JP2008247863A - Attractant sustainedly releasing sex pheromone of trigonotylus caelestialium - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To improve components of the attractant so that the attractant exhibits stable attracting performances and to make it possible to release the improved pheromone into the air stably for long terms. <P>SOLUTION: n-Hexyl acetate is added as a fourth component to n-hexyl n-caproate, 2-hexenyl n-caproate (E) and n-octyl n-butyrate which are known as attractive sex pheromones. Thus, the enhancement of the attracting performances is confirmed. The sex pheromone is mixed with a sustainedly releasing resin 2 and is charged in a container 1 equipped with an opening 3 in order to make it possible to release the sex pheromone stably for long terms. Mixing of the resin with the sex pheromone permits release of the sex pheromone into the air with volatilization from the resin. The amount of the released sex pheromone can be controlled by the releasing speed from the sustainedly releasing resin, control of release by the permeable container or the diameter of the opening of the hardly permeable container to assure stable results. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an attractant preparation using a sex pheromone of a stink bug, Trichonotylus caelestialium (Kirkaldy), an important pest that causes damage to spotted rice of rice.

  In order to suppress the damage to rice crops caused by stink bugs, it is effective to monitor the occurrence of adult worms (monitoring) and implement appropriate control with pesticides. For this reason, conventionally, investigation of capture length during the cultivation period has been carried out by a scooping method using a catching net or a preliminary light. However, these methods require a large amount of research effort, and skill is required to select and discriminate captured insects.In addition, the scooping method involves fluctuations in capture efficiency due to weather factors such as rain and wind, and individual differences in survey accuracy. Due to its large size, it is not an easy survey method. Therefore, pheromone traps using sex pheromones, which are species-specific attractants, are expected to be put to practical use as a monitoring method that anyone can easily perform with the same accuracy.

  At the 2000 Hokkaido Agricultural Experiment Conference (January 2001), 17 EAD active substances in the extract of female stink bugs (including substance identification of the fourth component of the present invention, but the attraction activity is unknown). Of the two main components of sex pheromone (n-hexyl n-caproate, n-caproic acid (E) -2-hexenyl), and those components are converted into glass capillaries, gray rubber septum, and white rubber septum. The attractiveness of the loaded attractive preparation was clarified (published on the homepage at http://www.agri.pref.hokkaido.jp).

  Furthermore, elucidation of attractiveness and optimum mixing ratio (100: 40: 3) of sex pheromone three components (n-hexyl n-caproate: n-caproic acid (E) -2-hexenyl: n-octyl n-butyrate) The optimum loading of sex pheromone in the glass capillary preparation (4.29-14.3 μg / formulation) was reported (Reference 1: Kakizaki and Sugie (2001) J. Chem. Ecol. 27: 2447-2458.) .

When actually monitoring, in order to stabilize the amount of sex pheromone released over a long period of time, conventionally, for example, glass capillaries and rubber septa were used. As an attractive preparation using a rubber septum, there is Patent Document 1.
JP 2005-47889 A

  There are two conventional problems.

  First, attractant preparations using only the known three components have unstable attractability and variation in the number of captured insects, which is insufficient to realize a highly accurate and stable survey, and further enhance attractability. It was necessary to study the addition of ingredients.

  Secondly, there is a problem of technology for stably releasing sex pheromones over a long period of time. Glass tubules in which sex pheromones are encapsulated exhibit an attractiveness superior to that of rubber septum preparations. However, glass capillaries are easily broken and have a drawback that requires careful handling.

  In addition, rubber septums that are widely used as a material for attractant preparations of sex pheromones such as moss have the disadvantage that the release amount of sex pheromones is large at the initial stage of installation and thereafter the release amount decreases rapidly. This is more conspicuous as the sex pheromone substance has a smaller molecular weight and higher volatility. For this reason, the attractiveness changes greatly with time and is not constant. (Reference: Sex pheromone experiment method (2) Japan Plant Protection Association (1984), Hirano, Horiike, preparation adjustment method p.4-23). The stink bug sex pheromone substance has 8-12 carbon atoms, and its molecular weight is considerably smaller than that of the moss sex pheromone substance (14-22 carbon atoms), and its volatility is high. For this reason, when rubber septums are used in the preparation, the release rate of the sex pheromone from the attractive preparation changes greatly with time, so it is difficult to keep the attractiveness to the stink bug constant. In this way, in the attractive formulation that only supports the known sex pheromone ternary component with rubber septum, stable attractiveness is not sustained, and it is difficult to grasp the exact occurrence and development of this stink bug. It is a situation where the purpose cannot be achieved for use in a practical situation where a high-precision survey is required, such as a judgment of the above.

  Therefore, the object of the present invention is to improve the components in order to exhibit a more stable and reliable attracting ability, and secondly, to release the improved pheromone stably into the air for a long period of time. It is in the point to make it possible.

  In order to achieve the above-mentioned object, the sex pheromone sustained-release attractant formulation of red-bellied squirrel mosquito according to claim 1 is known as an attractive sex pheromone n-caproate n-hexyl, n-caproic acid (E) N-Hexyl acetate is added as a fourth component to 2-hexenyl and n-octyl n-butyrate. Thereby, it was confirmed that attraction performance is improved.

  In claim 2, n-hexyl acetate as the fourth component is added in an amount of 0.1 to 0.3% of the first component (n-hexyl n-caproate). When this amount is added, particularly attractive attracting performance is obtained.

  Claim 3 relates to a resin capable of stably releasing long-term sex pheromone into the air, and is composed of four component sex pheromones (n-hexyl n-caproate, n-caproic acid (E) -2-hexenyl). When n-octyl butyrate, n-hexyl acetate) is uniformly kneaded with a sustained-release resin (for example, paraffin), the sex pheromone is gradually released into the air according to volatilization from the resin.

  However, even in this case, the amount of sex pheromone released varies depending on various conditions, for example, the number of days elapsed after installation, temperature, and humidity.

  According to a fourth aspect of the present invention, a sex pheromone mixed with a resin is placed in a container having an opening. According to this, the release amount of the sex pheromone can be adjusted by the release speed from the sustained-release resin and the opening diameter of the container, and a stable result can be obtained over a long period of time.

  The fifth aspect relates to the material of the container. The container is formed of a material having a sustained release property that transmits sex pheromone (n-hexyl n-caproate, n-caproic acid (E) -2-hexenyl, n-octyl n-butyrate, n-hexyl acetate). . If the material of the container is a material that transmits sex pheromone, the diffusion rate of the sex pheromone is controlled by the transmittance of the container material, and even if the sex pheromone is sealed without opening, the attracting performance of the sex pheromone is achieved. It can be demonstrated.

  The sixth aspect relates to the material of the container for containing the sex pheromone mixed with the resin. The container is made of a material with low permeability to sex pheromones (n-hexyl n-caproate, n-caproic acid (E) -2-hexenyl, n-octyl n-butyrate, n-hexyl acetate) (a material that hardly permeates). By molding. In the case of a container having an opening, it is easy to control the sustained release according to the opening diameter, and it is possible to ensure a long-term stable attracting performance.

  According to the attraction preparations according to claims 1 and 2, it becomes possible to exert a higher attraction ability than the conventional attraction preparation. According to the attraction preparations according to claims 3 to 6, a stable amount of sex pheromone can be obtained over a long period of time. Can be released into the air.

  The present inventor conducts research on the aforementioned problems related to the stink bug sex pheromone, and searches for attracting ingredients and examines preparation methods in the field attraction test using the extracted components and chemical synthetic products of the stink bug female adults. Thus, a novel attractant component was discovered, and a sustained-release preparation was found for a long period of time. The present invention has been completed based on these findings.

  First, the outline of the attractive preparation according to the present invention will be described.

  The component of the sex pheromone used as the attraction preparation is a known sex pheromone three component (first component n-caproic acid n-hexyl: second component n-caproic acid (E) -2-hexenyl: third component n-butyric acid n It was confirmed that the attractiveness was improved by adding a novel fourth component (n-hexyl acetate) to -octyl = 100: 40: 3 (an example of a mixing ratio)).

  When the fourth component is added at a high concentration, the fourth component may exhibit an attraction inhibitory action, and therefore the fourth component is 0.1 to 0.00 with respect to the amount of the first component (n-hexyl caproate). It is preferable to add in the range of 3%. As a result, the attractiveness is improved by 1.2 to 2.3 times compared to the case of using only the known three components, the variation in the number of captures is reduced, the attractiveness is stabilized, and an excellent effect in terms of improving the attractiveness is exhibited. . Specific experimental results are described in the examples below.

  Next, in order to obtain effects by releasing these four-component sex pheromones stably in the air over a long period of time (reference: Atterholt et al. (1999) J. Controlled Release 57 : Sex pheromone consisting of four components is mixed with resin such as paraffin (paraffin wax) reported as 233-247.).

  The amount of the sex pheromone added to the paraffin resin is equivalent to 1 to 10000 μg / g resin (0.001 to 10 mg / g resin), preferably 30 based on the amount of the first component (n-hexyl n-caproate). -1000 μg / resin g (0.03 to 1.0 mg / resin g) in an equivalent amount range, more preferably 100 μg / resin g (0.1 mg / resin g) in an equivalent amount. Harden and mold.

  The amount of paraffin resin used as one preparation (attractive preparation) is in the range of 0.1 to 100 g, preferably 0.5 to 3 g. It is desirable to do.

  Next, a description will be given of the best mode for specifically performing monitoring using a sex pheromone obtained by adding a new component to three known components.

  A fourth component (n-hexyl acetate) is added to three known sex pheromone components (n-hexyl: n-caproate: n-caproic acid (E) -2-hexenyl: n-octyl n-butyrate) of the red beetle moss turtle. A mixture of sex pheromone added in an amount of 0.1 to 0.3% is 30 to 1000 μg / g resin (0.03 to 1.0 mg / g resin) based on the amount of the first component (n-hexyl n-caproate). ) In a ratio of 0.3 to 1 g, which is solidified by adding and mixing to a paraffin resin (melting point: 50 to 52 ° C.) A glass bottle or a metal container or the like; an attractant preparation that is placed in an open mouth area of about 0.3 to 0.8 cm 2 and whose release amount is controlled by the size of the open mouth is the most preferred embodiment.

  The content and ratio of each active ingredient of the sex pheromone added to the resin can be appropriately determined according to various conditions such as the installation environment.

  Solid paraffin is used as the sustained-release material of the sex pheromone, but those having various melting points can be used depending on the situation. In addition to solid paraffin, liquid paraffin, waxes, and various sustained-release resins can be used as sustained-release materials.

  Resins to which sex pheromones are added can be used as they are. This is because the attractiveness is higher than the conventional one and the sustained release property is also excellent. However, preferably, a container using a material that allows the sex pheromone component to permeate to some extent, such as polyethylene, or a material that is difficult to permeate the sex pheromone component, in order to ensure sustained release more highly. It is used by being loaded into a container using, for example, a container made of polypropylene, polystyrene, fluororesin, glass, metal, etc., having an open aperture that can easily maintain sustained release. The shape of the container is arbitrary. Existing product containers can also be used.

  In using the attractant preparation, an appropriate amount of an ultraviolet absorber, for example, an antioxidant such as butylhydroxytoluene or vitamin E, 2-hydroxy-4-octbenzophenone, or the like may be added to paraffin or the container material.

Example 1
Below, the Example regarding the addition amount of a novel component (4th component) is illustrated.
The test inducement preparation is a known sex pheromone three component (first component: second component: third component = 100: 40: 3), and the fourth component is in the range of 0.03 to 10% of the first component amount. In a solid paraffin resin (melting point: 50-52 ° C., the same applies to other examples) dissolved in 60-70 ° C., the amount of the first component is 100 μg / resin g (0.1 mg / resin g). A solution obtained by adding 0.8 g of a suitable amount added and mixed into a polyethylene container and hardening it was used.

  A polyethylene container having a length of 18.9 mm, a diameter of 8.1 mm, and a wall thickness of 0.75 mm was used. As this polyethylene container, a commercially available product can be used.

  This was installed on the ground of an Italian ryegrass grass field at intervals of about 10 m, and the number of captures in each trap was investigated every day to every few days. The total number of captures during the survey period was summed to calculate the average attracted argument. This is shown in Table 1.

  From this test result, it can be seen that by adding 0.1 to 0.3% of the fourth component with respect to the first component, the number of attracting arguments is increased compared to the known three-component attractant formulation. In this test example, when the addition ratio of the fourth component exceeded 10%, the number of attracting arguments was smaller than that of the known three components alone, and the effect of suppressing attracting was observed at a high concentration. The material is different.

Example 2.
Next, as an examination of the amount of synthetic pheromone added to the solid paraffin resin, 10 to 1000 μg / resin g (0 g) of the synthetic pheromone three components (the above-mentioned mixing ratio) with respect to 1 g of solid paraffin as the amount of the first component. .01-1.0 mg / resin g), and a preparation containing 0.8 g of the resin in a polyethylene container (described above) was prepared, and the attractiveness in the field was compared in the same manner as in Example 1. did.

  The amount of the synthetic pheromone added per 1 g of the solid paraffin resin is high in the range of 30 μg to 1000 μg / resin g (0.03 to 1.0 mg / resin g) in terms of the first component, particularly 100 μg / resin g ( 0.1 mg / resin g) was the most attractive.

Example 3.
In order to confirm the amount of sex pheromone released from the formulation, the first component amount of 100 μg / resin g (0.1 mg / resin g) of a mixture of the three components of the synthetic pheromone (the aforementioned mixing ratio) per 1 g of solid paraffin resin 0.8 g of the resin added with the product directly molded (short cylindrical shape with a diameter of 8 mm and a length of 16 mm), and the resin made of polypropylene (length 38.5 mm, diameter 8.9 mm (open mouth area approximately 62 mm2) ), Wall thickness of 0.75 mm, pitch-type tube, no lid: This material was made into a sex pheromone of this stink bug, which is impermeable. These preparations were placed in a fume hood under constant temperature conditions (22 ° C.), and sex pheromones released from each preparation over 80 days after installation were adsorbed on Polar Pack Q and analyzed with a gas chromatography apparatus. Hourly release amounts (ng / hr) of one component were compared.

  The amount of the synthetic pheromone released showed a gradual decrease in 80 days from the installation day in the solid paraffin resin-only preparation (no container). On the other hand, in the preparation in which this resin was put in a polypropylene container, a substantially constant release amount was maintained over 60 days. This is shown in FIG.

Example 4.
In order to confirm the duration of attractiveness in the field, synthetic pheromone four components (first component: second component: third component: fourth component = 100: 40: 3: 0.1) are used as the first component A trap with an attractant formulation containing 0.8 g of solid paraffin resin added with an equivalent amount of 100 μg / resin g (0.1 mg / resin g) in a polypropylene container (described above) in three periods (late May, 7 In the middle of the month and late August, it was installed in the field (the paddy shore) and compared the sustainability of the attraction.

  The area of late-May and mid-July installation of this attractant product has the same length of attraction to the end of August until mid-October, and this product has maintained a certain level of attractiveness for at least 3 months. It was confirmed. Also, in this example, it was confirmed that even if the preparation was replaced in the middle, the attracting argument would hardly fluctuate between the new and old preparations, and this attracting preparation remained stable as a monitoring preparation for a long period of time. I confirmed that This is shown in FIG.

  In addition, as a result of comparison between the length of attraction by sex pheromone trap with attractant preparation in this survey and the length of capture by the scooping method, which is a conventional survey method, the pheromone trap is about 5 times the capture by scooping method. It was confirmed that the survey method was simple and highly accurate with a large number of insects and clear fate.

  In addition, the state which packed the solid paraffin resin 2 in the polypropylene container 1 and solidified is illustrated in FIG. The solid paraffin resin 2 carries a sex pheromone composed of four components. Reference numeral 3 denotes an opening for releasing a sex pheromone. The lid may or may not be present. This is because the lid is opened during monitoring.

  FIG. 4 illustrates a net cylindrical trap 5 in which the preparation (1) is installed. The net cylinder trap 5 is a 5 mm-thick black polyethylene net having a cylindrical shape with a length of 30 cm and a diameter of 6 cm. After standing this vertically on the ground and spraying an adhesive spray on the net, the formulation (1) was suspended inside the net cylindrical trap 5. 6 is a string. The opening 3 is opened downward. Install this in the field.

  The attractant preparation according to the present invention can be used by attaching to various traps in addition to such a net cylindrical trap. Depending on the purpose of the survey, an appropriate number of traps should be installed on the fields such as paddy fields and the surrounding shores that are the source of this species, and on grasses and weedlands. The traps are slightly above the surface of the crop. Install.

It is a graph which shows the discharge amount transition of the sex pheromone which concerns on an Example. It is the graph which confirmed the duration of the attractiveness concerning an example. It is a figure which illustrates the container which concerns on an Example. It is a figure which illustrates the net cylindrical trap which concerns on an Example.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Polypropylene container 2 Solid paraffin resin 3 Opening 5 Net cylindrical trap 6 String

Claims (6)

  N-hexyl acetate, n-hexyl acetate, n-hexyl n-caproate, (E) -2-hexenyl n-caproate, n-octyl acetate, which is a known synthetic pheromone of the red beetle moss turtle, and n-hexyl acetate as the fourth component A sex pheromone sustained-release attractant preparation for red-bellied flies.   The fourth component, n-hexyl acetate, is added with 0.1 to 0.3% of n-hexyl n-caproate, which is a known synthetic pheromone. Sexual pheromone sustained-release attractant preparation for Midorikasikameka.   It is characterized by mixing n-hexyl n-caproate, n-caproic acid (E) -2-hexenyl, n-octyl n-butyrate, and n-hexyl acetate with a sustained-release resin and charging it into a container. The sex pheromone sustained-release attractant preparation of the red-bellied fosmidridika turtle according to claim 1 or 2.   The container according to claim 3 is provided with an opening, and the sex pheromone sustained-release attractant preparation for red-bellied flies.   The container according to claim 3 or 4, wherein the components of n-caproic acid n-hexyl, n-caproic acid (E) -2-hexenyl, n-butyric acid n-octyl, and n-hexyl acetate are permeated slowly. 5. A sex pheromone sustained-release attractant preparation for red-bellied flies, which is molded from a material having properties.   The container according to claim 3 or 4 is made of a material that does not allow components of n-caproic acid n-hexyl, n-caproic acid (E) -2-hexenyl, n-octyl butyrate, and n-hexyl acetate to permeate. 5. A sex pheromone sustained-release attractant preparation for red-bellied flea midrib turtles according to claim 3 or 4, which is molded.

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