JP2005289999A - Cosmetic composition containing extract of zizyphus jujuba and extract of juglans sinensis - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic composition synergistically exhibiting improved effects for promoting differentiation of cuticle, and effects for keeping moist of the skin. <P>SOLUTION: The cosmetic composition contains both of an extract of Zizyphus jujuba and an extract of Juglans sinensis. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention includes a jujube extract and a walnut extract, and particularly relates to a cosmetic composition excellent in keratin differentiation promoting effect and skin moisturizing effect.

  Skin (skin), which plays an important role as a barrier that protects the living body from the external environment, forms a thin stratum corneum containing moisture through differentiation processes in the epidermis layer in order to prevent moisture loss. . This stratum corneum is located in the outermost stratum corneum of the skin that is in direct contact with the external environment, prevents the entry of physically and chemically harmful components into the living body, and is a dry environment from the living body. Even underneath, it prevents water loss in the body, and the stratum corneum itself retains flexibility by holding moderate water. The moisture content of the skin is about 70% in the dermis, but the moisture content decreases as it moves to the epidermis side, and is about 10% to about 30% in the stratum corneum of the outermost horny layer. When moderate moisture is retained in the stratum corneum, the elasticity and flexibility of the skin is maintained, and generally normal skin contains about 30% moisture and water-soluble components. Yes. In addition, high concentrations of natural moisturizing factors (NMF) are present in the horny cells of healthy people, which assists the moisture retention of the skin.

  Despite this function, the skin is exposed to the harsh external environment, i.e. wind and rain, cold weather, sunlight, etc., as well as facial cleansing, shaving, winter indoor heating, and contaminated atmosphere. In addition, the skin moisture is easily lost due to factors such as aging as aging progresses, and the skin becomes easy to dry, so there is a long-awaited skin moisturizer that prevents this phenomenon. Yes.

  There are many substances that show moisturizing effects, and research to deal with such phenomena has been actively conducted, so far, it has been used for skin that can increase the amount of moisture in the stratum corneum. Known moisturizer components include lipid components such as ceramide, essential fatty acids and lipid complexes (see Non-Patent Document 1).

Recently, not only simply increasing the amount of water in the stratum corneum, but also when the differentiation process in which the keratinocytes of the basal layer develop into the stratum corneum of the outermost stratum corneum functions normally. The fact that it acts as a moisture barrier has been newly elucidated. In other words, from a physiological viewpoint, dry skin is a phenomenon in which the amount of moisturizing factor and lipid in the skin stratum corneum decreases due to longer detachment time of the stratum corneum and decreased lipid synthesis ability of the epidermis cells. Therefore, the function of maintaining the moisture content of the skin can be enhanced by promoting the differentiation of keratinocytes. Therefore, it is expected that an improved skin moisturizing effect can be obtained by using a moisture retention substance in the stratum corneum together with a substance that plays a role in promoting keratin differentiation.
J. Invest. Dermatol., 5, pp.731-740 (1994)

  Coupled with the recent increase in interest in Kampo, active research is being conducted to obtain useful ingredients for the human body from natural herbal medicines that are safe for the human body. A cosmetic composition that synergistically exhibits an accelerating effect and a skin moisturizing effect has not yet been realized.

  In view of the technical problems recognized in the above-mentioned prior art, the inventors of the present application have studied earnestly herbal medicine ingredients having a skin moisturizing effect, and the ingredients that promote skin keratin differentiation in the jujube extract, Moreover, it came to discover that the ingredient which supplies a lipid is contained in the walnut extract. That is, the gist of the present invention resides in a cosmetic composition containing a jujube extract and a walnut extract.

  According to the present invention, the intended purpose, which is to promote the differentiation of the cellular stratum corneum of human skin and to restore the barrier function of damaged skin, improve and maintain the moisturizing power of human skin. A cosmetic composition that exhibits the effect synergistically, that is, a cosmetic composition that can be suitably used in the application of a moisturizing agent for skin is realized.

  The configuration of the present invention will be described in detail below.

  The jujube extract used in the cosmetic composition of the present invention is used as a substance that promotes keratin differentiation of the skin, and the walnut extract contains abundant lipids and imparts moisture to the stratum corneum.

  Jujube and walnut extracts are obtained from jujube and walnut nuts, respectively.

  The cosmetic composition of the present invention preferably contains a jujube extract at a concentration of about 0.001% to about 20% by weight. This means that if the content of jujube extract is less than about 0.001% by weight, the desired skin moisturizing effect cannot be obtained, and if it exceeds about 20% by weight, the safety of the skin will be improved. This may cause an impact.

  The cosmetic composition of the present invention preferably contains a walnut extract at a concentration of about 0.001 wt% to about 20 wt%. This means that if the content of the walnut extract is less than about 0.001% by weight, the desired skin moisturizing effect cannot be obtained, and if it exceeds about 20% by weight, the safety of the skin will be improved. This may cause an impact.

  The composition ratio of the jujube extract and the walnut extract in the cosmetic composition of the present invention is adjusted to about 1: about 9 to about 9: about 1. This means that if either the jujube extract or the walnut extract in the cosmetic composition is too small compared to the other, it will promote the skin moisturizing action of promoting the differentiation of keratinocytes and adding moisture to the stratum corneum. This is because the two actions involved cannot be acquired synergistically. Therefore, the composition ratio of jujube extract and walnut extract needs to be adjusted to a composition ratio of at least about 1: about 9 to about 9: about 1.

  As used herein, the term “jujube” refers to the fruit of a Zizyphus jujuba and is also referred to as persimmon or honey. This real surface is rust-colored and oval, grows to a length of 1.5-2.5 cm, and becomes sweet when ripened red. In juicy dates, dry matter accounts for 20% -49%, 14-42% of which is composed of sugars such as fructose, sucrose, glucose, rhamnose. Organic acids such as malic acid, glucose, succinic acid, and glycolic acid account for 0.54% to 3.4%, and minerals such as potassium, calcium, and magnesium are also abundant. The fruit of the jujube is used not only as raw food but also as an ingredient for processing jujube sake, jujube tea, jujube vinegar, jujube candy, etc. in ordinary households. In the Kampo field, it is used as a crude drug for diuretics, tonics, and relaxation agents. In the field of Kampo, it is often used for diseases such as muscle tightness, muscle tightening and pain, coughing, pain in the whole body, etc. In addition to anti-allergic effects, It is known to be effective for exfoliation.

As used herein, the term “walnut” refers to the fruit of a walnut (Juglans sinensis), a deciduous tree of the walnut family, which is generally round and drupe. Europe is the origin, but it is also distributed in China and Asia and is cultivated all over the world. In South Korea, it is distributed mainly in the central and southern areas. As for the chemical component of walnuts, protein content is higher than meat, lipids are twice as much as pork, lipids are rich in unsaturated fatty acids, especially essential fatty acids that lower serum cholesterol. Essential fatty acids are known to prevent excessive adherence of cholesterol to blood vessels. In addition, if a large amount of minerals and vitamin B ₁ are ingested daily, the skin will become glossy, and anti-aging and tonic effects can be expected. According to the Herbs, walnuts are said to heal the liver, warm the hips and knees, and cure constipation. In addition to eliminating wrinkles and strengthening kidney function, it is also used in the treatment of nervous breakdown, such as enhancing memory. It is known to increase the water content of the stratum corneum using lipid components and the like, so walnuts rich in lipids are also thought to prevent skin dryness and improve skin moisture.

  The jujube extract and walnut extract of the present invention can be obtained by the following method.

  First, a jujube extract is extracted by adding a certain amount of water to the jujube and then boiling it for about 30 minutes to about 12 hours with a reflux apparatus. After filtering the extract and removing the supernatant, water is added to adjust the total amount. The jujube extract can be obtained by leaving the total amount of the adjusted aqueous solution at low temperature for 1 to 12 days and applying it to membrane filtration to remove precipitates and the like.

  On the other hand, the walnut extract is extracted by adding a certain amount of water to the walnut and then boiling for about 30 minutes to about 12 hours with a reflux apparatus. The extract is filtered through a paper filter, the supernatant is removed, and water is added to adjust the total amount. The walnut extract can be obtained by leaving the adjusted aqueous solution at low temperature for 1 to 12 days and applying it to membrane filtration to remove precipitates and the like.

  Next, each of the obtained jujube extract and walnut extract may be mixed at a ratio of about 1: about 9 to about 9: about 1 to obtain a mixture of jujube extract and walnut extract. it can.

  There is also provided a cosmetic composition comprising the jujube extract and the walnut extract thus obtained, and in particular, a cosmetic composition that can be suitably used for a skin moisturizer.

  The cosmetic composition of the present invention may be further added with other moisturizing ingredients that assist or improve these functions and effects as long as the effects of the present invention are not substantially impaired, in addition to the aforementioned extract. it can. Such components include, but are not limited to, ceramide, glycerol, glycerin and the like.

  The jujube extract and the walnut extract of the present invention can be applied as they are in the form of a spring, a spring, a spring, or a dry powder of the spring, or in the form of a normal external preparation or cosmetic. It can also be.

  The cosmetic composition of the present invention containing a jujube extract and a walnut extract can be used in various products such as cosmetics, facial cleansers and shampoos for the skin moisturizing effect. The products to which the cosmetic composition of the present invention can be added include cosmetics such as various creams, milk lotions and skin lotions, as well as shampoos, rinses, cleansings, facial cleansers, soaps, treatments and serums. It is not limited.

  Moreover, the cosmetic composition of the present invention containing a jujube extract and a walnut extract can be in the form of a solution, an emulsion, a viscous mixture, or the like. Examples of products for which the cosmetic composition of the present invention can be used include emulsions, creams, lotions, essences, packs, gels, powders, lipsticks, makeup bases, foundations, lotions, ointments, gels, patches, serums, Cleansing foams, cleansing creams, cleansing waters, soaps, sprays and the like are not limited to these.

  It is easy for those skilled in the art to select and incorporate ingredients other than jujube extract and walnut extract into a product using the cosmetic composition of the present invention according to the cosmetic dosage form or purpose of use. It can be done.

  For the purpose of enhancing the moisturizing effect, the cosmetic composition of the present invention can be used in combination with a substance that promotes absorption into the skin.

  In addition, water-soluble vitamins, oily vitamins, polymer peptides, polymer polysaccharides, sphingolipids, seaweed extracts and the like can also be added to the cosmetic composition of the present invention.

  Furthermore, in addition to the above-described components, the cosmetic composition of the present invention can be used in combination with other optional components blended in a normal cosmetic as necessary. As such optional ingredients, oil and fat ingredients, moisturizers, emollients, surfactants, organic pigments, inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjustment Examples include, but are not limited to, agents, alcohols, pigments, fragrances, blood circulation promoters, cooling agents, antiperspirants, and purified water. The blending amount of these optional components should be an amount that does not substantially impair the effects achieved by the present invention, and is preferably about 0.01% by weight to about 5% by weight of the cosmetic composition of the present invention, preferably , Adjusted to an amount of about 0.01 wt% to about 3 wt%.

  The present invention will be described below with reference to examples thereof, but it is needless to say that the present invention should not be construed in a limited manner based on the disclosure of the examples.

Example 1: Production of jujube extract
After adding 100 ml of water to 10 g of jujube, extraction was performed by boiling for 3 hours with a reflux apparatus. The extract is filtered through a paper filter, the supernatant is removed, and water is added to adjust the total volume to about 100 ml. The total amount of the aqueous solution was allowed to stand at 4 ° C. for 6 days, and then applied to 0.45 μm membrane filtration to remove precipitates and the like to obtain a jujube extract.

Example 2: Production of walnut extract
After adding 100 ml of water to 10 g of walnuts, extraction was performed by boiling for 3 hours with a reflux apparatus. The extract is filtered through a paper filter, the supernatant is removed, and water is added to adjust the total volume to about 100 ml. The total amount of the aqueous solution was allowed to stand at 4 ° C. for 6 days, and then applied to 0.45 μm membrane filtration to remove precipitates and the like, thereby obtaining a walnut extract.

Example 3: Production of a mixture of jujube extract and walnut extract The jujube extract obtained in Example 1 and the walnut extract obtained in Example 2 were each in a ratio of 1: 2, that is, 100 g of jujube extract. And 200 g of walnut extract were mixed to produce a mixture (300 g) of jujube extract and walnut extract.

Experimental Example 1: Differentiation-inducing effect of jujube extract and walnut extract in keratinocytes In order to verify the differentiation-inducing effect in keratinocytes produced by jujube extract and walnut extract, the following procedure was performed. It was.

  First, human keratinocytes at a density of 5,000 cells / well are placed in a 96-well microtiter plate containing keratinocyte growth (KGM) medium containing 1.0% fetal bovine serum. Added as follows. After the cells were attached to the wells, the old KGM medium was replaced with a fresh KGM medium not containing fetal calf serum, and the cells were cultured until the growth reached about 70% to about 80%. The cultured human keratinocytes were transferred to a culture flask, where they were cultured until the degree of growth reached about 80%.

  The culture was then incubated for 4 days with 200 ml each of the jujube extract obtained in Example 1, the walnut extract obtained in Example 2, or the extract mixture obtained in Example 3. Reducing agents such as urea, sodium dodecyl sulfate (SDS), and beta-mercaptoethanol were mixed to remove contaminating proteins.

  The remaining stratum corneum was subjected to absorbance measurement at 310 nm, and its peptide concentration was measured. A group treated with low concentration calcium (0.05 mM) without using any extract was defined as a negative control group. In addition, a group treated with high concentration calcium (1.5 mM) without using any extract was defined as a positive control group.

  The measurement results are shown in Table 1 below.

  As is clear from the results shown in Table 1, the proliferation effect of the keratinocytes was 31% for the walnut extract and 57% for the jujube extract, whereas the mixture of the jujube extract and the walnut extract was 72%. That is, the effect of inducing keratin differentiation of the jujube extract was superior to that of the walnut extract, but it was found to be far from the effect of inducing keratin differentiation in the mixture of jujube extract and walnut extract.

Experimental Example 2: Barrier recovery effect on animal skin by jujube extract and walnut extract In order to verify the barrier recovery effect of animal skin produced by jujube extract and walnut extract, an experiment was performed according to the following procedure. . That is, in this experimental example, the skin barrier recovery effect was measured based on changes in epidermal water loss (TEWL), also referred to as transepidermal water loss (TEWL), with the damaged barrier state being 100. did. This epidermal water loss refers to a phenomenon in which water supplied from the dermis of the skin is diffused to the upper part of the stratum corneum mainly by passive diffusion and discharged to the outside.

First, acetone was repeatedly applied to female hairless mice of 8 to 10 weeks old (source: Charles River) to damage the barrier function, and then a water loss measuring machine (evaporimeter EP1,
ServoMED) was used to measure epidermal water loss. As soon as the skin state becomes 4.0 g / m ² / h, samples containing each extract (jujube extract of Example 1, walnut extract of Example 2 or extract mixture of Example 3) are placed on the epidermis. Apply to 5cm ^{2 area} and measure epidermal water loss, then measure water loss after 1 hour, 2 hours, 4 hours and 8 hours to evaluate the degree of recovery of skin barrier function did. In addition, the group which does not contain an extract was made into the negative control group, and the group using the well-known lipid mixture (Ceramide: cholesterol: fatty acid = 2: 1: 1) was made into the positive control group. As described above, the skin barrier recovery effect was measured based on the change in the amount of epidermal water loss (TEWL) with the damaged barrier state being 100.

  The measurement results are shown in Table 2 below.

  As is clear from the results shown in Table 2, the mixture of jujube extract and walnut extract showed almost the same recovery effect as the positive control group. In addition, when treated with jujube extract alone, the skin barrier recovery effect was relatively smaller than when treated with walnut extract alone. Therefore, it was found that the skin barrier effect is significantly improved when the jujube extract and the walnut extract are used in combination.

Experimental Example 3: Human skin moisturizing power improvement effect by jujube extract and walnut extract With respect to jujube extract and walnut extract, in order to verify the moisturizing power improving effect on human skin, the effect of increasing the moisture content of the skin The following procedure was carried out, and the subjects were asked to hear their opinions on improving the dryness of the skin.

Experimental Example 3-1-1: Preparation of Cosmetics Cosmetic ingredients (raw materials) described in Table 3 below were prepared. Then, the milk phase component and the aqueous phase component were completely dissolved at 70 ° C., and then emulsification was allowed to proceed for 5 minutes at 7,000 rpm to prepare creamy cosmetics A to E.

Experimental example 3-1-2: Effect of increasing the amount of moisture in skin For 140 women aged 20 to 40 years whose skin condition is not so good, twice a day for 1 month, creamy cosmetic A E was applied to the face and front of the body. Prior to the first application, under a constant temperature / humidity condition (24 ° C, humidity 40%), a moisture meter (corneometer, CM820 courage Khazaka electronic GmbH, Germany) measures the dryness of the skin. The measured value was used as the basic value. Then, after 1 week, 2 weeks, and 3 weeks, the moisture content of the skin was measured in the same manner, and the effect of increasing the moisture content was objectively evaluated from the obtained measured values. In addition, the group which does not contain an extract was made into the negative control group, and the group using the well-known lipid mixture (Ceramide: cholesterol: fatty acid = 2: 1: 1) was made into the positive control group.

Experimental Example 3-1-3: Test results The moisture increase rate of the skin after 1 week, 2 weeks and 3 weeks after starting the test is shown in Table 4 below.

  As is clear from the results shown in Table 4, it was confirmed that the moisture content of the skin was increased in the test group using the jujube extract, the walnut extract, and the mixture of the jujube extract and the walnut extract. Of these, the effect of increasing the amount of water produced by the mixture of jujube extract and walnut extract was particularly remarkable.

Experimental Example 3-2: Improvement of skin dryness The 140 female subjects mentioned above were interviewed about the dryness of the skin after the test period. The survey results are shown in Table 5 below.

  As is clear from the results described in Table 5, in the subjective evaluation of the subjects, the mixture of jujube extract and walnut extract left a moist feeling on the skin and did not give the subject an unpleasant dry feeling. . Therefore, it was confirmed that the skin moisturizing effect was improved by a mixture of jujube extract as a keratin differentiation promoter and walnut extract as a moisture-imparting substance.

Example 4: Use form of cosmetic composition Various preparations containing the cosmetic composition of the present invention were prepared. In addition, the unit of the component compounding amount in a present Example is displayed by weight%, unless there is particular notice.

1: Soap Soap was prepared using a material according to the blending ratio shown in Table 6 below.

2: Milk lotion A milk lotion was prepared using materials according to the blending ratio described in Table 7 below.

3: Cream A cosmetic cream was prepared using materials according to the blending ratio described in Table 8 below.

4: Pack A pack was prepared using materials according to the blending ratio described in Table 9 below.

5: Cosmetic liquid A cosmetic liquid was prepared using materials according to the blending ratios shown in Table 10 below.

The cosmetic composition of the present invention is useful as an active ingredient of a skin moisturizer that synergistically exhibits an improved keratin differentiation promoting effect and a skin moisturizing effect.

Claims (6)

  A cosmetic composition comprising a jujube extract and a walnut extract.   The cosmetic composition according to claim 1, wherein the cosmetic composition is a skin moisturizer comprising a jujube extract that is a keratin differentiation promoting component and a walnut extract that is a horny moisturizing component.   The cosmetic composition according to claim 1 or 2, wherein 0.001 to 20% by weight of the extract is the jujube extract.   The cosmetic composition according to claim 1 or 2, wherein 0.001 to 20% by weight thereof is the walnut extract.   The cosmetic composition according to any one of claims 1 to 4, wherein a composition ratio of the jujube extract and the walnut extract is 1: 9 to 9: 1. Consists of emulsion, cream, lotion, essence, pack, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, patch, serum, cleansing foam, cleansing cream, cleansing water, soap, spray The cosmetic composition according to any one of claims 1 to 5, which is in a form selected from a group.