JP2010115122A5 - - Google Patents
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- JP2010115122A5 JP2010115122A5 JP2008288638A JP2008288638A JP2010115122A5 JP 2010115122 A5 JP2010115122 A5 JP 2010115122A5 JP 2008288638 A JP2008288638 A JP 2008288638A JP 2008288638 A JP2008288638 A JP 2008288638A JP 2010115122 A5 JP2010115122 A5 JP 2010115122A5
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- 108091034117 Oligonucleotide Proteins 0.000 claims 65
- 238000001514 detection method Methods 0.000 claims 24
- 241000228212 Aspergillus Species 0.000 claims 22
- 230000000295 complement effect Effects 0.000 claims 20
- 241001225321 Aspergillus fumigatus Species 0.000 claims 16
- 241000894006 Bacteria Species 0.000 claims 16
- 229940091771 aspergillus fumigatus Drugs 0.000 claims 16
- 239000002773 nucleotide Substances 0.000 claims 16
- 125000003729 nucleotide group Chemical group 0.000 claims 16
- 108020004707 nucleic acids Proteins 0.000 claims 14
- 150000007523 nucleic acids Chemical class 0.000 claims 14
- 102000039446 nucleic acids Human genes 0.000 claims 14
- 241000306560 Aspergillus udagawae Species 0.000 claims 12
- 241001149711 Aspergillus lentulus Species 0.000 claims 9
- 108090000704 Tubulin Proteins 0.000 claims 9
- 230000004544 DNA amplification Effects 0.000 claims 8
- 241000824759 Aspergillus fumisynnematus Species 0.000 claims 7
- 238000000034 method Methods 0.000 claims 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 5
- 230000003321 amplification Effects 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 4
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 claims 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 claims 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 239000002853 nucleic acid probe Substances 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
Claims (30)
（ａ）配列番号１若しくは２に記載のβ−チューブリン遺伝子の部分塩基配列、又はその相補配列
（ｂ）配列番号１若しくは２に記載の塩基配列において１若しくは数個の塩基が欠失、置換若しくは付加された塩基配列、又はその相補配列 A method for detecting an Aspergillus fumigatus ( Aspergillus fumigatus ) -related bacterium characterized by identifying an Aspergillus fumigatus ( Aspergillus fumigatus ) -related bacterium using a nucleic acid represented by the base sequence described in (a) or (b) below .
(A) Partial base sequence of β-tubulin gene described in SEQ ID NO: 1 or 2 or its complementary sequence (b) One or several bases are deleted or replaced in the base sequence described in SEQ ID NO: 1 or 2 or added in the nucleotide sequence, or its complementary sequence
（ａ−１）配列番号１に記載のβ−チューブリン遺伝子の部分塩基配列、若しくはその相補配列
（ｂ−１）配列番号１に記載の塩基配列において１若しくは数個の塩基が欠失、置換若しくは付加された塩基配列、若しくはその相補配列 The Aspergillus fumigatus-related bacterium is Aspergillus lentulus and / or Aspergillus fumicinetus ( Aspergillus fumignanematus ), which is represented by the base sequence described in the following (a-1) or (b-1) The detection method according to claim 2 or 3, wherein the region is subjected to gene amplification and the presence or absence of a gene amplification product is confirmed.
(A-1) Partial base sequence of β-tubulin gene described in SEQ ID NO: 1 or its complementary sequence (b-1) One or several bases deleted or substituted in the base sequence described in SEQ ID NO: 1 Or the added base sequence or its complementary sequence
（ａ−２）配列番号２に記載のβ−チューブリン遺伝子の部分塩基配列、若しくはその相補配列
（ｂ−２）配列番号２に記載の塩基配列において１若しくは数個の塩基が欠失、置換若しくは付加された塩基配列、若しくはその相補配列 The Aspergillus fumigatus related bacteria an Aspergillus Udagawae (Aspergillus udagawae), the following areas in the nucleic acid represented by the nucleotide sequence set forth in (a-2) or (b-2) gene amplification, gene amplification products 4. The detection method according to claim 2 or 3, wherein the presence or absence of the above is confirmed.
(A-2) Partial base sequence of the β-tubulin gene described in SEQ ID NO: 2 or its complementary sequence (b-2) One or several bases are deleted or replaced in the base sequence described in SEQ ID NO: 2 Or the added base sequence or its complementary sequence
（ｃ）配列番号３に記載の塩基配列で表されるオリゴヌクレオチド、又は当該塩基配列に対して７０％以上の相同性を有しかつプライマーとして使用できる塩基配列で表されるオリゴヌクレオチド
（ｄ）配列番号４に記載の塩基配列で表されるオリゴヌクレオチド、又は当該塩基配列に対して７０％以上の相同性を有しかつプライマーとして使用できる塩基配列で表されるオリゴヌクレオチド The following oligonucleotides (c) and (d) perform an amplification reaction using as a nucleic acid primer, Aspergillus lentus (Aspergillus lentulus) and / or Aspergillus Fumishinematasu (Aspergillus fumisynnematus) according to claim 6, wherein the detecting the Detection method.
(C) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 3 or an oligonucleotide represented by a base sequence having 70% or more homology to the base sequence and usable as a primer (d) Oligonucleotide represented by the nucleotide sequence set forth in SEQ ID NO: 4, or oligonucleotide represented by a nucleotide sequence having 70% or more homology with the nucleotide sequence and usable as a primer
（ｅ）配列番号５に記載の塩基配列で表されるオリゴヌクレオチド、又は当該塩基配列に対して７０％以上の相同性を有しかつプライマーとして使用できる塩基配列で表されるオリゴヌクレオチド
（ｆ）配列番号６に記載の塩基配列で表されるオリゴヌクレオチド、又は当該塩基配列に対して７０％以上の相同性を有しかつプライマーとして使用できる塩基配列で表されるオリゴヌクレオチド The detection method according to claim 6 , wherein an amplification reaction is carried out using the following oligonucleotides (e) and (f) as nucleic acid primers to detect Aspergillus udagawae .
(E) the oligonucleotide represented by the base sequence set forth in SEQ ID NO: 5 or the oligonucleotide represented by the base sequence that has 70% or more homology to the base sequence and can be used as a primer (f) Oligonucleotide represented by the nucleotide sequence set forth in SEQ ID NO: 6, or oligonucleotide represented by a nucleotide sequence having 70% or more homology with the nucleotide sequence and usable as a primer
（ｇ）配列番号７に記載の塩基配列で表されるオリゴヌクレオチド
（ｈ）配列番号８に記載の塩基配列で表されるオリゴヌクレオチド
（ｉ）配列番号９に記載の塩基配列で表されるオリゴヌクレオチド
（ｊ）配列番号１０に記載の塩基配列で表されるオリゴヌクレオチド The oligonucleotide sets the following (g) ~ (j) carried out an amplification reaction using as a nucleic acid primer according to claim 9, wherein the detecting Aspergillus lentus (Aspergillus lentulus) and / or Aspergillus Fumishinematasu (Aspergillus fumisynnematus) Detection method.
(G) Oligonucleotide represented by the base sequence set forth in SEQ ID NO: 7 (h) Oligonucleotide represented by the base sequence set forth in SEQ ID NO: 8 (i) Oligonucleotide represented by the base sequence set forth in SEQ ID NO: 9 Nucleotide (j) Oligonucleotide represented by the nucleotide sequence set forth in SEQ ID NO: 10
（ｋ）配列番号１１に記載の塩基配列で表されるオリゴヌクレオチド
（ｌ）配列番号１２に記載の塩基配列で表されるオリゴヌクレオチド A claim 1 0 detection method according further detection method of claim 1 0, wherein using oligonucleotide as a nucleic acid primer below (k) and / or (l).
(K) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 11 (l) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 12
（ｍ）配列番号１３に記載の塩基配列で表されるオリゴヌクレオチド
（ｎ）配列番号１４に記載の塩基配列で表されるオリゴヌクレオチド
（ｏ）配列番号１５に記載の塩基配列で表されるオリゴヌクレオチド
（ｐ）配列番号１６に記載の塩基配列で表されるオリゴヌクレオチド The detection method according to claim 9 , wherein an amplification reaction is performed using the following oligonucleotide sets (m) to (p) as nucleic acid primers to detect Aspergillus udagawae .
(M) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 13 (n) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 14 (o) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 15 Nucleotide (p) Oligonucleotide represented by the nucleotide sequence set forth in SEQ ID NO: 16
（ｑ）配列番号１７に記載の塩基配列で表されるオリゴヌクレオチド
（ｒ）配列番号１８に記載の塩基配列で表されるオリゴヌクレオチド The detection process of claim 1 2 further detection method of claim 1 wherein using the oligonucleotide as a nucleic acid primer below (q) and / or (r).
(Q) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 17 (r) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 18
（ａ）配列番号１若しくは２に記載のβ−チューブリン遺伝子の部分塩基配列、又はその相補配列
（ｂ）配列番号１若しくは２に記載の塩基配列において１若しくは数個の塩基が欠失、置換若しくは付加された塩基配列、又はその相補配列 A DNA represented by the base sequence described in the following (a) or (b) for use in detecting an Aspergillus fumigatus- related bacterium.
(A) Partial base sequence of β-tubulin gene described in SEQ ID NO: 1 or 2 or its complementary sequence (b) One or several bases are deleted or replaced in the base sequence described in SEQ ID NO: 1 or 2 Or the added base sequence or its complementary sequence
（ａ−１）配列番号１に記載のβ−チューブリン遺伝子の部分塩基配列、若しくはその相補配列
（ｂ−１）配列番号１に記載の塩基配列において１若しくは数個の塩基が欠失、置換若しくは付加された塩基配列、若しくはその相補配列 Aspergillus lentus for use in the detection of (Aspergillus lentulus) and / or Aspergillus Fumishinematasu (Aspergillus fumisynnematus), DNA represented by the nucleotide sequence set forth in the following (a-1) or (b-1).
(A-1) Partial base sequence of β-tubulin gene described in SEQ ID NO: 1 or its complementary sequence (b-1) One or several bases deleted or substituted in the base sequence described in SEQ ID NO: 1 Or the added base sequence or its complementary sequence
（ａ−２）配列番号２に記載のβ−チューブリン遺伝子の部分塩基配列、若しくはその相補配列
（ｂ−２）配列番号２に記載の塩基配列において１若しくは数個の塩基が欠失、置換若しくは付加された塩基配列、若しくはその相補配列 A DNA represented by the base sequence described in the following (a-2) or (b-2) for use in detecting Aspergillus udagawae .
(A-2) Partial base sequence of the β-tubulin gene described in SEQ ID NO: 2 or its complementary sequence (b-2) One or several bases are deleted or replaced in the base sequence described in SEQ ID NO: 2 Or the added base sequence or its complementary sequence
（ａ）配列番号１若しくは２に記載のβ−チューブリン遺伝子の部分塩基配列、又はその相補配列
（ｂ）配列番号１若しくは２に記載の塩基配列において１若しくは数個の塩基が欠失、置換若しくは付加された塩基配列、又はその相補配列 It can hybridize to a nucleic acid represented by the base sequence described in (a) or (b) below, and functions as a nucleic acid probe or nucleic acid primer for specifically detecting an Aspergillus fumigatus- related bacterium. Obtained oligonucleotide for detecting Aspergillus fumigatus-related bacteria.
(A) Partial base sequence of β-tubulin gene described in SEQ ID NO: 1 or 2 or its complementary sequence (b) One or several bases are deleted or replaced in the base sequence described in SEQ ID NO: 1 or 2 Or the added base sequence or its complementary sequence
（ｃ）配列番号３に記載の塩基配列で表されるオリゴヌクレオチド、又は当該塩基配列に対して７０％以上の相同性を有しかつ検出用オリゴヌクレオチドとして使用できる塩基配列で表されるオリゴヌクレオチド
（ｄ）配列番号４に記載の塩基配列で表されるオリゴヌクレオチド、又は当該塩基配列に対して７０％以上の相同性を有しかつ検出用オリゴヌクレオチドとして使用できる塩基配列で表されるオリゴヌクレオチド Following (c) and (d) of Aspergillus lentus (Aspergillus lentulus) consisting of oligonucleotides and / or Aspergillus Fumishinematasu (Aspergillus fumisynnematus) detector oligonucleotide pair.
(C) the oligonucleotide represented by the base sequence set forth in SEQ ID NO: 3 or the oligonucleotide represented by the base sequence having 70% or more homology to the base sequence and usable as a detection oligonucleotide (D) the oligonucleotide represented by the base sequence set forth in SEQ ID NO: 4 or the oligonucleotide represented by the base sequence having 70% or more homology to the base sequence and usable as a detection oligonucleotide
（ｅ）配列番号５に記載の塩基配列で表されるオリゴヌクレオチド、又は当該塩基配列に対して７０％以上の相同性を有しかつ検出用オリゴヌクレオチドとして使用できる塩基配列で表されるオリゴヌクレオチド
（ｆ）配列番号６に記載の塩基配列で表されるオリゴヌクレオチド、又は当該塩基配列に対して７０％以上の相同性を有しかつ検出用オリゴヌクレオチドとして使用できる塩基配列で表されるオリゴヌクレオチド A pair of oligonucleotides for detection of Aspergillus udagawae comprising the following oligonucleotides (e) and (f):
(E) the oligonucleotide represented by the nucleotide sequence set forth in SEQ ID NO: 5 or the oligonucleotide represented by the nucleotide sequence having 70% or more homology with the nucleotide sequence and usable as a detection oligonucleotide (F) an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 6 or an oligonucleotide represented by a base sequence having 70% or more homology to the base sequence and usable as a detection oligonucleotide
（ｇ）配列番号７に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー
（ｈ）配列番号８に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー
（ｉ）配列番号９に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー
（ｊ）配列番号１０に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー Following (g) ~ consisting primer (j) Aspergillus lentus (Aspergillus lentulus) and / or Aspergillus Fumishinematasu (Aspergillus fumisynnematus) detection primer set.
(G) a primer composed of an oligonucleotide represented by the base sequence described in SEQ ID NO: 7 (h) a primer composed of an oligonucleotide represented by the base sequence described in SEQ ID NO: 8 (i) a base described in SEQ ID NO: 9 Primer comprising an oligonucleotide represented by the sequence (j) Primer comprising the oligonucleotide represented by the base sequence set forth in SEQ ID NO: 10
（ｋ）配列番号１１に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー
（ｌ）配列番号１２に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー Claim 2 5 primer set described further below (k) and / or claim 2 5, wherein the Aspergillus lentus, characterized in that it comprises a primer (l) (Aspergillus lentulus) and / or Aspergillus Fumishinematasu (Aspergillus fumisynnematus) Detection primer set.
(K) Primer comprising an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 11 (l) Primer comprising an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 12
（ｍ）配列番号１３に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー
（ｎ）配列番号１４に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー
（ｏ）配列番号１５に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー
（ｐ）配列番号１６に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー A primer set for detecting Aspergillus udagawae comprising the following primers (m) to (p):
(M) a primer composed of an oligonucleotide represented by the base sequence described in SEQ ID NO: 13 (n) a primer composed of an oligonucleotide represented by the base sequence described in SEQ ID NO: 14 (o) a base described in SEQ ID NO: 15 Primer comprising an oligonucleotide represented by the sequence (p) Primer comprising the oligonucleotide represented by the base sequence set forth in SEQ ID NO: 16
（ｑ）配列番号１７に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー
（ｒ）配列番号１８に記載の塩基配列で表されるオリゴヌクレオチドからなるプライマー Claim 2 7 claims 2 7 Aspergillus Udagawae (Aspergillus udagawae) detection primer set according primer set further characterized in that it comprises the following primers (q) and / or (r) according.
(Q) Primer consisting of an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 17 (r) Primer consisting of an oligonucleotide represented by the base sequence set forth in SEQ ID NO: 18
前記標的領域の３’側から順に、塩基配列領域としてＢ３ｃ、Ｂ２ｃ及びＢ１ｃを選択し、
前記Ｆ３、Ｆ２及びＦ１の相補的塩基配列を、それぞれＦ３ｃ、Ｆ２ｃ及びＦ１ｃとし、
前記Ｂ３ｃ、Ｂ２ｃ及びＢ１ｃに相補的な塩基配列を、それぞれＢ３、Ｂ２及びＢ１としたとき、
以下の（ａ）〜（ｆ）のいずれかに該当する塩基配列で表されるアスペルギルス フミガタス（Ａｓｐｅｒｇｉｌｌｕｓ ｆｕｍｉｇａｔｕｓ）類縁菌検出用オリゴヌクレオチド。
（ａ）前記Ｂ２領域を３’側に有し、前記Ｂ１ｃ領域を５’側に有する塩基配列
（ｂ）前記Ｂ３領域を有する塩基配列
（ｃ）前記Ｆ２領域を３’側に有し、前記Ｆ１ｃ領域を５’側に有する塩基配列
（ｄ）前記Ｆ３領域を有する塩基配列
（ｅ）前記Ｂ１領域と前記Ｂ２領域の間の部分と相補的な配列を有する塩基配列
（ｆ）前記Ｆ１領域と前記Ｆ２領域の間の部分と相補的な配列を有する塩基配列 F3, F2 and F1 are selected as base sequence regions in order from the 5 ′ side of the sequence of the target region selected from the base sequence of the β-tubulin gene of Aspergillus fumigatus- related bacteria.
In order from the 3 ′ side of the target region, B3c, B2c and B1c are selected as base sequence regions,
The complementary base sequences of F3, F2 and F1 are F3c, F2c and F1c, respectively.
When the base sequences complementary to B3c, B2c and B1c are B3, B2 and B1, respectively,
An oligonucleotide for detecting Aspergillus fumigatus- related bacteria represented by a base sequence corresponding to any of the following (a) to (f):
(A) a base sequence having the B2 region on the 3 ′ side and a B1c region on the 5 ′ side (b) a base sequence having the B3 region (c) having the F2 region on the 3 ′ side; A base sequence having an F1c region on the 5 ′ side (d) a base sequence having the F3 region (e) a base sequence having a sequence complementary to a portion between the B1 region and the B2 region (f) the F1 region A base sequence having a sequence complementary to a portion between the F2 regions
ＤＮＡポリメラーゼと、
ｄＡＴＰ、ｄＣＴＰ、ｄＧＴＰ及びｄＴＴＰを含むｄＮＴＰと、
を含むアスペルギルス フミガタス（Ａｓｐｅｒｇｉｌｌｕｓ ｆｕｍｉｇａｔｕｓ）類縁菌検出キット。
The claims 2 5, 2 6, 2 7 or 2 8 primer sets or claim 2 9, wherein the oligonucleotide according,
DNA polymerase;
dNTPs including dATP, dCTP, dGTP and dTTP;
Aspergillus fumigatus ( Aspergillus fumigatus ) -related bacteria detection kit.
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