JP2009107946A - Lutein degradation inhibitor and food and drink containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a highly safe degradation inhibitor of lutein, having excellent activities for preventing the degradation of the lutein (such as activities for preventing photodegradation of the lutein), and to provide a food and drink utilizing the degradation inhibitor of the lutein. <P>SOLUTION: The degradation inhibitor of the lutein contains an extract of Astragalus complanatus R.Br. The food and drink contain the degradation inhibitor of the lutein. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a lutein degradation inhibitor containing an extract of vine and a food or drink containing the lutein degradation inhibitor.

Lutein is a macular pigment present in the retina and macular region, and is known to be effective in treating or preventing eye function enhancement, cataracts, age-related macular degeneration, and the like (see, for example, Patent Documents 1 and 2). . Since lutein cannot be produced in the body, it is necessary to take it with meals.For this reason, lutein is included in many supplements as an eye care material, but it is not available for active oxygen, stress, aging, strong light, etc. There was a problem that it was easy to decrease due to a factor.
Therefore, the present situation is that development of a lutein degradation inhibitor capable of preventing the degradation of lutein due to the external factors as described above and maintaining the effect of lutein is desired.

JP 2005-287376 A JP 2003-238442 A

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention provides an excellent lutein deterioration preventing action (such as lutein light deterioration preventing action) and a highly safe lutein deterioration preventing agent, and a food and drink using the lutein deterioration preventing agent. The purpose is to do.

As a result of intensive studies to solve the above problems, the present inventors have found that an extract of Astragalus complanatum R. Br. Has an excellent anti-degradation effect on lutein (such as an anti-deterioration effect of lutein on light). As a result, the present invention has been completed.
Astragalus complanatas R. Br. Is a plant belonging to the genus Leguminous genus, and is also called a perennial, and is a perennial that grows in mountains such as China. The plant height is 1m or more, the whole is covered with short bristles, and the stem is slightly flat and grows on the ground. The seeds of turrengue have a rounded kidney shape, which is also called shaenshi, and is said to have medicinal properties such as supplementing liver and kidney functions. However, it has not been known at all that an extract of turrengue has an excellent action to prevent deterioration of lutein, which is a new finding by the present inventors.

The present invention is based on the above findings of the present inventors, and means for solving the above problems are as follows. That is,
Lutein deterioration preventing agent characterized by containing an extract of <1> Tsururenge (Astragalus complanatus R.Br.).
<2> The lutein degradation inhibitor according to <1>, wherein the lutein has a light degradation prevention effect.
<3> A food or drink comprising the lutein degradation inhibitor according to any one of <1> to <2>.
<4> Furthermore, it is the food / beverage products as described in said <3> containing a lutein.

  According to the present invention, various problems in the prior art can be solved, the above-mentioned object can be achieved, lutein degradation preventing action (such as lutein light degradation preventing action) and the like, and high safety lutein degradation prevention. A food and drink using the agent and the lutein degradation inhibitor can be provided.

(Lutein degradation inhibitor)
The lutein deterioration preventing agent of the present invention contains an extract of turrengue and further contains other components as necessary.

  The lutein deterioration preventing agent has at least the effect of preventing the light deterioration of lutein. The details of the substance that exhibits the anti-light degradation effect of lutein, which is considered to be present in the extract of turrengue, is unknown. However, the extract of turrengue has such an excellent action, and it deteriorates lutein. Useful as an inhibitor has never been known before, and is a new finding by the present inventors.

The Astragalus complanatas R. Br. Is a plant belonging to the genus Leguminous genus and is also called a perennial, and is a perennial plant that grows in mountains such as China. The plant height is 1m or more, the whole is covered with short bristles, and the stem is slightly flat and grows on the ground. The seeds of turrengue have a rounded kidney shape, which is also called shaenshi, and is said to have medicinal properties such as supplementing liver and kidney functions.
The vines grow in mountain areas such as Liaoning, Jilin, Hebei, Shaanxi, Gansu, Shanxi, Inner Mongolia, etc. in China and are easily available from these areas.

  There is no restriction | limiting in particular as the site | part of the said vine genus used as an extraction raw material, According to the objective, it can select suitably, For example, using a leaf part, a flower part, a stem part, a fruit, a seed, a root part, etc. it can. Among these, seeds are particularly preferable.

  For example, the vine root, which is an extraction raw material, can be subjected to solvent extraction in a state as it is or after pulverization using a crusher after drying. The drying may be performed, for example, in the sun or using a commonly used dryer. In addition, you may use the said vine roots as an extraction raw material, after performing pretreatments, such as degreasing, with nonpolar solvents, such as hexane and benzene. By performing a pretreatment such as degreasing, the extraction process with a polar solvent of vine can be efficiently performed.

  The extract of vines can be easily obtained by using a method generally used for plant extraction. It should be noted that the extract of the vine genus includes an extract of violet genus, a diluted or concentrated solution of the extract, a dried product of the extract, or a crude product or a purified product thereof.

  There is no restriction | limiting in particular as a solvent used for extraction, According to the objective, it can select suitably, For example, water, a hydrophilic organic solvent, or these mixed solvents etc. can be used.

  The water that can be used as the extraction solvent is not particularly limited and can be appropriately selected depending on the purpose. For example, pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, etc. In addition, those subjected to various treatments are included. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. The water that can be used as the extraction solvent includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  The hydrophilic organic solvent that can be used as the extraction solvent is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include lower ones having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol. Alcohols: lower aliphatic ketones such as acetone and methyl ethyl ketone; polyhydric alcohols having 2 to 5 carbon atoms such as 1,3-butylene glycol, propylene glycol, glycerin, etc., and mixed solvents of these hydrophilic organic solvents and water Etc. can also be used. In addition, when using the mixed solvent of the said water and the said hydrophilic organic solvent, in the case of a lower alcohol, 1 mass part-90 mass parts with respect to 10 mass parts of water, and the water 10 in the case of a lower aliphatic ketone. It is preferable to use what mixed 1 mass part-40 mass parts with respect to the mass part. Moreover, in the case of polyhydric alcohol, it is preferable to use what mixed 1 mass part-90 mass parts with respect to 10 mass parts of water.

When extracting an extract having at least the action of preventing photodegradation of lutein from the vine root, which is an extraction raw material, it is not necessary to adopt a special extraction method, and it is extracted using an arbitrary extraction device at room temperature or under reflux heating. can do.
Specifically, in the treatment tank filled with the extraction solvent, the vine root as an extraction raw material is added, and further, with occasional stirring as necessary, after standing for 30 minutes to 2 hours to elute soluble components, A solid substance is removed by filtration, and the extract can be obtained by evaporating the extraction solvent from the resulting extract and drying. The amount of the extraction solvent is usually 5 to 15 times (mass ratio) of the extraction raw material. The extraction conditions are usually about 1 to 4 hours at 50 to 95 ° C. when water is used as the extraction solvent. Moreover, when the mixed solvent of water and ethanol is used as an extraction solvent, it is normally 30 minutes-about 4 hours at 40 to 80 degreeC. The extract obtained by extraction with a solvent can be used as it is as the lutein degradation inhibitor of the present invention as long as the extraction solvent is highly safe.

  In order to obtain a dilute or concentrated solution of the extract, a dried product of the extract, or a crudely purified or purified product of these extracts, the vine extract obtained by extraction is diluted, concentrated, dried and purified according to a conventional method. Etc. may be performed. In addition, although the extract of the obtained vine rengue can be used as it is as a lutein deterioration preventing agent as it is, a concentrated solution or a dried product thereof is easier to use. In obtaining the dried extract, a conventional method can be used, and carriers such as dextrin and cyclodextrin may be added to improve hygroscopicity. In addition, it is possible to perform purification for the purpose of decolorization, deodorization, etc. within a range that does not cause a decrease in the physiological activity of the extract of vines, but there is no practical problem even if it is not purified. Specifically, purification can be performed by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like.

The extract of vines obtained as described above has at least the effect of preventing the photodegradation of lutein, and based on this action, it can be suitably used as an active ingredient of the lutein degradation inhibitor of the present invention.
In addition, the content of the extract of turrengue in the lutein degradation inhibitor is not particularly limited and can be appropriately selected according to the purpose. The lutein degradation inhibitor is an extract of turrengue. The thing itself may be sufficient.

  In addition, other components other than the extract of the vine that can be contained in the lutein degradation inhibitor are not particularly limited as long as they do not impair the effects of the present invention, and are appropriately selected depending on the purpose. For example, a physiological saline solution for diluting the above-mentioned extract of the crane rengue to a desired concentration can be used. Moreover, there is no restriction | limiting in particular also in content of the said other component in the said lutein degradation inhibitor, According to the objective, it can select suitably.

  Since the lutein deterioration preventing agent of the present invention has an excellent lutein deterioration preventing action and is excellent in safety, it is suitable for use in, for example, the food and drink of the present invention described later.

(Food)
The food / beverage product of this invention contains the above-mentioned lutein degradation inhibitor of this invention, Preferably it contains further lutein, and also contains another component suitably as needed.
Here, the food and drink are those which are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to the category of products, etc., and means, for example, a wide range of foods that are taken orally, including general foods, health foods, health functional foods, quasi drugs, pharmaceuticals, and the like.
The food or drink may be obtained by blending a vine extract with any food or drink so as not to impede its activity, or may be a dietary supplement mainly composed of a vine extract. . Moreover, the said food / beverage products may be an extract of a vine.

  There is no restriction | limiting in particular as said food-drinks, According to the objective, it can select suitably, For example, drinks, such as a soft drink, a carbonated drink, a nutritive drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet, shaved ice, etc. Noodles such as buckwheat, udon, harusame, gyoza skin, sushi mai, Chinese noodles, instant noodles; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, Sweets such as bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red sea bream, scallops, abalone, sea urchin, sea urchin, salmon roe, etc .; Processed fishery and livestock products such as dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, and short nibs Fats and processed oils such as whipped cream and dressings; seasonings such as sauces and sauces; curry, stew, oyakodon, rice bowl, miso cooked, Chinese rice bowl, and rice cake, tempura, eel rice, hayashi rice, oden, marvodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger, meatballs; various forms of health foods and nutritional supplements; pharmaceuticals such as tablets, capsules, drinks, troches, etc. Examples include foreign products.

Moreover, since the said lutein degradation inhibitor is excellent in the lutein degradation prevention effect | action, it is preferable to be applied to the food / beverage products containing lutein especially. Lutein is known to be effective in enhancing eye function, treating or preventing cataracts, age-related macular degeneration, etc., but it deteriorates due to external factors such as active oxygen, stress, aging, and strong light. It has the property of being easy. Therefore, by providing the food and drink with the lutein degradation inhibitor together with lutein, it is possible to prevent the deterioration of lutein due to external factors as described above, and to provide an excellent food and drink that can further maintain the effect of lutein. It becomes possible to do.
In addition, there is no restriction | limiting in particular as lutein which can be contained in the said food / beverage products, For example, it can obtain by extracting from marigold (food additive "marigold pigment | dye").

There is no restriction | limiting in particular as said other component, According to the objective, it can select suitably, For example, the auxiliary | assistant raw material or additive etc. which are normally used in manufacturing the said food / beverage products are mentioned.
The auxiliary raw material or additive is not particularly limited and may be appropriately selected depending on the intended purpose. For example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid , Tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, Arabia Examples include gum, carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives and the like.

There is no restriction | limiting in particular as content of the said lutein degradation inhibitor in the said food / beverage products, Although it can select suitably according to the objective, For example, a turrengue with respect to the total amount (100 mass%) of food / beverage products The amount of the extract is preferably 0.001% by mass to 50% by mass, and more preferably 0.01% by mass to 20% by mass.
Moreover, there is no restriction | limiting in particular as content of the said lutein degradation inhibitor with respect to content of lutein in the said food-drinks, Although it can select suitably according to the objective, For example, content (100 mass of lutein) Part)), the amount of the extract of vines is preferably 100 parts by weight to 10,000 parts by weight, more preferably 500 parts by weight to 5,000 parts by weight.

(effect)
The lutein degradation preventive agent of the present invention and the food and drink of the present invention can be taken orally on a daily basis, and the action of the extract of turrengue, which is an active ingredient, prevents lutein degradation, in particular, The effect of preventing light deterioration can be exhibited extremely effectively. Therefore, the lutein deterioration preventing agent of the present invention and the food and drink of the present invention are, for example, health foods and nutrients containing lutein for the purpose of treatment or prevention of eye function enhancement, cataracts, age-related macular degeneration, etc. It is considered that it can be widely used for supplements.
In addition, although the lutein degradation inhibitor of this invention and the food-drinks of this invention are suitably applied with respect to a human, as long as the effect is show | played, animals other than a human (for example, mouse | mouth) , Rat, hamster, dog, cat, cow, pig, monkey, etc.). Moreover, the lutein degradation inhibitor of this invention and the food-drinks of this invention use the extract of naturally-derived turrengue as an active ingredient, and are advantageous also at the point which is excellent in safety | security.

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

(Production Example 1: Manufacture of water extract of vines)
As a raw material for extraction, 1,000 g of pulverized seeds of crane were added to 5,000 mL of water, kept at 90 ° C. for 2 hours with stirring, and then filtered. The filtrate was concentrated under reduced pressure at 60 ° C. and further dried with a vacuum dryer to obtain an extract (powder). The yield of the obtained extract is shown in Table 1.

(Production Example 2: Production of 30% by mass ethanol extract of vines)
As a raw material for extraction, 1,000 g of vine seeds were added to 5,000 mL of 30 mass% ethanol (mass ratio of water and ethanol 7: 3), kept under reflux for 2 hours with stirring, and then filtered. The filtrate was concentrated under reduced pressure at 60 ° C. and further dried with a vacuum dryer to obtain an extract (powder). The yield of the obtained extract is shown in Table 1.

(Production Example 3: Manufacture of 70% by weight ethanol extract of Tsururenge)
As a raw material for extraction, 1,000 g of vine seeds were put into 5,000 mL of 70 mass% ethanol (mass ratio of water and ethanol 3: 7), kept under reflux for 2 hours with stirring, and then filtered. The filtrate was concentrated under reduced pressure at 60 ° C. and further dried with a vacuum dryer to obtain an extract (powder). The yield of the obtained extract is shown in Table 1.

(Example 1: Test for preventing light degradation of lutein)
Using each of the perilla extract of Production Examples 1 to 3 as a test sample, the effect of preventing the photodegradation of lutein of the pertussis extract was tested by the following test method.

Lutein (Wako Pure Chemical Industries, Ltd.) is dispersed in ethanol and dissolved in a buffer solution (PBS Ca ²⁺ , Mg ²⁺ free) so that the lutein content is 0.08 mg / mL. did. Each turrengue extract is added to the lutein solution so as to have a concentration of 2 mg / mL, 1 mg / mL, and 0.5 mg / mL, and left in a growth chamber set at a temperature of 37 ° C. and an illuminance of 15,000 lx. For the solution diluted 20 times with PBS, the absorbance at 447 nm showing the maximum absorption was measured. When the absorbance on the 0th day after standing was defined as 100%, the ratio of the absorbance on the 1st to 8th days after standing was evaluated as the lutein residual rate (%). The results are shown in Table 2.

  From the results of Table 2, in the case of lutein alone, the deterioration of lutein gradually progressed after standing, and on the 8th day, the residual rate of lutein was reduced to about 50%, whereas turrengue 30% EtOH extraction When the product is added, the rate of deterioration is suppressed at all added concentrations, and the residual rate of lutein is suppressed to about 70% to 80% even at 8 days after standing, with about 20% to 30% deterioration. I understand that.

  As described above, from the results of Example 1 above, it was confirmed that the Turrengue extract has a strong action of preventing deterioration of lutein due to light. Therefore, the vine extract is very useful as a lutein degradation preventive agent, and is a health food and nutritional supplement containing lutein for the purpose of treatment or prevention of eye function enhancement, cataracts, age-related macular degeneration, etc. It was suggested that it can be widely used for foods.

(Formulation Example 1: Tablet-shaped dietary supplement)
The following mixture was tableted to produce a tablet-shaped dietary supplement.
・ Tsurengage 30% by mass ethanol extract of Production Example 2 ... 10% by mass
・ Lutein: 1% by mass
・ Powdered sugar (sucrose): 52% by mass
・ Guar gum decomposition product: 33% by mass
・ Glycerin fatty acid ester: 4% by mass

(Formulation Example 2: Granular dietary supplement)
The following mixture was formed into granules to produce a dietary supplement.
・ Village water extract of Production Example 1 6.2 mass%
・ Lutein: 0.5% by mass
・ Beat oligosaccharide: 59.3 mass%
・ Vitamin C: 33.3% by mass
-Stevia extract: 0.7% by mass

(Formulation Example 3: Soft capsule dietary supplement)
The following mixture was filled into soft capsules to produce a dietary supplement.
・ Tsurengage 30% by mass ethanol extract of Production Example 2 33% by mass
・ Lutein: 5% by mass
・ Vegetable oil: 38% by mass
・ Glycerin fatty acid ester: 4% by mass
・ Beeswax: 20% by mass

  Since the lutein deterioration preventing agent of the present invention contains an extract of turrengue, it has an excellent anti-degradation effect of lutein and is also excellent in safety. Therefore, the lutein degradation preventing agent of the present invention can be widely used for health foods and nutritional supplements containing lutein for the purpose of, for example, strengthening the function of the eye and treating or preventing cataracts, age-related macular degeneration, etc. is there.

Claims (4)

A lutein degradation inhibitor comprising an extract of Astragalus complanatus R. Br.   The lutein deterioration preventive agent according to claim 1, which has an effect of preventing the light deterioration of lutein.   A food or drink comprising the lutein degradation inhibitor according to any one of claims 1 to 2.   Furthermore, the food-drinks of Claim 3 containing a lutein.