JP2005106784A - Measuring cell of colorimeter or like, and use method thereof - Google Patents
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- 238000000034 method Methods 0.000 title claims description 16
- 238000005259 measurement Methods 0.000 claims abstract description 131
- 238000006243 chemical reaction Methods 0.000 claims abstract description 32
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 31
- 239000011347 resin Substances 0.000 claims abstract description 7
- 229920005989 resin Polymers 0.000 claims abstract description 7
- 239000012085 test solution Substances 0.000 claims description 30
- 239000007788 liquid Substances 0.000 abstract description 23
- 238000012360 testing method Methods 0.000 description 21
- 230000001678 irradiating effect Effects 0.000 description 7
- 238000005192 partition Methods 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
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- 238000004891 communication Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
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- 238000002834 transmittance Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
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Abstract
Description
本発明は、比色計等の測定セルおよびその使用方法に関するものである。 The present invention relates to a measurement cell such as a colorimeter and a method for using the same.
比色計や色濁計や分光光度計およびヘモクロビンメータ等の測定装置にあっては、検体と反応試薬を混合反応させた被検液を、透明な材料で形成した測定セル内に収容し、この測定セルを測定装置に装着する。そして、測定セルに収容された被検液に、光を照射して適宜にその光を透過や反射または屈折させて、被検液の吸光度や透過率や色の濃度などが測定される。 In measuring devices such as a colorimeter, turbidimeter, spectrophotometer, and hemoglobin meter, a test solution obtained by mixing and reacting a sample and a reaction reagent is contained in a measurement cell formed of a transparent material, The measurement cell is attached to the measurement device. Then, the test solution contained in the measurement cell is irradiated with light, and the light is appropriately transmitted, reflected, or refracted, and the absorbance, transmittance, color density, etc. of the test solution are measured.
この測定セルに収容させる被検液は、キャピラリーで採取した検体と反応試薬とを試験管内等でよく混合反応させ、キャピラリーを除いてこの混合液のみを被検液として測定セルの容器内に移し替えていた。 The test solution to be stored in the measurement cell is prepared by thoroughly mixing and reacting the sample collected by the capillary and the reaction reagent in a test tube or the like, and transferring only this mixed solution as the test solution into the container of the measurement cell except for the capillary. I was changing.
そこで、検体と反応試薬を試験管内などで混合反応させて被検液とし、この被検液を測定セルの容器内に移し替える手間が煩雑であるとともに、移し替える際に被検液を誤って零してしまう虞があった。しかも、検体が感染性のある血液等であるならば、被検液を零さぬように極めて注意深く作業しなければならない。 Therefore, the sample and reaction reagent are mixed and reacted in a test tube to make a test solution, and the trouble of transferring this test solution into the container of the measurement cell is troublesome. There was a risk of spilling. Moreover, if the specimen is infectious blood or the like, it must be worked very carefully so as not to spill the test liquid.
この被検液を測定セルの容器内に移し替える手間を省くために、発明者は、反応試薬が収容された測定セルの容器内に検体を採取したキャピラリーを挿入して容器を密閉し、測定セル内で検体と反応試薬を混合反応させて被検液とすることに想到した。 In order to save the trouble of transferring this test solution into the container of the measurement cell, the inventor inserted a capillary that collected the sample into the container of the measurement cell containing the reaction reagent, sealed the container, and measured. It was conceived that the sample and reaction reagent were mixed and reacted in the cell to obtain a test solution.
しかるに、測定セルの容器内で反応試薬と検体を混合して被検液とすることは、測定セルの容器からキャピラリーを取り出さなければならない。それは、図９に示すごとく、測定セル１０の測定セル容器１２の底部近くに比色計等で光を照射させて被検液を測定する場合に、キャピラリー１４が光の透過の邪魔となって正確に測定できない虞がある。そして、採取する検体の量が僅かであると、反応試薬と混合して作れる被検液１６も少量となることから、より確実に被検液１６を測定するために、従来の比色計等では測定セル容器１２の底部近くに光を照射することが一般的であった。なお、図９において、１８は、測定セル容器１２の上部の開口部を閉塞する蓋体であり、２０は被検液１６に光を照射する光源であり、２２は光源２０から発する光から所定の波長の光を抽出する光源フィルターであり、２４は被検液１６を透過した光を受光する受光体である。 However, mixing the reaction reagent and the sample in the container of the measurement cell to obtain a test solution requires taking out the capillary from the container of the measurement cell. As shown in FIG. 9, when the test liquid is measured by irradiating light near the bottom of the measurement cell container 12 of the measurement cell 10 with a colorimeter or the like, the capillary 14 obstructs light transmission. There is a possibility that it cannot be measured accurately. If the amount of the sample to be collected is small, the amount of the test liquid 16 that can be mixed with the reaction reagent is also small. Therefore, in order to measure the test liquid 16 more reliably, a conventional colorimeter or the like Then, it was common to irradiate light near the bottom of the measurement cell container 12. In FIG. 9, 18 is a lid that closes the upper opening of the measurement cell container 12, 20 is a light source that irradiates light to the test solution 16, and 22 is a predetermined light from the light emitted from the light source 20. A light source filter for extracting light having a wavelength of 24, and 24 is a light receiver for receiving light transmitted through the test liquid 16.
発明者は、測定セル容器１２内からキャピラリー１４を取り出さなくても測定の邪魔とならないような技術を特願２００２−２４１６５８号にて提案した。これは、測定セル容器１２内を縦方向の仕切板で２つに区分し、しかも被検液１６は測定セル容器１２内の底部で２つの区分間を移動自在とし、一方の区分にキャピラリー１４を挿入収納し、他方の区分で測定セル容器１２内の底部に比色計等で光を照射して被検液１６を測定するものである。
先に、特願２００２−２４１６５６号にて提案した技術では、被検液を移し替えたり零してしまうなどの不具合がない。しかるに、測定セル容器１２内を縦方向に２つの区分に分ける仕切板が必要であり、しかも測定セル容器１２の底部で２つの区分間で被検液の移動が自由な構造でなければならない。そこで、測定セル容器１２および仕切板を樹脂などで一体成形するには成形金型が極めて複雑で高価なものとなる。また、測定セル容器１２に仕切板を成形後に組み付ける構造では、組み付けの手間が必要となり、やはり製造コストが嵩むという不具合があった。 Previously, the technique proposed in Japanese Patent Application No. 2002-241656 has no inconvenience such as transfer or zeroing of the test solution. However, a partition plate that divides the inside of the measurement cell container 12 into two sections in the vertical direction is necessary, and the test liquid must be structured to be freely movable between the two sections at the bottom of the measurement cell container 12. Therefore, in order to integrally form the measurement cell container 12 and the partition plate with a resin or the like, a molding die becomes extremely complicated and expensive. Further, in the structure in which the partition plate is assembled to the measurement cell container 12 after being molded, there is a problem that the labor for assembling is required and the manufacturing cost is increased.
本発明は、かかる従来技術の事情に鑑みてなされたもので、測定セル容器の成形が安価にでき、測定セルとして安価に量産が可能な比色計等の測定セルを提供することを目的とする。また、その使用方法を提供することを目的とする。 The present invention has been made in view of the circumstances of the prior art, and an object thereof is to provide a measurement cell such as a colorimeter that can be inexpensively molded as a measurement cell and can be mass-produced inexpensively. To do. Moreover, it aims at providing the usage method.
かかる目的を達成するために、本発明の比色計等の測定セルは、被検液を収容して、比色計等に装着されて光を照射される測定セルにおいて、測定セル容器の上部に開口部を設け、前記開口部よりキャピラリーを前記測定セル容器内に挿入収納できるようになし、また前記測定セル容器の深さを前記キャピラリーの長さよりも大とし、前記測定セル容器の前記キャピラリーの収納部分より上方位置に前記光が照射されるように構成されている。 In order to achieve such an object, a measurement cell such as a colorimeter of the present invention is a measurement cell that contains a test liquid and is mounted on the colorimeter or the like and irradiated with light. An opening is provided in the measurement cell container so that the capillary can be inserted and housed in the measurement cell container. The depth of the measurement cell container is larger than the length of the capillary, and the capillary of the measurement cell container is provided. The light is applied to a position above the storage portion.
そして、前記測定セル容器の上端の開口部に蓋体を水密構造に配設し、前記蓋体に前記キャピラリーを挿入できる孔を貫通穿設し、前記孔にゴム栓を圧入および抜き取り自在に構成しても良い。 A lid is provided in a watertight structure at the opening at the upper end of the measurement cell container, and a hole into which the capillary can be inserted is formed in the lid so that a rubber stopper can be press-fitted and removed. You may do it.
さらに、前記測定セル容器と前記蓋体が同じ樹脂で成形され、接着により前記測定セル容器に対して前記蓋体を水密構造に配設して構成することもできる。 Furthermore, the measurement cell container and the lid may be formed of the same resin, and the lid may be disposed in a watertight structure with respect to the measurement cell container by adhesion.
また、前記測定セル容器の前記キャピラリーが収納される収納部分を、前記光が照射される部分よりも、水平断面積が小さくなるように構成しても良い。 In addition, a storage portion in which the capillary of the measurement cell container is stored may be configured such that a horizontal cross-sectional area is smaller than a portion irradiated with the light.
そしてまた、前記測定セル容器の前記光が照射される部分の前記光の方向の寸法を、前記キャピラリーの収納部分で前記光の方向と平行な方向の寸法よりも、長くなるように構成しても良い。 Further, the dimension of the light direction of the portion irradiated with the light of the measurement cell container is configured to be longer than the dimension of the capillary storage portion in the direction parallel to the direction of the light. Also good.
そしてさらに、前記測定セル容器内に予め反応試薬を収容して前記蓋体およびゴム栓により前記測定セル容器を密閉して構成することもできる。 Further, the reaction cell can be stored in advance in the measurement cell container, and the measurement cell container can be sealed with the lid and the rubber stopper.
また、本発明の比色計等の測定セルの使用方法は、請求項１記載の比色計等の測定セルの前記測定セル容器内に反応試薬を収容し、検体を採取したキャピラリーを前記測定セル容器内に挿入収納し、前記測定セル容器内で前記反応試薬と前記検体を混合反応させて前記被検液とし、さらに前記比色計等で前記測定セル容器の前記キャピラリーの収納部分より上方位置に前記光を照射するようにして使用する。 The method of using a measurement cell such as a colorimeter according to the present invention comprises the step of measuring a capillary in which a reaction reagent is contained in the measurement cell container of the measurement cell such as a colorimeter and the sample is collected. Inserted and stored in a cell container, the reaction reagent and the sample are mixed and reacted in the measurement cell container to obtain the test solution, and further above the capillary storage part of the measurement cell container by the colorimeter or the like It is used by irradiating the position with the light.
そして、請求項２記載の比色計等の測定セルの前記測定セル容器内に予め反応試薬を収容し、検体を採取したキャピラリーを前記ゴム栓を抜き取って前記測定セル容器内に挿入収納し、前記ゴム栓を圧入して前記測定セル容器を振って前記反応試薬と前記検体を混合反応させて前記被検液とし、さらに前記比色計等で前記測定セル容器の前記キャピラリーの収納部分より上方位置に前記光を照射して使用しても良い。 The reaction reagent is previously stored in the measurement cell container of the measurement cell such as the colorimeter according to claim 2, the capillary from which the sample is collected is removed from the rubber stopper, and inserted and stored in the measurement cell container. The rubber plug is press-fitted and the measurement cell container is shaken to mix and react the reaction reagent and the sample to obtain the test solution. Further, the colorimeter or the like above the capillary storage part of the measurement cell container. The position may be used by irradiating the light.
請求項１記載の比色計等の測定セルおよび請求項７記載の使用方法は、検体と反応試薬を測定セル容器内で混合することができ、従来の試験管などで混合して生成した被検液を移し替える手間が省かれ、それだけ測定作業が容易であるとともに、移し替える際に被検液を零す虞がない。そして、測定セル容器内に収容されたキャピラリーより上部の被検液に光を照射して測定するので、キャピラリーが測定の邪魔となることがない。さらに、測定セル容器は、収容されたキャピラリーを所定位置とする構造を必要とせず、簡単な金型で樹脂成形が可能である。 A measuring cell such as a colorimeter according to claim 1 and a method of using according to claim 7 can mix a sample and a reaction reagent in a measuring cell container, and can be produced by mixing in a conventional test tube or the like. The trouble of transferring the test solution is saved, and the measurement work is facilitated as much, and there is no possibility of spilling the test solution when transferring the test solution. Since the measurement is performed by irradiating the test solution above the capillaries stored in the measurement cell container with light, the capillaries do not interfere with the measurement. Furthermore, the measurement cell container does not require a structure in which the accommodated capillary is in a predetermined position, and can be resin-molded with a simple mold.
請求項２記載の比色計等の測定セルは、測定セル容器の上端の開口部に蓋体を水密構造に配設し、蓋体に穿設した孔にゴム栓を圧入するので、ゴム栓の圧入により確実に密閉構造にでき、しかもゴム栓を抜き取ることで、容易にキャピラリーを挿入することができる。 A measuring cell such as a colorimeter according to claim 2 is provided with a lid in a watertight structure at an opening at the upper end of the measuring cell container, and a rubber plug is press-fitted into a hole formed in the lid. By press-fitting, the sealing structure can be surely formed, and the capillary can be easily inserted by removing the rubber stopper.
請求項３記載の比色計等の測定セルは、測定セル容器と蓋体が同じ樹脂で成形されるので、接着による水密構造は、温度変化等に対する耐久性が高い。 In the measurement cell such as a colorimeter according to claim 3, since the measurement cell container and the lid are formed of the same resin, the watertight structure by adhesion has high durability against temperature change and the like.
請求項４記載の比色計等の測定セルは、測定セル容器のキャピラリーの収納部分の水平断面積が小さくなるようにしたので、被検液が少量でも、液面位置が高くなり、キャピラリーの上部にある被検液に光を照射して測定することが可能となる。 In the measurement cell such as a colorimeter according to claim 4, since the horizontal cross-sectional area of the capillary storage portion of the measurement cell container is reduced, the liquid surface position is increased even if the amount of the test liquid is small, and the capillary It becomes possible to measure by irradiating the test liquid in the upper part with light.
請求項５記載の比色計等の測定セルは、測定セル容器の光が照射される部分を、照射する光の方向の寸法を長くしたので、被検液を光が通過する距離が長くなり、それだけ測定精度の向上が図れる。 In the measurement cell such as the colorimeter according to claim 5, the dimension of the direction of the light to be irradiated is increased in the portion of the measurement cell container irradiated with the light, so that the distance through which the light passes through the test solution is increased. Therefore, the measurement accuracy can be improved.
請求項６記載の比色計等の測定セルおよび請求項８記載の使用方法は、測定セル容器がゴム栓の圧入および抜き取りにより、密閉およびキャピラリーの挿入自在となるので、反応試薬を予め収容しておくことができる。そこで、予め反応試薬を収容した測定セル容器内に、ゴム栓を抜き取って検体を採取したキャピラリーを挿入収容させ、再びゴム栓を圧入して密閉し、容易かつ確実に検体と反応試薬を混合して被検液とすることができる。そして、この混合の際に、反応試薬または被検液を零す虞がない。 A measuring cell such as a colorimeter according to claim 6 and a method of using according to claim 8 are characterized in that the measuring cell container can be hermetically sealed and the capillary can be inserted by press-fitting and removing a rubber stopper. I can keep it. Therefore, the capillary in which the sample was collected by removing the rubber stopper was inserted and housed in the measurement cell container in which the reaction reagent had been stored in advance, and then the rubber stopper was pressed in and sealed again. Can be used as a test solution. In this mixing, there is no possibility of spilling the reaction reagent or the test solution.
以下、本発明の比色計等の測定セルの第１実施例につき、図１ないし図５を参照して説明する。図１は、本発明の比色計等の測定セルの外観斜視図である。図２は、図１に示す測定セルの縦断面図である。図３は、キャピラリーに検体を採取する手順を示す図である。図４は、検体を採取したキャピラリーを測定セル内に挿入収納する手順を示す図である。図５は、測定セル内で検体と反応試薬を混合反応させるために、測定セルを良く振る手順を示す図である。図１ないし図５において、図９と同じまたは均等な部材には同じ符号を付けて重複する説明を省略する。 Hereinafter, a first embodiment of a measuring cell such as a colorimeter of the present invention will be described with reference to FIGS. FIG. 1 is an external perspective view of a measuring cell such as a colorimeter of the present invention. FIG. 2 is a longitudinal sectional view of the measurement cell shown in FIG. FIG. 3 is a diagram showing a procedure for collecting a sample in a capillary. FIG. 4 is a diagram showing a procedure for inserting and storing a capillary from which a sample is collected in a measurement cell. FIG. 5 is a diagram showing a procedure for shaking the measurement cell well in order to mix and react the specimen and the reaction reagent in the measurement cell. 1 to 5, the same or equivalent members as in FIG.
図１および図２に示すごとく、本発明の比色計等の測定セル３０は、測定セル容器３２が透明な例えばポリカーボネートなどにより樹脂成形され、上端部が開口された縦長で水平断面が略長方形となる角形の容器状であり、上端の開口部に同じ樹脂成形された蓋体３４が適宜に嵌合されるとともに、接着剤により固着される。また、蓋体３４には、キャピラリー１４が挿入できる孔３６が貫通穿設され、この孔３６にゴム栓３８が圧入される。なお、測定セル容器３２の内側の深さは、キャピラリー１４の長さよりも大きく設定される。また、測定セル容器３２と蓋体３４は、接着部が水密構造とされ、ゴム栓３８を圧入すると、測定セル容器３２内は密閉状態となる。 As shown in FIG. 1 and FIG. 2, the measuring cell 30 such as a colorimeter of the present invention has a measuring cell container 32 formed of a transparent resin, such as polycarbonate, and is vertically long with an upper end opened and has a substantially rectangular horizontal cross section. A lid 34 made of the same resin is appropriately fitted into the opening at the upper end and is fixed by an adhesive. In addition, a hole 36 through which the capillary 14 can be inserted is formed in the lid 34, and a rubber plug 38 is press-fitted into the hole 36. The depth inside the measurement cell container 32 is set larger than the length of the capillary 14. In addition, the measurement cell container 32 and the lid 34 have a watertight structure at the bonding portion, and when the rubber plug 38 is press-fitted, the measurement cell container 32 is hermetically sealed.
かかる構成からなる比色計等の測定セル３０の測定セル容器３２内に、反応試薬が予め収容され、ゴム栓３８の圧入により密閉される。なお、収容される反応試薬の量は、キャピラリー１４の長さよりも高い液面位置となるように設定される。そして、図示しない比色計等に測定セル３０が装着されると、キャピラリー１４の収容部分よりも上部で、キャピラリー１４が収容された状態での反応試薬の液面位置以下に光が照射されるように構成される。 A reaction reagent is stored in advance in a measurement cell container 32 of a measurement cell 30 such as a colorimeter having such a configuration, and is sealed by press-fitting a rubber plug 38. The amount of the reaction reagent to be accommodated is set so that the liquid level is higher than the length of the capillary 14. When the measurement cell 30 is attached to a colorimeter or the like (not shown), light is irradiated below the liquid level position of the reaction reagent in the state where the capillary 14 is accommodated above the portion where the capillary 14 is accommodated. Configured as follows.
本発明の測定セル３０の使用方法は、まず図３に示すごとく、血液等の検体４０にキャピラリー１４の先端を当接させ、毛細管現象によりキャピラリー１４に検体４０を採取する。キャピラリー１４の寸法等により採取される検体４０の量は一定である。このようにして検体４０を採取したキャピラリー１４を、図４に示すごとく、ゴム栓３８を取り外して、孔３６から測定セル容器３２内に挿入収容させ、再びゴム栓３８を孔３６に圧入して測定セル容器３２内を密閉する。さらに、図５に示すごとく、手または適宜な機械などによって、キャピラリー１４が収容された測定セル３０を良く振り、検体を予め収容されている反応試薬と混合させて被検液とする。 In the method of using the measurement cell 30 of the present invention, first, as shown in FIG. 3, the tip of the capillary 14 is brought into contact with a specimen 40 such as blood, and the specimen 40 is collected in the capillary 14 by capillary action. The amount of the sample 40 collected according to the size of the capillary 14 is constant. As shown in FIG. 4, the capillary 14 from which the sample 40 has been collected in this manner is removed from the rubber plug 38 and inserted into the measurement cell container 32 through the hole 36, and the rubber plug 38 is press-fitted into the hole 36 again. The inside of the measurement cell container 32 is sealed. Further, as shown in FIG. 5, the measurement cell 30 in which the capillary 14 is accommodated is shaken well by hand or an appropriate machine, and the sample is mixed with the reaction reagent accommodated in advance to obtain a test solution.
そして、検体４０と反応試薬が良く混合されて被検液が生成された状態で、測定セル３０が比色計等の測定装置に装着されるが、図１および図２に示すごとく、測定セル容器３０内のキャピラリー１４の収容部分よりも上部の被検液に光が照射され、キャピラリー１４が測定の邪魔となることはない。しかも、被検液の移し替える手間が省かれるとともに、移し替える際に零す虞がない。そして、測定セル容器３２の光が照射される部分は、水平断面が長方形であり、測定セル容器３２に対して光を照射する位置がずれても、被検液内を光が通過する距離が大きく変化せず、大きな測定誤差を生ずることがない。 The measurement cell 30 is attached to a measuring device such as a colorimeter in a state in which the sample 40 and the reaction reagent are well mixed and a test liquid is generated. As shown in FIGS. Light is irradiated to the test solution above the accommodating portion of the capillary 14 in the container 30, and the capillary 14 does not interfere with the measurement. In addition, there is no need to transfer the test solution, and there is no risk of spilling when transferring. The portion of the measurement cell container 32 that is irradiated with light has a rectangular horizontal cross section, and even if the position where the light is irradiated to the measurement cell container 32 is shifted, the distance that the light passes through the test solution is long. It does not change greatly and does not cause a large measurement error.
次に、本発明の第２実施例を図６および図７を参照して説明する。図６は、本発明の比色計等の測定セルの第２実施例の外観斜視図である。図７は、図６に示す測定セルの縦断面図である。 Next, a second embodiment of the present invention will be described with reference to FIGS. FIG. 6 is an external perspective view of a second embodiment of a measuring cell such as a colorimeter of the present invention. FIG. 7 is a longitudinal sectional view of the measurement cell shown in FIG.
図６および図７に示す本発明の第２実施例で、図１および図２に示す本発明の第１実施例と相違するところは、測定セル５０の測定セル容器５２が上部の光が照射される水平断面が円形の太径部分５２ａと、これと連通してキャピラリー１４が収容される水平断面が円形の細径部分５２ｂとで形成されていることにある。キャピラリー１４が収容される部分を水平断面積が小さな円形の細径部分５２ｂとすることで、検体４０が少量であって、被検液が少量の場合でも被検液の液面位置を高くすることができる。そして、光が照射される部分を水平断面の大きな太径部分５２ａとすることで、光が被検液中を長い距離通過でき、被検液により透過・吸収・錯乱されるなどの性質により大きく影響を受けた光が受光部２４で受光でき、それだけ測定精度が良い。 The second embodiment of the present invention shown in FIGS. 6 and 7 is different from the first embodiment of the present invention shown in FIGS. 1 and 2 in that the measurement cell container 52 of the measurement cell 50 is irradiated with the upper light. The horizontal cross section is formed by a circular large-diameter portion 52a, and the horizontal cross-section in which the capillary 14 is accommodated in communication therewith is formed by a circular small-diameter portion 52b. By making the portion in which the capillary 14 is accommodated into a circular small-diameter portion 52b having a small horizontal cross-sectional area, the liquid level position of the test liquid is increased even when the amount of the specimen 40 is small and the test liquid is small. be able to. Then, by making the portion irradiated with light a large-diameter portion 52a having a large horizontal section, the light can pass through the test solution for a long distance, and is greatly increased due to properties such as transmission, absorption, and confusion by the test solution. The affected light can be received by the light receiving unit 24, and the measurement accuracy is improved accordingly.
なお、第２実施例のごとく、光を照射する部分の水平断面形状が円形であると、光の照射する位置が横方向にずれると、光が被検液を通過する距離が変化し、測定誤差を生ずることになる場合もある。そこで、光の照射位置がずれないことが望ましい。 Note that, as in the second embodiment, if the horizontal cross-sectional shape of the portion that irradiates light is circular, the distance that the light passes through the test solution changes when the position where the light irradiates shifts in the lateral direction, and measurement An error may occur. Therefore, it is desirable that the light irradiation position does not shift.
さらに、本発明の第３実施例を図８を参照して説明する。図８は、本発明の比色計等の測定セルの第３実施例の外観斜視図である。 Furthermore, a third embodiment of the present invention will be described with reference to FIG. FIG. 8 is an external perspective view of a third embodiment of a measuring cell such as a colorimeter of the present invention.
図８に示す本発明の第３実施例で、第２実施例と相違するところは、測定セル６０の測定セル容器６２が、上部の光が照射される部分が光りの照射方向に長く水平断面形状が長方形の長方形部分６２ａと、これに連通してキャピラリー１４が収容される水平断面が円形の細径部分６２ｂとで形成されていることにある。キャピラリー１４が収容される部分を水平断面形状が小さな円形の細径部分６２ｂとすることで、少量の被検液であっても液面位置を高くできることは、第２実施例と同様である。そして、光が照射される部分を光の照射方向に水平断面が長い長方形の長方形部分６２ａとすることで、少ない被検液であっても光の通過距離を第２実施例よりも長くでき、より測定精度の向上が図られる。 The third embodiment of the present invention shown in FIG. 8 is different from the second embodiment in that the measurement cell container 62 of the measurement cell 60 has a horizontal section in which the portion irradiated with the light is long in the light irradiation direction. The shape is that the rectangular portion 62a having a rectangular shape and the horizontal cross section in which the capillary 14 is accommodated in communication with the rectangular portion 62a are formed with a circular small diameter portion 62b. As in the second embodiment, the liquid level position can be increased even with a small amount of the test liquid by making the portion in which the capillary 14 is accommodated into a circular thin diameter portion 62b having a small horizontal cross-sectional shape. And by making the portion irradiated with light a rectangular portion 62a having a long horizontal cross section in the light irradiation direction, the light passing distance can be made longer than in the second embodiment even with a small amount of test liquid, The measurement accuracy can be further improved.
なお、上記実施例において、測定セル容器３２，５２，６２は成形の際に金型からの抜きを良くすべく、内側が僅かに上方で拡がるテーパ状に形成されても良い。また、測定セル３０，５０，６０は、収容されたキャピラリー１４より上方の光を照射する部分が透明であれば良く、他の部分が透明でなくても良い。さらに、上記実施例では、予め反応試薬を測定セル容器３２，５２，６２内に収容してゴム栓３８で密閉しているが、ゴム栓３８を取り外してキャピラリー１４を挿入するとともに反応試薬を測定セル容器３２，５２，６２内に収容させるようにしても良い。そしてまた、検体は血液に限られず、比色計等による測定が有益であれば、いかなるものであっても良い。ここで、検体に応じて反応試薬が適宜に選定されることは勿論である。さらにまた、上記実施例では測定セル容器３２，５２，６２の上端の開口部が蓋体３４とゴム栓３８によって閉塞されているが、蓋体３４自体をゴム等の弾性材で形成して、蓋体３４自体の圧入により測定セル容器３２，５２，６２の上端の開口部を閉塞および開成自在としても良い。そしてさらに、測定セル容器３２，５２，６２の上端の開口部にシール等を貼着して閉塞し、シール等を剥がして開成するようにしても良い。ここで、シール等は貼着と剥離とが数回可能なものを用いれば良い。 In the above-described embodiment, the measurement cell containers 32, 52, and 62 may be formed in a tapered shape in which the inner side slightly expands upward in order to improve the removal from the mold during molding. Moreover, the measurement cells 30, 50, and 60 may be transparent so long as the portion irradiating light above the accommodated capillary 14 is transparent, and other portions may not be transparent. Further, in the above embodiment, the reaction reagent is previously stored in the measurement cell containers 32, 52, and 62 and sealed with the rubber plug 38, but the rubber plug 38 is removed and the capillary 14 is inserted and the reaction reagent is measured. You may make it accommodate in cell container 32,52,62. The sample is not limited to blood, and any sample may be used as long as measurement by a colorimeter or the like is useful. Here, as a matter of course, the reaction reagent is appropriately selected according to the specimen. Furthermore, in the above embodiment, the opening at the upper end of the measurement cell containers 32, 52, 62 is closed by the lid 34 and the rubber plug 38, but the lid 34 itself is formed of an elastic material such as rubber, The opening at the upper end of the measurement cell containers 32, 52, 62 may be closed and opened by press-fitting the lid 34 itself. Further, a seal or the like may be attached to the opening at the upper end of the measurement cell container 32, 52, or 62 to close it, and the seal or the like may be peeled off to be opened. Here, a seal or the like that can be stuck and peeled several times may be used.
１０，３０，５０，６０ 測定セル
１２，３２，５２，６２ 測定セル容器
１４ キャピラリー
１８、３４ 蓋体
３６ 孔
３８ ゴム栓
４０ 検体
５２ａ 太径部分
５２ｂ，６２ｂ 細径部分
６２ａ 長方形部分
10, 30, 50, 60 Measurement cell 12, 32, 52, 62 Measurement cell container 14 Capillary 18, 34 Lid 36 Hole 38 Rubber plug 40 Sample 52a Large diameter portion 52b, 62b Small diameter portion 62a Rectangular portion
Claims (8)
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| JP2003344474A JP4170189B2 (en) | 2003-10-02 | 2003-10-02 | Measuring cell such as a colorimeter and method of using the same |
| PCT/JP2004/014788 WO2005033677A1 (en) | 2003-10-02 | 2004-09-30 | Measurement cell for colorimeter and use method for the cell |
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| JP2003344474A JP4170189B2 (en) | 2003-10-02 | 2003-10-02 | Measuring cell such as a colorimeter and method of using the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2010038628A1 (en) * | 2008-09-30 | 2010-04-08 | Obata Toru | Gel particle generating instrument and gel particle measuring device using same |
| JP2011099795A (en) * | 2009-11-09 | 2011-05-19 | Shimadzu Corp | Liquid sample placement mechanism, apparatus and method for measuring refractive index using the same |
| EP1909094A4 (en) * | 2005-07-27 | 2014-12-17 | Sysmex Corp | CUVETTE |
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| CN100543453C (en) * | 2005-11-08 | 2009-09-23 | 白莉 | Thorium measuring solution and its colorimetric measuring tube |
| CN100510706C (en) * | 2005-11-08 | 2009-07-08 | 白莉 | Arsenic assaying liquid and colorimetric cylinder thereof |
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| DE2721942C3 (en) * | 1977-05-14 | 1980-02-07 | Bayer Ag, 5090 Leverkusen | Device for processing a measuring liquid for use in analytical optical devices |
| JPS57182147A (en) * | 1981-05-01 | 1982-11-09 | Agency Of Ind Science & Technol | Analysis of olefin and its device |
| JPS58191956A (en) * | 1982-05-06 | 1983-11-09 | Sunstar Giken Kk | Measuring apparatus of reaction state of test liquid |
| JP4446592B2 (en) * | 2000-12-25 | 2010-04-07 | シスメックス株式会社 | Liquid sample measurement unit and automatic liquid sample analyzer equipped with the same |
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| EP1909094A4 (en) * | 2005-07-27 | 2014-12-17 | Sysmex Corp | CUVETTE |
| EP3206011A1 (en) * | 2005-07-27 | 2017-08-16 | Sysmex Corporation | Cuvette |
| EP3421969A1 (en) * | 2005-07-27 | 2019-01-02 | Sysmex Corporation | Cuvette |
| WO2010038628A1 (en) * | 2008-09-30 | 2010-04-08 | Obata Toru | Gel particle generating instrument and gel particle measuring device using same |
| JP2011099795A (en) * | 2009-11-09 | 2011-05-19 | Shimadzu Corp | Liquid sample placement mechanism, apparatus and method for measuring refractive index using the same |
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