JP2008245942A - Blood component collecting apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a blood component collecting apparatus suppressing reduction in the collection ratio of a blood component to be collected, while preventing the reduction of the amount of treatment blood. <P>SOLUTION: The blood component collecting apparatus is configured to perform a plasma circulation process by operating a second solution sending pump without interrupting a plasma collecting process in the middle of the plasma collecting process being performed by operating a first solution sending pump. When the amount of the treatment blood becomes a prescribed amount in the middle of the performance of the plasma circulation process, the solution sending speed of the second solution sending pump is reduced so as to reduce a plasma circulation flow rate. That is, the solution sending speed of the second solution sending pump is comparatively highly set so as to increase the plasma circulation flow rate in a period from the start of the plasma circulation process till the amount of the treatment blood becomes the prescribed amount. Subsequently after the amount of the treatment blood becomes the prescribed amount, the solution sending speed of the second solution sending pump is comparatively lowly set, so as to reduce the plasma circulation flow rate. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a blood component collection device.

  When collecting blood, the collected blood is separated into each blood component by centrifugation, etc. for reasons such as effective use of the blood and reduction of the burden on the donor, and only the components necessary for the transfuser are collected. Blood is collected to return to the person.

  In such component blood collection, blood collected from a blood donor is introduced into a blood component collection circuit using a blood component collection device, and plasma is collected by a centrifuge called a centrifuge bowl installed in the blood component collection circuit. The buffy coat and red blood cells are separated, the platelets (platelet containing plasma) are separated from the buffy coat, the platelets containing plasma are collected in a platelet collection bag to obtain a platelet preparation, and the plasma is also collected in the plasma collection bag. The remaining plasma, white blood cells, and red blood cells are used as raw materials for the plasma preparation or plasma fraction preparation, and returned to the donor (see, for example, Patent Document 1).

  In this blood component collection device, a plasma collection step of collecting plasma in a plasma collection bag, a plasma circulation step of circulating the plasma collected in the plasma collection bag so as to pass through a centrifuge, and collecting platelets in the platelet collection bag A blood component collecting operation including a platelet collecting step and a blood returning step for returning the remaining blood components to the blood donor is performed for a plurality of cycles. The plasma collection step and the plasma circulation step are performed with time overlap.

  By performing the plasma circulation step, platelets trapped by the red blood cell layer and the like can be washed out in the centrifuge, and the concentration of the blood cell layer (red blood cell layer and buffy coat layer) is suppressed, thereby When separating the platelets, the platelets can be collected in a state where the platelet particles are well dispersed in the plasma, so that the recovery rate of the platelets can be improved and the variation in the recovery rate can be suppressed.

  This plasma circulation process cannot be performed continuously unless a sufficient amount of plasma is collected in the plasma collection bag.

  That is, in the first cycle, the plasma circulation process cannot be started until an amount of plasma that can execute the plasma circulation process is collected in the plasma collection bag. For this reason, the blood cell layer is concentrated in the centrifuge until the plasma circulation step is started, and the recovery rate of platelets is lowered.

  In each cycle after the second cycle, the plasma collection step and the plasma circulation step can be started almost simultaneously, but before the plasma separated from the blood collected from the donor begins to be introduced into the plasma collection bag. Since it takes a predetermined time, the amount of plasma in the plasma collection bag is reduced by the amount that the plasma in the plasma collection bag is transferred into the centrifuge during the plasma circulation process. The amount of plasma in the blood may be less than the amount that can perform the plasma circulation process. In particular, blood donors with high hematocrit values have a high probability because the amount of plasma collected in one cycle is small. For this reason, the plasma circulation process must be interrupted, and the plasma circulation process cannot be resumed until an amount of plasma capable of performing the plasma circulation process is collected in the plasma collection bag. Due to the interruption of the plasma circulation process, the blood cell layer is concentrated in the centrifuge, and the platelet recovery rate is lowered.

  By the way, in the conventional blood component collection device, in the plasma circulation process that is executed in an overlapping manner with the plasma collection process, the plasma is circulated at a high speed so that the flow rate of the circulating plasma is increased. The effect of the circulation can be improved, so that the plasma circulation step performed alone can be omitted, but since the flow rate of the circulating plasma is increased, the amount of blood to be processed is reduced accordingly, The collection rate of collected platelets is reduced. For this reason, after the plasma collection step is completed, the plasma is circulated at a low speed, the flow rate of the circulating plasma is reduced, and the plasma circulation step is continued, and the additional plasma collection step is performed.

  However, in the conventional blood component collection device, in each cycle after the second cycle, the plasma separated from the blood collected from the blood donor starts before being introduced into the plasma collection bag before the plasma collection bag. Since the plasma in the plasma collection bag is transferred at high speed into the centrifuge, the amount of plasma in the plasma collection bag is greatly reduced. On the way, the amount of plasma in the plasma collection bag may be less than the amount that can perform the plasma circulation process, and the plasma circulation process may be interrupted. That is, in the conventional blood component collection device, it is not assumed that the high-speed plasma circulation process is interrupted. As a result, the blood cell layer is concentrated in the centrifuge, and the platelet recovery rate decreases.

JP 2004-105582 A

  An object of the present invention is to provide a blood component collection device capable of stably collecting blood cell components while suppressing a decrease in the recovery rate of blood cell components to be collected while preventing the amount of treated blood from decreasing. It is in.

Such an object is achieved by the present inventions (1) to (14) below.
(1) a blood collection means provided with a hollow needle for collecting blood from a blood donor;
A blood separator for separating blood collected by the blood collecting means;
A plasma collection bag for collecting plasma separated by the blood separator;
A blood component collection bag for collecting predetermined blood cell components separated by the blood separator;
A blood treatment line connecting the hollow needle and the inlet of the blood separator;
A blood component collection circuit comprising a plasma circulation line branched from a branch portion provided in the blood treatment line and connected to the plasma collection bag;
A first liquid delivery means installed in the blood treatment line and delivering at least a liquid in the blood treatment line;
A second liquid feeding means installed in the plasma circulation line and for feeding at least the plasma in the plasma collection bag;
A plasma collecting step of operating the first liquid feeding means, transferring the blood collected by the blood collecting means to the blood separator, and collecting the plasma separated by the blood separator in the plasma collection bag; The second liquid feeding means is operated to transfer the plasma collected in the plasma collection bag to the blood separator via the plasma circulation line, and between the plasma collection bag and the blood separator. The first blood circulation step for circulating the blood plasma and the blood component collection step for collecting the predetermined blood cell component in the blood component collection bag by operating the second liquid feeding means. Liquid feeding means and liquid feeding control means for controlling the operation of the second liquid feeding means,
The liquid supply control means operates the second liquid supply means without interrupting the plasma collection step while the plasma collection step is being performed by operating the first liquid supply unit. The blood circulation process is performed, and after the plasma collection process is completed, the blood component collection process is performed, and the processed blood volume reaches a predetermined amount during the plasma circulation process. The blood component collection is characterized by having a flow rate control function for changing the flow rate of plasma circulating between the plasma collection bag and the blood separator by changing the liquid supply speed of the second liquid supply means. apparatus.

  (2) The flow rate control function circulates between the plasma collection bag and the blood separator by reducing the liquid feeding speed of the second liquid feeding means when the processed blood volume reaches a predetermined volume. The blood component collection device according to (1), which is configured to reduce the flow rate of plasma.

  (3) The liquid feeding control means performs a blood component collection operation including the plasma collection step, the plasma circulation step, the blood component collection step, and a blood return step of returning the remaining blood components to the blood donor. The blood component collecting apparatus according to (1) or (2), wherein the blood component collecting apparatus is executed at least for one cycle.

  (4) In the first cycle, the flow rate control function starts the plasma circulation step when the amount of plasma collected in the plasma collection bag reaches a first amount capable of executing the plasma circulation step. The blood component collection device according to (3), which is configured as described above.

  (5) The liquid feeding control means starts the plasma collection step and the plasma circulation step substantially simultaneously in each cycle after the second cycle, and is executing the plasma circulation step while the plasma circulation step is being executed. When the amount of plasma collected in the collection bag becomes less than a first amount capable of performing the plasma circulation step, the plasma circulation step is interrupted, and the amount of plasma collected in the plasma collection bag is changed to the first amount. When the amount reaches, the plasma circulation process is resumed, the liquid delivery speed of the second liquid delivery means before the plasma circulation process is interrupted, and the second liquid delivery after the plasma circulation process is resumed. The blood component collecting device according to (3) or (4), wherein the blood component collecting device has a flow rate control function for setting the liquid feeding speed of the means to different values.

  (6) The flow rate control function according to (5), wherein the flow rate control function is configured to increase a liquid feeding speed of the second liquid feeding unit after the plasma circulation process is resumed than before the interruption. Blood component collection device.

(7) Whether the amount of plasma collected in the plasma collection bag during execution of the plasma circulation step is less than a first amount at which the plasma circulation step can be performed is determined. A prediction means for predicting based on the value;
The liquid feeding control unit starts the plasma collection step and the plasma circulation step substantially simultaneously in each cycle after the second cycle, and the plasma is being performed while the plasma circulation step is being executed by the prediction unit. If the amount of plasma collected in the collection bag is predicted to be less than the first amount, the amount of plasma collected in the plasma collection bag during the execution of the plasma circulation step Is less than or equal to a second amount greater than the first amount, the liquid feeding speed of the second liquid feeding means is increased, and then the amount of plasma collected in the plasma collection bag becomes the first amount. (3) having a flow rate control function of interrupting the plasma circulation step when the amount is less than the amount and resuming the plasma circulation step when the amount of plasma collected in the plasma collection bag reaches the first amount Or blood according to (4) Minute sampling device.

  (8) The flow rate control function is characterized in that the second liquid feeding means after the plasma circulation process is resumed from the liquid feeding speed of the second liquid feeding means before the plasma circulation process is interrupted. The blood component collection device according to (7), which is configured to reduce the speed.

  (9) The flow rate control function circulates between the plasma collection bag and the blood separator by decreasing the liquid feeding speed of the second liquid feeding means when the processed blood volume reaches a predetermined volume. The blood component collection device according to (7), wherein the blood component collection device is configured to reduce a flow rate of plasma.

  (10) The flow rate control function includes: a liquid feeding speed of the second liquid feeding unit before the plasma circulation step is interrupted; and a liquid feeding of the second liquid feeding unit after the plasma circulation step is resumed. The blood component collection device according to (9), which is configured to have substantially the same speed.

(11) a blood collecting means comprising a hollow needle for collecting blood from a blood donor;
A blood separator for separating blood collected by the blood collecting means;
A plasma collection bag for collecting plasma separated by the blood separator;
A blood component collection bag for collecting predetermined blood cell components separated by the blood separator;
A blood treatment line connecting the hollow needle and the inlet of the blood separator;
A blood component collection circuit comprising a plasma circulation line branched from a branch portion provided in the blood treatment line and connected to the plasma collection bag;
A first liquid delivery means installed in the blood treatment line and delivering at least a liquid in the blood treatment line;
A second liquid feeding means installed in the plasma circulation line and for feeding at least the plasma in the plasma collection bag;
A plasma collecting step of operating the first liquid feeding means, transferring the blood collected by the blood collecting means to the blood separator, and collecting the plasma separated by the blood separator in the plasma collection bag; The second liquid feeding means is operated to transfer the plasma collected in the plasma collection bag to the blood separator via the plasma circulation line, and between the plasma collection bag and the blood separator. The first blood circulation step for circulating the blood plasma and the blood component collection step for collecting the predetermined blood cell component in the blood component collection bag by operating the second liquid feeding means. Liquid feeding means and liquid feeding control means for controlling the operation of the second liquid feeding means,
The liquid feeding control means uses the liquid feeding amount of the first liquid feeding means as a reference when the first liquid feeding means and the second liquid feeding means are operating in time overlap. The blood has a flow rate control function for changing the flow rate of the plasma circulating between the plasma collection bag and the blood separator by changing the liquid feeding speed of the second liquid feeding means. Component collection device.

  (12) The flow rate control function operates the second liquid feeding unit without interrupting the plasma collecting step while the plasma collecting step is being performed by operating the first liquid feeding unit. When the plasma circulation process is completed, the blood collection process is performed, and the blood component collection process is performed, and the plasma collection process is performed while the plasma circulation process is being performed. When the amount of plasma is less than a predetermined amount, the plasma circulation step is interrupted, and when the amount of plasma collected in the plasma collection bag reaches the predetermined amount, the plasma circulation step is resumed, and the plasma circulation step The liquid feeding speed of the second liquid feeding means before interruption of the plasma and the liquid feeding speed of the second liquid feeding means after resuming the plasma circulation step are set to different values. The blood component collection device according to (11) above

(13) Whether or not the amount of plasma collected in the plasma collection bag during execution of the plasma circulation step is less than a first amount at which the plasma circulation step can be performed is determined. A prediction means for predicting based on the value;
The flow rate control function operates the second liquid delivery means without interrupting the plasma collection process while the plasma collection process is being performed by operating the first liquid delivery means. After the plasma circulation step is executed and the plasma collection step is completed, the blood component collection step is executed, and the plasma collecting step is collected in the plasma collection bag while the plasma circulation step is being executed by the prediction means. When the amount of plasma collected is predicted to be less than the first amount, the amount of plasma collected in the plasma collection bag during the execution of the plasma circulation step is less than the first amount. When the amount is less than or equal to a second amount greater than the amount, the liquid feeding speed of the second liquid feeding means is increased, and then the amount of plasma collected in the plasma collection bag is less than the first amount, The plasma circulation process is interrupted and collected in the plasma collection bag. If the amount of plasma which is to reach the first amount, the blood component collection apparatus according to resume the (11) the plasma circulation step.

  (14) The blood component collection device according to any one of (1) to (13), wherein the predetermined blood cell component is platelets.

  ADVANTAGE OF THE INVENTION According to this invention, the fall of the collection | recovery rate of the blood cell component (for example, platelets) extract | collected can be suppressed, preventing the processing blood volume decreasing.

  In particular, when the amount of processed blood reaches a predetermined amount, it is configured to change the flow rate of plasma circulating between the plasma collection bag and the blood separator, so that the plasma circulation process in the first cycle is started. The influence of interrupting the plasma circulation during the period until it becomes possible or after the second cycle can be reduced. Thereby, a blood cell component can be collected efficiently and stably.

  In addition, blood collection time can be shortened. Thereby, the occupation time of the blood component collection device can be reduced, and the burden on the blood donor can be reduced.

  Hereinafter, the blood component collection device of the present invention will be described in detail based on preferred embodiments shown in the accompanying drawings.

<First Embodiment>
FIG. 1 is a plan view showing a first embodiment of a blood component collection device of the present invention. FIG. 2 is a diagram showing a centrifugal separator mounted on the centrifuge drive device provided in the blood component collection device shown in FIG. It is a partially broken sectional view of a state.

  A blood component collection apparatus 1 shown in FIG. 1 separates blood into a plurality of blood components, and separates blood platelets (platelets including plasma) (blood components), which are predetermined blood cell components, and plasma (blood components). It is a device for collecting. The blood component collection device 1 has a rotor 142 having a blood storage space 146 therein, an inlet 143 communicating with the blood storage space 146, and an outlet (outlet) 144. The rotor 142 rotates to rotate the inlet 142 from the inlet 143. A centrifuge (blood separator) 20 that centrifuges the introduced blood in the blood storage space 146, a first line 21 that connects a blood collection needle (blood collection means) 29 and the inlet 143 of the centrifuge 20; The second line 22 connected to the outlet 144 of the centrifuge 20, the third line 23 connected to the first line 21, and the first line 21 via the tubes 49 and 50. And a plasma collection bag (collection bag) 25 connected to the second line 22 via the tubes 43 and 44, and an air connected to the second line 22 via the tube 42. The bag 27b, an intermediate bag (temporary storage bag) (collection bag) 27a connected to the second line 22 via the tubes 43 and 45, and an intermediate bag 27a connected via the tubes 46, 47 and 48 A blood component collection circuit (collection circuit) 2 having a platelet collection bag (collection bag) 26 and a bag 28 connected to the platelet collection bag 26 via a tube 51 is provided.

  Further, the blood component collecting device 1 includes a centrifuge driving device 10 for rotating the rotor 142 of the centrifuge 20, and a first liquid feeding pump (first liquid feeding means) for the first line 21. ) 11, a second liquid feed pump (second liquid feed means) 12, a third liquid feed pump (third liquid feed means) 13 for the third line 23, and a blood component collection circuit A plurality of (sixth, first to sixth in this embodiment) channel opening / closing means 81, 82, 83, 84, 85, 86 that can open and close the middle of the two channels, and the centrifuge drive device 10. A control unit (control means) 3 for controlling the first liquid delivery pump 11, the second liquid delivery pump 12, the third liquid delivery pump 13, and the plurality of flow path opening / closing means 81 to 86, and turbidity Sensor (platelet concentration sensor) 14, optical sensor 15, weight sensor 16, and a plurality (this embodiment) It is provided with a bubble sensor 31,32,33,34,35,36 of six).

First, the blood component collection circuit 2 will be described.
This blood component collection circuit 2 connects a blood collection needle (hollow needle) (blood collection means) 29 for collecting blood from a donor (blood donor) and an inlet 143 of the centrifuge 20 and connects the first pump tube 21g. A first line 21 (blood collection and blood return line) (blood treatment line) 21 that is also used as both a blood collection line and a blood return line, and one end side is connected to an outlet (outlet) 144 of the centrifuge 20 The second line 22 formed, the third line (anticoagulant injection line) 23 connected to the blood collection needle 29 of the first line 21 and including the third pump tube 23a, and the first line 21 is connected to the centrifuge 20 side from the first pump tube 21g, a tube 49 connected to the tube 50, a part of which constitutes the second pump tube 22a, and a second line. 22, a tube 44 connected to the tube 43, a plasma collection bag 25 connected to the tubes 44 and 49, a tube 42 connected to the second line 22, and a connection to the tube 42 Air bag 27b, tube 45 connected to tube 43, intermediate bag 27a connected to tube 45, tube 46 connected to intermediate bag 27a, tube 47 connected to tube 46, tube 48, a platelet collection bag 26 connected to the tube 48, a tube 51 connected to the platelet collection bag 26, and a bag 28 connected to the tube 51. The air bag 27b and the intermediate bag 27a are integrally formed (integrated).

  The first line 21 is connected to the blood collection needle side first line 21a to which the blood collection needle 29 is connected, one end side is connected to the blood collection needle side first line 21a, and the other end side is connected to the inlet 143 of the centrifuge 20. Centrifuge side first line 21b. As the blood collection needle 29, for example, a known metal needle is used.

  The blood collection needle side first line 21a, the centrifuge side first line 21b, the second line 22 and the third line 23 described later are each connected by a soft resin tube or a plurality of soft resin tubes. Has been formed.

  The blood collection needle side first line 21a is connected to the third line 23 from the blood collection needle 29 side, the chamber 21d for removing bubbles and microaggregates, and the branch connector for connection to the tube 50. And a first pump tube 21g formed between the chamber 21d and the branch connector 21f.

  Air bubble sensors 35, 36 and 32 are installed along the blood collection needle side first line 21a from the blood collection needle 29 side. In this case, the bubble sensors 35 and 36 are disposed between the branch connector 21c and the chamber 21d, and the bubble sensor 32 is disposed between the chamber 21d and the first pump tube 21g.

  The bubble sensors 35, 36, and 32 transmit and receive ultrasonic waves from the outside of the tube, and make use of the fact that the ultrasonic conductivity differs between the liquid and the bubbles (gas), thereby using the gas and liquid (gas / liquid) in the tube. Or a gas / liquid level). The bubble sensors 31, 33 and 34 are also detection means having the same function as described above. Further, the bubble sensor (gas and liquid detection means) is not limited to the ultrasonic sensor, and for example, an optical sensor, an infrared sensor, or the like may be used.

  The chamber 21d is connected with a gas-permeable and bacteria-impermeable filter 21i through a tube 21h. This line can be used for detecting the internal pressure of the blood collection needle side first line 21a, for example.

  On the other hand, the centrifuge side first line 21 b is connected to a branch connector 21 f for connection with the tube 50.

  One end of the second line 22 is connected to the outlet 144 of the centrifuge 20.

  The second line 22 includes a branch connector 22b for connection to the tubes 42 and 43.

  A turbidity sensor 14 and a bubble sensor 34 are installed along the second line 22 from the centrifuge 20 side. In this case, the turbidity sensor 14 and the bubble sensor 34 are disposed between the centrifuge 20 and the branch connector 22b.

  The branch connector 22b is connected with a filter 22f that is air-permeable and bacteria-impermeable through a tube 41. This line can be used for detecting the internal pressure of the second line 22, for example.

  One end of the third line 23 is connected to a connecting branch connector 21 c provided on the first line 21. That is, the third line (flow path) 23 branches from the first line (flow path) 21 via the branch connector (branch portion) 21c. The branch connector 21c is located (provided) in the vicinity of the blood collection needle 29.

  The third line 23 includes, from the branch connector 21c side, a third pump tube 23a, a sterilizing filter (foreign matter removing filter) 23b, a bubble removing chamber 23c, and an anticoagulant container connecting needle 23d. It has.

  A bubble sensor 31 is installed along the third line 23. The bubble sensor 31 is disposed between the branch connector 21c and the third pump tube 23a.

  The anticoagulant container connecting needle 23d of the third line 23 is connected to a container (not shown) in which an anticoagulant (anticoagulant liquid) is housed (contained), whereby the anticoagulant in the container is As will be described later, the anticoagulant container connecting needle 23d flows through the third line 23 toward the branch connector 21c and is supplied (injected) to the blood collection needle side first line 21a. Thereby, for example, the anticoagulant can be added (mixed) to the blood collected by the blood collection needle 29 via the third line 23.

  In addition, although it does not specifically limit as an anticoagulant, For example, ACD-A liquid etc. can be used.

  The plasma collection bag 25, which is a blood component collection bag, is a container for collecting (storing) plasma (plasma component) (second blood component). One end of the tube 49 is connected to the plasma collection bag 25, and a connecting branch connector 22d is provided in the middle thereof. One end of the tube 50 is connected to the branch connector 22d, and the other end is connected to the branch connector 21f. The second pump tube 22a is located between the plasma collection bag 25 and the branch connector 22d. The tubes 49 and 50 and the branch connector 22d constitute the main part of the plasma circulation line 24.

  One end of the tube 43 is connected to the branch connector 22b, and the other end is provided with a connection branch connector 22c. One end of the tube 44 is connected to the branch connector 22c, and the other end is connected to the plasma collection bag 25.

  A bubble sensor 33 is installed along the tube 46 in the middle of the tube 46.

  The plasma collection bag 25 and tubes 43 and 44 constitute a plasma collection branch line for collecting plasma.

  A platelet (platelet preparation) collection bag 26, which is a blood component collection bag, collects (stores) platelets (platelet component) (blood cell component) (first blood component) containing plasma after passing through a leukocyte removal filter 261 described later. ). In the following description, platelets including plasma (first blood component) are referred to as “concentrated platelets”, and concentrated platelets collected (stored) in the platelet collection bag 26 are referred to as “platelet preparations”.

  One end of the tube 51 is connected to the platelet collection bag 26, and the bag 28 is connected to the other end.

  The air bag 27b is a container for temporarily storing (reserving) air.

  At the time of blood collection to be described later, air (sterilized air) in the blood component collection circuit 2 such as in the blood storage space 146 of the centrifuge 20 is transferred and stored in the air bag 27b. In the blood return process (blood component return process), the air stored in the air bag 27b is transferred into the blood storage space 146 of the centrifuge 20 and returned. Thereby, a predetermined blood component is returned to the donor.

  One end of the tube 42 is connected to the branch connector 22b, and the other end is connected to the airbag 27b.

  The intermediate bag (temporary storage bag) 27a, which is a blood component collection bag, is a container for temporarily storing concentrated platelets, that is, platelets including platelets (platelet component) (blood cell component) (first blood component) ( Storage part). One end of the tube 45 is connected to the branch connector 22c, and the other end is connected to the intermediate bag 27a.

  One end of the tube 46 is connected to the intermediate bag 27a, and a connecting branch connector 22e is provided at the other end. The other end of the tube 49 is connected to the branch connector 22e.

  In addition, one end of a tube 47 is connected to the branch connector 22e for connection, and a leukocyte removal filter (cell separation filter) (filtering) that separates and removes leukocytes (predetermined cells) from the concentrated platelets is provided in the middle of the tube 47. 261) is installed.

  The other end of the tube 47 is provided with a connecting branch connector 22g, and the other end of the tube 48, one end of which is connected to the platelet collection bag 26, is connected to the branch connector 22g.

  Further, a filter main body provided with a vent filter and a filter 22h provided with a cap are installed at the port of the branch connector 22g.

  Here, in a filtration operation for separating and removing leukocytes in the concentrated platelets described later, the tubes 46 and 47 constitute a supply tube for supplying the concentrated platelets from the intermediate bag 27a to the leukocyte removal filter 261. Constitutes a discharge tube for discharging the concentrated platelets after the white blood cells have been separated and removed from the white blood cell removal filter 261 (supplied to the platelet collection bag 26).

  That is, the tubes 46, 47, 48, the intermediate bag 27a, the leukocyte removal filter 261 and the platelet collection bag 26 constitute a filtration line for separating and removing leukocytes from the concentrated platelets.

  With the blood component collection device 1 assembled (when the blood component collection device 1 is used), the intermediate bag 27a, the leukocyte removal filter 261, the platelet collection bag 26, and the plasma collection bag 25 are each a plasma collection bag 25. Is set (positioned) at the highest position in the vertical direction, the intermediate bag 27a is positioned lower than the plasma collection bag 25 (vertically below), the leukocyte removal filter 261 is positioned lower than the intermediate bag 27a, and platelets The collection bag 26 is set (positioned) at a position lower than the leukocyte removal filter 261. The intermediate bag 27a is positioned higher than the blood storage space 146 of the rotor 142 of the centrifuge 20 (upward in the vertical direction), and the plasma collection bag 25 is separated from the blood storage space 146 of the rotor 142 of the centrifuge 20 with the blood storage space 146. Located at approximately the same height.

  The rank values of the intermediate bag 27 a, the leukocyte removal filter 261, the platelet collection bag 26, and the plasma collection bag 25 are not limited to this. For example, the plasma collection bag 25 is from the blood storage space 146 of the rotor 142 of the centrifuge 20. The second collection pump 12 may be provided, and the plasma collection bag 25 is located at a position lower than the intermediate bag 27a. Also good.

  In addition, the blood component collection apparatus 1 includes a hanger (hook) (not shown) that is a support unit that detachably supports the plasma collection bag 25, the intermediate bag 27a and the air bag 27b, and the platelet collection bag 26, respectively. Is provided. The plasma collection bag 25 and the intermediate bag 27a are respectively hooked and hung (suspended) on the corresponding hangers so that the outlet side (inlet side) is vertically downward. That is, when the blood component collection device 1 is used, the plasma collection bag 25 is provided so that the tubes 44 and 49 side thereof is vertically downward, and the intermediate bag 27a is provided such that the tubes 45 and 46 side thereof are in the vertical direction. It is provided so that it may become below.

  Further, as the leukocyte removal filter 261, for example, in a casing having an inlet and an outlet at both ends, for example, a woven fabric, a nonwoven fabric, a mesh, a foam or the like made of a synthetic resin such as polypropylene, polyester, polyurethane, polyamide, etc. The thing constituted by inserting the filtration member which laminated one layer or two layers or more of a porous body can be used.

  As the constituent materials of the tubes used for forming the first to third lines 21 to 23 and the pump tubes 21g, 22a and 23a, and the other tubes 41 to 51 and 21h, respectively, Vinyl chloride is preferred.

  If these tubes are made of polyvinyl chloride, sufficient flexibility and softness can be obtained, so that they are easy to handle and are suitable for clogging with a clamp or the like.

  Further, as the constituent materials of the branch connectors 21c, 21f, 22b, 22c, 22d, 22e, and 22g described above, the same materials as those described for the tube can be used.

  In addition, as each pump tube 21g, 22a, 23a, what has the intensity | strength of the grade which is not damaged even if it presses by each liquid feeding pump (for example, roller pump etc.) 11, 12, 13 mentioned later, respectively is used. Has been.

  Each of the plasma collection bag 25, the platelet collection bag 26, the intermediate bag 27a, the air bag 27b, and the bag 28 is laminated with a resin-made flexible sheet material, and the peripheral portions thereof are fused (thermal fusion, high frequency fusion). Or a bag formed by bonding with an adhesive or the like. As described above, the air bag 27b and the intermediate bag 27a are integrally formed (integrated).

  As a material used for each bag 25, 26, 27a, 27b, 28, for example, soft polyvinyl chloride is preferably used.

  In addition, as a sheet material used for the platelet collection bag 26, it is more preferable to use a material excellent in gas permeability in order to improve platelet storage stability.

  As such a sheet material, for example, polyolefin, DnDP plasticized polyvinyl chloride, or the like is used, and a sheet material of the above-described material is used without using such a material. What was thin (for example, about 0.1-0.5 mm, especially about 0.1-0.3 mm) is suitable.

  Although the main part of such a blood component collection circuit 2 is not shown, it is of a cassette type, for example. That is, the blood component collection circuit 2 partially stores each line (the first line 21, the second line 22, and the third line 23) and each predetermined tube, and partially holds them. In other words, it comprises a cassette housing in which they are partially fixed.

  In the cassette housing, both ends of the first pump tube 21g, both ends of the second pump tube 22a, and both ends of the third pump tube 23a are fixed. These pump tubes 21g, 22a, and 23a are respectively connected to the cassette housing. From the housing, it protrudes in the shape of a loop corresponding to the shape of each liquid feed pump (for example, roller pump etc.) 11, 12, and 13. Therefore, the first, second, and third pump tubes 21g, 22a, and 23a can be easily attached to the liquid feeding pumps 11, 12, and 13, respectively. The cassette housing is provided with respective flow path opening / closing means 81 to 86 described later.

  The centrifuge 20 provided in the blood component collection circuit 2 is generally called a centrifuge bowl, and separates blood into a plurality of blood components by centrifugal force.

  As shown in FIG. 2, the centrifuge 20 has a vertically extending tube 141 with an inlet 143 formed at the upper end, and rotates around the tube 141 and is liquid-tightly sealed with respect to the upper portion 145. And a hollow rotor 142.

  An annular blood storage space 146 is formed in the rotor 142 along the inner surface of the peripheral wall. The blood storage space 146 has a shape (tapered shape) in which the inner and outer diameters gradually decrease from the lower part toward the upper part in FIG. 2, and the lower part is formed in a substantially disc shape formed along the bottom part of the rotor 142. The upper end of the tubular body 141 communicates with the discharge port (outlet) 144. Further, in the rotor 142, the volume of the blood storage space 146 is, for example, about 100 to 350 mL, and the maximum inner diameter (maximum radius) from the rotating shaft of the rotor 142 is, for example, about 55 to 65 mm.

  Such a rotor 142 rotates under predetermined centrifugal conditions (rotation speed and rotation time) set in advance by the centrifuge drive device 10 included in the blood component collection device 1. Under this centrifugal condition, a blood separation pattern (for example, the number of blood components to be separated) in the rotor 142 can be set.

  In the present embodiment, as shown in FIG. 2, the centrifugal conditions are set so that the blood is separated from the inner layer into the plasma layer 131, the buffy coat layer 132, and the red blood cell layer 133 in the blood storage space 146 of the rotor 142.

Next, the overall configuration of the blood component collection device 1 shown in FIG. 1 will be described.
The blood component collection device 1 includes a centrifuge driving device 10 for rotating the rotor 142 of the centrifuge 20, a first liquid feeding pump 11 installed in the middle of the first line 21, and a tube 49. The second liquid delivery pump 12 installed in the middle, the third liquid delivery pump 13 installed in the middle of the third line 23, and the blood component collection circuit 2 (first line 21, tube 42, tube 44, tube 45, tube 47, tube 49), a plurality of flow path opening / closing means 81, 82, 83, 84, 85, 86 that can open and close the flow path, and display means for displaying (notifying) various information. (Notification means) and a display / operation section 17 which is an operation means for performing each operation, a storage section (storage means) 18, a centrifuge drive device 10, a first liquid feed pump 11, a second liquid feed pump 12. Third liquid pump 3, and a control section (control means) 3 for controlling the plurality of flow path shutter means 81 to 86, units such as the display and operation unit 17 and the storage unit 18.

  Furthermore, the blood component collection device 1 includes a turbidity sensor 14 mounted (installed) on the second line 22, an optical sensor 15 installed near the centrifuge 20, and a plurality of bubble sensors 31 to 36. And a weight sensor 16 for measuring the weight of the plasma together with the plasma collection bag 25.

  The control unit 3 includes three pump controllers (not shown) for the first liquid feeding pump 11, the second liquid feeding pump 12, and the third liquid feeding pump 13, and includes the control unit 3 and the first liquid feeding pump 13. The liquid feed pump 11, the second liquid feed pump 12, and the third liquid feed pump 13 are electrically connected via a pump controller.

A drive controller (not shown) included in the centrifuge drive device 10 is electrically connected to the control unit 3.
Each of the channel opening / closing means 81 to 86 is electrically connected to the control unit 3.

  The turbidity sensor 14, the optical sensor 15, the weight sensor 16, the bubble sensors 31 to 36, the display / operation unit 17, and the storage unit 18 are each electrically connected to the control unit 3.

  The control unit 3 is configured by, for example, a microcomputer (including a calculation unit, a memory, and the like). The control unit 3 includes the turbidity sensor 14, the optical sensor 15, the weight sensor 16, and the bubble sensors 31 to 36 described above. The detection signals from are input as needed. A signal (input) from the display / operation unit 17 is also input to the control unit 3.

  The control unit 3 collects blood components according to a preset program based on the detection signals from the turbidity sensor 14, the optical sensor 15, the weight sensor 16, the bubble sensors 31 to 36 and the signal from the display / operation unit 17. Control of the operation of each part of the apparatus 1, that is, rotation, stop, rotation direction (forward / reverse rotation), rotation speed (rotation speed) of each liquid feed pump 11, 12, 13 Control of opening / closing of the opening / closing means 81 to 86, operation of the centrifuge drive device 10, and driving of the display / operation unit 17 are controlled.

  The control unit 3 achieves (configures) the main function of the liquid feeding control means having a flow rate control function.

  The first flow path opening / closing means 81 is provided to open and close the first line 21 from the first pump tube 21g to the blood collection needle 29 side, that is, between the first pump tube 21g and the chamber 21d. Yes.

  The second flow path opening / closing means 82 is provided for opening and closing the tube 47. The third flow path opening / closing means 83 is provided for opening and closing the tube 44. The fourth flow path opening / closing means 84 is provided to open and close the tube 45. The fifth flow path opening / closing means 85 is provided for opening and closing the tube 42. The sixth flow path opening / closing means 86 is provided to open and close the tube 49 between the branch connector 22d and the branch connector 22e.

  Each flow path opening / closing means 81-86 includes an insertion part into which the first line 21 and tubes 47, 44, 45, 42, 49 can be inserted. The insertion part includes, for example, a solenoid, an electric motor, It has a clamp that operates with a drive source such as a cylinder (hydraulic or pneumatic). Specifically, an electromagnetic clamp that operates with a solenoid is suitable.

  Each of these channel opening / closing means (clamps) 81 to 86 operates based on a signal from the control unit 3.

  The display / operation unit 17 includes, for example, a touch panel including a liquid crystal display panel, an EL display panel, and the like. This display / operation unit 17 is used to input predetermined information and data (for example, blood count such as a target number of platelets (blood cell component) (target collection unit number), hematocrit value of donor (donor), etc.). Means are configured.

  In addition, a display unit (for example, a liquid crystal display panel, an EL display panel, etc.) that is a display unit (notification unit) for displaying (notifying) various information, and an operation unit (for example, an operation button) that is an operation unit for performing each operation , An operation switch, an operation dial, etc.) may be provided separately.

  The storage unit 18 includes a storage medium (also referred to as a recording medium) in which various information, data, tables, arithmetic expressions, programs, and the like are stored (also referred to as a recording medium). It is composed of various semiconductor memories, IC memories, and the like, such as volatile memories such as RAM, nonvolatile memories such as ROM, rewritable (erasable and rewritable) nonvolatile memories such as EPROM, EEPROM, and flash memory. Control such as writing (storage), rewriting, erasing, and reading in the storage unit 18 is performed by the control unit 3.

  As shown in FIG. 2, the centrifuge drive device 10 includes a housing 201 that houses the centrifuge 20, a leg portion 202, a motor 203 that is a drive source, and a disk-shaped fixing that holds the centrifuge 20. And a table 205.

  The housing 201 is placed and fixed on the upper portion of the leg portion 202. In addition, a motor 203 is fixed to the lower surface of the housing 201 via a spacer 207 with bolts 206.

  A fixed base 205 is fitted on the tip of the rotating shaft 204 of the motor 203 so as to rotate coaxially and integrally with the rotating shaft 204, and the bottom of the rotor 142 is fitted on the upper portion of the fixed base 205. A concave portion is formed.

  The upper portion 145 of the centrifuge 20 is fixed to the housing 201 by a fixing member (not shown).

  In such a centrifuge drive device 10, when the motor 203 is driven, the fixed base 205 and the rotor 142 fixed thereto rotate at, for example, about 3000 to 6000 rpm.

  The optical sensor 15 is installed on the side of the housing 201 (left side in FIG. 2).

  The optical sensor 15 is configured to project light toward the blood storage space 146 and to receive the reflected light.

  The optical sensor 15 irradiates (projects) light (for example, laser light) from the light projecting unit 151, and the reflected light reflected by the reflecting surface 147 of the rotor 142 is received by the light receiving unit 152. The light receiving unit 152 converts the received light quantity into an electrical signal.

  Here, the optical sensor 15 has a reflecting surface on one side and a reflecting plate 153 that changes the optical path, and the light irradiated from the light projecting unit 151 passes through the reflecting plate 153 and is reflected on the reflecting surface 147. The light that is irradiated to the light and reflected by the reflecting surface 147 is received by the light receiving unit 152 via the reflecting plate 153.

  At this time, the projection light and the reflected light are transmitted through the blood component in the blood storage space 146, but the position of the blood component interface (the interface B between the plasma layer 131 and the buffy coat layer 132 in this embodiment). Accordingly, since the abundance ratio of each blood component at a position where the light projection light and the reflected light are transmitted is different, the transmittance thereof is changed. As a result, the amount of light received by the light receiving unit 152 varies (changes), and this variation can be detected as a change in the output voltage from the light receiving unit 152.

  That is, the optical sensor 15 can detect the position of the blood component interface based on the change in the amount of light received by the light receiving unit 152.

  The interface of the blood component detected by the optical sensor 15 is not limited to the interface B, and may be the interface between the buffy coat layer 132 and the red blood cell layer 133, for example.

  Here, each of the layers 131 to 133 in the blood storage space 146 has a different color depending on the blood component, and in particular, the red blood cell layer 133 is red with the color of the red blood cells. For this reason, from the viewpoint of improving the accuracy of the optical sensor 15, there is a range suitable for the wavelength of the projection light, and the wavelength range is not particularly limited, but is preferably about 600 to 900 nm, for example. 750 to 800 nm is more preferable.

  The turbidity sensor 14 is for detecting the turbidity (platelet concentration) of the fluid flowing in the second line 22 and outputs a voltage value corresponding to the turbidity. Specifically, the turbidity sensor 14 outputs a low voltage value when the turbidity is high and a high voltage value when the turbidity is low.

  The turbidity sensor 14 can detect, for example, the concentration of platelets in plasma flowing through the second line 22, changes in the platelet concentration in plasma, and contamination of red blood cells into the plasma.

  Further, the bubble sensor 34 can detect, for example, the replacement of the fluid flowing in the second line 22 from air to plasma.

  As the turbidity sensor 14 and the bubble sensors 31 to 36, for example, an ultrasonic sensor, an optical sensor, an infrared sensor, or the like can be used.

  The first liquid feed pump 11 to which the first pump tube 21g is attached, the second liquid feed pump 12 to which the second pump tube 22a is attached, and the third pump to which the third pump tube 23a is attached. As the liquid feeding pump 13, for example, a non-blood contact type pump such as a roller pump is preferably used.

  Further, as the first liquid feeding pump (blood pump) 11, a pump capable of feeding blood in any direction is used. Specifically, a roller pump capable of forward rotation and reverse rotation is used.

  By the operation of the first liquid feeding pump 11, for example, liquid (fluid) such as blood and blood components in the first line 21 can be fed (transferred). Further, by the operation of the second liquid feeding pump 12, for example, liquid (fluid) in the plasma collection bag 25 such as plasma collected in the plasma collection bag 25 can be sent (transferred). Further, by the operation of the third liquid feed pump 13, for example, a liquid (fluid) such as an anticoagulant (anticoagulant liquid) in the third line 23 can be fed (transferred).

  Here, the blood component collection device 1 operates the first liquid feed pump 11 under the control of the control unit 3 to transfer (feed) the blood collected by the blood collection needle 29 into the blood storage space 146 of the centrifuge 20. And the plasma separated by the centrifuge 20 is collected in the plasma collection bag 25, and the second liquid feeding pump 12 is operated to collect the plasma in the plasma collection bag 25. A plasma circulation step of transferring the plasma into the blood storage space 146 of the centrifuge 20 via the plasma circulation line 24 and circulating the plasma between the plasma collection bag 25 and the blood storage space 146 of the centrifuge 20; Then, the second liquid feeding pump 12 is operated to transfer the plasma collected in the plasma collection bag 25 while accelerating it at a predetermined acceleration, and then transferred at a constant speed, and the blood storage space 146 ( Discharge port 1 of the rotor 142 4) Platelet collection process (blood component collection process) for temporarily storing (collecting) concentrated platelets (blood cell components) flowing out from the intermediate bag 27a and the remaining blood components (mainly red blood cells and white blood cells) to the donor A blood platelet collection operation (blood component collection operation) having a blood return step (blood component return step) to be returned is executed to perform component blood collection (see FIG. 3).

  In the plasma circulation process, the blood circulation space 24, the branch connector 21 f, the first centrifuge side line 21 b, and the blood storage space of the centrifuge 20, the main part of which is composed of the tube 49, the branch connector 22 d and the tube 50. A circulation circuit is formed (configured) by 146, the second line 22, the branch connector 22b, the tube 43, the branch connector 22c, the tube 44, and the plasma collection bag 25, and the second liquid feeding pump 12 With this operation, the plasma in the plasma collection bag 25 is transferred toward the blood storage space 146 of the centrifuge 20 through the plasma circulation line 24. Thereby, the plasma in the plasma collection bag 25 circulates in the circulation circuit (so as to pass through the blood storage space 146).

  By performing this plasma circulation step, it is possible to wash out platelets confined by the red blood cell layer or the like in the blood storage space 146 of the centrifugal separator 20 and to concentrate the blood cell layer (red blood cell layer and buffy coat layer) ( (Increase in internal viscosity) is suppressed (or prevented), and platelets can be well dispersed in the plasma within the buffy coat layer, thereby improving the recovery rate of platelets and improving the recovery rate. Variations can be suppressed.

  In component blood collection, this platelet collection operation (blood component collection operation) is performed at least once (one cycle), and usually a plurality of times (multiple cycles).

  In addition, the blood component collecting device 1 operates the first liquid feeding pump 11 to execute the plasma collecting process, and the second liquid feeding pump 12 is not interrupted without interrupting the plasma collecting process. Operated and configured to perform a plasma circulation process. That is, the first liquid delivery pump 11 and the second liquid delivery pump 12 are operated in time overlap (simultaneously), and the plasma collection process and plasma circulation process are overlapped in time (in parallel). ) It is supposed to run. As a result, it is possible to shorten the blood collection time as compared to alternately performing the plasma collection step and the plasma circulation step, thereby reducing the occupation time of the blood component collection device 1, and the burden on the donor. Can be reduced.

  In addition, the blood component collection device 1 executes the plasma circulation step alone after the plasma collection step is completed, and thereafter executes the platelet collection step.

  In the platelet collection step, the concentrated platelets may be collected in the intermediate bag 27a from when the plasma collected in the plasma collection bag 25 is transferred while being accelerated at a predetermined acceleration. After the transfer speed is made constant, concentrated platelets may be collected in the intermediate bag 27a.

  Further, after the plasma collection step is completed, the platelet collection step may be performed without performing the plasma circulation step.

  This blood component collection device 1 starts the plasma circulation step during the execution of the plasma circulation step, and when the processed blood volume reaches a predetermined amount (that is, when the processed blood volume increases by a predetermined amount), By changing (for example, decreasing) the liquid supply speed (liquid supply amount per unit time) of the second liquid supply pump 12, that is, the rotation speed (number of rotations), When the plasma in the plasma collection bag 25 is transferred (introduced) into the blood storage space 146 of the centrifuge 20 by the operation, that is, between the plasma collection bag 25 and the blood storage space 146 of the centrifuge 20 ( It is characterized in that it is configured to change (for example, decrease) the flow rate of plasma circulating in the circulation circuit (hereinafter also simply referred to as “plasma circulation flow rate”). In other words, when the first liquid feed pump 11 and the second liquid feed pump 12 are operating in a time-overlapping manner, the second liquid feed pump 11 is operated on the basis of the liquid feed amount of the first liquid feed pump 11. The blood circulation rate is changed by changing the liquid feeding speed of the liquid feeding pump 12. The processed blood volume corresponds to the liquid feed volume of the first liquid feed pump 11, and the number of rotations of the first liquid feed pump 11 and the liquid feed volume per one rotation of the first liquid feed pump 11. Based on the above, it can be obtained.

  The liquid feeding speed of the second liquid feeding pump 12 may be changed on the condition that the predetermined amount has been increased with reference to the plasma volume in the plasma collection bag 25.

By the way, the number of “1 unit” of platelets is 0.2 × 10 ¹¹ , and the following four types (1) to (4) are used as platelet preparations (those defined in the preparation standard). There is.

(1) 5 unit preparation The volume (volume) is 100 mL ± 20% and the number is 1.0 × 10 ^{11 to} 1.9 × 10 ¹¹ (2) 10 unit preparation The volume (volume) is 200 mL ± 20%, the number is 2.0 × 10 ^{11 to} 2.9 × 10 ¹¹ (3) 15 unit preparation The volume (volume) is 250 mL ± 20%, the number is 3.0 × 10 ^{11 to} 3.9 × 10 ¹¹ (4) 20 unit preparation The volume (volume) is 250 mL ± 20%, and the number is 4.0 × 10 ¹¹ or more.

  Next, the action (operation) of the blood component collection device 1, that is, the platelet collection operation (blood component collection operation) using the blood component collection device 1 will be described with reference to FIGS. 1, 3, and 4. FIG.

  3 and 4 are timing charts for explaining the operation of the blood component collection device shown in FIG. 1. FIG. 3 shows the operation in the first cycle, and FIG. 4 shows the second cycle and thereafter. The operation in each cycle is shown.

  3 and 4, the horizontal axis represents time, and the vertical axis represents the flow rate of the liquid (fluid) introduced into the blood storage space 146 of the centrifuge 20.

  3 and FIG. 4, the solid line indicates the liquid (fluid) introduced into the blood storage space 146 of the centrifuge 20 by the operation of the first liquid feeding pump 11, that is, blood (anticoagulant-added blood). ). The hatched portion is the flow rate of the blood cell component, and the non-hatched portion is the flow rate of the liquid component, that is, plasma (anticoagulant-added plasma).

  Also, the broken line (dotted line) in FIGS. 3 and 4 indicates the liquid component introduced into the blood storage space 146 of the centrifuge 20 by the operation of the second liquid feeding pump 12, that is, plasma (anticoagulant). (Added plasma) flow rate. In other words, the flow rate indicated by the broken line indicates that the plasma collected in the plasma collection bag 25 by the operation of the second liquid feeding pump 12 flows between the plasma collection bag 25 and the centrifuge 20 via the plasma circulation line 24 or the like. This is the plasma flow rate (circulation flow rate) when circulating between the blood storage space 146.

[0] First, the blood collection needle 29 of the third line 23 and the first line 21 to the chamber 21d is primed with an anticoagulant, and then the blood collection needle 29 is placed in the blood vessel of the donor (blood donor). Puncture.

[1] Platelet collection operation in the first cycle (see FIG. 3)
[11] First, a plasma collection step is performed. In the plasma collection step, blood is introduced into the blood storage space 146 of the rotor 142, and the plasma (PPP) separated by centrifuging the blood is collected in the plasma collection bag 25.

  Specifically, the first liquid supply pump 11 is operated at a predetermined rotational speed in a state where the first flow path opening / closing means 81 and the fifth flow path opening / closing means 85 are opened and the other flow path opening / closing means are closed. To start blood collection from the donor. The liquid feeding speed of the first liquid feeding pump 11 is preferably about 250 mL / min or less, and preferably about 40 to 150 mL / min (for example, 60 mL / min).

  Simultaneously with this blood collection, the third liquid feeding pump 13 is operated to supply an anticoagulant such as an ACD-A solution via the third line 23, and this anticoagulant is collected into the blood sample. Mix in.

  At this time, the rotation speed of the third liquid feeding pump 13 is controlled so that the anticoagulant is mixed at a predetermined ratio (preferably about 1/20 to 1/6, for example, 1/10) with respect to the collected blood. Is done.

  As a result, blood (anticoagulant-added blood) is transferred through the first line 21 and introduced into the blood storage space 146 of the rotor 142 through the tube 141 from the inlet 143 of the centrifuge 20.

  At this time, air (sterilized air) in the centrifuge 20 is sent into the air bag 27b through the second line 22 and the tube 42.

  Simultaneously with or before or after the blood collection, the centrifuge drive device 10 is operated to control the rotor 142 to rotate at a predetermined rotational speed.

  By the rotation of the rotor 142, the blood introduced into the blood storage space 146 is separated from the inside into three layers: a plasma layer (PPP layer) 131, a buffy coat layer (BC layer) 132, and a red blood cell layer (CRC layer) 133. The

  The rotational speed of the rotor 142 is preferably about 3000 to 6000 rpm, more preferably about 4200 to 5800 rpm. Further, in the following steps, the rotational speed of the rotor 142 is not changed unless otherwise specified.

  Further, when the blood collection and the supply of the anticoagulant are continued and blood (about 270 mL) exceeding the capacity of the blood storage space 146 is introduced into the blood storage space 146, the blood storage space 146 is filled with blood and centrifuged. Plasma (PPP) flows out from the outlet 144 of the vessel 20.

  At this time, the bubble sensor 34 installed in the second line 22 detects that the fluid flowing in the second line 22 has changed from air to plasma, and the control unit 3 detects the bubble sensor 34. Based on the signal, control is performed so that the fifth flow path opening / closing means 85 is closed and the third flow path opening / closing means 83 is opened.

  As a result, plasma is introduced and collected into the plasma collection bag 25 via the second line 22 and the tubes 43 and 44.

  The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 3.

  When the amount of plasma collected in the plasma collection bag 25 reaches the first amount that can execute the plasma circulation step, the plasma circulation step is started. The amount of plasma in the plasma collection bag 25 can be obtained based on the detection result of the weight sensor 16, that is, the information (weight signal) from the weight sensor 16.

  The first amount is an amount equal to or more than the minimum amount capable of performing the plasma circulation step, and is appropriately set according to various conditions, but is preferably about 10 to 50 g (10 to 52 mL), and is preferably 15 to 35 g. More preferably, it is about (15 to 36 mL).

  Specifically, the second liquid feeding pump 12 is operated (forward rotation) at a predetermined rotational speed, whereby the plasma in the plasma collection bag 25 is passed through the plasma circulation line 24 and the first line 21. The blood is introduced (transferred) into the blood storage space 146 at a constant speed, and the plasma flowing out from the outlet 144 of the centrifuge 20 is collected in the plasma collection bag 25 via the second line 22 and the tubes 43 and 44. That is, plasma is circulated at a constant speed between the plasma collection bag 25 and the blood storage space 146 of the centrifuge 20.

  Then, when the blood volume to be processed reaches a predetermined amount after the plasma circulation step is started during the execution of the plasma circulation step, the liquid feed rate of the second liquid feed pump 12 is decreased and the plasma circulation flow rate is reduced. Decrease.

  That is, at the initial stage of the plasma circulation process (a period from when the plasma circulation process is started until the processed blood volume reaches a predetermined amount), the liquid feeding speed of the second liquid feeding pump 12 is relatively high (high feeding speed). Set fluid velocity) and increase plasma circulation flow rate. Thereby, the effect by plasma circulation can be improved and the recovery rate of platelets can be further increased.

  In addition, after the processed blood volume reaches a predetermined amount, the liquid feeding speed of the second liquid feeding pump 12 is set to be relatively low (low liquid feeding speed), and the plasma circulation flow rate is reduced. Thereby, the amount of blood to be processed per cycle can be increased, and a large number of platelets can be collected in one cycle.

  In this way, the number of platelets collected per cycle is changed by changing the liquid feeding speed of the second liquid feeding pump 12 between the initial stage of the plasma circulation step and the time after the processed blood volume reaches a predetermined amount. (Collection amount) can be increased.

  The sum of the feeding speed of the second feeding pump 12 and the feeding speed of the plasma and the anticoagulant in the first feeding pump 11 in the plasma circulating process is the initial stage of the plasma circulating process (the plasma circulating process was started). In the period from when the processed blood volume reaches the predetermined amount), it is preferably about 1.05 to 1.5 times after the time when the processed blood volume reaches the predetermined amount. More preferably, it is about .35 times. Thereby, the collection rate of platelets can be increased while preventing the outflow of platelets from the outlet 144 of the centrifuge 20.

  Moreover, after the amount of blood to be processed reaches a predetermined amount, it is preferably about 40 to 140 mL / min, and more preferably about 50 to 130 mL / min. Thereby, the amount of blood to be processed per cycle can be increased while increasing the platelet recovery rate.

  It should be noted that the value after the treatment blood volume reaches the predetermined amount may be determined based on at least one of the target platelet unit number, donor hematocrit value, donor platelet concentration, allowable extracorporeal circulation rate, and the like. Good.

  Moreover, in this blood component collection device 1, the timing at which the blood circulation rate is decreased by decreasing the liquid feeding speed of the second liquid feeding pump 12 is set when the amount of blood to be processed reaches a predetermined amount. Thereby, platelets can be collected (collected) efficiently and stably.

  Further, in this blood component collection device 1, when the plasma circulation process is started, the processed blood volume is measured, and when the processed blood volume from the start of the plasma circulation process in the first cycle reaches a predetermined amount, The liquid feeding speed of the second liquid feeding pump 12 is decreased. The predetermined amount is appropriately set according to various conditions, but is preferably about 10 to 70 mL, and more preferably about 20 to 40 mL. Thereby, more platelets can be collected in one cycle.

  In the first cycle, the amount of plasma collected in the plasma collection bag 25 is rarely less than the first amount during the plasma circulation step, but the amount of plasma in the plasma collection bag 25 is small. When the amount is less than the first amount, the plasma circulation process is interrupted as in the second cycle described later, and the plasma circulation process is resumed when the amount of plasma in the plasma collection bag 25 reaches the first amount. To do.

  In the plasma collection process and the plasma circulation process, as the amount of red blood cells in the blood storage space 146 increases, that is, the layer thickness of the red blood cell layer 133 increases, the interface B gradually moves in the direction of the rotation axis of the rotor 142.

  Next, when the interface B between the plasma layer 131 and the buffy coat layer 132 reaches the first position, the first liquid feeding pump 11 and the third liquid feeding pump 13 are stopped, and the plasma collecting step is terminated. The first flow path opening / closing means 81 is closed, and the plasma circulation step is executed alone.

  Whether or not the interface B has reached the first position can be determined based on a detection signal (interface position detection information) from the optical sensor 15. The first position of the interface B is preferably the position at which the detection signal from the optical sensor 15 (output voltage from the light receiving unit 152) is about 1 to 2V.

  In the plasma circulation step performed independently, the liquid feeding speed of the second liquid feeding pump 12 is preferably about 70 to 110 to 150 mL / min, and more preferably about 90 to 110 mL / min.

[12] Next, a platelet collecting step is performed. In the platelet collection step, the second liquid feeding pump 12 is operated without collecting the plasma in the collection bag 25, and the plasma in the plasma collection bag 25 is moved into the blood storage space 146 at the first acceleration. Circulate while accelerating, then change to a second acceleration greater than the first acceleration, circulate while accelerating at this second acceleration to allow platelets to flow out of the blood reservoir space 146, etc. Circulating at high speed, the platelet concentrate is collected (stored) in the intermediate bag 27a.

When collecting concentrated platelets in the intermediate bag 27a, the third channel opening / closing means 83 is closed, the fourth channel opening / closing means 84 is opened, and the concentrated platelets that have flowed out from the outlet 144 of the centrifuge 20 are opened. Is introduced into the intermediate bag 27a through the second line 22 and the tubes 43 and 45 and collected (stored). At this time, since the second flow path opening / closing means 82 and the sixth flow path opening / closing means 86 are closed, the concentrated platelets do not flow out of the intermediate bag 27a.
Note that the circulation by the first acceleration can be omitted.

  Next, the fifth flow path opening / closing means 85 is opened, all other flow path opening / closing means 81 to 84, 86 are closed, the second liquid feeding pump 12 is stopped, and the platelet collecting step Exit.

[13] Next, the centrifuge 20 (centrifuge drive device 10) is stopped, and a blood return process is performed. In the blood return process, blood components (remaining blood components) in the blood storage space 146 of the rotor 142 are returned.

  Specifically, the first flow path opening / closing means 81 and the fifth flow path opening / closing means 85 are opened, and the first liquid feed pump 11 is operated (reversely rotated) at a predetermined rotational speed. The liquid feeding speed of the first liquid feeding pump 11 is preferably about 20 to 120 mL / min or less (for example, 90 mL / min).

  As a result, blood components (mainly red blood cells and white blood cells) remaining in the blood storage space 146 of the rotor 142 are discharged from the inlet 143 of the centrifuge 20 and are passed through the first line 21 (blood collection needle 29). Blood is returned (returned) to the donor.

Then, after the air discharged from the centrifugal separator 20 is detected by the bubble sensor 32 and the first liquid feeding pump 11 is rotated a predetermined number of times, the first flow path opening / closing means 81 and the fifth flow path are While closing the opening-closing means 85, the 1st liquid feeding pump 11 is stopped, and the blood return process is complete | finished.
Thereby, the platelet collection operation in the first cycle is completed.

[2] Platelet collection operation in the second cycle that is not the final cycle (see FIG. 4)
Hereinafter, the platelet collection operation in the second cycle will be described focusing on the differences from the first cycle described above, and the description of the same matters will be omitted.

  When the platelet collection operation of the first cycle is completed, the plasma collection bag 25 normally collects plasma in an amount equal to or greater than the first amount (a sufficiently larger amount than the first amount). In the platelet collection operation of the second cycle, the plasma collection process and the plasma circulation process are started almost simultaneously. If the amount of plasma collected in the plasma collection bag 25 becomes less than the first amount during the plasma circulation step, the plasma circulation step is interrupted, and then collected in the plasma collection bag 25. When the amount of plasma reached the first amount, the plasma circulation process is resumed.

  In addition, the liquid feeding speed of the second liquid feeding pump 12 before interrupting the plasma circulation process and the liquid feeding speed of the second liquid feeding pump 12 after resuming the plasma circulation process are set to different values. (The liquid feeding speed of the second liquid feeding pump 12 after restarting is increased more than before the plasma circulation process is interrupted).

  That is, in the plasma circulation step, first, the liquid feeding speed of the second liquid feeding pump 12 is set to be relatively low (low liquid feeding speed). As a result, the amount of decrease in plasma in the plasma collection bag is reduced, so that the plasma circulation process is interrupted in the middle of the plasma circulation process because the amount of plasma in the plasma collection bag becomes less than the first amount. The probability that it will end up can be reduced.

  Therefore, when the plasma circulation process ends without being interrupted, the liquid feeding speed of the second liquid feeding pump 12 remains relatively low (low liquid feeding speed), and the plasma circulation flow rate is small. Since the circulation process can be performed continuously, it is possible to suppress or prevent a decrease in the recovery rate of platelets.

  On the other hand, when the plasma circulation process is interrupted in the middle, the liquid feeding speed of the second liquid feeding pump 12 is relatively low during the period from the start of the plasma circulation process to the interruption of the plasma circulation process ( After the time when the amount of plasma collected in the plasma collection bag 25 reaches the first amount and the plasma circulation process is restarted, the second feeding pump 12 feeds. Set the fluid velocity relatively high (high fluid delivery rate) and increase plasma circulation flow rate. As a result, the effect of plasma circulation is improved, and the blood cell layer concentrated in the blood storage space 146 of the centrifugal separator 20 by interruption of the plasma circulation process can be reliably returned to the original state before being concentrated. Thus, the recovery rate of platelets can be increased.

  The preferred values of the low liquid feed rate and the high liquid feed rate of the second liquid feed pump 12 in the plasma circulation step are the low liquid feed rate and the high liquid feed rate of the second liquid feed pump 12 in the first cycle described above, respectively. Same as speed.

  The platelet collection operation in each cycle after the third cycle, which is not the final cycle, is the same as the platelet collection operation in the first cycle, but when the second cycle is completed, the plasma collected in the plasma collection bag 25 is collected. Since the amount is relatively large, the amount of plasma collected in the plasma collection bag 25 is rarely less than the first amount in each cycle after the third cycle.

[3] Platelet collection operation in the final cycle Hereinafter, the platelet collection operation in the final cycle will be described with a focus on differences from the second cycle described above, and description of similar matters will be omitted.

  In the platelet collection operation of the final cycle, in the blood return process, the air discharged from the centrifuge 20 is detected by the bubble sensor 32, and the air return process is terminated when air is further detected by the bubble sensor 35 or 36. This is the same as the platelet collection operation in the second cycle described above.

  Further, in this blood component collection device 1, in parallel with performing the final cycle of platelet collection operation, or after completion of the final cycle of platelet collection operation, it is temporarily stored in the intermediate bag 27a under the control of the control unit 3. Concentrated platelets collected (stored) are supplied to a leukocyte removal filter 261, and filtration of concentrated platelets, that is, a filtration operation (filtration step) for separating and removing leukocytes in the concentrated platelets is performed.

  In this filtering operation, the second flow path opening / closing means 82 is opened. Thereby, the concentrated platelets in the intermediate bag 27a are transferred into the platelet collection bag 26 through the tubes 46 and 47, the leukocyte removal filter 261 and the tube 48 due to a drop (self-weight). At this time, most of the concentrated platelets pass through the filtration member of the leukocyte removal filter 261, but the leukocytes are captured by the filtration member. For this reason, the removal rate of leukocytes in the platelet preparation can be made extremely high.

  The timing for starting this filtration operation is not particularly limited, but from the viewpoint of shortening the donor's restraint time, this filtration operation is performed simultaneously with the platelet collection operation of the final cycle (particularly in the early stages of the platelet collection operation). It is preferable to start.

  The transfer of the concentrated platelets from the intermediate bag 27a to the platelet collection bag 26 may be performed using a pump.

  Next, the control operation of the control unit 3 in the platelet collection operation (blood component collection operation) using the blood component collection device 1 will be described with reference to the flowcharts shown in FIGS. The control operation in the plasma circulation process will be mainly described.

  In the first cycle of platelet collection operation, as shown in FIG. 5, first, a flag (i) indicating the operation / stop of the second liquid feeding pump 12 is set to “0” (i = 0) (step S101). A flag i = 0 indicates a state where the second liquid feed pump 12 is stopped, and a flag i = 1 indicates a state where the second liquid feed pump 12 is operating.

  Next, based on the detection signal (interface position detection information) from the optical sensor 15, it is determined whether the interface B has reached the first position (predetermined level) (step S102).

  If the interface B has not reached the first position in step S102, it is determined whether or not the amount of plasma collected in the plasma collection bag 25 is equal to or greater than the first amount (step S103). ).

  In step S103, when the amount of plasma collected in the plasma collection bag 25 is less than the first amount, the flag i = 0 is set while the second liquid pump 12 is stopped (step S104). The setting is made (step S105), the process returns to step S102, and step S102 and subsequent steps are executed again.

  In step S103, when the amount of plasma collected in the plasma collection bag 25 is equal to or greater than the first amount, it is possible to execute the plasma circulation step.

  Next, it is determined whether or not the flag i = 1 (step S106). If the flag i = 0, the second liquid feed pump 12 is operated and rotated at a high speed (step S107). The liquid feeding speed of the second liquid feeding pump 12 is set to a high liquid feeding speed, flag i = 1 is set (step S108), the process returns to step S102, and step S102 and subsequent steps are executed again.

  In step S106, if the flag i = 1, it is determined whether or not the processed blood volume is equal to or greater than a predetermined volume (step S109). If the processed blood volume is less than the predetermined volume, The operation (high speed rotation) of the liquid feed pump 12 is continued (step S107), the flag i = 1 is set (step S108), the process returns to step S102, and step S102 and subsequent steps are executed again.

  In step S109, when the processed blood volume is equal to or larger than the predetermined amount, the rotation speed of the second liquid feed pump 12 is changed to a low speed (step S110), that is, the liquid feed of the second liquid feed pump 12 is changed. The speed is set to a low liquid feeding speed, the process returns to step S102, and step S102 and subsequent steps are executed again.

  In step S102, when the interface B reaches the first position, the first liquid feeding pump 11 is stopped, the plasma collection process is terminated, and the plasma circulation process is executed alone (step S111). ).

  Next, a platelet collection process is executed (step S112), and a blood return process is executed (step S113).

  As described above, the plasma circulation process having the pattern indicated by the broken line in FIG. 3 can be executed.

  In the platelet collection operation in each cycle after the second cycle, as shown in FIG. 6, first, the flag (i) indicating the operation / stop of the second liquid feeding pump 12 is set to “1” (i = 1) (Step S201). A flag i = 0 indicates a state where the second liquid feed pump 12 is stopped, and a flag i = 1 indicates a state where the second liquid feed pump 12 is operating.

  Next, a flag (j) indicating that the liquid feeding speed of the second liquid feeding pump 12 is high / low liquid feeding speed is set to “0” (j = 0) (step S202). The flag j = 0 indicates a state where the liquid feeding speed of the second liquid feeding pump 12 is set to a low liquid feeding speed, and the flag j = 1 indicates that the liquid feeding speed of the second liquid feeding pump 12 is high. This shows the state where the liquid feed speed is set.

  Next, based on the detection signal (interface position detection information) from the optical sensor 15, it is determined whether the interface B has reached the first position (predetermined level) (step S203).

  In step S203, if the interface B has not reached the first position, it is determined whether or not the amount of plasma collected in the plasma collection bag 25 is equal to or greater than the first amount (step S204). ).

  In step S204, if the amount of plasma collected in the plasma collection bag 25 is greater than or equal to the first amount, it is possible to execute the plasma circulation step and determine whether or not flag i = 1. Determination is made (step S207). If flag i = 1, it is determined whether flag j = 1 (step S211). If flag j = 0, the second liquid feed pump 12 is turned on. Operate and rotate at low speed (step S212), that is, set the liquid feeding speed of the second liquid feeding pump 12 to a low liquid feeding speed, return to step S203, and execute step S203 and subsequent steps again.

  Here, when the plasma circulation process is executed, the amount of plasma in the plasma collection bag 25 gradually decreases until plasma begins to be introduced into the plasma collection bag 25 in the plasma collection process.

  In step S204, when the amount of plasma collected in the plasma collection bag 25 is less than the first amount, the second liquid feeding pump 12 is stopped (step S205), and the flag i = 0 is set. (Step S206), returning to Step S203, Step S203 and the subsequent steps are executed again.

  Here, when the plasma collection step is executed, the amount of plasma in the plasma collection bag 25 gradually increases.

  In step S204, if the amount of plasma collected in the plasma collection bag 25 is greater than or equal to the first amount, it is possible to execute the plasma circulation step and determine whether or not flag i = 1. Judgment is made (step S207), and if the flag i = 0, the second liquid feed pump 12 is operated and rotated at a high speed (step S208), that is, the liquid feed speed of the second liquid feed pump 12 is set. The high liquid feeding speed is set, the flag i = 1 is set (step S209), the flag j = 1 is set (step S210), the process returns to step S203, and step S203 and subsequent steps are executed again.

  In step S207, if flag i = 1, it is determined whether flag j = 1 (step S211). If flag j = 1, the operation of the second liquid feed pump 12 is performed. (High-speed rotation) is continued (step S208), the flag i = 1 is set (step S209), the flag j = 1 is set (step S210), the process returns to step S203, and step S203 and subsequent steps are executed again.

  In step S203, when the interface B reaches the first position, the first liquid pump 11 is stopped, the plasma collection process is terminated, and the plasma circulation process is executed alone (step S213). ).

  Next, a platelet collection process is executed (step S214), and a blood return process is executed (step S215).

  As described above, when the plasma collection process is interrupted in the middle, the plasma circulation process having the pattern shown by the broken line in FIG. 4 can be executed. When the plasma collection process is not interrupted, the plasma at a low liquid feeding speed can be performed. A circulation step can be performed.

  As described above, according to this blood component collection device, the blood volume to be processed can be set appropriately without excess and deficiency, and the plasma circulation step is executed in a time-overlapping manner (simultaneously) with the plasma collection step. Can be prevented from being executed or interrupted. Thereby, platelets can be collected efficiently and stably.

Second Embodiment
FIG. 7 is a timing chart for explaining the operation in the second embodiment of the blood component collection device of the present invention. FIG. 7 shows the operation in each cycle after the second cycle.

  Hereinafter, the blood component collection device 1 according to the second embodiment will be described with a focus on differences from the first embodiment described above, and descriptions of similar matters will be omitted.

  The blood component collection device 1 of the second embodiment is the same as that of the first embodiment described above except that the plasma circulation process in the platelet collection operation of each cycle after the second cycle is different.

  As shown in FIG. 7, in the blood component collection device 1 of the second embodiment, the control unit 3 is collected in the plasma collection bag 25 during the plasma circulation process in each cycle after the second cycle. Whether or not the amount of plasma is less than the first amount is predicted based on the hematocrit value (blood count) of the donor. Therefore, the main functions of the liquid feeding control means and the prediction means are achieved (configured) by the control unit 3.

  The prediction is preferably performed before starting the plasma circulation process of this cycle (current cycle). For example, when this cycle is the second cycle, it is performed in parallel with the blood return process of the first cycle. It is preferred to do so. However, it goes without saying that the prediction may be performed immediately after the start of the plasma circulation step of this cycle.

  When the control unit 3 predicts that in each cycle after the second cycle, the amount of plasma collected in the plasma collection bag 25 during the plasma circulation step is less than the first amount If the amount of plasma collected in the plasma collection bag 25 falls below a second amount that is larger than the first amount during the plasma circulation step, the second liquid feed pump 12 feeds. When the liquid velocity is increased and then the amount of plasma collected in the plasma collection bag 25 becomes less than the first amount, the plasma circulation process is interrupted, and then the amount of plasma collected in the plasma collection bag 25 is changed to the first amount. When the amount of 1 is reached, the plasma circulation process is resumed.

  In addition, the liquid feeding speed of the second liquid feeding pump 12 after resuming the plasma circulating process is made lower than the liquid feeding speed of the second liquid feeding pump 12 before the plasma circulation process is interrupted.

  That is, in the plasma circulation step, regardless of whether or not the amount of plasma collected in the plasma collection bag 25 during the execution of the plasma circulation step is predicted to be less than the first amount, The liquid feeding speed of the second liquid feeding pump 12 is set to be relatively low (low liquid feeding speed). As a result, the amount of decrease in plasma in the plasma collection bag is reduced, so that the plasma circulation process is interrupted in the middle of the plasma circulation process because the amount of plasma in the plasma collection bag becomes less than the first amount. The probability that it will end up can be reduced.

  Therefore, when the plasma circulation process ends without being interrupted, the liquid feeding speed of the second liquid feeding pump 12 remains relatively low (low liquid feeding speed), and the plasma circulation flow rate is small. Since the circulation process can be performed continuously, it is possible to suppress or prevent a decrease in the recovery rate of platelets.

  In addition, when it is predicted that the amount of plasma collected in the plasma collection bag 25 during the plasma circulation process is less than the first amount, the plasma circulation process is being performed. When the amount of plasma collected in the plasma collection bag 25 is equal to or less than the second amount (> first amount), the liquid feeding speed of the second liquid feeding pump 12 is relatively high (high liquid feeding speed). Set and increase plasma circulation flow rate. As a result, the effect of plasma circulation is improved, and even if the plasma circulation process is interrupted, the blood cell layer in the blood storage space 146 of the centrifuge 20 becomes difficult to concentrate, thereby increasing the platelet recovery rate. .

  And when the plasma circulation process is interrupted in the middle, after the amount of plasma collected in the plasma collection bag 25 reaches the first amount and the plasma circulation process is resumed, the second liquid feeding pump No. 12 is set to a relatively low rate (low rate) and the plasma circulation flow rate is reduced. Thereby, the amount of blood to be processed per cycle can be increased, and a large number of platelets can be collected in one cycle.

  Here, the prediction (judgment) of whether or not the amount of plasma collected in the plasma collection bag 25 during the plasma circulation step is less than the first amount is performed as follows.

  That is, when the following (formula 1) is not established, it is predicted that the amount of plasma collected in the plasma collection bag 25 during the plasma circulation process will be less than the first amount, and (formula 1) is If established, it is predicted that the amount of plasma collected in the plasma collection bag 25 during the plasma circulation process will not be less than the first amount.

Predicted plasma volume ≧ plasma volume in plasma collection bag 25 (1 set)
However, the estimated plasma volume and the plasma volume in the plasma collection bag 25 are obtained from the following (formula 2) and (formula 3), respectively.
Predicted blood plasma volume = A−B × Ht value (Expression 2)
A, B: values obtained by experiments (in this embodiment, for example, A = 530, B = 8)
Ht value: Donor's hematocrit value Plasma volume in plasma collection bag 25 = liquid feeding speed of second liquid feeding pump 12 x t + first quantity (Expression 3)
Q _PP = Q _CF -Q _BP -Q _ACD (Equation 3-1)
Q _PP [mL / min]: Liquid feeding speed of the second liquid feeding pump 12 (in this embodiment, 50 to 130 is set)
Q _CF [mL / min]: Critical flow speed Q _ACD [mL / min]: Liquid feed speed of the third liquid feed pump 13 = Q _B / (X + 1)
Q _BB [mL / min]: Blood sampling rate of blood cell component = (Q _B −Q _ACD ) / Ht value Q _BP [mL / min]: Blood sampling rate of plasma component = (Q _B −Q _ACD ) / (1-Ht value)
Q _B [mL / min]: Blood collection speed including anticoagulant t = V _B / (Q _CF + Q _BB ) (3-2)
t [min]: Time from the start of the plasma collection process until the plasma flows out of the centrifuge 20 X: Blood ratio in anticoagulant-added blood V _B [mL]: Volume of the centrifuge 20

  Next, the control operation of the control unit 3 in the platelet collection operation (blood component collection operation) using the blood component collection device 1 will be described with reference to the flowcharts shown in FIGS. 1 and 8. The control operation in the plasma circulation process will be mainly described.

  In the platelet collection operation of each cycle after the second cycle, as shown in FIG. 8, it is first determined whether or not the interruption of the plasma circulation process is predicted (step S301).

  Here, in the middle of executing the plasma circulation step, whether or not the amount of plasma collected in the plasma collection bag 25 is less than the first amount is predicted in parallel with the blood return step of the previous cycle. In step S301, based on the result, it is determined whether or not the interruption of the plasma circulation process is predicted.

  When it is predicted that the amount of plasma collected in the plasma collection bag 25 will be less than the first amount during the execution of the plasma circulation process, it is predicted that the plasma circulation process will be interrupted in the middle, A flag (k) indicating that interruption of the circulation process is predicted / “non-interruption (not interrupted) is predicted” is set to “1” (k = 1) (step S302). The flag k = 1 indicates “interruption of the plasma circulation process is predicted”, and k = 0 indicates “the plasma circulation process is predicted not to be interrupted”.

  Further, when it is predicted that the amount of plasma collected in the plasma collection bag 25 during the plasma circulation process is not less than the first amount, it is predicted that the plasma circulation process will not be interrupted, and the flag ( k) is set to “0” (k = 0) (step S303).

  Next, based on the detection signal (interface position detection information) from the optical sensor 15, it is determined whether the interface B has reached the first position (predetermined level) (step S304).

  If the interface B has not reached the first position in step S304, it is determined whether or not the amount of plasma collected in the plasma collection bag 25 is equal to or greater than the first amount (step S305). ).

  In step S305, if the amount of plasma collected in the plasma collection bag 25 is greater than or equal to the first amount, it is possible to execute the plasma circulation step and determine whether or not the flag k = 1. Judgment is made (step S308), and if the flag k = 0, the second liquid feed pump 12 is operated and rotated at a low speed (step S311). The low liquid feeding speed is set, the process returns to step S304, and step S304 and subsequent steps are executed again.

  In step S308, when the flag k = 1, it is determined whether or not the amount of plasma collected in the plasma collection bag 25 is equal to or less than the second amount (> first amount) ( Step S309), when the amount of plasma collected in the plasma collection bag 25 is larger than the second amount, the second liquid feeding pump 12 is operated and rotated at a low speed (Step S311), that is, The liquid feeding speed of the second liquid feeding pump 12 is set to a low liquid feeding speed, the process returns to step S304, and step S304 and subsequent steps are executed again.

  Here, when the plasma circulation process is executed, the amount of plasma in the plasma collection bag 25 gradually decreases until plasma begins to be introduced into the plasma collection bag 25 in the plasma collection process.

  In step S309, when the amount of plasma collected in the plasma collection bag 25 is equal to or less than the second amount, the rotation speed of the second liquid feeding pump 12 is changed to a high speed (step S310), that is, the first The liquid feeding speed of the second liquid feeding pump 12 is set to a high liquid feeding speed, the process returns to step S304, and step S304 and subsequent steps are executed again.

  In step S305, when the amount of plasma collected in the plasma collection bag 25 is less than the first amount, the second liquid feeding pump 12 is stopped (step S306), and the flag k = 0 is set. (Step S307), the process returns to Step S304, and Steps S304 and after are executed again.

  Here, when the plasma collection step is executed, the plasma flows out from the blood storage space 146 of the centrifuge 20, and the amount of plasma in the plasma collection bag 25 gradually increases.

  In step S305, if the amount of plasma collected in the plasma collection bag 25 is greater than or equal to the first amount, it is possible to execute the plasma circulation step and determine whether or not the flag k = 1. Judgment is made (step S308), and if the flag k = 1, the second liquid feed pump 12 is operated and rotated at a low speed (step S311). The low liquid feeding speed is set, the process returns to step S304, and step S304 and subsequent steps are executed again.

  In step S304, when the interface B reaches the first position, the first liquid pump 11 is stopped, the plasma collection process is terminated, and the plasma circulation process is executed alone (step S312). ).

  Next, a platelet collection process is executed (step S313), and a blood return process is executed (step S314).

As described above, when the plasma collection process is interrupted in the middle, the plasma circulation process of the pattern shown by the broken line in FIG. 7 can be executed. When the plasma collection process is not interrupted, the plasma at a low liquid feeding speed can be performed. A circulation step can be performed.
According to the blood component collection device 1, the same effect as that of the first embodiment described above can be obtained.

<Third Embodiment>
FIG. 9 is a timing chart for explaining the operation in the ninth embodiment of the blood component collection device of the present invention. FIG. 9 shows the operation in each cycle after the second cycle.

  Hereinafter, the blood component collection device 1 according to the third embodiment will be described with a focus on differences from the second embodiment described above, and description of similar matters will be omitted.

  The blood component collection device 1 of the third embodiment is the same as that of the second embodiment described above except that the plasma circulation process in the platelet collection operation of each cycle after the second cycle is different.

  As shown in FIG. 9, in the blood component collection device 1 of the third embodiment, the control unit 3 is collected in the plasma collection bag 25 during the plasma circulation process in each cycle after the second cycle. If the amount of plasma is predicted to be less than the first amount, the amount of plasma collected in the plasma collection bag 25 during the plasma circulation step is more than the first amount. When the amount is less than or equal to the larger second amount, the liquid feeding speed of the second liquid feeding pump 12 is increased, and then when the amount of plasma collected in the plasma collection bag 25 is less than the first amount, the plasma circulation step After that, when the amount of plasma collected in the plasma collection bag 25 reaches the first amount, the plasma circulation process is resumed.

  When the amount of blood to be processed reaches a predetermined amount, the liquid feeding speed of the second liquid feeding pump 12 is reduced to reduce the plasma circulation flow rate.

  In addition, the liquid feeding speed of the second liquid feeding pump 12 before the plasma circulation process is interrupted and the liquid feeding speed of the second liquid feeding pump 12 after the plasma circulation process is resumed are made substantially the same.

  That is, in the plasma circulation step, regardless of whether or not the amount of plasma collected in the plasma collection bag 25 during the execution of the plasma circulation step is predicted to be less than the first amount, The liquid feeding speed of the second liquid feeding pump 12 is set to be relatively low (low liquid feeding speed). As a result, the amount of decrease in plasma in the plasma collection bag is reduced, so that the plasma circulation process is interrupted in the middle of the plasma circulation process because the amount of plasma in the plasma collection bag becomes less than the first amount. The probability that it will end up can be reduced.

  Therefore, when the plasma circulation process ends without being interrupted, the liquid feeding speed of the second liquid feeding pump 12 remains relatively low (low liquid feeding speed), and the plasma circulation flow rate is small. Since the circulation step can be performed continuously, it is possible to suppress or prevent a reduction in the platelet recovery rate.

  In addition, when it is predicted that the amount of plasma collected in the plasma collection bag 25 during the plasma circulation process is less than the first amount, the plasma circulation process is being performed. When the amount of plasma collected in the plasma collection bag 25 is equal to or less than the second amount (> first amount), the liquid feeding speed of the second liquid feeding pump 12 is relatively high (high liquid feeding speed). Set and increase plasma circulation flow rate. As a result, the effect of plasma circulation is improved, and even if the plasma circulation process is interrupted, the blood cell layer in the blood storage space 146 of the centrifuge 20 becomes difficult to concentrate, thereby increasing the platelet recovery rate. .

  And when the plasma circulation process is interrupted in the middle, after the amount of plasma collected in the plasma collection bag 25 reaches the first amount and the plasma circulation process is resumed, the second liquid feeding pump 12 is set to a relatively high (high liquid supply speed) and the plasma circulation flow rate is increased. Thereby, the effect by plasma circulation can be improved and the recovery rate of platelets can be further increased.

  In addition, after the processed blood volume reaches a predetermined amount, the liquid feeding speed of the second liquid feeding pump 12 is set to be relatively low (low liquid feeding speed), and the plasma circulation flow rate is reduced. Thereby, the amount of blood to be processed per cycle can be increased, and a large number of platelets can be collected in one cycle.

  Next, the control operation of the control unit 3 in the platelet collection operation (blood component collection operation) using the blood component collection device 1 will be described with reference to the flowcharts shown in FIGS. 1 and 10. The control operation in the plasma circulation process will be mainly described.

  In the platelet collection operation in each cycle after the second cycle, as shown in FIG. 10, first, the flag (i) indicating the operation / stop of the second liquid feeding pump 12 is set to “1” (i = 1) (Step S401). A flag i = 0 indicates a state where the second liquid feed pump 12 is stopped, and a flag i = 1 indicates a state where the second liquid feed pump 12 is operating.

  Next, a flag (j) indicating that the liquid feeding speed of the second liquid feeding pump 12 indicates a high liquid feeding speed / a low liquid feeding speed is set to “0” (j = 0) (step S402). The flag j = 0 indicates a state where the liquid feeding speed of the second liquid feeding pump 12 is set to a low liquid feeding speed, and the flag j = 1 indicates that the liquid feeding speed of the second liquid feeding pump 12 is high. This shows the state where the liquid feed speed is set.

Next, it is determined whether or not the interruption of the plasma circulation process is predicted (step S403).
Here, in the middle of executing the plasma circulation step, whether or not the amount of plasma collected in the plasma collection bag 25 is less than the first amount is predicted in parallel with the blood return step of the previous cycle. In step S403, it is determined based on the result whether or not the interruption of the plasma circulation process is predicted.

  When it is predicted that the amount of plasma collected in the plasma collection bag 25 will be less than the first amount during the execution of the plasma circulation process, it is predicted that the plasma circulation process will be interrupted in the middle, A flag (k) indicating that the interruption of the circulation process is predicted "/" not interrupted (not interrupted) is predicted "is set to" 1 "(k = 1) (step S404). The flag k = 1 indicates “interruption of the plasma circulation process is predicted”, and k = 0 indicates “the plasma circulation process is predicted not to be interrupted”.

  Further, when it is predicted that the amount of plasma collected in the plasma collection bag 25 during the plasma circulation process is not less than the first amount, it is predicted that the plasma circulation process will not be interrupted, and the flag ( k) is set to “0” (k = 0) (step S405).

  Next, based on the detection signal (interface position detection information) from the optical sensor 15, it is determined whether or not the interface B has reached the first position (predetermined level) (step S406).

  If the interface B has not reached the first position in step S406, it is determined whether or not the amount of plasma collected in the plasma collection bag 25 is equal to or greater than the first amount (step S407). ).

  In step S407, if the amount of plasma collected in the plasma collection bag 25 is greater than or equal to the first amount, it is possible to execute the plasma circulation step and determine whether or not flag k = 1. A determination is made (step S411). If the flag k = 0, it is determined whether the flag i = 1 (step S413). If the flag i = 1, the flag j = 1. (Step S414), and when the flag j = 0, the second liquid feeding pump 12 is operated and rotated at a low speed (step S419), that is, the liquid feeding of the second liquid feeding pump 12 is performed. The speed is set to a low liquid feed speed, flag j = 0 is set (step S420), the process returns to step S406, and step S406 and subsequent steps are executed again.

  In step S411, if the flag k = 1, it is determined whether or not the amount of plasma collected in the plasma collection bag 25 is equal to or less than the second amount (> first amount) ( Step S412) When the amount of plasma collected in the plasma collection bag 25 is larger than the second amount, the second liquid feed pump 12 is operated and rotated at a low speed (Step S419), that is, The liquid feeding speed of the second liquid feeding pump 12 is set to the low liquid feeding speed, the flag j = 0 is set (step S420), the process returns to step S406, and step S406 and subsequent steps are executed again.

  Here, when the plasma circulation process is executed, the amount of plasma in the plasma collection bag 25 gradually decreases until plasma begins to be introduced into the plasma collection bag 25 in the plasma collection process.

  In step S412, when the amount of plasma collected in the plasma collection bag 25 is equal to or less than the second amount, the rotation speed of the second liquid feeding pump 12 is changed to a high speed (step S416), that is, 2 is set to a high liquid supply speed, flag i = 1 is set (step S417), flag j = 1 is set (step S418), the process returns to step S406, and again, Step S406 and subsequent steps are executed.

  In step S407, when the amount of plasma collected in the plasma collection bag 25 is less than the first amount, the second liquid feeding pump 12 is stopped (step S408), and the flag i = 0 is set. (Step S409), the flag k = 0 is set (Step S410), the process returns to Step S406, and Steps S406 and after are executed again.

  Here, when the plasma collection step is executed, the plasma flows out from the blood storage space 146 of the centrifuge 20, and the amount of plasma in the plasma collection bag 25 gradually increases.

  In step S407, if the amount of plasma collected in the plasma collection bag 25 is greater than or equal to the first amount, it is possible to execute the plasma circulation step and determine whether or not flag k = 1. Determination is made (step S411). If flag k = 0, it is determined whether flag i = 1 (step S413). If flag i = 0, the second liquid feed pump 12 is turned on. Operate and rotate at high speed (step S416), that is, the liquid feed speed of the second liquid feed pump 12 is set to a high liquid feed speed, flag i = 1 is set (step S417), and flag j = 1 (Step S418), the process returns to step S406, and step S406 and subsequent steps are executed again. Then, in step S414, “YES” is determined, the process proceeds to step S415, and it is determined whether or not the processed blood volume is equal to or larger than the predetermined amount (step S415). (Step S416), the flag i = 1 is set (step S417), the flag j = 1 is set (step S418), the process returns to step S406, and again, Step S406 and subsequent steps are executed.

  In step S415, if the processed blood volume is equal to or larger than the predetermined amount, the rotation speed of the second liquid feed pump 12 is changed to a low speed (step S419), that is, the liquid feed of the second liquid feed pump 12 is changed. The speed is set to a low liquid feed speed, flag j = 0 is set (step S420), the process returns to step S406, and step S406 and subsequent steps are executed again.

  In step S406, when the interface B reaches the first position, the first liquid feeding pump 11 is stopped, the plasma collection process is terminated, and the plasma circulation process is executed alone (step S421). ).

  Next, a platelet collection process is executed (step S422), and a blood return process is executed (step S423).

As described above, when the plasma collection process is interrupted in the middle, the plasma circulation process having the pattern shown by the broken line in FIG. 9 can be executed. When the plasma collection process is not interrupted, the plasma at a low liquid feeding speed can be performed. A circulation step can be performed.
According to this blood component collection device 1, the same effects as those of the second embodiment described above can be obtained.

<Other embodiments>
FIGS. 11 to 13 are timing charts of other embodiments of the blood component collection device of the present invention, respectively, and FIGS. 11 to 13 show the operations in the respective cycles after the second cycle. Yes.

  The plasma circulation process in each cycle after the second cycle of the blood component collecting apparatus 1 may be as shown in FIGS.

  As mentioned above, although the blood component collection device of the present invention has been described based on the illustrated embodiment, the present invention is not limited to this, and the configuration of each part is an arbitrary configuration having the same function. Can be replaced. Moreover, other arbitrary structures and processes may be added to the present invention.

  Further, the present invention may be a combination of any two or more configurations (features) of the above embodiments.

  In addition, the blood component collection device of the present invention is not limited to the application to obtain both platelet preparations and plasma preparations (or the raw material plasma of plasma fractionation preparations), but only to obtain platelet preparations from blood. You may apply.

  Moreover, the blood component collection device of the present invention is not limited to the application to obtain a platelet preparation or a plasma preparation, and may be applied to obtain an erythrocyte preparation, a leukocyte preparation, etc. from blood, for example. That is, in the blood component collection device of the present invention, the blood cell component collected in the blood component collection bag is not limited to platelets (platelets including plasma), but, for example, red blood cells (red blood cells including plasma), white blood cells (white blood cells including plasma) Or the like.

  In the present invention, the blood separator is not limited to the centrifugal type, and may be, for example, a membrane type.

  In the present invention, the cells separated and removed by the cell separation filter (filter) are not limited to leukocytes.

  In the present invention, the optical sensor is not limited to the illustrated one, and may be a line sensor, for example.

  In the above-described embodiment, the case where the present invention is applied to a so-called intermittent type (also referred to as a single-needle type or one-arm type) blood component collecting apparatus in which a blood collection line and a blood return line are integrated has been described. The present invention is not limited to this type, but can be applied to, for example, a so-called continuous type (also referred to as a two-needle type or both-arm type) blood component collecting apparatus in which a blood collection line and a blood return line are separately provided it can.

  The present invention can also be applied to a blood component collection device that collects predetermined blood components and does not return the remaining blood components to the donor (no blood return step).

It is a top view which shows 1st Embodiment of the blood component collection apparatus of this invention. It is a partially broken sectional view of the state where the centrifuge was installed in the centrifuge drive device with which the blood component collection device shown in FIG. 1 is provided. It is a timing chart for demonstrating operation | movement of the blood component collection device shown in FIG. It is a timing chart for demonstrating operation | movement of the blood component collection device shown in FIG. It is a flowchart for demonstrating control operation of the control part of the blood component collection device shown in FIG. It is a flowchart for demonstrating control operation of the control part of the blood component collection device shown in FIG. It is a timing chart for demonstrating the operation | movement in 2nd Embodiment of the blood component collection device of this invention. It is a flowchart for demonstrating control operation of the control part in 2nd Embodiment of the blood component collection device of this invention. It is a timing chart for demonstrating the operation | movement in 3rd Embodiment of the blood component collection device of this invention. It is a flowchart for demonstrating control operation of the control part in 3rd Embodiment of the blood component collection device of this invention. It is a timing chart of other embodiments of the blood component collection device of the present invention. It is a timing chart of other embodiments of the blood component collection device of the present invention. It is a timing chart of other embodiments of the blood component collection device of the present invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Blood component collection apparatus 2 Blood component collection circuit 3 Control part 10 Centrifugal drive device 11 1st liquid feed pump 12 2nd liquid feed pump 13 3rd liquid feed pump 14 Turbidity sensor 15 Optical sensor 151 Throw Light unit 152 Light receiving unit 153 Reflector 16 Weight sensor 17 Display / operation unit 18 Storage unit 20 Centrifuge 21 First line 21a Blood collection needle side first line 21b Centrifuge side first line 21c Branch connector 21d Chamber 21f Branch Connector 21g First pump tube 21h Tube 21i Filter 22 Second line 22a Second pump tube 22b Branch connector 22c Branch connector 22d Branch connector 22e Branch connector 22f Filter 22g Branch connector 22h Filter 23 Third line 23 a third pump tube 23b sanitization filter 23c bubble removal chamber 23d anticoagulant container connection needle 24 plasma circulation line 25 plasma collection bag 26 platelet collection bag 261 leukocyte removal filter 27a intermediate bag 27b air bag 28 bag 29 blood collection Needle 31-36 Air bubble sensor 41-51 Tube 81-86 First to sixth flow path opening / closing means 131 Plasma layer 132 Buffy coat layer 133 Red blood cell layer 141 Tubular 142 Rotor 143 Inlet 144 Outlet 145 Upper 146 Blood storage space 147 Reflective surface 201 Housing 202 Leg 203 Motor 204 Rotating shaft 205 Fixing stand 206 Bolt 207 Spacer S101-S113 Step S201-S215 Step S301-S314 Step S401-S42 3 steps

Claims (14)

A blood collection means comprising a hollow needle for collecting blood from a donor,
A blood separator for separating blood collected by the blood collecting means;
A plasma collection bag for collecting plasma separated by the blood separator;
A blood component collection bag for collecting predetermined blood cell components separated by the blood separator;
A blood treatment line connecting the hollow needle and the inlet of the blood separator;
A blood component collection circuit comprising a plasma circulation line branched from a branch portion provided in the blood treatment line and connected to the plasma collection bag;
A first liquid delivery means installed in the blood treatment line and delivering at least a liquid in the blood treatment line;
A second liquid feeding means installed in the plasma circulation line and for feeding at least the plasma in the plasma collection bag;
A plasma collecting step of operating the first liquid feeding means, transferring the blood collected by the blood collecting means to the blood separator, and collecting the plasma separated by the blood separator in the plasma collection bag; The second liquid feeding means is operated to transfer the plasma collected in the plasma collection bag to the blood separator via the plasma circulation line, and between the plasma collection bag and the blood separator. The first blood circulation step for circulating the blood plasma and the blood component collection step for collecting the predetermined blood cell component in the blood component collection bag by operating the second liquid feeding means. Liquid feeding means and liquid feeding control means for controlling the operation of the second liquid feeding means,
The liquid supply control means operates the second liquid supply means without interrupting the plasma collection step while the plasma collection step is being performed by operating the first liquid supply unit. The blood circulation process is performed, and after the plasma collection process is completed, the blood component collection process is performed, and the processed blood volume reaches a predetermined amount during the plasma circulation process. The blood component collection is characterized by having a flow rate control function for changing the flow rate of plasma circulating between the plasma collection bag and the blood separator by changing the liquid supply speed of the second liquid supply means. apparatus.   When the amount of blood to be processed reaches a predetermined amount, the flow rate control function decreases the flow rate of the second liquid feeding unit to circulate between the plasma collection bag and the blood separator. The blood component collection device according to claim 1, wherein the blood component collection device is configured to reduce the amount of the blood component.   The liquid feeding control means includes at least one cycle of blood component collection operation including the plasma collection step, the plasma circulation step, the blood component collection step, and a blood return step of returning the remaining blood components to a blood donor. The blood component collection device according to claim 1 or 2, wherein the blood component collection device is executed.   The flow rate control function is configured to start the plasma circulation step when the amount of plasma collected in the plasma collection bag reaches a first amount capable of executing the plasma circulation step in the first cycle. The blood component collection device according to claim 3.   The liquid feeding control means starts the plasma collection step and the plasma circulation step substantially simultaneously in each cycle after the second cycle, and in the middle of executing the plasma circulation step, When the amount of plasma collected is less than a first amount that can perform the plasma circulation step, the plasma circulation step is interrupted, and the amount of plasma collected in the plasma collection bag reaches the first amount. Then, the plasma circulation process is restarted, the liquid supply speed of the second liquid supply means before the plasma circulation process is interrupted, and the second liquid supply means after the plasma circulation process is restarted. The blood component collection device according to claim 3 or 4, which has a flow rate control function for setting the fluid velocity to a value different from each other.   6. The blood component collection apparatus according to claim 5, wherein the flow rate control function is configured to increase a liquid feeding speed of the second liquid feeding means after restarting the plasma circulation process before it is interrupted. . Based on the blood donor's hematocrit value, whether or not the amount of plasma collected in the plasma collection bag is less than a first amount capable of performing the plasma circulation step during the execution of the plasma circulation step. Having a prediction means to predict
The liquid feeding control unit starts the plasma collection step and the plasma circulation step substantially simultaneously in each cycle after the second cycle, and the plasma is being performed while the plasma circulation step is being executed by the prediction unit. If the amount of plasma collected in the collection bag is predicted to be less than the first amount, the amount of plasma collected in the plasma collection bag during the execution of the plasma circulation step Is less than or equal to a second amount greater than the first amount, the liquid feeding speed of the second liquid feeding means is increased, and then the amount of plasma collected in the plasma collection bag becomes the first amount. The flow rate control function of interrupting the plasma circulation step when the amount is less than the amount, and resuming the plasma circulation step when the amount of plasma collected in the plasma collection bag reaches the first amount. Blood component collection according to 4 Apparatus.   The flow rate control function makes the liquid feeding speed of the second liquid feeding means after resuming the plasma circulation process smaller than the liquid feeding speed of the second liquid feeding means before interrupting the plasma circulation process. The blood component collection device according to claim 7, which is configured to do so.   When the amount of blood to be processed reaches a predetermined amount, the flow rate control function decreases the flow rate of the second liquid feeding unit to circulate between the plasma collection bag and the blood separator. The blood component collection device according to claim 7, wherein the blood component collection device is configured to reduce the amount of the blood component.   The flow rate control function includes: a liquid feeding speed of the second liquid feeding means before the plasma circulation process is interrupted; and a liquid feeding speed of the second liquid feeding means after the plasma circulation process is resumed. The blood component collection device according to claim 9, which is configured to be substantially the same. A blood collection means comprising a hollow needle for collecting blood from a donor,
A blood separator for separating blood collected by the blood collecting means;
A plasma collection bag for collecting plasma separated by the blood separator;
A blood component collection bag for collecting predetermined blood cell components separated by the blood separator;
A blood treatment line connecting the hollow needle and the inlet of the blood separator;
A blood component collection circuit comprising a plasma circulation line branched from a branch portion provided in the blood treatment line and connected to the plasma collection bag;
A first liquid delivery means installed in the blood treatment line and delivering at least a liquid in the blood treatment line;
A second liquid feeding means installed in the plasma circulation line and for feeding at least the plasma in the plasma collection bag;
A plasma collecting step of operating the first liquid feeding means, transferring the blood collected by the blood collecting means to the blood separator, and collecting the plasma separated by the blood separator in the plasma collection bag; The second liquid feeding means is operated to transfer the plasma collected in the plasma collection bag to the blood separator via the plasma circulation line, and between the plasma collection bag and the blood separator. The first blood circulation step for circulating the blood plasma and the blood component collection step for collecting the predetermined blood cell component in the blood component collection bag by operating the second liquid feeding means. Liquid feeding means and liquid feeding control means for controlling the operation of the second liquid feeding means,
The liquid feeding control means uses the liquid feeding amount of the first liquid feeding means as a reference when the first liquid feeding means and the second liquid feeding means are operating in time overlap. The blood has a flow rate control function for changing the flow rate of the plasma circulating between the plasma collection bag and the blood separator by changing the liquid feeding speed of the second liquid feeding means. Component collection device.   The flow rate control function operates the second liquid delivery means without interrupting the plasma collection process while the plasma collection process is being performed by operating the first liquid delivery means. The plasma circulation step is executed, and after the plasma collection step is completed, the blood component collection step is executed, and the plasma collected in the plasma collection bag is being processed while the plasma circulation step is being executed. When the amount becomes less than a predetermined amount, the plasma circulation step is interrupted. When the amount of plasma collected in the plasma collection bag reaches the predetermined amount, the plasma circulation step is resumed and the plasma circulation step is interrupted. The liquid feeding speed of the second liquid feeding means before and the liquid feeding speed of the second liquid feeding means after resuming the plasma circulation step are set to different values. Item 12. The blood component collection device according to Item 11. Based on the blood donor's hematocrit value, whether or not the amount of plasma collected in the plasma collection bag is less than a first amount capable of performing the plasma circulation step during the execution of the plasma circulation step. Having a prediction means to predict
The flow rate control function operates the second liquid delivery means without interrupting the plasma collection process while the plasma collection process is being performed by operating the first liquid delivery means. After the plasma circulation step is executed and the plasma collection step is completed, the blood component collection step is executed, and the plasma collecting step is collected in the plasma collection bag while the plasma circulation step is being executed by the prediction means. When the amount of plasma collected is predicted to be less than the first amount, the amount of plasma collected in the plasma collection bag during the execution of the plasma circulation step is less than the first amount. When the amount is less than or equal to a second amount greater than the amount, the liquid feeding speed of the second liquid feeding means is increased, and then the amount of plasma collected in the plasma collection bag is less than the first amount, The plasma circulation process is interrupted and collected in the plasma collection bag. Has been the amount of plasma reaching the first amount, the blood component collection apparatus according to resume claim 11 the plasma circulation step.   The blood component collecting apparatus according to claim 1, wherein the predetermined blood cell component is platelets.

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