft portion and attachment portion That is, in the present embodiment, when both are fitted between the base end portion 17 of the shaft portion 9 and the attachment portion 11 provided on the cap 15. A shape or structure in which a gap is formed between the two is formed, and a discharge channel 41 is formed that guides the diluent L containing the biological substance G in the container body 3 to the attachment portion 11 using the gap. ing.
Specifically, the outer peripheral shape of the base end portion 17 of the shaft portion 9 is a square shape, and the inner peripheral shape of the mounting portion 11 into which the base end portion 17 is fitted is a circular shape. Can be used as the discharge flow path 41.

Incidentally, when the connection structure between the shaft portion 9 and the attachment portion 11 having such a configuration is adopted, the molding can be performed without changing the shape and structure of the attachment portion 11 on the cap 15 side, which is difficult to form. By simply changing the shape or the like of the base end portion 17 of the shaft portion 9, the diluent L containing the biological substance G can be smoothly taken out.
And the biologically relevant substance collection device 1B according to the present embodiment configured in this way also exhibits the same operations and effects as the biologically related material collection device 1A according to the first embodiment described above, It is possible to provide the biologically relevant substance collection device 1B that has a simpler structure and is less likely to cause the pressing member 31 to drop off.

The above is the basic embodiment of the present invention, but the biological material-collecting device 1 of the present invention is not limited to the above-described embodiment, and is a part within the scope of the present invention. It is possible to change or omit the general configuration.
For example, as a shape or structure for forming a gap between the shaft portion 9 and the attachment portion 11 employed in the second embodiment, the base end of the shaft portion 9 as shown in FIG. Not only the aspect which makes the outer peripheral shape of the part 17 square but the inner peripheral shape of the attachment part 11 is circular, it is possible to employ | adopt other various aspects.

Specifically, as shown in FIG. 8A, the outer peripheral shape of the base end portion 17 of the shaft portion 9 is the same circle as the inner peripheral shape of the attachment portion 11, but extends in the axial direction X on the peripheral surface. By forming one or a plurality of grooves 51, it is possible to form a gap that can be used as the discharge channel 41.
Further, as shown in FIG. 8B, the outer diameter of the base end portion 17 of the shaft portion 9 is made smaller than the inner diameter of the mounting portion 11, and a cylindrical gap formed by this is used as an example by a net. It is also possible to fit the spacer 53 configured. In this case, the space formed between the meshes of the spacer 53 becomes the discharge flow path 41.

In addition, as shown in FIG. 9, it is possible to install a cylindrical filter 55 that can cover the base end portion 17 and the attachment portion 11 with respect to the base end portion 17 of the shaft portion 9. As the material of the filter 55, a non-woven fabric or the like that prevents entry of foreign matter such as dust and dust into the mounting portion 11 but allows entry of the diluent L containing the biological substance G can be applied. is there.
In addition, the configuration in which the outer peripheral shape of the container body 3 and the cap 15 described in the above embodiment is cylindrical is an example, and other configurations such as a rectangular tube having a rectangular cross section or other polygonal cross section, etc. It can also be formed into a shape. Further, the size and shape of the wiping action portion 25 are not limited to those described in the above embodiment, and can be appropriately changed according to the difference in size and shape of the container body 3 to be used.

  Further, the surface material and fiber diameter D1 of the collection sheet 29 are not limited to those described in the above embodiment, and various materials and fiber diameters D1 that can capture minute biological substances G such as viruses. Can be adopted. Further, the number of slits 43 described in the first embodiment is not limited to four, but may be one to three, or five or more. Further, the engagement slit 47 provided in the second embodiment is provided in both the front and rear locking portions 27F and 27R of the collection sheet 29, and can be provided only in any one of these. It is.

DESCRIPTION OF SYMBOLS 1 Living body related material collection instrument 2 Trunk part 3 Container main body 4 Bottom part 5 Opening part 6 Male screw part 7 Wiping member 9 Shaft part 11 Attachment part 13 Outlet 15 Cap 17 Base end part 19 Tip part 21 Wiping body 25 Wiping action part 27 Locking portion 29 Collection sheet 31 Holding member 33 Hinge 35 Cover body 37 Plug 39 Female screw portion 41 Discharge flow path 43 Slit 45 Insertion portion 47 Engagement slit 49 Protruding portion 51 Groove portion 53 Spacer 55 Filter L Diluent G Bio-related substance X Axial direction Y Crossing direction W Inspection site D Fiber diameter F Fiber C Cavity B Width (diameter)
L Length A Wiping area H Height S Clearance P Pressing force

Claims (15)

A container body that is closed at one end and can be filled with a diluent, and a cap that is detachably attached to the opening of the container body, and the diluent containing a biological substance is exposed to the outside. In a biological material collection device comprising a cap having a discharge port that can be taken out, a shaft portion attached to the cap, and a wiping body attached to the shaft portion to collect the biological material,
The wiping body includes a wiping action portion extending along a longitudinal direction of a shaft portion so as to come into contact with an examination site, and a locking portion for locking the wiping action portion to the shaft portion. Related substance collection equipment. In the biological material-collecting device according to claim 1,
A living body related substance collecting device comprising a pressing member disposed between the wiping body and the shaft portion. The biologically relevant substance collecting device according to claim 1 or 2,
One or more slits are formed in the base end portion of the shaft portion to guide the diluent in the container body to the outlet of the cap. In the biological material collection instrument according to any one of claims 1 to 3,
The living body related substance collecting instrument, wherein a proximal end portion of the shaft portion is fitted to an attachment portion provided on the cap. In the biological material collection instrument according to any one of claims 1 to 4,
A discharge flow path is formed between the base end portion of the shaft portion and the mounting portion provided on the cap when the both are fitted, and the discharge flow path allows the dilution liquid in the container body to be formed. Is guided to the discharge port. In the biological material-collecting device according to claim 5,
The living body related substance collecting instrument, wherein an outer peripheral shape of a base end portion of the shaft portion is different from an inner peripheral shape of the attachment portion into which the base end portion is fitted. The living body related substance collecting device according to claim 6,
One of the cross sections of the outer peripheral shape or the inner peripheral shape is a polygon, and the other of the cross sections of the outer peripheral shape or the inner peripheral shape is a circle or an ellipse. In the biological material collection instrument according to any one of claims 1 to 7,
A living body related substance collecting device, wherein a filter for preventing foreign matter from entering the discharge port is disposed between a base end portion of the shaft portion and the attachment portion. The biologically relevant substance collecting device according to claim 2,
A side surface shape of the pressing member is formed in a trapezoidal shape in which the height of the side on the distal end side is lower than the height of the side on the proximal end side. The biological material collecting device according to claim 2 or 3,
The living body related substance collecting device, wherein the shaft portion and the pressing member are integrally formed. The biological material-collecting device according to any one of claims 2, 9, and 10,
An engagement slit that engages with the pressing member is formed in the locking portion of the wiping body. The biologically relevant substance collecting device according to claim 11,
A living body related substance collecting device, wherein the pressing member engaged with the engagement slit is formed with a protruding portion that protrudes outward from the engagement slit. In the biological material collection instrument according to any one of claims 1 to 12,
The wiping action part has a wiping action surface having a width of 5 to 10 mm and a length of 30 to 70 mm. In the biological material collection instrument according to any one of claims 1 to 13,
The fiber diameter which comprises the said wiping member is 1-10 micrometers, The biological material collection tool characterized by the above-mentioned. In the biological material collection instrument according to any one of claims 1 to 14,
The wiping member is made of a fiber containing at least polyester and nylon, and is a biological material collecting device.