JP2001081019A - Anticariogenic agent and composition for oral cavity containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an excellent anticariogenic agent capable of inhibiting bacterial plaque formation by Streptococcus mutans by including a yucca extract. SOLUTION: This anticariogenic agent contains a yucca extract. For yucca, its terrestrial stems and other terrestrial parts (including seeds) are usable as materials for extraction; an extraction treatment thereof is carried out by grinding them to an appropriate size and then subjecting the resultant ground product to extraction with water or a hydrophilic organic solvent or their mixture. This anticariogenic agent may be in the form of liquid, solid or semi-solid and can be used in medicaments, quasi-drugs, compositions for oral cavity, and the like. The content of this anticariogenic agent in a composition for oral cavity is pref. 0.01-20 wt.%, more pref. 0.05-10 wt.%, in terms of yucca extract.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an anti-cariogenic agent having an action of inhibiting the formation of dental plaque by cariogenic bacteria, and further relates to an oral composition containing the anti-cariogenic agent.

[0002]

BACKGROUND OF THE INVENTION Dental plaque causing dental caries (cavities) is formed by oral bacteria, mainly Streptococcus mutans. Streptococcus mutans produces glucosyltransferase, a dextran synthase, outside the cells, and synthesizes insoluble glucan, a sticky polysaccharide, using sucrose as a substrate. Oral bacteria proliferate in this insoluble glucan to form plaque. Also,
In oral bacteria such as Streptococcus mutans, acids produced using various sugars demineralize the enamel surface and promote caries. Therefore, preventing the formation of plaque due to Streptococcus mutans is a very important point for preventing dental caries. In recent years, various anti-caries agents have been proposed, but prevention of dental caries is not sufficient, and no effective anti-caries agents that can be actually used have been found yet.

Various methods for preventing the formation of plaque include antibacterial agents for suppressing the growth of Streptococcus mutans, inhibitors of insoluble glucan synthesis by glucosyltransferase, and enzymes for decomposing the formed insoluble glucan. Caries agents are being studied. Among them,
Substances derived from natural products are attracting attention as research targets because of their relatively high safety. For example, Japanese Patent Publication No. 58-12121
No. 8 discloses an anti-carious agent, and specific examples include syrup, fennel, shakuyaku, oren, Japanese gourd, and assemblage. However, the effect is
Far from what is expected, there is currently no effective anti-caries agent that can be used.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to provide an excellent anti-carious agent capable of inhibiting plaque formation by Streptococcus mutans, and furthermore, an oral cavity capable of preventing plaque formation. To provide a composition for use.

[0005]

Means for Solving the Problems The present inventors have conducted intensive studies in order to achieve the above object, and have screened various plant extracts to effectively inhibit plaque formation by Streptococcus mutans. To search for naturally occurring extracts. As a result, they found that the extract of Yucca had a strong inhibitory effect on it and was useful for preventing caries, and completed the present invention. Accordingly, the present invention is an anti-cariogenic agent characterized by containing a Yucca extract. The present invention further provides an oral composition comprising the above anti-carious agent.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
Yucca (Yucca) as raw material includes Yucca arizonica and Yu
cca brevifolia, Yucca schidigera, Yucca elata, Yuc
ca intermdia, Yucca recurvifolia Salisb, Yucca sma
lliana Fernald, Yucca aloifolia Linn, Yucca glorio
sa Linn, Yuccamohavensis, Yucca peninsularis, Yucc
a schottii, Yucca whipplei, and the like. One of these may be used, or two or more thereof may be used. Yucca can be used as an extraction raw material for both above-ground stems and above-ground parts (including seeds). The raw material is appropriately ground to an appropriate size, and then subjected to extraction treatment with water, a hydrophilic organic solvent, or a mixture thereof as an extraction solvent. When a mixture of water and a hydrophilic organic solvent is used, the content of water is suitably up to about 70% by volume of the whole. As the hydrophilic organic solvent for extraction, lower alcohols such as methanol and ethanol, polyhydric alcohols such as glycerin, 1,3-butylene glycol and propylene glycol, and acetone are preferable. The amount of the extraction solvent used is suitably about 3 to 10 times the weight of the extraction raw material.

[0007] The extraction method is not particularly limited, and generally the raw material may be immersed in a solvent maintained at a normal temperature or a temperature near the boiling point. As a method of obtaining a steroid saponin-containing extract without resorting to solvent extraction, there is a method of squeezing a fresh plant body as a raw material. In addition to using an extract obtained by extraction with a predetermined extraction solvent as it is, the extract may be concentrated or dried to a solid (powder or granule). Furthermore, by using a column filled with an ion exchange resin, fractionation is performed using water, a hydrophilic organic solvent or a mixture thereof, and saponin, flavonoid,
An extract containing one or more resveratrol as a main component may be used. As the extract, those commonly used in foods and the like can be used, and they can be obtained on the market. For example, Yucca Formation 50, Yucca Extract Powder, Yucca Sarasaponin 50-M, Yucca Sarasaponin 80-M available from Mitsuba Trading Co., Ltd. are exemplified. In the product of the present invention, such a commercially available product can be used.

The anti-caries agent of the present invention comprises the yucca extract obtained as described above as an active ingredient. The anti-caries agent of the present invention may be any of a liquid, a solid, and a semi-solid. The anti-caries agent of the present invention can be widely used for pharmaceuticals, quasi-drugs, oral compositions, and the like. The content of the anti-cariogenic agent in the oral composition of the present invention is preferably 0.01 to 20% by weight as Yucca extract, which is an active ingredient of the anti-carious agent, based on the total weight of the oral composition. And preferably 0.0
5 to 10% by weight. In the oral composition, if the Yucca extract is less than 0.01% by weight, the effect as an anti-cariogenic agent may be poor, and if it is more than 20% by weight, the effect may plateau and become economically disadvantageous. May be. The oral composition of the present invention is in the form of dentifrice such as toothpaste, lubricating toothpaste, liquid dentifrice, mouthwash, mouthwash, mouthwash, mouthwash tablet, denture cleaner, chewing gum and the like. Can be. These contain the above-mentioned anti-carious agent, but can be prepared by a usual method by blending other components according to the characteristics thereof within a range that does not impair the effects of the present invention.

More specifically, dentifrices such as toothpastes include crystalline silica, amorphous silica, other silica-based abrasives, aluminosilicate, aluminum oxide,
Aluminum hydroxide, insoluble sodium metaphosphate, insoluble potassium metaphosphate, titanium oxide, dibasic calcium phosphate dihydrate, heavy calcium carbonate, light calcium carbonate, etc. (usually 3 to 99% by weight), as a binder Carrageenan, carboxymethylcellulose, methylcellulose, hydroxymethylcellulose, cellulose derivatives such as sodium carboxymethylhydroxymethylcellulose, alkali metal alginate such as sodium alginate, propylene glycol alginate, xanthan gum, tragacanth gum, gum such as gum karaya, gum arabic, polyvinyl alcohol, poly Synthetic binders such as sodium acrylate, carboxyvinyl polymer, polyvinylpyrrolidone, gelling silica, gelling aluminum Rica, Biganto, inorganic binders such as Laponite (usually amount from 0.5 to 10% by weight), sorbitol as thickener, glycerin, ethylene glycol,
Propylene glycol, 1,3-butylene glycol, polyethylene glycol, polypropylene glycol, xylit, maltit, lactit, etc.
50% by weight).

Examples of the surfactant include sodium lauryl sulfate, sodium α-olefin sulfonate, sodium dodecylbenzene sulfonate, sodium lauryl sulfoacetate, sodium N-lauroyl sarcosinate, N-acyl glutamate, and sucrose. Fatty acid esters, polyoxyethylene hydrogenated castor oil, polyoxyethylene polyoxypropylene block copolymers, alkyl glycosides, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, alkyl dimethylamine oxide, etc. 0
~ 5% by weight.

Further, as optional components, for example, sweeteners such as xylitol, sodium saccharin, stevioside, glycyrrhizin, perillartin, thaumatin, asparatylphenylalanine methyl ester, p-methoxycinamic acid, sucrose, fructose, sodium cyclamate, spearmint oil, Peppermint oil, wintergreen oil, sassafras oil, clove oil, eucalyptus oil, sage oil, mayonnaise oil, thyme oil, lemon oil,
Orange oil, 1-menthol, carvone, anethole,
Eugenol, thymol, flavors such as methyl salicylate, preservatives such as benzoic acid, sodium benzoate, p-hydroxypropyl benzoic acid, p-hydroxybutyl benzoic acid, lower fatty acid monoglyceride,

Fungicides such as quaternary ammonium salts, triclosan, and alkyldiaminoethylglycine hydrochloride; epsilon aminocaproic acid; enzymes such as dextranase, amylase, protease, mutanase, lytic enzyme, and lysozyme; sodium monofluorophosphate; Fluorides such as potassium fluorophosphate, sodium fluoride, ammonium fluoride, stannous fluoride, chlorhexidine salts, dihydrocholesterol, glycyrrhetin salts, glycyrrhetinic acid, chlorophyll, caropeptides, vitamins, azulene, lysozyme chloride, anticalculus agents , A plaque inhibitor, potassium nitrate, aluminum lactate and the like. In addition, the addition amount of these optional components can be a normal amount within a range that does not prevent the present invention.

[0013]

The present invention will be described in more detail with reference to the following Test Examples and Examples, which do not limit the scope of the present invention.

[Test Example] <Determination of plaque adhesion> Streptococcus
The effect of suppressing the adhesion of the mutans to the test tube wall was evaluated by the following method. Table 1 shows the results. Streptococcus
Mutant strain 6715 (g type) was cultured at 37 ° C. in a brain heart infusion liquid medium (BHI medium).
0.1 ml of the bacterial solution obtained by culturing for 24 hours, 4.5 ml of 5% sucrose-added BHI medium and 0.5 ml of Yucca extract or sterilized purified water were mixed in a test tube to prepare a test solution. The culture was performed at 37 ° C. for 18 hours. In the above test solution, the concentration of the Yucca extract was varied between 0.2 and 5.0%. After the culture, the BHI medium and the suspended matter were discarded. Next, gently add 5 ml of purified water, and mix with a vortex mixer.
The plaque with weak adhesion was washed by stirring for minutes, and the insoluble matter remaining on the test tube wall was regarded as adherent cells. 5 ml of purified water was added to the test tube, the adherent cells were suspended by sonication, and the turbidity was measured at an absorbance of 550 nm. The plaque adhesion inhibitory activity was evaluated using an experiment using sterile purified water instead of the Yucca extract as a control. The adhesion inhibition rate was calculated by the following equation. Adhesion inhibition rate (%) = (1−turbidity of sample / turbidity of control) × 100

[0014]

[Table 1] Plaque adhesion inhibition rate (%) From Table 1, it was confirmed that the Yucca extract had an inhibitory action on plaque adhesion and was an effective anti-carious agent.

The following is an example of an intraoral composition containing a yucca extract.

Example 1 Preparation of toothpaste A toothpaste was prepared according to a conventional method with the following formulation (unit: wt%). Calcium hydrogen phosphate anhydrate 20.0 Calcium hydrogen phosphate dihydrate 20.0 Silicic anhydride 5.0 Carrageenan 0.7 Sodium carboxymethyl cellulose 0.3 Glycerin 20.0 70% sorbite solution 10.0 Triclosan 0 .1 Saccharin sodium 0.1 Sodium lauryl sulfate 1.5 Yucca extract (Yucca saraponin 80-M) 0.1 Perfume 1.0 Purified water balance Total 100.0%

[0016]

Example 2 Preparation of toothpaste A toothpaste was prepared according to a conventional method with the following formulation (unit: wt%). Silicic anhydride 20.0 Titanium dioxide 0.5 Carrageenan 1.0 Propylene glycol 3.0 70% sorbite solution 20.0 Glycerin 20.0 Sodium monofluorophosphate 0.05 Cetylpyridinium chloride 0.1 Sodium lauryl sulfate 1. 7 Sodium lauroyl sarcosine 0.3 Sodium copper chlorophyllin 0.05 Yucca extract (Yuca sarasaponin 80-M) 0.1 Perfume 1.0 Purified water balance Total 100.0%

[0017]

Example 3 Preparation of toothpaste A toothpaste was prepared according to a conventional method with the following formulation (unit:% by weight). Aluminum hydroxide 30.0 Silicic anhydride 5.0 Sodium chloride 10.0 Sodium carboxymethylcellulose (DS: 0.7) 0.1 Sodium carboxymethylcellulose (DS: 1.0) 0.9 Glycerin 20.0 Sodium lauryl sulfate 1.8 Isopropyl Methylphenol 0.02 dl-α-tocopherol acetate 0.1 Polyoxyethylene hydrogenated castor oil 3.0 Ethyl parahydroxybenzoate 0.1 Yucca extract (Yucca salaponin 80-M) 0.1 Perfume 1.0 Purified water residue 100.0% in total

[0018]

Example 4 Preparation of Toothpaste A toothpaste was prepared according to a conventional method according to the following formulation (unit:% by weight). Calcium pyrophosphate 32.0 Silicic anhydride 6.0 Sodium carboxymethylcellulose (DS: 0.7) 0.2 Sodium carboxymethylcellulose (DS: 1.0) 0.8 Glycerin 25.0 Glycyrrhetinic acid 0.2 Cetylpyridinium chloride 0.05 Epsilon aminocapron Acid 0.1 Yucca extract (Yucca salaponin 80-M) 0.1 Perfume 0.1 Purified water Residue Total 100.0%

[0019]

Example 5 Preparation of Liquid Dentifrice A liquid dentifrice was prepared according to a conventional method with the following formulation (unit:% by weight). Silicic anhydride 10.0 Xanthan gum 1.0 Glycerin 10.0 70% sorbite solution 30.0 Xylitol 1.0 Ethyl parahydroxybenzoate 0.1 Butyl paraoxybenzoate 0.05 Sodium dihydrogen phosphate dihydrate 0. 3 Sodium hydroxide 0.05 Yucca extract (Yucca salsaponin 80-M) 0.1 Perfume 0.5 Purified water balance Total 100.0%

[0020]

Example 6 Preparation of Mouthwash Mouthwash was prepared according to a conventional method with the following formulation (unit: wt%). Denatured Alcohol No. 56 5.0 Polyoxyethylene hydrogenated castor oil 0.5 Glycerin 5.0 Xylitol 25.0 Cetylpyridinium chloride 0.05 Dipotassium glycyrrhizinate 0.05 Ethyl paraoxybenzoate 0.05 Propyl paraoxybenzoate 0.05 Citric acid 0.03 Sodium citrate 0.12 Yellow No. 4 0.0002 Green No. 3 0.0002 Yucca extract (Yucca salasaponin 80-M) 0.1 Purified water Remaining 100.0%

[0021]

Example 7 Preparation of chewing gum Chewing gum was prepared according to a conventional method according to the following formulation (unit:% by weight). Gum base 40.0 Calcium carbonate 2.0 Water candy 15.0 Sugar 30.0 Magnesium citrate 2.55 Sorbic acid 0.2 Yucca extract (Yucca salasaponin 80-M) 0.1 Purified water balance 100.0%

[0022]

Example 8 Preparation of Denture Cleanser Paste A denture cleanser paste was prepared according to a conventional method according to the following formulation (unit:% by weight). Silicic anhydride 3.0 Thickening silica 10.0 Polyethylene glycol 400 5.0 Glycerin 35.0 60% sorbite solution 30.0 Sodium lauryl sulfate 2.0 Sodium carboxymethyl cellulose 1.5 Flavor 1.0 Yucca extract (Yucca) Sarasaponin 80-M) 0.1 Appropriate amount of sodium carbonate Purified water balance Total 100.0%

The anticariogenic agent containing the yucca extract of the present invention as an active ingredient remarkably inhibits plaque formation by Streptococcus mutans. Therefore, by using this as a component of the oral composition, it is possible to provide an oral composition having an excellent effect of preventing dental caries.

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Claims (3)

[Claims] 1. An anti-cariogenic agent comprising a yucca extract. 2. An oral composition comprising the anti-caries agent according to claim 1. 3. An oral composition comprising 0.01 to 20% by weight of a yucca extract.