JP2009234939A - Cosmetic composition containing vegetable origin polyamine-containing extract - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic composition having humectant effect, collagen production-accelerating effect, hyaluronic acid production-accelerating effect, cell-activating effect, anti-oxidation effect, anti-aging effect, hair-growing effect, skin roughness-improving effect, etc. <P>SOLUTION: This cosmetic composition is characterized by containing a vegetable origin polyamine-containing extract and a skin-whitening agent. The cosmetic composition can further contain an anti-aging agent, a humectant, an ultraviolet light absorber and an antibacterial agent. The cosmetic composition can be utilized as a cosmetic, quasi drug, medical material, hygienic material or medicine. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a cosmetic composition comprising a plant-derived polyamine-containing extract as an active ingredient, and is particularly used for cosmetics, quasi-drugs, medical products, hygiene products, and pharmaceuticals.

  Individual aging and the various diseases that accompany this are closely related to the aging of all dividing cells (decrease in division rate and cell function). For example, the skin is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that support these extracellular skin structures. In young skin, moisture retention, flexibility, and elasticity are ensured by maintaining the constancy of the interaction between these skin tissues, and the skin has a firm and glossy appearance and is kept fresh. However, aging, ultraviolet rays, drying, stress, etc. cause deterioration of the function of the extracellular matrix and fibroblasts in particular. As a result, the skin's flexibility and moisturizing function are reduced, and the skin loses its tension and gloss and becomes rough. Aging symptoms such as wrinkles and dullness occur.

  Searches for cell activators and anti-aging agents have been made for the purpose of preventing and suppressing aging at the cellular level. For example, connective tissue hydrolysates (Patent Document 1), thymus / spleen-derived water-soluble protein (Patent Document 2), bovine placenta extract (Patent Document 3) and the like are known as animal-derived cell activators. Examples of plant-derived cell activators include sesame seeds, sayaks, peppers, tokis, dokudami, bakumondo (patent document 4), almonds, dandelions, elderberry, senkyu, assembly, sakuhakuhi, tonin, carrots, hops, mukuge, yokuinin ( Patent Documents 5 and 6), extracts of the genus Turmeric (Patent Document 7), and the genus Hanayasuri (Patent Document 8) are known. Examples of substances having an action of promoting collagen production include licorice leaf extract (Patent Document 8), lotus germ extract (Patent Document 9), hydrolyzed potato protein (Patent Document 10), bamboo leaf extract or hawthorn. Fruit extract (Patent Document 11), rice bran solvent extract (Patent Document 12), Iridaceae Crocus saffron extract (Patent Document 13), Cucurbitaceae plant seed extract (Patent Document 14) and the like are known. . Some of these are used in quasi-drugs and cosmetics as cell activators, anti-aging agents, collagen production promoters or hyaluronic acid production promoters. However, an extracellular matrix production promoter including a cell activator, an anti-aging agent, a collagen production promoter, and a hyaluronic acid production promoter that exhibits a satisfactory effect has not been obtained.

  In addition, studies have been made to prevent or cure rough skin. Rough skin refers to dry skin in which exfoliation of keratinocytes is generally observed. Such rough skin is caused by elution of interstitial lipids such as cholesterol, ceramide, fatty acids, etc., and formation of a stratum corneum permeation barrier due to degeneration of stratum corneum caused by UV rays, detergents, etc., and disruption of proliferation / keratinization balance of epidermal cells. Etc. For the purpose of preventing or healing this rough skin, studies have been made on supplying a keratinocyte lipid component or a synthetic keratinocyte lipid similar thereto.

Polyamine is a general term for aliphatic hydrocarbons having two or more primary amino groups, and is a natural product that exists universally in the living body, and has attracted attention for its physiological action. The main physiological functions of polyamines are (1) stabilization of nucleic acids and structural changes by interaction with nucleic acids, (2) promotion of various nucleic acid synthesis systems, (3) activation of protein synthesis systems, ( 4) Stabilization of cell membranes and enhancement of membrane permeability of substances, (5) elimination of active oxygen, and (6) promotion of cell proliferation are known, but in particular, activation in skin cells (fibroblasts) The anti-aging effect, the extracellular matrix production promoting action and the collagen or hyaluronic acid production promoting action were not known at all. Moreover, it has not been reported that the whitening effect is improved by using a plant-derived polyamine in combination with a whitening agent.
JP-A-62-84024 Japanese Patent Laid-Open No. 63-188867 Japanese Patent Laid-Open No. 03-141299 Japanese Patent Laid-Open No. 10-45615 Japanese Patent Laid-Open No. 10-036279 JP 2004-75632 A JP 2005-89375 A JP 2000-191498 A JP 2002-29980 A Japanese Patent Laid-Open No. 2005-263689 JP 2006-160629 A JP 2005-272433 A JP-A-2005-206510 JP 2006-273815 A

  Giving cell activation and anti-aging effects to mammals, especially humans, is an extremely important issue, and various cell-activating substances and anti-aging agents derived from animals and plants have been found. Has not yet obtained a sufficient and stable effect to be industrially available, and a new cell activator and anti-aging agent are currently being searched.

  The object of the present invention is a plant having improved moisturizing action, collagen production promoting action, hyaluronic acid production promoting action, cell activating action, antioxidant action, anti-aging action, hair growth action, rough skin improving action, etc. The object is to provide a cosmetic composition containing the derived polyamine-containing extract. Furthermore, the objective of this invention is providing the cosmetics, quasi-drug, medical supplies, hygiene articles, and a pharmaceutical containing the said cosmetic composition.

  As a result of diligent efforts to achieve the above object, the present inventors have found that a plant-derived polyamine-containing extract, in particular, a cosmetic composition containing a polyamine-containing soybean-derived extract or wheat-derived extract is effective. It was.

  Furthermore, as a result of intensive research efforts for skin external preparations with less irritation in skin external preparations containing whitening agents, the present inventors have used whitening agents and plant-derived polyamine-containing extracts to obtain whitening. The inventors have found that the skin permeability of the preparation is improved and the effect thereof is enhanced, and that stimulation can be reduced, and the present invention has been completed.

That is, the present invention includes the following inventions (1) to (15).
(1) A cosmetic composition comprising a plant-derived polyamine-containing extract and a whitening agent.
(2) The plant-derived polyamine-containing extract is at least one material selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. The cosmetic composition according to (1), which is an extract derived from
(3) The polyamine contained in the plant extract is at least one compound selected from the group consisting of aliphatic hydrocarbons having two or more primary amino groups (1) Or the cosmetic composition as described in (2).
(4) The polyamine contained in the plant extract is 1,3-diaminopropane, putrescine, cadaverine, cardine, spermidine, homospermidine, aminopropyl cadaverine, theremin, spermine, thermospermine, canabalmin, aminopentylnorspermidine, N , N-bis (aminopropyl) cadaverine, homospermine, cardopentamine, homocardopentamine, cardohexamine and homocardohexamine (3) A cosmetic composition according to 1.
(5) The cosmetic composition according to (4), wherein the polyamine is at least one compound selected from the group consisting of putrescine, spermidine and spermine.
(6) The cosmetic composition according to any one of (1) to (5), wherein the whitening agent is a melanin production inhibitor, a melanin degradation accelerator, and / or a melanin transport inhibitor.
(7) The cosmetic composition according to any one of (1) to (6), further comprising an anti-aging agent.
(8) The cosmetic composition according to any one of (1) to (7), further comprising a moisturizing agent.
(9) The cosmetic composition according to any one of (1) to (8), further comprising an ultraviolet absorber.
(10) The cosmetic composition according to any one of (1) to (9), further comprising an antibacterial agent.
(11) The cosmetic composition according to (7), wherein the anti-aging agent is an anti-inflammatory agent and / or an antioxidant.
(12) The cosmetic composition according to (8), wherein the humectant is a hydrophilic humectant and / or a lipophilic humectant.
(13) The group in which the UV absorber is composed of a benzoic acid UV absorber, an anthranilic acid UV absorber, a salicylic acid UV absorber, a cinnamic acid UV absorber, a triazine UV absorber, and a benzophenone UV absorber. The cosmetic composition according to (9), wherein the cosmetic composition is at least one compound selected from the group consisting of:
(14) The cosmetic composition according to (10), wherein the antibacterial agent is an organic antibacterial agent and / or an inorganic antibacterial agent.
(15) The cosmetic composition according to any one of (1) to (14), which is used as a cosmetic, a quasi-drug, a medical product, a hygiene product, or a pharmaceutical product.

  The cosmetic composition containing the plant-derived polyamine-containing extract of the present invention provides moisturizing action, collagen production promoting action, hyaluronic acid production promoting action, cell activation action, antioxidant action, anti-aging action, hair growth action and rough skin improving action. It is possible to provide cosmetics, quasi-drugs, medical supplies, hygiene products, and pharmaceuticals that have effects such as these. In particular, according to the present invention, a skin external preparation with less irritation can be provided in a skin external preparation containing a whitening agent. In addition, the plant-derived polyamine-containing extract is a biologically-derived natural surfactant and thus has excellent safety, and the cosmetic composition of the present invention can sufficiently withstand long-term use. Furthermore, since a plant-derived polyamine-containing extract can be produced from a plant, the raw material cost is low, and mass production is possible.

  The cosmetic composition of the present invention contains a plant-derived polyamine-containing extract and a whitening agent as essential components.

  In the present invention, the “plant-derived polyamine-containing extract” is a plant extract containing a (natural) polyamine obtained from a plant and / or a processed plant product. In the present invention, the “plant-derived polyamine-containing extract” is sometimes referred to as “a plant extract containing a polyamine and / or a plant extract containing a polyamine as an active ingredient”. In the present invention, the “plant extract” is a product obtained by extracting a plant and / or a processed plant product. The polyamine concentration contained in the plant-derived polyamine-containing extract is usually from 0.00001 to 5M, preferably from 0.00005 to 3M, more preferably from 0.0001 to 2M in M (mol / liter). Or in the weight%, it is 0.0001 to 95% normally, Preferably it is 0.001 to 75%, More preferably, it is 0.01 to 50%.

  “Plant-derived polyamine-containing extract” can be used either as a low-purity product that has not been purified to increase the polyamine concentration, or as a high-purity product that has been purified with an ion exchange resin to significantly increase the polyamine concentration. Good. Polyamine concentration in low-purity soybean / wheat germ extract powder is about 0.1-0.2%, but polyamine concentration in high-purity purified soybean / wheat germ extract powder purified with ion exchange resin Can be increased to 30-50%. When the extract is an aqueous solution, it becomes 1/10 to 1/100 of the above concentration.

  In the present invention, “polyamine” is a general term for aliphatic hydrocarbons having two or more primary amino groups, and is a natural product that exists universally in the living body, and more than 20 types of polyamines have been found. Yes. For example, 1,3-diaminopropane, putrescine, cadaverine, cardine, spermidine, homospermidine, aminopropyl cadaverine, theremin, spermine, thermospermine, canabalmin, aminopentylnorspermidine, N, N-bis (aminopropyl) cadaverine, homo Examples include spermine, cardopentamine, homocardopentamine, cardohexamine, and homocardohexamine. Typical polyamines include putrescine, spermidine and spermine.

  “Putrescine” is one of the typical polyamines and is a general natural product that is universally present in living organisms, and is an aliphatic hydrocarbon compound having two primary amino groups. “Spermidine” is one of typical polyamines and is a general natural product that is ubiquitously present in living organisms, and is an aliphatic hydrocarbon compound having three primary amino groups. “Spermine” is one of typical polyamines and is a general natural product that is ubiquitous in living organisms, and is an aliphatic hydrocarbon compound having four primary amino groups.

  A plant extract containing a polyamine-containing plant extract and / or a plant extract containing a polyamine as an active ingredient is not particularly limited, and examples thereof include dicotyledonous plants, monocotyledonous plants, herbaceous plants, woody plants, Examples include cucurbitaceae plants, solanaceous plants, gramineous plants, cruciferous plants, legumes, mallows, chrysanthemums, rhododendrons, plant extracts, plant extracts, and the like. Specifically, sweet potato, tomato, cucumber, pumpkin, melon, watermelon, tobacco, Arabidopsis, pepper, eggplant, bean, taro, spinach, carrot, strawberry, potato, rice, corn, alfalfa, wheat, barley, soybean, rape , Sorghum, eucalyptus, poplar, kenaf, sugarcane, sugar cane, sugar beet, cassava, sago palm, red almond, lily, orchid, carnation, rose, chrysanthemum, petunia, torenia, snapdragon, cyclamen, gypsophila, geranium, sunflower, shiba, cotton, Enokitake, Honshimeji, Matsutake, Shiitake, Mushrooms, Ginseng, Agaricus, Turmeric, Ginseng, Citrus, Green Tea, Black Tea, Oolong Tea, Banana, Kiwi, Natto, Soy Milk, Soybean Extract, Wheat Echi , Germ extract, embryo extract, fruit juice, Ocala, rice germ, wheat germ, barley germ, soybean germ, corn germ, milo germ, such as sunflower germ, and the like.

  Preferable plants are monocotyledonous plants and dicotyledonous plants, more preferably grasses and legumes. Particularly preferred plants or processed products thereof include corn, mushrooms, soybean, wheat, natto, soy milk, okara, wheat germ, soybean germ, corn germ, soybean extract, wheat extract, germ extract and embryo extract. More preferably, soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat embryo, wheat germ, soy milk, and okara are mentioned.

  The plant tissue for obtaining a plant extract containing polyamine and / or a plant extract containing polyamine as an active ingredient is not particularly limited. Preferably, the seed is in the form of seed or growth process. In the case of a plant in the growth process, the plant extract of the present invention can be obtained from the whole plant or a partial tissue. The site that can be obtained is not particularly limited, and examples thereof include whole trees, flowers, buds, ovary, fruits, leaves, cotyledons, stems, buds, roots, seeds, dried seeds, embryos, germs, and the like. Preferred are fruits, leaves, stems, buds, seeds, dried seeds, embryos, embryos, and particularly preferred are seeds, dried seeds, germs, embryos, and the like.

  The plant obtained from the plant extract containing polyamine and / or the plant extract containing polyamine as an active ingredient may be a processed plant product. The processing methods are: plant impregnation using water, organic solvent, mixture of water and organic solvent, etc. at low temperature, room temperature, warming condition, distillation method, pressing method, ultrasonic method, supercritical fluid method Extract the extract by subcritical fluid method. Furthermore, the processed material etc. which fermented the extract collect | recovered from the plant and the plant are included. For example, plant extracts, soy milk, okara, wheat flour, fermented extract, natto and the like can be mentioned.

Examples of a method for obtaining a plant extract containing a polyamine and / or a plant extract containing a polyamine as an active ingredient include an extraction method by adding an acid solution to a plant and / or a processed plant product so as to be in an acidic condition. be able to. Acidic conditions refer to conditions where the pH is 6 or less. By setting the pH to acidic conditions during extraction, the polyamine composition can be efficiently and stably recovered from the plant tissue. This effect can be obtained uniformly when the pH is 6 or less, but preferably the pH is 4 or less, and particularly preferably the pH is 2 or less. The lower limit of pH corresponds to the pH of the stock solution of the acid solution to be used and is not particularly limited, but is preferably pH 0-2.
By obtaining a plant extract under acidic conditions (acid aqueous solution), the recovery rate of polyamine amount is higher than that of plant extracts collected with organic solvents such as ethanol and methanol, and it is a compound polyamine that is particularly difficult to dissolve in water. Can be solubilized in water under acidic conditions, and the content of free polyamines with low extraction efficiency is improved with organic solvents. Polyamines exhibit excellent stability under acidic conditions, and improve the stability of polyamines and other active ingredients contained in plant extracts. Furthermore, by obtaining a plant extract under acidic conditions (acid aqueous solution), natural active ingredients other than polyamines are simultaneously recovered. For example, natural active ingredients include saccharides such as monosaccharides and oligosaccharides, peptides and proteins. By containing polyamine and a natural active ingredient other than polyamine at the same time, cell activation and extracellular matrix (collagen / hyaluronic acid) production promoting effect are further enhanced. In addition, since the recovered plant extract is an aqueous solution, a high merit is expected in terms of safety as compared with an organic solvent.

  Acid solutions to be added under acidic conditions include mineral acids such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, propionic acid, butyric acid, succinic acid, malonic acid, succinic acid, fumaric acid, maleic acid, malic acid, lactic acid Organic acids such as tartaric acid, citric acid, benzoic acid and acidic water, preferably 0.01N-6N hydrochloric acid, sulfuric acid, nitric acid, acetic acid, phosphoric acid, trichloroacetic acid, sulfosalicylic acid, formic acid, citric acid, Inorganic acids and organic acids such as lactic acid and 0.1 to 10% perchloric acid, particularly preferably 0.0625 to 1N hydrochloric acid, 0.25 to 5% perchloric acid, 0.1 to 10% 1N sulfuric acid or the like.

  In the extraction step, a polyphenol adsorbent may be added. By adding a polyphenol adsorbent, it is possible to facilitate recovery of a plant extract containing polyamine and / or a plant extract containing polyamine as an active ingredient. The polyphenol adsorbent is not particularly limited as long as it is a substance that can adsorb polyphenols, but PVPP (polyvinyl polypyrrolidone), PVP (polyvinyl pyrrolidone), PEG (polyethylene glycol) and the like are preferably used. Particularly preferred is PVPP (polyvinyl polypyrrolidone). These polyphenol adsorbents may use commercially available materials. For example, Polyclar (registered trademark), POLYCLAR (registered trademark) (manufactured by ISP), Divergan (registered trademark) (manufactured by BASF), Dowex (registered trademark) -1, PVP-40, or the like may be used. By adding a polyphenol adsorbent after adding an acid solution to a plant and / or processed plant product, the recovered amount, yield, and purity of the plant extract containing polyamine and / or the plant extract containing polyamine as an active ingredient are improved. It can be expected to increase. In addition, by adding polyphenol adsorbent, mixing into plant extracts such as polyphenols that act negatively on activation of cells, skin cells, antioxidant, anti-aging, collagen production, hyaluronic acid production effect Can be suppressed. The amount of polyphenol adsorbent added is preferably 0.1 to 30% (w / v), more preferably 0.5 to 20% (w / v), still more preferably 1 to 10% (w / v). It is.

  Plant extracts and / or polyamines containing polyamines are added to plants and / or processed plant products by adding an acid solution so as to be in an acidic condition, and optionally adding a polyphenol adsorbent, followed by crushing, grinding and mixing. It is possible to increase the amount of recovered plant extract containing as an active ingredient. Particularly in the case of plant tissue, it is desirable to damage the cell wall because it has a cell wall. In the case of a processed plant product or plant extract, it does not need to be crushed or crushed so as to damage the cell wall since it does not contain the cell wall. As a method for performing crushing or crushing, for example, a mixer, a blender, a homogenizer, a mortar, an ultrasonic crusher, or the like can be used.

After sufficiently extracting the polyamine contained in the plant and / or processed plant product into the acid solution (liquid fraction), the liquid fraction is separated from the residue and precipitate by centrifugation or filtration. The recovered liquid fraction contains a large amount of polyamine, and a “plant extract containing polyamine and / or a plant extract containing polyamine as an active ingredient” is obtained. If necessary, the polyamine-containing plant extract may be desalted or purified by ion exchange, membrane fractionation, gel filtration, or electrodialysis, and at least one of these methods should be performed. Thus, a higher-purity plant extract can be obtained with respect to polyamines. In the case of the ion exchange method, the plant extract is passed through a column filled with an ion exchange resin, and the polyamine as an active ingredient is separated from impurities such as amino acids, peptides, proteins, and sugars. As the ion exchange resin to be used, the ion exchange group may be a sulfonic acid group, sulfopropyl group, phosphoric acid group, carboxylmethyl group, aminoethyl group, diethylamino group, quaternary aminoethyl group, quaternary ammonium group, etc. Either a cation exchange resin or an anion exchange resin can be used. When a cation exchange resin is used, the active component polyamine is adsorbed on the cation exchange resin. Therefore, after sufficiently separating the non-adsorbed substance, an acidic solution such as sulfuric acid or hydrochloric acid or a salt such as sodium chloride is used. The active ingredient is eluted with the solution. When an anion exchange resin is used, the polyamine which is an active ingredient is not adsorbed on the anion exchange resin, so that a non-adsorbed fraction containing the active ingredient is recovered. In the case of the membrane fractionation method, cellulose, cellulose acetate, polysulfone, polyamide, polyacrylonitrile, polytetrafluoroethylene, polyester, polypropylene, etc. A plant extract is subjected to UF using an outer filtration (UF) membrane, and a permeate containing polyamine as an active ingredient is recovered. Moreover, NF of an active ingredient solution is performed and desalted using a nanofiltration (NF) membrane having a salt rejection of 30 to 80%. In the case of the gel filtration method, the plant extract is neutralized and passed through a column packed with a gel filtration carrier to recover the polyamine as an active ingredient by molecular weight fractionation. The gel filtration carrier used is a dextran-based, acrylamide-based, agarose-based, cellulose-based, polyvinyl-based, glass-based, polystyrene-based, or the like having a fractional molecular weight in the range of 100 to 100,000. In the case of electrodialysis, electrodialysis is performed by alternately supplying a plant extract and saline between the membranes partitioned by a cation exchange membrane and an anion exchange membrane. The electrodialysis conditions include an initial current density of 0.5 to 15 A / dm ² , a voltage of 0.1 to 1.5 V / tank, and the like.

  A plant extract containing a polyamine and / or a plant extract containing a polyamine as an active ingredient may be used as it is as a cell activator, an anti-aging agent, a collagen production promoter, a hyaluronic acid production promoter, or an antioxidant. It is preferably used in cosmetics and quasi-drugs (external skin preparations, bath preparations, hair restorers, etc.), medical supplies, hygiene products or pharmaceuticals. The concentration at which polyamine is blended (M: mol / liter) is determined by the degree of absorption, the degree of action, the product form, the frequency of use, etc., and is not particularly limited, but the concentration of polyamine in the cell culture fluid in cultured cells is Usually, it is 0.00001-100 mM, Preferably it is 0.00005-75 mM, More preferably, it is 0.0001-50 mM. On the other hand, in the case of cosmetic or pharmaceutical forms such as external preparations for skin applied directly to human skin, such as liquids or creams, emulsions, lotions, lotions, ointments, etc., usually 0.01-10000 μM, preferably 0.05-1000 μM More preferably, it is 0.1-100 micromol, especially 1-10 micromol. In the case of a solid preparation such as an oral preparation, the dose per dose is usually 0.01 to 10,000 μM, preferably 0.05 to 1000 μM, more preferably 0.1 to 100 μM, and particularly 1 to 10 μM. .

Since the “plant-derived polyamine-containing extract” of the present invention is derived from a natural plant, it is safer than a chemically synthesized product. Moreover, in the case of plant origin, it suffices to prepare a polyamine-containing extract from a plant, which is excellent in convenience. Furthermore, in the case of plant origin, the polyamine-containing extract contains at least three types of polyamines, putrescine, spermidine, and spermine, and it is not necessary to individually prepare each. In the case of plant origin, cultivated or commercially available plant raw materials may be used. In particular, wheat and soybean seeds are sold at low prices among plant raw materials, and a large amount of raw materials can be secured. The production cost of goods is lower than that of chemically synthesized products. In cosmetics, natural products, especially plants, are desired. Moreover, natural components other than polyamine are contained in the plant-derived extract, and the component may further enhance the effect of the polyamine. In addition, natural components other than polyamines (for example, sugars such as monosaccharides and oligosaccharides, peptides, proteins, etc.) may act as active ingredients (cell activation / anti-aging / collagen production promotion, hyaluronic acid production promotion). Be expected. Therefore, the usefulness of the cell activator or anti-aging agent which uses the plant-derived polyamine-containing extract of the present invention as an active ingredient is very high.
In the present specification, “cell activation” refers to maintaining or enhancing cell function or activity. As a result of cell activation, it is possible to suppress a decrease in cell function or cell activity, that is, to suppress cell aging. Therefore, the “activator” has utility as an “anti-aging agent”. For example, “cell activation and anti-aging” in skin cells means that skin cell wrinkles, sagging, hardening, etc. are reduced by reducing the decrease in skin cell function associated with the accumulation of structural changes in the basement membrane due to aging and photoaging. This refers to maintaining a youthful, healthy skin condition with prevention and improvement. In addition, for example, in dermal papilla cells or hair matrix cells, it is possible to suppress hair loss by maintaining the hair cycle by reducing the functional degradation of dermal papilla cells or hair matrix cells due to aging, stress, hormone balance, etc. pointing.

  The plant-derived polyamine-containing extract of the present invention contains a natural component other than polyamine in the extract, so that the odor peculiar to polyamine is reduced compared to polyamine, and the stability is increased. There is no loss of quality even when used for external goods, medical supplies, hygiene products or pharmaceutical applications.

  What is necessary is just to add the addition amount of the plant-derived polyamine containing extract in a cosmetic composition in the range which does not impair the effect | action which a plant-derived polyamine containing extract has, Usually 0.001-50 mass% is preferable, 0.01- 30 mass% is more preferable, 0.1-10 mass% is further more preferable, and 0.5-5 mass% is especially preferable.

  The cosmetic composition of the present invention contains a whitening agent in addition to the plant-derived polyamine-containing extract. The whitening agent may be a melanin production inhibitor, a melanin degradation accelerator and / or a melanin transport inhibitor.

  Examples of whitening agents include L-ascorbic acid and derivatives thereof, hydroquinone and derivatives thereof, lucinol, edetic acid and derivatives thereof, and placenta extract, t-AMCHA, acerola extract, age extract, ellagic acid or derivatives thereof, and fire bud extract , Chamomile extract, chamomile flower extract (urea), kiwi extract, glutathione, tocotrienol, ferulic acid, raspberry ketone, lucinol, walnut extract, pyridoxine dipalmitate, sulfur, kojic acid or its derivatives, glucosamine or its derivatives, hydroxycinnamic acid Or derivatives thereof, glutathione, arnica extract, urgonum extract, Sorohakuhi extract, psycho extract, bowfish extract, mycelia mycelium culture or extract thereof, linden extract, peach leaf extract, Jitsuekisu, Kujin'ekisu, Jiyuekisu, Japanese angelica root extract, Coix extract, persimmon leaf extract, rhubarb extract, moutan bark extract, witch hazel extract, horse chestnut extract, Hypericum extract, oil-soluble licorice extract and the like.

  These whitening agents can be used alone or in combination of two or more.

  The cosmetic composition of the present invention may further contain an anti-aging agent. The anti-aging agent may be an anti-inflammatory agent and / or an antioxidant.

  Examples of anti-inflammatory agents include glycyrrhizic acid and salts thereof, glycyrrhetinic acid and salts thereof, isopropylaminocaproic acid and salts thereof, allantoin, lysozyme chloride, guaiazulene, methyl salicylate, and γ-oryzanol. Antioxidants include α-carotene. , Β-carotene, γ-carotene, lycopene, cryptoxanthine, lutein, zeaxanthin, isozeaxanthin, rhodoxanthine, capsanthin, crocetin, etc .; 1,4-diazacyclooctane, 2,5-dimethylfuran, 2-methylfuran, 2,5-diphenylfuran, 1,3-diphenylisobenzofuran, α-tocopherol, β-tocopherol, γ-tocopherol, d-tocopherol, histidine, tryptophan, methionine, alanine or its alkyl ester Tannins such as dibutylhydroxytoluene, butylhydroxyanisole, ascorbic acid, tannic acid, epicatechin, epicacatechin, epicatechin gallate, epicacatechin gallate, flavonoids such as rutin, other propyl gallates, astaxanthin, carotene, tocopherol , Ascorbic acid, tetrasodium edetate, erythorbic acid, tocopherol acetate, retinol acetate, dibitylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, methylsilanol dioletocopherol / silicic acid mixture, benzyl nicotinate, photosensitizer 401 , Aspartic acid, adenosine triphosphate 2Na, aminobutyric acid, fennel extract, Dutch mustard extract, caffeine, chlorella extract, safra Extract, ginger extract, soybean extract, tiso extract, folic acid, retinol, retinol palmitate, inositol, turmeric extract, oryzanol, carotene, carrot extract, wheat ointment extract, nematode extract, chimpi extract, touki extract, toki root extract, dodami extract, Tocopherol, tocopherol nicotinate, button extract, ergocalciferol, pyridoxine dicaprylate, batyl alcohol, glycyrrhetinyl stearate, glycyrrhizic acid, glycyrrhetinic acid, diphenhydramine hydrochloride, lysozyme, aminocaproic acid, litchi extract, yocuinine, merirot extract, button extract, Toki Extract, Toki Root Extract, Senkyu Extract, Genno Chocolate Extract, Allantoin, Arnica Extract Arnica flower extract, Ogon extract, Oren extract, Odorikosou extract, Catfish ear extract, Chamomile extract, Calamine, Kawaramugi extract, Licorice extract, Guaiazulene, Gardenia extract, Kumazasa extract, Glycyrrhizic acid 2K, Stearyl glycyrrhetic acid, Gentianana extract, Black tea extract, Comfrey Extract, Comfrey Leaf Extract, Tocopherol Acetate, Methyl Salicylate, Zinc Oxide, Shikon Extract, Murasaki Root Extract, Perilla Extract, Perilla Leaf Extract, Citrus Extract, Peonies Extract, Honeysuckle Extract, Sage Extract, Kizuta Extract, Elderflower Extract, Achillea millefolium extract, assembly extract, Sakuhakuhi extract, eucalyptus extract, pyridoxine HCl, loquat leaf extract, flyfish Scan, peach leaves, fruit extract, cornflower extract, saxifrage extract, mugwort extract, lettuce extract, Roman chamomile extract, burnet extract and Kakaroru, polyamines and the like.

  These anti-aging agents can be used individually by 1 type or in combination of 2 or more types.

  The cosmetic composition of the present invention may further contain a humectant. The moisturizing agent may be a hydrophilic moisturizing agent and / or a lipophilic moisturizing agent. Examples of hydrophilic moisturizing agents include amino acids, peptides, proteins, higher alcohols and their derivatives, lipophilic moisturizing agents, phospholipids, glycolipids. Steroids may also be used.

  Examples of humectants include sodium pyridonecarboxylate, glycol, glycerin, glucose, maltose, maltitol, sucrose, fructose, xylitol, sorbitol, maltotriose, threitol, erythritol, amylolytic sugar-reducing alcohol, sorbitol, ethylene glycol, 1 , 4-butylene glycol, diglycerin, triglycerin, tetraglycerin, 1,3-butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, 1,3-propanediol, other serine, glycine, threonine, alanine, collagen, Hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipid, Lyceroglycolipid, sphingophospholipid, glycosphingolipid, linoleic acid or esters thereof, eicosapentaenoic acid or esters thereof, pectin, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, lychee mycelium culture or extraction thereof , Wheat germ oil, avocado oil, rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol, bellows oyster extract, yakuinin extract, gallow extract, typhoid extract, diatomaceous earth extract, burdock extract, mannen wax extract, arnica extract, Wheat bran, pyrrolidone carboxylic acid, tomato extract, camellia oil, soybean phospholipid, hyaluronic acid, threonine, ammonium glycolate, methylsilanol alginate, yokuinin, toki extract, toki root extract, soybean extract, asparagus Gas extract, DNA-Na, PCA-Na, RNA-Na, ashitaba extract, aspartic acid, amachiya extract, alanine, arginine, sodium alginate, altea extract, aloe vera extract, oyster extract, barley leaf extract, oyster leaf, hydrolyzed keratin, Hydrolyzed collagen, hydrolyzed conchiolin, hydrolyzed eggshell membrane, hydrolyzed egg white, hydrolyzed silk, hydrolyzed soy protein, brown algae extract, karin extract, raspberry extract, xylitol, chitosan, cucumber extract, cucumber fruit extract, guava confection extract, Quince seed extract, glycine, glycerin, glucose, grapefruit extract, grapefruit fruit extract, clematis extract, burdock extract, rice fermentation broth, chondroitin sulfate Na, fish collagena, sun Shii extract, Shiitake extract, Giant extract, Glycerin, Cystine, Cysteine, Horsetail extract, Mallow extract, Serine, Sorbitol, Soy protein, Tomato extract, Lactic acid Na, Lactobacillus, Soybean fermented extract, Urea supine / Novara extract / Almond koji, Corn oil, Honey, hyaluronic acid Na, fukuno extract, betaine, loofah extract, maltitol, maltose, mannitol, lily extract, lactoferrin, ricin, apple extract, lotus extract, royal jelly, lanolin fatty acid polyethylene glycol 1000, lanolin fatty acid isopropyl, isopropyl myristate, myristic acid Isobutyl, hexyldecanol, myristyl lactate, lanolin fatty acid, tricaprylglyceryl, oleyl alcohol, oleic acid Chilled decyl, decyl oleate, reduced lanolin, octyl decanol, almond oil, avocado oil, olive oil, oleic acid, orange rough oil, cocoa butter, carrot extract, sesame oil, sasanqua oil, safflower oil, dihydrocholesterol, squalane, stearin Cholesteryl acid, ceramide 2, evening primrose oil, sunflower oil, sunflower oil, cefnide 3, sunflower seed oil, hybrid sunflower oil, phytosphingosine, grape seed oil, jojoba oil, jojoba seed oil, mineral oil, mink oil, macadamia nut oil, meadow Examples include foam oil, eucalyptus oil, eucalyptus leaf oil, lanolin, linoleic acid, rosehip oil, petrolatum, and polyglutamic acid.

  These humectants can be used alone or in combination of two or more.

  The cosmetic composition of the present invention may further contain an ultraviolet absorber. As UV absorbers, use benzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, triazine UV absorbers, benzophenone UV absorbers, etc. Also good.

  Examples of the ultraviolet absorber include amyl benzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate, 2-ethoxyethyl paramethoxycinnamate, Octyl paramethoxycinnamate, glyceryl mono-2-ethylhexanoate diparamethoxycinnamate, isopropyl paramethoxycinnamate, diisopropyl diisopropylcinnamate ester mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxy Benzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydride Xylbenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4′-methoxy-dibenzoylmethane, 2,4,6-trianilino-p- (carbo-2′-ethylhexyl-1′-oxy) -1,3, 5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole, 2-ethylhexyl paradimethylaminobenzoate, ethyl paraaminobenzoate, topylmethoxydipenzoylmethane, oxybenzone-1, ethyl guaiazulenesulfonate, oxidation Examples thereof include zinc, sinoxate, and homomenthyl-N-acetylanthranilate.

  These ultraviolet absorbers can be used alone or in combination of two or more.

  The cosmetic composition of the present invention may further contain an antibacterial agent. The antibacterial agent may be an organic antibacterial agent and / or an inorganic antibacterial agent.

  Antibacterial agents include alum extract, halocarban, chlorophenesine, lysozyme chloride, alkyldiaminoethylglycine hydrochloride, isopropylmethylphenol, thymol, hexachlorophene, berberine, thioxolone, salicylic acid and their derivatives, benzoic acid, sodium benzoate , Paraoxybenzoic acid ester, parachlormetacresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, carboxylic acid, sorbic acid, potassium sorbate, hexachlorophene, chlorhexidine chloride, trichlorocarbanilide, photosensitizer, bis (2-pyridylthio) -1-oxide) zinc, thianthol, hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, Bruno butoxyethanol, resorcinol, azulene, salicylic acid, zinc pyrithione, sodium mononitroguayacol, fennel extract, pepper extract, cetylpyridinium chloride, etc. benzethonium chloride and undecylenic acid derivatives.

  These antibacterial agents can be used alone or in combination of two or more.

  The form of the cosmetic composition containing the plant-derived polyamine-containing extract is not particularly limited. Therefore, it may take any state such as solid, liquid, paste, jelly, and powder. In order to form such a state, it can be solidified using, for example, a gelling agent, or can be dispersed using a liquid. Moreover, a solvent can be added to make a solution, or it can be spray-dried to form a powder.

  The cosmetic composition containing the plant-derived polyamine-containing extract of the present invention has an excellent cell activation effect, collagen production promoting effect, hyaluronic acid production promoting effect, antioxidant effect, moisturizing effect and anti-aging effect. It has been confirmed that it moderately moisturizes the skin surface and keeps it firm and shiny.

  For example, the cosmetic composition containing the plant-derived polyamine-containing extract of the present invention can be used as a cosmetic. Cosmetics include cosmetic soap, shampoo, facial cleanser, rinse, eye cream, eye shadow, cream / milky lotion, lotion, perfume, funny, cosmetic oil, hair cosmetics, hair dye, paste perfume, powder, pack, cleansing cream , Shaving cream, shaving lotion, tanning oil, sunscreen oil, tanning lotion, sunscreen lotion, tanning cream, sunscreen cream, foundation, powdered perfume, blusher, mascara, eyebrow, nail cream, beautiful nail enamel, beautiful nails Examples include enamel remover, hair wash, bath cosmetic, lipstick, lip balm, eyeliner, toothpaste, deodorant, eau de cologne, hair nourishing agent, and hair restorer. Moreover, the cosmetic composition containing the plant-derived polyamine-containing extract of the present invention can also be used as an ointment or a poultice.

  Various components can be further added to the cosmetic composition containing the plant-derived polyamine-containing extract of the present invention.

  For example, a cosmetic composition containing a plant-derived polyamine-containing extract is used by dissolving in a nonionic surfactant, a lower alcohol, a polyhydric alcohol, an oil, a higher fatty acid, a higher alcohol, a synthetic ester, silicone or the like. Also good.

  Nonionic surfactants include, for example, sorbitan fatty acid esters (for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate). Glycerol, penta-2-ethylhexyl diglycerol sorbitan, tetra-2-ethylhexyl diglycerol sorbitan, etc.); glycerin polyglycerin fatty acids (eg mono cottonseed oil fatty acid glycerin, monoerucic acid glycerin, sesquioleate glycerin, glyceryl monostearate) , Α, α′-oleic acid pyroglutamate glycerin, monostearate glycerin malate, etc.); propylene glycol fatty acid esters (for example, monos Propylene glycol tea acrylate, etc.); hardened castor oil derivative; glycerin alkyl ether and the like.

  Examples of the POE-based hydrophilic nonionic surfactant include POE-sorbitan fatty acid esters (for example, POE-sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan monooleate, POE-sorbitan tetraoleate). POE sorbite fatty acid esters (eg, POE-sorbite monolaurate, POE-sorbite monooleate, POE-sorbite pentaoleate, POE-sorbite monostearate, etc.); POE-glycerin fatty acid esters (eg, POE-glycerol monostearate, POE-glycerin monoisostearate, POE-monooleate such as POE-glycerol triisostearate, etc.); POE-fatty acid esters (for example, POE-distearate) POE-alkyl ethers (for example, POE-lauryl ether, POE-oleyl ether, POE-stearyl ether, POE-behenyl ether, POE-2-octyldodecyl ether), POE-monodiolate, ethylene glycol distearate, etc. POE-cholestanol ether, etc.); Pluronic type (for example, Pluronic, etc.); POE • POP-alkyl ethers (for example, POE • POP-cetyl ether, POE • POP-2-decyltetradecyl ether, POE • POP−) Monobutyl ether, POE / POP-hydrogenated lanolin, POE / POP-glycerin ether, etc.); tetra-POE / tetra-POP-ethylenediamine condensates (for example, Tetronic, etc.); POE-castor oil-cured castor Oil derivatives (eg POE-castor oil, POE-hardened castor oil, POE-hardened castor oil monoisostearate, POE-hardened castor oil triisostearate, POE-hardened castor oil monopyroglutamic acid monoisostearic acid diester, POE -Hardened castor oil maleic acid, etc.); POE-beeswax lanolin derivatives (eg POE-sorbite beeswax etc.); alkanolamides (eg coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide etc.); POE- Examples thereof include propylene glycol fatty acid ester; POE-alkylamine; POE-fatty acid amide; sucrose fatty acid ester; alkylethoxydimethylamine oxide; trioleyl phosphate.

  Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutyl alcohol, t-butyl alcohol and the like.

  Examples of the polyhydric alcohol include divalent alcohols (for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycol, tetramethylene glycol, 2,3-butylene glycol, Pentamethylene glycol, 2-butene-1,4-diol, hexylene glycol, octylene glycol, etc.); trivalent alcohol (eg, glycerin, trimethylolpropane, etc.); tetravalent alcohol (eg, 1, 2, 6) Pentaerythritol such as hexanetriol, etc .; pentavalent alcohol (eg, xylitol, etc.); hexavalent alcohol (eg, sorbitol, mannitol, etc.); polyhydric alcohol polymer (eg, diethylene glycol, dipropylene glycol, Tylene glycol, polypropylene glycol, tetraethylene glycol, diglycerin, polyethylene glycol, triglycerin, tetraglycerin, polyglycerin, etc.); divalent alcohol alkyl ethers (for example, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol) Monobutyl ether, ethylene glycol monophenyl ether, ethylene glycol monohexyl ether, ethylene glycol mono 2-methylhexyl ether, ethylene glycol isoamyl ether, ethylene glycol benzyl ether, ethylene glycol isopropyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, ethylene glycol Dibutyl Dihydric alcohol alkyl ethers (for example, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, diethylene glycol butyl ether, diethylene glycol methyl ethyl ether, triethylene glycol monomethyl ether, triethylene glycol) Monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monobutyl ether, propylene glycol isopropyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol Dibutyl alcohol ether ester (for example, ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glycol monobutyl ether acetate, ethylene glycol monophenyl ether acetate, ethylene glycol diazebate, ethylene glycol disuccine) , Diethylene glycol monoethyl ether acetate, diethylene glycol monobutyl ether acetate, propylene glycol monomethyl ether acetate, propylene glycol monoethyl ether acetate, propylene glycol monopropyl ether acetate, propylene glycol monophenyl ether acetate, etc.); glycerin monoalkyl ether (example) Sugar alcohol (eg, sorbitol, maltitol, maltotriose, mannitol, sucrose, erythritol, glucose, fructose, starch-degrading sugar, maltose, xylitol, starch degradation) Sugar-reduced alcohol, etc.); Glysolid; Tetrahydrofurfuryl alcohol; POE-Tetrahydrofurfuryl alcohol; POP-Butyl ether; POP / POE-Butyl ether; Tripolyoxypropylene glycerin ether; POP-glycerin ether; POP-glycerin ether phosphate POP · POE-pentane erythritol ether, polyglycerin and the like.

  Oils include avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, sasanca oil, castor oil , Linseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, kiri oil, jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil , Beef tallow, sheep fat, lard, lanolin, whale wax, beeswax, carnauba wax, molasses, candelilla wax, squalane, etc. and its hardened oil, mineral oils such as liquid paraffin, petrolatum, synthesis of glycerin tripalmitate, etc. Triglycerin is mentioned.

  Examples of higher fatty acids include lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, undecylic acid, tallic acid, eicosapentaenoic acid, docosahexaene. An acid etc. are mentioned. Examples of higher alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol, oleyl alcohol, cetostearyl alcohol, jojoba alcohol, lanolin alcohol, batyl alcohol, 2-decyltetrathececinol, cholesterol, phytosterol, and isostearyl. Alcohol etc. are mentioned. Synthetic esters include, for example, cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, decyl orenate, dimethyl octanoate, cetyl lactate, myristyl lactate, etc. Is mentioned. Examples of silicone include chain polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, cyclic polysiloxanes such as decamethylcyclopolysiloxane, and three-dimensional network structures such as silicone resins.

  The cosmetic composition containing the plant-derived polyamine-containing extract of the present invention can be used in combination with quasi-drugs, medical supplies, hygiene products, and pharmaceuticals in addition to cosmetics.

  When the cosmetic composition containing the plant-derived polyamine-containing extract of the present invention is implemented in the form of a quasi-drug, medical product, hygiene product, or pharmaceutical product, the dosage form is not limited, and ampoules, capsules, powders, granules Pills, tablets, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, mousses, bathing agents, and the like.

  As quasi-drugs, medical supplies, hygiene products, and pharmaceuticals, for example, internal and external pharmaceutical preparations, skin lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, facial cleansers and skin cleansers, Hair cosmetics such as shampoo, rinse, hair treatment, hair cream, pomade, hair spray, hair conditioner, permanent agent, hair nick, hair dye, hair growth and hair restorer, foundation, white powder, funny, lipstick, blusher, eye shadow, Makeup cosmetics such as eyeliner, mascara, eyebrows, eyelashes, cosmetics for finishing such as nail polish, perfumes, bath preparations, toothpastes, mouth fresheners, mouthwash, liquid odor / deodorant, Sanitary goods, sanitary cotton, wet tissue and the like. Moreover, the cosmetic composition containing the plant-derived polyamine-containing extract of the present invention can also be used as an ointment or a poultice.

  The cosmetic composition containing the plant-derived polyamine-containing extract of the present invention is suitably applied to humans, but should be applied to animals other than humans as long as each effect can be expected. You can also.

  The cosmetic composition containing the plant-derived polyamine-containing extract of the present invention is a component that is used in quasi-drugs, medical supplies, hygiene products, pharmaceuticals, as long as the effects of the present invention are not impaired as necessary. Additives can be used in combination. These ingredients and additives include waxes, mineral oils, fatty acids, alcohols, esters, metal soaps, gums and water-soluble polymer compounds, surfactants, vitamins, amino acids, animals or plants, herbal medicines Extracts, extracts, microbial culture metabolites, α-hydroxy acids, inorganic pigments, astringents, bactericides / disinfectants, hair preparations, fragrances, pigments / colorants, sweeteners, nutrition enhancers and the like.

  Examples of waxes include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, and the like.

  Examples of mineral oils include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax, polyethylene powder, squalene, squalane, and pristane.

  For example, as fatty acids, natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid, Examples include synthetic fatty acids such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.

  For example, as alcohols, natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol , Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene Glycol, glycerin Batyl alcohol, pentaerythritol, sorbitol, mannitol, glucose, polyhydric alcohols such as sucrose and the like.

  For example, esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, lactic acid Examples include myristyl, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

  For example, examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylate.

  For example, gum and water-soluble polymer compounds include gum arabic, benzoin gum, danmar gum, guaiac oil, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, Carboxyalkylchitin, chitosan, hydroxyalkylchitin, low molecular chitosan, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose , Sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose Scan, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

  For example, as the surfactant, anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant ( Carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, ether ester) Type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactants, protein hydrolysates) Derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants, and the like.

  For example, as vitamins, retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group, thiamine hydrochloride, thiamine sulfate (vitamin B1) ), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C, ascorbic acid and its derivatives, vitamin D In the group, ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotachysterol, in vitamin E group, tocopherol and its derivatives, ubiquinones, in vitamin K group, phytonadione (vita) Emissions K1), menaquinone (vitamin K2), menadione (vitamin K3), and the like menadiol (vitamin K4).

  For example, amino acids include valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine, and the like, and sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

  For example, as an extract or extract of an animal or plant, herbal medicine, Asenyaku (Asen Yaku), Ashitaba, Acerola, Altea, Arnica, Avocado, Achacha (Amacha), Aloe, Aloe Vera, Nettle, Ginkgo (Ginkgo leaf, Ginkgo), Fennel (cage), turmeric (red gold), usba saishin (spicy), Japanese apricot (salmon plum), Japanese white beetle, waurusushi, green rose (fruit), hikiokoshi (life-prolonging grass), Japanese oak (yellow cocoon), Koganebana (Ogon), Yamazakura ( Cherry bark), yellowfin, yellow orchid, ginseng, carrot, helicopter, licorice, Dutch pepper, orange, sagebrush (long-distance), crabs (summer hay), tsurudokudami (what neck shark), enju (槐花）, mugwort (guy leaf), scallops (莪 朮), kudu (kazune), kanoko Cormorant (Valgarine), Chamomile, Kikarasuuri (Gurone), Kawara-momogi (Cervus chinensis), Licorice (licorice), Fukidan Popo (Cavity Winter Flower, Clover Winter Leaves), Raspberry, Kiwi Fruit, Japanese Clover (Kikkyo), Chrysanthemum (Chrysanthemum Flower) ), Catalpa (fruit), Citrus fruit (fruit), Tachibana (tachibana skin), cucumber, Udo or Shishido (living, solitary), apricot, wolfberry (bone skin, coconut, coconut leaf) , Clara, bittersweet, Kumazasa, grapefruit fruit, Nikkei (Kei-Kai), Keigai (Kai-Gai), Ebisugua (Keiko), Maruba morning glory or morning glory (Ken cow), safflower (Safflower), Gobaishi (Pyper), Confrey , Copaiba, gardenia, gentian, honoki (shoku), hinata taikozu (cow knee), goshuyu (burdock), burdock, butterfly Ngomushi (Gomiko), Rice, Rice bran, Wheat, Mishima Saiko (Shibahu), Saffron, Savonso, Hawthorn (Yamazako), Salamander (Samurai), Salvia, Sancynin carrot (Ginseng ginseng), Shiitake mushroom (Shiitake), Jiou (jihuang), Shikunshi (Mr. Kimiko), Murasaki (Shine), Perilla (Shiso leaf, Shiso), Oyster (柿蒂), Peonies (glaze), Psyllium (car forerunner, car front grass), Ginger (ginger), Shobu (Japanese cypress), Japanese horned potato (Sadako), Shimotsuke, Birch, Honeysuckle (Gin silver flower, Shinobu), Western ivy, Achillea millefolium, Elderberry, Redwood, Elderberry (Bone), Zeniaoi, River Kyu ), Assembly (this medicine), mulberry (mulberry white skin, mulberry leaf), jujube (large potato), soybean, taranoki, chikutsujinjin (take bamboo carrot) ), Hanasuge (knowing mother), Waremokkou (earth), Dokudami (10 medicines), Fuyumushinatsusatake (Cycium Cordyceps), red pepper, physalis (Torone), Tachikakuso, Ryokucha (green tea), kocha (tea), clove (Clove), mandarin orange (Chen bark), camellia, camellia, capsicum (panju), crested bean curd, red snapper, daidai (orange peel), bitter mulberry (gem), corn (south fur), eucommia , 杜 中葉), tomato, Nanten (Southern fruit), garlic (large sun), barley (malt), hakusen (white birch), janohi (barley winter), parsley, batata, mint (light load), hamamelis, rose , Loquat leaves, pine pods, grapes or their leaves, loofah, bodaiju, buttons (peony skin), hops, maikai , Matsuba, Maronie, Mannenrou, Mukuroji, Melissa, Merirot, Bokeh (Kiso), Palms, Peach (Peachin, Peach), Hiogi (Garden-dried), Binroju (Mellow wax), Swordfish (Mothera), Cornflower, Yukinoshita Grass), bayberry (Yellow Plum), Yashabushi (Yakuin), pearl barley (Yokuinin), mugwort, sagebrush, lavender, apple fruit, garlic mushroom (reishi), lemon fruit, forsythia (lily), forsythia, genus , Hashiridokoro (roth root), chicken tosaka, cattle / human placenta extract, pig / cow stomach, duodenum, or intestinal extract or its degradation product, water-soluble collagen, water-soluble collagen derivative, collagen hydrolyzate, Elastin, elastin hydrolyzate, water-soluble elastin derivative, silk protein, sill Protein hydrolyzate, and the like cow blood cell protein hydrolyzate.

  Examples of microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and lactic acid fermented milk powder. .

  For example, examples of the α-hydroxy acid include glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid.

  For example, inorganic pigments include anhydrous silicic acid, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow oxide Examples include iron, bengara, black iron oxide, gunjo, chromium oxide, chromium hydroxide, carbon black, and calamine.

  For example, astringents include lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid Etc.

  Examples of the bactericidal / disinfectant include acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

  For example, selenium disulfide, alkylisoquinolinium bromide, zinc pyrithione, biphenamine, thianthol, castari tincture, ginger tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate And thioglycolic acid.

  For example, as perfumes, natural animal perfumes such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, Iran essential oil, Iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil , Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil , Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petitgren essential oil, bay essential oil, vetiva essential oil, bell Vegetable fragrances such as Mott essential oil, Peruvian balsam essential oil, Boad Rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese seed mint essential oil, Other synthetic fragrances can be mentioned.

  For example, as a dye / colorant, a red cabbage dye, a red rice dye, an akane dye, an anato dye, an squid dye, a turmeric dye, an enju dye, a krill dye, an amber dye, a caramel, a gold, a silver, a gardenia dye, a corn dye, Examples include onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment and rutin.

  Examples of sweeteners include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.

  For example, examples of the nutrient fortifier include shell calcined calcium, cyanocolabamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.

  Ingredients and additives that can be blended in the cosmetic composition containing the plant-derived polyamine-containing material of the present invention include hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antibacterial agents, Coolant, stabilizer, emulsifier, animal / plant protein and its degradation product, animal / plant polysaccharide and its degradation product, animal / plant glycoprotein and its degradation product, blood flow promoter, anti-inflammatory agent / anti Examples include allergic agents, cell activators, keratolytic agents, wound treatment agents, foaming agents, thickeners, oral preparations, deodorizing / deodorizing agents, bittering agents, seasonings, enzymes, and the like.

  The cosmetic composition containing the plant-derived polyamine-containing extract of the present invention has a moisturizing effect, collagen production promoting effect, hyaluronic acid production promoting effect, cell activation effect, antioxidant effect, anti-aging effect, hair growth effect and rough skin improving effect. It can be widely used when required. The amount to be used is determined within a range where the desired moisturizing effect, collagen production promoting effect, hyaluronic acid production promoting effect, cell activation effect, antioxidant effect, anti-aging effect, hair growth effect and rough skin improving effect are sufficiently exhibited. .

  Of course, the cosmetic composition of the present invention can be used as a cosmetic composition or a cosmetic because of the above-mentioned effects. In addition, the quasi-drug composition, the medical product composition, and the hygiene product composition are used. Products, pharmaceutical compositions, functional food compositions, quasi-drugs, medical supplies, hygiene products, pharmaceutical products, and premixed products.

The following examples further illustrate the present invention in detail but are not to be construed to limit the scope thereof.
Polyamine analysis method The polyamine content in a sample can be examined by the following method. Polyamines in plants include free polyamines, compound polyamines, and conjugated polyamines, which can be analyzed with different extraction methods (Plant Cell Physiol., 43 (2), 196-206, 2002, J. Nutr). Biochem., 4, 66-70, 1993, Biosci. Biotech. Biochem., 61 (9), 1582-1584, 1997). As a specific example, a method for analyzing plant seed free polyamine will be described in detail. About 0.1 to 1.0 g of soybean seeds were diluted with an internal standard solution (1,6-hexaneamine or 1,7-diaminoheptane, internal standard amount = 7.5 or 12 nmol) and a 5% aqueous perchloric acid solution (sample raw 5-20 mL per 1.0 g body weight) and thoroughly triturate and extract at room temperature using a Polytron mixer. The grinding solution is centrifuged at 4 ° C., 35,000 × g for 20 minutes to collect the supernatant, and this solution is used as a free polyamine solution. Add 100 to 400 μL of free polyamine solution (plant extract, purified plant extract), 200 μL of saturated sodium carbonate aqueous solution, 200 μL of dansyl chloride / acetone solution (10 mg / mL) to a microtube with a screw cap and mix gently. To do. After tightly closing the stopper of the tube, it is covered with aluminum foil and heated in a water bath at 60 ° C. for 1 hour for dansylation. After allowing the tube to cool, 200 μL of an aqueous proline solution (100 mg / mL) is added and mixed. Cover with aluminum foil and reheat in water bath for 30 minutes. After cooling, nitrogen gas is blown to remove acetone, and then 600 μL of toluene is added and mixed vigorously. After leaving the tube to separate into two phases, the upper toluene layer is fractionated into 300 μL microtubes. Nitrogen gas is blown onto the collected toluene to completely remove the toluene. 200 μL of methanol is added to the tube to dissolve the dansylated free polyamine. The amount of free polyamines of putrescine, spermidine and spermine is analyzed by an internal standard method using high performance liquid chromatography connected with a fluorescence detector (excitation wavelength: 365 nm, emission wavelength: 510 nm). As the HPLC column, μ Bondapak C18 (manufactured by Waters: 0273324, 3.9 × 300 mm, particle diameter 10 μm) is used. The polyamine content in the sample is calculated by obtaining the peak areas of each polyamine and the internal standard from the standard solution and the HPLC chart of the sample, respectively.

Example 1A: Preparation of a polyamine-containing extract derived from soybean 100 g of soybean germ (manufactured by Foryou) was diluted with a diluted internal standard solution (1,7-diaminoheptane, internal standard amount = 1200 nmol) and 500 mL of 5% aqueous perchloric acid In addition, it was left at room temperature for 1 hour. Thereafter, 16 g of polyclar VT (manufactured by ISP), which is a polyphenol adsorbent, was added, and the soybean germ was sufficiently crushed with a blender mixer and then extracted under acidic conditions by leaving it at room temperature for 30 minutes. The crushed material is centrifuged at 2 ° C. and 22,000 × g for 20 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (soybean germ extract). did. The plant extract contained 23.5 mg of putrescine as polyamine, 24.0 mg of spermidine, and 9.6 mg of spermine, and the total amount was 57.1 mg. Further, the recovered plant extract was passed through a column packed with a cation exchange resin (AG 50W-X4, 200-400 mesh, H + type, manufactured by Bio-Rad), and the polyamine was adsorbed on the resin. The column was washed by flowing 0.7N NaCl / 0.1M sodium phosphate solution (pH 8.0), water and 1N hydrochloric acid sequentially. After removing the impurities, the polyamine was eluted with 6N hydrochloric acid and neutralized with 30% sodium hydroxide to obtain a purified plant extract (purified soybean germ extract). The purified plant extract contained 20.4 mg of putrescine, 22.3 mg of spermidine and 7.7 mg of spermine as polyamines, for a total of 50.4 mg. The plant extract (soybean germ extract) and the purified plant extract (purified soybean germ extract) were desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations. .

Example 1B: Preparation of soybean-derived polyamine-containing extract 6 L of 0.1 N, 0.5 N, and 1 N hydrochloric acid solutions were added to 1 kg of soybean germ (soy germ / powder type, manufactured by For You). Further, 80 g of each of Daibagan F (BASF), which is a polyphenol adsorbent, was added, and the mixture was stirred for 2 hours at room temperature with a three-one motor and extracted under acidic conditions. The stirred product was centrifuged at 12,000 × g for 30 minutes at 4 ° C., and the liquid fraction was collected, neutralized with 30% sodium hydroxide solution, and then centrifuged at 12,000 × g for 30 minutes at 4 ° C. The liquid fraction was collected, and this liquid was used as a plant extract (soybean germ extract). The plant extract containing 0.1N hydrochloric acid contained 217.9 mg of putrescine, 163.5 mg of spermidine and 34.7 mg of spermine as polyamines, for a total of 416.1 mg. The plant extract containing 0.5N hydrochloric acid contained 226.5 mg of putrescine, 170.0 mg of spermidine and 36.5 mg of spermine as polyamines, for a total of 433.0 mg. The plant extract with 1N hydrochloric acid contained 232.4 mg of putrescine, 174.4 mg of spermidine and 39.96 mg of spermine as polyamines, for a total of 446.76 mg. The plant extract (soybean germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations.
From the above results, it can be confirmed that a plant extract containing polyamine and / or a plant extract containing polyamine as an active ingredient can be recovered even at hydrochloric acid concentrations of 0.1N, 0.5N, and 1N. However, an excellent preparation method has been found.

Example 1C: Preparation of polyamine-containing extract derived from soybean 6 L of 0.1 N, 0.5 N, and 1 N sulfuric acid solutions were added to 1 kg of soybean germ (soy germ / powder type, manufactured by For You). Further, 80 g of each of Daibagan F (BASF), which is a polyphenol adsorbent, was added, and the mixture was stirred for 2 hours at room temperature with a three-one motor and extracted under acidic conditions. Centrifugation is performed at 4 ° C. and 12,000 × g for 30 minutes, and a liquid fraction is collected, neutralized with 30% sodium hydroxide solution, and then centrifuged at 4 ° C. and 12,000 × g for 30 minutes. The liquid fraction was collected, and this liquid was used as a plant extract (soybean germ extract). The plant extract containing 0.1N sulfuric acid contained 220.8 mg of putrescine, 165.7 mg of spermidine and 35.1 mg of spermine as polyamines, for a total of 421.6 mg. The plant extract with 0.5N sulfuric acid contained 223.7 mg of putrescine, 167.9 mg of spermidine and 35.5 mg of spermine as polyamines, for a total of 427.1 mg. The plant extract with 1N sulfuric acid contained 229.4 mg of putrescine, 172.2 mg of spermidine and 36.5 mg of spermine as polyamines, for a total of 438.1 mg. The plant extract (soybean germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations.
From the above results, it can be confirmed that a plant extract containing polyamine and / or a plant extract containing polyamine as an active ingredient can be recovered even at sulfuric acid concentrations of 0.1N, 0.5N, and 1N. However, an excellent preparation method has been found.

Example 2A: Preparation of a polyamine-containing extract derived from wheat 100 g of wheat germ (cultured and roasted type, manufactured by Nisshin Pharma) diluted internal standard solution (1,7-diaminoheptane, internal standard amount = 1200 nmol), 500 mL 5% perchloric acid aqueous solution was added and allowed to stand at room temperature for 1 hour. Thereafter, 16 g of polyclar VT (manufactured by ISP), which is a polyphenol adsorbent, was added, and the soybean germ was sufficiently crushed with a blender mixer and then extracted under acidic conditions by leaving it at room temperature for 30 minutes. The crushed material is centrifuged at 2 ° C. and 22,000 × g for 20 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (wheat germ extract). did. The plant extract contained 6.4 mg of putrescine, 23.9 mg of spermidine and 14.3 mg of spermine as polyamines, for a total of 44.6 mg. Further, the recovered plant extract was passed through a column packed with a cation exchange resin (AG 50W-X4, 200-400 mesh, H + type, manufactured by Bio-Rad), and the polyamine was adsorbed on the resin. The column was washed by flowing 0.7N NaCl / 0.1M sodium phosphate solution (pH 8.0), water and 1N hydrochloric acid sequentially. After removing the impurities, the polyamine was eluted with 6N hydrochloric acid and neutralized with 30% sodium hydroxide to obtain a purified plant extract (purified wheat germ extract). The purified plant extract contained 5.7 mg of putrescine as polyamine, 21.5 mg of spermidine, and 12.9 mg of spermine, for a total of 40.1 mg. Plant extracts (wheat germ extract) and purified plant extracts (purified wheat germ extract) were desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations. .

Example 2B: Preparation of a polyamine-containing extract derived from wheat Each 1 kg of wheat germ (specially defatted wheat germ, fine powder type, 20 kg, manufactured by Nisshin Pharma Co., Ltd.) 4 L each 0.1 N, 0.5 N, 1 N Of hydrochloric acid solution was added. Further, 80 g of each of Daibagan F (BASF), which is a polyphenol adsorbent, was added, and the mixture was stirred for 2 hours at room temperature with a three-one motor and extracted under acidic conditions. Centrifugation is performed at 4 ° C. and 12,000 × g for 30 minutes, and a liquid fraction is collected, neutralized with 30% sodium hydroxide solution, and then centrifuged at 4 ° C. and 12,000 × g for 30 minutes. Then, the liquid fraction was collected, and this liquid was used as a plant extract (wheat germ extract). The plant extract containing 0.1N hydrochloric acid contained 48.4 mg of putrescine, 201.6 mg of spermidine and 46 mg of spermine as polyamines, for a total of 296 mg. The plant extract with 0.5N hydrochloric acid contained 48.4 mg of putrescine, 183.6 mg of spermidine and 50.8 mg of spermine as polyamines, for a total of 282.8 mg. The plant extract with 1N hydrochloric acid contained 51.2 mg of putrescine, 204.4 mg of spermidine and 56.6 mg of spermine as polyamines, for a total of 312.2 mg. The plant extract (wheat germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.
From the above results, it can be confirmed that a plant extract containing polyamine and / or a plant extract containing polyamine as an active ingredient can be recovered even at hydrochloric acid concentrations of 0.1N, 0.5N, and 1N. However, an excellent preparation method has been found.

Example 2C: Preparation of a polyamine-containing extract derived from wheat Each 4 kg of wheat germ (specially defatted wheat germ, fine powder type, 20 kg, manufactured by Nisshin Pharma Co., Ltd.) 4 L each 0.1 N, 0.5 N, 1 N Of sulfuric acid solution was added. Further, 80 g of each of Daibagan F (BASF), which is a polyphenol adsorbent, was added, and the mixture was stirred for 2 hours at room temperature with a three-one motor and extracted under acidic conditions. Centrifugation is performed at 4 ° C. and 12,000 × g for 30 minutes, and a liquid fraction is collected, neutralized with 30% sodium hydroxide solution, and then centrifuged at 4 ° C. and 12,000 × g for 30 minutes. Then, the liquid fraction was collected, and this liquid was used as a plant extract (wheat germ extract). The plant extract with 0.1 N sulfuric acid contained 50 mg of putrescine, 195.6 mg of spermidine and 46.4 mg of spermine as polyamines, for a total of 292 mg. The plant extract with 0.5N sulfuric acid contained 50 mg of putrescine, 201.6 mg of spermidine and 56.6 mg of spermine as polyamines, for a total of 308.2 mg. The plant extract with 1N sulfuric acid contained 52.8 mg of putrescine, 197.2 mg of spermidine and 58.4 mg of spermine as polyamines, for a total of 308.4 mg. The plant extract (wheat germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.
From the above results, it can be confirmed that a plant extract containing polyamine and / or a plant extract containing polyamine as an active ingredient can be recovered even at sulfuric acid concentrations of 0.1N, 0.5N, and 1N. However, an excellent preparation method has been found.

Example 3A: Evaluation of Cell Activating Action of Plant Extract Containing Polyamine Derived from Soybean Germ A plant extract containing polyamine derived from soybean germ and / or a plant extract containing polyamine as an active ingredient is described in Example 1A. Plant extract (soybean germ extract) and purified plant extract (purified soybean germ extract) were used. The evaluation was performed according to the following procedure. Normal human skin fibroblasts were seeded in a 48-well microplate so that the number of normal human skin fibroblasts was 2.0 × 10 ⁴ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum. After culturing at 37 ° C. in a carbon dioxide concentration of 5 vol% for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary polyamine concentration was added, and further cultured for 48 hours. Next, the medium containing 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced with a medium containing 100 μg / mL and cultured for 3 hours. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in FIG. 1 as relative values with the cell activation effect in the control (water addition) as 100. In addition, the density | concentration was shown by the polyamine density | concentration.

  As is apparent from FIG. 1, the medium supplemented with soybean germ extract and purified soybean germ extract has a high cell activation / anti-aging effect on normal human skin fibroblasts. Was recognized. In particular, in soybean germ extract, 0.67 μM, 6.7 μM, 13.4 μM (putrescine concentration 9.2 μM, spermidine concentration 3.8 μM, spermine concentration 0.4 μM), 33.5 μM polyamine concentration, purified soybean germ extract In the polyamine concentrations of 7.4 μM, 14.8 μM (putrescine concentration 8.2 μM, spermidine concentration 5.8 μM, spermine concentration 0.8 μM), and 37.0 μM, both were 20% or more compared to the control (water). Significant cell activation and anti-aging effects were observed. From the above, it has been clarified that plant extracts containing these polyamines and / or plant extracts containing polyamines as active ingredients have an excellent cell activating effect. The decrease in the rate of turnover of collagen and hyaluronic acid is reduced by reducing the deterioration of fibroblasts with age, and it exhibits an extremely excellent anti-aging effect, wrinkles on the skin caused by aging, UV exposure, etc. The sagging can be effectively improved.

Example 3B: Evaluation of Cell Activating Action of Plant Extract Containing Polyamine Derived from Wheat Germ A plant extract containing polyamine derived from wheat germ and / or a plant extract containing polyamine as an active ingredient is described in Example 2A. Plant extract (wheat germ extract) and purified plant extract (purified wheat germ extract) were used. The evaluation was performed according to the following procedure. Normal human skin fibroblasts were seeded in a 48-well microplate so that the number of normal human skin fibroblasts was 2.0 × 10 ⁴ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum. After culturing at 37 ° C. in a carbon dioxide concentration of 5 vol% for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary polyamine concentration was added, and further cultured for 48 hours. Next, the medium containing 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced with a medium containing 100 μg / mL and cultured for 3 hours. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in FIG. 2 as relative values with the cell activation effect in the control (water addition) as 100. In addition, the density | concentration was shown by the polyamine density | concentration.

  As is clear from FIG. 2, the medium supplemented with wheat germ extract and purified wheat germ extract has a high cell activation / anti-aging effect on normal human skin fibroblasts. Was recognized. In particular, in wheat germ extract, 0.33 μM, 3.3 μM (putrescine concentration 0.7 μM, spermidine concentration 2.3 μM, spermine concentration 0.3 μM), 6.6 μM, 16.5 μM polyamine concentration, purified wheat germ extract In the polyamine concentration of 4.1 μM, 8.2 μM, 20.5 μM (putrescine concentration 3.5 μM, spermidine concentration 14.5 μM, spermine concentration 2.5 μM), all were 20% or more compared to the control (water). Significant cell activation and anti-aging effects were observed. From the above, it has been clarified that plant extracts containing these polyamines and / or plant extracts containing polyamines as active ingredients have an excellent cell activating effect. The decrease in the rate of turnover of collagen and hyaluronic acid is reduced by reducing the deterioration of fibroblasts with age, and it exhibits an extremely excellent anti-aging effect, wrinkles on the skin caused by aging, UV exposure, etc. The sagging can be effectively improved.

Example 4A: Evaluation of Collagen Production Ability of Plant Extracts Containing Polyamines Derived from Soybean Germ and Polyamines Derived from Wheat Germ Containing Plant Extracts and / or Polyamines Containing Polyamines Derived from Soybean Germ as Active Components The plant extract (soybean germ extract) described in Example 1A was used as the plant extract. As a plant extract containing polyamine derived from wheat germ and / or a plant extract containing polyamine as an active ingredient, the plant extract (wheat germ extract) described in Example 2A was used. For comparison with polyamine, putrescine, spermidine and spermine were used alone. The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 48-well microplate so that the number of normal human skin fibroblasts was 1.0 × 10 ⁵ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing at 37 ° C. in a carbon dioxide concentration of 5 vol% for 24 hours, after washing twice with PBS (−), the medium is replaced with a serum-free medium to which a sample of any concentration is added, and the conditions are the same for 3 or 6 days. Incubated in From the culture supernatant, type I procollagen C-terminal peptide (PIP) produced by human fibroblasts was measured with Procollagen type I C-peptide (PIP) EIA Kit (TaKaRa). Collagen production acceleration rate was determined by measuring standard products using the above ELISA kit, creating a calibration curve from the results, and determining the amount of collagen produced when the sample was added and the amount of collagen produced when no sample was added. The collagen production amount at the time of addition (when water was added as a control) was calculated as 100% for evaluation. FIG. 3 shows the results when cultured for 3 days, and FIG. 4 shows the results when cultured for 6 days. In addition, the density | concentration was shown by the polyamine density | concentration.

  As is clear from FIGS. 3 and 4, in the medium supplemented with soybean germ extract or wheat germ extract, the collagen production activity (collagen production promoting effect) of human skin was significantly increased significantly. . Compared with control (water) and various polyamines in soybean germ extract at polyamine concentrations of 6.7 μM, 13.4 μM, 33.5 μM, and wheat germ extract at 3.3 μM, 6.6 μM, and 16.5 μM. In all cases, a remarkable collagen production promoting effect was observed. From this, it was confirmed that the plant extract containing polyamine and / or the plant extract containing polyamine as an active ingredient has an excellent extracellular matrix and collagen producing action as compared with various polyamines. By applying the plant extract containing polyamine and / or plant extract skin containing polyamine as an active ingredient, the deterioration of fibroblasts accompanying aging is reduced, collagen production is promoted and the amount of collagen is maintained. be able to. As a result, it is possible to effectively improve skin wrinkles and sagging caused by aging, exposure to ultraviolet rays, and the like.

Example 4B: Evaluation of hyaluronic acid producing ability of a plant extract containing a polyamine derived from soybean germ and a polyamine derived from wheat germ The plant extract derived from soybean germ is the plant extract described in Example 1A The product (soybean germ extract) was used. As a plant extract derived from wheat germ, the plant extract (wheat germ extract) described in Example 2A was used. For comparison with polyamine, putrescine, spermidine and spermine were used alone. The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 48-well microplate so that the number of normal human skin fibroblasts was 1.0 × 10 ⁵ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing at 37 ° C. in a carbon dioxide concentration of 5 vol% for 24 hours, after washing twice with PBS (−), the medium was replaced with a serum-free medium to which a sample having an arbitrary polyamine concentration was added, and the conditions were further changed for 3 days. Cultured. Hyaluronic acid in the culture supernatant was measured. The cells on the plate were lysed with a cell lysate containing 0.5% Triton-X100, and after recovery, the amount of protein was measured (BioRad, protein assay kit), which was used as an indicator of the number of cells. For the measurement of hyaluronic acid, hyaluronic acid produced by normal human skin fibroblasts was measured by a sandwich method using hyaluronic acid binding protein (HABP) using a hyaluronic acid plate “Chugai” (Fujirebio). FIG. 5 shows the relative value of the amount of hyaluronic acid in the sample added with polyamine and plant extract, where the amount of hyaluronic acid in the sample not added with polyamine or plant extract (control (water addition)) is taken as 100. In addition, the density | concentration was shown by the polyamine density | concentration.

  As is clear from FIG. 5, it was recognized that the hyaluronic acid production rate of human skin was significantly increased in the medium to which each polyamine, soybean germ extract or wheat germ extract was added. In 3 days of culture, soybean germ extract was controlled with various polyamine concentrations at 6.7 μM, 13.4 μM, 33.5 μM polyamine concentration, and wheat germ extract was 3.3 μM, 6.6 μM, 16.5 μM polyamine concentration. In comparison, a high hyaluronic acid production promoting action was observed in all cases. Therefore, plant extracts containing polyamines and / or plant extracts containing polyamines as active ingredients are clearly superior to polyamines in producing extracellular matrix and hyaluronic acid by containing active ingredients other than polyamines. It was confirmed to have By applying a plant extract containing polyamine and / or a plant extract containing polyamine as an active ingredient to the skin, the deterioration of fibroblasts associated with aging is reduced, and the production of hyaluronic acid is promoted to increase the amount of hyaluronic acid. Can be maintained. As a result, it is possible to effectively improve skin wrinkles and sagging caused by aging, exposure to ultraviolet rays, and the like.

Example 5: Manufacture of a soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent A cosmetic liquid having the following composition was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 5), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 6: Preparation of soybean-derived polyamine-containing extract cosmetic liquid containing whitening agent and anti-aging agent A cosmetic liquid having the composition shown below was prepared by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 6), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 7: Production of soybean-derived polyamine-containing extract cosmetic liquid containing whitening agent and moisturizing agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 7), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Moisturizer: Polyglutamic acid 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 8: Preparation of soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent and an ultraviolet absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 8), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 9: Production of soybean-derived polyamine-containing extract cosmetic liquid containing whitening agent and antibacterial agent A cosmetic liquid having the following composition was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 9), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 10: Production of soybean-derived polyamine-containing extract cosmetic liquid containing whitening agent, anti-aging agent, and moisturizer A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 10), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Moisturizer: Polyglutamic acid 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 11: Production of soybean-derived polyamine-containing extract cosmetic liquid containing whitening agent, anti-aging agent and ultraviolet absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 11), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 12: Production of soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an aging agent, and an antibacterial agent A cosmetic liquid having the following composition was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 12), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 13: Production of soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent, a moisturizing agent and an ultraviolet absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 13), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Moisturizer: Polyglutamic acid 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 14 Production of Soybean-Derived Polyamine-Containing Extract Cosmetic Liquid Containing Whitening Agent, Moisturizing Agent and Antibacterial Agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 14), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Moisturizer: Polyglutamic acid 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 15: Production of a soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an ultraviolet absorber and an antibacterial agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 15), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 16: Production of soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an anti-aging agent, a moisturizer, and an ultraviolet absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 16), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Moisturizer: Polyglutamic acid 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 17: Production of soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an anti-aging agent, a moisturizer, and an antibacterial agent A cosmetic liquid having the following composition was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 17), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Moisturizer: Polyglutamic acid 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 18: Production of soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an anti-aging agent, an ultraviolet absorber and an antibacterial agent A cosmetic liquid having the following composition was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 18), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 19: Production of soybean-derived polyamine-containing extract cosmetic liquid containing a whitening agent, a moisturizing agent, an ultraviolet absorber and an antibacterial agent A cosmetic liquid having the following composition was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 19), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Moisturizer: Polyglutamic acid 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 20 Production of Soybean-derived Polyamine-containing Extract Cosmetic Liquid Containing Whitening Agent, Anti-Aging Agent, Moisturizer, Ultraviolet Absorber, and Antibacterial Agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the soybean-derived polyamine extract, a plant extract (soybean germ extract) prepared with a 1N hydrochloric acid solution described in Example 1B was used. As a control (Comparative Example 20), a serum containing no soybean germ extract was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Soybean germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Moisturizer: Polyglutamic acid 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 21 Production of Wheat-Derived Polyamine-Containing Extract Cosmetic Liquid Containing Whitening Agent A cosmetic liquid having the following composition was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 22: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent and an anti-aging agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 23: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent and a moisturizer A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Moisturizer: Polyglutamic acid 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 24 Production of Wheat-Derived Polyamine-Containing Extract Cosmetic Liquid Containing Whitening Agent and Ultraviolet Absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 25: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent and an antibacterial agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 26: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an anti-aging agent, and a moisturizer A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Moisturizer: Polyglutamic acid 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 27: Manufacture of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an anti-aging agent, and an ultraviolet absorber A cosmetic liquid having the composition shown below was prepared by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 28: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an anti-aging agent, and an antibacterial agent A cosmetic liquid having the following composition was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 29: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, a moisturizing agent and an ultraviolet absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Moisturizer: Polyglutamic acid 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 30: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, a moisturizing agent and an antibacterial agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Moisturizer: Polyglutamic acid 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 31 Manufacture of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an ultraviolet absorber and an antibacterial agent A cosmetic liquid having the composition shown below was prepared by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 32: Production of wheat-derived polyamine-containing extract cosmetic liquid containing whitening agent, anti-aging agent, moisturizer, and UV absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Moisturizer: Polyglutamic acid 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 33: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an anti-aging agent, a moisturizing agent and an antibacterial agent A cosmetic liquid having the following composition was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Moisturizer: Polyglutamic acid 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 34 Production of Wheat-Derived Polyamine-Containing Extract Cosmetic Liquid Containing Whitening Agent, Anti-aging Agent, Ultraviolet Absorber and Antibacterial Agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 35: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, a moisturizing agent, an ultraviolet absorber and an antibacterial agent A cosmetic liquid having the following composition was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Moisturizer: Polyglutamic acid 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 36: Production of a wheat-derived polyamine-containing extract cosmetic liquid containing a whitening agent, an anti-aging agent, a moisturizer, an ultraviolet absorber and an antibacterial agent A cosmetic liquid having the composition shown below was produced by a conventional method. As the wheat-derived polyamine extract, a plant extract (wheat germ extract) prepared with a 1N hydrochloric acid solution described in Example 2B was used.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Wheat germ extract 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Whitening agent: Arbutin 4.0
Anti-aging agent: CoQ10 4.0
Moisturizer: Polyglutamic acid 4.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Evaluation of serum (control zone)
Using the essence liquids of Comparative Examples 5 to 20, five panelists evaluated the spread, stickiness, moist feeling, and hardness when using this, and determined according to the following criteria. The results are shown in Table 1.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

Evaluation of serum (soybean germ extract)
Using the essences of the compositions shown in Examples 5 to 20, five panelists evaluated the spread, stickiness, moist feeling, and hardness when using this, and determined according to the following criteria: . The results are shown in Table 2. In all cases, the whitening effect was evaluated as good.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

Evaluation of serum (wheat germ extract)
Using the essences of the compositions shown in Examples 21 to 36, five panelists evaluated the spread, stickiness, moist feeling, and hardness when using this, and determined according to the following criteria: . The results are shown in Table 3. In all cases, the whitening effect was evaluated as good.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

  The cosmetic composition containing the plant-derived polyamine-containing extract of the present invention provides moisturizing action, collagen production promoting action, hyaluronic acid production promoting action, cell activation action, antioxidant action, anti-aging action, hair growth action and rough skin improving action. Cosmetics, quasi-drugs, medical supplies, hygiene products, and pharmaceuticals that have effects such as these can be provided. In addition, according to the present invention, it is possible to provide a skin external preparation with less irritation in a skin external preparation containing a whitening agent. Therefore, it is expected to contribute greatly to the industry.

The cell activation effect | action of the plant extract prepared in the Example with respect to a normal human skin fibroblast is shown. The cell activation effect | action of the plant extract prepared in the Example with respect to a normal human skin fibroblast is shown. The collagen production promotion effect | action of the plant extract prepared in the Example with respect to a normal human skin fibroblast is shown. The collagen production promotion effect | action of the plant extract prepared in the Example with respect to a normal human skin fibroblast is shown. The hyaluronic acid production promotion effect of the plant extract prepared in the Example with respect to normal human skin fibroblasts is shown.

Claims (15)

  A cosmetic composition comprising a plant-derived polyamine-containing extract and a whitening agent.   The plant-derived polyamine-containing extract is an extract derived from at least one material selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. The cosmetic composition according to claim 1, wherein the cosmetic composition is a product.   3. The polyamine contained in the plant extract is at least one compound selected from the group consisting of aliphatic hydrocarbons having two or more primary amino groups. The cosmetic composition as described.   The polyamine contained in the plant extract is 1,3-diaminopropane, putrescine, cadaverine, cardine, spermidine, homospermidine, aminopropyl cadaverine, theremin, spermine, thermospermine, canabalmin, aminopentylnorspermidine, N, N- The compound according to claim 3, wherein the compound is at least one compound selected from the group consisting of bis (aminopropyl) cadaverine, homospermine, cardopentamine, homocardopentamine, cardohexamine and homocardohexamine. Cosmetic composition.   The cosmetic composition according to claim 4, wherein the polyamine is at least one compound selected from the group consisting of putrescine, spermidine and spermine.   The cosmetic composition according to any one of claims 1 to 5, wherein the whitening agent is a melanin production inhibitor, a melanin degradation accelerator and / or a melanin transport inhibitor.   Furthermore, an anti-aging agent is contained, The cosmetic composition in any one of Claims 1-6 characterized by the above-mentioned.   Furthermore, a moisturizing agent is contained, The cosmetic composition in any one of Claims 1-7 characterized by the above-mentioned.   Furthermore, an ultraviolet absorber is contained, The cosmetic composition in any one of Claims 1-8 characterized by the above-mentioned.   Furthermore, an antibacterial agent is contained, The cosmetic composition in any one of Claims 1-9 characterized by the above-mentioned.   The cosmetic composition according to claim 7, wherein the anti-aging agent is an anti-inflammatory agent and / or an antioxidant.   The cosmetic composition according to claim 8, wherein the humectant is a hydrophilic humectant and / or a lipophilic humectant.   The UV absorber is selected from the group consisting of benzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, triazine UV absorbers, and benzophenone UV absorbers. The cosmetic composition according to claim 9, wherein the cosmetic composition is at least one compound.   The cosmetic composition according to claim 10, wherein the antibacterial agent is an organic antibacterial agent and / or an inorganic antibacterial agent.   The cosmetic composition according to any one of claims 1 to 14, wherein the cosmetic composition is used as a cosmetic, a quasi-drug, a medical product, a hygiene product, or a pharmaceutical product.

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