JP2007237021A - Microreactor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microreactor in which a part of a reagent is not stayed in a storage chamber and a freedom of arrangement of the reagent storage part is further high when a drive liquid is poured to the storage chamber stored with the reagent and the reagent is pushed out to a downstream to feed the liquid. <P>SOLUTION: Length in the storage chamber 34a of the reagent storage part 33 is made 10 times or more relative to a flow passage width W and a shape of the storage chamber 34a is made to a shape in which at least a part is bent. Further, radius of curvature of a wall surface at a bent portion of the storage chamber 34a is made within a predetermined range. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a microreactor used for biological substance inspection / analysis by gene amplification reaction, antigen-antibody reaction, etc., inspection / analysis of other chemical substances, chemical synthesis of a target compound by organic synthesis, and the like.

In recent years, by making full use of micromachine technology and ultrafine processing technology, devices and means (for example, pumps, valves, flow paths, sensors, etc.) for performing conventional sample preparation, chemical analysis, chemical synthesis, etc. have been miniaturized. A system integrated on a chip has been developed (Patent Documents 1 and 2). This is also called μ-TAS (Micro total Analysis System), bioreactor, Lab-on-chips, biochip, and is used in medical examination / diagnosis field, environmental measurement field, agricultural production field. Application is expected. As can be seen in the current genetic testing, when complicated processes, skilled techniques, and equipment operations are required, automated, accelerated and simplified microanalysis systems are costly and require samples. The benefits of enabling analysis not only in volume and time but also in any time and place are enormous.

  In various types of analysis and inspection, importance is attached to the quantitativeness of analysis, the accuracy of analysis, and the economic efficiency of these analysis chips. For this purpose, it is a challenge to establish a highly reliable liquid delivery system with a simple configuration, and there is a need for a microfluidic control element that is highly accurate and excellent in reliability. The control method is proposed (patent documents 3 to 5).

As an analysis chip for feeding liquid by the micropump system as described above, the present applicant encloses a reagent in a flow path in the chip in advance, and pushes the enclosed reagent downstream with a driving liquid during analysis, Patent Document 1 proposes a microreactor configured to mix and react reagents with each other downstream.
Japanese Patent Application No. 2004-138959 JP 2004-28589 A JP 2001-322099 A JP 2004-108285 A JP 2004-270537 A

  In such a microreactor, there are fine flow paths such as a reagent storage unit for storing reagents, a reagent mixing unit for mixing reagents, a reaction unit, a detection unit provided as necessary, and a storage unit for various processing solutions. Since it is arranged in one chip, it often has a complicated flow path configuration. Therefore, it is required to arrange the flow paths efficiently.

However, when the shape of the reagent storage portion is a wide liquid reservoir shape as shown in FIGS. 14 to 17 of Patent Document 1, for example, the degree of freedom of the flow path arrangement is reduced.
In addition, the reagent container having such a shape has the following problems. FIG. 6 is an enlarged view of the liquid reservoir-shaped reagent container. In the reagent storage unit 133 in the figure, an inlet 134b through which the driving liquid from the driving liquid channel 51 is injected is provided on the upstream side of the storage chamber 134a in which the reagent 53 is stored, and on the downstream side of the storage chamber 134a. Is provided with an outlet 134c to the reagent delivery channel 52.

  When the driving liquid is injected from the driving liquid channel 51 into the storage chamber 134a by a micro pump (not shown), the reagent 53 is pushed downstream and sent out from the outlet 134c to the reagent delivery channel 52. At this time, since the reagent container 133 has a simple wide shape in which the distance between the injection port 134b and the outlet 133c is short, only the reagent at the center is pushed away, making it difficult for the reagent on the wall surface to flow. 157 will occur.

  In order to prevent such stagnation of the reagent, a method of making the reagent storage section a narrow flow path is conceivable. However, if the reagent container is a narrow linear channel, the distance between the inlet and the outlet in the reagent container is increased, and the channel longitudinal direction at the inlet and the channel length at the outlet are increased. The directions are in the same direction. Then, when a chip is accidentally dropped, a reagent leaks easily to the outside of the reagent container when a one-way force is applied to the reagent stored in the chip.

  Furthermore, when arranging a large number of reagent containing parts containing different reagents in a small chip, especially in a system in which they are intricately connected via a flow path, the reagent containing part is linear. The space inside the chip cannot be used up effectively, and as a result, the chip must be enlarged.

  As a method for solving such a problem, as shown in FIG. 7, it is conceivable to make the reagent container 133 into a shape obtained by bending a thick flow path. However, if the curvature radii of the outer wall 156 and the inner wall 155 in the bent portion are small with respect to the flow path width, a reagent retaining portion 157 is generated in the range including the vicinity of the edge portion of the outer wall, and the reagent It will interfere with the liquid transfer.

  The present invention prevents a part of the reagent from staying in the storage chamber when the driving liquid is injected into the storage chamber in which the reagent is stored and the reagent is pushed downstream to send the solution. An object of the present invention is to provide a microreactor having a high degree of freedom in arrangement. In particular, it is an object of the present invention to provide a microreactor capable of effectively preventing the reagent from staying in the bent portion even when the shape of the reagent containing portion is a shape in which the flow path is bent.

  It is another object of the present invention to provide a microreactor capable of efficiently arranging a plurality of reagent accommodating portions in a narrow space even when a plurality of reagent accommodating portions are arranged in a chip.

Another object of the present invention is to provide a microreactor capable of effectively suppressing the leakage of the reagent out of the reagent storage unit when the chip is stored or moved.
In addition, the present invention provides cooling even in the case where it is necessary to selectively cool a portion having a flow path containing a reagent in a chip by a cooling device using a Peltier element or the like as in gene amplification. An object of the present invention is to provide a microreactor having a small area and capable of efficiently cooling a reagent with a simple and compact apparatus configuration.

The microreactor of the present invention includes a reagent storage unit having a storage chamber in which a reagent is stored, an injection port for injecting a driving liquid into the storage chamber, and an outlet through which the reagent is pushed out of the storage chamber by the injected driving liquid. Is a microreactor that is provided in a plate-like chip and pushes the reagent from the outlet to the reagent delivery channel by injecting the driving liquid from the inlet into the storage chamber in which the reagent is stored,
The storage chamber of the reagent storage unit has a length that is at least 10 times the flow path width, and at least a part thereof is bent.

  The flow path width of the storage chamber is usually constant over the entire length of the flow path, but when there is a portion where the flow path width changes in a part of the storage chamber, the flow path width is constant and occupies most of the flow path longitudinal direction. The flow path width in a region having a flow path width of In addition, the cross-sectional shape of the flow path is usually a square or a rectangle, but the flow path width is the maximum width in a plane parallel to the chip surface.

  In this way, by making the shape of the storage chamber into a shape in which the flow path width is sufficiently narrow with respect to its length, the driving liquid is injected into the storage chamber in which the reagent is stored and the reagent is pushed downstream to send the solution. In doing so, it is possible to effectively prevent a part of the reagent from staying in the storage chamber.

  Furthermore, even if the storage chamber has a long shape as described above, it is possible to efficiently arrange the storage chamber in a limited region in the chip by making the storage chamber a shape in which the flow path is bent. The inlet and the outlet can be directed in any direction. That is, the freedom degree of arrangement | positioning of a reagent storage part becomes high.

  In the above invention, the curvature radius of the inner wall surface in the bent portion of the storage chamber is 1/5 times or more of the flow path width, and the curvature radius of the outer wall surface is 1/2 or more times of the flow path width. Preferably there is.

In this way, by setting the radius of curvature at the bent portion of the storage chamber within the above range, the retention of the reagent at the bent portion can be particularly effectively prevented.
In the above invention, it is preferable that an angle formed by a liquid feeding direction in at least a part of the flow path of the storage chamber and a liquid feeding direction in another part is 90 ° or more.

By doing in this way, even if it is a case where several reagent accommodating parts are arrange | positioned in a chip | tip, for example, these reagent accommodating parts can be arrange | positioned efficiently in a narrow space.
In the above invention, the storage chamber preferably has a meandering shape in which a plurality of flow paths are folded back through the bent portion.

  By doing in this way, a reagent storage part becomes a shape by which the long flow path was efficiently arrange | positioned in the outline of simple shapes, such as square shape and a rectangular shape, for example. Therefore, even when it is necessary to selectively cool the range by pressing a cooling device using a Peltier element or the like within a specific range including the reagent storage unit, the cooling unit has a simple shape and a small cooling area. Therefore, the reagent can be efficiently cooled with a simple and compact apparatus configuration.

In the above invention, it is preferable that the longitudinal direction of the flow path at the inlet of the reagent storage unit and the longitudinal direction of the flow path at the outlet are different from each other.
By doing in this way, even if a force in one direction is applied to the reagent stored in the chip, for example, when the chip is accidentally dropped, the flow path longitudinal direction at the inlet and the outlet Since the longitudinal direction of the flow path is in a different direction, the reagent is difficult to leak out of the reagent storage unit. That is, it is possible to effectively suppress the leakage of the reagent out of the reagent storage unit when the chip is stored or moved.

  The microreactor according to the present invention has a configuration in which a plurality of reagent storage units that store different reagents are provided, and the reagents from the plurality of reagent storage units are merged and mixed in a junction provided downstream thereof. It is suitable for things.

  In the microreactor of the present invention, when the driving liquid is injected into the storage chamber in which the reagent is stored and the reagent is pushed out and sent downstream, a part of the reagent does not stay in the storage chamber. The degree of freedom of arrangement of parts is high.

  Hereinafter, the present invention will be described with reference to the drawings. FIG. 1 is a view showing a reagent storage unit of a microreactor according to an embodiment of the present invention. The reagent storage section 33 in the figure is a flow path disposed upstream of the reaction section in a fine flow path provided in a plate-shaped chip made of resin, and includes a storage chamber 34a in which the reagent 53 is stored. In addition, an inlet 34b for injecting the driving liquid into the storage chamber 34a and an outlet 34c through which the reagent 53 is pushed out of the storage chamber 34a by the injected driving liquid are provided.

  A micropump (not shown) is communicated with an opening provided upstream of the driving liquid flow path 51 in the chip constituting the microreactor, and the driving liquid is sent into the chip by the micropump, and from the inlet 34 b through the driving liquid flow path 51. By injecting the driving liquid into the storage chamber 34a, the reagent 53 is pushed out from the outlet 34c to the reagent delivery channel 52. The extruded reagent 53 is, for example, joined and mixed with a reagent from another reagent storage unit provided in the chip, and then subjected to the reaction downstream thereof.

  In this embodiment, a water repellent valve is provided at each of the inlet 34b and the outlet 34c of the reagent container 33. This water repellent valve has the structure shown in FIG. The water repellent valve 45 shown in the figure includes a liquid feeding control passage 46 having a small diameter. The liquid feeding control passage 46 is a narrow flow passage whose cross-sectional area (cross-sectional area of a cross section perpendicular to the flow passage) is smaller than the cross-sectional areas of the upstream flow passage 47a and the downstream flow passage 47b.

  When the flow path wall is made of a hydrophobic material such as resin, the aqueous liquid 48 in contact with the liquid supply control passage 46 passes through the flow path 47b on the downstream side due to the difference in surface tension with the flow path wall. Is regulated.

  When the liquid 48 flows out to the downstream flow path 47b, a liquid supply pressure higher than a predetermined pressure is applied by a micropump, whereby the liquid 48 flows from the liquid supply control passage 46 to the downstream flow against the surface tension. Push to path 47b. After the liquid 48 flows out to the flow path 47b, the liquid 48 flows to the downstream flow path 47b without maintaining the liquid supply pressure required to push the tip of the liquid 48 to the downstream flow path 47b. . That is, the passage of the liquid from the liquid feed control passage 46 is blocked until the liquid feed pressure in the positive direction from the upstream side to the downstream side reaches a predetermined pressure, and the liquid feed pressure exceeding the predetermined pressure is applied. 48 passes through the liquid feed control passage 46.

  By the action of the water repellent valve, the reagent 53 is prevented from flowing out of the storage chamber 34a from the inlet 34b and the outlet 34c in the reagent storage unit 33 of FIG. In use, the reagent 53 is pushed out from the water repellent valve at the outlet 34 c by the pressure from the upstream by the driving liquid and flows out to the liquid feeding control passage 46.

  In order to make the liquid holding force by the water repellent valve sufficient, an oily liquid that contacts the reagent 53 at the interface on each of the inlet 34b side and the outlet 34c side in the storage chamber 34a and an aqueous solution that contacts this oily liquid at the interface You may make it accommodate a liquid in this order.

In the present embodiment, the storage chamber 34 a of the reagent storage unit 33 has an elongated shape that is 10 times or more the length of the flow path width W, and a plurality of flow paths are folded back through a plurality of curved portions 54. It has a meandering shape. With the curved portion 54 as a boundary, the liquid feeding direction is switched from d ₁ to d ₂ by approximately 180 °, and the entire contour is a compact shape having a substantially square shape. Further, the curvature radius of the inner wall surface 55 in the curved portion 54 is 1/5 times or more of the flow path width W, and the curvature radius of the outer wall surface 56 is set to be 1/2 or more times of the flow path width W.

  Thus, by making the shape of the storage chamber 34a into a shape in which the flow path width W is sufficiently narrow with respect to its length, the driving liquid is injected into the storage chamber 34a to push the reagent 53 downstream and send it. In this case, it is possible to effectively prevent a part of the reagent 53 from staying as shown in FIG.

Further, by setting the radius of curvature of the curved portion 54 of the storage chamber 34a within the above range, it is possible to effectively prevent a part of the reagent 53 from staying in the curved portion 54 as shown in FIG.
In addition, each said numerical range can be estimated as a reasonable range with which the said effect is acquired also by simulation of a fluid.

  Further, in the present embodiment, the curved portion 54 is provided and the accommodating chamber 34a is shaped so that the flow path is bent. Therefore, the accommodating chamber 34a can be efficiently arranged in a limited region in the chip, and the injection port Since 34b and the outlet 34c can be directed to arbitrary directions, the freedom degree of arrangement | positioning of the reagent storage part 33 is high. For example, in the case where a plurality of reagent containing portions 33 are arranged in the chip, at least a part of the flow paths in the containing chamber 33a can be arranged from the point that these reagent containing portions 33 can be efficiently arranged in a narrow space. It is preferable that the angle formed by the liquid feeding direction in and the liquid feeding direction in other parts is 90 ° or more.

  Furthermore, in the present embodiment, the storage chamber 34a has a meandering shape, and has a shape in which long channels are efficiently arranged within the outline of a simple shape of a substantially square shape, so that the reagent can be used like a microreactor used in a gene amplification reaction. Even when it is necessary to selectively cool the range by pressing a cooling device using a Peltier element or the like within a specific range including the accommodating portion 33, the cooling portion has a simple shape and its cooling area is small. The reagent 53 can be efficiently cooled with a simple and compact apparatus configuration.

In this embodiment, a passage longitudinal direction d ₃ in the inlet 34b, a passage longitudinal direction d ₄ at the outlet 34c and is different from each other, these directions are substantially orthogonal.
By doing in this way, even if a force in one direction is applied to the reagent stored in the chip, for example, when the chip is accidentally dropped, the flow path longitudinal direction at the inlet and the outlet Since the longitudinal direction of the flow path is in a different direction, the reagent is difficult to leak out of the reagent storage unit. That is, it is possible to effectively suppress the leakage of the reagent out of the reagent storage unit when the chip is stored or moved.

  FIG. 3 is a diagram showing a modification of the above embodiment. In addition, the same code | symbol is attached | subjected to the same component and the description is abbreviate | omitted. In this modification, the basic configuration is the same as that of the above embodiment, but the flow path is switched in the opposite direction via the two curved portions 54 in the portion where the direction of the meandering shape is switched. Even with such a flow channel shape, the same effect as in the above embodiment is exhibited, and as a result, the same effect can be obtained.

  FIG. 4 is a plan view showing another embodiment of the microreactor of the present invention. In the microreactor of this example, a sample is injected into a channel in the chip, and a reagent in the channel is reacted with an ICAN (Isothermal chimera primer initiated nucleic acid amplification) method to amplify a gene in the sample and detect the detection. Is what you do.

  In the microreactor 1 in the figure, three types of reagents are accommodated in each of the reagent accommodating portions 33a, 33b, and 33c. These reagent storage portions 33a, 33b, and 33c have a meandering shape as shown in the embodiment of FIG. 1, and are provided at both ends thereof (in the reagent storage portion 33a, the upstream inlet 34b and the downstream outlet 34c). 2 is provided with a water repellent valve having the structure shown in FIG. 2, and a reagent is sealed in a flow path between these water repellent valves.

In the present embodiment, the widths of the reagent storage portions 33a, 33b, and 33c are 500 μm, and the interval between adjacent flow paths that are folded back at the bent portions and the liquid feeding directions are opposite to each other is 500 μm. The radius of curvature at the curved portion is 250 μm for the inner wall surface and 750 μm for the outer wall surface. The total length of the reagent storage units 33a and 33c is 43 mm, and the total length of the reagent storage unit 33b is 17 mm.

  Although not described in detail, the water repellent valve of FIG. 2 is provided in the fine flow path of the microreactor 1 of FIG. 4 at positions other than both ends of the reagent storage units 33a to 33c. The water repellent valve is also provided at the mixed reagent inlet and the sample inlet at the mixed reagent and sample junction 38. These water repellent valves control the timing of liquid supply start to the flow path ahead.

  Openings 32c to 32e opened from one surface of the microreactor 1 to the outside are provided on the upstream side of the reagent containers 33a to 33c in FIG. These openings 32c to 32e are aligned with flow path openings provided on the connection surface of the micropump unit when the microreactor 1 is connected to a micropump unit to be described later and connected to the micropump.

  Similarly, the openings 32a, 32b, and 32f to 32k communicate with the micropump by connecting the microreactor 1 and the micropump unit. A pump connection portion is constituted by a chip surface including these openings 32a to 32k, and the microreactor 1 and the micropump unit are connected by bringing the pump connection portion into close contact with the connection surface of the micropump unit.

  Reagents stored in the reagent storage portions 33a to 33c are poured into the downstream end portions of the reagent storage portions 33a to 33c by flowing drive liquid from the openings 32c to 32e by separate micro pumps communicating with the openings 32c to 32e, respectively. It is pushed forward from the provided water repellent valve and flows into the merging portion 35, and each of the three types of reagents is mixed in the reagent mixing flow path 36 that follows.

  Thereafter, the mixed reagent merges with the sample accommodated in the sample receiving portion 37 at the merge portion 38. The mixed reagent is pushed downstream by the driving liquid by the micro pump communicated with the opening 32b, and the sample is pushed downstream by the driving liquid by the micro pump communicated with the opening 32a. The mixed solution of the mixed reagent and the sample is accommodated in the reaction unit 39, and the gene amplification reaction is started by heating.

  The liquid after the reaction is sent to the detection unit 40, and the target substance is detected by, for example, an optical detection method. Each of the reagents (for example, a solution for stopping the reaction between the mixed reagent and the sample, a substance to be detected) previously stored in the flow path ahead of these openings by separate micro pumps communicating with the openings 32f to 32j. On the other hand, a liquid for performing necessary processing such as a label, a cleaning liquid, and the like) are extruded downstream at a predetermined timing and fed.

  FIG. 5 is a perspective view showing a part of a micropump unit used in the microreactor of FIG. The micropump unit 11 includes a silicon substrate 17 and anodic bonded glass substrates 18 and 19.

  The micro pump 12 is constituted by the internal space between the substrate 17 and the substrate 18. The micro pump 12 is a bidirectional drive pump having a structure disclosed in Japanese Patent Application Laid-Open No. 2001-322099 using a piezo actuator.

  A flow path 20 is patterned on the substrate 19, and on the downstream side of the flow path 20, the micro pump 12 is communicated with the micro flow path of the chip by being aligned with the openings 32a to 32k of the microreactor 1 of FIG. An opening 15 is provided for this purpose.

  The upstream side of the flow path 20 communicates with the micro pump 12 through the flow path provided in the substrate 17 via the through hole 16 b of the substrate 18. The upstream side of the micropump 12 communicates with the opening 14 provided in the substrate 19 from the flow path provided in the substrate 17 through the through hole 16 a of the substrate 18. The opening 14 is connected to a driving liquid tank (not shown) via a packing.

  The openings 15a, 15b, and 15c communicate with the openings 32c, 32d, and 32e of the microreactor 1 shown in FIG. The micropump 12 sends the driving liquid through the flow path 20, the opening 15a, and the opening 32c, pushes the reagent stored in the reagent storage section 33a downstream, and sends the driving liquid through the flow path 20, the opening 15b, and the opening 32d. The reagent stored in the reagent storage unit 33b is pushed downstream, the driving liquid is fed through the flow path 20, the opening 15c, and the opening 32e to push the reagent stored in the reagent storage unit 33c downstream.

  A series of analysis steps of reaction and detection is performed in a state where the microreactor 1 of FIG. 4 is mounted on a system body in which a micropump, a temperature control device, a detection device, and the like are integrated. That is, sample and reagent feeding, various treatments before and after the amplification reaction, predetermined reaction based on mixing of the solution and optical measurement are automatically performed as a series of continuous steps, and the measurement data is necessary. It is stored as data along with conditions and recorded items.

  The regions of the reagent storage portions 33a to 33c in the microreactor 1 are selectively cooled by pressing a Peltier element installed in the system main body from the back surface of the chip, thereby preventing reagent alteration and the like. On the other hand, the region of the reaction part 39 is selectively heated by pressing a heater from the back surface of the chip, thereby bringing the reaction part 39 to a temperature suitable for the reaction.

  At this time, in the embodiment of FIG. 4, the reagent storage portions 33a to 33c are meandering and the entire outline is a simple and compact shape of a substantially square or substantially rectangular shape, so that the cooling region of the chip is also a simple shape, Furthermore, since the cooling area is reduced, the reagent can be efficiently cooled with a simple and compact apparatus configuration.

  The present invention has been described above based on the embodiments. However, the present invention is not limited to these embodiments, and various modifications and changes can be made without departing from the scope of the present invention.

FIG. 1 is a view showing a reagent storage unit of a microreactor according to an embodiment of the present invention. FIG. 2 is a view showing a water repellent valve. FIG. 3 is a view showing a modification of the reagent container in the embodiment of FIG. FIG. 4 is a plan view showing another embodiment of the microreactor of the present invention. FIG. 5 is a perspective view showing a part of a micropump unit used in the microreactor of FIG. FIG. 6 is a diagram showing an example of the reagent storage unit of the microreactor. FIG. 7 is a diagram illustrating an example of a reagent storage unit of the microreactor.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Microreactor 11 Micropump unit 12 Micropump 13 Chip connection part 14 Opening 15 and 15a-15c Opening 16a Through-hole 16b Through-hole 17 Substrate 18 Substrate 19 Substrate 20 Channel 32a-32k Opening 33, 33a-33c Reagent storage part 34a accommodation Chamber 34b Inlet 34c Outlet 35 Junction part 36 Reagent mixing channel 37 Sample receiving part 38 Junction part 39 Reaction part 40 Detection part 45 Water repellent valve 46 Liquid feed control path 47a Upstream path 47b Downstream path 48 Liquid 51 driving liquid channel 52 reagent delivery channel 53 reagent 54 curved portion 55 inner wall 56 outer wall W channel width d ₁ reagent traveling direction d ₂ reagent traveling direction d ₃ driving fluid injection direction d ₄ reagent pushing direction

Claims (6)

A reagent storage unit having a storage chamber in which a reagent is stored, an injection port for injecting the driving liquid into the storage chamber, and an outlet through which the reagent is pushed out of the storage chamber by the injected driving liquid is formed in the plate-shaped chip. A microreactor that pushes a reagent from the outlet to a downstream channel by injecting a driving liquid from the inlet into a storage chamber in which the reagent is stored,
The microreactor is characterized in that the storage chamber of the reagent storage section has a length of 10 times or more with respect to the flow path width, and at least a part thereof is bent.   The curvature radius of the inner wall surface in the bent portion of the storage chamber is 1/5 times or more of the flow path width, and the curvature radius of the outer wall surface is 1/2 or more times of the flow path width. The microreactor according to claim 1.   The microreactor according to claim 1 or 2, wherein an angle formed by a liquid feeding direction in at least a part of the flow path of the storage chamber and a liquid feeding direction in another part is 90 ° or more.   The microreactor according to any one of claims 1 to 3, wherein the storage chamber has a meandering shape in which a plurality of flow paths are folded back through the bent portion.   The microreactor according to any one of claims 1 to 4, wherein a flow path longitudinal direction at the injection port of the reagent container and a flow path longitudinal direction at the outlet are different from each other.   6. A plurality of reagent storage portions for storing different reagents are provided, and a merging portion for merging reagents from the plurality of reagent storage portions is provided downstream thereof. A microreactor according to any one of the above.

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