JP2001299328A - Powdery composition containing microorganism - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition containing microorganisms and suitable for the production of small-sized bagged product by an automatic three-sided sealing machine while keeping uniform bagged quantity and ensuring good sealing. SOLUTION: A microbial material A is used in combination with a powdery material B having a specific apparent specific gravity and a specific volume- average particle diameter.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism-containing composition, and more particularly to a flushing men's toilet, a flushing unisex toilet, a septic tank, a pumping toilet, a kitchen sink drain, a bathroom sink drain, a restaurant grease strap, and the like. The present invention relates to a powdery microbial material composition effective for deodorization, antifouling and / or cleaning.

[0002]

2. Description of the Related Art Microorganisms such as bacteria, molds and yeasts,
Microbial materials in powder form containing enzymes are known. Microbial material in powder form is easily scattered, so it is easy to contaminate the container when filling it into a suitable container and commercializing it. Particularly when producing bag-shaped products with an automatic three-side seal packaging machine, many defective seals occur I do.

[0003]

The present inventor has proposed a method of improving the scattering property by blending a liquid organic compound with a powdery microbial material (Japanese Patent Application Laid-Open No. 9-206065). Because of the sacrificed fluidity, the three-side seal automatic packaging machine has a content of about 3 to 100 g, especially 30 g.
When the following small products are continuously produced, there is a problem that the filling amount varies.

[0004]

Means for Solving the Problems As a result of intensive studies on the above problems, the present inventors have found that the above objects can be achieved by blending a microbial material and a specific powdery substance, and have reached the present invention. . That is, the present invention provides a powdery microbial material (A) containing at least one type of microorganism selected from the group consisting of bacteria, molds and yeasts.
1 to 70% by mass, and 30 to 99% by mass of at least one kind of powdered substance (B) selected from the group consisting of an alkali metal inorganic salt, an alkaline earth metal inorganic salt, and a mineral product; A powdery microorganism-containing composition having a specific gravity of 0.8 to 1.4 and a volume average particle diameter of 0.04 to 0.4 mm; and a deodorant, an antifouling agent, and / or a deodorant comprising the composition. It is a cleaning agent.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, a microbial material (A) contains at least one kind of microorganisms consisting of bacteria, molds, yeasts, etc., and further contains enzymes, a medium, a carrier and the like, and is usually in powder form. It is. The microorganisms in (A) are in a dormant (inactive) state because they contain substantially no water (about 5% by mass or less). Examples of microorganisms that may be contained in the microorganism material (A) include, for example, genus Bacillus, genus Lactobacillus,
Streptococcus, Streptococcus, Enterococcus, Enterobacter
robacter), Nitrosomonas, Nitrobacter, Cellumon
lomonas), Pseudomonas, Agrobacterium, Rhizopus (Rhi
zopus), Aspergillus, yeast (Ye)
ast) and the like. These microorganisms can be used in combination of two or more. By using them together, they can coexist and cooperate (share roles) in the same environment,
In many cases, it effectively acts on the decomposition of odorous substances.

Among the above microorganisms, Bacillus (Bacill)
us), Lactobacillus, Streptococcus, Enterococcus, and Nitrosomona
s), Nitrobacter, Rhizopus, Aspergillus, and Saccharomyces microorganisms have a large ability to purify sewage by fermenting and decomposing organic matter such as oil in sewage. And Bacillus, Lactobacillus, Streptococcus, Enterococcus
bac), more preferably Bacillus bacteria (A1) and Lactobacillus (Lactobacillus).
s), a combination system with a lactic acid bacterium (A2) consisting of Streptococcus sp.

[0007] In the present invention, examples of the microorganisms include the following. (1) Bacillus (Bacillus) Bacillus subtilis (IFO (Institute for Ferment)
ation Osaka: Fermentation Research Institute, hereinafter the same) 1371
9), Bacillus natto (IFO3013), Bacillus licheniformis (IFO12200), Bacillus coagulans (IFO12583), Bacillus macerans (IFO3490), Bacillus megaterium (IFO1208), Bacillus polymixer (IFO1)
3003). (2) Lactobacillus (Lactobacillus) Lactobacillus casei (IFO12521), Lactobacillus acidophilus (IFO13951), Lactobacillus delbrukki (IFO3202), and the like. (3) Streptococcus genus (Streptococcus) Streptococcus faecalis (IFO3971),
Streptococcus cremoris (IFO3427),
Streptococcus lactis (IFO12007),
Streptococcus salivarius (IFO1395
6), Streptococcus thermophilis (IFO1
3957), Streptococcus faecium (IFO)
12256). (4) Enterococcus Enterococcus faecalis (IFO3971, IF
O3889), Enterococcus feciliam (IFO)
3128, IFO3535) and the like. (5) Enterobacter Enterobacter Aerogenis (IFO12010,
IFO 13534). (6) Genus Nitrosomonas (Nitrosomonas) Nitrosomonas europaea (IFO14298) and the like. (7) Nitrobacter, Nitrobacter agiris (IFO 14297) and the like. (8) Cellulomonas Cellulomonas cellulomonas flavigena (IFO3754), cellulomonas biazotea (IFO12680), cellulomonas seracea (IFO3748), cellulomonas uda (IFO3747), cellulomonas gerida (IFO3)
748). (9) Pseudomonas (Pseudomonas) Pseudomonas putida (IFO14164), Pseudomonas nitroreducens (IFO12694)
Such. (10) Agrobacterium Agrobacterium tumefaciens (IFO30
58, IFO 12667). (11) Rhizopus Rhizopus oryzae (IFO4706), Rhizopus formosaensis (IFO4732), Rhizopus japonicas (IFO4732), Rhizopus delemar (IFO4746) and the like. (12) Aspergillus (Aspergillus) Aspergillus oryzae (IFO30104), Aspergillus niger (IFO31125), Aspergillus usamii (IFO4388), Aspergillus sp.
Sawyer (IFO4391), Aspergillus Terreus (IFO31217) and the like. (13) Saccharomyces Saccharomyces cerevisiae (IFO10217),
Saccharomyces Carlsbergensis (IFO11
67).

In the present invention, the microorganism material (A) can be produced by a conventionally known method, for example, as follows.
That is, appropriate amounts of the above-mentioned microorganisms (at least one), a medium, and a carrier are added to water, mixed, cultured at a temperature of 25 to 50 ° C. for about 24 to 72 hours, and dried by, for example, a spray drying method. And powdered (moisture about 5%). As the culture medium, agricultural and marine products such as fish meal, oily scum, bran, wort and its waste can be used, and as the carrier, inorganic or organic such as calcium carbonate, magnesium carbonate, calcium silicate, diatomaceous earth, zeolite, sawdust and the like. A system powder can be used. The microbial material (A) thus obtained contains proteases usually produced by microorganisms during culturing,
Amylase, lactase, lipase, hemicellulase,
It contains enzymes such as cellulase. In the present invention, in order to further enhance the performance such as the deodorizing property of the microorganism-containing composition, enzymes other than those described above can be added to (A), or the same enzymes can be added later. Total viable cell count in powdered microbial material (A) (unit: pcs / g)
Can be measured using an ordinary medium such as a standard agar medium and appropriately selecting culture conditions (for example, 35 ° C. × 24 to 72 hours) in accordance with a known method such as a mixed plane culture method.

In the present invention, the content of the microorganism culture (excluding the carrier) in the microorganism material (A) is usually 1 to 70% by mass from the viewpoint of deodorizing performance and production cost.
Preferably it is 5 to 60% by mass.

The total viable count of microorganisms contained in 1 g of the microorganism material (A) is usually 10 ⁶ to 10 ¹¹ , preferably 10 ⁷ to 1
Although it is 0 ¹¹ , the total viable count of each microorganism in (A) is preferably in the following range from the viewpoint of deodorizing property and the like. (1) Bacillus ^{(Bacillus): 10 8 ~10 11} / g (2) Lactobacillus ^{(Lactobacillus): 10 6 ~10 10} / g (3) Streptococcus ^{(Streptococcus): 10 6 ~10 10} / g (4) Enterococcus ^{(Enterococcus): 10 6 ~10 9} / g (5) Enterobacter ^{(Enterobacter): 10 6 ~10 9} / g (6) nitrosomonas ^{(nitrosomonas): 10 6 ~10 9} / g (7) Nitrobacter: 10 ⁶ to 10 ⁹ / g (8) Cellulomonas: 10 ⁶ to 10 ⁹ / g (9) Pseudomonas: 10 ⁶ to 10 ⁸ / g (10) Agrobacterium: 10 ⁶ to 10 ⁸ / g (11) Rhizopus: 10 ⁶ to 10 ⁹ / g (12) Aspergillus: 10 ⁶ to 10 ⁹ / g (13) Saccharomyces: 10 ⁶ to 10 ⁸ / g

In the present invention, the powdery substance (B) does not contain microorganisms and has an apparent specific gravity (same as bulk specific gravity; measuring method: J
0.8 to 1.4 and volume average particle diameter (measurement method: according to JIS K0069) 0.04
At least one selected from the group consisting of alkali metal inorganic salts, alkaline earth metal inorganic salts and mineral products having an apparent specific gravity of 0.9 to 1.3 and a volume average particle diameter of 0.06 to 0.3 mm. One kind. Examples of the alkali metal forming the alkali metal inorganic salt (B1) include sodium and potassium, and examples of the inorganic acid forming the (B1) include hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, and carbonic acid. Examples of (B1) include combinations thereof. Specific examples include chlorides such as sodium chloride and potassium chloride, sulfates such as (anhydrous) sodium sulfate and potassium sulfate, nitrates such as sodium nitrate and potassium nitrate, and (anhydrous). ) Phosphates such as sodium phosphate and potassium phosphate, carbonates such as sodium carbonate and potassium carbonate, and the like.
Preferred among these are (anhydrous) sodium sulfate and sodium carbonate. Alkaline earth metal inorganic salt (B
Examples of the alkaline earth metal constituting 2) include magnesium and calcium, and examples of the inorganic acid constituting (B2) include hydrochloric acid, sulfuric acid, phosphoric acid and carbonic acid. Examples of (B2) include combinations thereof. Specific examples include chlorides such as magnesium chloride and calcium chloride, sulfates such as magnesium sulfate and calcium sulfate, phosphates such as magnesium phosphate and calcium phosphate, and magnesium carbonate. And carbonates such as calcium carbonate. Of these, magnesium carbonate and calcium carbonate are preferable mineral products (B3) include kaolin, silica sand, talc and bentonite, and among them, kaolin and talc are preferable. (B1) to (B3) exemplified above can also be used as a mixture. Among the substances (B1) to (B3), (B2),
(B3) is preferred in that it has little effect on the material of the drainage pipe and the like, and calcium carbonate is particularly preferred in that it is inexpensive. The apparent specific gravity of the powdery substance (B) is less than 0.8,
Alternatively, if the volume average particle diameter is less than 0.04 mm, when the composition of the present invention is filled and packaged to produce a bag-shaped product, many defective seal portions tend to occur. If the apparent specific gravity of the powdery substance (B) is more than 1.4 or the volume average particle diameter is more than 0.4 mm, it becomes difficult to mix (A) and (B) uniformly, and the composition of the present invention is filled and packaged. During processing, a variation in the filling amount is likely to occur. As the particle size distribution of (B), the particle size (R1) corresponding to 95% integrated volume and 5%
Ratio (R1 / R) with the particle size (R2) corresponding to the integrated volume
The ratio of R1 / R2 is preferably 2 to 50, and more preferably 5 to 20. In addition, in the above exemplified substances, magnesium carbonate and calcium carbonate used for applications such as plastic fillers,
The volume average particle diameter of general-purpose grade products such as kaolin and talc is 0.005 mm (5 μm) or less, and is 1/10 to 1/1/1 of the volume average particle diameter of the powdery substance (B) of the present invention.
It is 100 or less, and it is necessary to adjust the particle diameter within the range of the present invention by granulation or the like before use. In the present invention, the use ratio of the powdery microorganism material (A) to the powdery substance (B) is usually (A) 1 to 70% by mass, and (B) 30 to 9%.
9% by mass, preferably (A) 2 to 60% by mass, (B) 4
0 to 98% by mass. When the use amount of (A) is less than 1% by mass or the use amount of (B) is more than 99% by mass, it takes an extremely long time for the composition of the present invention to exhibit deodorizing performance, which is not preferable. . When the use amount of (A) is more than 70% by mass or the use amount of (B) is less than 30% by mass, the composition of the present invention is filled and packaged by a three-side seal automatic packaging machine, and is bag-shaped. When a product is manufactured, a defect in a seal portion tends to occur. The outline of the bag making process in the three-side seal automatic packaging machine is shown below. Rolled film (Material: polyethylene,
(Polypropylene, polyethylene terephthalate, polyvinyl alcohol, etc.) are fed out horizontally, then the direction is vertical, the central part is folded into a bag shape, and the lower part is heat-sealed. Next, after heat sealing the side portion, the powder is dropped and filled from the slit of the powder feeder. After heat sealing the open part at the top of the bag, the center of the sealed part is cut horizontally. Continuously and repeatedly, a pouched product is obtained.

In the present invention, a small amount of the organic compound (C) which is liquid at 15 ° C. can be used for the purpose of making the powdery microorganism-containing composition low scattering. (C)
Water-soluble compounds (C1) and water-insoluble compounds (C2). (C1) helps to efficiently disperse the microbial material (A) in water, and (C2) helps to disperse the microbial material (A) in oil floating on the water surface such as wastewater.

Examples of the water-soluble compound (C1) include alkylene glycols having 2 to 6 carbon atoms, such as ethylene glycol, propylene glycol, butylene glycol and hexylene glycol; polyhydric (trivalent to octahydric or higher) alcohols (C3-15, for example, glycerin, diglycerin, polyglycerin); water, monool (C1-7, for example, methanol, ethanol, n
-Butanol, n-hexanol), the above-described alkylene glycol, the above-mentioned polyhydric alcohol, a phenol compound (for example, having 6 to 30 carbon atoms, such as phenol, cresol, butylphenol, nonylphenol, and bisphenol A), a carboxylic acid [a monocarboxylic acid (having a carbon number of 2-7, for example, acetic acid, propionic acid, hexanoic acid), dicarboxylic acid (2-15 carbon atoms, for example, oxalic acid, adipic acid, azelaic acid, itaconic acid, phthalic acid, isophthalic acid, terephthalic acid), trivalent or the like The above polycarboxylic acids (6 to 15 carbon atoms, for example, tricarballylic acid, trimellitic acid, and pyromellitic acid)], [poly (2 to 5) alkylene (2 to 8 carbon atoms) poly (2 to pentavalent or And more) amines (eg, ethylenediamine, diethylenetriamine), ammonia and Alkanolamines (2 carbon atoms
10 to, for example, monoethanolamine, diethanolamine, and triethanolamine), the active hydrogen-containing compound selected from the group consisting of ethylene oxide (hereinafter abbreviated as EO) alone or EO and propylene oxide (hereinafter abbreviated as PO) randomly and / or Or a block addition (EO / PO mass ratio = 20/80 to 95/5) of a water-soluble alkylene oxide adduct having a molecular weight of about 150 to 10,000; a nonionic surfactant having an HLB of 6 to 16 [alkylene oxide (carbon number 2-4) Addition type nonionic surfactants (a product obtained by directly adding an alkylene oxide (2-4 carbon atoms) to a higher alcohol (8-25 carbon atoms), a higher fatty acid (8-25 carbon atoms), etc. Average molecular weight Mn
158 to 200,000); those obtained by reacting a polyalkylene glycol (Mn 150 to 6,000) obtained by adding an alkylene oxide to a glycol with a higher fatty acid or the like; the above-mentioned polyhydric alcohol, pentaerythritol, sorbitol, sucrose, etc. Obtained by reacting a higher fatty acid with a polyhydric alcohol obtained by adding an alkylene oxide to the esterified product (Mn 250 to 30,0)
00 etc.) and polyhydric alcohol type nonionic surfactants (such as the above-mentioned polyhydric alcohols, fatty acid (3 to 25 carbon atoms) esters of polyhydric alcohols such as pentaerythritol, sorbitol and sucrose), etc .; Activator [carboxylic acid (saturated or unsaturated fatty acid having 8 to 25 carbon atoms) or a salt thereof (salts such as sodium, potassium and the above-mentioned alkanolamine), sulfate salt [sulfuric acid ester salt of higher alcohol (8 to 18 carbon atoms) Sulfuric acid ester salt of aliphatic alcohol), higher alkyl ether sulfate salt [sulfuric acid ester salt of EO (1-10 mol) adduct of aliphatic alcohol having 8-25 carbon atoms], sulfonate [alkylbenzene sulfone] Acid salt, alkyl naphthalene sulfonate, dialkyl sulfosuccinate , Alpha-olefin (carbon number 8-2
5) Sulfonate, etc.] and phosphoric ester salts [higher alcohol (8 to 25 carbon atoms) phosphoric acid ester salt, higher alcohol (8 to 25 carbon atoms) EO adduct phosphoric acid ester salt] and the like]; Cationic surfactant [quaternary ammonium salt type [tetraalkyl (C4 to C10)
0) ammonium salts, for example, lauryl trimethyl ammonium chloride, didecyl dimethyl ammonium chloride, stearyl trimethyl ammonium bromide; polyoxyalkylene (2-4 carbon atoms) trialkyl ammonium salts, for example, polyoxyethylene trimethyl ammonium chloride], amine salt type [ Inorganic or organic acid salts of higher aliphatic amines (8 to 25 carbon atoms, for example, laurylamine, stearylamine); inorganic or organic acid salts such as EO adducts of aliphatic amines (1 to 25 carbon atoms) Etc.]; water-soluble amphoteric surfactant [amino acid-type amphoteric surfactant [higher alkylamine (C 8
-25), betaine-type amphoteric surfactants [alkyl (8-25 carbon atoms) dimethyl betaine, etc.], sulfate ester-type amphoteric surfactants [higher alkyl (8-25 carbon atoms) amines] Sulfate sodium salt, hydroxyethylimidazoline sulfate sodium salt, etc.], sulfonate type amphoteric surfactant [pentadecylsulfotaurine, imidazoline sulfonic acid, etc.], phosphate ester type amphoteric surfactant [glycerin higher fatty acid (carbon number 8-25) Phosphate ester amine salts of esterified products, etc.], and mixtures thereof. Of these, alkylene glycols, polyhydric alcohols, water-soluble alkylene oxide adducts, and nonionic surfactants of HLB 6 to 16 are preferable because of their large effect of efficiently dispersing the microbial material (A) in water. Glycols and nonionic surfactants of HLB 6 to 16 are more preferable from the same viewpoint. Here, the water solubility means that the solubility in water (20 ° C.) is 1
This means HLB (Hydrophile-Lipophile Balanc)
e; Griffin method) is an index indicating the balance between hydrophilicity and lipophilicity of a surfactant defined by the following formula. HLB = (molecular weight of hydrophilic group portion of surfactant / molecular weight of surfactant) × 20

As the water-insoluble compound (C2), the above-mentioned active hydrogen-containing compounds, PO alone or EO and P
O is random and / or block added (EO / PO
(Mass ratio = 5 / 95-50 / 50) Mn600-1
HL water-insoluble alkylene oxide adduct; HL
Water-insoluble anionic surfactants; water-insoluble cationic surfactants; water-insoluble amphoteric surfactants and mixtures thereof. Among these, water-insoluble alkylene oxide adducts, HL
Nonionic surfactants having a B6 value or less are preferred from the viewpoint of a large effect of dispersing microorganisms in oil floating on the water surface such as wastewater, and nonionic surfactants having a HLB value of less than 6 are more preferred from the same viewpoint. Here, the term “insoluble in water” means that the solubility in water (20 ° C.) is less than 1% by mass.

In addition to (A) and (B), when using an organic compound (C) which is liquid at 15 ° C., the amount of (C) used is based on the total mass of (A) and (B). From the viewpoint of low scattering property and powder fluidity, the content is preferably 0.1 to 10%, more preferably 0.2 to 5%. Water-soluble compound (C1)
And the water-insoluble compound (C2) can be used in combination, and when used together, the mass ratio is usually C1 / C2 = 1/9 to 9/1,
Preferably it is 3/7 to 8/2.

In the present invention, if necessary, fragrances (natural fragrances, synthetic fragrances and mixtures thereof), coloring agents [inorganic pigments (white pigments such as titanium oxide and zinc white, iron compounds such as iron oxide and navy blue, Sulfides such as cadmium sulfide and ultramarine), organic pigments (such as azo pigments such as azo lake type and monoazo type, and polycyclic pigments such as phthalocyanine type and quinacridone type) and dyes (azo type, triphenylmethane type and diphenylmethane) Phenol-based antioxidants (such as monophenol-based agents such as 2.6-di-t-butyl-p-cresol and butylated hydroxyanisole), and sulfur-based antioxidants (dilauryl 3,3 '-Thiodipropionate and distearyl 3,3'-thiodipropionate Phosphorus antioxidants (such as triphenyl phosphite and triisodecyl phosphite), amine antioxidants (such as octylated diphenylamine and Nn-butyl-p-aminophenol), and ultraviolet absorbers (benzotriazole) UV absorber [2- (2'-hydroxy-5'-methylphenyl)
Benzotriazole and 2- (2'-hydroxy-
5′-t-butylphenyl) benzotriazole, etc.], benzophenone-based ultraviolet absorbers (such as 2-hydroxy-4-methoxybenzophenone and 2,2′-dihydroxy-4-methoxybenzophenone), and salicylic acid-based ultraviolet absorbers (phenylsalicylate) , 4-t-butylphenyl salicylate), acrylate UV absorbers (2-ethylhexyl-2-cyano-3,3'-diphenylacrylate and ethyl-2-cyano-3,
3′-diphenyl acrylate, etc.), chelating agents [hydroxycarboxylic acid and its salts (such as citric acid, sodium citrate, gluconic acid and sodium gluconate), polyamineacetic acid and its salts (ethylenediaminetetraacetic acid, disodium ethylenediaminetetraacetate) salt,
Ethylenediaminetetraacetic acid tetrasodium salt, diethylenetriaminepentaacetic acid and diethylenetriaminepentaacetic acid pentasodium salt), a water-soluble polycarboxylic acid having a weight average molecular weight of 5 to 6,000,000 and salts thereof (polyacrylic acid,
Additives (D) such as polyacrylic acid sodium salt, acrylic acid-maleic acid copolymer and sodium salt of acrylic acid-maleic acid copolymer), and builder (sodium silicate and zeolite) can be used. The amount of these additives is such that the flavor is usually 0.1 to 5%, preferably 0.1 to 2%, based on the total mass of (A) and (B).
%, The colorant is usually 0.01 to 5%, preferably 0.05 to
2%, antioxidant is usually 0.001-2%, preferably 0.1%.
01-0.5%, UV absorber is usually 0.001-2%
Preferably it is 0.01% to 0.5%, and the chelating agent is usually 1 to 20%, preferably 2 to 10%.

When the above components (C) and (D) are further used in addition to the components (A) and (B),
It is preferable to uniformly mix all the components (D). The mixing method is not particularly limited, but the components (A) to (D) may be put into a mixing tank such as a Nauta mixer, and then uniformly stirred and mixed, or first, (A) and (C). May be mixed to make (A) low scattering, and then (B) may be added to (D) if necessary, followed by mixing. Mixing time is usually 1 to 1
It takes about 20 minutes.

According to the above-mentioned method, the composition of the present invention contained in a suitable container, for example, a plastic (polyethylene, polypropylene, polyethylene terephthalate, polyvinyl alcohol, etc.) bag or paper case is taken out of the container and used. Furthermore, in a water-soluble bag or a water-disintegrable bag made of a polyvinyl alcohol (weight average molecular weight Mw 10,000 to 1,000,000) film, paper, or the like, the composition of the present invention is, for example, 3 to 100 g, and more preferably 3 to 30 g. It can be used even if the bag in which the amount is enclosed is directly put into a flush toilet or the like. When the composition of the present invention is placed in a water-soluble or water-disintegrable bag, it is further packaged with a water-insoluble bag (for example, a polybag such as polyethylene, aluminum foil, etc.) so that the bag is not damaged by contact with water during storage. Is preferred.

[0019]

EXAMPLES The present invention will be further described with reference to examples below, but the present invention is not limited to these examples. In the following, parts and% indicate parts by mass and% by mass, respectively. First,
The composition and the evaluation method of the raw materials used in the examples and comparative examples are shown.

1. Microbial material (A) (1) A1: containing about 50% of a carrier (calcium carbonate),
Bacillus subtilis (IFO1371) as a microorganism
9) 8 × 10 ⁹ / g, Bacillus licheniformis (I
FO12200) 1 × 10 ⁹ / g, Enterococcus
E. faecalis (IFO 3971) 6 × 10 ⁸ / g, Rhizopus oryzae (IFO 4706) 3 × 10 ⁷ / g 4
Microbial powder material containing genus / species (2) A2: containing about 50% of a carrier (calcium carbonate),
Bacillus subtilis (IFO 13719) 5 × 10 ⁹
/ G, Bacillus licheniformis (IFO1220
0) 1 × 10 ⁹ / g, Bacillus polymixer (IFO3
020) Microbial powder material containing 3 genera / species of 1 × 10 ⁹ / g

2. Powdery substance (1) B2: calcium carbonate (apparent specific gravity 1.0, volume average particle diameter 0.07 mm) (2) B1: anhydrous sodium sulfate (apparent specific gravity 1.1,
(3) B3: Kaolin (apparent specific gravity 1.2, volume average particle diameter 0.16 mm) (4) b1: Calcium carbonate (apparent specific gravity 1.5, volume average particle diameter 0.8 mm) (5) b2: calcium carbonate (apparent specific gravity 0.57, average particle size 0.005 mm) 3. Organic compound (C) liquid at 15 ° C. (1) C1: propylene glycol (2) C2: nonionic surface activity 3. Agent (polyoxyalkylene glycol of Mn2200 in which EO is added to polypropylene glycol of Mn1700: HLB4.5) Sealability evaluation method Using a three-side seal automatic packaging machine, powder 1 was placed in a polyvinyl alcohol film (width 110 mm, thickness 40 μm).
0.5 g was automatically filled for 1 hour at a bag making speed of 2000 pieces / hour. The pattern of the heat seal portion is a grid pattern, and the seal width is 10 mm. For the total number of the obtained sachets (about 2,000), seal the total number of those having no sealing portion and those having the sealing portion easily peeled off by hand due to contamination of the sealing portion with the filling powder. As the number of defective pieces, a seal defective rate (%) was obtained.

Examples 1 to 4; Comparative Examples 1 to 4 The powdery microorganism-containing composition of the present invention having the composition shown in Table 1 was placed in a polyvinyl alcohol film (thickness: 40 μm) using a three-side seal automatic packaging machine. It enclosed and made 2000 pieces for each prescription. Variations in the filling amount and sealing properties were examined for each formulation, and the results are shown in Table 1. In addition,
The filling amount was set to 10.5 g / piece.

[0023]

[Table 1]

Example 5 950 parts of a microorganism material (A1) and a liquid organic compound (C
1) 50 parts were uniformly mixed with a Nauter mixer to reduce the scattering properties, and then magnesium carbonate (approximately 0.1% specific gravity).
9, volume average particle diameter 0.06 mm) 3000 parts were added and mixed uniformly, and the mixture was sealed in a polyethylene film bag by 15 g by a three-side seal automatic packaging machine. Next, during the grease strap of the cafeteria used by about 400 people a day, 30 g of the contents were broken by breaking the packaging of the two small pouches, and
Every week during the week and twice a week from the next week,
The study continued for three months. As a result, on the grease trap, the unpleasant odor peculiar to sewage was reduced from the 5th day after the start of the test, and the state continued during the test. The water quality inside the grease trap increased in transparency from the second week after the introduction, and the jelly-like solidified oil and other contaminants originally attached to the grease trap wall surface were eliminated. Example 6 950 parts of a microbial material (A2) and a liquid organic compound (C
2) 50 parts were uniformly mixed with a Nauter mixer to reduce scattering, and 3000 parts of kaolin (apparent specific gravity 1.2, volume average particle diameter 0.12 mm) was further added and uniformly mixed. 30 g was sealed in a polyethylene film bag by a three-side seal automatic packaging machine. Next, 30g of contents
Was put into a combined septic tank of a family of four at a time / week, and the test was continued for three months. As a result, the unpleasant odor peculiar to the toilet (the odor in which ammonia, mercaptan and fatty acid were combined) leaked from the septic tank manhole 7 days after the introduction was reduced, and the state continued during the test.

As shown in Table 1, the microorganism-containing compositions (Examples 1 to 4) of the present invention in which the powdered microbial material (A) was used in combination with the specific powdered substance (B) were compared with Comparative Examples (Examples 1 to 4). 1-4)
The dispersion of the filling amount was smaller than that of Comparative Example 1, and there was no defective sealing property. Further, as shown in Examples 5 and 6, the microorganism-containing composition of the present invention is excellent in prevention of soiling by oil, deodorant performance and detergency.

[0026]

EFFECTS OF THE INVENTION The powdery microorganism-containing composition of the present invention not only has excellent performances such as the inherent deodorizing properties of the microorganisms, oil solidification prevention properties, and detergency, but also has an uneven filling amount when packed in small bags. In addition, since there is no poor sealing performance of small bags, processing is extremely easy, and it is most suitable for continuous production. The resulting sachets can be easily and safely handled by housewives who do not have microbial expertise. Therefore,
Promising and practical for purification and deodorization of kitchen drainage such as restaurant grease straps, deodorization of building pits, deodorization of general household septic tanks, pumping toilets, kitchen sinks and bathroom drains, antifouling and cleaning, etc. High value.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) C12N 1/20 C12N 1/20 F // (C12N 1/00 (C12N 1/00 PC12R 1: 125) (C12R 1: 125) (C12N 1/00 (C12N 1/00 P C12R 1:10) C12R 1:10) (C12N 1/00 (C12N 1/00 P C12R 1:12) C12R 1:12) (C12N 1 / 00 (C12N 1/00 P C12R 1:01) C12R 1:01) (C12N 1/00 (C12N 1/00 P C12R 1: 845) C12R 1: 845) (C12N 1/20 (C12N 1/20 D C12R 1: 125) C12R 1: 125) (C12N 1/20 (C12N 1/20 D C12R 1:10) C12R 1:10) (C12N 1/20 (C12N 1/20 DC C12R 1:12) C12R 1: 12) (C12N 1/20 (C 2N 1/20 D C12R 1:01) C12R 1:01) (C12N 1/20 (C12N 1/20 D C12R 1: 845) C12R 1: 845)

Claims (6)

[Claims] 1. A powdery microbial material (A) containing at least one kind of microorganism selected from the group consisting of bacteria, molds and yeasts in an amount of 1 to 70% by mass, and an alkali metal inorganic salt and an alkaline earth metal inorganic material. At least one powdery substance (B) 30 selected from the group consisting of salts and mineral products;
(B) has an apparent specific gravity of 0.8 to
A powdery microorganism-containing composition, having a volume average particle diameter of 1.4 to 0.04 to 0.4 mm. 2. The composition according to claim 1, which is enclosed in a water-soluble or water-disintegrable bag.
A composition as described. 3. The method according to claim 1, wherein (A) contains at least one bacterium belonging to the genus Bacillus, and has a total viable cell count of 10 ⁷ to 10 ^11. / G. 4. The method according to claim 1, wherein (B) is calcium carbonate.
4. The composition according to any one of claims 3 to 3. 5. An organic compound (C) which is liquid at 15 ° C.
The composition according to any one of claims 1 to 4, comprising 0.1 to 10% of the total mass of the composition. 6. A deodorant, an antifouling agent and / or a cleaning agent comprising the composition according to claim 1.