JP2001278312A - Container with joined lid - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a container wherein an error in measurement caused by a contamination can be prevented from occurring by minimizing the contamination. SOLUTION: The container comprises a container body which can receive a liquid and a lid to be fitted into a mouth of the container body, wherein the container body and the lid are joined with each other so that the lid can be opened/closed, and a cut is provided on the lid.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a container for accommodating a liquid, and more particularly, to a container suitably used for measuring a trace substance.

[0002]

2. Description of the Related Art When measuring a specific substance in a sample, it is necessary to avoid contamination from the surroundings. That is, it is necessary to avoid the occurrence of measurement errors in the measurement of the substance to be measured in the sample due to contamination from the surroundings when performing the measurement. The measurement error due to the contamination is caused by a substance to be measured or a substance that interferes with measurement mixed into the sample from the surroundings. The degree of the influence of the contamination on the measured value is inversely proportional to the content of the substance to be measured in the sample.
That is, in the measurement of a trace substance having a low content in a sample, there is a high possibility that contamination occurs,
In particular, care must be taken to avoid contamination. In the case of an element having a small content in the sample and a high Clark number (content in the surface layer of the earth), it can be said that the risk of contamination is even higher.

[0003] In particular, when measuring trace elements in a sample, it is important to prevent contamination from the surroundings. As described above, the degree to which the measured value is affected by the contamination is inversely proportional to the content of the trace element to be measured in the sample.However, the frequency of the influence of the contamination on the measured value depends on the Clark of the trace element. It is proportional to the number. In other words, in the case of trace elements such as gold and platinum, which have a small content in the sample, a low Clark number, and are hardly present in dust and dirt in the air, the risk of contamination is low. .

On the other hand, in the case of trace elements such as aluminum, chromium, nickel, etc., which have a small content in the sample, a high Clark number, and are also present in a large amount in dust and dirt in the air, contamination is difficult. The risk of the nation is high. For this reason, when performing the measurement, it is necessary to handle it with great care so as not to cause contamination (Pathology and Clinical Science, Vol. 17, No. 3, pp. 271-275, 1999). In particular, aluminum is the third most common element after oxygen and silicon in the earth's surface layer, and the first most metallic element. Therefore, in the measurement of this element, the risk of contamination is extremely high. In addition, the living body also contains a trace amount of aluminum at a small but constant concentration. For example, the reference range in serum is 0 to several μg / L (p
pb). In recent years, the relationship between aluminum in serum and various diseases has been clarified,
There is a demand for a simple and highly accurate measurement of aluminum in serum. Conventionally, flameless atomic absorption spectrometry has been used for the measurement of aluminum. However, the measurement of aluminum by the flameless atomic absorption spectrometry is complicated in operation and is affected by the matrix in the serum sample, so that the measurement sensitivity is insufficient for measuring aluminum in the normal range in serum. Problems. Recently, a chromatographic measurement method has been proposed as an alternative measurement method to the flameless atomic absorption spectrometry [Chemical Review No. 9, edited by The Chemical Society of Japan, Societies Publishing Center, pp. 73-79, 1990
Year〕. For example, when measuring aluminum in serum, this measurement method involves a pretreatment of separating and removing proteins using nitric acid or the like, and adding a chelating reagent to a sample from which proteins have been removed to remove aluminum. And a complex is formed and measured by a spectroscopic method.

[0005] In addition, contamination occurs from the time of sampling, and all instruments used for measurement, such as sampling tools, pipettes, measuring containers, and the like, may become sources of contamination. Although contamination of the instrument itself can be avoided by washing the instrument in advance before performing the measurement, it is necessary to consider contamination generated in the process of performing the measurement.

[0006] Particularly, the trace elements such as aluminum described above have a high Clark number and a large content in dust and dirt in the air, so that contamination easily occurs in the course of measurement. This is because when a sample is measured, the sample comes into contact with air contaminated with dust or dust, and trace elements such as aluminum contained in dust or dust in the air are mixed into the sample. Nation occurs.

For example, when aluminum is measured by a chromatographic method or the like, the sample tube must be repeatedly opened and closed in a pretreatment step after the sample is collected in the sample tube and before it is injected into the chromatography. As a result, the frequency at which the sample comes into contact with air contaminated with dust or dust increases.
That is, after centrifuging the protein in the sample, transferring the supernatant of the sample tube to another sample tube, adding a reagent to the sample tube, and further chromatographing the sample or a mixture of the sample and the reagent from the sample tube. It is necessary to open the stopper of the sample tube in the step of transferring the sample tube to the sample tube. As a result, the contamination of the aluminum due to the contamination of the sample by the aluminum contained in the dust and the like in the air causes the measurement error of the aluminum content.

As a method for preventing contamination in the process of performing this measurement, it is conceivable that a container or the like used for measurement is sealed with a lid or the like as shown in FIG. 1A. In this case, the measurement can be performed without opening and closing the lid (while the lid is closed) by performing the following, so that the sample is exposed to air contaminated with dust or dust while keeping the sample substantially sealed. Measurement can be performed without using
Here, when the measurement is performed by a chromatography method or the like using a sealed measurement container, the step of transferring the supernatant of the sample tube to another sample tube after centrifuging the protein in the sample as described above. In the step of adding the reagent to the sample tube, and further in the step of transferring the sample or the mixture of the sample and the reagent from the sample tube to the sample tube for chromatography, the needle is passed through the lid with the measurement container tightly closed. Aspirate or inject the liquid in the sample tube. However, in this case, when the needle penetrates the lid, the needle may hit the lid and bend, or a part of the lid may be scraped and mixed into the container or the needle. In addition, as shown in FIG. 1B, a notch is formed in the lid in advance so that the needle can be easily penetrated, so that the needle is bent as described above, and shavings of the lid are mixed into the container or the needle. A measuring container for preventing the occurrence of the odor has been devised. Further, a container in which a container body and a lid are connected as shown in FIG. 1C has also been devised.

[0009]

However, even if a measuring container such as that shown in FIG. 1B is used, the step of adding a sample or a reagent to the container, or collecting a sample, a reagent or a mixture thereof, etc. It is not easy to minimize the contamination at the stage of performing. This is because trace elements such as aluminum have a high content in topsoil and air, and are easily mixed into the measurement container even by opening and closing the lid when collecting a sample. . That is, when the lid is opened and closed, the hand touches the side (side) or the bottom of the lid, or the lid is placed on a laboratory bench or the like, and the air or surroundings contaminated with dust or dirt is also present. Contamination of aluminum or the like occurs from the above. Further, in the measuring container as shown in FIG. 1C, even if the contamination generated when the lid is opened and closed can be prevented, as described above, in the case of the measurement by the chromatography method, etc. When the needle penetrates through the lid, the needle may hit the lid, causing the needle to bend, or a part of the lid may be scraped and mixed into the container or the needle.

The present invention has been made in view of the above problems, and has as its object to provide a container capable of preventing the occurrence of a measurement error due to contamination by minimizing the contamination. I do.

[0011]

According to the present invention, there is provided a container comprising a container main body capable of storing a liquid and a lid fitted into an opening of the container main body, wherein the container main body and the lid are openable and closable. And the cover has a notch.

Further, in the present invention, it is preferable that the container is used for causing a reaction.

Further, in the present invention, it is preferable that the container is used for measuring a trace substance in a sample.

In the present invention, the container is
It is preferable to use those used for measuring trace elements in a sample.

In the present invention, it is preferable that the container is used for measuring aluminum in a sample.

[0016]

Embodiments of the present invention will be described below with reference to the drawings. An example of the container according to the present invention is shown in FIGS. Reference numeral 1 denotes a main body of the container, and reference numeral 2 denotes a lid, and the main body 1 and the lid 2 are connected to be openable and closable. Further, a notch 3 is formed in the lid 2.

The materials of the container body 1 and the lid 2 include:
Synthetic resin, paper, pulp, glass and the like can be used. As the synthetic resin, for example, urea resin, melamine resin, phenol resin, epoxy resin; polyethylene terephthalate, alkyd resin, unsaturated polyester resin,
Polyester resins such as maleic acid resins; vinyl resins such as polyvinyl chloride, polyvinylidene chloride, polyvinyl alcohol, and vinyl acetal resins; fluororesins such as polyurethane, polytetrafluoroethylene, and polychlorotrifluoroethylene; polyethylene, polypropylene, and polystyrene; Silicone and the like can be mentioned.

The shape of the container body 1 of the present invention may be any shape as long as it can accommodate a liquid inside. When the container body 1 of the present invention is used for measurement with a machine or an apparatus, it may be formed into a shape suitable for the machine or the apparatus.

In the present invention, the container body 1 and the lid 2 are connected so as to be openable and closable. Here, “openably and closably connected” means that the container body 1 and the lid 2 are connected in a state where the lid 2 can be easily opened and closed. That is, as shown in FIG. 2, when the lid 2 is removed from the container main body 1, it is sufficient that the removed lid 2 is connected to the container main body 1 without being separated. The length and width of the connecting portion between the container body 1 and the lid 2 may be appropriately determined according to the size of the container body 1 and the lid 2. Further, the container main body 1 and the lid 2 can be integrally formed, but it is not always necessary to integrally form the container main body 1 and the lid 2, and the container main body 1 and the lid 2 are welded, fused, or pasted in accordance with a material or a manufacturing process. What is necessary is just to shape | mold by etc.

When the container body 1 and the lid 2 are connected in this way, it is not necessary to place the removed lid around each time after the lid is opened. It is possible to prevent the occurrence of contamination due to the attachment of the measurement target substance and the measurement interfering substance. Also,
The lid 2 can be opened and closed with one hand without touching the side (side) or bottom of the lid 2, and the hand is carelessly placed on the side (side) or bottom of the removed lid 2. Contamination caused by touching can also be prevented.

Further, in the present invention, the notch 3 is formed in the lid 2. This cut may be formed in, for example, the upper part of the lid 2 so that the needle or the like can easily penetrate the lid. The notch may be formed at a position where a needle or the like comes down.

Here, when a liquid is stored in the container body 1, the liquid can be stored in the container body 1 by injecting the liquid into the container body 1 and then closing the lid 2. Alternatively, the lid 2 may be fitted to the container body 1 in advance and attached thereto, and then a needle or the like may be passed through the cut 3 formed in the lid body 2 to inject the liquid into the container body 1. Good. Here, when sucking or injecting a liquid such as a sample contained in the container body 1 with a needle or the like, the needle or the like is made to penetrate the notch 3 formed in the lid 2 and the needle or the like is inserted into the container body 1. insert. Thus, a liquid such as a sample contained in the container body 1 can be sucked or injected without removing the lid 2 from the container body 1.

When a liquid such as a sample contained in the container body 1 is measured by a chromatography method or the like, a step of centrifuging a protein in the sample and then transferring a supernatant of the sample tube to another sample tube; When aspirating or injecting a liquid such as a sample with a needle in the step of adding a reagent to a sample tube, or the step of transferring a sample or a mixture of a sample and a reagent from the sample tube to a sample tube for chromatography, etc. The needle is inserted into the container body 1 by passing the needle through the cut 3 formed in the body 2. Thus, a liquid such as a sample contained in the container body 1 can be sucked or injected without removing the lid 2 from the container body 1. In addition, it is possible to automate work when analyzing a liquid such as a sample by a chromatography method or the like.

When the cut 3 is formed in the upper part of the lid 2 as described above, a needle or the like easily penetrates the lid 2 when collecting a sample or the like. That is, when a needle or the like penetrates a part of the cut 3, a rift spreads from that part, so that the needle or the like can be easily inserted into the container body 1, so that the needle or the like may be bent or the cover 2 may be bent. It is possible to prevent shavings from entering the container body 1. In addition, a gap formed in the cut 3 when a needle or the like penetrates the lid 2 can prevent a difference between the outside air pressure and the pressure inside the container body 1 from occurring. For example, when the liquid in the container body 1 is suctioned and collected by a needle or the like, the suction and collection of the liquid causes the inside of the container body 1 to have a negative pressure compared to the outside air, and the suction and collection of the liquid becomes difficult. Can be prevented by a crack generated in the notch 3 when penetrating. The length, width and depth of the notch 3 may be any length as long as a needle or the like can be easily penetrated. Also, the cross-sectional shape of the notch 3 may be any shape as long as it allows easy penetration of a needle or the like, for example, any shape such as a V-shape, a U-shape, a concave shape, and a triangle shape. You may. Further, the shape of the notch 3 on the surface of the lid 2 may be any shape as long as it does not hinder the penetration of a needle or the like.
Any type, such as a mold, may be used. Furthermore, cut 3
Does not have to penetrate the lid 2 as long as it is notched.

The lid connecting container of the present invention as described above can be manufactured by conventionally known injection molding.

In the present invention, a trace substance refers to a substance having a concentration of 0.01% or less in a sample. In the present invention, the term "trace element" refers to a trace element, but a trace element, which is essential to living organisms, and has a biocontent of 0.01%
(100 ppm = 100 μg / g) Refers to the following elements. For example, iron, fluorine, silicon, zinc, rubidium, strontium, lead, manganese, copper, aluminum, cadmium, tin, barium, mercury, selenium, iodine, molybdenum, nickel, chromium, arsenic, cobalt,
Vanadium and the like can be mentioned. Examples of the trace element having a small content in the sample and a high Clark number include, for example, aluminum, chromium, nickel, iron, silicon, and the like.

In the present invention, the term “sample” refers to a liquid which may contain the above-mentioned trace substance or trace element, and is used for confirming the presence of the trace substance or trace element or for quantifying the trace substance in some cases. A thing. For example, human or animal blood, serum, plasma, urine, semen, cerebrospinal fluid, saliva,
Body fluids such as sweat, tears, ascites, and amniotic fluid; extracts of organs such as the brain of humans or animals, hair, skin, nails, muscles, or nerve tissue; extracts or suspensions of human or animal feces; Or a cell extract; a plant extract; food such as cereals, vegetables, fruits, seafood, meat or processed foods, water, tea, coffee,
Examples include drinks such as milk or fruit juice, and environmental analysis samples such as drinking water, river water, lake water, seawater, or soil suspension.

[0028]

EXAMPLE A lid-connected container according to the present invention was manufactured by the following method. In the molding itself, a mold composed of three parts prepared according to the lid connecting container of the present invention is attached to an injection molding machine, high-pressure (30 tons) mold is clamped, and a cavity in the mold is formed. A polyethylene resin melted at 180 ° C. was poured at an injection speed (50%) to form a lid-connected container. Next, the molten polyethylene resin poured into the cavity was cooled by pouring water at 20 ° C. into the mold, solidified, and then taken out of the mold and taken out by a robot. Thus, the lid connecting container of the present invention shown in FIG. 2 was manufactured.

[0029]

According to the present invention, the notch formed in the lid causes the needle to bend when the needle penetrates through the lid and the needle to bend, or a part of the lid to be scraped off inside the container. And mixing in the needle can be prevented. Further, according to the present invention, when collecting a sample containing a trace substance having a very small content in the sample and a high Clark number (content in the surface layer of the earth), for example, by opening and closing the lid, it is used for measurement. It is possible to prevent a trace substance from being mixed into the container. Further, according to the present invention, it is possible to obtain a container capable of preventing the occurrence of a measurement error due to contamination by minimizing the above-mentioned contamination. Further, when the container body and the lid are connected as in the present invention, it is not necessary to place the removed lid around each time after opening the lid, so that the measurement is performed when the lid is placed around the lid. It is possible to prevent the occurrence of contamination due to the attachment of the target substance or the measurement interfering substance. Furthermore, according to the present invention, the lid can be opened and closed with one hand without touching the side (side) or bottom of the lid, so that the side (side) or bottom of the removed lid can be opened and closed. Contamination caused by inadvertently touching the hand can also be prevented. Further, according to the present invention, when a liquid such as a sample contained in the container body is measured by a chromatography method or the like, the liquid such as the sample contained in the container body is sucked without removing the lid from the container body. Or can be injected. In addition, it is possible to automate work when analyzing a liquid such as a sample by a chromatography method or the like. Further, when a notch is formed in the upper portion of the lid as in the present invention, when a needle or the like penetrates a part of the notch, for example, when collecting a sample, a tear is formed from the part and the needle or the like is placed in a container. Since it can be easily inserted into the main body, it is possible to prevent a needle or the like from being bent or shavings of the lid from being mixed into the container body. Furthermore, according to the present invention, the gap in the notch when the needle or the like penetrates can be prevented from generating a difference in pressure between the outside air pressure and the inside of the container body. Therefore, the liquid in the container body is sucked by the needle or the like. At the time of sampling, the inside of the container body becomes negative pressure compared to the outside air due to the suction and sampling of the liquid, and it becomes difficult to suction and sample the liquid.However, this can be prevented by the tears generated by the cut when the needle etc. penetrates it can.

[0030]

[Brief description of the drawings]

FIG. 1 is a view showing a measurement container.

FIG. 2 is a view showing a measurement container.

[Explanation of symbols]

 1: container body, 2: lid, 3: cut

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Aya Okubo 1050 Nippa-cho, Kohoku-ku, Yokohama-shi F-term in Shiseido Basic Research Center, Inc. (Reference) 2G058 BA06 CA02 EA03 EA05 EA08 3E084 AA02 AA12 AA24 AA25 AA26 AA32 AA34 AB01 BA01 CA01 CB01 CB02 CC03 CC04 CC05 CC06 CC07 DA01 DC03 DC04 DC05 DC06 DC07 EA02 EC03 EC04 EC05 EC06 EC08 FA09 GA08 GB12 JA14 JA16 KB01

Claims (5)

[Claims] 1. A container comprising a container main body capable of storing a liquid and a lid fitted to an opening of the container main body, wherein the container main body and the lid are openably connected to each other, and A container having a notch in a lid. 2. The container according to claim 1, wherein the container is used for performing a reaction. 3. The container according to claim 1, wherein the container is used for measuring a trace substance in a sample. 4. The container according to claim 1, wherein the container is used for measuring a trace element in a sample. 5. The container according to claim 1, wherein the container is used for measuring aluminum in a sample.