JP2003516140A - 微生物の流加培養によるペプチドの生産 - Google Patents

微生物の流加培養によるペプチドの生産

Info

Publication number: JP2003516140A
Authority: JP; Japan
Prior art keywords: glucose; carbon source; fed; organic carbon; rhgh
Prior art date: 1999-12-09
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

JP2001544297A

Other languages

English (en)

Japanese (ja)

Inventor

カスタン，アンドレアス

Original Assignee

フアーマシア・アー・ベー

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-12-09

Filing date

2000-11-29

Publication date

2003-05-13

2000-11-29 Application filed by フアーマシア・アー・ベー filed Critical フアーマシア・アー・ベー

2003-05-13 Publication of JP2003516140A publication Critical patent/JP2003516140A/ja

Status Withdrawn legal-status Critical Current

Links

238000004519 manufacturing process Methods 0.000 title claims abstract description 13
108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 10
244000005700 microbiome Species 0.000 title claims abstract description 7
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 33
239000008103 glucose Substances 0.000 claims abstract description 33
238000000034 method Methods 0.000 claims abstract description 25
229910052799 carbon Inorganic materials 0.000 claims abstract description 21
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 20
238000003756 stirring Methods 0.000 claims abstract description 10
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 8
102000018997 Growth Hormone Human genes 0.000 claims abstract description 4
108010051696 Growth Hormone Proteins 0.000 claims abstract description 4
239000000122 growth hormone Substances 0.000 claims abstract description 4
229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 4
229910052757 nitrogen Inorganic materials 0.000 claims abstract description 4
230000003534 oscillatory effect Effects 0.000 claims description 13
241000588724 Escherichia coli Species 0.000 claims description 7
102000002265 Human Growth Hormone Human genes 0.000 claims description 5
108010000521 Human Growth Hormone Proteins 0.000 claims description 5
239000000854 Human Growth Hormone Substances 0.000 claims description 5
125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 abstract description 4
102000004196 processed proteins & peptides Human genes 0.000 abstract description 3
210000004027 cell Anatomy 0.000 description 9
239000000758 substrate Substances 0.000 description 7
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
235000003642 hunger Nutrition 0.000 description 6
239000002609 medium Substances 0.000 description 6
229910052760 oxygen Inorganic materials 0.000 description 6
239000001301 oxygen Substances 0.000 description 6
230000037351 starvation Effects 0.000 description 6
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 5
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 5
108090000623 proteins and genes Proteins 0.000 description 5
102000004169 proteins and genes Human genes 0.000 description 5
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
238000012258 culturing Methods 0.000 description 4
230000004060 metabolic process Effects 0.000 description 4
230000010355 oscillation Effects 0.000 description 4
239000013612 plasmid Substances 0.000 description 4
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239000002028 Biomass Substances 0.000 description 3
238000013019 agitation Methods 0.000 description 3
238000006243 chemical reaction Methods 0.000 description 3
238000005516 engineering process Methods 0.000 description 3
238000004191 hydrophobic interaction chromatography Methods 0.000 description 3
230000001965 increasing effect Effects 0.000 description 3
238000012546 transfer Methods 0.000 description 3
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
108020004511 Recombinant DNA Proteins 0.000 description 2
240000004808 Saccharomyces cerevisiae Species 0.000 description 2
235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
102000003978 Tissue Plasminogen Activator Human genes 0.000 description 2
108090000373 Tissue Plasminogen Activator Proteins 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
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239000000463 material Substances 0.000 description 2
239000012533 medium component Substances 0.000 description 2
230000002829 reductive effect Effects 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
239000000243 solution Substances 0.000 description 2
229960000187 tissue plasminogen activator Drugs 0.000 description 2
230000014616 translation Effects 0.000 description 2
230000005428 wave function Effects 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
238000012366 Fed-batch cultivation Methods 0.000 description 1
101000976075 Homo sapiens Insulin Proteins 0.000 description 1
102000003839 Human Proteins Human genes 0.000 description 1
108090000144 Human Proteins Proteins 0.000 description 1
102000006992 Interferon-alpha Human genes 0.000 description 1
108010047761 Interferon-alpha Proteins 0.000 description 1
102000008070 Interferon-gamma Human genes 0.000 description 1
108010074328 Interferon-gamma Proteins 0.000 description 1
102100021941 Sorcin Human genes 0.000 description 1
101710089292 Sorcin Proteins 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
239000000654 additive Substances 0.000 description 1
230000000996 additive effect Effects 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
229910021529 ammonia Inorganic materials 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
230000008901 benefit Effects 0.000 description 1
108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
238000004113 cell culture Methods 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
239000000306 component Substances 0.000 description 1
238000012790 confirmation Methods 0.000 description 1
238000012136 culture method Methods 0.000 description 1
125000004122 cyclic group Chemical group 0.000 description 1
230000001086 cytosolic effect Effects 0.000 description 1
230000007423 decrease Effects 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
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230000007613 environmental effect Effects 0.000 description 1
239000011536 extraction buffer Substances 0.000 description 1
239000006260 foam Substances 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
101150091570 gapA gene Proteins 0.000 description 1
230000004190 glucose uptake Effects 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
229910052500 inorganic mineral Inorganic materials 0.000 description 1
PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 description 1
229950000038 interferon alfa Drugs 0.000 description 1
239000001573 invertase Substances 0.000 description 1
235000011073 invertase Nutrition 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
239000002207 metabolite Substances 0.000 description 1
239000011707 mineral Substances 0.000 description 1
235000010755 mineral Nutrition 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000005457 optimization Methods 0.000 description 1
230000036284 oxygen consumption Effects 0.000 description 1
239000008188 pellet Substances 0.000 description 1
210000001322 periplasm Anatomy 0.000 description 1
229910052698 phosphorus Inorganic materials 0.000 description 1
230000008092 positive effect Effects 0.000 description 1
230000017854 proteolysis Effects 0.000 description 1
238000000746 purification Methods 0.000 description 1
230000003252 repetitive effect Effects 0.000 description 1
230000004044 response Effects 0.000 description 1
238000007790 scraping Methods 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
229910052717 sulfur Inorganic materials 0.000 description 1
238000004448 titration Methods 0.000 description 1
235000013619 trace mineral Nutrition 0.000 description 1
239000011573 trace mineral Substances 0.000 description 1
238000012549 training Methods 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
210000005253 yeast cell Anatomy 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
- C07K14/615—Extraction from natural sources

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Engineering & Computer Science (AREA)
Endocrinology (AREA)
Microbiology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Biophysics (AREA)
Toxicology (AREA)
Medicinal Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

JP2001544297A 1999-12-09 2000-11-29 微生物の流加培養によるペプチドの生産 Withdrawn JP2003516140A (ja)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
SE9904502-3		1999-12-09
SE9904502A SE9904502D0 (sv)	1999-12-09	1999-12-09	Production of peptides
PCT/SE2000/002373 WO2001042420A1 (en)	1999-12-09	2000-11-29	Production of peptides by fedbatch cultivation of a microorganism

Publications (1)

Publication Number	Publication Date
JP2003516140A true JP2003516140A (ja)	2003-05-13

Family

ID=20418057

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP2001544297A Withdrawn JP2003516140A (ja)	1999-12-09	2000-11-29	微生物の流加培養によるペプチドの生産

Country Status (10)

Country	Link
EP (1)	EP1244769A1 (sv)
JP (1)	JP2003516140A (sv)
KR (1)	KR20020060258A (sv)
CN (1)	CN1267562C (sv)
AU (1)	AU780122B2 (sv)
CA (1)	CA2392268A1 (sv)
IL (1)	IL149624A0 (sv)
NZ (1)	NZ518919A (sv)
SE (1)	SE9904502D0 (sv)
WO (1)	WO2001042420A1 (sv)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2013541946A (ja) *	2010-09-21	2013-11-21	フェリングベスローテンフェンノートシャップ	組換えヒト成長ホルモン産生の改善された方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SI1545428T1 (sl) *	2002-09-20	2010-03-31	Pharmacia Corp	Postopek zniževanja agregatnih nivojev pegiliranega proteina
PA8588901A1 (es) *	2002-11-20	2005-02-04	Pharmacia Corp	Conjugados de hormona de crecimiento humana pegilados n-terminales y proceso para su preparacion
US20100047870A1 (en) *	2004-12-21	2010-02-25	Usv Limited	Low cell density fermentation process for the production of heterologous recombinant proteins in microorganisms
WO2008010005A1 (en) *	2006-07-14	2008-01-24	Abb Research Ltd	A method for on-line optimization of a fed-batch fermentation unit to maximize the product yield.
CN106222309A (zh) *	2016-07-28	2016-12-14	山东金朗生物科技有限公司	一种微生物发酵生产提高l‑丙氨酸产量的补料控制方法
CN108624516B (zh) *	2017-03-20	2022-08-26	华东理工大学	一种提高发酵细胞中的代谢产物量及制备idms标准品的方法
GB202010934D0 (en) *	2020-07-15	2020-08-26	Ipsen Biopharm Ltd	Controlling operation of a bioreactor vessel

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DD271850A1 (de) *	1988-05-12	1989-09-20	Adw Ddr	Mischvorrichtung zur fluidverteilung und/oder zum gaseintrag in biologischen kultursuspensionen mit pendelruehrer
BE1008008A3 (fr) *	1990-11-30	1995-12-12	Ajinomoto Kk	Procede et appareil pour regler la concentration en source de carbone dans la culture aerobie d'un micro-organisme.

1999
- 1999-12-09 SE SE9904502A patent/SE9904502D0/sv unknown
2000
- 2000-11-29 CA CA002392268A patent/CA2392268A1/en not_active Abandoned
- 2000-11-29 CN CNB008168865A patent/CN1267562C/zh not_active Expired - Fee Related
- 2000-11-29 EP EP00982029A patent/EP1244769A1/en not_active Withdrawn
- 2000-11-29 JP JP2001544297A patent/JP2003516140A/ja not_active Withdrawn
- 2000-11-29 IL IL14962400A patent/IL149624A0/xx unknown
- 2000-11-29 WO PCT/SE2000/002373 patent/WO2001042420A1/en active IP Right Grant
- 2000-11-29 KR KR1020027007309A patent/KR20020060258A/ko not_active Ceased
- 2000-11-29 AU AU19105/01A patent/AU780122B2/en not_active Ceased
- 2000-11-29 NZ NZ518919A patent/NZ518919A/en unknown

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2013541946A (ja) *	2010-09-21	2013-11-21	フェリングベスローテンフェンノートシャップ	組換えヒト成長ホルモン産生の改善された方法

Also Published As

Publication number	Publication date
CN1267562C (zh)	2006-08-02
HK1053326A1 (en)	2003-10-17
AU1910501A (en)	2001-06-18
AU780122B2 (en)	2005-03-03
NZ518919A (en)	2003-10-31
CA2392268A1 (en)	2001-06-14
EP1244769A1 (en)	2002-10-02
WO2001042420A1 (en)	2001-06-14
KR20020060258A (ko)	2002-07-16
SE9904502D0 (sv)	1999-12-09
CN1409755A (zh)	2003-04-09
IL149624A0 (en)	2002-11-10

Publication	Publication Date	Title
US6680181B2 (en)	2004-01-20	Production of peptides
Tripathi et al.	2009	High yield production of heterologous proteins with Escherichia coli
Wong et al.	1998	Effect of post‐induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli
Song et al.	2017	High purity recombinant human growth hormone (rhGH) expression in Escherichia coli under phoA promoter
JP2003516140A (ja)	2003-05-13	微生物の流加培養によるペプチドの生産
WO2010062279A1 (ru)	2010-06-03	Способ получения рекомбинантного инсулина человека
JP2006507821A (ja)	2006-03-09	物質を生産するために細胞を培養する方法
EP0219791B1 (en)	1993-04-21	Process for culturing recombinant cells
US20110189733A1 (en)	2011-08-04	Low cell density fermentation process for the production of heterologous recombinant proteins in microorganisms
KR900007630B1 (ko)	1990-10-17	아세트산을 지표로한 배양방법 및 그 장치
US5955323A (en)	1999-09-21	Automated high-yield fermentation of plasmid DNA in Escherichia coli
AU775301B2 (en)	2004-07-29	Method for increasing the yield of recombinant proteins in microbial fermentation processes
Lee et al.	1997	Mass production of thermostable D‐hydantoinase by batch culture of recombinant Escherichia coli with a constitutive expression system
Pérez-Sánchez et al.	2011	High-level production of a recombinant Vibrio proteolyticus leucine aminopeptidase and its use for N-terminal methionine excision from interferon alpha-2b
RU2697375C2 (ru)	2019-08-13	ПЛАЗМИДНЫЙ ВЕКТОР pRh15A ДЛЯ ПОЛУЧЕНИЯ БЕЗМЕТИОНИНОВОГО ИНТЕРФЕРОНА АЛЬФА-2b, ШТАММ БАКТЕРИЙ ESCHERICHIA COLI BL21 DE3 - ПРОДУЦЕНТ БЕЗМЕТИОНИНОВОГО ИНТЕРФЕРОНА АЛЬФА-2b И СПОСОБ ПОЛУЧЕНИЯ БЕЗМЕТИОНИНОВОГО ИНТЕРФЕРОНА АЛЬФА-2b
Narciandi et al.	1992	Production of the recombinant p36 protein from HIV-2 in Escherichia coli using the trp promoter
HK1053326B (en)	2006-11-24	Production of peptides by fedbatch cultivation of a microorganism
US20060286631A1 (en)	2006-12-21	Method for preparating t-20 peptide by high cell density cultivation of recombinant e. coli containing t-20 peptide coding gene
Zomorrodipour et al.	2004	The over-expression of biologically active human growth hormone in a T5-based system in Escherichia coli, studying temperature effect
Narciandi et al.	1996	Maximizing the expression of recombinant Kringle 1 (Streptokinase) synthesized in Escherichia coli. Influence of culture and induction conditions
CN116323960A (zh)	2023-06-23	使用部分收获的芽孢杆菌工业发酵工艺
Muth	1991	Drug Manufacture
CN118373922A (zh)	2024-07-23	可溶性的标签蛋白在制备司美格鲁肽主链中的应用
JPH0455671B2 (sv)	1992-09-04
MXPA97005335A (en)	1999-02-24	Process in two stages for the production of cells containing matured protein with biological activity

Legal Events

Date	Code	Title	Description
2008-02-05	A300	Application deemed to be withdrawn because no request for examination was validly filed	Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20080205

Date

Code

Title

Description

2008-02-05

A300

Application deemed to be withdrawn because no request for examination was validly filed

Free format text: JAPANESE INTERMEDIATE CODE: A300

Effective date: 20080205

JP2003516140A - 微生物の流加培養によるペプチドの生産 - Google Patents

Info

Links

Classifications

Landscapes

Applications Claiming Priority (3)

Publications (1)

Family

ID=20418057

Family Applications (1)

Country Status (10)

Cited By (1)

Families Citing this family (7)

Family Cites Families (2)

Cited By (1)

Also Published As

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