JP2009102242A - Skin-lightening agent, and external preparation for skin-lightening - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new skin-lightening agent useful as an external preparation for skin, and the like, and to provide an external preparation for skin-lightening, exhibiting an excellent skin-lightening effect. <P>SOLUTION: A skin-lightening agent contains, as an active ingredient, an extract of fermented amaranth obtained by extracting a fermentation product obtained by fermenting amaranth fruits with a solvent containing at least an alcohol, and the external preparation for lightening the skin, contains the skin-lightening agent. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a whitening agent obtained by extraction from a fermentation product obtained by fermenting amaranth fruit, and a skin whitening preparation containing the same.

Conventionally, it has been found that an extract of amaranthus has various medicinal effects, and it has been proposed to be blended into cosmetics, foods and the like (for example, Patent Documents 1 to 4).
JP 10-330242 A JP-A-11-255612 JP 2000-143524 A JP 2006-111580 A

However, a higher medicinal effect is demanded for components blended in a skin external preparation such as cosmetics.
An object of the present invention is to provide a novel whitening agent useful as a medicinal agent such as an external preparation for skin.
Moreover, this invention makes it a subject to provide the skin external preparation for whitening which shows the outstanding whitening effect.

In order to solve the above-mentioned problems, the present invention provides a whitening agent comprising, as an active ingredient, an amaranth fermentation extract extracted from a fermentation product obtained by fermenting an amaranth fruit with a solvent containing at least an alcohol.
As an aspect of the present invention, the whitening agent, wherein the fermentation product is a fermentation product obtained by allowing a fungus belonging to the genus Aspergillus to act and ferment; the filamentous fungus belonging to the genus Aspergillus is Aspergillus The whitening agent that is oryzae or Aspergillus soya; the whitening agent in which the fermentation product is a fermentation product fermented by a solid culture method; and the alcohol concentration in the solvent is 50 to 100% by volume. Said whitening agent.

  Further, from another viewpoint, according to the present invention, a skin whitening external preparation for skin whitening containing the fermentation product; a maranthus fermentation extract extracted from a fermentation product obtained by fermenting amaranth fruit, with a solvent containing at least alcohol A method for producing a whitening skin external preparation comprising adding a product; and a method for producing a whitening agent comprising fermenting amaranth fruit.

ADVANTAGE OF THE INVENTION According to this invention, the novel whitening agent useful as medicinal agents, such as a skin external preparation, can be provided.
Moreover, according to this invention, the skin external preparation for whitening which shows the outstanding whitening effect can be provided.

Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.
The present invention relates to a whitening agent containing, as an active ingredient, an amaranth fermentation extract extracted from a fermentation product obtained by fermenting an amaranth fruit, using a solvent containing at least an alcohol.
Amaranth (English name: Amaranth) is a plant belonging to the Amaranthaceae family. In the present invention, the amaranth fruit used as a raw material is not particularly limited. Various products such as those marketed for food and those marketed for feed and food processing raw materials can be used.
Further, the amaranth fruit may be used as a raw material as it is, or may be used after being pulverized and granulated in order to increase the efficiency of the fermentation process.

  At least one microorganism is used to ferment the amaranth fruit. The microorganism used is not particularly limited as long as safety is guaranteed. Any of filamentous fungi (such as Koji fungi) and bacteria (such as yeast, Bacillus subtilis and lactic acid bacteria) can be used, and one or more of these strains can be selected and used. Among these microorganisms, fungi used in food production are preferable, and fungi belonging to the genus Aspergillus, Penicillium, Rhizopus, Monascus and the like are preferable. Further, among these bacteria, Aspergillus oryzae, Aspergillus niger, Aspergillus soya and the like classified into Koji fungi are particularly preferable. In addition, as the above-mentioned microorganism, a mutant strain microbially mutated can be used naturally or by an artificial means utilizing a chemical substance such as nitrosoguanidine, X-rays or ultraviolet rays.

  In this invention, there is no restriction | limiting in particular about the state and origin of the microorganisms used for fermentation. For example, microbial cells or spores stored in a slant medium or the like can be used. Moreover, you may use what was obtained by performing preculture with a preferable culture medium, for example, a YPD culture medium etc. previously. Moreover, you may use the microbial cell collect | recovered after culture | cultivating another suitable culture medium. Furthermore, you may use the koji manufactured through the manufacturing process of koji generally performed by sake and miso manufacture in that state.

  In the present invention, the fermentation of amaranth fruit can be performed by any of a solid culture method in which microorganisms are grown on a solid substrate and the fermentation proceeds, and a liquid culture method in which microorganisms are grown in a liquid medium and the fermentation is advanced. You may use it. It is preferable to proceed by a solid culture method because an amaranth fermentation extract exhibiting higher efficacy, particularly higher whitening effect can be obtained. The solid culture method is a method used for making koji in sake and miso manufacturing. In the present invention, it is preferable to utilize a medium containing amaranth fruit, inoculate the medium by adding a pre-cultured fungus, and allow fermentation to proceed by a solid culture method. The preculture can be performed, for example, by inoculating a liquid medium from a slant medium, and the culture solution obtained by the preculture can be added to the medium as it is. Moreover, it is preferable to sterilize the medium containing the amaranth fruit before inoculating the pre-cultured bacteria.

  The medium may be supplied with nutrients necessary for the growth of microorganisms. Although preferable examples of the nutrient source to be supplied vary depending on the microorganism to be used, in general, full course and starch are used as the carbon source, and peptone, yeast extract and the like are used as the nitrogen source. However, in the present invention, since amaranth fruit can be a good nutrient source, the addition of other nutrient sources is better.

  Fermentation conditions, specifically, the temperature / humidity when the fermentation is in progress, and the fermentation period, etc., should be selected so as to be preferable conditions with reference to the conventional fermentation treatment using Aspergillus oryzae. Good. As an example of preferable conditions for the solid culture method, the water content of the medium containing the amaranth fruit is about 60% by mass, the culture temperature is about 30 ° C., and the culture period is 2 to 8 days (more preferably 3 to 6 days). It is.

  In the present invention, as described above, it is preferable to obtain a fermentation product using a solid culture method, but of course, a fermentation product may be obtained using a liquid culture method. In the liquid culture method, for example, an amaranth fruit extract (extract obtained by extraction with water, hot water, hydrous alcohol, etc. by a conventional method) is included in the medium, and the fermentation proceeds. Can be made. As the method, various culture methods such as a shaking culture method, a stationary culture method, and a tank culture method can be used.

  In the solid culture method, since the fermentation product is obtained in a state in which the amaranth fruit and the koji fungus that are the medium are integrated, it is preferable that the amaranth fruit and fungus used as the medium are also subjected to an extraction treatment. For example, after completion of the solid culture, an amaranth fruit that is used as a medium and to which a koji fungus has adhered can be taken out and further subjected to a pulverization treatment, if desired, followed by an extraction treatment. On the other hand, in the liquid culture method, an extraction process is performed on a culture solution generated by fermentation.

  The solvent used for the extraction treatment contains at least alcohol. Examples of alcohols include lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.) and liquid polyhydric alcohols (glycerin, propylene glycol, 1,3- Any of butylene glycol etc.) is included, but among these, lower monohydric alcohols are preferred. The said solvent may contain 2 or more types of alcohol. Moreover, the said solvent may contain 1 or more types of water other than alcohol, for example, and another organic solvent. Examples of other organic solvents include lower esters (such as ethyl acetate). The solvent preferably contains 10 to 100% by volume of alcohol, and more preferably contains 50 to 100% by volume. Among them, a mixed solvent of alcohol and water is preferable, and a mixed solvent of alcohol and water, and a solvent containing alcohol in the above ratio is more preferable. The water that can be mixed with alcohol may be a buffer solution with adjusted pH. Moreover, you may perform a heating and stirring at the time of an extraction process.

After the extraction treatment, solids such as amaranth fruit used as a medium can be removed from the extract by centrifugation, filtration or the like. Furthermore, if desired, steps such as purification, decolorization, and deodorization can be performed. The purification can be performed using a column such as an adsorption resin. Deodorization and decolorization can be performed using activated carbon. The extract may be prepared as a solid such as a powder by concentrating and drying by various methods according to the intended use. For example, it can be concentrated by vacuum concentration, heat concentration, vacuum concentration or the like. Further, for example, it can be dried by spray drying, freeze drying or the like.
In addition, treatments such as purification, concentration, and drying can be carried out by appropriately selecting methods and the like within a range that does not impair the effectiveness of the obtained amaranth fermentation extract.

  The amaranth fermentation extract used as an active ingredient in the whitening agent of the present invention may be in any form such as liquid, paste or gel. The liquid extract containing the extraction solvent can also be used after it is solidified by drying under reduced pressure or freeze drying. It can also be dried by spray drying or the like and used as a powder.

  An extract of a fermentation product obtained by fermenting amaranth fruit with a predetermined solvent contains various components different from the unfermented extract such as amaranth fruit, and has a different effect and / or more than conventional. Excellent efficacy.

The whitening agent of this invention which uses the said amaranth fermentation extract as an active ingredient shows the high whitening effect, and can be used as a medicinal agent of a skin external preparation. The skin external preparation containing the whitening agent of the present invention can impart a whitening effect to the skin when applied to the skin by the whitening action of the amaranth fermentation extract.
Hereinafter, the skin external preparation for whitening containing the whitening agent of this invention is demonstrated.

  The skin external preparation for whitening of this invention is a skin external preparation containing the whitening agent of this invention. Although there is no restriction | limiting in particular about the compounding quantity of the said amaranth fermentation extract used as a whitening agent, The said amaranth fermentation extract is 0.0001-as solid content with respect to the total mass of the skin external preparation for whitening which is a final form. It is preferably 5% by mass (hereinafter simply referred to as “%”), more preferably 0.001 to 3%. If it exists in this range, this extract can be mix | blended stably, brings about a high whitening effect, and can exhibit the high whitening effect based on it.

  In the skin external preparation for whitening, other active ingredients are blended within the range that does not impair the effects of the present invention, for example, other active ingredients that exhibit whitening effect, anti-aging effect, or wrinkle formation suppression effect by exposure to ultraviolet rays, etc. May be. More specifically, examples include, but are not limited to, UV protection agents, antibacterial agents, other whitening agents, anti-inflammatory agents, active oxygen removing agents, and moisturizing agents.

  In addition, in the above-mentioned skin external preparation, components that are usually used in the production of cosmetics, quasi-drugs, skin external preparations and the like, for example, water (purified water, hot spring water, Deep water etc.), oil agent, surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film forming agent, resin, inclusion compound, antibacterial agent, fragrance, deodorant, salt, pH adjuster, freshener, plant / animal / microbe extract, active oxygen remover, blood circulation promoter, astringent, antiseborrheic agent, moisturizer, chelating agent, keratolytic agent, enzyme, hormones, etc. Vitamins and the like can be added as necessary.

  The external preparation for skin includes powders such as powder and powder foundation; solids such as soap and lipstick; emulsions such as cream, milky lotion and cream foundation; liquids such as skin lotion and cosmetic liquid; Preferably, the composition is a material composition. Further, it may be a shampoo, a treatment, or a skin external preparation for scalp, but is not limited thereto.

EXAMPLES Hereinafter, although an Example demonstrates this invention further more concretely, the scope of the present invention is not limited to the following Example.
[Example 1: Preparation of Amaranthus Fermentation Extract Samples 1 and 2]
・ Preparation of amaranth fermentation extract sample 1 (pre-culture)
Glucose 1%, yeast extract 0.5% and water 98.5% were mixed and further adjusted to pH 5.4 to obtain a medium (preculture medium) used for preculture. 100 mL of this preculture medium was put into an Erlenmeyer flask with a baffle (500 mL), and sterilized at 121 ° C. for 20 minutes. Thereafter, spores of Aspergillus oryzae TOYO-007 were removed from the PDA slant medium for storage and inoculated into the pre-culture medium subjected to the above-described sterilization. Subsequently, the cells were cultured for 24 hours in an environment of a rotation speed of 140 rpm and a temperature of 30 ° C. using a shaking culture apparatus.

(Main culture)
10 g of amaranth fruit was placed in an Erlenmeyer flask (300 mL) and sterilized at 121 ° C. for 30 minutes. A total of 4 samples were prepared and used as an amaranth medium for solid culture. Inoculation was performed by adding 6 mL of the precultured medium cultured in the above-described preculture to an amaranth medium and mixing with a separately sterilized spatula so that the precultured liquid becomes uniform. Next, static culture was performed for 4 days in an environment of 30 ° C. using an incubator.

(Extraction)
After completion of the culture, about 50 g of the culture was taken out from the Erlenmeyer flask together with the medium, 50 mL of water was added, and homogenized with a food mill to prepare a culture paste. Extraction was further performed on 50 g of this paste using 200 mL of two types of water-ethanol mixed solvents having different mixing ratios. The sample extracted using a solvent having a water / ethanol mixing ratio of 25/75% by volume was an amaranth fermentation extract sample 1; and a solvent having a water / ethanol mixing ratio of 50/50% by volume. The extracted sample was Amaranthus Fermentation Extract Sample 2.
Thereafter, the extract is centrifuged (7,000 rpm, 30 minutes), further filtered through a filter paper (5C), solid-liquid separation is performed, and 180 mL of Amaranthus fermented extract samples 1 and 2 are obtained as the extract. It was.
Moreover, about 6.2 g of powders of amaranth fermentation extract samples 1 and 2 were obtained by drying this extract by freeze-drying.

[Reference Example 1: Preparation of Amaranthus Fermented Extract Sample 3]
・ Preparation of Amaranthus Fermentation Extract Sample 3 The number of days of main culture was changed to 3 days, and after completion of the culture, water was added without adding any alcohol, followed by hot water extraction (heating at 100 ° C. for 30 minutes). Amaranthus fermented extract sample 3 was obtained in the same manner as in Example 1 except that extraction was performed.

[Preparation of Amaranthus Unfermented Extract Sample 4 for Comparative Example]
As a sample for a comparative example, the amaranth fruit was sterilized by autoclave (121 ° C., 30 minutes) and then heated at 100 ° C. for 30 minutes to perform hot water extraction. Thereafter, the extract obtained by solid-liquid separation was freeze-dried to obtain an amaranth unfermented extract sample 4.

[Example 2: Inhibition of melanin production (whitening effect) test]
Appropriate amounts of medium are collected in two 6-well petri dishes, seeded with mouse-derived B16 melanoma cells, and allowed to stand at 37 ° C. in a carbon dioxide concentration of 5%. The next day, the prepared solutions of each sample (Amaranthus fermented extract samples 1 to 3 and Amaranthus unfermented extract sample 4) are added and mixed so that the final concentration is the concentration shown in the following table. On the fifth day of culture, the medium is changed, and the sample preparation solution is added again. On the next day, the medium was removed and the cells were collected after washing with a phosphate buffer in one petri dish, and the degree of whiteness of the cultured B16 melanoma cells was evaluated visually according to the following criteria.
In the following criteria, “control” means cells cultured in the same manner without addition of a sample.
(Criteria)
++: Extremely white relative to the control.
+: Clearly white with respect to the control.
±: Slightly white with respect to the control.
-: The same black color as the control.

  The remaining one petri dish was stained with formalin-fixed cells after adding 1% crystal violet solution. The number of viable cells (A) and the number of control cells (B) for each analyte concentration were measured with a monocellator. The above results are shown in the table.

From the above results, it can be understood that both of the amaranth fermentation extract samples 1 and 2 extracted using a solvent having an alcohol concentration of 50% by volume or more have a melanin-inhibiting action. In addition, when a melanin suppression test similar to the above is performed on a conventionally used whitening agent, considering that an evaluation result of “±” or more is obtained, the amaranth fermentation extract samples 1 and 2 (especially sample 1) are It can be understood that it exhibits an excellent whitening effect and is useful as a whitening agent.
On the other hand, the amaranth fermented extract sample 3 (reference example) prepared by performing extraction using only water and the amaranth unfermented extract sample 4 (comparative example) prepared by extracting unfermented amaranth with water. ) Showed no whitening effect.

[Example 3: Preparation of cleansing cream]
A cleansing cream having the following composition was prepared by the following method.
(Ingredient) (%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 3.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Beeswax 1.5
(5) Vaseline 6.0
(6) Liquid paraffin 40.0
(7) Dimethylpolysiloxane (100CS) 0.5
(8) Sorbitan sesquioleate 1.0
(9) Preservative appropriate amount (10) Triethanolamine 1.0
(11) Propylene glycol 10.0
(12) Polyethylene glycol 20000 0.5
(13) Carboxyvinyl polymer 1% aqueous solution 5.0
(14) Purified water Residual amount (15) Amaranth fermentation extract * 1 0.1
(16) Perfume appropriate amount * 1 Sample 2 prepared in Example 1

(Manufacturing method)
A. Ingredients (1) to (9) are dissolved by heating and maintained at 70 ° C.
B. Ingredients (10) to (14) are dissolved by heating and maintained at 70 ° C.
C. A is added to B and emulsified.
D. After cooling C, components (15) and (16) were added and mixed to obtain a cleansing cream.

  The prepared cleansing cream is a cleansing cream that is well-familiar with make-up stains, cleans the make-up stains, has moderate moisture and emollient on the skin, has no stickiness, and provides a whitening effect when used daily. .

[Example 4: Preparation of face wash]
A face wash having the following composition was produced by the following method.
(Ingredient)%
(1) Lauric acid 5.0
(2) Myristic acid 18.5
(3) Stearic acid 6.0
(4) Glycerin 12.0
(5) Polyethylene glycol 1500 5.0
(6) Potassium hydroxide 6.5
(7) Purified water remaining amount (8) Palm oil fatty acid diethanolamide 5.0
(9) Palm oil fatty acid methyl taurine sodium 1.8
(10) Polyoxyethylene (7.5E.O.) lauryl ether 2.0
(11) Ethylene glycol distearate 1.0
(12) Hydroxypropyl methylcellulose 1% aqueous solution 5.0
(13) Amaranth fermentation extract 0.1
(14) Perfume appropriate amount * 1 Sample 1 prepared in Example 1

(Manufacturing method)
A. Components (1) to (5) are dissolved by heating.
B. Components (6) and (7) are dissolved by heating.
C. Components (8) to (11) are dissolved by heating.
D. Add B to A and mix, then add C and mix.
E. After cooling D, components (12), (13) and (14) were added and mixed to obtain a face wash.

  The prepared facial cleanser was rich and rich in foaming, cleansed well, washed cleanly, left moisturized on the skin, and provided a whitening effect by daily use.

[Example 5: Preparation of lotion 1 (dissolving type)]
A lotion having the following composition was prepared by the following method.
(Ingredient)%
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) aqueous solution 0.5
(4) Amaranth fermentation extract * 1 0.05
(5) Glycerin 3.0
(6) 1,3-butylene glycol 8.0
(7) Purified water remaining amount (8) Ethyl alcohol 10.0
(9) Perfume Appropriate amount (10) Preservative Appropriate amount (11) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
* 1 Sample 1 prepared in Example 1

(Manufacturing method)
A. Components (1) to (7) are mixed and dissolved.
B. Components (8) to (11) are mixed and dissolved.
C. B was added to A and mixed to obtain a skin lotion.

  The prepared lotion has a fresh feeling of use, is excellent in moisture, and can be applied to the skin every day to make the texture smooth and glossy. It was a lotion that gave an effect.

[Example 6: Preparation of lotion 2 (oil-solubilized type)]
A lotion having the following composition was prepared by the following method.
(Ingredient)%
(1) Meadow Home Oil 0.05
(2) Jojoba oil 0.05
(3) Perfume appropriate amount (4) preservative appropriate amount (5) polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(6) Isostearic acid polyoxyethylene hydrogenated castor oil (50 EO) 1.0
(7) Ethyl alcohol 8.0
(8) Glycerin 5.0
(9) 1,3-butylene glycol 5.0
(10) Polyethylene glycol 1500 0.1
(11) Amaranth fermentation extract * 1 0.2
(12) Purified water remaining amount * 1 Sample 2 prepared in Example 1

(Manufacturing method)
A. Components (1) to (7) are mixed and dissolved.
B. Components (8) to (12) are mixed and dissolved.
C. A is added to B and mixed to obtain a skin lotion.

  The prepared lotion has a smooth feeling of use, is excellent in moisture, and can be applied to the skin every day to make the texture smooth. It was a lotion that gave a whitening effect.

[Example 7: Preparation of lotion 3 (emulsification type)]
A lotion having the following composition was prepared by the following method.
(Ingredient)%
(1) Glyceryl tri-2-ethylhexanoate 0.08
(2) Squalane 0.02
(3) Sorbitan sesquioleate 0.05
(4) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.05
(5) Polyoxyethylene (8E.O.) alkyl (12-15)
Ether phosphoric acid 0.1
(6) Preservative appropriate amount (7) perfume appropriate amount (8) ethyl alcohol 8.0
(9) Dipropylene glycol 8.0
(10) Glycerin 4.0
(11) Amaranth fermentation extract * 1 0.1
(12) Purified water remaining amount * 1 Sample 2 prepared in Example 1

(Manufacturing method)
A. Components (1) to (8) are mixed and dissolved.
B. Components (9) to (12) are mixed and dissolved.
C. A is added to B and emulsified to obtain a skin lotion.

  The prepared lotion has a fresh and refreshing feeling of use, is excellent in moisture of the skin, and can be applied to the skin every day, making it smooth and glossy, making it beautiful and beautiful skin, It was a lotion that gave whitening effect by daily use.

[Example 8: Preparation of emulsion]
An emulsion having the following composition was prepared by the following method.
(Ingredient)%
(1) Stearic acid 1.0
(2) Cetanol 0.5
(3) Lipophilic glyceryl monostearate 0.5
(4) Liquid paraffin 2.0
(5) Squalane 3.0
(6) Jojoba oil 3.0
(7) Cetyl palmitate 0.2
(8) Preservative appropriate amount (9) Sorbitan monostearate 0.3
(10) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(11) Triethanolamine 0.5
(12) 1,3-butylene glycol 15.0
(13) Glycerin 3.0
(14) Polyethylene glycol 6000 0.5
(15) Purified water Remaining amount (16) Carboxyvinyl polymer 1% aqueous solution 8.0
(17) Amaranth fermentation extract * 1 0.2
(18) Perfume appropriate amount * 1 Sample 1 prepared in Example 1

(Manufacturing method)
A. Ingredients (1) to (10) are dissolved by heating and maintained at 70 ° C.
B. Ingredients (11) to (15) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and component (16) is further added and mixed.
D. C is cooled, and components (17) and (18) are added and mixed to obtain an emulsion.

  The prepared emulsion has a fresh and smooth feeling of use, is excellent in moisture and emollient, and can be applied to the skin every day to make the texture smooth and glossy. It was an emulsion that gave a whitening effect by daily use.

Example 9: Preparation of cream
The following compositional claims were prepared in the following manner.
(Ingredient)%
(1) Stearic acid 2.5
(2) Cetanol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Vaseline 2.0
(5) Dipentaerythritol fatty acid ester * 1 2.0
(6) Isotridecyl myristate 5. O
(7) Liquid paraffin 8.0
(8) Squalane 5.0
(9) Beeswax 1.0
(10) Cetyl palmitate 2.0
(11) Sorbitan sesquioleate 0.5
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.5
(13) Preservative appropriate amount (14) Triethanolamine 1.2
(15) 1,3-butylene glycol 8.0
(16) Glycerin 2.0
(17) Polyethylene glycol 20000 0.5
(18) Purified water remaining amount (19) 1% aqueous solution of carboxyvinyl polymer 5.0
(20) Amaranth fermentation extract * 2 1.0
(21) Fragrance appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2 Sample 1 prepared in Example 1

(Manufacturing method)
A. Ingredients (1) to (13) are dissolved by heating and maintained at 70 ° C.
B. Ingredients (14) to (18) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and component (19) is further added and mixed.
D. C is cooled, and components (20) and (21) are added and mixed to obtain an emulsion.

  The prepared cream has a rich and smooth feel, excellent emollient skin, and when applied to the skin every day, it can be textured and glossy, with beautiful and elastic skin. It was a cream that gave a whitening effect.

[Example 10: Preparation of serum (solubilized type)]
A serum having the following composition was prepared by the following method.
(Ingredient)%
(1) Glyceryl tri-2-ethylhexanoate 0.1
(2) Meadow Home Oil 0.05
(3) Jojoba oil 0.05
(4) Preservative appropriate amount (5) perfume appropriate amount (6) polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(7) Polyoxyethylene hydrogenated isostearate castor oil (50 EO) 1.5
(8) Ethyl alcohol 5.0
(9) Glycerin 4.0
(10) Dipropylene glycol 8.0
(11) 1,3-butylene glycol 8.0
(12) Sodium lactate 0.5
(13) Sodium pyrrolidonecarboxylate (50%) aqueous solution 0.5
(14) Hydroxyethyl cellulose 0.08
(15) Sodium alginate 0.05
(16) Amaranth fermentation extract * 1 0.1
(17) Purified water remaining amount * 1 Sample 1 prepared in Example 1

(Manufacturing method)
A. Components (1) to (8) are mixed and dissolved.
B. Components (9) to (17) are mixed and dissolved.
C. A was added to B and mixed to obtain a serum.

  The prepared essence has a fresh, smooth and mild feeling of use, is excellent in moisture and emollient skin, and is applied to the skin every day to make it smooth and glossy. It was a serum that can give a whitening effect by daily use.

[Example 11: Preparation of pack (peel-off type)]
A pack having the following composition was prepared by the following method.
(Ingredient)%
(1) Polyvinyl alcohol 12.0
(2) Methylcellulose 0.1
(3) Glycerin 3.0
(4) 1,3-butylene glycol 5.0
(5) Purified water remaining amount (6) perfume appropriate amount (7) preservative appropriate amount (8) glyceryl tri-2-ethylhexanoate 0.1
(9) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.0
(10) Ethyl alcohol 13.0
(11) Amaranth fermentation extract * 1 0.1
* 1 Sample 2 prepared in Example 1

(Manufacturing method)
A. Components (1) to (5) are dissolved by heating.
B. Components (6) to (10) are mixed and dissolved.
C. After cooling A, B and (11) were added and mixed to obtain a pack.

  The prepared pack is easy to stretch, gives moderate tension to the skin, does not feel sticky on the skin after peeling the pack, has a high moisture and weight, and gives a whitening effect by daily use. there were.

[Example 12: Preparation of massage cream]
A massage cream having the following composition was prepared by the following method.
(Ingredient)%
(1) Stearic acid 2.0
(2) Stearyl alcohol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Sorbitan sesquioleate 1.0
(5) Cetyl palmitate 1.0
(6) Dipentaerythritol fatty acid ester * 1 4.0
(7) Vaseline 20.0
(8) Liquid paraffin 28.0
(9) Methyl polysiloxane (100CS) 0.5
(10) Sodium hydroxide 0.1
(11) Dipropylene glycol 7.0
(12) Carboxyvinyl polymer 1% aqueous solution 5.0
(13) Purified water Residual amount (14) Amaranth fermentation extract * 2 0.2
(15) Fragrance appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2 Sample 1 prepared in Example 1

(Manufacturing method)
A. Ingredients (1) to (9) are dissolved by heating and maintained at 70 ° C.
B. Components (10) to (13) are dissolved by heating and maintained at 70 ° C.
C. A is added to B and emulsified.
D. After cooling C, components (14) and (15) were added and mixed to obtain a massage cream.

  The prepared massage cream is a massage cream that has a smooth and rich feeling of use, has an excellent massage effect, and has a high effect of preventing a decrease in elasticity, as well as a whitening effect by daily use.

[Example 13: Preparation of cream foundation (W / O type)]
A cream foundation having the following composition was prepared by the following method.
(Ingredient)%
(1) Sericite 8.0
(2) Titanium oxide 10.0
(3) Bengala 1.0
(4) Yellow iron oxide 2.2
(5) Mica titanium 2.0
(6) Black iron oxide 0.1
(7) Organic metamorphic B-tonite * 1 0.5
(8) Long-chain alkyl-containing polyoxyalkylene modified organopolysiloxane * 2 2.0
(9) Polyoxyalkylene-containing organopolysiloxane * 3 2.0
(10) Decamethylcyclopentasiloxane 12.0
(11) Octamethylcyclotetrasiloxane 10.0
(12) Trimethylsiloxysilicic acid silicone solution * 4 3.0
(13) Glyceryl tri-2-ethylhexanoate 5.0
(14) 1,3-butylene glycol 5.0
(15) Diglycerin 2.0
(16) Glycerin 2.0
(17) Xanthan gum 0.2
(18) Sodium chloride 0.2
(19) Purified water remaining amount (20) Amaranth fermentation extract * 5 0.1
(21) Perfume appropriate amount * 1: Benton 38 (manufactured by NL Endowry)
* 2: Avil EM-90 (Gold Schmidt)
* 3: Silicone KF6017 (manufactured by Shin-Etsu Chemical Co., Ltd.)
* 4: Silicone KF7312J (manufactured by Shin-Etsu Chemical Co., Ltd.)
* 5: Sample 1 prepared in Example 1

(Manufacturing method)
A: Components (8) to (13) are mixed.
B: Components (14) to (19) are mixed.
C: Components (1) to (7) are added to A and mixed and dispersed.
D: Components (20) and (21) were added to C to obtain a cream foundation (W / O type).

  The prepared cream foundation has a smooth feel without stickiness, excellent adhesion to the skin, and a beautiful finish.When applied to the skin every day, it gives the skin an emollient feeling that is also elastic. It was a cream foundation that can make skin beautiful and give a whitening effect by daily use.

ADVANTAGE OF THE INVENTION According to this invention, the novel whitening agent useful for skin external preparations etc. can be provided.
Moreover, according to this invention, the skin external preparation for whitening which shows the outstanding whitening effect can be provided.

Claims (6)

A whitening agent comprising, as an active ingredient, an amaranth fermentation extract extracted from a fermentation product obtained by fermenting amaranth fruit with a solvent containing at least alcohol. The whitening agent according to claim 1, wherein the fermentation product is a fermentation product obtained by allowing a fungus belonging to the genus Aspergillus to act and ferment. The whitening agent according to claim 2, wherein the filamentous fungi belonging to the genus Aspergillus are Aspergillus oryzae and Aspergillus soja which are Koji fungi. The whitening agent according to any one of claims 1 to 3, wherein the fermentation product is a fermentation product fermented by a solid culture method. The whitening agent according to any one of claims 1 to 4, wherein an alcohol concentration in the solvent is 50 to 100% by volume. The skin external preparation for whitening containing the amaranth fermentation extract in any one of Claims 1-5.