JP2012241186A - Dried and sterilized powder of moringa belonging to moringaceae, liquid extraction and oxidation rot preventing method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an extracted liquid containing a useful component from stem, root, leaf and seed of Moringa belonging to Moringaceae and to provide a low molecular vegetable oil.SOLUTION: The dried sterilized powder of the stem, root, leaf and seed of the Moringa is obtained by permeating a natural mineral ion-containing pineapple enzyme fruit juice liquid to the stem, root, leaf, seed of the Moringa to impregnate and thereafter, treating in a super heated vapor drying and sterilizing device and pulverizing. The useful component-extracted liquid is obtained from the powder with a hot water extraction liquid using a normal method. The low molecular vegetable oil is obtained by a compression extraction of the seed.

Description

The present invention relates to sterilized moringa stems, roots, leaves, and seeds that belong to the Wasabiaceae family, to dry sterilized powder by a superheated steam dry sterilizer, and to moringa extract extracted from the dry sterilized powder and oxidation rot prevention. For more details, please refer to Wasabiaceae which makes it easy to extract useful ingredients by preventing oxidative decay and coagulation / hardening of useful ingredients such as lipids, proteins, amino acids, carbohydrates, dietary fibers and vitamins of Moringa belonging to Wasabiaceae. The present invention relates to a dry sterilized powder of moringa stalk, root, leaf, and seed, an extract extracted from the dry sterilized powder, and a low molecular weight vegetable oil obtained by press extraction of seeds.
More specifically, the present invention relates to a basic agent used for food additives, soft drinks, cosmetics, health foods, pharmaceuticals, and various feed additives.
Note that the superheated steam drying sterilizer is a device that produces a reducing action, not an oxidizing action, because only a small amount of oxygen (several PPm) is contained in the steam.

Moringa belonging to the Wasabiaceae family is reproductively cultivated from Southeast Asia such as Indonesia, Thailand, Vietnam, India and Malaysia to Madagascar, East Africa, the Arabian Peninsula, and the tropical and subtropical regions of India. In Japan, there are Okinawa and Kyushu regions. Since it is difficult to ensure freshness after the harvest in the country of origin, it is difficult to ensure the freshness, so the current situation is that it is stored in a refrigerator at the same time as harvesting or is dried at the same time as harvesting.
Furthermore, the drying process is a normal hot-air drying process, the drying temperature is 100 ° C. to 120 ° C., 40 to 60 minutes, and the drying time is long, and the components are halved due to the oxidative discoloration of Moringa stems, roots and leaves belonging to Wasabiaceae.・ There is a state that is easy to be destroyed.

Until now, after adjusting to the range of bulk specific gravity of 0.45-0.5 g / cm ³ of seeds obtained by roasting Moringa seeds belonging to Wasabiaceae at 120-140 ° C., oil is obtained by pressing. A method for producing moringa oil having excellent oxidation stability is disclosed in (Patent Document 1).

(Patent Literature 1) is a pretreatment step performed before squeezing moringa oil, and is to produce fats and oils with higher antioxidant properties by roasting in a specific temperature range. In addition, improper synthesis of pressing resulting from overdrying of seeds and lowering of specific gravity by roasting at a high temperature of 120 to 140 ° C. by increasing the bulk specific gravity by means such as adding steam or water or adding vegetable oil seed meal, The process of roasting at 120 to 140 ° C., as described above, is performed when the vegetable oil is squeezed and extracted from seeds and fruits. A heating step is essential in order to separate the oil from the plant tissue, especially in most cases obtained through an extraction step. In general, the roasting temperature in the heating step for collecting vegetable oil from seeds and fruits is currently in the range of 120 to 160 ° C, so that the roasting is particularly performed at 120 to 140 ° C. There is a problem to describe in.

The heating method when these vegetable oils are squeezed and collected is particularly softened by adding steam or water to the cooker and, if necessary, adding 2 to 3% by weight of plant seed meal or corn compressed meal. By increasing resistance, being oiled has a lack of basic problem awareness that reduces the extractability of useful ingredients.
Furthermore, the improper synthesis of pressing resulting from overdrying and lowering of specific gravity is increased by increasing the bulk specific gravity by means such as charging steam or water or adding vegetable oil seed pods, while ensuring the feed amount to the press and the amount of oil collected. It is in. Due to overdrying and reduction in specific gravity, there is a problem that the oxidation of the original seed is accelerated in order to dry with steam, water, or vegetable oil seed meal.

JP 2006-57034 A (P2006-57034A)

Problems to be solved by the invention

  The present invention is useful by collecting moringa stems, roots, leaves, and seeds belonging to the family Wasabiaceae, immersing and impregnating them in pineapple enzyme juice containing natural mineral ions, preventing oxidative decay, and drying them with a superheated steam drying sterilizer. Promotes softening of ingredients, suppresses coagulation / hardening of proteins, low molecular weight amino acids, peptide amino acids, proteins, carbohydrates, lipids, dietary fiber, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin C, vitamin E The objective is to establish dry sterilized powder containing calcium, magnesium, phosphorus, potassium, zinc, copper, iron, and the like, and an extract extracted from the dry sterilized powder and a technique of low molecular weight vegetable oil by press extraction of seeds.

Means for solving the problem

  The present invention relates to a pineapple enzyme and a moringa stalk, root and leaf belonging to Wasabiaceae when it is impregnated and impregnated with a natural mineral ion-containing pineapple enzyme juice after collecting the stem, root, leaf and seed of moringa belonging to Wasabiaceae・ Combined with the seed complex protein substrate to become an initial hydrolysis state, and by applying external energy to sterilize it by superheated steam drying, the enzyme catalyst is promoted and the stem and root of Moringa belonging to Wasabiaceae・ Hardening and coagulation of dried moringa-containing components of leaves and seeds is suppressed, and low molecular amino acids, peptide amino acids, proteins, carbohydrates, lipids, dietary fiber, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin C, vitamins E to obtain dry sterilized powder containing calcium, magnesium, phosphorus, potassium, zinc, copper, iron, etc. And low concentration amino acid, peptide amino acid, protein, carbohydrate, lipid, dietary fiber, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin C, vitamin E, calcium, magnesium, phosphorus, potassium, zinc Thus, extraction of low molecular weight vegetable oil by extraction from a dry powder of copper, iron, etc. and squeezing of seeds was enabled, and the present invention was completed.

In the present invention, moringa stems, roots, leaves, and seeds belonging to the family Wasabiaceae contain a lot of saturated fatty acids. Therefore, they belong to the Wasabiaceae family in pineapple enzyme juice used as a freshness-preserving (anti-drip) and oxidative anti-corrosion agent. When impregnated with moringa stems, roots, leaves and seeds and dried in the sun, moringa stalks, roots, leaves and seeds belonging to the family Wasabiaceae did not have a bad smell and did not become hardened. Enzymatic extraction of moringa stems, roots, leaves, and seeds belonging to the family Wasabiaceae, low-molecular amino acids, peptide amino acids, proteins, carbohydrates, lipids, dietary fiber, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamins It was found that C, vitamin E, calcium, magnesium, phosphorus, potassium, zinc, copper, and iron are contained in a large amount in the extracted component.
Furthermore, it was found that the components obtained by immersing and impregnating seeds in a natural mineral-containing pineapple enzyme juice and then drying and pressing and extracting the vegetable oil are unique low molecular weight vegetable oils.
However, Moringa belonging to the family Wasabiaceae is reproductive in tropical and subtropical regions, so there is a possibility that general bacteria and Escherichia coli are attached, and superheated steam drying sterilization treatment also serves as sterilization, lipid, protein, Added to increase the concentration of extracted components such as amino acids and carbohydrates.

  That is, the present invention, after collecting moringa stems, roots, leaves and seeds belonging to Wasabiaceae, permeated and impregnated pineapple enzyme juice containing natural mineral ions, and once a substrate of a protein catalyzed by pineapple enzyme After binding to enzyme molecules and facilitating hydrolysis reaction, by applying external energy to sterilize this by superheated steam drying, the protein is decomposed into various amino acid components and converted into peptide amino acids, which accelerates the hydrolysis reaction. A dry sterilized powder of moringa stems, roots, leaves and seeds belonging to the family Wasabiaceae, which has been subjected to a dry sterilization treatment in a soft and efficient state, is obtained. The dry sterilized powder can be easily extracted in a high temperature and high pressure extraction method at 110 to 115 ° C. for 20 to 25 minutes. In the extraction solution, low molecular amino acids and peptide amino acids histidine (His), lysine (Lys), tryptophan ( Trp), phenylalanine (Phe), methionine (Met), threonine (Thr), leucine (Leu), isoleucine (Ile), valine (Val), aspartic acid (Asp), serine (Sir), glutamic acid (Glu), proline (Pro), glycine (Gly), alanine (Ara), tyrosine (Tyr), arginine (Arg), β-aminobutyric acid (BABA), γ-aminobutyric acid (GABA) with high concentration composition and protein, lipid, carbohydrate, Dietary fiber, calcium (Ca), magnesium (Mg), phosphorus (P), potassium Obtaining low-molecular-weight vegetable oils and the like by pressing and extracting rhium (K), iron (Fe), zinc (Zn), copper (Cu), vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin C, vitamin E and seeds As a result, the present invention has been completed.

  Amino acid components and concentration analysis of the present invention were conducted by the Japan Food Analysis Center (No. 30604026) and (No. 306062065), (No. 30802664), (No. 306010437), Indonesia Bogor University (LT-405-541), ( Yes) conducted at SNR Research Institute (No. 200703320).

The present invention comprises the following configurations.
(1) Moringa stems, roots, leaves, and seeds belonging to Wasabiaceae are impregnated and impregnated with pineapple enzyme juice containing natural mineral ions, sterilized by superheated steam drying, and then crushed. Stem, root, leaf, seed dry sterilized powder, extract extracted from dry sterilized powder, and low molecular weight vegetable oil by pressure extraction of seeds.
(2) Calcium, sodium, magnesium, potassium obtained by immersing pineapple enzyme juice containing natural mineral ions into natural ore leeches, barleystone, heteroeolite, or zeolites in pineapple peel juice containing plant enzymes. Of extract liquids and seeds extracted from dry sterilized powder and dried sterilized powder of Moringa stems, roots, leaves, and seeds belonging to Wasabiaceae, which are composed of mineral ions such as iron, aluminum, zinc, and silicon Low molecular weight vegetable oil by compression extraction.
(3) Before drying and sterilizing moringa stems, roots, leaves and seeds belonging to the family Wasabiaceae, impregnation with pineapple enzyme juice containing natural mineral ions to prevent oxidative decay and to prevent malodors and to soften useful ingredients. A low-molecular weight vegetable oil obtained by extracting from a dry sterilized powder of a moringa stem, root, leaf, or seed belonging to the Wasabiaceae family described in (1) or (2) and a dry sterilized powder.
(4) Useful components of dry sterilized powder of Moringa stems, roots, leaves and seeds belonging to the family Wasabiaceae include low molecular amino acids, peptide amino acids histidine (His), lysine (Lys), and tryptophan. (Trp), phenylalanine (Phe), methionine (Met), threonine (Thr), leucine (Leu), isoleucine (Ile), valine (Val), aspartic acid (Asp), serine (Sir), glutamic acid (Glu), proline (Pro), glycine (Gly), alanine (Ara), tyrosine (Tyr), arginine (Arg), β-aminobutyric acid (BABA), and γ-aminobutyric acid (GABA) Protein, lipid, carbohydrate, food fiber, calcium (Ca), magnesium (Mg), phosphorus (P), potash (K), Iron (Fe), Zinc (Zn), Copper (Cu), Vitamin A, Vitamin B1, Vitamin B2, Vitamin B3, Vitamin C, Vitamin E, which is easily extracted at a high concentration (1), (2) A dry sterilized powder of Moringa belonging to the Wasabiaceae family described in (3), an extract extracted from the dry sterilized powder, and a low molecular weight vegetable oil obtained by press extraction of seeds.

  In the present invention, moringa stems, roots, leaves and seeds belonging to the family Wasabiaceae are collected from the reproductive cultivation area, and the freshness decreases in a short time. It is impregnated with pineapple enzyme juice containing natural mineral ions to prevent spoilage. This prevents the moringa from being oxidized by saturated fatty acids, and the protein is catalyzed by the pineapple enzyme and can easily be hydrolyzed.

Conventionally, in the field of moringa reproduction cultivation belonging to Wasabiaceae, it is transported to the factory at the same time as harvesting and refrigerated and stored, but when it is taken out from the refrigerator and dried, the temperature difference between the inside of the refrigerator and the outside air at the installation site of the drying equipment is large, which causes the drip phenomenon Discoloration oxidation due to. Those that have undergone discoloration oxidation are usually inspected and eliminated before drying because they reduce the components by half that cause the product color to deteriorate after drying. Therefore, the yield after drying is bad.
For handling moringa belonging to Wasabiaceae, the pineapple enzyme juice containing natural mineral ions of the present invention is dipped and impregnated with moringa belonging to Wasabiaceae, and oxidizes saturated fatty acids and proteins contained in Moringa belonging to Wasabiaceae Anti-corruption, amino acids, lipids, proteins, carbohydrates, dietary fiber, vitamins, etc. Of these, substances that are severely discolored due to oxidation are lipids and proteins, but pineapple enzyme juice containing natural mineral ions is immersed and impregnated with a moringa belonging to Wasabiaceae to prevent oxidative decay and maintain freshness. Is the first finding by the inventor.

Moringa stems, roots, leaves, and seeds belonging to Wasabiaceae, which are obtained by impregnating and impregnating a pineapple enzyme fruit juice containing natural mineral ions into a harvest of Moringa belonging to the Wasabiaceae family of the present invention, and then subjecting it to superheated steam drying sterilization treatment. The components of the dried product can be obtained from a fine powder by a food pulverizer without being hard or solidified.
Furthermore, a high-concentration extract can be obtained from each dry powder by a high-pressure extraction method.
The seeds can be extracted with a press after sterilization by superheated steam drying to obtain a low molecular weight vegetable oil.

  The present inventor stated that the dried product of Moringa belonging to the Wasabiaceae family was not hardened or solidified because the hydrolysis reaction of Moringa proteins and lipids belonging to the Wasabiaceae family was not only hydrolyzed by wet treatment but also resistant to high heat. As a result of promoting the hydrolysis reaction of proteins and lipids impregnated with the sexual pineapple enzyme, it is considered that low molecular weight amino acids and peptide amino acids can be hydrolyzed.

Effect of the invention

  The present invention relates to moringa stems, roots, leaves and seeds belonging to the family Wasabiaceae, which are soaked and impregnated with pineapple enzyme juice containing natural mineral ions in the stems, roots, leaves and seeds belonging to the family Wasabiaceae.・ From seed protein to low molecular weight amino acids, 18 peptide amino acid components, β-aminobutyric acid, γ-aminobutyric acid, protein, lipid, carbohydrate, dietary fiber, 6 vitamins, calcium, magnesium, phosphorus, potassium, zinc, It became possible to obtain low molecular weight vegetable oil by dry extraction pasteurized powder containing copper, iron, etc. and dry extract pasteurized powder and seed press extraction, food additives, cosmetics, health food, soft drinks, In addition to pharmaceuticals, the effects on industrial applications such as livestock feed, fish feed, and pet food are enormous.

As described above, in the present invention, the following effects can be obtained.
(1) (Massinaceae) by immersing and impregnating pineapple enzyme juice containing natural mineral ions into the stems, roots, leaves, and seeds of Moringa belonging to the Wasabiaceae family consisting of Prevents oxidative decay of saturated fatty acids and proteins of Moringa stems, roots, leaves and seeds belonging to the above, preserves freshness, promotes hydrolysis, and obtains low molecular weight amino acids, 18 peptide amino acids, β-aminobutyric acid from the obtained dry sterilized powder, Extraction obtained by liquid extraction of various useful ingredients such as γ-aminobutyric acid, protein, lipid, carbohydrate, dietary fiber, 6 vitamins, calcium, magnesium, phosphorus, potassium, zinc, copper, iron from powder and dry powder Low molecular weight vegetable oil is obtained by pressing and extracting liquid and seeds.

(2) (Natural mineral-containing pineapple enzyme juice consisting of (claim 2)) is the hydrolase of (1) above, and is a low molecular amino acid, peptide amino acid 18 from the dry sterilized powder. Species, β-aminobutyric acid, γ-aminobutyric acid, protein, lipid, carbohydrate, dietary fiber, 6 vitamins, calcium, magnesium, phosphorus, potassium, zinc, copper, iron It is done.
Furthermore, the effect of easily eluting low-molecular-weight vegetable oil by pressing and extracting seeds can be obtained.

(3) (Constitutional requirement of [Claim 3]) can also achieve the same effect as the above (2).

(4) From the dry sterilized powder of (1) and (2), which is composed of (constituent requirements of [Claim 4]), low molecular weight amino acids, peptide amino acids histidine (His), lysine (Lys) , Tryptophan (Trp), phenylalanine (Phe), methionine (Met), threonine (Thr), leucine (Leu), isoleucine (Ile), valine (Val), aspartic acid (Asp), serine (Sir), glutamic acid (Glu) ), Proline (Pro), glycine (Gly), alanine (Ara), tyrosine (Tyr), arginine (Arg), β-aminobutyric acid (BABA), γ-aminobutyric acid (GABA), protein, lipid, carbohydrate, food Fiber, 6 types of vitamin A, B1, B2, B3, C, E, calcium, magnesium, phosphorus, potassium The effect of facilitating liquid extraction of zinc, copper and iron at a high concentration can be obtained.
Furthermore, the effect of easily eluting low-molecular-weight vegetable oil by pressing and extracting seeds can be obtained.

FIG. 3 is an ESR (electron spin resonance) signal intensity (I) = h1 / h2 diagram of the first best mode for carrying out the present invention.

The present invention describes in detail the most preferred embodiment of the dry sterilized powder of Moringa stems, roots, leaves and seeds belonging to Wasabiaceae obtained from the stems, roots, leaves and seeds of Moringa belonging to Wasabiaceae having the above structure To do.
If the present invention is a moringa belonging to the family Wasabiaceae, it is reproductively cultivated from Southeast Asia such as Indonesia, Thailand, Vietnam, India and Malaysia to Madagascar, East Africa, the Arabian Peninsula, and tropical to subtropical regions of India. Yes. In Japan, there are Okinawa and Kyushu regions. Moringa belonging to all these horseradish families can be used.

  Moringa stems, roots, leaves, and seeds belonging to the above-mentioned Wasabiaceae are immersed and impregnated with natural mineral ion-containing pineapple enzyme juice before drying to prevent oxidation spoilage, to maintain freshness, and to perform hydrolysis pretreatment.

  The natural mineral ion-containing pineapple enzyme juice of the present invention will be described. Examples of the mineral containing a group of mineral ions include zeolite, barleystone, leechite, and Ioishi. In the present invention, mineral fine powders of metal oxides such as calcium, magnesium, potassium, sodium, iron, manganese, aluminum, zinc, silicon and titanium contained in diorite modified biotite called syllabus leechite are pineapple enzyme What was obtained by immersing in a juice and extracting in an ionized state is referred to as a natural mineral ion-containing pineapple enzyme juice in the present invention, and has been confirmed to have reducibility.

Table 1

び麦飯石の成分組成は、概して（表１）に示す混合粉末（１：１：１）を用いた。

Natural mineral ions will be described.

The mixed composition (1: 1: 1) shown in (Table 1) was generally used as the component composition of the barley rice stone.

Table 2

The mineral composition values of the natural mineral ion-containing pineapple enzyme juice used in the present invention were generally those shown in (Table 2).

Next, the pineapple enzyme juice will be described.
It is known that pineapple enzyme is contained in pineapple peel. The pineapple enzyme contains 30 or more kinds of enzyme components such as protein and peptide amino acid molecular degrading enzymes, various organic acid degrading enzymes, in addition to cellulose degrading enzymes. The pineapple enzyme contains enzymes such as dehydrogenation (oxidation) decarboxylation, deamine, etc., and by their combined catalytic action, peptide amino acids of moringa stem, root, leaf, and seed belonging to Wasabiaceae, β-aminobutyric acid , Γ-aminobutyric acid, proteins, lipids, carbohydrates, dietary fiber, etc., are broken down into single molecules and low molecules.

Table 3

＊パイナップル酵素は腐敗しやすいため、Ｈ_２ＳＯ_４、Ｈ_３ＰＯ_４を少量添加し酸性溶液となっている。
（表３）のパイナップル酵素成分の外、プロテアーゼ、アミラーゼ、リパーゼ、キナーゼ、マルターゼ、トランスアミラーゼ、デカルボキターゼ等を用いても差しつかえない。(Table 3) shows the main components of the pineapple enzyme.

* Since the pineapple enzyme is easily spoiled, a small amount of H ₂ SO ₄ and H ₃ PO ₄ are added to form an acidic solution.
In addition to the pineapple enzyme component of (Table 3), protease, amylase, lipase, kinase, maltase, transamylase, decarboxytase, etc. may be used.

  The mineral ion group in the natural mineral-containing pineapple enzyme juice of the present invention is impregnated and adsorbed to a useful composition of moringa belonging to the Wasabiaceae family together with the pineapple enzyme, and when dried, forms a dried product with hydroxyl groups, and cures the dried product. Indispensable for prevention.

天然ミネラルイオン含有パイナップル酵素果汁液中に長期間安定状態を維持し続け含有される。Next, the action of preventing oxidation and decay of the natural mineral ion-containing pineapple enzyme juice of the present invention will be described.
The present invention relates to the formation of an excited state when the metal oxide of the natural mineral ore described above is extracted in an ionic state by being immersed in an inorganic acid added for stable storage together with the organic acid contained in the pineapple enzyme juice. Water (H ₂ O) becomes excited hydrogen (H ·) and combines with surrounding water (H ₂ O)

It is contained in a pineapple enzyme juice containing natural mineral ions while maintaining a stable state for a long time.

It is considered that the oxidative decay prevention action of the natural mineral ion-containing pineapple enzyme juice of the present invention has the function of reducing the excited active hydrogen (H.) as well as the antioxidant.
In general, excited active hydrogen refers to the chemical dictionary editorial committee “Chemical Dictionary” (Kyoritsu Shuppan Co., Ltd.). Hydrogen in the nascent state is in an atomic state or a state close to it, causing a chemical reaction. It is described as referring to hydrogen that is becoming easier.
The excited active hydrogen of the present invention is also in an atomic state or a state close to an atomic state, but is considered to be a protonium ion (H ₃ O ⁺ ) without being converted to hydrogen gas (H ₂ ).

Existence of excited hydrogen or protonium ion (H ₃ O ⁺ ), which is a reducing property of pineapple enzyme juice containing natural mineral ions, was subjected to a copper dissolution test using metallic copper (Cu) to confirm the presence of reducing substances. .
It is known that the dissolution of copper ionizes with an oxidation reaction and becomes a blue copper ionic liquid. In the test, since the copper metal was not dissolved by the dilute sulfuric acid solution alone, when the copper piece was put into the nitric acid sulfuric acid solution in which a small amount of nitric acid was added to the dilute sulfuric acid solution, the copper piece was dissolved to become a blue liquid.
Next, when a small amount of the natural mineral ion-containing pineapple enzyme juice of the present invention was added to the nitric acid sulfuric acid solution to which nitric acid had been added, the copper pieces did not dissolve and the solution containing the copper pieces remained colorless. . Furthermore, when iron pieces (kugi) were put into this solution, the iron pieces continued to have a metallic luster without generating rust.
This is considered to be evidence showing the presence of excited hydrogen having a reducing ability to prevent oxidation in the pineapple enzyme fruit juice composition containing natural mineral ions.

The natural mineral ion-containing pineapple enzyme juice of the present invention is based on the knowledge that the above-mentioned excited active hydrogen (H.) works effectively in preventing oxidative decay. Applied to anti-corruption.
The mineral ionization of the metal oxides of natural ores in [Table 1] will be described in detail.
As an example, explanations of excited fresh water and excited active hydrogen (H.) generated while mineral ions are ionized with an acidic solution during leaching are shown in (1) and (2).
As an acidic solution, ionization of MgO and formation of hydronium ions (H ₃ O ⁺ ) with isovaleric acid [(CH 3) ₂ CHCHCO ₂ H], a pineapple organic acid.

Based on the basic test described above, the inventors of the present invention have demonstrated that the reducing substance of the natural mineral ion-containing pineapple enzyme juice is an excited hydrogen (H.) scavenger DPPH (1,1 Diphengl-2-piorhydrohydrazyl, max = 517 mn) radical spectrum. The ESR (electron spin resonance) method using the peak reduction of the ESR was measured by an ESR measuring device (JES-FE3 × GSpetrometer manufactured by JOEL) [Water Design Laboratory (School of Pharmacy)]. The reducing substance was confirmed to be atomic active hydrogen (H.).
The method for detecting excited hydrogen (radical hydrogen H.) of the invention uses the principle that DPPH radical (517 nm) disappears quantitatively when DPPH radical captures H. which has reducing performance based on the following reaction. Detection method.
The reaction formula is represented by the formula [Chemical Formula 1] and the signal intensity (I) h2 / h1 diagram is shown in FIG.

1

Reaction formula DPPH captures H · (active hydrogen) and disappears as DPPH-H

Reaction formula (3) shows that when a 500-fold diluted solution of pineapple enzyme juice containing natural mineral ions is used, excited active hydrogen (H.) reacts with DPPH to produce DPPH-H, and the DPPH radical peak decreases. (H1 / h2) FIG. 1 is a detection peak diagram showing [FIG. 1].
The amount of excited active water (H ·) reacted with DPPH was detected as 20.0 mgH / L.

The superheated steam drying sterilization method for moringa stems, roots, leaves, and seeds belonging to the Wasabiaceae family of the present invention will be described.
Superheated steam drying sterilizer (Econos Japan Co., Ltd.) Model: Using Super Steamer Mini Tester, Moringa stems, roots, leaves and seeds belonging to Wasabiaceae are immersed and impregnated in pineapple juice enzyme solution containing natural mineral ions Then, this was inserted into the apparatus, and superheated steam drying and sterilization treatment was carried out while maintaining the superheated steam temperature at 160 ° C to 180 ° C, and the sterling, roots, leaves and seeds of Moringa belonging to Wasabiaceae were sterilized. Small high-speed pulverizer (manufactured by Sansho Industry Co., Ltd.) Model: A dry sterilized powder of moringa stems, roots, leaves and seeds belonging to Wasabiaceae is obtained using NH-34 type.

  Hereinafter, the present invention will be described in more detail with reference to examples, but these examples do not limit the present invention.

  100 parts by weight of Moringa stalk belonging to Wasabiaceae and 50 parts by weight of natural mineral ion-containing pineapple enzyme juice 50-fold diluted solution are placed in an immersion container, stirred at room temperature and immersed for 60 minutes, and then the natural mineral ion-containing pineapple enzyme Move the stem of Moringa belonging to the juice-impregnated Wasabiaceae to the container, put it into the superheated steam drying sterilization treatment device set at 160 ° C-180 ° C, carry out the superheated steam drying sterilization treatment for 18-20 minutes, and dry 68 parts by weight of a stalk of Moringa belonging to the sterilized Wasabiaceae family was obtained. This was pulverized by a pulverizer to obtain a dry sterilized powder.

Comparative Example 1

  Moringa stems belonging to Wasabiaceae used in Example 1 were dried with a known drying apparatus without adding a pineapple enzyme juice containing natural mineral ions, and belonged to Comparative Example Wasabiaceae using a food grinder. After obtaining a dried moringa stalk, it is pulverized by a pulverizer to obtain a powder.

  About the dry sterilized powder of moringa stem belonging to Wasabiaceae obtained in (Example 1) and the usual dry treatment powder of Moringa stem belonging to Wasabiaceae obtained in (Comparative Example 1), the composition of amino acids and useful ingredients Quantitative analysis was performed by the Japan Food Analysis Center (supra). The results are shown in (Table 4) (Table 5).

Table 4

Table 5

(Table 4) From (Table 5), as a result of (Example) of the present invention, (Comparative Example) The powder is a moringa stem belonging to Wasabiaceae, and warm air without adding natural mineral ion-containing pineapple enzyme juice It is a dry powder of moringa stems belonging to the family Wasabiaceae. The powder of (Example) was obtained by immersing and impregnating a moringa stem belonging to Wasabiaceae in a pineapple enzyme juice containing natural mineral ions for 60 minutes, followed by sterilization treatment with superheated steam drying for 20 minutes. It is a dry sterilized powder. 218 kinds of low molecular weight amino acids and peptide amino acids are contained at a high content, showing a value close to 1.4 times that of the comparative example, and the protein value of useful components is lowered, and the content of lipid, carbohydrate and dietary fiber is high. Could get as. The decrease in protein and the increase in various amino acids is a proof that the protein is changed to a low molecular amino acid or a peptide amino acid in the Examples relative to the Comparative Example.
Furthermore, in the case of vitamins, calcium, magnesium, phosphorus, potassium, zinc, iron, etc., compared to the normally dried powder of (Comparative Example), as a result of (Example) of the present invention, Moringa belonging to Wasabiaceae The powder component obtained by immersing and impregnating the stem with natural mineral-containing pineapple enzyme juice and then superheated by steam drying and sterilization was found to have a high content value of 1.3 to 1.4 times.

Moringa stems belonging to Wasabiaceae obtained in (Examples) were immersed in natural mineral ion-containing pineapple enzyme juice for 60 minutes, sterilized by superheated steam drying, and then 100 parts by weight of the pulverized powder was extracted by 200 wt. The mixture was stirred in a sealed state for 30 minutes at 100 ° C. to 120 ° C. to extract components, the extract was filtered through a 200 mesh filter, and the extracted solution was further heated and concentrated at 100 ° C. for 30 minutes to 120 weights of the component extraction solution. Got a part. Further, the obtained extract was heated at 80 ° C. for 50 minutes to obtain 100 parts by weight of the concentrated solution.
Furthermore, (Comparative Example) The powder was prepared by adding 100 parts by weight of a powder obtained by subjecting Moringa stalks belonging to Wasabiaceae to a hot air dry treatment without adding a natural mineral ion-containing pineapple enzyme juice to 200 parts by weight of a conventional warm water extract. The mixture was stirred and sealed for 30 minutes at 100 ° C to 120 ° C, extracted components, the extract was filtered through a 200 mesh filter, and the extracted solution was heated and concentrated at 100 ° C for 30 minutes. Obtained. Further, the obtained extract was heated at 80 ° C. for 50 minutes to obtain 100 parts by weight of the concentrated solution.

For the liquid extract obtained by drying the moringa stem belonging to Wasabiaceae obtained by the method of Example 3, quantitative analysis of amino acid and useful component concentrations was analyzed by Japan Food Analysis Center (supra). Carried out.
The results are shown in (Table 6) (Table 7).

Table 6

Table 7

(Table 6) From the results of amino acid component analysis of Moringa stem powder extract obtained in (Example 3) from (Table 6), the Moringa stem dry sterilized powder belonging to Wasabiaceae obtained in (Example) of the present invention. 99% or more of the low molecular amino acids and peptide amino acids contained were extracted, and the total molecular weight of isolated molecular amino acids was 10,004 mg / 100 g. Thus, a high concentration extract composition value of low molecular amino acids could be obtained.
That is, the amino acids arginine 540.5 mg, lysine 730.7 mg, histidine 387.6 mg, phenylalanine 414.9 mg, tyrosine 36 mg, leucine 1,259.6 mg, isoleucine 541.9 mg, methionine 355.6 mg, valine in 100 g of the extract 730.1 mg, alanine 411.3 mg, glycine 42.3 mg, proline 338.7 mg, glutamine 3.8 mg, glutamic acid 326.9 mg, serine 428.8 mg, threonine 1,844.6 mg, γ-aminobutyric acid GABA 1,022. A total of 10,004 mg of total amino acids of 3 mg, aspartic acid 83.8 mg, tryptophan 441.5 mg, and β-aminobutyric acid 62.1 mg were detected.
Furthermore, from Table 7, protein, carbohydrate, lipid, dietary fiber, calcium, magnesium, phosphorus, potassium, zinc, copper, iron, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin C, vitamin E 99% was also extracted.
The number of general bacteria, coliform bacteria, Escherichia coli, Staphylococcus aureus and thermostable spore bacteria in the extract was all negative.

  The present invention is a sterilized dry sterilized powder obtained by immersing and impregnating a pineapple enzyme juice containing natural mineral ions separately into moringa stems, roots, leaves and seeds belonging to Wasabiaceae, followed by superheated steam drying sterilization treatment More extracted moringa components belonging to Wasabiaceae, which show a high content of low molecular amino acids and peptide amino acids in particular, making it possible to obtain a high concentration extract of low molecular amino acids and peptide amino acids by the usual extraction method. As a result, it is highly possible to be applied as a basic agent for cosmetics, health foods, soft drinks, pharmaceuticals, and the like, and can also be used for food additives, pet food, various feeds, fertilizers, and the like.

Code based on [FIG. 1] (1) DPPH (1-1-Dipheyl-2-picryLhydeazil, λmax = 517 nm)
Free radical (2) H. (Excited active hydrogen)
(3) DPPH-H Molecular structural formula in which DP · H is generated by capturing H · at the N part of the DPPH free radical.
(A) ESRDPPH Signal (h1)
(B) ESRDPPH-H Signal (h2)
(H2 / h1 <1): ESR Signal intensity (I)
(Signal intensity (I) is 1 or less due to H-trapping.)

Claims (4)

  After immersing and impregnating pineapple enzyme juice containing natural mineral ions, stems, roots, leaves, and seeds of Moringa belonging to the Wasabiaceae family are obtained by pulverization after superheated steam drying sterilization. Moringa dry sterilized powder and moringa extract liquid extracted from dry sterilized powder and low molecular weight vegetable oil obtained by press extraction of seeds.   Natural mineral ion-containing pineapple enzyme juice is obtained by immersing natural ore hillstone, barley stone, medite stone, zeolite alone or multiple in pineapple peel juice, calcium, sodium, magnesium, potassium, iron, aluminum, zinc, A sterilized moringa stem / root / leaf / seed sterilized moringa powder and a moringa extract extracted from the sterilized sterilized powder containing seeds of mineral ions such as silicon (claim 1) Low molecular weight vegetable oil by compression extraction   Moringa stems, roots, leaves, and seeds belonging to the family Wasabiaceae are soaked and impregnated in pineapple enzyme juice containing natural mineral ions before sterilization with superheated steam to prevent oxidative decay and to prevent the generation of odors and to soften useful ingredients. By dry sterilization powder of moringa stems, roots, leaves and seeds belonging to Wasabiaceae as described in (Claim 1) and (Claim 2), and by moringa extract extracted from dry sterilization powder and by press extraction of seeds Low molecular vegetable oil.   Histidine, lysine, tryptophan, methionine, phenylalanine, threonine, leucine, isoleucine, valine as essential amino acids of low molecular weight amino acids as useful components of Moringa belonging to the family Wasabiaceae. Non-essential amino acids such as aspartic acid, serine, glutamic acid, proline, glycine, alanine, tyrosine, arginine, β-aminobutyric acid, γ-aminobutyric acid GABA and protein, lipid, carbohydrate, dietary fiber, calcium, magnesium, phosphorus, potassium, zinc [Claim 1] [Claim 2] [Claim 3] Ingredients containing copper, iron, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin C, vitamin E and the like are easily extracted by liquid. Moringa stalks, roots, leaves and seeds of Moringa belonging to the Wasabiaceae family, Moringa extract extracted from dried sterilized powders and low molecular weight vegetable oils by squeezing the seeds.

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