JP2009125346A - Medical instrument for promoting cell regeneration and its production method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a medical instrument remarkably improving the degree of success of an operation by improving the convenience of an operator, mitigating the pain and executing a function of promoting the cell regeneration at an injured site. <P>SOLUTION: This medical instrument is so formed that a needle is connected with a handle at its one side and has a sharp tip to be inserted inside a living body at the other side, the inside of the needle and a central part of the handle are formed of space parts, and a suture is passed through in the space parts of the needle and of the handle. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a medical device for promoting cell regeneration and a method for producing the same. More specifically, the present invention is produced by coating polydioxanone monofilament with collagen and dulcitol, and has an effect of promoting cell regeneration by exhibiting an effect of promoting the joining of the suture site, hemostasis and skin elasticity. Surgical suture that excels in biocompatibility, tension and slipperiness, and does not change even when stored for a long period of time, and is highly storable. In addition, a handle is coupled to one side of the needle, and a sharp tip portion that is inserted into the living body is provided on the other side. The inside of the needle and the center of the handle are formed of a space portion. The present invention relates to a medical instrument in which a suture thread penetrates a space.

During a surgical operation, various sutures are used to suture tissues such as blood vessels, internal organs, and skin. A suture can be classified into a synthetic suture and a natural suture depending on the raw material. Depending on the presence or absence of absorption in the living body, the absorptive suture that is naturally decomposed and disappears after a certain period of time after being sutured to the wound site It can be categorized as a non-absorbable suture where the suture and the tissue at the sutured wound site are not disassembled after being sutured and the suture must be removed by re-operation. Further, sutures can be classified into monofilaments and multifilaments according to their shapes.
Absorbable sutures that are absorbed into the living body have the advantage that, after absorption, no foreign matter remains at the suture site, the normal state is restored, and no reoperation is required. For this reason, absorbable sutures have recently been employed in most surgeries. This type of suture not only requires biocompatibility and appropriate tensile strength, but also has a surface to minimize friction during tissue passage or fixation after ligation. It is required to have excellent slipperiness.
As prior patents relating to sutures, Patent Document 1 discloses a high-strength PGA / PLA copolymer suture, and Patent Document 2 discloses a suture for living tissue containing a peptide. Patent Document 3, Patent Document 4, Patent Document 5, and the like disclose multifilament absorbable sutures with improved ligation properties.
However, technological developments for producing improved types of sutures that have all of biocompatibility, tension, slipperiness, and ability to promote the joining of suture sites are being continued. As a result of repeated studies to develop the most effective suture having all the above-mentioned points, the present inventors have focused on the bond between polydioxanone and collagen.
Polydioxanone is a substance that is used as a material for monofilament-type sutures, and is non-toxic to the human body because it is decomposed and absorbed and excreted into CO2 and H2O by hydrolysis in the body. Bioabsorbable sutures made of polydioxanone alone have been used for a long time, but this suture has the disadvantage that when the yarn is thinned, the strength is lowered and the suture strength is insufficient, and the tension is inadequate. It is sufficient, and in particular, it cannot be expected to promote jointing at the suture site or improve skin elasticity.
Collagen, on the other hand, is a type of fibrous protein that forms connective tissue in the body, and is present in large amounts in skin, bone, cartilage, blood vessel wall, teeth, muscles, and the like. In particular, soluble collagen derived from natural products is dissolved and absorbed in the body. Collagen is used for preventing aging and improving wrinkles because it contributes to improving the appearance of skin tissue when injected into the body.
However, a suture thread produced by combining only polydioxanone and collagen cannot be circulated while being stored for a long period of time because collagen changes in quality at the normal temperature. In addition, when considering use as a suture that should be considered for in-vivo compatibility and safety, it is difficult to use a commercially available synthetic preservative to improve the storage property of the suture. There is.
In general, a medical needle is used for suturing an incised skin, and is also used for shaping operation such as removal of wrinkles and double scissors surgery. Medical needles used in such applications are manufactured in various shapes.
Generally, a medical needle has a sharp tip on one side and a needle ear on the other side, and a suture thread can be inserted into the needle ear for use. Most of the ear part of the needle is configured to have a sharp shape so that the practitioner can grasp it.
As a prior patent relating to a medical needle, Patent Document 6 discloses a double-edged needle for surgical operation in which ears of a plurality of needles through which a suture penetrates are provided in an intermediate portion of the needle, and Patent Document 7 discloses an abdominal cavity. A suture instrument for laparoscopic surgery is disclosed in which a thread can be easily hung on a rod when the thread is twisted while rotating the rod inside a mirror.
The medical needle should be designed in consideration of the convenience of the practitioner side to be used, and functions such as pain relief on the side of the patient to be treated, and promotion of wound site cell regeneration. However, there is a problem that it is insufficient for satisfying the above-described functions, or that its design is very complicated and difficult to put into practical use.
Korean Patent Application No. 2003-0041373 Specification Japanese Unexamined Patent Publication No. 11-9678 US Pat. No. 3,736,646 US Pat. No. 3,875,937 U.S. Pat. No. 4,027,676 Korean Patent No. 0319209 Specification Korean Patent No. 0344757 Specification

In view of the above, the present inventors coated polydioxanone with collagen and then further coated with dulcitol as an antibacterial active substance derived from Nishiki, thereby being excellent in biocompatibility and skin regeneration effect. In addition to producing sutures with increased storage stability, the convenience of the practitioner and the success of the procedure are significantly increased, and the treatment site is cured quickly after the treatment. In order to develop an improved medical device, a handle is connected to one side of the needle, and a sharp tip is inserted into the living body on the other side. The inside of the needle and the center of the handle are made up of a space. The present invention was completed by manufacturing a medical instrument in which a suture thread penetrates the space portion of the needle and the space portion of the handle.
In addition, the present inventors dissolved collagen and coated polydioxanone, and then showed adhesion promotion, hemostasis action and skin elasticity improvement action at the suture site by a method of coating dulcitol as an antibacterial substance derived from Nishiki, The present invention has been completed by finding that it is possible to produce a suture that is excellent in biocompatibility, tension and slipperiness, has high storability, and quickly cures the treatment site after treatment on the skin.

The object of the present invention is excellent in biocompatibility, tension, slipperiness, ability to promote the joining of suture sites, etc., and is excellent in cell regeneration promotion effect by hemostasis effect and skin elasticity improving effect, and can be stored for a long time. An object of the present invention is to provide a suture that does not change in quality and has high storability, has a treatment site that heals quickly after treatment on the skin, and has excellent skin regenerative power.
Another object of the present invention is to provide a method for producing the above-described suture for promoting cell regeneration.

  Another object of the present invention is to provide a medical device that significantly enhances the success of the treatment by enhancing the convenience of the practitioner and performing the pain relief and wound site cell regeneration promoting functions.

The object is to connect a handle to one side of the needle and a sharp tip portion to be inserted into the living body on the other side. The inside of the needle and the center of the handle are formed of a space portion, and the space portion of the needle and This is accomplished by developing a medical device in which a suture penetrates the space of the handle.
The present invention provides a therapeutic device for promoting cell regeneration and a method for producing the same.
In the present invention, the medical device for promoting cell regeneration includes a suture for promoting cell regeneration, and a surgical operation, or a surgical operation configured in a form convenient for shaping such as removal of a heel or double scissors surgery. Including medical equipment.
In the present invention, the suture is manufactured by coating polydioxanone monofilament with collagen and then coating with dulcitol as an antibacterial active substance derived from Nishiki, and promotes joining at the suture site, hemostasis and skin elasticity. It has an effect of promoting cell regeneration by showing an improving action, and is excellent in biocompatibility, tension and slipperiness.
In the present invention, the surgical medical instrument is provided with a handle connected to one side of the needle and a sharp tip inserted into the living body on the other side, and the center of the needle and the center of the handle are space portions. The suture thread penetrates the space portion of the needle and the space portion of the handle.
The surgical suture of the present invention is produced by coating collagen on a suture made of polydioxanone (polydioxanone monofilament) and then coating dulcitol as an antibacterial active substance derived from Nishiki.
Polydioxanone is a material that has been used as a suture by itself. Therefore, it may be coated with collagen and dulcitol using a commercially available suture made of polydioxanone alone, but polydioxanone monofilament is directly produced by melting polydioxanone and discharging (spinning) through a spinneret. You can also
As the collagen, a commercially available 100% pure collagen product can be used, and any marine collagen or mammal-derived soluble collagen may be used.
It is preferable to use dulcitol separately from a commercially available synthetic material, Nishiki.
The method for separating dulcitol from Nishiki in the present invention comprises the following steps:
Nishiki leaves, stems or roots are separated by site and extracted with ethanol, and then ethanol is completely evaporated to obtain extracts for each site;
Distilled water was added to the extract of each part and fractionated with ether. The fractionated aqueous layer was concentrated, and fractionated with ethyl acetate to obtain an organic solvent layer. The organic solvent layer was lyophilized. And obtaining the extraction powder for each part,
Dissolve the extracted powder for each part in distilled water and perform silica gel column chromatography using chloroform, chloroform: ethyl acetate (1: 4 ratio) mixed solution, ethyl acetate, and methanol as solvents. Obtaining a preparative solution;
Performing thin layer chromatography on the fractionated solution for each site to obtain a fraction exhibiting antidiabetic activity;
The step of performing Sephadex LH-20 column chromatography on the fraction obtained above and collecting the fraction showing antidiabetic activity.
The manufacturing method of the surgical suture of the present invention will be specifically described. First, collagen is dissolved in water at 50 to 60 ° C., and the collagen coating solution is prepared so that the content of collagen in the solution is 40 to 70% by weight. Manufacturing. When the collagen content is less than the above range, the amount of collagen adhering to the polydioxanone monofilament is small, which is insufficient to obtain effects such as tension, the ability to promote joining at the suture site, and skin elasticity. On the other hand, when the collagen content is larger than the above range, a large amount of collagen adheres, resulting in poor slipperiness, which is one of the required characteristics of the suture thread.
A polydioxanone monofilament prepared in advance is wound around a cylindrical bobbin or drum. After adhering these to a stirrer, they are immersed in a collagen coating solution and stirred for 20 minutes to 2 hours.
When the cylindrical bobbin or drum is a perforated cylindrical bobbin, or a rod-like drum or a perforated drum, when the polydioxanone monofilament is wound and immersed in the collagen coating liquid, the coating liquid is inside. It is possible to increase the coating effect by reaching the suture thread.
The polydioxanone monofilament treated with the collagen coating solution is dried at room temperature or, if necessary, dried using a heater.
The dulcitol derived from Nishiki is dissolved in water at 50 to 60 ° C., and the dulcitol coating solution is prepared so that the content of dulcitol in the solution is 5 to 15% by weight. When the content of dulcitol is less than the above range, the antibacterial activity is lowered and the storage property of the suture is lowered, and when the content of dulcitol is larger than the above range, the storage property is not further improved.
The polydioquinone monofilament coated with collagen prepared in advance is wound around a cylindrical bobbin or drum. After adhering them to a stirrer, they are immersed in a dulcitol coating solution and stirred for 20 minutes to 2 hours.
The polydioxanone monofilament coated with collagen treated with the dulcitol coating solution is dried at room temperature or, if necessary, dried using a heater.
By the manufacturing process as described above, it is possible to manufacture a polydioxanone suture coated with collagen and dulcitol. The sutures produced in this way have the effect of promoting cell regeneration by promoting the joining of the suture site, hemostasis and skin elasticity, and are excellent in biocompatibility, tension and slipperiness and suitable for surgical use In addition, it has the advantage that it does not change even when stored for a long period of time and is highly storable, and the treatment site is quickly cured after treatment on the skin and has excellent skin regenerating power.
On the other hand, in the surgical medical instrument according to the present invention, the handle has a semi-circular or isosceles triangular cross section. The suture is prepared by dissolving collagen in purified water to obtain a coating solution containing 40 to 70% by weight of collagen, and then immersing polydioxanone monofilament in the collagen coating solution, followed by stirring. And then drying to coat the polydioxanone monofilament with collagen, and then dissolving dulcitol in purified water to produce a coating solution containing 5 to 15% by weight of dulcitol, where the collagen coated polydioxanone monofilament The suture thread is produced by a method of stirring and drying after immersing the oxanone monofilament. The needle has an outer diameter of 0.15 to 0.18 mm and an inner diameter of 0.06 to 0.08 mm.

  The medical device according to the present invention is configured in a form that is optimal and convenient for use by a practitioner in a surgical operation such as suturing or shaping operation such as heel removal or double heel surgery. Minimizes the pain of living tissue and promotes cell regeneration, significantly improves the success of the treatment, and does not change even when stored for a long period of time. It has the effect of being cured and having excellent skin regeneration.

Hereinafter, the specific configuration and operation of the present invention will be described in more detail with reference to the following examples and experimental examples. However, the following examples are only for illustrating the present invention and do not limit the scope of rights of the present invention.

Example 1: Separation and purification of dulcitol from nishikiki Each part of nishikiki leaves, stems and roots separated and washed with water, cut into small pieces, dried in the shade until no further change in weight, and dried 1 kg each was added to 1 L of ethanol, extracted at 60 ° C. for 8 hours, filtered through gauze, and then completely evaporated using a vacuum concentrator (Rotary Vacuum Evaporator; Heidolph WB2000, Germany). A site-specific extract was obtained.
200 mL of distilled water was added to the extract of each part and fractionated with ether. The fractionated aqueous layer was concentrated and then fractionated with ethyl acetate to obtain an organic solvent layer. The organic solvent layer was freeze-dried to obtain an extracted powder for each part.
The extracted powder for each part was dissolved in 100 mL of distilled water and subjected to silica gel column chromatography. As the solvent, chloroform, a mixed solution of chloroform: ethyl acetate (ratio of 1: 4), ethyl acetate, and methanol were used in this order. Each fraction was subjected to thin layer chromatography to obtain 5 fractions. Each fraction was subjected to an oral glucose tolerance test and the fraction exhibiting the best anti-diabetic activity was further subjected to Sephadex LH-20 column chromatography to obtain 5 fractions from each. . The fraction was further subjected to an oral glucose tolerance test, and fractions exhibiting the most excellent antidiabetic activity were collected.
In the oral glucose tolerance test, first, 5 week-old ICR mice were purchased and adapted for 1 week, and then each fraction was dissolved in distilled water and orally administered at a dose of 500 mg / kg. Glucose (1.5 g / kg, Sigma) was administered, and orbital blood sampling was performed at 0 minutes simultaneously with administration, followed by orbital blood sampling at 30 minutes, 60 minutes, and 90 minutes to measure blood glucose and This was done by observing. Blood was collected from the orbital vein of experimental animals using heparinized capillaries. The collected blood was centrifuged at 5000 rpm and 4 ° C. for 5 minutes and used for the experiment. Blood glucose was measured by the glucose oxidase method. The experimental animals were bred in an animal breeding ground maintained at a humidity of 50% and a temperature of 24-26 ° C. so that water was freely available.
As a result of examining the physicochemical and spectroscopic characteristics of the fraction obtained above, it was confirmed to be dulcitol.

Examples 2 to 7: Manufacture of sutures with different collagen contents Collagen derived from cattle was prepared by dissolving bovine-derived collagen in purified water at 50 ° C at 30, 40, 50, 60, 70, and 80% by weight, respectively. did. A bobbin wrapped with polydioxanone monofilament was immersed in the collagen coating solution for 30 minutes and stirred, and then dried at 22 ° C. to obtain a polydioxanone suture thread coated with collagen.
The dulcitol coating solution was prepared by dissolving 10 wt% of dulcitol in Example 1 in purified water at 50 ° C. A bobbin wrapped with collagen-coated polydioxanone suture is immersed in the dulcitol coating solution for 30 minutes and stirred, and then dried at 22 ° C. to obtain a polydioxanone suture coated with collagen and dulcitol. It was.

Examples 8 to 12: Production of sutures having different dulcitol contents Collagen derived from cattle was dissolved in purified water at 50 ° C at 50% by weight to produce a collagen coating solution. A bobbin wrapped with polydioxanone monofilament was immersed in the collagen coating solution for 30 minutes and stirred, and then dried at 22 ° C. to obtain a polydioxanone suture thread coated with collagen.
The dulcitol coating solution was prepared by dissolving the dulcitol of Example 1 in purified water at 50 ° C. at 3, 5, 10, 15, and 20% by weight, respectively. A bobbin wrapped with collagen-coated polydioxanone suture is immersed in the dulcitol coating solution for 30 minutes and stirred, and then dried at 22 ° C. to obtain a polydioxanone suture coated with collagen and dulcitol. It was.

Comparative Example 1: Production of dulcitol-untreated suture A collagen coating solution was produced by dissolving bovine collagen in purified water at 50 ° C. at 50% by weight. A bobbin wrapped with polydioxanone monofilament was immersed in the collagen coating solution for 30 minutes and stirred, and then dried at 22 ° C. to obtain a polydioxanone suture thread coated with collagen.

Experimental Example 1: Measurement of the slipperiness and friction coefficient of sutures The ligation slipperiness and the friction coefficient were measured for the sutures produced in Examples 2 to 7, respectively.
The slidability of the ligature was slid after being tied around the cylindrical rod by the (1 × 1) method in the suture ligation method proposed by Tera during the surgical operation. This is a very simple and convenient method for evaluating the slipperiness after ligation. In order to examine the ligation and slipping property in a wet state, the suture was immersed in a 25 ° C. water bath containing distilled water for 1 minute and then evaluated.
The friction coefficient was measured quantitatively by using F-meter (Model: R-1182, Rothschild (Switzerland)) as a friction coefficient measuring instrument.
Each result is shown in Table 1 below. Considering the slipperiness, it can be seen that it is preferable to produce a suture using a collagen coating solution containing 70% by weight or less of collagen.

(Table 1) Slippage and friction coefficient of sutures

Ligand slip Friction coefficient Example 2 ++ 0.17
Example 3 ++ 0.18
Example 4 ++ 0.22
Example 5 ++ 0.25
Example 6 + 0.27
Example 7-0.35

Note) ++: very good, +: good,-: normal,-: bad

Experimental Example 2: Measurement of tension after treatment with suture thread The dorsal part of a 10-week-old male rat was depilated, and a 2 cm incision was made with a scalpel in a direction perpendicular to the midline of the dorsal part. Using the sutures of Examples 2-7, the incision was sutured at five locations at the same interval. Two weeks later, the rats were sacrificed, and skin sections were collected from the center of the incision with a width of 1 cm to the left and right, and the tension was measured using a rheometer. Measurement was performed using 5 rats per suture, and the average value is shown in Table 2 below. It can be confirmed that the tension increases as the collagen content increases. Therefore, it can be seen that the suture of the present invention is excellent in the function of promoting the joining of the sutured parts.

(Table 2) Suture tension (g / cm)

Example 2 Example 3 Example 4 Example 5 Example 6 Example 7
Tension (g / cm) 191 214 225 232 251 258

Experimental Example 3: Measurement of skin elasticity and wrinkle improvement effect after suture treatment The elasticity of the dorsal site of 10-week-old male rats was measured using a cutometer (SEM474, Curage + Khazaka Electronic GmbH, Germany). Thereafter, the back part of the rat was depilated, and a 2 cm incision was made with a scalpel in a direction perpendicular to the midline of the back part, and then the incision part was cut at the same interval with the sutures of Examples 2-7. The area was sutured. Two weeks later, the elasticity of the rat's dorsal region was measured again using a cutometer. Measurement was performed using 5 rats per suture, and the elasticity improvement results before and after the suture treatment were compared, and the degree of improvement in elasticity is shown in Table 3 below in percent (%). From Table 3 below, it can be seen that the suture of the present invention is excellent in the skin elasticity improving function, and it is preferable to produce a suture using a collagen coating solution containing 40% by weight or more of collagen.

(Table 3) Improvement of skin elasticity by suture (%)
Example 2 Example 3 Example 4 Example 5 Example 6 Example 7
Elasticity improvement (%) 4 12 17 21 23 24

Experimental Example 4: Measurement of hemostasis effect after suture treatment After human blood was added to a test tube and treated with heparin, the sutures of Examples 2 to 7 were respectively added, and the coagulation phenomenon was confirmed with the naked eye. . Test tubes without sutures were compared as a control group. As a result, there was no change in the test tube without the suture thread, but clotting was observed in the test tube with the suture thread.

Experimental Example 5: Investigation of storage stability of sutures according to the present invention Storage characteristics of the sutures of Examples 8 to 12 and Comparative Example 1 were examined. Each suture was stored at 25 ° C., and it was examined whether or not it deteriorated with time, and the longest storage period was examined. The results are shown in Table 4 below.

(Table 4) Sustainable period of suture (unit: weeks)

Example 8 Example 9 Example 10 Example 11 Example 12 Comparative Example 1
Storage period 12 weeks 20 weeks 48 weeks 55 weeks 56 weeks 12 weeks

Experimental Example 6: Investigation of the duration of skin regeneration after suturing treatment according to the present invention After removing the back part of a 10-week-old male rat and making a 2 cm incision with a scalpel in a direction perpendicular to the midline of the back part Using the sutures of Examples 8 to 12 and Comparative Example 1, 5 incisions were sutured at the same interval. The degree of skin regeneration at the treatment site on the back of the rat over time, that is, the extent to which the treatment site was cured was observed. Measurement was made using 5 rats per suture, and the time required for the treatment site after the suture treatment to completely heal was investigated and shown in Table 5 below. As a result of the experiment, it can be seen that when the suture of the present invention further coated with dulcitol is treated, the healing speed of the treatment site is remarkably increased as compared with the suture not coated with dulcitol.

(Table 5) Required skin regeneration period (unit: days)

Example 8 Example 9 Example 10 Example 11 Example 12 Comparative Example 1
Duration of skin regeneration 9 days 5 days 3 days 3 days 4 days 10 days

Next, the surgical medical instrument of the present invention will be described in more detail with reference to the accompanying drawings.
FIG. 1 is a perspective view generally showing a medical device according to the present invention, and FIG. 2 is a partially enlarged view showing the medical device according to the present invention by region.
The medical instrument of the present invention comprises a handle 1, a needle 2 coupled to the handle, inserted into the living body, and a suture thread 3 penetrating through a space portion in the needle and a space portion at the center of the handle. Is done.
The distal end portion of the needle 2 is constituted by a sharp insertion portion 4 processed into a tapered shape, and the inside of the needle 2 is constituted by a space portion 5 so that the suture thread 3 can penetrate.
The handle 1 has a semicircular or isosceles triangular cross section, and has a space 6 connected to the space 5 of the needle at the center thereof, and the suture thread 3 passes therethrough.
1 shows a case where the cross section of the handle 1 is semicircular, and FIG. 2 shows a case where the cross section of the handle is an isosceles triangle.
FIG. 3 is a perspective view showing a form in which a medical device according to the present invention is covered with a packaging lid. As shown in FIG. 3, the medical device according to the present invention is preferably provided in a form in which the needle is covered with a lid made of a plastic tube in order to safely store the sharp insertion portion of the needle.
FIG. 4 is a state diagram showing a form in which a practitioner uses the medical instrument according to the present invention.
A method for introducing a suture into the body using the medical instrument of the present invention is as follows. As shown in FIG. 4, after grasping the handle 1 and bending the distal end portion of the suture thread 2, the needle insertion portion 4 is pierced and penetrated through the target site in the living body to be treated, and the suture thread 3 is moved together with the needle 2. After being put into the living body, the needle is returned to the original position and removed, and the remaining suture is cut with scissors. By such a method, the suture thread is introduced into the target site, and the effect of promoting the joining and cell regeneration of the suture site is exhibited.
The handle 1 is made of rubber, plastic or metal, and preferably has a semicircular diameter or a bottom diameter of an isosceles triangle of 2.5 to 3.5 cm.
Since the handle has a semicircular or isosceles cross-sectional shape and has a diameter in the above-described range, as shown in FIG. 4, the practitioner grasps the handle with one hand and positions the instrument at the target site. It is easy to adjust accurately, and it is convenient without hand fatigue during the treatment.
The handle can have various shapes as shown in FIGS.
That is, as shown in FIGS. 5 to 12, the handle of the medical device of the present invention has a perfect circle (FIG. 5), a right triangle (FIG. 6), a right angle (FIG. 7), a rectangle (FIG. 8), and an equilateral triangle. (FIG. 9), a star shape (FIG. 10), a parallelogram (FIG. 11) or a hexagonal shape (FIG. 12) is formed with a certain thickness, and a through hole is provided on one side of the outer peripheral surface. A needle is inserted therein, and the suture thread is configured to be used through the handle and the needle space.
Moreover, as shown in FIG. 13, the handle of the medical device of the present invention includes a through pipe between two rectangular plates forming a certain thickness, and a through hole is provided on one side together with the through pipe. A needle is inserted therein, and the suture thread is configured to be used through the handle space and the needle space.
As shown in FIGS. 14 and 15, the handle of the medical device of the present invention is composed of a pair of two discs having a certain thickness, and a through hole is provided on one side thereof. A needle is inserted into the handle, and the suture thread is configured to be used through the space between the handle and the needle.
The needle 2 is made of a metal material, and preferably has an outer diameter of 0.15 to 0.18 mm and an inner diameter of 0.06 to 0.08 mm.
Since the outer diameter and inner diameter of the needle are fine as described above, pain can be minimized before and after the treatment. If the outer diameter and inner diameter of the needle are larger than the above range, it is unsuitable for operation without anesthesia because of pain, and if the outer diameter and inner diameter of the needle are smaller than the above range, the needle is not attached to living tissue. It is not easy to stab and penetrate.
The suture thread 3 is obtained by dissolving collagen in purified water to obtain a coating solution containing 40 to 70% by weight of collagen, then immersing polydioxanone monofilament in the collagen coating solution, and then stirring and drying. And coating polydioxanone monofilament with collagen, and then dissolving dulcitol in purified water to produce a coating solution containing 5 to 15% by weight of dulcitol, where the polydioxanone coated with the collagen is prepared. It is a suture manufactured by a method of stirring and drying after dipping a monofilament.
The sutures manufactured in this way are excellent in biocompatibility, tension and slipperiness, and can be used effectively in surgery, and promote cell regeneration by promoting the joining of the suture site, hemostasis and skin elasticity. In addition, it has the advantage of being excellent in storability and skin regeneration.
The medical device of the present invention configured as described above has the effect of enhancing the convenience of the practitioner and significantly improving the success of the treatment by performing pain relief and a wound site cell regeneration promoting function.

  As described above, the medical device manufactured by the method of the present invention is an epoch-making medical device that promotes cell regeneration. In particular, a suture is superior in biocompatibility, tension, and slipperiness, so that surgery is performed. It can be used effectively for the purpose of promoting the regeneration of cells by promoting the joining of the suture site, hemostasis and skin elasticity, and has an advantageous effect on the health and beauty of the human body. Constructed in a form that is optimal and convenient for use by the practitioner in suturing or shaping procedures such as heel removal, double scissors surgery, etc., and minimizes pain in the living tissue being treated and cell regeneration It has the effect of remarkably enhancing the success of the treatment by promoting the treatment, and it has high storability without deterioration even when stored for a long period of time. effect When viewed from that, this is a very useful invention on the cosmetic and medical industries.

It is a perspective view which shows the case where the cross section of the handle | steering_wheel of the medical device which concerns on this invention is semicircle shape. It is the perspective view which shows the case where the cross section of the handle | steering_wheel of the medical device which concerns on this invention is an isosceles triangle shape, and the enlarged view which showed this partially. It is a perspective view which shows the form which covered the packaging lid | cover on the medical device which concerns on this invention. It is a state figure which shows the form which a practitioner uses the medical instrument which concerns on this invention. When the handle | steering-wheel of the medical device which concerns on this invention forms fixed thickness in perfect circle shape, it is a state figure which shows the form which a practitioner uses the medical device which concerns on this invention. When the handle | steering-wheel of the medical device which concerns on this invention forms fixed thickness in a right triangle shape, it is a state figure which shows the form which a practitioner uses the medical device which concerns on this invention. When a handle of a medical instrument according to the present invention forms a certain thickness in a right angle shape, it is a state diagram showing a form in which a practitioner uses the medical instrument according to the present invention. When the handle | steering-wheel of the medical device which concerns on this invention forms fixed thickness in a rectangular shape, it is a state figure which shows the form which a practitioner uses the medical device which concerns on this invention. When the handle | steering-wheel of the medical device which concerns on this invention forms fixed thickness in equilateral triangle shape, it is a state figure which shows the form which a practitioner uses the medical device which concerns on this invention. When the handle | steering-wheel of the medical device which concerns on this invention forms fixed thickness in star shape, it is a state figure which shows the form which a practitioner uses the medical device which concerns on this invention. When the handle | steering-wheel of the medical device which concerns on this invention forms fixed thickness in parallelogram shape, it is a state figure which shows the form which a practitioner uses the medical device which concerns on this invention. When the handle | steering-wheel of the medical device which concerns on this invention forms fixed thickness in hexagonal shape, it is a state figure which shows the form which a practitioner uses the medical device which concerns on this invention. FIG. 6 is a state diagram showing a form in which a practitioner uses a medical instrument according to the present invention when a penetrating tube is formed between two rectangular plates in which the handle of the medical instrument according to the present invention forms a certain thickness. It is a perspective view which shows the case where the handle | steering_wheel of the medical device which concerns on this invention consists of a pair of two discs, and the through-hole is provided in one side. When two discs in which the handle of the medical instrument according to the present invention forms a certain thickness are paired and a through hole is provided on one side, the practitioner uses the medical instrument according to the present invention. It is a state diagram which shows a form.

Explanation of symbols

1: Handle 2: Needle 3: Suture 4: Insert part 5: Space part 6: Space part

Claims (10)

Dissolving collagen in purified water to obtain a coating solution containing 40 to 70% by weight of collagen;
After immersing polydioxanone monofilament (polydioxanone monofilament) in the collagen coating solution, stirring and drying, producing a collagen-coated polydioxanone monofilament,
Dissolving the dulcitol in purified water to produce a coating solution containing 5-15 wt% dulcitol;
And a step of immersing the collagen-coated polydioxanone monofilament in the dulcitol coating solution, followed by stirring and drying.   A suture for promoting cell regeneration produced by the method according to claim 1. A handle is connected to one side of the needle, and the other side has a sharp tip inserted into the living body.
The inside of the needle and the center of the handle is a space,
A medical instrument, wherein a suture thread penetrates the space portion of the needle and the space portion of the handle. The medical device according to claim 3, wherein the handle has a semicircular or isosceles triangular cross section. The handle has a certain thickness in the shape of a perfect circle, right triangle, right angle, rectangle, equilateral triangle, star shape, parallelogram or hexagon, and a through hole is provided on one side of the outer peripheral surface. 4. The medical instrument according to claim 3, wherein a needle is inserted therein and the suture thread is configured to be used through the handle and the needle space. The handle includes a through tube between two rectangular plates having a constant thickness, a through hole is provided on one side together with the tube, a needle is inserted therein, and a suture thread is attached to the handle and the needle. The medical device according to claim 3, wherein the medical device is configured to be used through the space portion. The handle has a pair of two disks having a certain thickness, a through hole is provided on one side, a needle is inserted into the inside, and a suture thread passes through the space between the handle and the needle. The medical device according to claim 3, wherein the medical device is configured to be used through. The medical instrument according to claim 3, wherein the suture is the suture for promoting cell regeneration according to claim 2. The medical device according to claim 3, wherein the needle has an outer diameter of 0.15 to 0.18 mm and an inner diameter of 0.06 to 0.08 mm. The method of using the medical device according to claim 3, wherein the distal end portion of the suture is bent, the distal end portion of the needle is pierced through the subcutaneous tissue, the needle is removed, and the remaining suture is cut with scissors.