JP2001321364A - Blood taking and separating apparatus by self- exsanguination and serum collecting method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an extremely compact and convenient blood taking and separating apparatus for self-exsanguination. SOLUTION: This blood taking and separating apparatus by self exsanguination comprises a vessel for housing the taken blood, a means for taking the serum eluted by the natural coagulation of the blood, and a vessel for automatically housing the separated and collected serum.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an instrument for separating a very small amount of blood collected by self-blood sampling and a method for collecting serum.

[0002]

2. Description of the Related Art In recent years,
The increase in lifestyle-related diseases is a problem. Lifestyle-related diseases are a group of diseases in which lifestyle habits such as eating habits, exercise habits, rest, smoking, and drinking are considered to be involved in the onset and progression of the disease, such as cancer, diabetes, and stroke. There is. These lifestyle diseases are not easy to treat after onset. For this reason, it is important to prevent the onset of the disease by primary prevention of lifestyle-related diseases, that is, by managing one's own health.

It is said that lifestyle-related diseases can be avoided by performing primary prevention and improving lifestyle. However, even when trying to improve lifestyle without subjective symptoms, individual knowledge often does not provide guidance on how to specifically improve. For this reason, it is effective to work on primary prevention after receiving appropriate advice from specialists such as doctors in order to avoid lifestyle-related diseases or delay the progress.

[0004] However, there is the following problem when receiving the advice of an expert. First, it is difficult for a person seeking advice to make time for receiving advice. Second, there are many experts who need advice, so it is not always possible to secure enough time to give advice to each person and the quality of advice may not be maintained. Since many of the people who give advice are healthy people, they will give advice to a very large number of people, but the system for giving advice to such a large number of people is not well established.

In response to this situation, the present inventor has disclosed in Japanese Patent Application No. Hei.
No. 1-352399, "(1) A medium for storing health information in response to a user's application (a life questionnaire sheet in which questions are described and answers to the question are recorded by the user, Mailing the user with a blood collection needle and a kit for storing the dropped blood)
(2) receiving the medium in which the health information is stored by the user; (3) extracting the user's health information stored in the medium and inputting it to the pathological analysis expert system; and (4) the pathological analysis expert. (5) the system creates advice on the user's health;
Reporting the user's health advice created by the pathological condition analysis expert system to the user. "Suggested. According to such a method, it is possible to prevent lifestyle-related diseases while staying at home without having to go to a medical institution or a specialized institution for medical examination.

The present invention relates to an improvement of a "kit for storing dropped blood" used in the above method.

That is, a conventional blood test is performed by a user going to a medical institution or the like and collecting blood there. Generally, plasma and serum are separated from blood in clinical tests (especially sample tests) by centrifugal separators in clinical laboratories such as medical institutions, or facilities or laboratories that are licensed by a registered hygiene laboratory. It is separated into formed components (blood cell portion) and plasma or serum, and is subjected to a test.

However, a series of separation operations using a centrifugal separator from blood collection in the above-mentioned current method is very complicated,
It does not lead to a reduction in operational efficiency or cost.

[0009]

In view of the above, the present inventor has developed an extremely compact blood collection / separation apparatus which can collect blood and separate and collect serum in the above-mentioned health advice method. ADVANTAGE OF THE INVENTION According to this invention, it is possible to isolate | separate serum, without needing to apply the collected blood to a centrifuge, and can contribute to the reduction of work efficiency and cost in a series of test | inspection work.

That is, the present invention provides a self-collecting blood collection device comprising a container for storing collected blood, a means for collecting serum eluted by spontaneous coagulation of blood, and a container for automatically storing separated and collected serum. Device for collecting and separating blood, a container for storing the collected blood, a means for collecting serum eluted by rapid blood coagulation using a coagulation accelerator, and automatically storing the separated and collected serum A blood collection / separation device by self-blood collection equipped with a container, and 200-
The present invention relates to a serum collection method for collecting 10 to 30 μl of serum with a blood collection volume of 300 μl.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
According to the method of the present invention, it is possible to collect about 10 to 30 μl of serum by collecting a small amount of blood (about 200 to 300 μl), and to obtain 20 to 40 μl with a small blood volume.
Inspection up to the item is possible. In the field of blood testing, in response to attempts to change the method of clinical testing from the past history, there is a strong sense of taboo in Japan while maintaining accuracy, and technological innovation has not progressed. The blood separating apparatus and serum collection method of the present invention follow the current mainstream of biochemical tests using serum, and do not differ from conventional test results at all, maintaining accuracy with a small blood volume. it can.

The blood collection / separation device of the present invention is eluted by a container for storing collected blood and natural coagulation of blood or, if necessary, by rapid blood coagulation using a coagulation accelerator such as silica or thrombin. It is provided with a means for collecting coming serum and a container for automatically containing the separated and collected serum.

[0013] Blood is collected by pressing a separately attached blood collection needle against a finger or the like and dropping the above-mentioned minute amount of blood coming out of the finger or the like into the container. In general, blood collection is defined as a medical practice by law, and must be performed by a certain qualified person such as a doctor, but at your own risk,
The purpose of self-management of health is to perform it by himself, and self-collecting blood performed by a test subject by inserting a blood-collecting needle into his / her finger or the like is an act outside of legal regulations.

Hereinafter, the blood collection and analysis of the present invention will be described with reference to the drawings.
A specific example of the separating device will be described. As a means for collecting serum eluted by blood coagulation from a container containing collected blood, several methods are conceivable, examples of which are shown in FIGS.

FIGS. 1 to 3 show that a means for collecting serum eluted by blood coagulation mounts a serum collection container with a filter for holding a blood clot on a container for storing the collected blood, and then removes the whole container. It is a conceptual diagram by the method of falling over and letting a serum flow naturally into a serum collection container.

In the case of this system, the kit comprises two open containers having caps 6 and 7, respectively, namely the blood container 1
(FIG. 1 (a)) and a serum collection container 2 (FIG. 1 (b)). The serum collection container 2 includes a filter 4 for holding a blood clot.
Is provided.

A method for separating and collecting serum by this method will be described with reference to FIG. First, the blood 8 is dropped into the blood storage container 1 and waits for blood coagulation (FIG. 2 (a)). In this case, it is preferable that the bottom of the blood container 1 is tapered to facilitate the separation of serum, and it is preferable that a coagulation promoter is added as necessary. Also,
Providing the blood guide rod 3 upright from the bottom of the blood container 1 is effective for quick blood collection.

Next, when the blood has coagulated and the serum 9 has been separated (FIG. 2 (b)), the serum collection container 2 with a filter is attached to the blood storage container 1 (FIG. 2 (c)). Here, since the mounting of both containers is required to be hermetically sealed, it is performed by providing appropriate means such as threading 5 at the connecting portion of both containers. FIG. 3 is a diagram showing an example of the inserted cross section of the serum collection container 2.

Next, the whole container after being mounted is turned over, and the serum is allowed to flow naturally into the serum collection container 2,
The serum is separated (FIG. 2 (d)). Blood clot, filter 5
And does not flow into the serum collection container 2 side. Finally, the two containers are detached, and the cap is fitted to the opening of each container to complete the process (FIGS. 2 (e) and 2 (f)).
).

FIGS. 4 and 5 show that a means for collecting serum eluted by blood coagulation is provided by attaching a serum collection container with a capillary to a container containing the collected blood and collecting the serum by capillary action. It is a conceptual diagram by the system (siphon system) which flows into a computer.

In the case of this system, the kit comprises a blood container 10, a siphon 11, a cap 13, and a serum collection container 14.

In order to separate and collect serum by this method, first, blood coagulation is waited in the same manner as described above.
0 (FIG. 5 (c)), a siphon 11 having a size along the opening is attached, and a cap 13 is further covered so that the capillary portion 12 of the siphon 11 penetrates (FIG. 5 (b)). 14 (FIG. 5 (a)) is mounted in an overturned state. At this time, the bottle opening 16 of the serum collection container 14 is plugged with a soft resin such as silicone rubber, and when the capillary portion 12 of the siphon penetrates with some force and the capillary portion is pulled out, Then, the structure is maintained so that the airtightness is maintained again. After the setting is completed, the serum collection container 14 (and the cap 13) is gently lowered (FIG. 4), and the serum flows into the serum collection container 14 by capillary action. When the serum separation is completed, the serum collection container 14 is lifted up and removed from the siphon 11 and turned over again, and the opening of the bottle of the serum collection container 14 is covered to complete the process.

FIG. 6 shows a means for collecting serum eluted by blood coagulation, wherein a container for storing the collected blood and a serum collection container are connected by a serum suction capillary tube, and the serum is collected by pressurization. It is a conceptual diagram by the method of moving to the side.

In the case of this system, the kit comprises a blood storage container 17 and a serum collection container 18, both of which are connected by a serum suction capillary tube 19. In this case, the capillary tube is opened only on the blood storage container 17 side and not opened on the serum collection container 18 side until the serum collection operation after the serum elution (for example, the blood collection container 18 side). The opening is closed and sealed with a soft resin 20 such as silicone rubber). The inside of the serum collection container 18 is kept under reduced pressure.

In order to separate and collect serum by this method, first, blood is dropped into the blood container 17 as described above, and blood coagulation and serum separation are awaited (FIG. 6 (a)). Then
The blood storage container 17 and the serum collection container 18 are operated so as to be close to each other. By this operation, the serum collection container 18 side of the capillary tube 19 breaks through the sealing / sealing portion 20 of the soft resin and opens also to the serum collection container 18 side (FIG. 6B).

With the opening on the serum collection container 18 side, the serum in the blood storage container 17 is drawn through the capillary tube 19 to the serum collection container 18 side, and the serum is collected and separated.
When the serum separation is completed, the blood storage container 17 and the serum collection container 18 are operated so as to be separated from each other. When the blood container is returned to the initial state, the side of the capillary tube 19 on the serum collection container 18 side is closed again, and the process is completed. (FIG. 6 (c)).

In this method, if the blood storage container 17 and the serum collection container 18 are connected only by the serum suction capillary, there may be a problem in terms of strength. Tool may be used. For example,
In addition to the capillary, a spring or the like is bitten, and the blood container 17
The blood collection container 18 and the serum collection container 18 are set with a fixed band so as to keep an appropriate interval between the blood collection container 18 and the serum collection container 18 by hand as described above. It is very convenient to have a structure in which the two containers are separated by the force of the spring when the hands are released after confirming the serum collection after the operation.

[0028]

The blood collection / separation device of the present invention is a very compact and convenient blood collection / separation device for self-collecting blood. Can be separated,
This can contribute to a reduction in work efficiency and cost in a series of inspection work.

[Brief description of the drawings]

FIG. 1 is a schematic view showing an example of a blood collection / separation device according to the present invention, wherein (a) shows a blood storage container and (b) shows a serum collection container 2.

2 (a) to 2 (f) are schematic diagrams illustrating a state in which the blood sampling / separation device of FIG. 1 is used.

FIG. 3 is a schematic view showing an example of an inserted cross section of the serum collection container of FIG. 1 (b).

FIG. 4 is a schematic view showing another example of the blood collection / separation device of the present invention, wherein (a) is a serum collection container, (b) is a siphon, and (c) is a blood container. Shows a container.

5 (a) to 5 (c) are schematic diagrams illustrating a state in which the blood sampling / separating device of FIG. 4 is used.

FIGS. 6 (a) to 6 (c) are schematic views illustrating a state in which still another example of the blood collection / separation device of the present invention is used.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Blood storage container 2 Serum collection container 3 Blood guide rod 4 Filter 5 Screw part 6 Cap 7 Cap 8 Blood 9 Serum 10 Blood storage container 11 Siphon 12 Capillary tube 13 Cap 14 Serum collection container 15 Serum 16 Bottle opening 17 Blood storage container 18 Serum sampling container 19 Serum capillary tube 20 Sealing / sealing part of soft resin

Claims (7)

[Claims] 1. A blood collection by self-collecting blood, comprising a container for storing collected blood, means for collecting serum eluted by spontaneous coagulation of blood, and a container for automatically storing separated and collected serum. -Separation equipment. 2. A container for storing collected blood, means for collecting serum eluted by rapid blood coagulation using a coagulation promoter, and a container for automatically storing separated and collected serum. Blood collection / separation equipment by self-collecting blood. 3. A means for collecting serum eluted by blood coagulation comprises mounting a serum collection container with a filter for holding a blood clot on a container for storing the collected blood, then inverting the entire container, and allowing the container to fall naturally. 3. The blood collection / separation device according to claim 1, wherein the blood serum is flowed into a serum collection container. 4. A method for collecting serum eluted by blood coagulation, wherein a serum collection container with a capillary is mounted on a container containing the collected blood, and the serum flows into the serum collection container by capillary action. The blood collection / separation device according to claim 1 or 2, wherein 5. A means for collecting serum eluted by blood coagulation, wherein a container for storing the collected blood and a serum collection container are connected by a serum suction capillary tube, and the serum is supplied to the serum collection container by pressurization. The blood collection / separation device according to claim 1 or 2, wherein the device is of a moving type. 6. The blood collection / separation device according to claim 1, wherein 10 to 30 μl of serum is collected with a blood collection amount of 200 to 300 μl by self-collecting blood. 7. A blood collection / separation device according to any one of claims 1 to 5, wherein the blood collection / separation device is used to collect 200 to 300 blood samples.
A serum collection method in which 10 to 30 μl of serum is collected with a μl blood collection volume.