JP2002097153A - Composition containing glutathione-containing yeast extract - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a composition capable of masking an unpleasant taste and a smell of a yeast extract containing glutathione, and comprising the extract administrable after dissolution in the mouth. SOLUTION: This method for producing the composition containing a yeast essence containing glutathione comprises a process for obtaining a solidified substance by compression molding yeast essence powder containing glutathione under an anhydrous condition, a process for grinding and granulating the obtained solidified substance and a process for coating the granule obtained by the grinding with a nonaqueous coating agent. This composition is obtained by the method.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention provides a composition which masks the unpleasant taste and odor of a yeast extract component containing glutathione, and allows the extract component to be dissolved in the mouth and taken.

[0002]

2. Description of the Related Art Glutathione as a medical or pharmaceutical raw material has been used as a therapeutic agent for enhancing immunity and improving liver dysfunction for a long time. Glutathione is known to have a wide range of medicinal effects. For example, it activates the metabolism of fat, protein, and sugar, and exhibits effects such as detoxification, liver function improvement, fatty liver improvement, and antiallergy. Glutathione is used for the treatment and prevention of various conditions and diseases, such as drug poisoning, acetone-induced vomiting (periodic poisoning, periodic vomiting), metal poisoning, pregnancy vomiting, and blurred vision. I have.

[0003] Glutathione is known to be widely distributed in animal and plant tissues and microorganisms. Generally, glutathione preparations are produced from yeast. recent years,
High-concentration yeast before purification can be handled as health food. However, glutathione-producing yeast cells and extracts thereof have an unpleasant odor and taste and cannot be eaten as it is.

In particular, concentrated yeast extract has a high glutathione content and is expected to have a high pharmacological effect. However, since it has a bad odor and is extremely unpleasant, it does not dissolve in the oral cavity for oral ingestion. There was no choice but to use the internal medicine as described above.

[0005] In the case of oral preparations and drinks, it takes time for the blood concentration to rise because it is absorbed by the intestinal wall. on the other hand,
For example, when it is desired to act quickly after taking the drug, for example, to improve the symptoms of intoxication caused by drinking alcohol, ie, to get sick, hangover, or impaired liver function, it is necessary to shorten the absorption time from ingestion. For this purpose, it is desirable to gradually dissolve or disintegrate in the mouth to prepare a preparation called a troche or chewable type which is absorbed under the tongue.

[0006]

An object of the present invention is to provide a composition which masks the unpleasant odor and taste of the glutathione-containing yeast extract and which can be dissolved and taken in the mouth.

[0007]

A step of obtaining a solidified product by compressing and molding a yeast extract powder containing glutathione under anhydrous conditions; a step of crushing and granulating the obtained solidified product;
A method for producing a composition containing a glutathione-containing yeast extract, comprising a step of coating particles obtained by crushing with a coating agent.

In the method of the present invention, the hardness of the solidified product obtained by compression molding is preferably 10 kg or more, and the average particle size of the particles obtained by crushing the solidified product is preferably between 24 and 80 mesh.

[0009] The particles obtained by compression, crushing and classification are then coated with a non-aqueous coating agent.

The composition of the present invention obtained by coating with a non-aqueous coating agent is compression-molded into an appropriate shape together with excipients and other additives, and is gradually dissolved in the mouth to be taken. It is provided as chewable tablets, troches and the like. The composition of the present invention which can be taken by dissolving in the mouth can be taken quickly after the ingestion because it is absorbed from under the tongue, and is particularly suitable when a rapid action such as a disorder due to a large intake of alcohol is desired. Used for

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The yeast extract containing glutathione used in the present invention is obtained by a known method from cells obtained by culturing baker's yeast, alcohol-fermenting yeast and the like by a known method.

[0012] The yeast extract powder used in the present invention is obtained by concentrating the obtained extract, adding an excipient, and drying to obtain a powder product. Examples of the excipient include dextrin, gelatin, sodium caseinate, lactalbumin and egg white.

[0013] Commercially available yeast extract powder is readily available. The yeast extract powder contains glutathione 8 to 1
It is preferable to use a substance containing 0%. Commercially available yeast extract powders, for example, Torula yeast extract having a glutathione content of 8% are particularly preferably used.

In the method of the present invention, the yeast extract powder is compression-molded in an appropriate mold. The molding may be carried out using a tableting device usually used for producing tablets of medicines and dietary supplements. In molding, it is preferable to add a lubricant usually used in the preparation, for example, a sucrose fatty acid ester in order to prevent the material from adhering to the punch.

The solidified product obtained by the compression molding step is
Preferably hardness 10 kg or more, more preferably 14 kg
The above tablets are used. There is no particular upper limit for the hardness, but 17.5.
The hardness may be not less than kg.

The pressure at the time of molding is not particularly limited as long as a desired hardness can be obtained.
00kg / cm ² or more, and particularly preferably 1700 kg / cm ² or more. There is no particular upper limit for the pressure during molding,
For example, tableting may be performed at a pressure of 3000 kg / cm ² or more.

The solidified product having the desired hardness is then crushed. The crushing may be performed using a device usually used in the production of a pharmaceutical. Classify the particles obtained by crushing,
Particles having a particle size of 0 to 90 mesh, preferably 24 to 80 mesh are obtained.

The particles obtained are then coated with a non-aqueous coating. The coating may be performed by first coating the alcohol-soluble substance with an alcohol solution, and then further coating with a powder coating agent. Coating may be carried out using any of the devices usually used for coating granules and the like in the production of pharmaceuticals and dietary supplements. For example, when a sugar-coated bread is used, the solidified product is pulverized, the particles obtained by classification are put into a sugar-coated bread, and an alcohol solution of a coating agent is sprayed thereon to perform a coating treatment. It should be processed.

As the alcohol-soluble non-aqueous coating agent, any of the materials usually used in the manufacture of pharmaceuticals and dietary supplements can be suitably used. For example, shellac, hydroxyethyl cellulose, hydroxypropyl starch, hydroxypropyl Cellulose, hemylose and the like are exemplified.

After coating with an alcohol-soluble substance, talc, boray including oyster shells, zinc oxide, aluminum oxide, calcium oxide, titanium oxide, propylene oxide, magnesium oxide, potassium carbonate, calcium carbonate, sodium hydrogen carbonate, carbonate A coating in powder form, selected from the group consisting of sodium, magnesium carbonate, lithium carbonate, precipitated calcium carbonate and natural aluminum silicate is applied. By applying such a powdery coating agent, particles that become sticky only by coating with the alcohol-soluble substance are not sticky, and the taste and smell masking effects of the yeast extract are further increased.

After the coating with the powdery coating agent is completed, it is dried. The coating with the non-aqueous coating agent may be performed only once, or the coating with an alcohol-soluble substance, the coating with a powdery substance, and the drying may be repeated twice or more. Preferably, the coating step is 2
That is, to form a plurality of coating layers by repeating the coating more than once, thereby enhancing the taste and smell masking effect. In the drying step, the temperature is lower than 50 ° C, preferably lower than 40 ° C. If the temperature exceeds 50 ° C., glutathione is decomposed and deteriorated.

The coating amount of the coating agent may be appropriately selected depending on the type of the coating agent. For example, in one embodiment, when an alcoholic solution of purified shellac and talc are used as the non-aqueous coating agent and are coated in two stages, a base coat and a finish coat, the first base coat contains 0.5 g / ml purified shellac. After coating the ethanol solution by spraying about 26% by weight of the ground particles, the sticky surface is treated with talc. The amount of talc is not limited, but may be about 24 to 25% by weight of the crushed particles. After completion of the undercoat, the particles are dried once,
Finish coating is applied. The preferred composition is obtained by coating the same concentration of ethanol solution in the finish coat with about 7% by weight of the ground particles and further treating with 6-7% by weight of talc.

The coated particles obtained according to the present invention are sufficiently masked for the unpleasant taste and odor of the glutathione-containing yeast extract and can be taken while dissolving or disintegrating in the mouth. The coated particles may be classified as needed, further processed, or taken as is.

The composition of the present invention thus obtained can be used as a glutathione-containing particle for nutritional supplements and pharmaceuticals. Even if it is orally administered as a granule,
Further, it may be added to a preparation for oral administration containing other components or a food.

The coated particles obtained by the method of the present invention may be further subjected to compression molding together with appropriate excipients and other additives to produce a tablet to be dissolved in the mouth and taken. Such compositions for absorbing components while dissolving in the mouth are also included in the scope of the present invention.

In preparing a composition to be dissolved and taken in the mouth, the excipients and other additives to be added to the coated particles obtained according to the present invention are usually those in the field of dietary supplements and pharmaceuticals. Any known products known to be used when producing troches, chewables, and the like to be dissolved in the mouth may be used as appropriate. Examples of the additive include a non-aqueous binder, a disintegrant, a sweetener, a flavor, a preservative, and the like. The composition to be dissolved in the mouth may be produced in accordance with a conventionally known method for producing a troche or chewable. Briefly, excipients and other additives may be mixed with the coated particles of the present invention and compressed with a suitable tableting device. The shape and physical properties of the tablet are not particularly limited, and may be appropriately selected according to the purpose.

The composition obtained according to the present invention is sufficiently masked with the unpleasant taste and odor of the yeast extract, which has conventionally been a problem. Therefore, according to the present invention, glutathione can be dissolved in the mouth and provided as a preparation that can be absorbed under the tongue.

Hereinafter, the present invention will be described in more detail with reference to examples.

Example 1 The production conditions of the composition were examined using Torula yeast extract (a glutathione-containing yeast extract) YH1 (purchased from Kojin Co., Ltd.). The yeast extract powder used was
It contained 8.9% by weight of glutathione. 6000 g yeast extract powder and 180 g sucrose fatty acid ester
Was charged into a mixer and mixed for 3 minutes. Using a 10.5 mm flat R punch, the weight of one tablet was set to 300 mg and the tableting pressure was set to 500 to 3000 k using a Libra 836 KAWCZ type tableting machine (Kikusui Seisakusho).
g / cm ² to obtain a solid. The hardness of the obtained solid was examined. Table 1 shows the results.

[0029]

[Table 1]

Of the solids shown in Table 1, A and B
Was not used for the next stage because cracking and chipping were observed. Each of the obtained C to H tablets was pulverized and classified. Roll type crusher RC212 (Kikusui Seisakusho) for crushing
Was used. After pulverization, the obtained particles were sieved and classified. Classification was performed using an automatic sieve (Koei Sangyo). Classification is performed when particles are 10 to 14, 14 to 24, 24 to 50, and 5 respectively.
It was divided into particles of 0-80, 80-100 and 100-200 mesh.

The obtained particles having the respective particle diameters were coated with an ethanol solution of shellac obtained by mixing 5000 g of purified shellac with 10,000 g of ethanol and mixing for 2 hours with a ball mill. For coating, sugar-coated bread type 603 (Iwakuro Seisakusho) was used, and the classified crushed particles were added thereto. A coating treatment was performed by spraying a shellac alcohol solution of 26% by weight based on the weight of the pulverized particles. Next, talc powder was added to the sugar-coated bread and coated. At the point when the sticky surface was evenly coated, 24.27% by weight of talc was used based on the ground particles.

After the coating, the particles were once taken out, dried at 38 ° C. for 24 hours, and again subjected to a finish coating with an ethanol solution of shellac and talc in the same manner as described above. In this finish coating, 7
A weight percent solution of shellac in ethanol was sprayed on and then talc was added to further form a film. The amount of talc used was 6.12% by weight based on the ground particles when the film was uniformly coated with talc and the odor was reduced. After the completion of the coating treatment, the coating was dried at 38 ° C. for 24 hours to obtain coated particles.

Using the obtained coated particles, tablets were prepared for dissolution in the mouth and for taking. The following components: coated particles 8908 g Vitamin C 4044 g Reduced maltose 3600 g Riboflavin 8 g is mixed in a V-type mixer VM-200 (Fuji Paudal), and then Tindome Gran TDG-1
The mixture was granulated at 10 (Fuji Paudal) and dried at 38 ° C.

Further, 540 g of orange micron (perfume) and 900 g of sucrose fatty acid ester (lubricant) were added to the obtained granules, and mixed with a V-type mixer VM-200 (Fuji Paudal). The resulting mixture was tableted using Collect 21Kr (Kikusui Seisakusho). Tableting was carried out under the following conditions: punch used: 16 mm flat R tablet weight: 1800 mg tableting pressure: 850 to 1000 kg tablet hardness: 5 to 8 kg The surface was cleaned to obtain the tablet of Example 1. Each of the obtained tablets has a diameter of 1
It is a flat lozenge with a pale yellow diameter of 16 mm and a thickness of about 6 mm and a tablet weight of about 1800 mg. The obtained tablet was contained in the mouth, and it was evaluated whether or not the tablet could be dissolved in the mouth and taken based on the taste and smell. Table 2 shows the results.

[0035]

[Table 2]

Only particles having a particle size of 24-80 mesh before coating had a taste and odor that could be dissolved in the mouth and taken. However, even with this particle size, if the hardness of the solidified product before the crushing treatment was 8.5 kg or less, the solidified product was dissolved in the treatment step due to insufficient hardness.

[0037]

Example 2 Using the same components and equipment as used in Example 1, the pressure during the compression molding of the yeast extract powder was set to 110
A solidified product was prepared at 0 kg / cm ² , which was pulverized and classified into 24-80 mesh particles.

The obtained particles were put in the same sugar-coated bread as in Example 1, and the same amount of purified shellac as in Example 1 was sprayed with the same amount of ethanol solution as in Example 1 and coated as a base coat. After adding 735 g of oyster shell powder,
Further, 735 g of talc was added for coating. The obtained particles were dried at 38 ° C. for 24 hours and then coated with the same amount of a shellac ethanol solution as in Example 1 as a final coating, followed by 245 g of oyster shell powder and talc 2
45 g were put into sugar-coated bread and coated. The resulting coated particles were dried at 38 ° C. for 24 hours and used for the next step.

The obtained coated particles were molded in the same manner as in Example 1 with the following formulation to obtain a tablet of Example 2. Formulation: coated particles 8656 g vitamin C 3930 g reduced maltose 3500 g riboflavin 7 g fragrance 522 g sucrose fatty acid ester 875 g Total 17490 g Finish 17490 g

The amount of glutathione in the obtained tablets was measured. In addition, the compounding quantity of each component per tablet is as follows:

[Table 3]

The amount of glutathione was measured by a conventional method.
Since the glutathione content in the yeast extract powder is 8.9%, the theoretical value is 600 mg × 8.9% = 53.
4 mg, but the found in the final product was 50.7 mg.

[0042]

Example 3 The tablet (A) produced in Example 2 and the materials listed in Table 4 as comparative examples:

[Table 4]

Were mixed and tableted in the same manner as in Example 1 to produce a tablet (B). 26 subjects, 11 men
Names (20-55 years old) and 15 women (22-50 years old) licked each tablet in their mouth. The duration, taste and odor that could be included in the mouth were evaluated.

As a result of this test, all the subjects evaluated that the tablet (A) of the present invention could be licked until the tablet was completely dissolved in the mouth, tasted good, and odor was not noticeable. On the other hand, the tablet (B) obtained by compression-molding the yeast extract powder together with excipients and the like was evaluated as unsatisfactory enough that all the test subjects could not stand it as soon as they were put into their mouths, and also unsatisfactory with regard to odor.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 47/02 A61K 47/04 47/04 47/30 47/30 47/38 47/38 A61P 1/16 // A61P 1/16 3/06 3/06 37/08 37/08 39/02 39/02 A61K 37/02 F term (reference) 4B018 LE01 LE02 MD01 MD03 MD04 MD20 MD34 MD35 MD81 ME14 MF01 MF07 MF08 4C076 AA44 BB01 DD21H DD25H DD27H DD28H DD29H EE30H EE31H EE32H EE58H FF06 FF52 FF68 GG16 4C084 AA03 BA15 CA05 MA05 MA35 MA52 NA09 ZA711 ZA712 ZA751 ZA752 ZB131 ZB132 ZC331 ZC332 ZC371 ZC3724A037C

Claims (8)

[Claims] 1. A step of obtaining a solidified product by compression-molding a yeast extract powder containing glutathione under anhydrous conditions, a step of crushing and granulating the obtained solidified product, A method for producing a composition containing a glutathione-containing yeast extract, comprising a step of coating with a coating agent. 2. The solidified product has a hardness of 10 kg / cm ² or more, and the particles obtained by crushing have an average particle size of 24 to
The method of claim 1, wherein the method is 80 mesh. 3. The non-aqueous coating agent comprises shellac,
Alcohol solution of a substance selected from the group consisting of hydroxyethylcellulose, hydroxyethylstarch, hydroxypropylcellulose, and hemylose, and talc, oyster hulls, zinc oxide, aluminum oxide, calcium oxide, titanium oxide, propylene oxide, The method according to claim 1 or 2, comprising a powder selected from the group consisting of magnesium oxide, potassium carbonate, calcium carbonate, sodium bicarbonate, sodium carbonate, magnesium carbonate, lithium carbonate, precipitated calcium carbonate and natural aluminum silicate. 4. The method according to claim 3, wherein the non-aqueous coating comprises an alcoholic solution of shellac and talc and / or oyster powder. 5. The method of claim 1, further comprising the step of compression molding the resulting coated particles with excipients and other additives to form a formulation for dissolution in the mouth for administration. 6. The yeast extract powder contains glutathione 8 to
The method according to any one of claims 1 to 5, wherein the content is 10%. 7. A glutathione-containing yeast extract powder is obtained by compression molding under anhydrous conditions to obtain a solidified product, crushing the obtained solidified product, and coating the particles obtained by the crushing with a non-aqueous coating agent. A composition comprising a glutathione-containing yeast extract. 8. The composition according to claim 7, which is intended to be dissolved in the mouth and taken.