JP2011116591A - Apparatus for producing organic polymer crystal - Google Patents

Abstract

PROBLEM TO BE SOLVED: To store an organic polymer solution and a crystallization inducing liquid separately from each other, and to generate an organic polymer in the organic polymer solution by vapor diffusion, the concentration of the solvent in the conventional organic polymer crystal apparatus by vapor diffusion Conditions were difficult to set, and skill was required to handle the equipment.
An organic polymer crystallization apparatus is provided by suspending a holder in which a permeable membrane is attached to a permeable membrane attaching hole to a crystallization inducing liquid reservoir capable of storing a crystallization inducing liquid. By storing the gel body in which the organic polymer crystal is dissolved in the holder and using the organic polymer crystal apparatus according to the present invention, the organic polymer crystal can be easily generated in the gel body. The crystal after the production can be handled easily.
[Selection] Figure 7

Description

  The present invention relates to an apparatus for producing an organic polymer crystal, and more particularly to an apparatus for producing a crystal of biological polymer such as protein, nucleic acid, peptide and the like.

  Obtaining organic polymer crystals including proteins (hereinafter referred to as organic polymer crystals) is an important technology that enables analysis of the three-dimensional structure of organic polymers by X-ray diffraction, etc. It greatly contributes to.

  Conventionally, in order to produce an organic polymer crystal, an organic polymer crystal production container 101 having storage chambers 102 and 103 separated from each other with a partition wall 104 as shown in FIG. The organic polymer solution 105 and the crystallization inducing liquid 106 are stored separately from each other, and the solvent in the organic polymer solution 105 vapor diffuses into the crystallization inducing liquid 106 through the space 107, thereby An apparatus for generating (crystallizing) an organic polymer crystal in the polymer solution 105 has been used.

JP 2004-307335 A

  However, when the above apparatus is used, organic polymer crystals, in particular, crystals derived from living organisms such as proteins, nucleic acids, peptides, etc., are brittle to physical impacts, and when a seed crystal is placed in an organic polymer solution and manufactured. When moving the organic polymer crystal, there is a risk of direct contact with the crystal and damage.

  Further, when crystallization is performed in an organic polymer solution, the seed crystal is dissolved due to a change in the concentration of the solvent around the seed crystal and the osmotic pressure, and crystallization may not occur.

  Therefore, in the conventional apparatus for producing organic polymer crystals in a solution, even if the production apparatus is provided to a new drug developer, the work is complicated, and it is necessary to acquire skilled techniques to master the production apparatus. There was a problem.

  In addition, since the present invention is an apparatus for producing organic polymer crystals, particularly crystals of biologically derived polymers such as proteins, nucleic acids, peptides, etc., the organic polymer used as a raw material is expensive, and a large amount of organic It is often difficult to prepare a polymer solution. Therefore, it was necessary to develop a minute manufacturing apparatus that can be crystallized using a small amount of an organic polymer solution.

  Therefore, in order to solve the above problems, the present invention is an organic polymer crystal manufacturing apparatus used for manufacturing an organic polymer crystal, which has an open end at the upper end, is formed into a bottomed cylindrical mold, A crystallization-inducing liquid storage device provided with a storage space for storing the crystallization-inducing liquid, a container portion having an open end that opens upward and bulging downward from the open end and having a storage space therein, and A holder provided with a flat plate portion extending in the horizontal direction from the outer peripheral edge of the open end, and a permeable membrane attachment hole is formed in the container portion, covering the permeable membrane attachment hole, A permeable membrane having a hole is affixed, and the holder locks the flat plate portion to the opening end of the crystallization-inducing liquid reservoir, so that the lower part of the container part fits into the crystallization-inducing liquid reservoir, Suspend the permeable membrane immersed in the crystallization-inducing liquid in the crystallization-inducing liquid reservoir. Is characterized in that the gel body is accommodated in the accommodating space of the casing portion.

  The crystallization-inducing liquid reservoir is preferably a plastic plastic container. The resin that forms the crystallization-inducing liquid reservoir is not particularly limited, but one selected from polyethylene terephthalate (PET), polystyrene, and polycarbonate (PC) is preferably used, and PET is particularly preferable. This is because PET is a resin suitable for injection molding and has excellent transparency, so that the amount of the crystallization-inducing liquid stored in the crystallization-inducing liquid reservoir can be easily visually confirmed.

  The crystallization inducing liquid stored in the crystallization inducing liquid reservoir is stored in a storage space in the crystallization inducing liquid reservoir and is used for the purpose of generating organic polymer crystals. A solution in which a crystallization inducer is dissolved in a solvent is used. Examples of the crystallization inducer include sodium chloride, calcium chloride, sodium acetate, ammonium acetate, ammonium phosphate, ammonium sulfate, potassium sodium tartrate, sodium citrate, PEG (polyethylene glycol), magnesium chloride, sodium cacodylate, HEPES ( 2- [4- (2-hydroxyethyl) -1-piperazinyl] ethanesulfonic acid), MPD (2-methyl-2,4-pentanediol), Tris-HCl (trishydroxymethylaminomethane hydrochloride) At least one selected can be used.

  Solvents for dissolving the crystallization inducer include, for example, water, ethanol, methanol, acetonitrile, acetone, anisole, isopropanol, ethyl acetate, butyl acetate, chloroform, cyclohexane, diethylamine, dimethylacetamide, dimethylformamide, toluene, butanol, At least one selected from the group consisting of butyl methyl ether, hexane, benzene, and methyl ethyl ketone can be used, and water is preferred.

  The concentration of the crystallization-inducing liquid is not particularly limited, but is, for example, 0.0001 to 10.0M, preferably 0.0005 to 8.0M, more preferably 0.0005 to 6.0M. Moreover, the said crystallization induction liquid may contain the pH adjuster etc. suitably as needed.

  The holder is preferably formed of a transparent resin plastic. Although it does not restrict | limit especially as resin which forms a holder, It is preferable to use one selected from polyethylene terephthalate (PET), polystyrene, and polycarbonate (PC), and PET is especially preferable. Since PET is a resin suitable for injection molding and has excellent transparency, the organic polymer crystals generated in the holder are observed from above with a microscope by molding the holder transparently with PET. This makes it easy to check the quality of the crystal.

  It is preferable that the flat plate portion formed to extend in the horizontal direction from the outer peripheral edge of the open end of the holder is formed on the entire periphery of the outer peripheral edge of the open end. It is because it becomes stable when the holder is suspended from the crystallization-inducing liquid reservoir. Further, the flat plate portion may be formed on a part of the outer peripheral edge. In this case, the flat plate portion is in the height direction of the container portion for the purpose of stably suspending the holder from the crystallization-inducing liquid reservoir. Preferably, a pair of or more are formed with the axis of symmetry as the center of symmetry.

  The permeable membrane sticking hole formed in the container part is formed through the container part. It is preferable that the permeable membrane sticking hole is formed in any one of the bottom plate or side wall which comprises a container part, and may be formed in both the bottom plate and the side wall. However, the bottom plate and the side wall constituting the container portion do not need to be configured to be clearly distinguishable. For example, the bottom plate of the container portion may be formed in a hemispherical shape and smoothly connected to the side wall.

  In particular, the permeable membrane attachment hole is preferably formed in the bottom plate of the container. As a result, when the organic polymer solution is dropped onto the gel body accommodated in the container portion and the centrifugal operation is performed with the bottom plate in the centrifugal direction in order to dissolve the organic polymer solution in the gel body, the gel is A certain amount of moisture contained from the beginning in the body is discharged through the permeable membrane attachment hole and the permeable membrane formed in the bottom plate. Therefore, the organic polymer solution can be efficiently dissolved in the gel body by replacing the discharged contained moisture.

  Further, since the permeable membrane attaching hole is formed in the bottom plate of the container part, the generated organic polymer crystal can be taken out through the bottom plate by peeling off the permeable membrane attached to the permeable membrane attaching hole. This is advantageous when an organic polymer crystal is generated near the bottom plate.

  Furthermore, it is preferable that the permeable membrane attachment hole is formed on the side wall of the container. When the permeable membrane attachment hole is formed on the side wall of the container part, it is easier to increase the area of the permeable membrane attachment hole than when the permeable membrane attachment hole is formed on the bottom plate. The production efficiency of the organic polymer crystal can be improved by increasing the penetration rate into the inside and increasing the crystallization speed of the organic polymer crystal.

  In addition, the permeable membrane sticking hole can be formed in the shape of a circle or a slit, and the permeable membrane sticking hole to be formed may be one or plural.

  In addition, since the present invention is an apparatus for producing organic polymer crystals, particularly crystals of biologically derived polymers such as proteins, nucleic acids, peptides, etc., the organic polymer used as a raw material is expensive, and a large amount of organic polymers Since it is difficult to secure a molecular solution, a holding body having a large capacity of a storage space in which a gel body is stored is inconvenient.

  On the other hand, the size of the organic polymer crystal to be produced in the present invention is about 0.1 to 1.0 mm, and the crystal can be produced using a small amount of organic polymer solution. For this purpose, it is convenient to use a holding body with a small capacity of the accommodation space. However, if the holding body is miniaturized, the permeable membrane affixing hole formed in the container part is also miniaturized. The problem that it becomes difficult to paste is generated.

  Therefore, it is preferable that the permeable membrane to be attached to cover the permeable membrane attachment hole is attached by ultrasonic welding. By affixing by ultrasonic welding, a permeable membrane can be firmly affixed to a permeable membrane affixing hole having an opening width of about 1.0 to 4.0 mm. As the ultrasonic waves used for the ultrasonic welding of the permeable membrane attaching hole, longitudinal vibration ultrasonic welding that vibrates in a direction perpendicular to the outer surface of the peripheral portion of the permeable membrane attaching hole can be used.

  Also, the torsional vibration ultrasonic welding is performed in which the permeable membrane is welded while conducting a torsional ultrasonic wave that reciprocally vibrates in the circumferential direction of the permeable membrane attaching hole while pressing and attaching the permeable membrane to the peripheral portion of the permeable membrane attaching hole It is preferable that it is what was affixed. By using torsional vibration ultrasonic welding, the permeable membrane can be more firmly attached to the permeable membrane attachment hole having an opening width of about 1.0 to 3.0 mm. Therefore, even when the organic polymer solution is dropped on the gel body accommodated in the container part of the holder and the centrifugal operation is performed to dissolve the organic polymer solution in the gel body, the permeable membrane is not easily peeled off. There is an advantage.

  In the case of using ultrasonic waves, it is preferable that a plurality of minute protrusions be formed in advance along the periphery of the permeable membrane attachment hole on the outer surface of the periphery of the permeable membrane attachment hole to which the permeable membrane is attached. . This is because the adhesion of the permeable membrane can be improved by applying ultrasonic waves by applying pressure to the permeable membrane on the outer surface of the peripheral edge of the permeable membrane attachment hole in which the minute protrusions are formed. In particular, it is effective for obtaining sufficient adhesion of the permeable membrane by longitudinal vibration ultrasonic welding.

  The permeable membrane is preferably made of resin. Although it does not restrict | limit especially as resin which forms a permeable film, It is preferable to use one selected from polyethylene terephthalate (PET), polystyrene, and polycarbonate (PC). Resin different from resin which comprises a permeable membrane and a holder can also be used, and the same resin can also be used. In particular, when the same resin as the resin constituting the holder is used, it is easier to firmly attach the permeable membrane to the permeable membrane attachment hole than when using a different resin. There is an advantage that the permeable membrane is difficult to peel off even when an organic polymer solution is dropped onto the accommodated gel body and a centrifugal operation is performed to dissolve the organic polymer solution in the gel body. Therefore, for example, when the holder is formed of PET, it is preferable that the permeable membrane is also formed of PET.

The average diameter of the micropores formed in the permeable membrane is preferably 0.1 to 0.8 micrometers, and more preferably 0.3 to 0.6 micrometers. The density of the micropores formed in the transparent film is preferably a 0.0000001～0.00001Cm ^-2, and more preferably 0.000001~0.000003cm ^-2. In order to obtain high-quality crystals, it is preferable that the penetration rate of the crystallization-inducing liquid penetrating into the gel body is lower than the rate of crystallization of the organic polymer. This is because it is easy to obtain the penetration rate of the crystallization-inducing liquid.

  In addition, the gel body used in order to produce | generate an organic polymer crystal is accommodated in a container part. The gel body is accommodated in an accommodation space provided inside the side wall and the bottom wall of the container portion and below the open end. Further, the gel body is preferably at least one selected from the group consisting of polysaccharides, thickening polysaccharides, proteins, and gels at elevated temperature, agarose, agar, carrageenan, gelatin, collagen, More preferably, it is at least one selected from the group consisting of polyacrylamide and gelled polyacrylamide gel at elevated temperature.

  The holder is suspended from the crystallization-inducing liquid reservoir in which the crystallization-inducing liquid is stored, and the container part is fitted in the crystallization-inducing storage part. At this time, at least the permeable membrane is immersed in the crystallization inducing liquid in the container part of the holder.

  Then, the crystallization inducing liquid permeates through the permeable membrane and gradually permeates into the gel accommodated in the holder, and gradually decreases the supersaturation degree of the organic polymer dissolved in the gel body in advance.

  As a result, the organic polymer dissolved in the gel body is slowly crystallized, and an organic polymer crystal can be generated in the gel body.

  According to the present invention, an organic polymer crystal conjugated to a gel body can be produced.

  Since the organic polymer crystal conjugated in the gel body can be moved together with the surrounding gel body, even a crystal derived from a living body such as protein, nucleic acid, peptide, etc. can be moved without being damaged.

  In addition, by using the present invention, the organic polymer crystal can be generated in a stable environment in which the surrounding environment in the gel body hardly flows. There is no problem that the seed crystal dissolves due to a change in osmotic pressure, and an organic polymer crystal can be easily produced even by a user unfamiliar with the operation of the apparatus according to the present invention.

  Furthermore, after the organic polymer crystal is formed, the holder and the crystallization-inducing liquid reservoir can be handled separately, so that the organic polymer crystal can be used when observing and taking out the organic polymer crystal. When handling the crystallization inducing liquid, it is possible to prevent the crystallization inducing liquid from leaking out of the crystallization inducing liquid reservoir.

It is the perspective view which looked at the holder 2 from the lower part. FIG. 4 is a longitudinal sectional view of the state in which the holder 2 is suspended from the crystallization-inducing liquid reservoir 3 cut at the center of the holder 2. FIG. 4 is a longitudinal sectional view showing a state in which the gel body G1 is housed in the container portion 21 by cutting at the center of the holder 2; FIG. 4 is a longitudinal sectional view showing a state in which an organic polymer solution P is dropped on the gel body G1 in the container part 21 by cutting at the center of the holder 2. FIG. 4 is a cross-sectional view showing a state of the gel body G2 in the container part 21 cut at the center of the holder 2. It is a longitudinal cross-sectional view which shows a mode that the organic polymer crystal manufacturing apparatus 1 was cut | disconnected in the center of the holder 2, and the holder 2 in which the gel body G2 was accommodated was suspended by the crystallization induction liquid storage tool 3. FIG. It is a longitudinal cross-sectional view which shows a mode that the organic polymer crystal manufacturing apparatus 1 was cut | disconnected in the center of the holder 2, and the organic polymer crystal C produced | generated in the gel body G2. FIG. 3 is a longitudinal sectional view showing a state in which an organic polymer crystal C is generated in an organic polymer solution P by cutting the organic polymer crystal production apparatus 1 at the center of a holder 2. It is a figure which shows the outline of a structure of the organic polymer crystal manufacturing apparatus 1 at the time of combining the holder 2 which has the some container part 21, and the some crystallization induction liquid storage tool 3. FIG. It is the longitudinal cross-sectional view which cut | disconnected the conventional organic polymer crystal production | generation container 101 in the center of the storage chamber 102 and the storage chamber 103. FIG.

  Hereinafter, embodiments according to the present invention will be described in detail with reference to the drawings.

  FIG. 1 is a perspective view of the holder 2 and the permeable membrane 4 attached to the holder 2 when viewed obliquely from below.

The holder 2 is formed by reducing the diameter of the side peripheral wall 211 downward, and forming a truncated cone-shaped container part 21 in which a circular permeable membrane application hole 213 is formed in the bottom plate 212 and an upper end surface of the container part 21 upward. The open end 22 is formed by being opened, and the flat plate portion 23 is formed by extending horizontally from the outer peripheral edge of the open end 22 outward in the circumferential direction. A storage space 24 in which the gel body G is stored is provided inside the side peripheral wall 211 and the bottom plate 212 and below the open end 22.
The shape of the container portion 21 may be a cylindrical shape or a rectangular tube shape.

  The permeable membrane 4 is attached to the outer surface of the bottom plate 212 and covers the entire permeable membrane attaching hole 14.

  In this embodiment, the holder 2 is integrally formed by an injection molding method using a transparent PET resin as a raw material.

  As shown in FIG. 1, the holder 2 may have a plurality of container portions 21 formed in a lattice shape on a flat plate portion 23 formed integrally. By providing a plurality of container parts 21, the organic polymer crystal C can be mass-produced and various organic polymer crystals C can be produced simultaneously.

  After the holder 2 is molded, the ultrasonic wave generated by torsional vibration is transmitted to the bottom plate 212 and the permeable membrane 4 while pressing the permeable membrane 4 against the outer surface of the bottom plate 212 at the peripheral portion of the permeable membrane attaching hole 213. By sonic welding, the permeable membrane 4 was pasted so as to cover the permeable membrane pasting hole 213.

  In this example, the diameter of the permeable membrane bonding hole 213 formed in the bottom plate 212 was formed to be 2.0 to 3.0 mm.

  The permeable membrane 4 attached in this example was formed from a PET resin.

  The crystallization-inducing liquid reservoir 3 is formed into a bottomed cylindrical shape, and has a storage space 31 in which the crystallization-inducing liquid R can be stored inside the cylinder. The inner diameter of the formed open end 32 is slightly larger than the outer diameter of the portion where the container portion 21 is joined to the flat plate portion 23.

  The crystallization inducing liquid reservoir 3 is integrally formed by an injection molding method using a transparent PET resin as a raw material.

  Further, the crystallization inducing liquid reservoir 3 may be integrally formed so that the plurality of crystallization inducing liquid reservoirs 3 are arranged in a lattice pattern. In this way, the plurality of crystallization-inducing liquid reservoirs 3 are integrally formed, so that the organic polymer crystal C can be mass-produced in combination with the holder 2 having the plurality of container parts 21, and various kinds of organic high crystals can be produced. Molecular crystal C can be produced simultaneously.

  As shown in FIG. 2, the organic polymer crystal production apparatus 1 is configured by placing the holder 2 to which the permeable membrane 4 is attached on a crystallization induction liquid reservoir 3. At this time, the flat plate portion 23 is locked to the open end 32 of the crystallization-inducing liquid reservoir 3 and is suspended so that the lower portion of the container 12 is fitted into the crystallization-inducing liquid reservoir 3.

  The procedure 1 for producing the organic polymer crystal C using the present invention will be described below.

  First, as shown in FIG. 3, the gel body G1 is accommodated in the accommodation space 24 provided in the container portion 21 of the holder 2 to which the permeable membrane 4 is attached. In this example, agarose was used for the gel body G1.

  Next, as shown in FIG. 4, an organic polymer solution P in which an organic polymer such as a protein is dissolved is dropped onto the gel body G1, and the open end 22 of the container portion 21 is sealed with the lid body 5. 2 was installed in a centrifuge, and a centrifugal operation was performed in a direction in which the organic polymer solution P pressed the gel body G1. By this centrifugal operation, the organic polymer solution P can be infiltrated into the gel body G1, and the gel body G2 in which the organic polymer is dissolved in the gel body G1 can be obtained as shown in FIG.

  The lid 5 can be a thin glass plate, and is formed of a flexible resin thin film, and an adhesive having a weak adhesive force that can be attached to and detached from the open end 22 is formed on the surface in contact with the open end 22. Even if it is provided, it is more preferable. Moreover, in order to prevent that the water | moisture content contained in the gel body G1 evaporates during producing | generating an organic polymer crystal, it is preferable that the cover body 5 has a property which cannot permeate | transmit water vapor | steam.

  Here, in this example, since the circular permeable membrane sticking hole 213 was formed in the bottom plate 212 of the container portion 21 and the permeable membrane 4 was stuck, the gel body G1 was contained by the centrifugal operation. A certain amount of moisture can be discharged from the permeable membrane 4. As a result, the organic polymer solution P is easily permeated into the gel body G1 by replacing the water discharged from the gel body G1, so that, for example, compared with the case where the permeable membrane bonding hole 213 is formed in the peripheral side wall 211. Thus, there is an advantage that the organic polymer can be easily dissolved in the gel body G1 by a shorter time centrifugation operation.

  Subsequently, as shown in FIG. 6, the holder 2 is suspended from the crystallization inducing liquid reservoir 3 in which the crystallization inducing liquid R is stored. In this embodiment, a sodium chloride aqueous solution having a concentration of 1.0 M was used as the crystallization inducing liquid R.

  The holder 2 suspended from the crystal inducing liquid reservoir 3 has the lower part of the container 21 immersed in the crystal inducing liquid R, and the crystal inducing liquid R gradually permeates into the gel body G2 through the permeable membrane 4. The crystal inducing liquid R that has permeated into the gel body G2 gradually decreases the supersaturation degree of the organic polymer dissolved in the gel body G2.

  As a result, as shown in FIG. 7, the organic polymer dissolved in the gel body G2 is slowly crystallized, and the organic polymer crystal C can be generated in the gel body G2.

  After the quality of the produced organic polymer crystal C is confirmed by microscopic observation, the gel body G2 and the gel body G2 are taken out from the container part 21, and the manufacturing process of the organic polymer crystal is completed.

  Here, when a plurality of crystallization-inducing liquid reservoirs 3 are integrally formed, as shown in FIG. 9, at least all of the crystallization-inducing liquid reservoirs 3 correspond one-to-one to each of the plurality of container parts 21. It is preferable to be able to be molded.

  In addition, according to this invention which concerns on a present Example, it becomes easy to screen the crystallization conditions of the organic polymer crystal C. FIG. That is, since the crystal induction liquid R gradually permeates from below into the gel body G2 in which the organic polymer is dissolved, it is determined at which position in the gel body G2 the organic polymer crystal C is generated. Thus, the optimum concentration ratio (mixing ratio) between the organic polymer and the crystallization-inducing liquid R in the gel body G2 for generating the organic polymer crystal C can be easily obtained.

  Accordingly, when the organic polymer crystal production container 101 as shown in FIG. 10 is used, only one concentration condition can be examined in one container, and a plurality of containers are necessary to determine the optimum condition. On the other hand, according to the present invention, it becomes possible to easily screen in one container part 21 that has been difficult in the past, and it is possible to easily know the crystallization conditions such as the concentration ratios that differ for each type of organic polymer. There is an advantage that can be.

  According to the present invention, since the gel body G1 accommodated in the container portion 21 is solid, the holder 2 in which the gel body G1 is previously stored in the container portion 21 can be delivered to the user. It is. Thereby, the production | generation of the organic polymer crystal | crystallization C can be performed, without a user taking the effort which accommodates the gel body G1.

  Next, procedure 2 for producing the organic polymer crystal C using the present invention will be described below.

  In the procedure 2, the gel body G1 is first accommodated in the accommodating space 24 provided in the container portion 21 of the holding portion 2 as in the procedure 1. Next, after dropping the organic polymer solution P onto the gel body G1, the open end 22 of the container part 21 is sealed with the lid 5 without performing the centrifugation operation performed in the procedure 1, and the crystallization inducing liquid R is sealed. The holder 2 is suspended from the crystallization-inducing liquid reservoir 3 storing the above and left standing.

  Then, the crystallization inducing liquid R gradually permeates the gel body G1 and reaches the organic polymer solution P, and gradually lowers the degree of supersaturation of the organic polymer dissolved in the organic polymer solution P.

  As a result, as shown in FIG. 8, the organic polymer dissolved in the organic polymer solution P is slowly crystallized, and the organic polymer crystal C can be generated in the organic polymer solution P.

  After the quality of the produced organic polymer crystal C is confirmed by microscopic observation, the organic polymer crystal C is carefully taken out from the organic polymer solution P so as not to damage the crystal, and the manufacturing process of the organic polymer crystal is completed.

  When the organic polymer crystal C is generated by the procedure 2, since the organic polymer crystal C is generated in the organic polymer solution P, the organic polymer crystal C cannot be conjugated to the gel body. Can be omitted.

  Further, since the crystallization-inducing liquid R reaches the organic polymer solution P after permeating through the gel body G1, it does not cause a sudden decrease in supersaturation even in crystallization in the solution, and is good. Organic polymer crystals C can be produced.

DESCRIPTION OF SYMBOLS 1 Organic polymer crystal production apparatus 2 Holder 21 Container part 211 Side peripheral wall 212 Bottom plate 213 Permeation membrane sticking hole 22 Open end 23 Flat plate part 24 Storage space 3 Crystallization inducing liquid reservoir 31 Storage space 32 Open end 4 Permeation membrane 5 Lid Body G1 gel body R crystallization inducing liquid

Claims (4)

An organic polymer crystal manufacturing apparatus used for manufacturing an organic polymer crystal,
A crystallization-inducing liquid reservoir having an open end at the upper end, formed into a bottomed cylindrical mold, and provided with a storage space for storing the crystallization-inducing liquid therein,
A holding portion having an open end that opens upward, a container portion that bulges downward from the open end and has a storage space therein, and a flat plate portion that extends horizontally from the outer peripheral edge of the open end. Made of ingredients,
A permeable membrane attachment hole is formed in the container part,
Covering the permeable membrane attachment hole, a permeable membrane having a large number of micropores is attached,
The holder locks the flat plate portion to the opening end of the crystallization-inducing liquid reservoir, so that the lower part of the container portion fits into the crystallization-inducing liquid reservoir, and the permeable membrane is the crystallization-inducing liquid reservoir. Suspended in the crystallization-inducing liquid inside,
An organic polymer crystal manufacturing apparatus, wherein a gel body is accommodated in an accommodation space of the container part. 2. The organic polymer crystal production apparatus according to claim 1, wherein the permeation membrane attaching hole is formed in a bottom plate of the container part. The organic polymer crystal production apparatus according to claim 1, wherein the permeable film is attached by ultrasonic welding. The permeable membrane is affixed by torsional vibration ultrasonic welding that conducts and welds torsional ultrasonic waves that reciprocally vibrate in the circumferential direction of the permeable membrane attaching hole while pressing and attaching the permeable membrane to the peripheral portion of the permeable membrane attaching hole. The organic polymer crystal production apparatus according to claim 1 or 2, wherein

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