JP2002228632A - Gel support vessel for electrophoresis - Google Patents

Abstract

(57) Abstract: An electrophoresis gel in which a pair of opposing spacers provided between two parallel non-conductive flat plates sandwiching a gel and the non-conductive flat plate are adhered to each other. A support container, wherein the adhesive force A between the spacer and the non-conductive plate facing the front surface of the gel and the adhesive force B between the spacer and the non-conductive plate facing the back surface of the gel are different. container. The gel supporting container for electrophoresis of the present invention is easy to assemble, can be manufactured at low cost, has no inconvenience such as peeling during handling operation, and can easily remove the gel after electrophoresis.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a gel support container used for an electrophoresis apparatus.

[0002]

2. Description of the Related Art An electrophoresis method for separating and purifying biological components such as nucleic acids, proteins, and carbohydrates by utilizing the difference in the mobility exhibited by an electric field has been performed using a gel. The spacers and containers that support the gel are made of plastic or glass, and are bonded with an adhesive and then further taped or melt bonded. For this reason, when removing the gel from the support container after the electrophoresis, the gel has to be peeled off with a strong force, which has been a factor of breakage of the gel and injuries of hands and fingers. Also, in order to avoid such inconvenience, the gel may be manufactured in a state where the spacer is not fixed to the container supporting the gel, but in actual use, the gel and the support container are displaced and become unusable, In some cases, air bubbles were introduced and the original performance could not be exhibited or new inconveniences occurred. As a method of easily taking out the gel from the gel supporting container, a spacer is provided inside the side edge of the non-conductive plate, a space between the side edge and the spacer is provided with a soft-curing adhesive, and the adhesive is attached with a pull tab. Method for tearing out the agent and removing the gel (Japanese Patent Laid-Open No. Hei 6-21386)
No. 5) has been proposed.

[0003]

However, the gel-supporting container used in these electrophoresis is difficult to assemble, so that the cost is high, and those easily assembled without bonding are not easily separated during handling. Inconvenience occurs. Further, a strong force may be required when removing the gel after electrophoresis, or safety work may not be possible, and a gel support container that can be operated easily and safely at low cost is desired.

An object of the present invention is to provide a gel support container for electrophoresis which is easy to assemble, can be manufactured at low cost, has no inconvenience such as peeling during handling operation, and can easily remove the gel after the electrophoresis operation. It is in.

[0005]

The present inventor has solved these problems by bonding the spacer of the gel support container for electrophoresis to a non-conductive plate holding the gel with adhesives having different adhesive strengths. The present invention has been completed by finding a solution.

According to the present invention, there is provided a pair of opposing spacers provided between two parallel non-conductive flat plates sandwiching a gel,
What is claimed is: 1. A gel supporting container for electrophoresis in which an adhesive force between said non-conductive plate and said non-conductive plate is adhered to each other. An object of the present invention is to provide a gel support container for electrophoresis in which the adhesive force B between the flexible plate and the spacer is different. Also,
An object of the present invention is to provide an electrophoresis cassette in which a gel is filled in the electrophoresis gel support container.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The gel supporting container for electrophoresis of the present invention comprises two parallel non-conductive plates for holding the gel and spacers provided opposite to the left and right side edges of the plate. Container.

The material of the non-conductive flat plate used in the present invention is
Glass or plastic such as polyethylene, polyacrylate, polyvinyl chloride and the like can be mentioned. The non-conductive flat plate may have a different size or shape, or may have been subjected to processing such as printing so that the front and rear surfaces can be easily distinguished.

The material of the spacer used in the present invention is non-conductive, and may be the same material as the non-conductive flat plate.

The adhesive force A between the front non-conductive plate and the spacer and the adhesive force B between the rear non-conductive plate and the spacer of the gel supporting container for electrophoresis of the present invention are 0.01 to 0.01.
It is preferably 10 N / cm, particularly preferably 0.1 to 10 N / cm. In addition, the difference between the adhesive strength A and the adhesive strength B is at least 0.
It is preferably at least 01 N / cm, more preferably 0.01 to 10 N / cm, and particularly preferably 0.05 to 5 N / cm, since the removal of the gel is extremely simple.

[0011] Examples of the adhesive having an adhesive force between the nonconductive flat plate and the spacer of 0.01 to 10 N / cm include acrylic, rubber, and vinyl adhesives. Agents can also be used.

The bonding between the nonconductive flat plate and the spacer is performed by using the above-mentioned adhesive. However, using such an adhesive as a double-sided adhesive tape coated on both sides of the tape further increases the assembly. Simple and easy to remove the gel is preferred. As the tape used at this time, a commercially available tape using polypropylene, polyester, or the like as a base material is preferable. By using such double-sided adhesive tape,
In order to make the adhesive strength A and the adhesive strength B different, tapes having an adhesive strength in the above range may be used, but the same double-sided adhesive tape is used, and the adhesive strength is adjusted by changing the size. Is preferred.

The size of the electrophoresis gel supporting container of the present invention can be variously adjusted and is not particularly specified.

[0014]

DESCRIPTION OF THE PREFERRED EMBODIMENTS An electrophoresis gel supporting container manufactured as an example of the present invention will be described below with reference to the drawings. The present invention is not limited to the gel support container for electrophoresis shown in this drawing.

FIG. 1 is a perspective view of an electrophoresis gel supporting container of the present invention, and FIG. 2 is a side view thereof. The gel support container for electrophoresis in FIG. 1 is a front flat plate 1 having a notch 4 at the top made of glass having a length of 10 cm, a width of 10 cm and a thickness of 0.1 cm.
0cm, 10cm wide, 0.1cm thick glass rear flat plate 2
Between them, an acrylic spacer 3 having a length of 10 cm, a width of 1 cm and a thickness of 0.1 cm is adhered and arranged via a double-sided adhesive tape. This double-sided adhesive tape is obtained by applying an acrylic adhesive to a polyester film as a base material. The adhesive strength A was 0.2 N / cm, and the adhesive strength B was 4.3 N / cm.
cm. FIG. 2 is a side view of the electrophoresis gel supporting container, wherein the front flat plate 1 and the rear flat plate 2
Indicates that they are joined via

The gel used for the electrophoresis measurement includes a front flat plate 1, a rear flat plate 2, and a pair of opposing spacers 3,
3 ′. An acrylamide solution prepared by an ordinary method is injected into the space and polymerized to produce a polyacrylamide gel. For the injection of the acrylamide solution, there is a method in which one or a plurality of gel supporting containers are put in a box-shaped container and the liquid is injected into the container. Next, a protein sample or the like is applied to the gel according to a conventional method to perform electrophoresis, and after completion, a flat object such as a spatula is inserted into the lower end of the gel support container, and the front flat plate 1 is removed.
The polyacrylamide gel attached to the rear surface is peeled off, and operations such as staining are performed.

[0017]

The gel supporting container for electrophoresis of the present invention is easy to assemble, can be inexpensive, has no inconvenience such as peeling during handling operation, and can easily remove the gel after electrophoresis.

[Brief description of the drawings]

FIG. 1 is a perspective view of a gel support container for electrophoresis of the present invention.

FIG. 2 is a side view thereof.

Claims (3)

[Claims] 1. A gel supporting container for electrophoresis wherein a pair of opposing spacers provided between two parallel non-conductive plates for holding a gel and a non-conductive plate are adhered to each other. An electrophoresis gel support container in which the adhesive force A between the spacer and the non-conductive flat plate facing the front surface of the gel is different from the adhesive force B between the non-conductive flat plate and the spacer facing the rear surface of the gel. 2. Both of the adhesive strengths A and B are 0.01 to
10 N / cm and the difference is at least 0.01
2. The gel support container for electrophoresis according to claim 1, wherein the density is not less than N / cm. 3. An electrophoresis cassette in which a gel is filled in the electrophoresis gel support container according to claim 1.