JP2012516013A5

JP2012516013A5 -

Info

Publication number: JP2012516013A5
Application number: JP2011546936A
Authority: JP
Filing date: 2010-01-20
Publication date: 2013-03-07
Anticipated expiration: 2030-01-20

Claims

A method of mass spectrometry,
Accumulating ions in an ion trap;
Isolating the target ions within the ion trap,
Fragmenting (fragmenting) at least some of the target ions in the ion trap to form a plurality of first fragment ions;
A step of moving said at least a portion of the first fragment ions, the fragmentation sucrose NSo location that is located either upstream or downstream of said ion trap,
Fragmenting at least a portion of the first fragment ions in the fragmentation device to form a plurality of second fragment ions;
Accumulating said second fragment ions .

The method according to claim 1, wherein the isolated target ion is a precursor ion or a parent ion, or a fragmented target ion.

A method according to claim 1 or claim 2 , wherein
The ion trap is operated in an operating mode and has an effective first low mass or low mass to charge ratio cutoff;
The fragmentation device is operated in an operating mode and has an effective second low mass or low mass to charge ratio cutoff;
The method wherein the second low mass or low mass to charge ratio cutoff is substantially lower than the first low mass or low mass to charge ratio cutoff.

A method according to any one of claims 1 to 3 , comprising
The ion trap comprises (i) a quadrupole rod set, (ii) a hexapole rod set, (iii) an octapole or more rod set, and (iv) one or more openings for transmitting ions when in use. Selected from the group consisting of an ion tunnel or ion funnel ion trap comprising a plurality of electrodes, (v) a two-dimensional or linear ion trap, and (vi) a three-dimensional or Paul ion trap,
And / or
The fragmentation device comprises (i) a quadrupole rod set, (ii) a hexapole rod set, (iii) an octapole or more rod set, and (iv) one or more openings that allow ions to pass through when used. A method selected from the group consisting of: an ion tunnel or ion funnel ion trap comprising a plurality of electrodes, (v) a two-dimensional or linear ion trap, and (vi) a three-dimensional or Paul ion trap .

A method according to any one of claims 1 to 4 , comprising
Since the ion trap has a different number of electrodes than the fragmentation device, or is structurally different from the fragmentation device, the ion trap has a first low mass cutoff for ions having a specific mass to charge ratio. the a, the fragmentation device have a second low mass cut-off that is different from the first low mass cut-off,
And / or
The ion trap comprises a first plurality of electrodes having a first spacing and / or opening size and / or diameter;
The fragmentation device comprises a second plurality of electrodes having a second spacing and / or opening size and / or diameter different from the first spacing and / or opening size and / or diameter. ,
Method.

The method according to any one of 請 Motomeko 1 to claim 5,
A step of storing at least a part of the second fragment ions in an ion storage device or an ion trap;
Discharging at least a portion of the second fragment ions from the ion storage device or ion trap and sending the released second fragment ions to a mass analyzer for mass analysis.

A method according to any one of claims 1 to 6 , comprising
The method further comprises the step of applying a pulsed gas to the ion trap and / or the fragmentation device.

A mass spectrometer comprising:
An ion trap, and a fragmentation device disposed on either the upstream side or the downstream side of the ion trap,
In an operation mode, ions are accumulated in the ion trap, target ions are isolated in the ion trap, and at least some of the target ions are fragmented in the ion trap to form a plurality of first fragment ions And moving at least some of the first fragment ions to the fragmentation device and fragmenting at least some of the first fragment ions in the fragmentation device to form and accumulate a plurality of second fragment ions A mass spectrometer.

The mass spectrometer according to claim 8 , wherein the isolated target ion is a precursor ion, a parent ion, or a fragmented target ion.

A mass spectrometer according to claim 8 or claim 9 , wherein
In the mode of operation, the ion trap has an effective first low mass or low mass to charge ratio cutoff;
The fragmentation device has an effective second low mass or low mass to charge ratio cutoff;
A mass spectrometer wherein the second low mass or low mass to charge ratio cutoff is substantially lower than the first low mass or low mass to charge ratio cutoff.

A mass spectrometer according to any one of claims 8 to 10 ,
The ion trap comprises (i) a quadrupole rod set, (ii) a hexapole rod set, (iii) an octapole or more rod set, and (iv) one or more openings that allow ions to pass through when used. Selected from the group consisting of an ion tunnel type or ion funnel type ion trap each having a plurality of electrodes ,
And / or
The fragmentation device comprises (i) a quadrupole rod set, (ii) a hexapole rod set, (iii) an octapole or more rod set, and (iv) one or more openings that allow ions to pass through when used. Selected from the group consisting of an ion tunnel type or ion funnel type ion trap each having a plurality of electrodes,
Mass spectrometer.

A mass spectrometer according to any one of claims 8 to 11 , comprising:
Since the ion trap has a different number of electrodes than the fragmentation device, or is structurally different from the fragmentation device, the ion trap has a first low mass cutoff for ions having a specific mass to charge ratio. the a, the fragmentation device have a second low mass cut-off that is different from the first low mass cut-off,
And / or
The ion trap comprises a first plurality of electrodes having a first spacing and / or opening size and / or diameter;
The fragmentation device comprises a second plurality of electrodes having a second spacing and / or opening size and / or diameter different from the first spacing and / or opening size and / or diameter. ,
Mass spectrometer.

A mass spectrometer according to any one of claims 8 to 12 ,
And an ion storage device or an ion trap for storing at least a part of the second fragment ions,
In a mode of operation, a mass spectrometer wherein at least some of the second fragment ions are ejected from the ion storage device or ion trap and sent to a mass analyzer for mass analysis.

A mass spectrometer according to any one of claims 8 to 13 , comprising
Further, a mass spectrometer comprising a device for applying a pulsed gas to the ion trap and / or the fragmentation device.

A computer readable medium having stored thereon computer executable instructions,
The instructions can be executed by a mass spectrometer control system comprising an ion trap and a fragmentation device disposed either upstream or downstream of the ion trap;
The computer executable instructions are sent to the control system,
(I) accumulate ions in the ion trap;
(Ii) isolating the target ions in the ion trap;
( Iii) fragmenting at least some of the target ions in the ion trap to form a plurality of first fragment ions;
( Iv ) moving at least a portion of the first fragment ion to the fragmentation device;
( V ) fragmenting at least a portion of the first fragment ions in the fragmentation device to form a plurality of second fragment ions ;
(Vi) accumulate the second fragment ions ;
A computer-readable medium that operates as described above.