JP2003262588A5 - - Google Patents
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- JP2003262588A5 JP2003262588A5 JP2002341244A JP2002341244A JP2003262588A5 JP 2003262588 A5 JP2003262588 A5 JP 2003262588A5 JP 2002341244 A JP2002341244 A JP 2002341244A JP 2002341244 A JP2002341244 A JP 2002341244A JP 2003262588 A5 JP2003262588 A5 JP 2003262588A5
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- JP
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- Prior art keywords
- microarray
- light
- imaging
- transmitted
- fluorescent substance
- Prior art date
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- 238000002493 microarray Methods 0.000 description 20
- 239000000126 substance Substances 0.000 description 16
- 238000003384 imaging method Methods 0.000 description 13
- 230000001678 irradiating effect Effects 0.000 description 5
- 230000005284 excitation Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Description
【特許請求の範囲】
【請求項１】
蛍光物質が付与された検体を反応させた複数の区画を含む生体関連物質マイクロアレイから、蛍光物質が発する蛍光を読み取る読取装置であって、マイクロアレイに光を透過させる透過用光源と、区画に存在する蛍光物質を励起する励起用光源と、透過用光源から照射され、マイクロアレイを透過した透過光、及び励起用光源によって励起された蛍光物質から発せられる蛍光を撮像する撮像手段と、この撮像手段によって撮像された、マイクロアレイを透過した光の画像に基づいて、マイクロアレイ上に配置された区画の位置及び／又は大きさを検出する検出手段とを含む、生体関連物質マイクロアレイ読取装置。
【請求項２】
マイクロアレイを透過した光を撮像する際の光学系と、蛍光物質から発せられた蛍光を撮像する際の光学系との差を補正する補正手段を更に含む、請求項１記載の読取装置。
【請求項３】
複数の区画を含む生体関連物質マイクロアレイに、蛍光物質が付与された検体を反応させるステップと、該マイクロアレイに光を照射し、マイクロアレイを透過した透過光を撮像するステップと、前記ステップにおいて撮像された透過光に基づいて、区画の位置及び／又は大きさを検出するステップと、マイクロアレイに励起光を照射してマイクロアレイの区画に存在する蛍光物質を励起し、この蛍光物質から発せられる蛍光を撮像するステップと、前記ステップにおいて撮像された蛍光を、透過光に基づいて検出された区画の位置及び／又は大きさに基づいて読み取るステップとを含む、生体関連物質マイクロアレイの読取方法。
【請求項４】
複数の区画を含む生体関連物質マイクロアレイに、蛍光物質が付与された検体を反応させるステップと、該マイクロアレイに光を照射し、マイクロアレイを透過した透過光を撮像するステップと、前記ステップにおいて撮像された透過光に基づいて、区画の位置を、撮像する視野内の適切な位置に移動させるステップと、再びマイクロアレイに光を照射し、マイクロアレイを透過した透過光を撮像するステップと、区画の位置及び／又は大きさを検出するステップと、マイクロアレイに励起光を照射してマイクロアレイの区画に存在する蛍光物質を励起し、この蛍光物質から発せられる蛍光を撮像するステップと、前記ステップにおいて撮像された蛍光を、透過光に基づいて検出された区画の位置及び／又は大きさに基づいて読み取るステップとを含む、生体関連物質マイクロアレイの読取方法。
[Claims]
(1)
A reading device that reads fluorescence emitted from a fluorescent substance from a biologically-related substance microarray including a plurality of sections in which a specimen provided with a fluorescent substance is reacted, wherein the reading light source transmits light to the microarray, and is present in the section. An excitation light source that excites a fluorescent substance, imaging means for imaging transmitted light emitted from the transmission light source and transmitted through the microarray, and fluorescence emitted from the fluorescent substance excited by the excitation light source, and imaging by the imaging means A detection unit for detecting the position and / or size of a section arranged on the microarray based on an image of light transmitted through the microarray.
(2)
2. The reading apparatus according to claim 1, further comprising a correction unit configured to correct a difference between an optical system for imaging light transmitted through the microarray and an optical system for imaging fluorescence emitted from a fluorescent substance.
(3)
The step of reacting a specimen provided with a fluorescent substance with a biological-related substance microarray including a plurality of compartments, the step of irradiating the microarray with light, and the step of imaging transmitted light transmitted through the microarray, and the step of imaging in the step Detecting the position and / or size of the section based on the transmitted light; irradiating the microarray with excitation light to excite the fluorescent substance present in the section of the microarray, and imaging the fluorescence emitted from the fluorescent substance A method for reading a biologically relevant substance microarray, comprising: a step of reading the fluorescence imaged in the step based on the position and / or size of the section detected based on the transmitted light.
(4)
The step of reacting a specimen provided with a fluorescent substance with a biological-related substance microarray including a plurality of compartments, the step of irradiating the microarray with light, and the step of imaging transmitted light transmitted through the microarray, and the step of imaging in the step Moving the position of the section to an appropriate position in the visual field to be imaged based on the transmitted light, irradiating the microarray again with light, and imaging the transmitted light transmitted through the microarray; Or detecting the size, irradiating the microarray with excitation light to excite the fluorescent substance present in the section of the microarray, imaging fluorescent light emitted from the fluorescent substance, and converting the fluorescent light imaged in the step to Reading based on the position and / or size of the section detected based on the transmitted light. Including bets, reading method of a biological substance microarray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002341244A JP4043925B2 (en) | 2001-12-05 | 2002-11-25 | Biological material microarray reader and method |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001371485 | 2001-12-05 | ||
| JP2001-371485 | 2001-12-05 | ||
| JP2002341244A JP4043925B2 (en) | 2001-12-05 | 2002-11-25 | Biological material microarray reader and method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003262588A JP2003262588A (en) | 2003-09-19 |
| JP2003262588A5 true JP2003262588A5 (en) | 2006-01-19 |
| JP4043925B2 JP4043925B2 (en) | 2008-02-06 |
Family
ID=29217702
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002341244A Expired - Fee Related JP4043925B2 (en) | 2001-12-05 | 2002-11-25 | Biological material microarray reader and method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4043925B2 (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7428322B2 (en) * | 2004-04-20 | 2008-09-23 | Bio-Rad Laboratories, Inc. | Imaging method and apparatus |
| CA2984772C (en) | 2005-09-21 | 2018-04-03 | Luminex Corporation | Methods and systems for image data processing |
| JP4955244B2 (en) * | 2005-09-27 | 2012-06-20 | 横河電機株式会社 | Biochip reader and biochip reader method |
| JP2008020412A (en) | 2006-07-14 | 2008-01-31 | Sony Corp | Bioassay-use substrate and bioassay method |
| JP2008051773A (en) * | 2006-08-28 | 2008-03-06 | Hamamatsu Photonics Kk | Fluorescence image acquisition device and fluorescence image acquisition method |
| JP5100757B2 (en) * | 2006-11-30 | 2012-12-19 | キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド | System and method for monitoring amplification and dissociation reactions of DNA molecules |
| JP4979516B2 (en) * | 2007-08-31 | 2012-07-18 | 三菱レイヨン株式会社 | Image reading method and apparatus |
| JP2009222588A (en) * | 2008-03-17 | 2009-10-01 | Panasonic Corp | Microplate reading device and microplate reading method |
| CA2852915C (en) | 2011-10-18 | 2018-05-15 | Luminex Corporation | Methods and systems for image data processing |
| JP6239811B2 (en) * | 2012-04-26 | 2017-11-29 | 株式会社島津製作所 | Measuring program and measuring device |
| JP2014185931A (en) * | 2013-03-22 | 2014-10-02 | Shimadzu Corp | Measurement program and radioactivity measurement system |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0293346A (en) * | 1988-09-29 | 1990-04-04 | Toshiba Corp | Foreign matter analyser |
| JPH0835927A (en) * | 1994-07-25 | 1996-02-06 | Jasco Corp | Flow cell for fluorescence detection and fluorescence detector using the same |
| JP3766498B2 (en) * | 1997-01-24 | 2006-04-12 | オリンパス株式会社 | Scanning cell measuring apparatus and cell measuring method |
| JP3618511B2 (en) * | 1997-04-14 | 2005-02-09 | オリンパス株式会社 | Microchannel element |
| JP4286447B2 (en) * | 1997-08-07 | 2009-07-01 | ジーイー・ヘルスケア・ナイアガラ・インク | Digital imaging system for assays in well plates, gels and blots |
| JP3511910B2 (en) * | 1998-10-14 | 2004-03-29 | 株式会社島津製作所 | Detector cell |
| JP3978937B2 (en) * | 1999-06-08 | 2007-09-19 | 株式会社島津製作所 | Detector cell and optical measuring device |
| JP2001027607A (en) * | 1999-07-15 | 2001-01-30 | Kaken Kogyo:Kk | Observation apparatus |
| JP2001074658A (en) * | 1999-09-09 | 2001-03-23 | Fuji Photo Film Co Ltd | Photometric method and apparatus |
-
2002
- 2002-11-25 JP JP2002341244A patent/JP4043925B2/en not_active Expired - Fee Related
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