JP2004093548A - Disc-like optical recording medium having microcapillary and sample analyzing apparatus using the disc-like optical recording medium - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a disc-like optical recording medium satisfying two requirements i.e. detection of an enormous quantity of DNA information and rapid, correct record analysis which are indispensable conditions for establishing bioinformatics, and sample analyzing apparatus using the disc-like optical recording medium. <P>SOLUTION: An optical recording area 15 and an analyzing area 14 are formed on the surface of a disc-like substrate 11. A microcapillary 23, which has a sample inlet at least at one end thereof, is formed on the analyzing area 14. By using the microcapillary 23, isolation and detection of sample components can be conducted, and the analyzed information can be stored on the optical recording area 15. <P>COPYRIGHT: (C)2004,JPO

Description

【図面の簡単な説明】
【図１】本発明の一実施形態であるマイクロキャピラリーを有するディスク状光記録媒体の平面図である。
【図２】上記ディスク状光記録媒体の表面に形成されたマイクロキャピラリーの拡大模式図である。
【図３】上記ディスク状光記録媒体の模式断面図である。
【図４】図３における光記録領域と分析領域の境界部分の拡大図である。
【図５】キャピラリーカバーの代替として、張り合わせ構造の記録層を持たない上面片側ディスクを用いた例を示す図である。
【図６】本発明の別の実施形態であるマイクロキャピラリーを有するディスク状光記録媒体の平面図である。
【図７】上記ディスク状光記録媒体の表面に形成されたマイクロキャピラリーの拡大模式図である。
【図８】本発明の更に別の実施形態であるマイクロキャピラリーを有するディスク状光記録媒体の平面図である。
【図９】上記ディスク状光記録媒体に形成された分析領域を拡大して示す部分平面図である。
【図１０】本発明の試料分析装置の一実施形態を示す概略構成図である。
【図１１】実施例１において、本発明のディスク状光記録媒体及び試料分析装置を用いてＤＮＡ標準分子量サンプルを電気泳動して、検出した結果を示す図である。
【図１２】実施例２において、本発明のディスク状光記録媒体及び試料分析装置を用いてＤＮＡ標準分子量サンプルを電気泳動して、検出した結果を示す図である。
【図１３】実施例３において、本発明のディスク状光記録媒体及び試料分析装置を用いてＰＣＲによる微生物遺伝子増幅産物を分離、検出した結果を示す図である。
【図１４】実施例４において、本発明のディスク状光記録媒体及び試料分析装置を用いてＤＮＡ標準分子量サンプルを分離して、検出した結果を示す図である。
【符号の説明】
１、２、３．マイクロキャピラリーを有するディスク状光記録媒体
１１．基板
１２．中心穴
１３．ディスク保持部
１４．分析領域
１５．光記録領域
２１、２４．試料移送用キャピラリー
２１ａ、２１ｂ、２４ａ．試料注入口
２２、２５．試料分離用キャピラリー
２３、２６．マイクロキャピラリー
２７、２８．電極
３１．凹部
３２．溝
３３．キャピラリーカバー
３４．上面片側ディスク
４１．案内溝
４２．記録層
４３．反射層
４４．保護膜
５１．レーザ光源
５２．マルチレンズ
５３．ビームスプリッタ
５４．コリメータ
５５．対物レンズ
５６．集光レンズ
５７．光検出素子
５８．増幅回路
５９．サーボ・信号処理ＬＳＩ
６０．レーザ駆動回路
６１．ＣＣＤカメラ[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention, on the surface of a disk-shaped substrate, sample components, for example, biochemistry, microcapillary used for separation and detection of bio-related high-molecular substances and drugs related to medical and pharmacy, and to record the detection information The present invention relates to a disk-shaped optical recording medium having an optical recording area, and a sample analyzer using the disk-shaped optical recording medium.
[0002]
[Prior art]
In the field of life science, the genomes (all gene information) of various organisms including humans are being decoded at a rapid pace, and the results of the decoding are expected to improve new animal and plant varieties.
[0003]
Also, among single species, it is known that the nucleotide sequence of the genome is slightly different depending on individuals reflecting the diversity (such diversity is referred to as genomic polymorphism or simply polymorphism). For example, in humans, the probability that the nucleotide sequence differs between individuals is about 0.1%. This means that a polymorphism is found at one place for every 1000 bases, and appears at about 3 million bases in the entire human genome (about 3 billion bases).
[0004]
Therefore, by analyzing the genetic information of individual humans, the most appropriate medical treatments and medications based on their own genetic information, which go beyond medical techniques that have never been available for symptomatic treatment, are called tailor-made medical treatments. It is hoped that it will be possible to realize extremely effective disease prevention, medication and treatment while eliminating waste of medication and treatment. For example, a single nucleotide polymorphism (SNP: Single Nucleotide Polymorphism) that differs by only one nucleotide may affect the expression level of a gene or cause an amino acid mutation. Analyzes are expected to find treatments and drugs that are suitable for individuals, and are expected to lead to the establishment of tailor-made medicine.
[0005]
On the other hand, the history of technological development of human genome sequencing is also the history of increasing the absolute amount of base decoding. That is, despite the fact that only 100 bases or less could be decoded annually in the world in the 70's, the Sanger method, the capillary array electrophoresis method, etc. were developed in only 30 years, and the giga year (G = 10 ⁹ ) Sequencing of bases became possible. In particular, capillary electrophoresis is an analysis method that can perform DNA analysis at high speed, with high sensitivity, and with high accuracy, and has the advantages of simple measurement, simultaneous analysis of multiple samples, and easy automation. .
[0006]
[Problems to be solved by the invention]
However, in order to realize customized medicine from genomic drug discovery in the future, tera (T = 10 ¹² ) Base, peta (T = 10 ^Fifteen ) Base, let alone exa (E = 10), which is global human DNA information ¹⁸ ) Is said to be required, and the advanced use of information science and technology is required for recording and analyzing such enormous amounts of base information. This is the reason why the necessity of bioinformatics (bioinformatics) has been promoted in recent years. An information processing system that accurately records, transmits, and analyzes the enormous amount of information obtained by a DNA sequencer has been developed. is needed.
[0007]
Accordingly, an object of the present invention is to detect a huge amount of DNA information, which is an essential condition for the establishment of bioinformatics, and a disc-shaped optical recording medium capable of satisfying two requirements of quick and accurate recording analysis, And a sample analyzer using the disk-shaped optical recording medium.
[0008]
[Means for Solving the Problems]
In order to achieve the above object, one aspect of the present invention has a disk-shaped substrate, an optical recording area formed on the surface of the substrate, and an analysis area. An object of the present invention is to provide a disk-shaped optical recording medium having a microcapillary, wherein a microcapillary having an entrance is formed.
[0009]
In the disc-shaped optical recording medium, the microcapillary is formed radially with respect to the center of the substrate, the sample inlet is formed at an end of the substrate on the center side, and the substrate is It is preferable that the sample component is separated by centrifugal force when rotated.
[0010]
Further, the microcapillary has electrodes formed at at least two places in its path, and separates the sample components by applying a voltage between the electrodes and electrophoresing the sample components. Is preferred.
[0011]
Further, the microcapillary includes a sample transfer capillary and a sample separation capillary, and the sample transfer capillary has a sample inlet at at least one end thereof, and intersects with the sample separation capillary in the middle of a path. It is preferable that the other end is connected to the sample separation capillary at the other end.
[0012]
Furthermore, it is preferable that holes for buffer solution injection or storage are formed at both ends of the sample separation capillary.
[0013]
Furthermore, it is preferable that the microcapillary is constituted by a groove formed on the surface of the substrate, and a cover closing an opening of the groove.
[0014]
Furthermore, it is preferable that the optical recording area and the analysis area are formed on the same surface of the disk-shaped substrate.
[0015]
Furthermore, it is preferable that the optical recording layer formed in the optical recording area is a write-once recording layer.
[0016]
According to the invention, the sample is separated and detected by the microcapillary formed in the analysis area on the disk-shaped substrate surface, and the analysis information is recorded and stored in the optical recording area formed on the disk-shaped substrate surface. Therefore, the process from separation and detection of the sample to recording of the analysis information can be performed on one disk.
[0017]
Another aspect of the present invention is a disc-shaped optical recording medium having the microcapillary, a turntable for supporting and rotating the disc-shaped optical recording medium, and irradiating the disc-shaped optical recording medium with laser light. A laser beam irradiating unit, a laser beam receiving unit for receiving a reflected beam of the laser beam applied to the optical recording area of the optical recording medium, and a laser beam applied to the analysis area of the optical recording medium. And an image detecting means for detecting an image.
[0018]
According to the invention, it is possible to easily detect the sample components separated by the microcapillary on the disk-shaped optical recording medium and record the analysis information thereof.
[0019]
BEST MODE FOR CARRYING OUT THE INVENTION
Hereinafter, the present invention will be described in detail with reference to the drawings.
[0020]
1 to 4 show an embodiment of a disc-shaped optical recording medium having a microcapillary of the present invention. FIG. 1 is a plan view of a disk-shaped optical recording medium having a microcapillary, FIG. 2 is an enlarged schematic view of a microcapillary formed on the surface of the disk-shaped optical recording medium, and FIG. 3 is a schematic diagram of the disk-shaped optical recording medium. FIG. 4 is an enlarged view of a boundary portion between the optical recording area and the analysis area.
[0021]
As shown in FIG. 1, the disc-shaped optical recording medium 1 has an optical recording area 15 in an outer peripheral area on one surface of a disc-shaped substrate 11 and a plurality of microcapillaries 23 in an inner peripheral area in contact with the optical recording area. A plurality of analysis regions 14 are formed, and a center hole 12 for holding a disk and a disk holding portion 13 are formed in a further inner peripheral portion of the analysis region.
[0022]
The microcapillary 23 formed in the analysis region 14 is described in a literature or the like (Chao-Xuan Zhang and Andreas Manz, “Narrow Sample Channel Injectors for Capillary Electrolysis on Microchips. Like a cross-shaped electrophoresis capillary, the capillary comprises a short sample transfer capillary 21 and a long sample separation capillary 22, and as shown in FIG. 2, the sample transfer capillary 21 and the sample separation capillary 22 are used. Are formed so as to intersect and connect at a predetermined angle in the middle of the route. In the present invention, the sample transfer capillary 21 and / or the sample separation capillary 22 are provided on the substrate 11 so that the sample can be transferred and / or separated by centrifugal force when the substrate 11 is rotated. It is preferable to be formed radially with respect to the center. Here, the term “radial” includes a shape that goes outward at an angle inclined with respect to the radial direction, and a shape that goes outward in a spiral shape.
[0023]
In this embodiment, the sample separation capillary 22 is formed in a shape going outward from the center of the substrate 11 at a slightly inclined angle with respect to the radial direction, and the sample component is centrifugally generated by the rotation of the disk. It can be separated.
[0024]
Further, sample injection ports 21a and 21b are formed at both ends of the sample transfer capillary 21, so that a sample can be injected into the microcapillary from the sample injection ports. On the other hand, a buffer solution injection hole 22a is formed at one end of the sample separation capillary 22, and a buffer solution storage hole 22b is formed at the other end, so that the buffer solution can be injected from the buffer solution injection hole. It has become.
[0025]
As shown in FIG. 3, the microcapillary 23 is formed by covering a concave portion 31 formed in a predetermined shape with a capillary cover 33 on one surface of the substrate 11 (the side opposite to the laser beam incident surface). That is, as shown in FIG. 4, a fine groove 32 of a predetermined length having a sample injection port and a hole for buffer solution injection or storage at both ends is formed in the capillary forming portion on the bottom surface of the concave portion 31. By covering the opening of the groove 32 with a capillary cover 33, a microcapillary is formed. In the capillary cover 33, through holes of a predetermined size are formed at respective positions corresponding to the sample injection port and the hole for buffer solution injection or storage, and sample injection is performed through the through hole. The inlet and the hole for buffer solution injection or storage are open when viewed from above, but the capillary forming portion is covered with a capillary cover and is sealed.
[0026]
As shown in FIG. 5, as an alternative to the capillary cover 33, an upper surface single-sided disk 34 (dummy layer) having no laminated recording layer may be used.
[0027]
The width and depth of the groove 32 may be a width and a depth that exhibit a capillary phenomenon so that a sample or a buffer solution can be sufficiently sucked and filled in the entire capillary, and a uniform width over the entire area of the capillary. And a depth. Specifically, the width is preferably 20 to 300 μm and the depth is 10 to 200 μm, and the width is preferably 50 to 150 μm and the depth is more preferably 20 to 40 μm.
[0028]
The length of the groove 32 should be such that an appropriate amount of sample can be easily transferred in the case of forming a sample transfer capillary, and is usually preferably 2 to 30 mm, more preferably 3 to 5 mm. In addition, a capillary for forming a sample separation capillary may have a length sufficient for sufficient separation by centrifugal force or electrophoresis, and is usually preferably 10 to 100 mm, more preferably 15 to 50 mm.
[0029]
The size (diameter) of the sample injection port and the buffer solution injection or storage hole formed at both ends of the groove 32 is such that the sample or buffer solution can be injected by an injection instrument such as a micropipette, and Any size may be used as long as it has a function of a liquid reservoir, and specifically, a size having a diameter of 1 to 5 mm. Further, it is preferable that the depth is equal to the depth of the groove 32.
[0030]
In the present invention, electrodes may be formed at both ends of the sample transfer capillary 21 and / or the sample separation capillary 22. The electrode is drawn out to the upper surface of the capillary cover through a sample injection port provided in the capillary cover (or a disk on one side of the upper surface) and a through hole having the same diameter as the hole for buffer solution injection or storage.
[0031]
For example, when electrodes are formed at both ends of the sample transfer capillary 21, by applying a voltage between the electrodes, the sample injected into the sample injection port 21 a (or 21 b) is connected to the sample transfer capillary 21. It can be transported to the intersection with the capillary 22 for sample separation. When electrodes are formed at both ends of the sample separation capillary 22, separation of sample components by electrophoresis can be performed by applying a voltage between the electrodes.
[0032]
On the other hand, in the optical recording area 15, an information recording section similar to a normal CD-R or DVD-R is formed. That is, as shown in FIGS. 4 and 5, the information recording section 45 includes a guide groove 41 formed at a predetermined pitch on the surface of the substrate 11 opposite to the laser light incident surface, and an organic dye laminated on the surface. And a reflective layer 43, the surface of which is covered with a protective film 44.
[0033]
As a material of the substrate 11 constituting the disk-shaped optical recording medium of the present invention, a polycarbonate resin used for an ordinary optical recording medium is preferably used. In addition, the capillary cover 33 and the upper surface one-sided disk 34 and the like may be made of polycarbonate or a material (for example, acrylic resin or the like) sufficiently transmitting a laser beam corresponding thereto.
[0034]
The shape of the substrate (disk) is not limited to a circle but may be a square or the like, but the final outer shape or the like is basically based on Japanese Industrial Standards (JIS), a compact disk digital audio system (S8605-1993 (IEC908)). : 1987)), and preferably conforms to the standard of DVD FLLC (Format / Logo Licensing Corporation).
[0035]
6 and 7 show another embodiment of the disc-shaped optical recording medium having the microcapillary of the present invention. FIG. 6 is a plan view of a disk-shaped optical recording medium having a microcapillary, and FIG. 7 is an enlarged schematic view of a microcapillary formed on the surface of the disk-shaped optical recording medium. In the following description of the embodiment, the same reference numerals are given to substantially the same portions as those of the above embodiment, and the description thereof will be simplified or omitted.
[0036]
As shown in FIG. 6, the disc-shaped optical recording medium 2 is such that the positions of an optical recording area 15 and an analysis area 14 formed on one surface of a substrate 11 are opposite to those of the disc-shaped optical recording medium 1. Are different. That is, in the disc-shaped optical recording medium 2, the analysis area 14 is formed in the outer peripheral area of the substrate 11, and the optical recording area 15 is formed in the inner peripheral area in contact with the analysis area 14.
[0037]
The microcapillary 23 'formed in the analysis area 14 is composed of a shorter sample transfer capillary 21' and a longer sample separation capillary 22 'as described above. However, the sample separation capillary 22 ′ is formed in an arc shape concentric with the outer periphery at a portion slightly inside the outer periphery of the substrate 11, and the sample transfer capillary 21 ′ is formed as the sample separation capillary 22 ′. , Obliquely intersecting with each other, one end thereof is located near the center of the substrate 11 and the other end is located near the outer periphery. As a result, the sample transfer capillary 21 'can transfer the sample to the sample separation capillary 22' by the centrifugal force accompanying the rotation of the disk. In addition, electrodes (not shown) are formed at both ends of the sample separation capillary 22 'so that sample components can be separated by electrophoresis.
[0038]
8 and 9 show still another embodiment of the disc-shaped optical recording medium having the microcapillary of the present invention. FIG. 8 is a plan view of a disc-shaped optical recording medium having a microcapillary, and FIG. 9 is an enlarged view of an analysis area formed on the disc-shaped optical recording medium.
[0039]
As shown in FIG. 8, the disc-shaped optical recording medium 3 has an optical recording area 15 in an outer peripheral area on one surface of a disc-shaped substrate 11 and a plurality of microcapillaries 26 in an inner peripheral area in contact with the optical recording area. And the formed analysis area 14. In this embodiment, an identification barcode 16 is formed on the disk holding unit 13.
[0040]
As shown in FIG. 9, the microcapillary 26 formed in the analysis area 14 includes a short sample transfer capillary 24 radially and spirally extending from the center side of the substrate 11 toward the outer diameter direction, and a sample transfer capillary 24. The capillary 24 includes a longer sample separation capillary 25 that extends radially outward from the end of the capillary 24 continuously from the end in the outer diameter direction.
[0041]
Then, an inner end of the sample transfer capillary 24, an end of a portion where the sample transfer capillary 24 and the sample separation capillary 25 are connected, and an outer end of the sample separation capillary 25. A hole for injecting and storing a sample and / or a buffer solution is formed therein. Incidentally, a sample injection port 24a is formed at the end on the inner diameter side of the sample transfer capillary 24.
[0042]
Electrodes 27 and 28 are formed at the end of the portion where the sample transfer capillary 24 and the sample separation capillary 25 are connected, and at the outer diameter end of the sample separation capillary 25, By applying a voltage between the electrodes 27 and 28, sample components can be separated by electrophoresis.
[0043]
Therefore, according to the disk-shaped optical recording medium 3 having the microcapillary, the sample injected into the sample injection port 24a is moved along the sample transfer microcapillary 24 by the centrifugal force generated by the rotation of the disk-shaped optical recording medium 3. The sample moves in the outer diameter direction and reaches the inner end of the sample separation capillary 25.
[0044]
Then, by applying a voltage between the electrodes 27 and 28, the sample components can be moved by electrophoresis in the sample separation capillary 25 to separate the sample components. In addition, by performing the above-mentioned electrophoresis while rotating the disc-shaped optical recording medium 3, the centrifugal force generated by the rotation of the disc-shaped optical recording medium 3 and the electrophoresis are used together to separate the sample components. You can also.
[0045]
Then, by using a sample analyzer or the like described later, the sample components separated in the sample separation capillary 25 can be detected by an optical means such as a laser, and information on separation / detection is sequentially obtained. Alternatively, they can be collectively recorded in the optical recording area of the disc-shaped optical recording medium.
[0046]
Accordingly, since a single disk-shaped optical recording medium can perform from separation and detection of sample components to storage of the record of the analysis information and the like, data management and the like are very easy.
[0047]
In the present invention, the recording layer formed in the optical recording area is preferably a write-once recording layer. That is, when the recording layer is a storage mode using an organic dye similar to a CD-R or DVD-R, information once written on the recording layer cannot be rewritten, thereby preventing data falsification and the like. be able to.
[0048]
The optical recording area contains information on the reagent (component, expiration date, intended use, etc.) filled in the sample separation capillary, information on the sample to be analyzed (component, concentration, manufacturer or preparation conditions), and experimental conditions. (Sample usage, disk rotation speed, voltage, temperature, detection wavelength, etc.) and a program for executing the program may be recorded in advance. As a result, it is possible to accurately manage the experimental conditions and to prevent an operation error or the like.
[0049]
In the present invention, as shown in FIGS. 4 and 5, it is preferable that the optical recording area and the analysis area are formed on the same surface of the disk-shaped substrate. This makes it possible to share the light source for sample component detection and the light source for recording / reading information in the optical recording area, and to detect the sample component and record information in the optical recording area with one light source. / Readout becomes possible.
[0050]
When sample components are separated using the disc-shaped optical recording medium of the present invention, for example, methyl cellulose (MC), hydroxyethyl cellulose (HEC), hydroxypropyl cellulose (HPC), hydroxypropyl methyl cellulose It is used after filling a buffer solution containing a cellulose derivative such as (HPMC), a polymer of polyethylene glycol, polyethylene oxide, polyacrylamide, agarose, an acrylamide derivative, or the like. The buffer solution can be filled by capillary action by dropping an appropriate amount of buffer solution into a sample injection port formed at the end of the capillary or a hole for buffer solution injection or storage.
[0051]
The sample component is not particularly limited, but high molecular substances related to biochemistry such as drug molecules, amino acids, peptides, DNA, oligonucleotides, and relatively large proteins can be preferably exemplified.
[0052]
The sample component is labeled with a fluorescent dye having an absorption band around the laser wavelength as necessary so that it can be detected at 780 nm, which is the laser wavelength of CD-R, or 630 nm, which is the laser wavelength of DVD-R. You. The fluorescent dye may be appropriately selected depending on the type of the sample component and the laser wavelength used for detection. For example, when detection is performed at 780 nm, which is the CD-R laser wavelength, the fluorescent dye has an absorption band at 750 to 810 nm. It is preferable to use a fluorescent dye, and in the case of performing detection at 630 nm, which is the DVD-R laser wavelength, it is preferable to use a fluorescent dye having an absorption band at 600 to 660 nm. The labeling of the sample component with a fluorescent dye may be performed according to a known method. Therefore, a sample component may be labeled in advance with a fluorescent dye and then subjected to analysis, and the sample component may be added to a buffer containing a polymer or the like filled in the capillary by selecting an appropriate fluorescent dye. Thus, labeling can be performed simultaneously with separation of sample components.
[0053]
For example, when DNA or oligonucleotide is used as a sample component, TOTO-3, IRDye41, IRDye800, or the like can be used as a fluorescent dye. Incidentally, TOTO-3 is a fluorescent dye having an absorption band near 630 nm, which is the laser wavelength of DVD-R, and is added to a buffer containing a polymer or the like to be filled in the capillary, so that it can be separated at the same time as separation. Labeling (staining) of DNA and oligonucleotides can be performed. Therefore, there is no need to separately label sample components, which is convenient.
[0054]
On the other hand, IRDye41 and IRDye800 are fluorescent dyes having an absorption band around 780 nm, which is the laser wavelength of CD-R.
[0055]
Next, the sample analyzer of the present invention will be described.
The sample analyzer of the present invention detects a sample component separated by using the above-mentioned disk-shaped optical recording medium, and records information on separation / detection, and supports and rotates the disk-shaped optical recording medium. A turntable, a laser light irradiating means for irradiating the disk-shaped optical recording medium with laser light, a laser light receiving means for receiving reflected light of the laser light applied to the optical recording area of the optical recording medium, and Image detecting means for detecting an image formed by the laser light applied to the analysis area of the optical recording medium.
[0056]
FIG. 10 is a schematic configuration diagram showing one embodiment of the sample analyzer of the present invention.
[0057]
In the figure, a disk-shaped optical recording medium 1 is supported by the turntable (not shown) and rotates. A photodetection recording means 50 is provided movably in the radial direction with respect to the disc-shaped optical recording medium 1.
[0058]
The light detection recording means 50 includes a laser light source 51 for emitting laser light by a laser driving circuit 60, a multi-lens 52 for converting the laser light into a parallel beam, and a beam for passing the laser emission light and extracting reflected light. A splitter 53, a collimator 54, an objective lens 55, a condenser lens 56 for condensing the reflected light split by the beam splitter 53, and a photodetector 57 for receiving the reflected light condensed by the condenser lens 56 And an amplifying circuit 58 for amplifying the optical signal detected by the photodetector 57, and a servo / signal processing LSI 59 for processing the optical signal input through the amplifying circuit. Further, a CCD (charge-coupled type) camera 61 oriented to image predetermined positions on the upper and lower surfaces of the disc-shaped optical recording medium 1 is provided.
[0059]
Therefore, the laser light emitted from the laser light source 51 is applied to a predetermined position of the disk-shaped optical recording medium 11 through the multi-lens 52, the beam splitter 53, the collimator 54, and the objective lens 55. This laser light is used, for example, as a light source for emitting the component separated by the sample separation capillary 25, and as a writing light source for recording analysis data in the optical recording area 15 of the disc-shaped optical recording medium 1. And is also used as a light source for reading information written in the optical recording area 15.
[0060]
When the laser light is used, for example, as a light source or the like for reading information written in the optical recording area 15, the laser light irradiated to the optical recording area 15 and reflected is extracted by the beam splitter 53, The light is applied to the light detection element 57 through the condenser lens 56. Then, the optical signal received by the light detecting element 57 is amplified by the amplifier circuit 58 and read by the servo / signal processing LSI 59. Further, the servo / signal processing LSI 59 also has a function of moving the laser light irradiation position from the laser light source 51 and controlling the laser light emission timing based on the read information.
[0061]
Therefore, according to this sample analyzer, after the sample component is separated by the sample separation capillary 22 of the disk-shaped optical recording medium 1, the sample separation capillary 22 is irradiated with laser light, and the pattern emitted by the laser light is emitted. Is captured by the CCD camera 61, and the light emission pattern is image-analyzed by a computer (not shown) to analyze the components.
[0062]
Further, by writing the analysis result thus obtained in the optical recording area 15 of the disk-shaped optical recording medium 1 with a laser beam, data can be stored on the same disk. Further, the written data is irradiated with a laser beam, the reflected light is extracted by a beam splitter 53, received by a photodetector 57 through a condenser lens 56, and the received optical signal is amplified by an amplifier circuit 58. -Data can also be read by reading with the signal processing LSI 59.
[0063]
【Example】
Hereinafter, the present invention will be described specifically with reference to examples. In the following example, the disc-shaped optical recording medium used was one in which a guide groove, a recording layer, a reflective layer, and the like were formed in a predetermined area of the substrate in a conventional manner, and a capillary was formed by machining. Were used.
[0064]
Example 1
DNA fragments were separated and detected using the disk-shaped optical recording medium having the structure shown in FIG.
[0065]
a) A concave portion 31 having a groove 32 for forming a capillary in an analysis region (a region where an optical recording layer is not formed) of a polycarbonate substrate 11 having an information recording portion similar to a CD-R formed in a predetermined region. Was formed. Specifically, as shown in FIG. 2, a sample transfer capillary 21 (3.5 mm in length, 100 μm in width and 30 μm in depth) and a sample separation capillary 22 (20.5 mm in length, 100 μm in width and 100 μm in depth) 30 μm) intersects at an angle of 45 °, is at a distance of 1.25 mm from the buffer solution injection or storage hole 22 a located at the inner diameter side end of the sample separation capillary 22, and has a line width center of 50 μm. The cross section was formed at a distance of 2.25 mm from the sample injection port 21a located on the inner diameter side end of the sample transfer capillary 21 at the center of the line width of 50 μm. At both ends of each groove, a sample injection port (21a, 21b) having a diameter of 1.5 mm and a depth of 30 μm, and a hole (22a, 22b) for buffer solution injection or storage were formed.
[0066]
b) Then, as shown in FIGS. 3 and 4, a polycarbonate resin having the same shape as the concave portion is formed so as to completely cover the concave portion 31 in which each of the grooves, the sample injection port, and the buffer solution injection or storage hole is formed. Was bonded to the substrate 11 using an ultraviolet curing adhesive. In the capillary cover 33, a through hole having a diameter of 1.5 mm is formed at a position corresponding to a sample injection port provided in the concave portion of the substrate 11 and a hole for buffer solution injection or storage, respectively. The hole for sample injection, buffer solution injection or storage is open when viewed from above, but the capillary forming part is covered with a capillary cover to form a sealed tubular capillary with a width of 100 μm and a depth of 30 μm. are doing.
[0067]
c) Platinum sputtering is performed on the sample injection port, buffer solution injection or storage hole (a region corresponding to the hole diameter of 1.5 mm) which is open through the through hole of the capillary cover when viewed from above, to form an electrode. Formed.
[0068]
d) A fluorescent dye-containing methylcellulose solution (methylcellulose concentration 0.5%) was prepared as a buffer for electrophoresis. Specifically, 0.5 g of methylcellulose was added to 80 mL of hot purified water, stirred well, cooled in a refrigerator overnight to completely dissolve, and then tris (for biochemistry) and boric acid (special reagent grade) were added. The final concentration was adjusted to 50 mM (pH 7.5 to 8.5), and the total volume was adjusted to 100 mL with a volumetric flask. Finally, the fluorescent dye TOTO-3 was adjusted to 0.1 μg / mL. added.
[0069]
Then, an appropriate amount of the buffer solution was dropped by a micropipette into the sample inlet (21b) of the sample transfer capillary in the direction opposite to the disk, and the inside of the capillaries crossed in a cross shape by capillary action was completely filled with the buffer solution. . The capillary can be filled with the same capillary effect by capillary action regardless of where the sample injection port, buffer solution injection or storage hole is used.
[0070]
e) As a sample, a DNA standard molecular weight sample (100 bp dsDNA ladder (Gensura Laboratories. Inc., 100 g / mL)) was diluted with ultrapure water (Millipore) at the time of measurement and adjusted to 1 μg / mL. The 100 bp dsDNA ladder is a mixture of 10 (100, 200, 300, 400, 500, 600, 700, 800, 900, 1000 bp) double-stranded DNA fragments (the same applies hereinafter).
[0071]
f) 10 μl of the DNA standard molecular weight sample is dropped by a micropipette into the sample inlet (21a) in the disk rotation direction of the capillary for transferring the sample of the capillary filled with the buffer solution, and is formed in the sample inlet (21b). The ground electrode is dropped to the ground, a positive voltage of 500 volts is applied to the electrode formed in the sample inlet (21a), and when an appropriate amount reaches the intersection of the cross-shaped capillary by electrophoresis, the sample inlet (21b ), The ground contact of the electrodes and the application of the positive voltage to the electrodes of the sample inlet (21a) were released to bring them into a floating state.
[0072]
Then, the disc-shaped optical recording medium was separated and detected using a sample analyzer basically having the configuration shown in FIG.
[0073]
Specifically, the laser light source 51 and the lens system unit (52 to 55) are moved and fixed to a position 2 mm from the end of the capillary for sample separation on the side of the buffer solution storage hole (22b), and are stationary. The disk-shaped optical recording medium was rotated at a rotation speed of 300 rpm, and the DNA fragments separated and electrophoresed by centrifugal force were irradiated with laser light (wavelength 635 nm) from the laser light source fixed at the predetermined position. . The DNA fragment stained with the fluorescent dye in the buffer emits fluorescence when excited by the laser beam. The fluorescence is measured by the CCD camera 61, and the luminescence start time and the luminescence intensity of each DNA fragment from the rotation of the disk are measured. Are recorded as a pair of information in the SRAM which is a temporary recording memory, and the information on the emission intensity and the migration time of each DNA fragment temporarily recorded in the SRAM is switched to the CD-R light recording mode of the sample analyzer. As a result, printing was performed on the optical recording area of the disk-shaped optical recording medium by laser light (wavelength 780 nm). The result is shown in FIG. The vertical axis in FIG. 11 represents the emission intensity, and the horizontal axis represents the migration time (seconds) until the DNA fragment reaches the laser light source fixed position and emits light (the same applies to FIGS. 12 to 14).
[0074]
From FIG. 11, it can be seen that the DNA standard molecular weight sample is clearly separated and detected for each molecular weight, and good separation and detection ability can be obtained.
[0075]
Example 2
DNA fragments were separated and detected using the disk-shaped optical recording medium having the structure shown in FIG.
[0076]
a) For forming a capillary in an analysis area (an optical recording layer-free area) of a polycarbonate substrate having an information recording section (4.8 GB single-sided recording) similar to that of a DVD-R formed in a predetermined area. A groove 32 was formed. Specifically, as shown in FIG. 7, a sample transfer capillary (length 3.5 mm, width 80 μm, depth 40 μm) and a sample separation capillary (length 20.5 mm, width 80 μm, depth 40 μm) Intersect at an angle of 45 degrees, at a position 1.25 mm from the inner end of the disk for sample separation, at a line width center of 40 μm, and in the disk rotation direction of the sample transfer capillary (laser light source). It was formed at a position 2.25 mm from the end on the clockwise side (as viewed from above the installation site) and at the center of the line width of 40 μm as an intersection. At both ends of each capillary, sample injection ports (21′a, 21′b) having a diameter of 1.5 mm and a depth of 40 μm, and holes (22′a, 22′b) for buffer injection or storage are provided. Formed.
[0077]
b) Then, as shown in FIG. 5, the upper surface single-sided disk 34 made of polycarbonate corresponding to the capillary cover of the first embodiment is covered with the above-mentioned grooves, the sample injection port, and the buffer so as to cover the above-mentioned grooves. It adhered to the surface in which the hole for liquid injection or storage was formed using an ultraviolet curing adhesive. On the upper surface one-sided disk 34, through holes having a diameter of 1.5 mm are formed at positions corresponding to the sample injection port provided on the substrate 11 and the hole for buffer solution injection or storage, respectively. The sample inlet, buffer solution injection or storage hole is open when viewed from above, but the capillary forming part is covered with a capillary cover to form a sealed 80 μm wide, 40 μm deep tubular capillary. I have.
[0078]
c) Platinum sputtering is performed on the sample injection port, buffer solution injection or storage hole (a region corresponding to the hole diameter of 1.5 mm) which is open when viewed from above through the through hole of the upper surface one-sided disk, and the electrode is formed. Was formed.
[0079]
d) A fluorescent dye-containing methylcellulose solution (methylcellulose concentration: 0.1, 0.3, 0.5, 0.7, 1.0%) was prepared as in Example 1 as a buffer for electrophoresis. In addition, 0.05 μg / mL of TOTO-3 was added as a fluorescent dye.
[0080]
Then, an appropriate amount of the buffer solution is dropped by a micropipette into the sample inlet (21'b) of the sample transfer capillary in the direction opposite to the disk, and the inside of the capillaries crossed in a cross shape by capillary action is entirely filled with the buffer solution. Filled. The capillary can be filled with the same capillary effect by capillary action regardless of where the sample injection port, buffer solution injection or storage hole is used.
[0081]
e) As a sample, a DNA standard molecular weight sample (100 bp dsDNA ladder (Gensura Laboratories. Inc., 100 g / mL)) was diluted with ultrapure water (Millipore) at the time of measurement and adjusted to 1 μg / mL.
[0082]
f) 10 μl of the DNA standard molecular weight sample was dropped by a micropipette onto the sample inlet (21′a) in the disk rotation direction of the capillary for transferring the sample of the capillary filled with the buffer solution, and the disk-shaped optical recording medium was removed. The sample components were separated and detected using a sample analyzer basically having the configuration shown in FIG.
[0083]
Specifically, the stationary disk was rotated at a rotation speed of 500 rpm, and the DNA standard molecular weight sample was allowed to reach the intersection of the cross-shaped capillary by centrifugal force. The electrode formed in the hole (22'b) was grounded, and a positive voltage of 750 volts was applied to the electrode formed in the buffer solution inlet (22'a) to perform electrophoretic separation of DNA fragments.
[0084]
The laser light source 51 and the lens system unit (52 to 55) are moved and fixed at a position 2 mm from the buffer solution storage hole (22'b) side end of the sample separation capillary, and are electrophoresed. The irradiated DNA fragment is irradiated with laser light (wavelength 635 nm) from the laser light source fixed at the above-mentioned predetermined position, detected in the same manner as in Example 1, and the information is switched to the DVD-R optical recording mode. Burning recording was performed on the optical recording area of the disk-shaped optical recording medium using a laser beam (wavelength: 635 nm).
[0085]
Separation and detection of sample components were carried out under the same conditions using buffers having different methylcellulose concentrations. The results are shown in FIG. In FIG. 12, (1) is a methylcellulose concentration of 1.0%, (2) is a methylcellulose concentration of 0.7%, (3) is a methylcellulose concentration of 0.5%, (4) is a methylcellulose concentration of 0.3%, and (5). ▼ shows the results when a buffer having a methylcellulose concentration of 0.1% was used.
[0086]
From FIG. 12, it can be seen that good separation / detection ability can be obtained although the separation ability varies depending on the methylcellulose concentration.
[0087]
Example 3
DNA fragments were separated and detected using the disk-shaped optical recording medium having the structure shown in FIG.
[0088]
a) For forming a capillary in an analysis area (an optical recording layer-free area) of a polycarbonate substrate having an information recording section (4.8 GB single-sided recording) similar to that of a DVD-R formed in a predetermined area. A groove 32 was formed. Specifically, as shown in FIG. 2, a sample transfer capillary (length 3.5 mm, width 50 μm, depth 50 μm) and a sample separation capillary (length 20.5 mm, width 50 μm, depth 50 μm) Crosses at an angle of 45 degrees, is at a position 1.25 mm from the inner end of the disk for sample separation, at the center of the line width of 25 μm, and the end of the capillary for sample transfer on the inner diameter of the disk. It was formed so as to be located at a position of 2.25 mm from the center and a point of intersection of 25 μm at the center of the line width. At both ends of each capillary, a sample inlet (21a, 21b) having a diameter of 1.5 mm and a depth of 50 μm, and a hole (22a, 22b) for buffer solution injection or storage were formed.
[0089]
b) Then, as shown in FIG. 5, the upper surface single-sided disk 34 made of polycarbonate corresponding to the capillary cover of the first embodiment is covered with the above-mentioned grooves, the sample injection port, and the buffer so as to cover the above-mentioned grooves. It adhered to the surface in which the hole for liquid injection or storage was formed using an ultraviolet curing adhesive. On the upper surface one-sided disk 34, through holes having a diameter of 1.5 mm are formed at positions corresponding to the sample injection port provided on the substrate 11 and the hole for buffer solution injection or storage, respectively. The sample inlet, buffer injection or storage hole is open when viewed from above, but the capillary forming part is covered with a capillary cover to form a sealed 50 μm wide, 50 μm deep tubular capillary. I have.
[0090]
c) Platinum sputtering is performed on the sample injection port, buffer solution injection or storage hole (a region corresponding to the hole diameter of 1.5 mm) which is open when viewed from above through the through hole of the upper surface one-sided disk, and the electrode is formed. Was formed.
[0091]
d) A 0.5% methylcellulose solution containing a fluorescent dye (TOTO-3) was prepared in the same manner as in Example 1 as a buffer for electrophoresis.
[0092]
Then, an appropriate amount of the buffer solution was dropped by a micropipette into the sample inlet (21b) of the sample transfer capillary in the direction opposite to the disk, and the inside of the capillaries crossed in a cross shape by capillary action was completely filled with the buffer solution. .
[0093]
e) A microbial gene amplified product (500 bp) obtained by amplifying a 500 bp fragment by PCR (polymerase chain reaction) using phage DNA as a template was used as a sample. As primers for PCR, those shown in SEQ ID NO: 1 (primer on the 5 ′ side) and SEQ ID NO: 2 (primer on the 3 ′ side) were used. The PCR reaction solution had a final volume of 50 μL, and 0.5 ng of template DNA was added to each of the two primers at 1.0 μmol / L, 200 mmol / L potassium chloride (KCl), and 15 mmol / L magnesium chloride (MgCl). ₂ ) And 0.01% (mass / volume ratio) gelatin. In the PCR, using a device manufactured by PERKIN ELMER, a thermal denaturation reaction was performed at 94 ° C. for 15 seconds, and an annealing and extension reaction was performed at 68 ° C. for 30 seconds, and this was repeated 25 cycles.
"Sequence List Free Text"
SEQ ID NO: 1: PCR primer
SEQ ID NO: 2: Primer for PCR
[0094]
f) The microbial gene amplification product is dropped by 10 μl to the sample inlet (21a) in the disk rotation direction of the capillary for transferring the sample of the capillary filled with the buffer solution, and formed into the sample inlet (21b). The ground electrode is dropped to the ground, a positive voltage of 500 volts is applied to the electrode formed in the sample inlet (21a), and when an appropriate amount reaches the intersection of the cross-shaped capillary by electrophoresis, the sample inlet (21b ), The ground contact of the electrodes and the application of the positive voltage to the electrodes of the sample inlet (21a) were released to bring them into a floating state.
[0095]
Then, the electrode formed in the storage hole (22b) is brought into ground contact, and a positive voltage of 800 volts is applied to the electrode formed in the buffer inlet (22a) to perform electrophoretic separation of the microorganism gene amplification product. In the same manner as in Example 2, detection of sample components and recording of information were performed. The result is shown in FIG.
[0096]
FIG. 13 shows that the DNA fragment amplified by PCR can be accurately separated and detected.
[0097]
Example 4
Using the same disk-shaped optical recording medium as in Example 1, using a 0.7% hydroxypropylmethylcellulose solution containing a fluorescent dye (TOTO-3) as a buffer during electrophoresis, a DNA standard molecular weight sample 100 bp dsDNA ladder (Gensura) Laboratories, Inc., 100 g / mL).
[0098]
Specifically, 10 μl of the DNA standard molecular weight sample was dropped with a micropipette onto the sample inlet (21a) in the disk rotation direction of the capillary for transferring the sample of the capillary filled with the buffer solution, and the sample inlet (21b) Is grounded, a positive voltage of 600 volts is applied to the electrode formed at the sample inlet (21a), and when an appropriate amount reaches the crossing point of the cross-shaped capillary by electrophoresis, the sample is injected. The ground contact of the electrode at the inlet (21b) and the application of the positive voltage to the electrode at the sample inlet (21a) were released to bring the electrode into a floating state.
[0099]
Then, the sample components were separated and detected in the same manner as in Example 1 except that the rotation speed of the disc-shaped optical recording medium was set to 200 rpm, and the information was recorded in the optical recording area of the disc-shaped optical recording medium. FIG. 14 shows the result.
[0100]
From FIG. 14, it can be seen that the DNA standard molecular weight sample is clearly separated and detected for each molecular weight, and good separation and detection ability can be obtained.
[0101]
Example 5
When the same disk-shaped optical recording medium as in Example 1 was used to perform the separation and migration detection of the same sample (DNA standard molecular weight sample 100 bp dsDNA ladder, Gensura Laboratories. Inc., 100 g / mL) in the same manner as in Example 1 In the recording area of the optical recording medium, the following items of the sample, various items of the sample movement / migration separation detection conditions, and a program for executing the same were recorded in advance.
[0102]
・ Sample items
1. Sample name: standard molecular weight sample 100 bp dsDNA ladder, 100 g / mL
2. Sample manufacturer: Gensura Laboratories Inc.
3. Sample concentration after dilution with ultrapure water: 1 μg / mL
・ Electrophoretic separation detection conditions
1. Concentration of methylcellulose solution: 0.5%
2. Final concentration of Tris and boric acid: 50 mM
3. Type of fluorescent dye: TOTO-3
4. Addition amount of fluorescent dye: 0.1 μg / ml
5. Drop volume of sample after ultra-pure dilution into sample injection hole: 10 μL
6. Applied voltage between sample injection hole electrode and ground electrode: 500 volts
7. Time required for the appropriate amount of sample to reach the cross-shaped capillary intersection by electrophoresis: 150 seconds
8. Time from application voltage ON to OFF (floating state) for moving an appropriate amount of sample: 150 seconds
9. Start of disk rotation for electrophoretic separation detection: 1 second after floating state (applied voltage OFF)
10. Movement of detection position of laser light source and lens system unit and laser light source ON: Floating state of 9 (applied voltage OFF) Move to position 2 mm from end of buffer solution storage hole, and turn on laser after movement
11. Start of disk rotation: 1 second after 10 lasers ON
12. Disk rotation speed: 300 rpm
13. CCD camera ON, sensing start: Simultaneous with 10 laser ON
14． Start writing fluorescence emission sensing data (emission intensity-time) to SRAM: disk rotation ON
15. Laser OFF, rotation OFF, CCD camera OFF: 3 minutes after the emission intensity detection is judged to be the noise level
16. Burning of data recorded in SRAM: 15 milliseconds after laser off, rotation off, CCD camera off
[0103]
In carrying out the sample analysis work, the sample preparation and operating procedure were exactly the same as in Example 1, and after the sample was dropped into the sample injection hole, the sample analyzer was automatically operated according to a predetermined program. 1 was written to the optical recording medium, and it was confirmed that the result could be reproduced by the sample analyzer.
[0104]
Before dropping the sample into the sample injection hole, confirming the prerecorded items of the sample and the various items of the electrophoretic separation detection conditions are based on the data of the optical recording medium of the sample analyzer shown in FIG. It can be easily confirmed by the reading function.
[0105]
【The invention's effect】
As described above, the disc-shaped optical recording medium of the present invention performs the separation and detection of the sample by the microcapillary formed in the analysis region on the substrate surface, and the analysis information is formed on the disc-shaped substrate surface. Since the data can be recorded and stored in the optical recording area, the process from separation and detection of the sample to recording of the analysis information can be performed on one disk.
[0106]
Further, by using the sample analyzer of the present invention, it is possible to easily detect the sample components separated by the microcapillary on the disk-shaped optical recording medium and record the analysis information thereof.
[0107]
[Sequence list]

[Brief description of the drawings]
FIG. 1 is a plan view of a disk-shaped optical recording medium having a microcapillary according to an embodiment of the present invention.
FIG. 2 is an enlarged schematic view of a microcapillary formed on the surface of the disk-shaped optical recording medium.
FIG. 3 is a schematic sectional view of the disk-shaped optical recording medium.
4 is an enlarged view of a boundary portion between an optical recording area and an analysis area in FIG.
FIG. 5 is a diagram showing an example in which an upper surface single-sided disk having no laminated recording layer is used as a substitute for the capillary cover.
FIG. 6 is a plan view of a disc-shaped optical recording medium having a microcapillary according to another embodiment of the present invention.
FIG. 7 is an enlarged schematic view of a microcapillary formed on the surface of the disk-shaped optical recording medium.
FIG. 8 is a plan view of a disc-shaped optical recording medium having a microcapillary according to still another embodiment of the present invention.
FIG. 9 is an enlarged partial plan view showing an analysis area formed on the disc-shaped optical recording medium.
FIG. 10 is a schematic configuration diagram showing one embodiment of a sample analyzer of the present invention.
FIG. 11 is a diagram showing the results of detection by electrophoresis of a DNA standard molecular weight sample using the disk-shaped optical recording medium and the sample analyzer of the present invention in Example 1.
FIG. 12 is a diagram showing the results of detection by electrophoresis of a DNA standard molecular weight sample using the disk-shaped optical recording medium and the sample analyzer of the present invention in Example 2.
FIG. 13 is a diagram showing the results of separating and detecting microbial gene amplification products by PCR using the disk-shaped optical recording medium and the sample analyzer of the present invention in Example 3.
FIG. 14 is a diagram showing the results of separation and detection of a DNA standard molecular weight sample using the disk-shaped optical recording medium and the sample analyzer of the present invention in Example 4.
[Explanation of symbols]
1, 2, 3,. Disc-shaped optical recording medium having microcapillaries
11. substrate
12. Center hole
13. Disk holder
14． Analysis area
15. Optical recording area
21, 24. Capillary for sample transfer
21a, 21b, 24a. Sample inlet
22, 25. Capillary for sample separation
23, 26. Microcapillary
27, 28. electrode
31. Recess
32. groove
33. Capillary cover
34. Top one side disk
41. Guide groove
42. Recording layer
43. Reflective layer
44. Protective film
51. Laser light source
52. Multi lens
53. Beam splitter
54. Collimator
55. Objective lens
56. Condenser lens
57. Photodetector
58. Amplifier circuit
59. Servo / signal processing LSI
60. Laser drive circuit
61. CCD camera

Claims (9)

A disk-shaped substrate, an optical recording area formed on the surface of the substrate, and an analysis area, wherein the analysis area is formed with a microcapillary having a sample injection port at least at one end. Disc-shaped optical recording medium having a micro-capillary to be formed. The microcapillary is formed radially with respect to the center of the substrate, the sample injection port is formed at an end on the center side of the substrate, and the sample is injected by centrifugal force when the substrate is rotated. The disk-shaped optical recording medium having a microcapillary according to claim 1, which separates components. 2. The microcapillary has electrodes formed at least at two points in a path of the microcapillary, and separates the sample components by applying a voltage between the electrodes to cause electrophoresis of the sample components. Or a disk-shaped optical recording medium having the microcapillary according to 2. The microcapillary comprises a sample transfer capillary and a sample separation capillary, and the sample transfer capillary has a sample inlet at least at one end thereof, and intersects with the sample separation capillary in the middle of a path. The disk-shaped optical recording medium having a microcapillary according to any one of claims 1 to 3, which is connected to the sample separation capillary at the other end thereof. 5. The disk-shaped optical recording medium according to claim 4, wherein holes for buffer solution injection or storage are formed at both ends of the sample separation capillary. The disk-shaped optical recording device having a microcapillary according to claim 1, wherein the microcapillary includes a groove formed on the surface of the substrate and a cover closing an opening of the groove. Medium. The disk-shaped optical recording medium having a microcapillary according to claim 1, wherein the optical recording region and the analysis region are formed on the same surface of the disk-shaped substrate. The disk-shaped optical recording medium having a microcapillary according to claim 1, wherein the optical recording layer formed in the optical recording area is a write-once recording layer. A disc-shaped optical recording medium having the microcapillary according to any one of claims 1 to 8, a turntable for supporting and rotating the disc-shaped optical recording medium, and applying a laser beam to the disc-shaped optical recording medium. A laser beam irradiating unit for irradiating, a laser beam receiving unit for receiving a reflected light of the laser beam radiated to the optical recording region of the optical recording medium, and A sample analyzer, comprising: image detection means for detecting an image to be formed.

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