JP2002153269A - Method for stabilizing protease solution and skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for stabilizing a protease solution to reduce the lowering of titer even in storage for a long period. SOLUTION: This method for stabilizing the protease solution is characterized by formulating fermented Curcuma aromatica and/or Curcuma longa and a polyhydric alcohol.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a method for stabilizing a protease solution and a skin cosmetic.

[0002]

2. Description of the Related Art Normally, keratinocytes of the skin are peeled off as dirt from the surface of the skin sequentially from the old ones following the turnover of the epidermis. This phenomenon is called desquamation, but if it is delayed due to aging or dryness, old keratinocytes that originally peel off will accumulate on the skin surface, losing the transparency of the skin, and various cosmetic problems such as dark spots, spots and rough skin will occur. Occurs.
Further, if this desquamation delay occurs in the hair follicle, the hair follicle becomes narrow, and finally, it becomes blocked and acne develops. In order to solve these problems, α-hydroxy acids such as glycolic acid and lactic acid and salicylic acid have been used for the purpose of exfoliating old keratinocytes deposited on the epidermis.
It is said that it is necessary to mix at a high concentration of 12% or 30 to 70%, and irritability, photosensitivity, and promotion of pigmentation are problems.

[0003] Therefore, focusing on the fact that a certain protease resident in the skin degrades the adhesion protein and desmosome to cause desquamation, Altea, Yukinoshita and apricot are used as components for promoting the action of these enzymes. Plant extracts such as extracts have begun to be used. However, when the production amount of these indigenous enzymes is extremely small or nonexistent due to external factors such as ultraviolet rays, chemical substances, drying, and internal factors such as physiological aging, and also due to individual differences and site differences. It is self-evident that the desired effect cannot be obtained.

[0004] The present inventors considered that in order to solve this demerit, it would be expedient to directly promote desquamation by adding a protease such as a plant or microbial substrate to cosmetics.
Protease is mild in action and has the advantage of removing excess keratinocytes without putting a burden on the skin.However, it is currently used in cosmetics, and its use is extremely narrow. Limited. The reason is that the protease solution is unstable. Therefore, in order to apply the above idea to a wide range of cosmetics, there was a difficult problem that an inherently unstable protease solution had to be stabilized.

[0005] One of the present inventors has previously proposed a method of blending a water-soluble organic and / or inorganic calcium salt with a polyhydric alcohol in order to stabilize a protease solution (Japanese Patent Publication No. 37-16696). Furthermore, a method using a monohydric alcohol in combination (Japanese Patent Publication No. 45-30192) was developed. However, water-soluble calcium salts are unsuitable as cosmetic ingredients such as impairing the smoothness of the skin, so that they have been applied to the production of cosmetics. I rarely did.

[0006]

The problem to be solved by the present invention is to solve the above-mentioned drawbacks of the prior art, to stabilize a protease solution with a small decrease in titer even when stored for a long period of time, and to reduce keratin of the epidermis. An object of the present invention is to provide a skin cosmetic which promotes desquamation of cells and has an effect of preventing and treating dark spots, spots, rough skin and acne.

[0007]

Means for Solving the Problems The inventors of the present invention, during close examination of data on known botanical cosmetic ingredients, focused on the fact that there was no case where a fermentation step was performed in the extraction step of the ingredients, and the fermentation was carried out. The stabilization of protease solution for plant cosmetic ingredients was studied diligently. As a result, they have found that the above problems can be solved by using fermented turmeric in combination with a specific compound, and have completed the present invention.

That is, the present invention provides (1) a method for stabilizing a protease solution, which comprises mixing fermented turmeric and a polyhydric alcohol, and (2) a method further comprising mixing a monohydric alcohol. (3) a method for stabilizing a protease solution according to (3), a skin cosmetic comprising a protease solution, fermented turmeric, and a polyhydric alcohol as essential components; and (4) further containing a monohydric alcohol. A skin cosmetic according to (3), which is characterized by the following.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The protease used in the present invention is a microorganism-based protease such as Bacillus subtilis and Streptomyces.
One or more enzymes produced by griseous) can be appropriately selected and used.

[0010] The protease solution in the present invention is not particularly limited as long as it is a solution containing a protease. For example, the above-mentioned microbial substrate protease powder is dissolved in water or a pH buffer.

In the present invention, fermented turmeric, which is one of the essential components for stabilizing the protease solution, refers to monascus anchor (Mocurus aurora) and / or turmeric (Curucuma longa).
nascus anka), Monascus pirousus (Monascus pi)
loas), Monascus ruber, etc., and is a powder obtained by fermentation by appropriately selecting and using at least one filamentous fungus of the genus Monascus (Aspergillus oryzae). In the present invention, this powder may be used as it is, or an extract with a hydrophilic solvent such as water, ethanol, and 1,3-butylene glycol may be used.

In the present invention, the polyhydric alcohol, which is another essential component for stabilizing the protease solution, is not particularly limited as long as it has two or more hydroxy groups in one molecule. Examples thereof include propylene glycol, 1,3-butylene glycol, sorbitol, diglycerin, and dipropylene glycol, and these may be used alone or in combination of two or more.

The present invention aims to stabilize a protease solution by using fermented turmeric in combination with a polyhydric alcohol.
By blending a monohydric alcohol in addition to the above essential components, the protease solution can be stabilized with a smaller amount of the added polyhydric alcohol. As the monohydric alcohol, for example, one or more kinds such as ethanol and isopropyl alcohol can be used.

The amount of these components is 1 [PU] to 1000 [PU], preferably 10 [PU] to 300 [PU] per g or ml of protease. The fermented turmeric content is 0.5 to 30% by weight, preferably 1 to 10% by weight. The polyhydric alcohol accounts for 1 to 90% by weight, preferably 5 to 7% by weight. The monohydric alcohol is 1 to 30% by weight, preferably 5 to 15% by weight.

The skin cosmetic of the present invention may contain any of the above-mentioned components, and can be widely applied to solutions, emulsions, gels, and the like. May be blended.

[0016]

EXAMPLES Example 1, Comparative Examples 1 and 2 The mixed solutions shown in Table 1 below were prepared, and Bacillus subtilis protease was added to each so as to give 50 [PU] per ml, and stored at 35 ° C. .

The protease titer was ^{expressed as} [PU] ^Cas.Fr.B γ _tyr (hereinafter abbreviated as [PU]) according to the general test method for protease ^varieties and the second method of protease titer test.

Fermented turmeric was prepared according to the following method.

That is, the rhizome of turmeric (Curucuma longa) is washed with water, sliced, dried by heating until the water content becomes about 10%, and then pulverized until the average particle diameter becomes 2 to 4 mm to obtain dried turmeric powder. Obtained. 5 g of rice bran, 0.5 g of magnesium sulfate and 10 g of glucose are thoroughly mixed with 100 g of the powder, and then mixed until the water content reaches 60%.
Hot water at 0 ° C. was added. This mixture is subjected to a pressure sterilization treatment (12
0 ° C., 1 hour, 1.1 atm), cooled, and inoculated with Monascus anchor, previously cultured in a liquid medium.
Fermentation was carried out for 30 days under the conditions of 5 to 30 ° C and a water content of 60%. The obtained fermented product was dried by heating (70 to 80 ° C., 8 hours) and pulverized. The above method for producing fermented turmeric is an example, and the present invention is not limited to this.

[0020]

[Table 1]

After each mixture was stored at 35 ° C., the titer of the protease was measured, and the titer preservation ratio was determined. One month later, the mixture of Comparative Example 1 had a potency preservation rate of 42% and Comparative Example 2
It was 31%. On the other hand, the potency preservation rate of the mixture of Example 1 was 100% after 3 months. Therefore, it is understood that the stability of the protease solution is improved by the synergistic effect of the fermented turmeric and the polyhydric alcohol.

Comparative Examples 3 and 4 Bacillus subtilis protease was added to each of 50 [PU] per ml.
, And a mixture as shown in Table 2 below was prepared, and the protease titer was measured in the same manner as in Example 1. The unfermented turmeric is one obtained from the above fermented turmeric production method without adding Monascus anchor.

[0023]

[Table 2]

The preservation solution in Table 2 was stored at 35 ° C. for one month, and the protease titer was measured to determine the residual ratio of the protease.
The mixture of Comparative Example 3 had a potency preservation rate of 6% and Comparative Example 4
It was 30%. Therefore, in unfermented turmeric,
No stabilizing effect of the protease solution was observed.

Example 2 A mixture shown in Table 3 below was prepared and stored at 35 ° C.

[0026]

[Table 3]

When the residual titer after one month was determined, it was 81% in Example 2.

As is clear from the results shown in Table 3, the method for stabilizing a protease solution of the present invention is effective not only for Bacillus subtilis protease solution but also for actinomycete protease solution. In addition, it can be seen that by using a polyhydric alcohol and a monohydric alcohol together, an effect of stabilizing the protease solution can be obtained even when the amount of the polyhydric alcohol added is reduced.

Example 3 A skin cosmetic (lotion) was prepared according to a recipe shown in Table 4 below by a conventional method. The residual potency of the obtained lotion was 82% at room temperature for 3 months.

[0030]

[Table 4]

Note *: 5 g of fermented turmeric powder was purified water 50
g, ethanol 10 g and propylene glycol 20
g of the mixed solution, and the mixture was stirred and mixed.

(Skin Improvement Effect) The above-mentioned lotion was used twice daily in the morning and evening for three months by 30 women aged 18 to 55 years. Evaluation of effectiveness was self-reported by questionnaire. As a result, in the evaluation items of lightening of spots, lowering of consciousness of dark spots, and whitening of the skin, 21 persons answered that "slightly improved" or more. In addition, in the evaluation items for the reduction of acne and the reduction of pigmentation after acne, 14 persons answered that the evaluation was “slightly improved” or more.

Example 4 A skin cosmetic (cream) was prepared by a conventional method according to the formulation shown in Table 5 below.

[0034]

[Table 5]

Example 5 A skin cosmetic (face pack) was prepared by a conventional method according to the formulation examples shown in Table 6 below.

[0036]

[Table 6]

[0037]

Industrial Applicability The method for stabilizing a protease solution of the present invention is an excellent method in which the titer of the protease solution is less reduced even when stored for a long period of time, and the production of skin cosmetics having an effect of promoting desquamation of keratinocytes. Suitable for.

The skin cosmetic of the present invention promotes desquamation of keratinocytes of the epidermis and prevents dark spots, spots, rough skin and acne.
It exerts a therapeutic effect, and the protease titer rarely decreases even after long-term storage.

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Claims (4)

[Claims] 1. A method for stabilizing a protease solution, comprising blending fermented turmeric and a polyhydric alcohol. 2. The method for stabilizing a protease solution according to claim 1, further comprising blending a monohydric alcohol. 3. A skin cosmetic comprising a protease solution, fermented turmeric and a polyhydric alcohol as essential components. 4. The skin cosmetic according to claim 3, further comprising a monohydric alcohol.