JP2003185566A - Analytical optical disc and its measuring device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a low-cost and high-accuracy optical disk for analysis by a method wherein a solution to be inspected at the optical disk for analysis is airtightly sealed so as to prevent its outflow, and to provide a measuring device for the optical disk. <P>SOLUTION: A lid 1, an analytical reagent 5 and the optical disk 9 for analysis including a substrate 7 are integrated. They are airtightly sealed with upper lids 17, the outflow of the solution to be inspected is prevented, and the optical disk for analysis which is safe and of high accuracy is provided. The measuring device which is composed of a shaft 10, a light source 11, a light receiving element 12, an operating engine 13, a motor 14 and a circuit 15, and which is of a simple constitution and low-cost, is provided. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to the structure and function of an analysis disk and a measuring instrument using an optical disk.

[0002]

2. Description of the Prior Art In the fields of analytical evaluation of medicine, biology and chemistry, mechanical and automated test solution handling systems and instruments are well known in the prior art.

In 1968, Anal. Biochem. 545 of 28
On page 562, Anderson teaches a multiple cuvette rotor for cell fractionation, which was a method of simultaneously measuring multiple solutions under test for one type of analyte. The degree of perfection was low.

In 1974, Clin. Chem 20 932-9
On page 41, Burtis et al. Teach a method of dynamically introducing a liquid into a centrifuge analyzer.

On July 21, 1981, Berzelet (Ber
US Pat. No. 4,27, issued to Taudiere) et al.
No. 9,862 discloses a centrifuge photometric analyzer, which has a test solution introducing hole and a test reagent introducing hole separately, which is far from automation and very inconvenient for the measurer.

In US Pat. No. 4,515,889 issued May 7, 1985 to Klose et al., The reagents were confused and incubated under centrifugal force to allow analytical It teaches how to perform various measurements.

On June 30, 1987, Edeman (Ede
U.S. Pat. No. 4,676,9 issued to Lman et al.
No. 52 teaches a photometric analyzer, but it takes time and effort to separately mount an insertion element.

On May 17, 1998, Ekins
U.S. Pat. No. 4,745,072 issued to s) discloses an immunoassay method in the field of biology, but it has a very complicated structure and is difficult to manufacture.

On December 22, 1992, in Shen Buri (Sch
No. 5,173,19 issued to Embri)
No. 3 discloses a centrifugal rotor for delivering a measured amount of liquid to a receiving chamber of the rotor.

Burtis on September 7, 1993
U.S. Pat. No. 5,242,803 issued to et al. Discloses a rotor assembly for performing analytical evaluations.

A cuvette for filling a centrifugal rotor is disclosed in US Pat. No. 5,409,665 issued to Burd on April 25, 1995.

On July 11, 1995, Ekins
US Pat. No. 5,413,009 to s) discloses a method for analyzing an analyte in a liquid.

On December 5, 1995, in Shen Buri (Schem
US Pat. No. 5,472,603 issued to
U.S. Pat. No. 5,837,048 discloses an analysis rotor with a capillary passage having an outlet duct which, by capillarity, prohibits the flow of liquid at any rotational speed and allows it above it.

However, in any of the methods, the measurement is performed only at a limited site, and the type of analysis is also limited at the same time. An example is the product of Abaxis, Inc. in the United States.

[0015]

In recent years, in medical diagnostic sites, there is a demand for a measuring device that is quick, simple, accurate, and easily available at low cost under the concept of POC (point of care). It is rare. However, in reality, mechanical and automated large-scale test solution handling systems and large-scale measuring instruments that can perform measurement by adding test solutions are mainly used in central clinical laboratories due to the ease of measurement operation. It is widely used in the medical field, and it is far from the concept of POC, and it takes a few days to know the test result.

On the other hand, in the measurement using the optical disc for analysis according to the concept of POC, it is very difficult to commercialize the product, and the number of products is very small. This is because it is easy to operate the optical disc for analysis that does not require special mechanical operation like large-scale equipment, so some enzyme reaction system assays and some immunoreaction system assays have some measurement items. Since it is limited to, the practicality is extremely low.

In Japanese Patent Application Laid-Open No. 5-5741, the parallel side surfaces of the substrate are open, and it is conceivable that the solution to be inspected may be scattered when the solution to be inspected is rotated. An example of this type of sample inspection apparatus for optically measuring a particle component is, for example, JP-A-2-26.
Japanese Patent Laid-Open No. 3-22
The analysis disc and the like shown in Japanese Patent No. 5276 are known, and the problems thereof have been solved by housing the analysis disc by a case or a cover.
There is a 01-124690 patent. However, none of the patents gave a clear answer to the sealing after the test solution was introduced into the analytical disc. In addition, it is more difficult to perform highly accurate measurement using a trace amount of blood component or the like as the test solution, which is a factor that limits the type of the test solution.

To solve these problems, USP 4, 5
There are 09 and 856 inventions. USP 4,509,85
In the sixth aspect of the invention, the optical sealing of the central substrate enables higher precision in quantification, but on the other hand, since optical signals are absorbed, there is a problem that it is not suitable for the analysis of a very small amount of the solution to be inspected. there were.

Regarding the construction method, none of the inventions is very immature, and Japanese Patent Application Laid-Open No. 5-346431 discloses a method of forming a flow path having a function equivalent to that of the channel. The test solution, which may cause the above, may be scattered. Further, in such a structure, not only the development of the measurement object becomes non-uniform, but also the reaction with the analysis reagent becomes non-uniform, and it is difficult to obtain a more precise quantitative accuracy, and it is qualitative. Alternatively, it can only have semi-quantitative performance with low quantitative accuracy, is limited to the use of those low-accuracy measurement items, and is very difficult to commercialize from the viewpoint of manufacturing cost and manufacturing tact. There was a low problem.

[0020]

In order to solve the above-mentioned problems, the optical disc for analysis of the present invention comprises a substrate for spreading a solution to be inspected, and a lid having one or a plurality of holes for introducing the solution to be inspected. An information portion integrated with both the substrate and the lid, or a part of one of the lid, and an element for guiding the solution to be inspected to the substrate through the solution introducing hole to be inspected,
One or a plurality of analysis reagents that react with the solution to be inspected introduced to the substrate through the solution to be inspected to be inspected, and an element that substantially seals the solution to be inspected introduced to the substrate;
It is characterized in that it is substantially hermetically sealed when measuring one or more light variability values with a measuring instrument.

The measuring instrument of the present invention combined with this analyzing optical disk comprises a rotating shaft capable of holding this analyzing optical disk, and a motor for supplying a rotational force to the analyzing optical disk by the rotating shaft. A light source, a light receiving element, an operating engine that operates the light source and the light receiving element, and a circuit that is substantially sufficient to operate the motor, the light source, the light receiving element, and the operating engine to perform input / output with the outside. The optical disk for analysis is provided with a case, and is measured.

[0022]

BEST MODE FOR CARRYING OUT THE INVENTION (Embodiment 1) Embodiment 1 of the present invention will be described below with reference to the drawings. In the present invention, the same effect as that of the present invention can be obtained even when the analysis reagent is not provided in the analysis optical disk and the reaction solution is obtained outside the analysis optical disk and introduced from the test solution introduction hole. The present invention is not limited to the form 1 below.

FIG. 1 is an exploded perspective view showing an optical disc for analysis according to the present invention, FIG. 2 is a perspective view showing the inside of a measuring instrument according to the present invention, and FIG. 3 is a perspective view showing an optical disc for analysis according to the present invention. FIG. 4 and FIG. 6 show the cross-sectional views of the radial direction inside the channel after the introduction of the test solution after the introduction of the solution to be inspected in the radial side cross-sectional views inside the channel according to the present invention.

More specifically, FIG. 1 is an exploded perspective view showing a hermetically-sealed optical disc for analysis according to the present invention in which an upper lid is arranged above the lid, and a positioning element is arranged at an inner peripheral portion of the optical disc for analysis. Is. Further, FIG. 2 operates a rotary shaft capable of holding an analysis optical disc, a motor for supplying a rotational force to the analysis optical disc by the rotation shaft, a light source, a light receiving element, and the light source and the light receiving element. FIG. 3 is a perspective view of a measuring instrument provided with an operating engine, the motor, the light source, the light receiving element, a circuit for operating the operating engine and inputting / outputting to / from the outside, and a housing-less perspective view. FIG. 3 shows a side cross-sectional view in a radial direction inside a channel provided with an analysis reagent before introduction of a test solution. FIG. 4 shows a side cross-sectional view in the radial direction inside the channel immediately after introduction of the test solution. FIG. 5 shows a side sectional view in the radial direction in the channel after the reaction with the test solution and the analytical reagent. FIG. 6 shows a radial side cross-sectional view within a channel under centrifugal force.

Reference numeral 1 in FIG. 1 denotes a lid which covers an upper part of a base on which a solution to be inspected is spread, and is composed of a polymer or silica-based substance. Reference numeral 2 denotes an inspection solution introducing hole in the lid. Reference numeral 3 denotes a center hole for fitting with a measuring device in the optical disc for analysis. Reference numeral 4 denotes a concave positioning element used in manufacturing. Reference numeral 5 indicates an analysis reagent provided in the analysis optical disc, and the material differs depending on the intended measurement item. Reference numeral 6 denotes an information portion which is integrated with the substrate and substantially covers information necessary for measurement. Reference numeral 7 indicates a substrate on which the solution to be inspected is spread, and is composed of a polymer or a substance whose main material is silica. Reference numeral 8 denotes a channel provided in the optical disc for analysis that develops the solution to be inspected introduced from the solution to be inspected introduction hole 2.

Reference numeral 9 in FIG. 2 is a center hole 3 for fitting in the measuring instrument.
Figure 3 shows an optical disc for analysis fitted with as a contact. 1
Reference numeral 0 denotes a rotary shaft for fitting and holding the analysis optical disk 9 through the fitting center hole 3 provided in the analysis optical disk 9. Reference numeral 11 denotes a light source necessary for reading and measuring the information portion 6, and 12 denotes a light receiving element which is a pair thereof. Reference numeral 13 denotes an operating engine that moves the light source 11 and the light receiving element 12 to arbitrary positions. Reference numeral 14 denotes a motor that rotates the rotating shaft 10. Reference numeral 15 denotes a circuit for operating the motor 14, the light source 11, the light receiving element 12, the operating engine 13, and the heat source to input and output with the outside. Reference numeral 16 denotes a heat source that substantially promotes the reaction between the analysis reagent 5 and the solution to be inspected to make it uniform. Reference numeral 17 denotes an upper lid that substantially seals the test solution introducing hole and the air hole provided in the optical disc for analysis. Reference numeral 18 denotes an air hole that facilitates the development of the solution under test in the channel 8.

Reference numeral 19 in FIG. 3 denotes the light transmittance inside the channel 8 which is represented by the width of the upward arrow. Reference numeral 20 in FIG. 4 indicates a solution to be inspected in the channel. Reference numeral 21 in FIG. 5 denotes a reaction solution in which the reaction between the analysis reagent and the test solution is substantially completed. Reference numeral 22 in FIG. 6 shows the distribution of the reaction liquid in the channel under centrifugal force.

Next, the measurement will be described with reference to the drawings. First, the analysis is started by measuring the light transmittance of the channel 8 in the absence of the solution to be inspected in the vicinity of the analysis reagent 5 with a measuring device and obtaining a blank value.

In the case of specifying the procedure by the measurer, first, the analyzing optical disk 9 is taken out from the measuring instrument, and the solution to be inspected is added to the solution introducing hole 2 to be inspected. The solution 20 to be inspected spreads in the channel 8, and after the addition, the channel 8 has an upper lid 17
Since it is hermetically sealed by the above, the reaction process can proceed without the solution to be inspected 20 flowing out from the optical disc for analysis 9. At this point, the light transmittance 19 in the channel of FIG. Analytical reagent 5 and test solution 2
The reaction of 0 is started, and the center hole 3 for attachment / detachment to and from the measuring instrument in the analysis optical disk 9 and the rotary shaft 10 are fitted and held at arbitrary positions by the concave positioning element 4.

In the measuring instrument, the analytical reagent 5 and the test solution 20 are
If necessary, the reaction is substantially heated by the heat source 16 so as to substantially accelerate and homogenize the reaction. At this point, the light transmittance 19 in the channel of FIG. 5 becomes the lowest.

When the reaction is substantially completed, the motor 14
As a result, the rotating shaft 10 rotates, and the analysis optical disk 9 also rotates. Centrifugal force affects 21 reaction solutions in 8 channels,
A slight centrifugal separation occurs, and the reaction liquid in the channel under the centrifugal force 22 is moved anywhere in the operating engine 13 by the light source 11 and the light receiving element 1.
Optically measured by 2. Quantitative results can be obtained by measuring the transmittance 19 in the channel of the reaction solution, that is, the light variability. As shown in FIG. 6, in the reaction liquid in the channel under centrifugal force 22, a relatively large substance unnecessary for the measurement is moved to the outer periphery in the eight channels and deviated from the light passage emitted from the light source 11 to obtain the most accurate determination. Can be measured.

(Second Embodiment) Next, a second embodiment of the present invention will be described with reference to FIGS. It should be noted that the present invention is not limited to the following second embodiment, and the same components as those in the above-described embodiment are designated by the same reference numerals and the description thereof will be omitted.

FIGS. 7 (a) and 7 (b) are side sectional views in the radial direction of the analyzing optical disk, and FIG. 7 (a) is a sectional view showing a state in which an outer peripheral frame is attached to the analyzing optical disk. FIG. 3B is a sectional view showing a state in which the outer peripheral frame and the water absorption frame are attached to the optical disc for analysis according to the present invention. Note in particular that both figures are cross-sectional views of the part where the channel 8 is not present.

FIG. 7A shows a substrate 7 integrated with the lid 1.
Is in a state of being sandwiched by the outer peripheral frame 23. The outer peripheral frame further seals the lid 1 and the substrate 7 which are integrated with a hard hydrophobic substance,
It has increased rigidity. Therefore, while the optical disc for analysis 9 is rotating at a high speed, the solution in the optical disc for analysis 9 can be safely measured without flowing out or being damaged.

FIG. 7 (b) shows a state in which the absorption frame 24 is added to the state of FIG. 7 (a). Here, the absorption frame 24 is first installed and the outer peripheral frame 23 is attached to the lid 1 and the substrate 7 which are integrated with each other, the sealing property and the rigidity are the same as those of FIG. 7A, and the optical disc for analysis is used. When the solution in the optical disc 9 for analysis flows out while the 9 is rotating at a high rotation speed, the outflowing solution is absorbed by the absorption frame and can be measured safely.

(Third Embodiment) Next, a third embodiment of the present invention will be described with reference to FIGS. The present invention is not limited to the following third embodiment, and the same components as those of the above-described embodiment are designated by the same reference numerals and the description thereof will be omitted. 8 (a)-(d)
FIG. 8 is a side sectional view in the radial direction of the optical disc for analysis, which is almost the same as the optical disc for analysis shown in FIGS.
FIG. 3A is a side cross-sectional view in a radial direction inside a channel including an inner lid, a temporary fixing substance, a protrusion for fixing the inner lid at an arbitrary position, and an analysis reagent before introduction of a test solution. FIG. 8B shows a side cross-sectional view in the radial direction in the channel immediately after introduction of the solution to be inspected.
FIG. 8C shows a side cross-sectional view in the radial direction in the channel after the reaction with the test solution and the analytical reagent. FIG. 8 (d) shows a side sectional view in the radial direction inside the channel under centrifugal force.

Reference numeral 25 in FIG. 8A indicates an inner lid, which moves to the outer peripheral side of the channel 8 under centrifugal force. 26 is the inner lid 21
Shows a temporary fixing substance for temporarily fixing, and is easily melted in a solution. Reference numeral 27 denotes a protrusion that holds the inner lid at an arbitrary position.

Next, those effects will be described with reference to the drawings. First, the solution to be inspected is added to the solution introducing hole 2 to be inspected, and the solution to be inspected 20 is spread in the channel 8. After the addition, the channel 8 is sealed by the upper lid 17, so that the reaction process can proceed without the solution 20 to be inspected flowing out from the optical disc 9 for analysis. When the reaction between the analysis reagent 5 and the solution to be inspected 20 is started, the temporary fixing substance 26 is melted, and the inner lid 25 moves freely. When the reaction is substantially completed, the rotating shaft 10 is rotated by the motor 14 as described in the first embodiment, and the optical disc for analysis 9
Also rotates. Centrifugal force acts on the reaction solution 21 in the channel 8 to cause a light centrifugal separation, and the inner lid 25 also moves to the channel 8
Moves to the outer peripheral side. The inner lid 25 stops moving even under centrifugal force by the protrusion 27, becomes the inner lid of the solution to be inspected 2 to be inspected, and enhances the hermeticity of the channel 8 to further enhance safety.

(Fourth Embodiment) Next, a fourth embodiment of the present invention will be described with reference to FIG. It should be noted that the present invention is not limited to the following Embodiment 4, and the same reference numerals are used for the same configurations as those of the above-described embodiment, and description thereof will be omitted.

FIG. 9 shows a side sectional view of a press for producing the substrate 7 and the lid 1 of the optical disc for analysis 9 according to the present invention. FIG. 9 is a side sectional view of the substrate 7 or the lid 1 immediately before production. 28 in FIG. 9 is a force frame (force).
frame) and does not deform even under stress or temperature change inside the press. 29 indicates a heating / cooling mechanism, which heats quickly,
Proceed with the cooling cycle. Reference numeral 30 denotes an embossing mold. 31 shows a material. Reference numeral 32 denotes an operating mechanism of the press machine.

Next, those steps will be described with reference to the drawings. First, the heating / cooling mechanism 29 heats and heat is transferred to the mold 30. When the material 31 is placed and heated to an appropriate temperature, the operating mechanism 32 moves the mold 30 together with the heating / cooling mechanism 29, the mold 30 comes into contact with the material 31, and the material 31 deforms in the shape of the mold 30. After a certain time, the mold 30 starts cooling while being in contact with the material 31, and after a certain time, the material 31 is taken out from the press machine. Even after being taken out, the material 31 is deformed to some extent and has an arbitrary shape. The material 31 is preferably a polymer material or a material containing silica as a main component in consideration of cost and manufacturing simplicity, and can be processed with high precision.

(Fifth Embodiment) Next, a fifth embodiment of the present invention will be described with reference to FIGS. 10 (a) and 10 (b). The present invention is not limited to the following fifth embodiment, and the same components as those in the above-described embodiment are designated by the same reference numerals and the description thereof will be omitted. Figure 10 (a)
~ (B) shows a conceptual diagram of obtaining the latest quality control information from the outside of the measuring instrument regarding the measurement operation including the correction at the time of analysis,
FIG. 7A is a conceptual diagram for obtaining the latest quality control information by wire communication according to the present invention, and FIG. 9B is a conceptual diagram for obtaining the latest quality control information by wireless communication according to the present invention.

FIG. 10A shows a state in which the server 33 having the latest quality control information and the measuring instrument 34 are connected via wired communication. FIG. 10B shows a state in which the server 33 having the latest quality control information and the measuring instrument 34 are connected via wireless communication. 10A and 10B, since the server 33 having the latest quality control information and the measuring device 34 are connected, no troublesome correction is required by the measurer, and the operator side does not It is very convenient to grasp the needs. Furthermore, the most accurate quantitative measurement is possible because it can be measured based on the latest quality control information.

[0044]

The following examples further illustrate in detail the method of practicing the present invention. The present invention is not limited to the examples below. (Preparation of Optical Disc for Analysis of Urinary hCG Measurement) Antibody-antigen analysis optics containing an anti-hCG-β antibody-immobilized site on a plastic substrate and an analysis reagent for a complex of anti-hCG-α antibody and colloidal gold A disc was manufactured.

Example 1. The optical disk fabrication mold (stamper) is placed in a mold, liquid polycarbonate is injected into the sealed mold, the mold is cooled, and then the robot arm metalizes the transparent disk (either substrate or lid). Transferred to the process to coat the metal layer. Thus, the transparent disc was completed.

Example 2. Preparation of analytical reagent Anti-hCG-diluted with phosphate buffer solution to adjust its concentration
A β antibody solution was prepared. This antibody solution was applied to a part of the channel of the transparent disk using a solution discharge device.
As a result, an antibody-immobilized site for detection was obtained on the substrate. After drying this substrate, T containing 1% skim milk
The ris-HCl buffer solution was applied onto the transparent disk with a solution discharge device and gently shaken in a high humidity environment for 30 minutes. After 30 minutes, the substrate was transferred to a Tris-HCl buffer solution tank and shaken gently for 10 minutes, and then another Tris-H solution was added.
The substrate was washed by gently shaking for 10 minutes in a Cl buffer solution bath. After washing twice, the substrate was taken out of the washing liquid and dried at room temperature.

Gold colloids were prepared by adding 1% citric acid solution to a 100 ° C. solution of 0.01% chloroauric acid in reflux. Reflux was continued for 30 minutes before cooling.
Anti-hCG-α antibody was added to the gold colloidal solution adjusted to pH 9 with 0.2 M potassium carbonate solution, and the mixture was stirred for several minutes, and then 10% BSA (bovine serum albumin) was added.
An antibody-gold colloidal complex (labeled antibody) was prepared by adding solution pH 9 in an amount of 1% final and stirring. The labeled antibody solution was centrifuged at 4 ° C. and 20000 G for 50 minutes to isolate the labeled antibody, which was suspended in a washing buffer (1% BSA / phosphate buffer) and then centrifuged. The labeled antibody was washed and isolated.
The labeled antibody was suspended in a washing buffer and filtered through a 0.8 μm filter to obtain an initial gold colloid solution amount of 1
It was prepared 1/0 and stored at 4 ° C.

The labeled antibody solution was set in a solution discharge device and applied to a position apart from the antibody-immobilized position on the anti-hCG-β antibody-immobilized dry substrate, and then the substrate was dried. As a result, a labeled antibody holding site was obtained on the immobilized substrate. Thus, the substrate of the optical disc for antibody-antigen analysis was completed.

Example 3. Preparation of Optical Disk for Antibody-Antigen Analysis A 0.5 mm-thick lid was laminated on a substrate of an optical disk for antibody-antigen analysis having a thickness of 0.5 mm and a double-sided adhesive sheet having the same shape as the disk was laminated and attached. . Then, the laminated disks were integrated using a pressure roller and tightly sealed to manufacture. Thus, an optical disc for antibody-antigen analysis was produced.

Example 2. Sample Preparation By adding a known concentration of hCG solution to human urine,
Various known concentrations of hCG solution were prepared.

Example 3. H in the test solution introducing hole of the optical disc for measuring antibody-antigen analysis
5 μl of CG-containing urine was added to the optical disc for antibody-antigen analysis and developed in the outer peripheral direction of the channel to cause an antigen-antibody reaction to cause a color reaction in the antibody-immobilized portion. External type CD-R / RW drive (LK-RW) with a partial change in color development after 5 minutes from the addition of the test solution to this test piece
7585UZ; made by Matsushita Judo Electronics Co., Ltd.)
The light transmittance was calculated.

100, 1000, 10000 U / l h
Urine containing CG was added to the test solution introduction hole and developed. The light transmittance of the antibody-immobilized portion in the channel for urine of each hCG concentration was measured by an external CD-R / RW drive with some changes. The light transmittance at a wavelength of 780 ± 10 nm was measured, and the light transmittance was substituted into a previously prepared calibration curve showing the relationship between the hCG concentration and the light transmittance. The result is shown in FIG. In FIG. 11, the liquid sample is added to the antibody-antigen analysis optical disk, and the concentration of the analyte is converted based on the measured value of the light transmittance after 5 minutes. From this result, it was confirmed that the measurement of the optical disc for antibody-antigen analysis had high quantification accuracy.

[0053]

As described above, according to the optical disc for analysis of claim 1 of the present invention, the lid having the substrate for spreading the solution to be inspected and having one or a plurality of holes for introducing the solution to be inspected. An information portion integrated with the substrate and / or the lid, or a part thereof, an element for guiding the inspection solution to the substrate through the inspection solution introducing hole, and the inspection solution introducing hole One or more analysis reagents that react with the test solution guided to the substrate, an element that substantially seals the test solution that is guided to the substrate, and one or more light changes by the measuring instrument The optical disc for analysis is characterized in that it is substantially sealed at the time of measuring the sex.

Since the lid has an inspection solution introducing hole and is substantially hermetically sealed, and the simple structure allows the manufacturing process of the existing optical disk to be utilized, the manufacturing cost can be reduced by reducing the manufacturing man-hours as compared with the conventional analyzers. The effect that can be obtained is obtained. With these, it is possible to provide an optical disc for analysis with higher accuracy and lower cost.

Further, according to the optical disc for analysis of the second aspect, at least one of biological, chemical, molecular biological, biochemical or a combination thereof is provided between the lid and the substrate. The optical disc for analysis according to claim 1, comprising the analysis reagent, wherein various measurement items can be analyzed by using various kinds of analysis reagents, and By using multiple analysis reagents to enable analysis of multiple test solutions,
It is possible to provide an optical disc for analysis with higher utilization.

According to the third aspect of the invention, one or more channels connected to the test solution introducing hole are provided between the lid and the substrate, and the test solution is guided to the analytical reagent. The optical disc for analysis according to claim 1, wherein the inspection solution introducing hole and one or more channels are provided, so that a required portion of the inspection solution can be operated at a higher speed. Moreover, by guiding the analysis reagent uniformly, it is possible to provide a more accurate analysis optical disc.

According to a fourth aspect of the present invention, the function of guiding the test solution from the test solution introducing hole to the substrate is connected to the one or more chambers through the test solution introducing hole, The optical disc for analysis according to claim 1, wherein a solution to be inspected is led to the analysis reagent, and various measurement items are obtained by using a plurality of kinds of analysis reagents in each of the plurality of chambers. It is possible to analyze a plurality of solutions to be inspected by using a plurality of analysis reagents of a limited type, and thus it is possible to provide an optical disk for analysis with higher utilization.

According to a fifth aspect of the present invention, one or more outer peripheries capable of integrating and sealing the outer circumference of the optical disc for analysis with a hydrophobic substance substantially harder than the substrate and the lid. The optical disc for analysis according to claim 1, further comprising a frame, which substantially enhances rigidity and hermeticity of the optical disc for analysis, wherein the optical disc for analysis is integrated with a hard hydrophobic substance. By providing one or more outer peripheral frames that can be hermetically sealed, it is possible to provide an optical disc for analysis that does not break, and thus does not cause the solution to be inspected to flow out.

According to a sixth aspect of the present invention, one or more water absorption frames made of a hydrophilic substance are provided between the outer peripheral frame and the analysis optical disc, and the analysis optical disc is used to detect the inspection target. The analysis optical disk according to claim 5, wherein the water absorption frame prevents the solution to be inspected from flowing out when the solution flows out. By preventing the outflow of the solution to be inspected at the water absorption frame at the time of outflow of the solution to be inspected from the analysis optical disc damaged by providing a water absorption frame in between, safer, sealed, simple and It is possible to provide a low-cost analysis optical disc.

In the invention according to claim 7, one or a plurality of inner lids are provided inside the function of guiding the solution to be inspected to the substrate through the solution introducing hole to be inspected, and the inner lid is for analysis. When the optical disc is rotated, it can move in the channel and substantially closes the test solution introducing hole,
The optical disc for analysis according to claim 3, which further enhances hermeticity, wherein one or more inner lids are provided inside the function of guiding the solution to be inspected to the substrate, It is possible to provide a simple and low-cost optical disc for analysis which has substantially improved hermeticity and requires no operation.

Further, according to the present invention, the function of substantially dissolving the optical disc for analysis in the solution to be inspected at the start of rotation of the optical disc to be inspected is provided from the introduction hole of the solution to be inspected to the optical disc for analysis. The optical disc for analysis according to claim 7, characterized in that the inner lid is provided on the inner peripheral side around the inner lid in the channel, and the inner lid is temporarily fixed until the solution to be inspected is introduced. Yes, the inner lid is temporarily fixed until the test solution is introduced, and has a function of being substantially dissolved by the test solution at the start of rotation of the optical disc for analysis, which enables simple sealing. It is possible to provide a method for producing an optical disc for cost analysis.

Further, according to the invention of claim 9, after the introduction of the solution to be inspected, the temporarily fixed inner lid is moved to the outer peripheral side by centrifugal force when the optical disc for analysis is rotated,
8. One or more protrusions for locking the inner lid at any position within the channel.
The optical disc for analysis according to claim 8, wherein the inner lid is provided with a projection that can be stopped at an arbitrary position after being moved to the outer peripheral side by the centrifugal force of, thereby ensuring the hermeticity. Therefore, a safer optical disc for analysis can be provided.

According to the tenth aspect of the present invention, the solution to be inspected has a characteristic of substantially an air hole that allows the gas in the channel to be freely discharged when the solution to be inspected is introduced. An upper lid that can be placed on the solution introducing hole and that substantially seals the channel has a function of integrating with the analysis optical disc, and the analysis optical disc does not substantially interfere with the measurement. The optical disc for analysis according to claim 3, wherein the inspection solution introducing hole has a characteristic of substantially an air hole capable of freely discharging a gas in the channel, and an upper lid for sealing the channel. It is possible to provide a safer optical disc for analysis, since it is possible to enhance the hermeticity without hindering the movement of the liquid in the channel and to surely improve the sealing property.

According to the invention of claim 11, a part of the inside of the channel and / or the chamber is made of a material having a substantially higher reflectance than the material of the lid and the material of the substrate. 5. The method according to claim 3, wherein
The optical disc for analysis described above, and since the inside of the channel and / or the chamber is made of a material having a high reflectance, an optical disc for analysis with higher sensitivity and higher accuracy can be provided.

According to a twelfth aspect of the present invention, there is provided the analysis optical disk according to the first aspect, which is provided with a positioning element used at the time of manufacturing and / or at the time of measurement. Therefore, in the measurement of the optical disc for analysis, by providing the positioning element, it is possible to provide the optical disc for analysis that can be manufactured and handled more easily.

According to the thirteenth aspect of the present invention, the low-molecular-weight reaction system assay containing ions is carried out by freely or at a limited site on the substrate, the test solution and the analytical reagent. The optical disc for analysis according to claim 1, characterized in that the light variability is measured after completion of the reaction, and a small molecule reaction system assay containing ions is simply and substantially reacted. After the completion, the optical disc for analysis can be provided with higher accuracy by measuring the light variability.

According to the fourteenth aspect of the present invention, the enzyme reaction system assay is carried out freely or at a limited site on the substrate with the test solution and the analytical reagent,
The optical disc for analysis according to claim 1, characterized in that the light variability is measured after the reaction is substantially completed, and the enzymatic reaction system assay is simply completed after the reaction is completed. By measuring the light variability, a more accurate optical disc for analysis can be provided.

According to a fifteenth aspect of the present invention, the antibody-antigen reaction system assay is carried out by freely or at a limited site on the substrate the test solution and the analytical reagent, The optical disc for analysis according to claim 1, wherein the light variability is measured after completion of the reaction.
It is possible to provide a more accurate optical disc for analysis by simply measuring the light variability after substantially completing the reaction of the antibody-antigen reaction system assay.

According to the sixteenth aspect of the present invention, the base sequence-antigen reaction system assay is carried out substantially freely or at a limited site on the substrate with the test solution and the analytical reagent. The optical disc for analysis according to claim 1, characterized in that the light variability is measured after the reaction is completed, and the base sequence-antigen reaction system assay is simply and substantially after completion of the reaction. By measuring the light variability, a more accurate optical disc for analysis can be provided.

According to a seventeenth aspect of the present invention, the analytical optical disc according to the sixteenth aspect is characterized in that the analytical reagent of the base sequence-antigen reaction system assay contains an aptamer. Since an aptamer is contained in the analysis reagent of the base sequence-antigen reaction system assay, a more accurate optical disc for analysis can be provided.

According to the eighteenth aspect of the invention, in the base sequence-base sequence reaction system assay, the test solution and the analysis reagent are freely or limited on the substrate and complemented. The optical disc for analysis according to claim 1, wherein the photovariability is measured after the reaction with the binding reagent that binds only to the sex-bonded site is substantially completed. By simply and substantially completing the reaction of the sequence-base sequence reaction system assay and then measuring the light variability, a more accurate optical disc for analysis can be provided.

According to a nineteenth aspect of the invention, there is provided the optical disc for analysis according to the eighteenth aspect, wherein the binding reagent is an intercalator, and the binding reagent is an intercalator. Since it is a calendar, a highly accurate optical disc for analysis can be provided.

According to a twentieth aspect of the present invention, the binding reagent is a labeled antibody,
The optical disc for analysis according to claim 18, wherein the binding reagent is a labeled antibody, whereby a more precise optical disc for analysis can be provided.

According to the twenty-first aspect of the invention, in the cell-antibody reaction system assay, the test solution and the analysis reagent are freely reacted on the substrate or at a limited site, and substantially react. The optical disc for analysis according to claim 1, characterized in that the light variability is measured after the completion of the cell-antibody reaction system assay after the reaction is substantially completed. By measuring the variability, a highly accurate optical disc for analysis can be provided.

According to the twenty-second aspect of the invention, the prokaryote, the eukaryote, or the eukaryotic cell is cultured between the lid and the substrate, and after the culture, biological, chemical, One or more molecular biological or biochemical reagents or one or more mixed reagents in which any one of them is combined is added from the test solution introducing hole, and after the reaction is substantially completed, the mixture is centrifuged to obtain a culture. The optical disc for analysis according to claim 1, characterized in that the substantial life or death of the is detected by the measurement of the photovariability, and the assay of cultured cells can be carried out in the optical disc for analysis. Can provide an optical disc for analysis with a wider range of functions.

Further, according to the invention of claim 23, before setting with the measuring instrument, all the holes of the channel filled with the solution are closed by an upper lid. The optical disc for analysis of
An optical disc for analysis having a safer function can be provided by substantially completely sealing the hole with an upper lid before measurement.

According to a twenty-fourth aspect of the invention, the optical disc for analysis comprises the substrate and the lid, and both or one of them is mainly composed of a high heat-soluble polymer. The optical disc for analysis according to claim 1, wherein a high-melting polymer is used as a main material to provide a lower-cost optical disc for analysis.

According to a twenty-fifth aspect of the present invention, the optical disc for analysis comprises a substrate and a lid, and either or both of them are produced by a press machine. The optical disc for analysis described in (1) above, and by manufacturing the main components of the optical disc for analysis by a press machine, a lower cost optical disc for analysis can be provided.

According to the invention of claim 26, the polymer is biodegradable.
4 is an optical disc for analysis, and the main component of the optical disc for analysis is made of biodegradable polymer, so that an optical disc for analysis with lower environmental cost can be provided.

According to a twenty-seventh aspect of the present invention, the analysis optical disk according to the first aspect is characterized in that the substrate and / or the lid is made of silica as a main material. Since the main material of the optical disc for analysis is made of silica, a more accurate optical disc for analysis can be provided.

According to a twenty-eighth aspect of the present invention, one or more positioning elements are provided on the inner circumference or the outer circumference or both of the analysis optical discs so that the positioning can be facilitated during manufacturing. According to another aspect of the present invention, there is provided the optical disc for analysis according to claim 1, and by providing a positioning element on the optical disc for analysis, an optical disc for analysis with higher accuracy can be provided.

According to a twenty-ninth aspect of the invention, the positioning element is a concave type or a convex type or a combination thereof, and is processed at the time of manufacturing the lid and the substrate. The optical disc for analysis described in (1) above, and by providing a concave or convex positioning element or a combination of positioning elements on the optical disc for analysis, a more accurate optical disc for analysis can be provided.

According to the thirtieth aspect of the invention, a plurality of the channels are connected to the chambers having different sizes in the optical disc for analysis, and the same kind of the analysis is performed in the chambers having different sizes. Since the volumes of the chambers, which are provided with the same amount of reagents and have different sizes when the test solution is introduced, are different, the analytical reagent is diluted with the test solution at different magnifications, and the reaction is substantially completed. 5. An analytical optical disk according to claim 3, wherein a calibration curve is simply obtained at the time of analysis and measurement. The analytical curve is easily prepared after the reaction in the analytical optical disk. It is possible to provide an optical disc for analysis that can be quantitatively measured with high accuracy if required.

According to a thirty-first aspect of the present invention, the same type of the analytical reagent is provided in a branched channel extending in a branched structure having different lengths from the test solution introducing hole in the disc. The same amount is provided for each, and since the lengths of the branched channels are different when the test solution is introduced, the analytical reagent is diluted with the test solution at different magnifications, and after the reaction is substantially completed. The analytical optical disc according to claim 3, characterized in that a calibration curve is easily obtained at the time of analysis measurement, and the calibration curve is easily obtained after the reaction is completed in the analysis optical disc. It is possible to provide an optical disc for analysis capable of highly accurate quantitative measurement.

According to the thirty-second aspect of the present invention, the information portion is a digital signal, and a content that substantially covers information necessary for analysis is recorded by an optical method, a magnetic method, or both methods. The optical disc for analysis according to claim 1, wherein the optical disc for analysis is a digital signal optically or magnetically,
Alternatively, by recording the contents that substantially cover the information required for the analysis by both methods, it is possible to provide a simpler and more accurate quantitative measurement optical disc for analysis.

A measuring machine according to a thirty-third aspect of the invention is a rotating shaft capable of holding the optical disc for analysis according to the first aspect, a motor for supplying a rotational force to the optical disc for analysis by the rotational shaft, and a light source. A light receiving element, an operating engine for operating the light source and the light receiving element, a circuit substantially sufficient for operating the motor, the light source, the light receiving element, and the operating engine to perform input / output with the outside. It is equipped with a body and measures the optical disc for analysis in the previous period, and the necessary parts are almost the same as the optical disc reading device generally used for computers, and it is a lower cost and simpler analysis optical disc. A system using a disc can be provided.

According to a thirty-fourth aspect of the present invention, the light transmissivity, the light reflectivity, the photothermal conversion property or a combination thereof is used to measure the light variability. It is a measuring instrument, and it is possible to provide a simpler low-cost and highly-accurate measuring instrument by measuring the light transmissivity, the light reflectivity, the light variability consisting of the photothermal conversion property or a combination thereof. .

The thirty-fifth aspect of the present invention is characterized by comprising photoacoustic spectroscopy, thermal lens spectroscopy, photothermal deflection spectroscopy, photothermal reflection spectroscopy, photothermal displacement spectroscopy, photothermal radiation spectroscopy, or a combination thereof. 34. The measuring device according to claim 33, which is non-contact with the measuring method and has higher sensitivity, such as photoacoustic spectroscopy, thermal lens spectroscopy, photothermal deflection spectroscopy, photothermal reflection spectroscopy, photothermal displacement spectroscopy, photothermal radiation spectroscopy, or By using the measurement technique of the combination, a highly accurate measuring device can be provided.

According to a thirty-sixth aspect of the invention, there is provided a non-contact heat source for substantially homogenizing and promoting the reaction of the analytical reagent provided on the optical disc for analysis. 34. The measuring device according to claim 33, which comprises a non-contact type heat source for substantially homogenizing and promoting the reaction in the optical disc for analysis, and is capable of performing quantitative measurement with high accuracy. Can be provided.

According to a thirty-seventh aspect of the present invention, the non-contact type heat source comprises one or a combination of a laser, a carbon lamp, an IH heater, a heating wire, and a microwave. 37. The measuring device according to claim 36, wherein one or a combination of a laser, a carbon lamp, an IH heater, a heating wire and a microwave for substantially homogenizing and promoting the optical disc for analysis is used. By providing the non-contact heat source consisting of, it is possible to provide a measuring device capable of performing quantitative measurement with high accuracy.

According to a thirty-eighth aspect of the invention, there is provided a vibration source for substantially homogenizing and promoting the reaction of the analytical reagent provided on the optical disc for analysis. The measuring device according to claim 33 is provided, and by providing a vibration source for substantially homogenizing and promoting the optical disc for analysis, it is possible to provide a measuring device capable of quantitative measurement with high accuracy.

According to a thirty-ninth aspect of the invention, there is provided the measuring instrument according to the thirty-third aspect, characterized in that the measuring instrument is provided with an ultraviolet lamp for preventing infection of a person to be measured during non-operation. Therefore, a safer measuring instrument can be provided by being equipped with an ultraviolet lamp for preventing infection of the measurer.

The invention according to claim 40 is characterized in that it is provided with a photocatalyst coating layer for exhibiting a permanent sterilizing / sterilizing action and preventing the infection of the measurer. The measuring device described above is provided, and a safer measuring device can be provided by providing the photocatalyst coating layer for preventing infection of the measurer.

Further, according to the invention of claim 41, it is partly manufactured by an antibacterial material for exhibiting a permanent sterilizing / sterilizing action and preventing infection to a measuring person.
The measuring device according to claim 33 is provided, and a safer measuring device can be provided by being partly manufactured with an antibacterial material for preventing infection of the measurer.

According to a forty-second aspect of the present invention, while the optical disc for analysis rotates about the center as an axis, one or more light beams scan the optical disc for analysis in a radial direction,
34. A measuring device according to claim 33, characterized in that the lowest measuring operation resulting from measuring the light variability derived therefrom is advanced on the basis of additional information obtained in advance from outside the measuring device. Therefore, it is possible to provide a measuring instrument that can be quantitatively measured with high accuracy and with a simple operation by a measurement operation that is advanced based on additional information obtained from the outside of the measuring instrument in advance.

According to a thirty-third aspect of the present invention, one or more light beams scan the analysis optical disc in the radial direction while the analysis optical disc is rotating around the center.
34. The measurement operation, which consists in measuring the light variability derived therefrom, is advanced on the basis of a digital signal obtained in advance from the information part of the analysis optical disc. It is possible to provide a measuring instrument which is easy to operate and can perform high-precision quantitative measurement by a measuring operation which is advanced based on a digital signal obtained from the information portion of the optical disc for analysis in advance. .

According to the 44th aspect of the present invention, while the optical disc for analysis is rotated about the center, one or more light beams scan the optical disc for analysis in the radial direction,
34. The minimum measurement operation of measuring the light variability derived therefrom is advanced based on information obtained from the outside through communication such as the Internet or a mobile phone in advance. It is a measuring instrument, and it is possible to provide a measuring instrument that can be quantitatively measured with high accuracy by simple operation by a measurement operation that is advanced based on information obtained from the outside in advance through communication such as the Internet.

According to a forty-fifth aspect of the invention, one or more light beams scan the analysis optical disc in a radial direction while the analysis optical disc is rotating around the center.
34. The measuring device according to claim 33, characterized in that the minimum measurement operation resulting from measuring the light variability derived therefrom is advanced based on information obtained from the outside through a magnetic card in advance. Therefore, it is possible to provide a measuring device that can be quantitatively measured with high accuracy by a simple operation by a measurement operation that is advanced based on information obtained from the outside through a magnetic card in advance.

According to the forty-sixth aspect of the present invention, when the information of the measurement operation obtained from the outside in advance cannot be input, a manual input instruction is externally output to the measurer, and the manual input is performed. 34. The measuring device according to claim 33, characterized in that the measuring operation is carried out. The measuring device is informed of a manual input instruction when the external acquisition is impossible in advance, and the manual input information is also included. It is possible to provide a measuring instrument that can be quantitatively measured with high accuracy and easy operation by the measurement operation that is advanced.

According to the forty-seventh aspect of the invention, in the scanning measurement of the optical disc for analysis, the light change in the vicinity of the analysis reagent at a distance that does not substantially affect the light variability. 34. The measuring device according to claim 33, wherein a blank value is reflected in an indexing measurement result by measuring the property with the light source used for the actual scanning measurement. It is possible to provide a measuring instrument which is easy to operate and can perform quantitative measurement with high accuracy by the measurement operation which is performed based on the index of the blank value in the vicinity of the analytical reagent and reflecting it in the measurement result.

According to the invention as set forth in claim 48, based on the latest quality control information obtained from a sales company, a manufacturing company or both through communication such as the Internet or a mobile phone from the outside before and after the analysis operation. 34. The measuring instrument according to claim 33, characterized in that the measuring operation including the correction is carried out, the selling company or the manufacturing company before or after the analyzing operation through communication such as the Internet or a mobile phone from the outside. Alternatively, by advancing the measurement operation including the correction on the basis of the latest quality control information obtained from both of them, it is possible to provide a measuring instrument which is simple in operation and capable of quantitative measurement with high accuracy.

According to the 49th aspect of the invention, when the solution to be inspected is blood, the centrifugal force generated by rotating the optical disc for analysis at the time of measurement causes the centrifugal force generated in the optical disc for analysis to be As a result of the test solution, the blood cell component from the outer peripheral side of the channel, the hematocrit value can be measured substantially simultaneously with the original analysis item, and the result is added to the calculation process as one of the correction items and highly accurate quantification 34. Measuring device according to claim 33, characterized in that it contributes to the measurement.
Therefore, by adding the hematocrit value to the analysis optical disc as one of the correction items in the calculation process, it is possible to provide an analysis optical disc capable of more accurate quantitative measurement.

According to the 50th aspect of the invention, there is provided a function of recording items such as measurement values, analysis results, IDs, etc. desired by the measurer in the information portion of the optical disc for analysis by a measuring instrument. According to another aspect of the present invention, there is provided an optical disc for analysis according to claim 1, wherein an arbitrary information is recorded on the optical disc for analysis to provide an optical disc for analysis more suitable for an information society.

[Brief description of drawings]

FIG. 1 is an exploded perspective view showing a hermetically-sealed optical disc for analysis according to the present invention in which an upper lid is arranged on an upper portion of the lid and a positioning element is arranged on an inner peripheral portion of the optical disc for analysis.

FIG. 2 illustrates a rotating shaft capable of holding an analyzing optical disc, a motor for supplying a rotational force to the analyzing optical disc by the rotating shaft, a light source, a light receiving element, and the light source and the light receiving element. A perspective view without a housing showing an operating engine, a circuit for operating the motor, the light source, the light receiving element, the operating engine, and a circuit for inputting / outputting to / from the outside, and a measuring instrument provided with a heat source.

FIG. 3 is a side cross-sectional view before introduction of the test solution immediately after introduction of the test solution in the radial direction in the channel provided with the analysis reagent.

FIG. 4 is a side cross-sectional view in the radial direction in the channel.

FIG. 5 is a side cross-sectional view after reaction of the test solution in the radial direction of the channel with the analytical reagent.

FIG. 6 is a side sectional view in the radial direction of the channel under centrifugal force.

FIG. 7 (a) is a sectional view showing a state where an outer peripheral frame is attached to an analysis optical disc, and (b) is a sectional view showing a state where an outer periphery frame and a water absorption frame are attached to the analysis optical disc of the present invention.

FIG. 8 (a) is a side cross-sectional view before introduction of a solution to be tested in a radial direction in a channel, which is provided with an inner lid, a temporary fixing substance, a protrusion for fixing the inner lid at an arbitrary position, and an analysis reagent. (C) Side cross-sectional view immediately after introduction of the solution to be inspected (c) Side cross-sectional view after reaction of the test solution with analytical reagent in the radial direction inside the channel (d) Side cross-sectional view in radial direction inside the channel under centrifugal force

FIG. 9 is a substrate 7 of the optical disc for analysis 9 according to the present invention.
Side sectional view of a press for making the lid 1

FIG. 10 (a) is a conceptual diagram for obtaining additional information by wired communication according to the present invention, and (b) is a conceptual diagram for obtaining additional information by wireless communication according to the present invention.

FIG. 11 is a relationship diagram between hCG concentration and light transmittance.

[Explanation of symbols]

1 lid 2 Inspected solution inlet 3 center hole 4 Positioning element 5 Analytical reagents 6 information part 7 substrate 8 channels 9 Optical disc for analysis 10 rotation axis 11 light source 12 Light receiving element 13 working institutions 14 motor 15 circuits 16 heat source 17 Top cover 18 air holes 19 Transmittance 20 Inspected solution 21 Reaction liquid 22 Distribution of reaction liquid in the channel under centrifugal force 23 Perimeter frame 24 Water absorption frame 25 Inner lid 26 Temporary fixing substance 27 protrusions 28 Force Frame 29 Heating / cooling mechanism Type 30 31 Material 32 operating mechanism 33 servers 34 Measuring instrument

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 33/543 595 G01N 33/543 595 F term (reference) 2G054 AA06 AB03 AB04 CA21 CA22 GB03 2G059 AA05 BB12 CC16 DD04 DD05 DD13 DD16 EE01 GG10 KK01 MM09 MM12 MM15 4B029 AA07 AA23 BB01 BB16 BB20 CC01 FA11 FA12 FA13 FA15

Claims (50)

[Claims] 1. A lid having a substrate for spreading a solution to be inspected and having one or a plurality of holes for introducing the solution to be inspected, and the substrate and / or the lid, or a part of either one of them. An information part, an element that guides the test solution to the substrate through the test solution introducing hole, and one or more analysis reagents that react with the test solution that is guided to the substrate through the test solution introducing hole And an element for substantially sealing the solution to be inspected introduced to the substrate, and an optical disk for analysis, which is substantially sealed when measuring one or a plurality of light changeability by a measuring instrument. 2. Biological, chemical, between the lid and the substrate,
The optical disc for analysis according to claim 1, further comprising one or more of the analysis reagents of molecular biology, biochemistry or a combination thereof. 3. The method according to claim 1, further comprising one or more channels connected to the test solution introducing hole between the lid and the substrate, for guiding the test solution to the analysis reagent. Optical disc for analysis. 4. The function of guiding the test solution to the substrate through the test solution introducing hole is connected to one or more chambers through the test solution introducing hole to guide the test solution to the analytical reagent. The optical disc for analysis according to claim 1, wherein: 5. An optical disc for analysis, comprising one or more outer peripheral frames capable of integrating and sealing the outer periphery of the optical disc for analysis with a hydrophobic substance substantially harder than the substrate or the lid. The optical disc for analysis according to claim 1, wherein the rigidity and the hermeticity of the optical disc are substantially increased. 6. A water-absorbing frame made of a hydrophilic substance is provided between the outer peripheral frame and the analysis optical disk, and when the solution to be inspected flows out from the analysis optical disk, the water-absorbing frame is used for the analysis. The optical disc for analysis according to claim 5, wherein the outflow of the solution to be inspected is prevented. 7. The inside of the function of guiding the solution to be inspected to the substrate through the solution introducing hole to be inspected is provided with one or a plurality of inner lids, and the inner lid is provided with the channel when the optical disc for analysis is rotated. 4. The optical disc for analysis according to claim 3, wherein the optical disc for analysis is capable of moving inside and substantially closes the introduction hole for the solution to be inspected to further enhance the hermeticity. 8. The function of substantially dissolving in the solution to be inspected at the start of rotation of the optical disc for analysis is provided on the inner peripheral side of the optical disc for analysis from the introduction hole of the solution to be inspected,
The optical disc for analysis according to claim 7, wherein the optical disc for analysis is provided around the inner lid in the channel, and the inner lid is temporarily fixed until the solution to be inspected is introduced. 9. After the introduction of the solution to be inspected, the temporarily fixed inner lid is moved to the outer peripheral side by a centrifugal force when the optical disc for analysis is rotated, and the inner lid is moved to an arbitrary position in the channel. 9. An optical disc for analysis according to claim 7 and claim 8, characterized in that it is provided with one or more projections which can be stopped by means of. 10. The test solution introducing hole has substantially the characteristics of an air hole that allows the gas in the channel to be freely discharged when the test solution is introduced, and can be installed on the test solution introducing hole. 4. The analytical use according to claim 3, wherein the lid for sealing the channel has a function of being integrated with the analytical optical disc, and the analytical optical disc does not substantially interfere with the measurement. Optical disc. 11. The method according to claim 3, wherein a part of the inside of the channel and / or the chamber is made of a material having substantially higher reflectance than the material of the lid and the material of the substrate. The optical disc for analysis described. 12. The optical disc for analysis according to claim 1, further comprising a positioning element used at the time of manufacturing and / or at the time of measuring. 13. A low-molecular-weight reaction system assay containing ions is carried out at a limited site where the solution to be inspected and the analytical reagent are freely or limited on the substrate, and after the reaction is substantially completed,
The optical disc for analysis according to claim 1, wherein the light variability is measured. 14. An enzyme reaction assay is carried out in which the solution to be inspected and the analytical reagent are freely or at a limited site on the substrate, and after substantially completing the reaction, measuring the light variability. The optical disc for analysis according to claim 1, wherein: 15. The antibody-antigen reaction system assay is carried out by freely or at a limited site on the substrate to be inspected solution and the analytical reagent, and after the reaction is substantially completed, the photo-change property is measured. The optical disc for analysis according to claim 1, wherein 16. A nucleotide sequence-antigen reaction system assay is carried out by freely or at a limited site on the substrate to be inspected solution and the analytical reagent, and after the reaction is substantially completed, the photo-change property is measured. The optical disc for analysis according to claim 1, which is measured. 17. An aptamer is contained in the analytical reagent of the nucleotide sequence-antigen reaction system assay,
The optical disc for analysis according to claim 16. 18. A base sequence-base sequence reaction system assay is performed on the substrate freely or at a limited site on the test solution and the analysis reagent, and binds only to the complementary-bonded site. The optical disc for analysis according to claim 1, wherein the light change property is measured after the reaction with the binding reagent is substantially completed. 19. The optical disc for analysis according to claim 18, wherein the binding reagent is an intercalator. 20. The optical disc for analysis according to claim 18, wherein the binding reagent is a labeled antibody. 21. The cell-antibody reaction system assay is performed on the substrate to be tested and the analytical reagent freely or at a limited site, and after the reaction is substantially completed, the photovariability is measured. The optical disc for analysis according to claim 1, wherein: 22. The prokaryote, the eukaryote, or the eukaryotic cell is cultured between the lid and the substrate, and after culturing biological, chemical, molecular biological, or biochemical. One or more reagents or one or more mixed reagents in which any one of them is combined are added through the test solution introducing hole, and after the reaction is substantially completed, centrifugation is performed to determine whether the culture is substantially alive or dead. The optical disc for analysis according to claim 1, wherein the optical disc for analysis is detected by the measurement. 23. The optical disc for analysis according to claim 1, wherein all the holes of the channel filled with the solution are sealed with a lid before being set with the measuring instrument. 24. The analytical optical disk according to claim 1, wherein the analytical optical disk comprises the substrate and the lid, and both or one of them is mainly composed of a polymer having a high thermal solubility. Optical disc. 25. The optical disc for analysis according to claim 1, wherein the optical disc for analysis comprises a substrate and a lid, and both or one of them is produced by a press machine. 26. The optical disc for analysis according to claim 24, wherein the polymer is biodegradable. 27. The silica as a main material of the substrate and / or the lid.
The optical disc for analysis described. 28. The analytical device according to claim 1, wherein one or more positioning elements are provided on the inner circumference and / or the outer circumference of the optical disc for analysis so as to facilitate positioning during manufacturing. Optical disc. 29. The optical disc for analysis according to claim 28, wherein the positioning element is a concave type, a convex type or a combination thereof, and is processed at the time of manufacturing the lid and the substrate. 30. A plurality of channels having different sizes are connected to the chambers in the optical disc for analysis, and the same amount of the same kind of the analytical reagent is provided in the chambers having different sizes. Since the volumes of the chambers having different sizes are different at the time of introducing the test solution, the analytical reagent is diluted with the test solution at different magnifications, and the calibration curve can be easily obtained at the time of analytical measurement after the reaction is completed. 3. The method according to claim 3, wherein
4. The optical disc for analysis according to 4 above. 31. The analysis reagents of the same kind are provided in the branched channels extending in the branched structure having different lengths from the solution introducing holes to be inspected in the disc, respectively, and the inspected sample is inspected. Since the lengths of the branched channels are different at the time of introducing the solution, the analytical reagent is diluted with the test solution at different magnifications, and a calibration curve can be simply obtained at the time of analytical measurement after the reaction is completed. The optical disc for analysis according to claim 3, characterized in that 32. The information portion is a digital signal optical,
The optical disc for analysis according to claim 1, characterized in that the information which substantially covers the information necessary for the analysis is recorded by magnetic or both methods. 33. A rotating shaft capable of holding the optical disc for analysis according to claim 1, a motor for supplying a rotational force to the optical disc for analysis by the rotating shaft, a light source, a light receiving element, and the light source. The optical disc for analysis includes: an operating engine that operates the light receiving element; a circuit that is substantially sufficient for operating the motor, the light source, the light receiving element, and the operating engine to input and output to and from the outside; A measuring instrument characterized by measuring. 34. The measuring instrument according to claim 33, which measures the light variability including light transmissivity, light reflectivity, photothermal conversion property or a combination thereof. 35. The measuring instrument according to claim 33, comprising photoacoustic spectroscopy, thermal lens spectroscopy, photothermal deflection spectroscopy, photothermal reflection spectroscopy, photothermal displacement spectroscopy, photothermal radiation spectroscopy or a combination thereof. . 36. The measuring instrument according to claim 33, further comprising a non-contact type heat source for substantially homogenizing and promoting the reaction of the analytical reagent provided on the optical disc for analysis. 37. The measuring device according to claim 36, wherein the non-contact heat source is one or a combination of a laser, a carbon lamp, an IH heater, a heating wire, and a microwave. 38. The measuring instrument according to claim 33, further comprising a vibration source for substantially homogenizing and promoting a reaction of the analysis reagent provided on the analysis optical disk. 39. The measuring instrument according to claim 33, further comprising an ultraviolet lamp for preventing infection of a measuring person when not operating. 40. The measuring device according to claim 33, further comprising a photocatalyst coating layer for exhibiting a permanent sterilization / sterilization action and preventing infection of a measuring person. 41. The measuring instrument according to claim 33, which is partly manufactured from an antibacterial material for exhibiting a permanent sterilizing / sterilizing action and preventing infection to a measurer. 42. The optical disc for analysis is rotated at a center, and at least one or more light beams scan the optical disc for analysis in a radial direction, and the light variability derived from the optical disc is measured. 34. The measuring device according to claim 33, wherein the measuring operation is advanced based on additional information obtained from the outside of the measuring device in advance. 43. The analysis optical disc is rotated about the center thereof, and at least one or more light beams scan the analysis optical disc in the radial direction, and the light variability derived therefrom is measured. 34. Measuring device according to claim 33, characterized in that the further measuring operation is carried out on the basis of a digital signal obtained in advance from the information part of the analyzing optical disc. 44. The analysis optical disc is rotated at a center, and one or more light beams scan the analysis optical disc in a radial direction to measure the light variability derived therefrom. 34. The measuring device according to claim 33, wherein the measuring operation is advanced based on information obtained from the outside in advance through communication such as the Internet or a mobile phone. 45. The analysis optical disc is rotated about a center, and at least one or more light beams scan the analysis optical disc in a radial direction, and the light variability derived therefrom is measured. 34. The measuring device according to claim 33, wherein the measuring operation is advanced based on information obtained from the outside through a magnetic card in advance. 46. When the information of the measurement operation obtained from the outside in advance cannot be input, the manual operation is externally notified to the measurer, and the measurement operation is advanced by the manual input. Claim 33
The measuring instrument described. 47. At the time of scanning measurement of the optical disc for analysis,
By measuring the light variability in the vicinity of the analysis reagent at a distance that does not substantially affect the light variability with the light source used for the actual scanning measurement, a blank value is indexed and measured. 34. The measuring device according to claim 33, wherein the measuring device reflects the result. 48. The measurement operation including the correction is advanced based on the latest quality control information obtained from a sales company, a manufacturing company, or both through an external communication such as the Internet or a mobile phone before and after the analysis operation. 34. The measuring device according to claim 33, wherein: 49. When the solution to be inspected is blood, a centrifugal force generated by rotating the optical disc for analysis is applied to the solution to be inspected in the optical disc for analysis when measuring.
As a result of the blood cell component by the outer peripheral side in the channel, the hematocrit value can be measured substantially simultaneously with the original analysis item, and the result is added to the calculation process as one of the correction items,
34. The measuring instrument according to claim 33, which contributes to highly accurate quantitative measurement. 50. The apparatus according to claim 1, further comprising a function capable of recording measured values, analysis results, IDs and other items desired by a measurer in the information part of the optical disc for analysis with a measuring instrument. Optical disc for analysis.