JP2000050882A - 核移行活性を有するペプチド - Google Patents

Abstract

(57)【要約】 【課題】 核移行活性を有する新規なペプチドおよび該
ペプチドをコードするDNAを提供することを課題とす
る。また、これらを利用した核への物質輸送系を提供す
ることを課題とする。 【解決手段】 転写因子の性質を利用して核移行活性を
有するペプチドをコードするDNAを簡便に単離する方法
を独自に開発し、この方法を利用して核移行活性を有す
るペプチドをコードする複数の新規なDNAを単離するこ
とに成功した。

Description

【発明の詳細な説明】

【０００１】

【発明の属する技術分野】本発明は、細胞内の核へ移行
する活性を有するペプチドおよび該ペプチドをコードす
るDNAに関する。該ペプチドは、核へ物質を輸送するた
めの担体として利用しうるため、本発明は、主として薬
物送達（ドラッグ・デリバリー）の分野に属する。

【０００２】

【従来の技術】薬剤を標的部位に特異的に送達し、標的
部位において至的濃度を維持することは、薬効を効果的
に発現させると共に薬物の副作用を低減させる上で、非
常に重要である。このため特定の標的部位に薬物を送達
するための様々なシステムが開発されてきた。

【０００３】核へ物質を輸送するための方法に関して
は、これまでにマイクロインジェクション法、トランス
フェクション法、抗体法、ペプチド法など多くの方法が
開発されている。

【０００４】マイクロインジェクション法（実験医学別
冊、新遺伝子工学ハンドブック、半土社、173ー176［199
6］）は、微小な注入針を通じて直接的に確実に核内へ
と試料を導入する方法で、通常、核酸やタンパク質など
の高分子物質の導入に頻繁に用いられている。しかし、
核内に注入できる量は微量であり、また、特に試料が核
内で安定に保持されるような性質を持たない場合など
は、注入と同時に試料が核外へと急速に拡散してしまう
ことも考えられる。

【０００５】トランスフェクション法（実験医学別冊、
新遺伝子工学ハンドブック、羊土社、155ー168［199
6］）は、特に外来遺伝子の導入に多用されており、り
ん酸カルシウム法、DEAEデキストラン法、リポソーム
法、リポフェクション法などが知られている。これらの
方法は、試料と担体の間で複合体を形成させた後、貪食
や膜融合により細胞内に取り込ませ，詳細な機構はいま
だ明らかではないが、最終的に試料が核内へ導入される
方法である。

【０００６】抗体法（Avrameas A,et al.Proc.Natl.Aca
d.Sci.USA 1998 May 12；95（10)5601-5606）は、核内
の特別なタンパク質やDNAに対する特異的な抗体を作製
し、抗体自身を担体あるいは担体の一部として使用し
て、試料を核内に導入する方法である。この方法は非常
に優れた方法ではあるが、適切な抗体を得るために時間
と労力が必要となる。

【０００７】ペプチド法（Chaloin L，et al．Biochem
Biophys Res Commun 1998 Feb13;243(2)601-608,Sebest
yen MG，et al. Nat Biotechnol 1998 Jan;16（1）：80
−85）は、抗体法で用いられる抗体の代わりに核移行シ
グナルなどを含んだペプチドを用いる方法で、抗体より
も遥かに小さな分子（最小4〜5アミノ酸残基）で機能す
ることが知られている。さらに、核移行ペプチドは、抗
体などの受動的な拡散による核移行とは異なり、細胞自
身が持つ核移行シグナル依存的なキャリアーを介して能
動的に核内に取り込まれるため、高効率かつ迅速に核移
行が行われるといった優れた特徴を有している。

【０００８】

【発明が解決しようとする課題】本発明は、核移行活性
を有する新規なペプチドおよび該ペプチドをコードする
DNAを提供することを課題とする。また、これらを利用
した核への物質輸送系を提供することを課題とする。

【０００９】

【課題を解決するための手段】本発明者らは、上記課題
を解決するために、まず、転写因子の性質を利用して核
移行活性を有するペプチドをコードするDNAを簡便に単
離する方法を独自に開発した（この方法を「NTT法」と
命名した）。NTT法の原理は、以下の如くである。真核
生物の転写因子は、核内へ移行して特定の遺伝子のプロ
モーター領域と相互作用することにより該特定の遺伝子
の発現を誘導する機能を有しており、転写因子の核移行
能は転写因子中に存在する核移行シグナルに起因してい
ると考えられている。従って、転写因子中の核移行能を
有する領域を除去してその代わりに未知のペプチドが導
入された融合タンパク質を細胞内で発現させた場合、該
融合タンパク質中の未知のペプチドが核移行能を有すれ
ば、融合タンパク質が核内へと移行して特異的なプロモ
ーター領域と作用し、その下流の特定の遺伝子の発現が
誘導される。一方、該融合タンパク質中の未知のペプチ
ドが核移行能を有しなければ、融合タンパク質は核内へ
移行せず、プロモーター領域下流の特定の遺伝子の発現
は誘導されない。このため核移行能を有しない転写因子
に未知のペプチドを融合したタンパク質による、特異的
なプロモーター下流の遺伝子の発現の誘導を指標に、融
合タンパク質中の未知のペプチドが核移行能を有するか
否かの判定を行うことができる。

【００１０】本発明者らは、この原理に基づき、核移行
能を有する領域を除去した転写因子をコードするDNAと
被検DNAとの融合DNAを調製し、転写因子が結合した際に
活性化されるプロモーター領域および該プロモーター領
域の活性化により発現が誘導されるレポーター遺伝子を
核内に保持している真核生物宿主に導入し、該レポータ
ー遺伝子の発現の検出を行った。その結果、被検DNAと
して核移行能を有するペプチドをコードしているDNAを
用いた場合にはレポーター遺伝子の発現が誘導され、一
方、被検DNAとして核移行能を有しないペプチドをコー
ドしているDNAを用いた場合には、レポーター遺伝子の
発現が誘導されなかった。即ち、上記原理に基づき、実
際に、核移行活性を有するペプチドをコードするDNAを
特異的に単離することができることを見出した。

【００１１】そこで、本発明者らは、次に、核移行能を
有する領域を除去した転写因子と他のペプチドとの融合
タンパク質をコードするcDNAのライブラリーを調製し、
これを細胞内に導入してレポーターの発現を指標に核移
行能を有するペプチドをコードするcDNAのスクリーニン
グを行った。その結果、cDNAライブラリーの中から核移
行活性を有するペプチドをコードする複数の新規なDNA
を単離することに成功した。

【００１２】本発明は、本発明者らが独自開発したNTT
法により単離された核移行活性を有するペプチドをコー
ドする新規なDNA、該DNAによりコードされるペプチド、
およびそれらの利用に関する。より具体的には、（１）
配列番号：１から２１、１０１から１０７のいずれか
に記載のアミノ酸配列を含み、核移行活性を有するペプ
チド、（２） 配列番号：１から２１、１０１から１０
７のいずれかに記載のアミノ酸配列において1若しくは
複数のアミノ酸が置換、欠失、および／若しくは付加し
たアミノ酸配列を含み、核移行活性を有するペプチド、
（３） 核へ移行させるための所望のペプチドを含む融
合ペプチドである、（１）または（２）に記載のペプチ
ド、（４） （１）から（３）のいずれかに記載のペプ
チドをコードするDNA、（５） （４）に記載のDNAを含
むベクター、（６） （５）に記載のベクターを保持す
る形質転換細胞、（７） （６）に記載の形質転換細胞
を培養する工程を含む、（１）または（２）に記載のペ
プチドの製造方法、に関する。

【００１３】なお、本発明において「ペプチド」とは、
アミノ酸同士がペプチド結合により結合した化合物を指
す。従って、鎖長の長いペプチドである「ポリペプチ
ド」や「タンパク質」もまた本発明の「ペプチド」に含
まれる。

【００１４】

【発明の実施の形態】本発明は、核移行活性を有する新
規なペプチドに関する。本発明者らが開発したNTT法に
より単離された核移行活性を有するペプチドをコードす
るDNAを配列番号:22から42、および92から100に示す。
また、本発明のペプチドに含まれる、これらDNAにより
コードされるペプチドをそれぞれ配列番号:1から21、お
よび101から107に示す。

【００１５】本発明のペプチドは、核移行活性を有する
という共通の特徴を有する。このため、本発明のペプチ
ドは、核へ輸送することを望む物質を核へ輸送するため
の担体として利用することが可能である。核へ物質を輸
送するために用いる本発明のペプチドとしては、配列番
号:1から21、および101から107のいずれかに記載された
アミノ酸配列を含むペプチドが好適である。

【００１６】但し、核移行活性を有する限り、これらペ
プチドのアミノ酸配列において1若しくは複数のアミノ
酸が置換、欠失、および／若しくは付加したアミノ酸配
列を含むペプチドも同様に用いることが可能である。こ
のようなアミノ酸の改変は、当業者に公知の方法、例え
ば、部位特異的変異誘発法(Curretnt Protocols in Mol
ecular Biology edit. Ausubel et al. (1987) Public
h. Jhon Wily & Sons Section 8.1-8.5) ）やPCR法（PC
R Primer A Laboratory Manual,Cold Spring Harbor L
aboratory Press,1995,581‐621）を利用して行うこと
ができる。アミノ酸の改変部位および改変個数は、改変
後のペプチドが核移行活性を保持する限り特に制限はな
い。アミノ酸の改変個数は、通常は、20アミノ酸以内で
あり、好ましくは10アミノ酸以内であり、さらに好まし
くは5アミノ酸以内であり、さらに好ましくは3アミノ酸
以内である。

【００１７】本発明のペプチドを担体として核へ輸送す
るための物質としては特に制限はない。例えば、ペプチ
ド、核酸、多糖、金属、合成化合物などが挙げられる。
これら物質は、疾患の治療用、予防用、診断用の薬物で
ありうるが、これらに制限されない。臨床応用以外の目
的、例えば、生物学的解析などの目的で用いられる物質
であってもよい。

【００１８】本発明のペプチドを用いて、物質を核へ輸
送するためには、該物質を本発明のベプチドに結合させ
ることが必要である。結合は、一定の結合力が得られる
限り、その形態に制限はない。結合は、架橋剤を用いた
化学的架橋（日本生化学会編続生化学実験講座2、タン
パク質の化学（下）、東京化学同人、604−618）でもよ
く、例えば、従来から頻繁に利用されるジメチルスベロ
イミデート二塩酸塩、スベリン酸ジ-N-ヒドロキシスク
シンイミドエステル、酒石酸ジ-N-ヒドロキシスクシン
イミドエステル、p-フェニレンビスマレイミド、メチル
4−メルカプトブチルイミデート塩酸塩、メチル4−アジ
ドベンゾイミデート塩酸塩、グルタルアルデヒド、N―
スクシンイミジル3-（2-ピリジルジチオ）プロピオネー
ト、N−（γ-マレイミドブチリルオキシ）スクシンイミ
ド、N-（ε-マレイミドヘキサノイルオキシ）スクシン
イミド、1-エチル-3-（3-ジメチルアミノプロピル）カ
ルボジイミドなどが挙げられる。また、核へ輸送させる
ための物質がペプチドであれば、該ペプチドをコードす
るDNAと本発明のペプチドをコードするDNAを連結して、
これらペプチドを融合ペプチドとして発現させることに
より両ペプチドを結合させることも可能である。結合は
スペーサー領域を介した結合であってもよい。一方、結
合形態としては、上述の共有結合的な結合形態以外に、
本発明のペプチドと核へ輸送するための物質の間に生じ
る相互的な親和作用を利用した結合でもよい。このよう
な結合形態として、例えば、静電気的な親和力を利用し
た結合、疎水的な親和力を利用した結合、タンパク質-
タンパク質、あるいは、タンパク質−核酸などの結合モ
チーフを利用した結合、酵素と基質の親和力を利用した
結合などが挙げられる。本発明のペプチドと核へ輸送す
るための物質が本質的にこれらの結合能を有していない
場合は、これらの結合能をもつ機能ドメインを新たに付
加して利用することも考えられる。

【００１９】本発明のペプチドを担体として物質を輸送
するための標的細胞としては、特に制限はなく、本発明
のペプチドを利用する目的に応じて種々の細胞を用いる
ことが可能である。標的細胞としては、例えば、生体に
おける細胞で有り得るし、またインビトロにおける培養
細胞で有り得る。また、これら細胞は、例えば、ヒトを
含む動物細胞、植物細胞、微生物細胞で有り得る。

【００２０】また、本発明は、本発明のペプチドをコー
ドするDNAに関する。本発明のDNAは、本発明のペプチド
をコードしうるものであれば、特に制限はなく、例え
ば、cDNA、ゲノムDNA、および合成DNAが含まれる。本発
明のDNAは、例えば、配列番号:22から42、および92から
100に記載された塩基配列の全部若しくは一部からなるD
NAをプローブとして、ヒト胎児脳由来のcDNAライブラリ
ーまたはゲノムDNAライブラリーをスクリーニングする
ことにより調製することができる。また、配列番号:22
から42、および92から100に記載された塩基配列からな
るDNAに特異的にハイブリダイズするオリゴヌクレオチ
ドをプライマーとして、ヒト胎児脳由来のmRNA、cDNA、
またはゲノムDNAを鋳型としたポリメラーゼ連鎖反応に
より調製することもできる。

【００２１】本発明のDNAは、例えば、本発明のペプチ
ドの製造に利用することが可能である。本発明のペプチ
ドの製造は、一般的には、本発明のDNAを適当なベクタ
ーに挿入して、これを適当な宿主細胞に導入し、得られ
た形質転換細胞を培養して、形質転換細胞に発現させた
組み換えタンパク質を精製することにより行う。組換え
ペプチドの発現には、従来の一般的な発現系を利用する
ことができる。例えば、大腸菌、枯草菌、酵母．昆虫細
胞，植物細胞，動物細胞などを用いた生体内発現系はも
とより、大腸菌、小麦胚芽、あるいはラビットレティキ
ュロサイトなどの細胞抽出液を用いた試験管内発現系も
利用することが可能である。このように発現された組み
換えペプチドは、例えば、従来から用いられているイオ
ン交換、ゲルろ過、分配、アフィニティーなどのクロマ
トグラフィーのステップを組み合わせたり、適当な充填
剤を使用したHPLCなどを利用するなどして高純度に精製
することが可能である。

【００２２】本発明のペプチドは、適当な物質で修飾し
て視覚的に認識できるようすれば、「核輸送指示物質」
として利用することも可能である。すなわち、このよう
に修飾を受けたペプチドを細胞内に導入することによ
り、細胞質から核への物質移動をリアルタイムでモニタ
リングすることが可能となる。ペプチドの修飾方法とし
ては、ペプチドを直接的に蛍光色素などで標識したり、
あるいはEGFPなどの蛍光タンパク質との融合ペプチドを
作製すればよい。このような「核輸送指示物質」は、例
えば、核移行阻害剤などを開発するためのスクリーニン
グ系に応用することができる。

【００２３】

【実施例】以下、本発明を実施例により具体的に説明す
るが、本発明はこれら実施例に制限されるものではな
い。なお、以下の実施例においては、特に記載する場合
を除き、基本的な遺伝子工学的手法は常法（Sambrook,
J., Molecular Cloning: A Laboratory Manual, 1989,
2nd Ed. Cold Spring Harbor Laboratory Press, Cold
Spring Harbor, NY.）に従った。また、制限酵素および
その他の修飾酵素などの遺伝子工学製品は宝酒造から購
入し、それぞれの使用条件は添付マニュアルに従った。
また、大腸菌からのプラスミドの精製は「QIAprep Ki
t」（QIAGEN社製）を用いた。また、塩基配列の確認は
「ABI PRISM 377」（PERKIN ELMER社製）を用いた。解
析用試料の調製は同社の試薬を用い、使用法は製品マニ
ュアルに従った。また、酵母の取り扱い(培地、宿主、
シャトルベクター、遺伝子の導入法、レポーター遺伝子
のアッセイ法、遺伝子の単離法など）については、「MA
TCHMAKER LexA Two-Hybrid System」（CLONTECH社製）
を使用し、添付の「Yeast Protocols Handbook」に従っ
た。また、カスタムオリゴヌクレオチドの合成は東亜合
成に依頼した。

【００２４】［実施例１］ 核移行タンパク質トラップ
ベクターの作製 (1) GAL4転写活性化ドメインをコードするDNA配列のPC
Rによる増幅 5'側にadd-in EcoRIサイトをデザインした「プライマー
NU13」（配列番号:43）および「MATCHMAKER 3' AD LD-I
nsert Screening Amplimer」（配列番号:44）（CLONTEC
H社製）とをプライマーに用い、「プラスミドpACT2」
（CLONTECH社製）を鋳型として、GAL4転写活性化ドメイ
ン（塩基配列を配列番号:45に示す）を含むDNA断片を
「GeneAmp PCR System 2400」（PERKIN ELMER社製）に
て増幅した。Taqポリメラーゼとしては「TaKaRa Ex Ta
q」（TaKaRa社製）を使用し、反応条件などは製品マニ
ュアルに従った。これにより増幅されたDNA断片をエタ
ノール沈殿にて精製し、制限酵素EcoRIとNcoIによる消
化を行った後、6%のポリアクリルアミドゲル電気泳動を
行い、目的のDNA断片をゲルから切り出して電気溶出法
により回収した。

【００２５】(2) LexAタンパク質とGAL4転写活性化ド
メインとの融合タンパク質を発現させるベクター「pLex
AD」の作製 プラスミド「pLexA」（CLONTECH社製）のマルチクロー
ニングサイト内のEcoRIサイトとNcoIサイトの間に上記
(1)のGAL4転写活性化ドメインをコードするDNA断片を挿
入して「pLexAD」を構築した（図１）。塩基配列を決定
して目的の断片が正しく挿入されていることを確認し
た。なお、LexA遺伝子の塩基配列を配列番号:46に示
す。

【００２６】(3) LexAのN末端に核外移行シグナル(NE
S)を挿入したベクター「pLexADrev」の作製 HIVのRevタンパク質が有する核外移行シグナル(配列番
号:47）を以下のように合成し、「pLexAD」がコードす
るLexAタンパク質のN末端近傍のHpaIサイトに挿入し
た。センス鎖として「NU9」（配列番号:48）、アンチセ
ンス鎖として「NU10」（配列番号:49）を合成し、それ
ぞれT4ポリヌクレオチドキナーゼで5'末端をリン酸化し
た後、両者をアニールさせた。このDNA断片を、予めHpa
I消化後アルカリフォスファターゼによる脱リン酸化処
理を行った「pLexAD」に挿入して「pLexADrev」を構築
した（図２）。目的の断片が正しく挿入されていること
は、塩基配列を決定して確認した。

【００２７】(4) LexAタンパク質とGAL4転写活性化ド
メインとの融合タンパク質を発現させるためのCEN/ARS
領域を複製起点にもつプラスミド「pRS1F」とNESを挿入
したLexAタンパク質とGAL4転写活性化ドメインとの融合
タンパク質を発現させるためのCEN/ARS領域を複製起点
にもつプラスミド「pRS3F」の構築 核外移行シグナル(NES)を挿入しない通常のLexAタンパ
ク質とGAL4転写活性化ドメインとの融合タンパク質と、
N末端にNESを挿入したLexAタンパク質とGAL4転写活性化
ドメインとの融合タンパク質（該融合タンパク質のアミ
ノ酸配列を併記した塩基配列を配列番号:50に示す）と
をそれぞれ酵母内で発現するために必要な最小ユニット
は、前者の場合は「pLexAD」を、後者の場合は「pLexAD
rev」をSphIで消化して得られる約1.7kbのDNA断片であ
る。この発現ユニット内には、ADH1プロモーター領域、
発現タンパク質のコード領域、マルチクローニングサイ
ト、ADH1ターミネーター領域が含まれている。それぞれ
の発現ユニットのDNA断片を精製した後、予めプラスミ
ド「pRS413」（STRATAGENE社製）（酵母シャトルベクタ
ー、CEN/ARS origin）のマルチクローニングサイトを含
むPvuII消化断片の部分を、汎用プラスミドpUC19のマル
チクローニングサイトを含むPvuII消化断片で置換たベ
クター「pRSF」のSphIサイトに挿入して「pRS1F」と「p
RS3F」を構築した（それぞれ図３、図４）。目的の断片
が正しく挿入されていることは塩基配列を決定して確認
した。このようにして構築した「pRS1F」(ポジティブコ
ントロール)および「pRS3F」は、転写因子として機能す
る融合タンパク質の直後にpLexA 由来のマルチクローニ
ングサイトが存在しているので、目的のcDNAなどのDNA
断片を常法により容易に融合して発現させることが可能
である。

【００２８】［実施例２］ 人工的な核移行タンパク質
cDNAの融合による核移行タンパク質トラップベクター
「pRS3F」の有効性の実証 (1) 既知のcDNA断片の融合 既知のcDNA断片として、細胞質に局在することが認めら
れている分泌シグナルを除去した緑膿菌の分岐鎖アミノ
酸結合タンパク質('BraC)（該タンパク質のアミノ酸配
列を併記した塩基配列を配列番号:51に示す）（田中真
人、新生化学実験講座６［日本生化学会編］、生体膜と
膜輸送（下）、1992、東京化学同人、9・15）と、そのN
末端にSV40ラージT抗原由来の核移行シグナルを融合さ
せた人工的な核移行タンパク質とをコードするcDNA断片
を「pRS3F」のGAL4転写活性化ドメインのC末端にインフ
レーム（in-frame）で融合させた。実際には、「'Bra
C」をコードするDNA断片(NcoI-DraI)を、予めXhoIで消
化後、クレノウ処理により平滑末端化し、その後NcoI消
化して精製した「pRS3F」に挿入して「pRS3F'BraC」を
構築した。さらに、この「pRS3F'BraC」をNheIとNcoIで
消化して精製したベクターに、SV40ラージT抗原由来の
核移行シグナル（配列番号:52）をコードする合成DNA断
片、すなわち、センス鎖として「NU17」（配列番号:5
3）、アンチセンス鎖として「NU18」（配列番号:54）を
合成し、それぞれT4ポリヌクレオチドキナーゼで5'末端
をリン酸化した後、両者をアニールさせたものを挿入し
て「pRS3FN'BraC」を構築した。また、対照実験のため
に、「'BraC」断片を持たず、核移行シグナルのみを持
つ「pRS3FN」も同様に構築した。目的の断片が正しく挿
入されていることは、塩基配列を決定して確認した。

【００２９】(2) レポーター遺伝子の発現による核移
行能のアッセイ 上述(1)の3種類のプラスミド「pRS3F'BraC」、「pRS3F
N'BraC」、「pRS3FN」と［実施例１］で構築した「pRS1
F」、「pRS3F」をそれぞれ用い、LexAオペレーター配列
を有するプロモーター領域（配列番号:55）（Estojak,
J., Mole. Cell. Biol., 1995, 15: 5820-5829）とその
下流のレポーター遺伝子であるLEU2とβ-ガラクトシダ
ーゼを、それぞれ染色体上とプラスミド上に持つ宿主酵
母EGY48[p8OP-lacZ] (CLONTECH社より入手）を形質転換
した。目的のプラスミドが宿主に導入されたことは、栄
養要求マーカーであるHISの相補性で確認した。次に、
レポーター遺伝子の発現をアッセイするための培地(SD/
-LEU, -HIS, -URA, X-gal)にそれぞれの形質転換体をレ
プリカし、30℃で2-3日培養した。この結果、人工的な
核移行タンパク質を融合させた「pRS3FN'BraC」、核移
行シグナルのみを融合させた「pRS3FN」、またポジティ
ブコントロールとして「pRS1F」を導入したものは、レ
ポーター遺伝子であるβ−ガラクトシダーゼとLEU2の両
方が発現しており、青色の発色と正常な生育の両方が確
認できた（図５、図６）。一方、核移行シグナルを持た
ないタンパク質を融合した「pRS3F'BraC」、何も融合し
ていない「pRS3F」を導入したものは、レポーター遺伝
子はほとんど発現せず、青色の発色も生育も認められな
かった（図５、図６）。

【００３０】以上の結果から、あるペプチドをコードす
るDNA断片を、「pRS3F」がコードする転写因子のC末端
にインフレーム（in-frame）で融合させて、酵母内で発
現させることにより、核移行能の有無をレポーター遺伝
子の発現を見ることで簡便に検出することができた。

【００３１】[実施例３]cDNAライブラリー作製用ベクタ
ーpNSの構築 「pRS3F」の改良を行った。改良点は以下の３点であ
る：LexAとGAL4ADの接合部分のEcoRIサイトを取り除
いた。マルチクローニングサイトに新たにEcoRIサイ
トを導入した。「pRS413」由来の不必要な領域を取り
除き、可能な限り最小化した。

【００３２】まず、「pLexADrev」のEcoRIサイトに合成
リンカー、センス鎖として「NU31」（配列番号:56)、ア
ンチセンス鎖として「NU30」（配列番号:57)を挿入し、
プラスミド「pLexADrev-dE」とした。この「pLexADrev-
dE」を制限酵素SphIで消化した得られた約1.7kbのADH1
発現ユニットを含んだDNA断片を、汎用プラスミドpUC19
のSphIサイトにサブクローニングし、プラスミド「pULe
xADrev-dE」とした。次に、この「pULexADrev-dE」のNh
eIサイトとNcoIサイトの間にEcoRIサイトを持つ合成リ
ンカー、センス鎖として「NU28」（配列番号:58)、アン
チセンス鎖として「NU29」（配列番号:59)を挿入し、プ
ラスミド「pULexADrev-E」とした。一方、「pRS413」を
DraIIIとPvuIIで消化して757bpのマルチクローニングサ
イト含むDNA断片を取り除き、そこにSphIサイトを持つ
合成リンカー、センス鎖として「NU25」（配列番号:6
0)、アンチセンス鎖として「NU26」（配列番号:61)を挿
入し、プラスミド「pRS-S」とした。この「pRS-S」のSp
hIサイトに、前述の「pULexADrev-E」をSphI消化して得
られた約1.7kbのADH1発現ユニットを含んだDNA断片を挿
入して、cDNAライブラリー作製用のベクターpNS（図
７）を構築した（ADH1による転写方向は、HIS３の転写
方向と同一である）。

【００３３】[実施例４] 融合タンパク質発現ライブラ
リー（ヒト培養細胞NT2前駆細胞由来）の作製と核移行
アッセイ (1)融合タンパク質発現ライブラリーの作製 mRNAは、ヒト培養細胞NT2前駆細胞(Stratagene社製)を
添付プロトコール(Catalog #204101, Revision #036002
a)に従って培養し、市販のトータルRNA抽出キットおよ
びmRNA抽出キット(Pharmacia社製)により調製した。そ
の一部(3μg)を使用して市販のcDNA合成キット(Pharmac
ia社製)によりcDNAライブラリーを作製した。具体的に
は、oligo(dT)12-18プライマーを利用してcDNA合成を行
い、pNSベクターのEcoRI／NotIサイトに挿入した。な
お、cDNAの挿入は、Directional Cloning Toolbox (Pha
rmacia社製)を用いることにより、一方向性とした。そ
の後、作製したcDNAライブラリーの一部を用い、常法
(新細胞工学実験プロトコール、秀潤社、114-115)に従
ってエレクトロポレーション(BIO RAD社製 Gene Pulse
r)により市販の大腸菌(GibcoBRL社製 ElectroMAX DH10B
Cells)を形質転換した。得られた形質転換体をアンピ
シリン(100μg/ml)を含むLB寒天培地で30℃で16時間培
養し、集菌後プラスミドを調製した(QIAGEN社製 QIAGEN
Maxi kit)。

【００３４】(2)酵母を用いた核移行アッセイ 調製した融合タンパク質発現ライブラリーのプラスミド
60μgを用いて、常法(CLONTECH社、Yeast Protocols Ha
ndbook, PT3024-1: 17-20)によりEGY48株を形質転換し
た。レポーター遺伝子LEU2の発現によりクローンを選択
する目的でSD寒天培地(-His/-Leu)にて30℃で３〜７日
培養したところ、約1000個のポジティブクローンが得ら
れた。

【００３５】(3)塩基配列の決定 このようにして得られたポジティブクローンの一部(12
クローン)についてベクターに挿入されたcDNA断片の塩
基配列の決定を行った。塩基配列の決定を行うために、
まず各クローンからテンプレートDNAをコロニーPCRによ
って調製した。20μlのPCR反応液（耐熱性DNAポリメラ
ーゼ(TaKaRa社製 Ex Taq) 0.5unit、dNTP mixture 各4n
mol、「プライマーNU15」(配列番号:62)、「プライマー
NU36」(配列番号:63)を各0.4pmol、2μlの添付バッファ
ー、および滅菌水）に各クローンから掻き取った少量の
菌体を加え、「GeneAmp PCR System 2400」(PERKIN ELM
ER社製)で変性94℃、アニーリング60℃、伸張72℃、サ
イクル数40の条件にて挿入cDNA断片の増幅を行った。各
PCR産物について、Microcon-100(Millipore社製)にて脱
塩および未反応プライマーの除去を行いテンプレートDN
Aを得た。こうして得られたテンプレートの一部(100〜2
00ng)を用い、ABI社の製品マニュアルに従った方法で塩
基配列の決定を行った。

【００３６】(4)得られたクローンのデータベース解析 各クローンの塩基配列を公共データベースであるNation
al Center for Biotechnology Information (NCBI) のB
asic BLAST (http://www.ncbi.nlm.nih.gov/cgi-bin/BL
AST/nph-blast?Jform=0)で検索した。その結果、12クロ
ーン全てが既知の遺伝子と一致した。その内、これまで
に核内で機能することが報告あるいは示唆されているの
は10クローンあり、その中の5クローンは塩基性アミノ
酸に富んだSV40ラージT抗原タイプの核移行シグナル様
配列を有するNP220(Inagaki, H.,J. Biol. Chem., 199
6, 271: 12525-12531)、PC4(Ge, H., Cell, 1994, 78:
513-523)、ERC-55(Imai, T., Biochem. Biophys. Res.
Commun., 1997, 233: 765-769)、ヒストン結合タンパク
質(O' Rand, M. G., Dev. Biol., 1992, 154: 37-44)、
プロサイモシンα1(Manrow, R. E., J. Biol. Chem., 1
991, 266: 3916-3924)、1クローンは核内外の往復移動
を司るM9配列を有するhnRNPA1(Michael, W. M., Cell,
1995, 83: 415-422)であった。また、4クローンは既知
の核移行シグナルを有さないferritin H chain(Cai, C.
X., J. Biol. Chem, 1997, 272: 12831-12839)、シャ
ペロニン10(Bonardi, M. A.,Biochem. Biophys. Res. C
ommun., 1995, 206: 260-265)、プロテインキナーゼCイ
ンヒビター-I(Brzoska, P. M., Proc. Natl. Acad. Sc
i., 1995, 92: 7824-7828)、ステロイドレセプターコア
クチベーター1(Onate, S. A., Science, 1995, 270: 13
54-1357)であった。現在のところ核内で機能することが
報告されていない残りの2クローン、トロポミオシン(Li
n, C. -S., Mol. Cell. Biol., 1988, 8: 160-168)、G-
リッチシークエンスファクター-1(Qian, Z., Nucleic A
cids Res., 1994, 22: 2334-2343)については既知の核
移行シグナルは認められなかった。

【００３７】[実施例５] 融合タンパク質発現ライブラ
リー（ヒト胎児脳由来）の作製と核移行アッセイ (1)融合タンパク質発現ライブラリーの作製は、まず、
市販のヒト胎児脳cDNAライブラリー（GibcoBRL社製SUPE
RSCRIPTライブラリー）を添付のプロトコールに従って
増幅した後、QIAGEN社製プラスミド精製キットを用いcD
NA断片をインサートに持つプラスミドを精製した。次
に、その一部（30μg）から２種類の制限酵素EcoRIとNo
tIを用いて切り出されたDNA断片を、0.8%のアガロース
電気泳動により0.7kb〜4kbの長さのcDNAに選別して精製
した。このようにして得られたcDNA断片を前述のpNSベ
クターのEcoRI／NotIサイトに挿入して融合タンパク質
発現ライブラリーを作製し、その一部を用い、常法(新
細胞工学実験プロトコール、秀潤社、114-115)に従って
エレクトロポレーション(BIO RAD社製 Gene Pulser)に
より市販の大腸菌(GibcoBRL社製ElectroMAX DH10B Cell
s)を形質転換した。得られた形質転換体をアンピシリン
(100μg/ml)を含むLB寒天培地で30℃で16時間培養し、
集菌後プラスミドを調製した(QIAGEN社製 QIAGEN Maxi
kit)。

【００３８】(2)酵母を用いた核移行アッセイ 調製した融合タンパク質発現ライブラリーのプラスミド
60μgを用いて、常法(CLONTECH社、Yeast Protocols Ha
ndbook, PT3024-1:17-20)によりEGY48株を形質転換し
た。レポーター遺伝子LEU2の発現によりクローンを選択
する目的でSD寒天培地(-His/-Leu)にて30℃で３〜７日
培養したところ、約1000個のポジティブクローンが得ら
れた。

【００３９】(3)塩基配列の決定 このようにして得られたポジティブクローンの一部(489
クローン)についてベクターに挿入されたcDNA断片の塩
基配列の決定を行った。塩基配列の決定を行うために、
まず各クローンからテンプレートDNAをコロニーPCRによ
って調製した。20μlのPCR反応液（耐熱性DNAポリメラ
ーゼ(TaKaRa社製 Ex Taq) 0.5unit、dNTPmixture各4nmo
l、「プライマーNU15」(配列番号：64)、「プライマーN
U36」配列番号：65)を各0.4pmol、2μlの添付バッファ
ー、および滅菌水）に各クローンから掻き取った少量の
菌体を加え、「GeneAmp PCR System9600」(PERKIN ELME
R社製)で変性94℃、アニーリング60℃、伸張72℃、サイ
クル数40の条件にて挿入cDNA断片の増幅を行った。各PC
R産物について、Microcon-100(Millipore社製)にて脱塩
および未反応プライマーの除去を行いテンプレートDNA
を得た。こうして得られたテンプレートの一部(100〜20
0ng)を用い、ABI社の製品マニュアルに従った方法で部
分的な塩基配列の決定を行った。

【００４０】(4)得られたクローンのデータベース解析 489クローンの塩基配列を公共データベースであるNatio
nal Center for Biotechnology Information (NCBI) の
Basic BLAST (http://www.ncbi.nlm.nih.gov/cgi-bin/B
LAST/nph-blast?Jform=0)で検索した結果、220クローン
が新規の配列あるいは既知のEST（Expressed Sequence
Tag）配列と相同性の高い配列を含む172の遺伝子と一致
した。これらは新規核移行タンパク質の候補遺伝子と考
えられた。

【００４１】(5)新規核移行タンパク質の候補遺伝子の
配列決定 そこで、新規核移行タンパク質の候補である172の遺伝
子のうち、21個に関し、その塩基配列（部分的な配列）
を決定した。塩基配列決定の方法はABI社の製品マニュ
アルに従い、適当なプライマーを設計してプライマーウ
ォーキングを行った。

【００４２】決定した配列に基づき構造上の特徴の解析
も行った。具体的には、連続した6アミノ酸のクラス
ター内に、塩基性のアミノ酸（リジンあるいはアルギニ
ン）が4個以上出現する領域を「推定の核移行シグナル
（NLS）」と定義し、塩基配列から予想されるアミノ酸
配列内にそのようなNLSが存在するか否かを調べた。
アミノ酸配列内に既知の典型的なモチーフ構造（例え
ば、Zinc Finger や Leucine Zipper など）、繰り返し
領域、あるいは、ある種のアミノ酸に富んだ領域の存在
なども調べた。タンパク質の細胞内局在を予測するプ
ログラムである「PSORT」を用いて、核内の局在を予測
した。Coiled-Coil構造を予測するプログラムである
「Coils」（Lupas,A.Methods Enzymol.,1996,266:513-5
25）を用いて、タンパク質内におけるコイル構造を予測
した。その結果を表１および表２に示す。

【００４３】なお、これら表中におけるa)プレーティン
グ効率は、レポーター遺伝子の発現強度の目安として用
いた。DNA100ngを用いてEGY48株の形質転換を行い、レ
ポーター遺伝子（LEU2）の発現を検出するためのSD（-L
EU）培地プレートに直接接種して、出現してくるコロニ
ーの数を示す。このプレーティング効率は核移行能の強
さだけではなく、融合したタンパク質の酵母での転写活
性可能をある程度反映すると考えられる。但し、融合し
たタンパク質が酵母に対して毒性に作用する場合なども
ありうるため、あくまでも目安である。また、b)推定核
局在は、PSORTにおける核局在の予測値N=(%)を示す。

【００４４】

【表１】

【００４５】

【表２】

【００４６】(6)培養細胞（COS-7）における核移行の確
認 上述の21の遺伝子を、EGFP（Enhanced Green Fluoresce
nt Protein）との融合タンパク質として培養細胞（COS-
7）で一過的に発現させ、生細胞内での生産物の局在を
観察することで、得られた個々の遺伝子が実際に核移行
能を有したぺプチドをコードしていることを確認した。
その結果、蓄積の程度に強弱差はあるものの、融合タン
パク質が確かに核内に存在することが認められた（図
８）。

【００４７】[実施例６] NTTクローンの全長取得 NTTクローンの全長を取得するため、実施例５と同様の
方法でクローン hfb030, hfb044, hfb060, hfb066, hfb
101, およびhfb341を単離した。NTTクローンの全長cDNA
の取得は、SuperScript^TM Human Fetal Brain cDNA Lib
rary（Gibco BRL）をソースとしてGENE TRPPER^TM cDNA
Positive Selection System（Gibco BRL）を用いて行っ
た。

【００４８】GENE TRPPERの原理は、一本鎖化したcDNA
Libraryとビオチン化した標的cDNA特異的オリゴヌクレ
オチドをハイブリダイゼーションし、ビオチン-アビジ
ン結合を利用して標的cDNAを分離取得するものである。

【００４９】（１）以下に使用したキットおよび試薬を
示す。 ・SuperScript^TM Human Fetal Brain cDNA Library（Gi
bco BRL） ・GENE TRPPER^TM cDNA Positive Selection System（Gi
bco BRL） ・その付属物： Gene II（f1 intergenic regionのセンス鎖にnickを生
じさせる） Exonuclease III ビオチン-14-dCTP ターミナル・デオキシヌクレオチジル・トランスフェラ
ーゼ ストレプトアビジン-パラマグネティック ビーズ 洗浄バッファー 溶出バッファー dNTPs 修復酵素（Repair enzyme） ・ElectroMAX DH10B^TMCells（Gibco BRL） ・オリゴヌクレオチドA,B,C,D（配列は後述） ・Hybond^TM-N+（Amersham Pharmacia Biotech） ・DIG Oligonucleotide Tailing Kit（BOEHRINGER MANN
HEIM） ・DIG Easy Hyb（BOEHRINGER MANNHEIM） ・DIG Wash and Block Buffer Set（BOEHRINGER MANNHE
IM） ・DIG Nucleic Acid Detection Kit（BOEHRINGER MANNH
EIM） ・KOD Dash（TOYOBO） ・QIAprep Spin Miniprep Kit（QIAGEN） ・BigDye Terminator Cycle Sequencing FS Ready Reac
tion Kit（Perkin-ElmerApplied Biosystems） ・dRhodamine Terminator Cycle Sequencing FS Ready
Reaction Kit（Perkin-Elmer Applied Biosystems） ・ABI PRISM 377XL（Perkin-Elmer Applied Biosystem
s）

【００５０】（２）cDNAライブラリの一本鎖化 全長cDNA取得のソースとしてSuperScript^TM Human Feta
l Brain cDNA Library（Gibco BRL）を使用した。Gene
IIによってライブラリベクターpCMV SPORT2上に存在す
るf1 intergenic regionのセンス鎖にnickを生じさせ、
Exonuclease IIIによって同鎖を消化した。SuperScript
^TM Human Fetal Brain cDNA LibraryはpCMV SPORT2に一
方向にcDNAが挿入されている。f1 intergenic regionと
挿入cDNAのセンス鎖が同一鎖上にあるため、Gene IIお
よびExonuclease III処理によりセンス鎖が消化されア
ンチセンス鎖のみが一本鎖として残る。これによりアン
チセンス鎖のみに一本鎖化したcDNAライブラリを得た。
一本鎖化の確認はアガロース電気泳動によって、未処理
cDNAライブラリおよびGene II処理cDNAライブラリと比
較し行った。

【００５１】（３）オリゴヌクレオチド作製 NTT法によってクローニングされたcDNA断片の塩基配列
に特異的なオリゴヌクレオチドA,B,Cを設計した。NTT法
によってクローニング（5' one pass）されたcDNA断
片、hfb030、hfb044、hfb060、hfb066、hfb101、hfb341
の塩基配列を、順に配列番号：66〜71に示す（表３およ
び４）。各クローンに対して設計したオリゴヌクレオチ
ドA,B,Cは、表３および４（配列番号：72〜89）に示
す。オリゴヌクレオチドAは実線下線で、オリゴヌクレ
オチドBは二重下線で、オリゴヌクレオチドCは点線下線
で表した。但し、オリゴヌクレオチドCはアンチセンス
オリゴヌクレオチドであるため、cDNA配列中ではアニー
リング配列となっている。

【００５２】

【表３】

【００５３】

【表４】

【００５４】オリゴヌクレオチドA,Bはセンス鎖、オリ
ゴヌクレオチドCはアンチセンス鎖とした。厳密性を増
すためオリゴヌクレオチドAはハイブリダイゼーション
用、Bは修復用に別々に設計した。また、オリゴヌクレ
オチドCはコロニーハイブリダイゼーション用およびコ
ロニーPCR用に用いた。また、pCMV SPORT2のcDNA挿入部
位上流に特異的なセンス鎖オリゴヌクレオチドDを設計
した。オリゴヌクレオチドDの配列は配列番号：90に、
オリゴヌクレオチドDに対応するpCMV SPORT2のcDNA挿入
部位上流配列を配列番号：91に示す。オリゴヌクレオチ
ドの合成は東亜合成に依頼した。

【００５５】（４）ハイブリダイゼーション用オリゴヌ
クレオチドのビオチン化 オリゴヌクレオチドAにターミナル・デオキシヌクレオ
チジル・トランスフェラーゼを用いてビオチン-14-dCTP
を3'末端に付加することによりビオチン標識した。ビオ
チン標識の有無はアクリルアミド電気泳動によって確認
した。

【００５６】（５）ハイブリダイゼーションおよび標的
cDNAライブラリの分離 （２）で一本鎖化cDNAライブラリと（４）でビオチン化
したオリゴヌクレオチドAをハイブリダイゼーションし
た。ハイブリダイゼーション溶液にストレプトアビジン
-パラマグネティック ビーズを加え、磁石スタンドに固
定して上清を除くことによってハイブリダイゼーション
していないcDNAを分離した。キットに添付の洗浄バッフ
ァーで数回洗浄した後、溶出バッファーでハイブリダイ
ゼーションした標的cDNAライブラリを溶出した。

【００５７】（６）二本鎖への修復およびクローン化 溶出したcDNAをオリゴヌクレオチドBをプライマーとし
てdNTPsおよび修復酵素を用いて二本鎖cDNAライブラリ
に修復した。修復した二本鎖cDNAライブラリをElectroM
AX DH10BTMCells（Gibco BRL）にエレクトロポレーショ
ンした後、寒天培地上に撒きコロニーを形成させた。標
的cDNAの有無は、（７）コロニーハイブリダイゼーショ
ンあるいは（８）コロニーPCRで判別した。

【００５８】（７）コロニーハイブリダイゼーション 寒天培地上に形成させたコロニーをナイロンメンブレン
Hybond^TM-N+（Amersham Pharmacia Biotech）にトラン
スファーし、DIGラベルしたオリゴヌクレオチドCを用い
てコロニーハイブリダイゼーションを行った。オリゴヌ
クレオチドCのDIGラベルはDIG Oligonucleotide Tailin
g Kit（BOEHRINGER MANNHEIM）を用い、ハイブリダイゼ
ーションはDIG Easy Hyb（BOEHRINGER MANNHEIM）を用
いて行った。検出はDIG Wash and Block Buffer Set（B
OEHRINGER MANNHEIM）およびDIGNucleic Acid Detectio
n Kit（BOEHRINGER MANNHEIM）を用いて行った。

【００５９】（８）コロニーPCR 寒天培地上に形成させたコロニーを鋳型としてKOD Dash
（TOYOBO）を用いてPCRを行った。プライマーとしては
標的cDNAに特異的なオリゴヌクレオチドCおよびクロー
ニングベクターに特異的なオリゴヌクレオチドDを用い
た。PCR反応液をアガロース電気泳動しバンドの有無を
確認した。

【００６０】（９）塩基配列決定 （７）あるいは（８）により目的cDNAを有すると判別し
たコロニーを培養し、プラスミドを抽出して塩基配列を
決定した。プラスミドの抽出はQIAprep Spin Miniprep
Kit（QIAGEN）を用いた。塩基配列の決定はBigDye Term
inator Cycle Sequencing FS Ready Reaction Kit（Per
kin-Elmer Applied Biosystems）あるいはdRhodamine T
erminator Cycle Sequencing FS Ready Reaction Kit
（Perkin-Elmer Applied Biosystems）を用いてABI PRI
SM 377XL（Perkin-Elmer Applied Biosystems）にて行
った。

【００６１】単離された各クローン、hfb030（3092bp、
推定翻訳領域237-1661）、hfb044-1（全長3041bp、推定
翻訳領域48-2735）、hfb044-2（全長3060bp、推定翻訳
領域69-2756）、hfb060-1、hfb060-2、hfb066（全長が
翻訳領域の一部と推定される）、hfb101-1（全長2565b
p、推定翻訳領域474-2120）、hfb101-2（全長2694bp、
推定翻訳領域402-2267）、hfb341（全長6193bp、推定翻
訳領域466-3873）の塩基配列は順に配列番号：92〜100
に示す。また、hfb060-1およびhfb060-2を除くこれらク
ローンがコードするタンパク質の推定アミノ酸配列（HF
B030; 474アミノ酸、HFB044-1; 895アミノ酸、HFB044-
2; 895アミノ酸、HFB066、HFB101-1; 548アミノ酸、HFB
101-2; 621アミノ酸、HFB341; 1135アミノ酸）を、順に
配列番号：101〜107に示す。

【００６２】

【発明の効果】本発明により、核移行活性を有するペプ
チドが提供された。本発明のペプチドは、核へ移行させ
たい所望の物質を核内へ輸送するための担体として有用
である。また、適当な物質で修飾して視覚的に認識でき
るようすれば、核輸送指示物質として利用することも可
能である。

【００６３】

【配列表】 SEQUENCE LISTING <110> Helix Reseach Institute <120> Peptides which possess nuclear-targeting ability <130> H1-805DP2 <140> <141> <150> JP 1998-174065 <151> 1998-06-05 <160> 107 <170> PatentIn Ver. 2.0 <210> 1 <211> 261 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2003 <400> 1 Gly Lys Ser Ser Glu Val Leu Ser Ala Thr Glu Leu Arg Val Gln Leu 1 5 10 15 Ala Gln Lys Glu Gln Glu Leu Ala Arg Ala Lys Glu Ala Leu Gln Ala 20 25 30 Met Lys Ala Asp Arg Lys Arg Leu Lys Gly Glu Lys Thr Asp Leu Val 35 40 45 Ser Gln Met Gln Gln Leu Tyr Ala Thr Leu Glu Ser Arg Glu Glu Gln 50 55 60 Leu Arg Asp Phe Ile Arg Asn Tyr Glu Gln His Arg Lys Glu Ser Glu 65 70 75 80 Asp Ala Val Lys Ala Leu Ala Lys Glu Lys Asp Leu Leu Glu Arg Glu 85 90 95 Lys Trp Glu Leu Arg Arg Gln Ala Lys Glu Ala Thr Asp His Ala Thr 100 105 110 Ala Leu Arg Ser Gln Leu Asp Leu Lys Asp Asn Arg Met Lys Glu Leu 115 120 125 Glu Ala Glu Leu Ala Met Ala Lys Gln Ser Leu Ala Thr Leu Thr Lys 130 135 140 Asp Val Pro Lys Arg His Ser Leu Ala Met Pro Gly Glu Thr Val Leu 145 150 155 160 Asn Gly Asn Gln Glu Trp Val Val Gln Ala Asp Leu Pro Leu Thr Ala 165 170 175 Ala Ile Arg Gln Ser Gln Gln Thr Leu Tyr His Ser His Pro Pro His 180 185 190 Pro Ala Asp Arg Gln Ala Val Arg Val Ser Pro Cys His Ser Arg Gln 195 200 205 Pro Ser Val Ile Ser Asp Ala Ser Ala Ala Glu Gly Asp Arg Ser Ser 210 215 220 Thr Pro Ser Asp Ile Asn Ser Pro Arg His Arg Thr His Ser Leu Trp 225 230 235 240 Arg Gln Ser Arg Pro Ser Ser Glu Glu Pro Ala Gln Pro Tyr Cys Thr 245 250 255 Val Thr Arg Gly Ser 260 <210> 2 <211> 253 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2007 <400> 2 Asp Tyr Glu Glu Ile Thr Ile Asp Pro Thr Cys Ser Trp Lys Pro Val 1 5 10 15 Pro Val Lys Pro Asp Met His Ile Lys Glu Glu Pro Asp Gly Pro Ala 20 25 30 Leu Lys Arg Cys Arg Thr Val Ser Pro Ala His Val Leu Met Pro Ser 35 40 45 Val Met Glu Met Ile Ala Ala Leu Gly Pro Gly Ala Ala Pro Phe Ala 50 55 60 Pro Leu Gln Pro Pro Ser Val Pro Ala Pro Ser Asp Tyr Pro Gly Gln 65 70 75 80 Gly Ser Ser Phe Leu Gly Pro Gly Thr Phe Pro Glu Ser Phe Pro Pro 85 90 95 Thr Thr Pro Ser Thr Pro Thr Leu Ala Glu Phe Thr Pro Gly Pro Pro 100 105 110 Pro Ile Cys Tyr Gln Ser Asp Ile Pro Ser Ser Leu Leu Thr Ser Glu 115 120 125 Lys Ser Thr Val Cys Leu Pro Ser Gln Met Ala Pro Ala Gly His Leu 130 135 140 Asp Pro Thr His Asn Pro Gly Thr Pro Gly Leu His Thr Ser Asn Leu 145 150 155 160 Gly Ala Pro Pro Gly Pro Gln Leu His His Ser Asn Pro Pro Pro Ala 165 170 175 Ser Arg Gln Ser Leu Gly Gln Ala Ser Leu Gly Pro Thr Gly Glu Leu 180 185 190 Ala Phe Ser Pro Ala Thr Gly Val Met Gly Pro Pro Ser Met Ser Gly 195 200 205 Ala Gly Glu Ala Pro Glu Pro Ala Leu Asp Leu Leu Pro Glu Leu Thr 210 215 220 Asn Pro Asp Glu Leu Leu Ser Tyr Leu Gly Pro Pro Asp Leu Pro Thr 225 230 235 240 Asn Asn Asn Asp Asp Leu Leu Ser Leu Phe Glu Asn Asn 245 250 <210> 3 <211> 216 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2017 <400> 3 Lys Ser Glu Ser Asn Glu Asp Leu Phe Gly Leu Val Pro Phe Asp Glu 1 5 10 15 Ile Thr Gly Ser Gln Gln Gln Lys Val Lys Gln Arg Ser Leu Gln Lys 20 25 30 Leu Ser Ser Arg Gln Arg Arg Thr Lys Gln Asp Met Ser Lys Ser Asn 35 40 45 Gly Lys Arg His His Gly Thr Pro Thr Ser Thr Lys Lys Thr Leu Lys 50 55 60 Pro Thr Tyr Arg Thr Pro Glu Arg Ala Arg Arg His Lys Lys Val Gly 65 70 75 80 Arg Arg Asp Ser Gln Ser Ser Asn Glu Phe Leu Thr Ile Ser Asp Ser 85 90 95 Lys Glu Asn Ile Ser Val Ala Leu Thr Asp Gly Lys Asp Arg Gly Asn 100 105 110 Val Leu Gln Pro Glu Glu Ser Leu Leu Asp Pro Phe Gly Ala Lys Pro 115 120 125 Phe His Ser Pro Asp Leu Ser Trp His Pro Pro His Gln Gly Leu Ser 130 135 140 Asp Ile Arg Ala Asp His Asn Thr Val Leu Pro Gly Arg Pro Arg Gln 145 150 155 160 Asn Ser Leu His Gly Ser Phe His Ser Ala Asp Val Leu Lys Met Asp 165 170 175 Asp Phe Gly Ala Val Pro Phe Thr Glu Leu Val Val Gln Ser Ile Thr 180 185 190 Pro His Gln Ser Gln Gln Ser Gln Pro Val Glu Leu Asp Pro Phe Gly 195 200 205 Ala Ala Pro Phe Pro Ser Lys Gln 210 215 <210> 4 <211> 373 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2018 <400> 4 Glu Gln Gln Lys Pro Ala Ser Ser Asp Val Val Leu Pro Ala Thr Met 1 5 10 15 Ser Tyr Thr Gly Phe Val Gln Gly Ser Glu Thr Thr Leu Gln Ser Thr 20 25 30 Tyr Ser Asp Thr Ser Ala Gln Pro Thr Cys Asp Tyr Gly Tyr Gly Thr 35 40 45 Trp Asn Ser Gly Thr Asn Arg Gly Tyr Glu Gly Tyr Gly Tyr Gly Tyr 50 55 60 Gly Tyr Gly Gln Asp Asn Thr Thr Asn Tyr Gly Tyr Gly Met Ala Thr 65 70 75 80 Ser His Ser Trp Glu Met Pro Ser Ser Asp Thr Asn Ala Asn Thr Ser 85 90 95 Ala Ser Gly Ser Ala Ser Ala Asp Ser Val Leu Ser Arg Ile Asn Gln 100 105 110 Arg Leu Asp Met Val Pro His Leu Glu Thr Asp Met Met Gln Gly Gly 115 120 125 Val Tyr Gly Ser Gly Gly Glu Arg Tyr Asp Ser Tyr Glu Ser Cys Asp 130 135 140 Ser Arg Ala Val Leu Ser Glu Arg Asp Leu Tyr Arg Ser Gly Tyr Asp 145 150 155 160 Tyr Ser Glu Leu Asp Pro Glu Met Glu Met Ala Tyr Glu Gly Gln Tyr 165 170 175 Asp Ala Tyr Arg Asp Gln Phe Arg Met Arg Gly Asn Asp Thr Phe Gly 180 185 190 Pro Arg Ala Gln Gly Trp Ala Arg Asp Ala Arg Ser Gly Arg Pro Met 195 200 205 Ala Ser Gly Tyr Gly Arg Met Trp Glu Asp Pro Met Gly Ala Arg Gly 210 215 220 Gln Cys Met Ser Gly Ala Ser Arg Leu Pro Ser Leu Phe Ser Gln Asn 225 230 235 240 Ile Ile Pro Glu Tyr Gly Met Phe Gln Gly Met Arg Gly Gly Gly Ala 245 250 255 Phe Pro Gly Gly Ser Arg Phe Gly Phe Gly Phe Gly Asn Gly Met Lys 260 265 270 Gln Met Arg Arg Thr Trp Lys Thr Trp Thr Thr Ala Asp Phe Arg Thr 275 280 285 Lys Lys Lys Lys Arg Lys Gln Gly Gly Ser Pro Asp Glu Pro Asp Ser 290 295 300 Lys Ala Thr Arg Thr Asp Cys Ser Asp Asn Ser Asp Ser Asp Asn Asp 305 310 315 320 Glu Gly Thr Glu Gly Glu Ala Thr Glu Gly Leu Glu Gly Thr Glu Ala 325 330 335 Val Glu Lys Gly Ser Arg Val Asp Gly Glu Asp Glu Glu Gly Lys Glu 340 345 350 Asp Gly Arg Glu Glu Gly Lys Glu Asp Pro Glu Lys Gly Glu Phe Ser 355 360 365 Trp Gly Ala Trp Cys 370 <210> 5 <211> 189 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2038 <400> 5 Glu Leu Phe Ala Arg Tyr Asp Arg Leu Leu Leu Gln Ser Ser Leu Asp 1 5 10 15 Leu Met Ala Asp Val Val Tyr Cys Pro Arg Pro Cys Cys Gln Leu Pro 20 25 30 Val Met Gln Glu Pro Gly Cys Thr Met Gly Ile Cys Ser Ser Cys Asn 35 40 45 Phe Ala Phe Cys Thr Leu Cys Arg Leu Thr Tyr His Gly Val Ser Pro 50 55 60 Cys Lys Val Thr Ala Glu Lys Leu Met Asp Leu Arg Asn Glu Tyr Leu 65 70 75 80 Gln Ala Asp Glu Ala Asn Lys Arg Leu Leu Asp Gln Arg Tyr Gly Lys 85 90 95 Arg Val Ile Gln Lys Ala Leu Glu Glu Met Glu Ser Lys Glu Trp Leu 100 105 110 Glu Lys Asn Ser Lys Ser Cys Pro Cys Cys Gly Thr Pro Ile Glu Lys 115 120 125 Leu Asp Gly Cys Asn Lys Met Thr Cys Thr Gly Cys Met Gln Tyr Phe 130 135 140 Cys Trp Ile Cys Met Gly Ser Leu Ser Arg Ala Asn Pro Tyr Lys His 145 150 155 160 Phe Asn Asp Pro Gly Ser Pro Cys Phe Asn Arg Leu Phe Tyr Ala Val 165 170 175 Asp Val Asp Asp Asp Ile Trp Glu Asp Glu Val Glu Asp 180 185 <210> 6 <211> 123 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2099 <400> 6 Asp Ala Trp Ala Ser Val Gly Cys Pro Ser Ser Ser Pro Ser Ser Thr 1 5 10 15 Thr Pro Thr Ser His Pro Ser Glu Ala Trp Thr Pro Ala Val Ser Ala 20 25 30 Pro Ala Ser Ala Pro Pro Pro Ala Pro Ala Thr Asp Met Cys His Pro 35 40 45 Pro Ser Glu Ser Trp Ser Arg Ala Cys Lys Gly Gly Arg Pro Gly Pro 50 55 60 Gly Ala Ser Pro Val Thr Glu Pro His Trp Ala Glu Gly Ser Ala Ala 65 70 75 80 Glu Thr Leu Ser Ala Leu Ala Ala Glu Lys Leu Gly Thr Met Ala Gly 85 90 95 Leu Ser Gly Pro Leu Gly Trp His Ser Gly Gly Gln Asp Gly Ala Gly 100 105 110 Gly Asp Leu Ala Pro Asp Leu Glu Pro Tyr Leu 115 120 <210> 7 <211> 125 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2072 <400> 7 Cys Arg Gly Gln Leu Val Gln Val Ser Arg Pro Glu Val Ser Ala Gly 1 5 10 15 Ser Leu Leu Leu Pro Ala Pro Gln Ala Glu Asp His Ser Ser Arg Ile 20 25 30 Leu Tyr Pro Arg Pro Lys Ser Leu Leu Pro Lys Met Met Asn Ala Asp 35 40 45 Met Asp Ala Val Asp Ala Glu Asn Gln Val Glu Leu Glu Glu Lys Thr 50 55 60 Arg Leu Ile Asn Gln Val Leu Glu Leu Gln His Thr Leu Glu Asp Leu 65 70 75 80 Ser Ala Arg Val Asp Ala Val Lys Glu Glu Asn Leu Lys Leu Lys Ser 85 90 95 Glu Asn Gln Val Leu Gly Gln Tyr Ile Glu Asn Leu Met Ser Ala Ser 100 105 110 Ser Val Phe Gln Thr Thr Asp Thr Lys Ser Lys Arg Lys 115 120 125 <210> 8 <211> 376 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2091 <400> 8 Lys Met Asp Val Leu Gln Phe Leu Glu Gly Ile Pro Val Asp Glu Asn 1 5 10 15 Ala Val His Val Leu Val Asp Asn Asn Gly Gln Gly Leu Gly Gln Ala 20 25 30 Leu Val Gln Phe Lys Asn Glu Asp Asp Ala Arg Lys Ser Glu Arg Leu 35 40 45 His Arg Lys Lys Leu Asn Gly Arg Glu Ala Phe Val His Val Val Thr 50 55 60 Leu Glu Asp Met Arg Glu Ile Glu Lys Asn Pro Pro Ala Gln Gly Lys 65 70 75 80 Lys Gly Leu Lys Met Pro Val Pro Gly Asn Pro Ala Val Pro Gly Met 85 90 95 Pro Asn Ala Gly Leu Pro Gly Val Gly Leu Pro Ser Ala Gly Leu Pro 100 105 110 Gly Ala Gly Leu Pro Ser Thr Gly Leu Pro Gly Ser Ala Ile Thr Ser 115 120 125 Ala Gly Leu Pro Gly Ala Gly Met Pro Ser Ala Gly Ile Pro Ser Ala 130 135 140 Gly Gly Glu Glu His Ala Phe Leu Thr Val Gly Ser Lys Glu Ala Asn 145 150 155 160 Asn Gly Pro Pro Phe Asn Phe Pro Gly Asn Phe Gly Gly Ser Asn Ala 165 170 175 Phe Gly Pro Pro Ile Pro Pro Pro Gly Leu Gly Gly Gly Ala Phe Gly 180 185 190 Asp Ala Arg Pro Gly Met Pro Ser Val Gly Asn Ser Gly Leu Pro Gly 195 200 205 Leu Gly Leu Asp Val Pro Gly Phe Gly Gly Gly Pro Asn Asn Leu Ser 210 215 220 Gly Pro Ser Gly Phe Gly Gly Gly Pro Gln Asn Phe Gly Asn Gly Pro 225 230 235 240 Gly Ser Leu Gly Gly Pro Pro Gly Phe Gly Ser Gly Pro Pro Gly Leu 245 250 255 Gly Ser Ala Pro Gly His Leu Gly Gly Pro Pro Ala Phe Gly Pro Gly 260 265 270 Pro Gly Pro Gly Pro Gly Pro Gly Pro Ile His Ile Gly Gly Pro Pro 275 280 285 Gly Phe Ala Ser Ser Ser Gly Lys Pro Gly Pro Thr Val Ile Lys Val 290 295 300 Gln Asn Met Pro Phe Thr Val Ser Ile Asp Glu Ile Leu Asp Phe Phe 305 310 315 320 Tyr Gly Tyr Gln Val Ile Pro Gly Ser Val Cys Leu Lys Tyr Asn Glu 325 330 335 Lys Gly Met Pro Thr Gly Glu Ala Met Val Ala Phe Glu Ser Arg Asp 340 345 350 Glu Ala Thr Ala Ala Val Ile Asp Leu Asn Asp Arg Pro Ile Gly Ser 355 360 365 Arg Lys Val Lys Leu Val Leu Gly 370 375 <210> 9 <211> 105 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2115 <400> 9 Glu Asp Leu Gly Lys Asp Val Glu Phe Glu Val Val Gly Asp Ala Pro 1 5 10 15 Glu Lys Val Gly Pro Lys Gln Ala Glu Asp Ala Ala Lys Ser Ile Thr 20 25 30 Asn Gly Ser Asp Asp Gly Ala Gln Pro Ser Thr Ser Thr Ala Gln Glu 35 40 45 Gln Asp Asp Val Leu Ile Val Asp Ser Asp Glu Glu Asp Ser Ser Asn 50 55 60 Asn Ala Asp Val Ser Glu Glu Glu Arg Ser Arg Lys Arg Lys Leu Asp 65 70 75 80 Glu Lys Glu Asn Leu Ser Ala Lys Arg Ser Arg Ile Glu Gln Lys Glu 85 90 95 Glu Leu Asp Asp Val Ile Ala Leu Asp 100 105 <210> 10 <211> 88 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2206 <400> 10 Pro Gln Leu Val Val Val Gly Glu Glu Gly Phe Pro Asp Thr Gly Ser 1 5 10 15 Asp His Ser Tyr Ser Leu Ser Ser Gly Thr Thr Glu Glu Glu Leu Leu 20 25 30 Arg Lys Leu Asn Glu Gln Arg Asp Ile Leu Ala Leu Met Glu Val Lys 35 40 45 Met Lys Glu Met Lys Gly Ser Ile Arg His Leu Arg Leu Thr Glu Ala 50 55 60 Lys Leu Arg Glu Glu Leu Arg Glu Lys Asp Arg Leu Leu Ala Met Ala 65 70 75 80 Val Ile Arg Lys Lys His Gly Met 85 <210> 11 <211> 303 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2255 <400> 11 Leu Ile Ser Asp Leu Lys Met Glu Arg Glu Ala Arg Glu Asn Gly Ser 1 5 10 15 Lys Ser Pro Ala His Ser Tyr Ser Ser Tyr Asp Ser Gly Lys Asn Glu 20 25 30 Ser Val Asp Arg Gly Ala Glu Asp Leu Ser Leu Asn Arg Gly Asp Glu 35 40 45 Asp Glu Asp Asp His Glu Asp His Asp Asp Ser Glu Lys Val Asn Glu 50 55 60 Thr Asp Gly Val Glu Ala Glu Arg Leu Lys Ala Phe Asn Met Phe Val 65 70 75 80 Arg Leu Phe Val Asp Glu Asn Leu Asp Arg Met Val Pro Ile Ser Lys 85 90 95 Gln Pro Lys Glu Lys Ile Gln Ala Ile Ile Asp Ser Cys Arg Arg Gln 100 105 110 Phe Pro Glu Tyr Gln Glu Arg Ala Arg Lys Arg Ile Arg Thr Tyr Leu 115 120 125 Lys Ser Cys Arg Arg Met Lys Arg Ser Gly Phe Glu Met Ser Arg Pro 130 135 140 Ile Pro Ser His Leu Thr Ser Ala Val Ala Glu Ser Ile Leu Ala Ser 145 150 155 160 Ala Cys Glu Ser Glu Ser Arg Asn Ala Ala Lys Arg Met Arg Leu Glu 165 170 175 Arg Gln Gln Asp Glu Ser Ala Pro Ala Asp Lys Gln Cys Lys Pro Glu 180 185 190 Ala Thr Gln Ala Thr Tyr Ser Thr Ser Ala Val Pro Gly Ser Gln Asp 195 200 205 Val Leu Tyr Ile Asn Gly Asn Gly Thr Tyr Ser Tyr His Ser Tyr Arg 210 215 220 Gly Leu Gly Gly Gly Leu Leu Asn Leu Asn Asp Ala Ser Ser Ser Gly 225 230 235 240 Pro Thr Asp Leu Ser Met Lys Arg Gln Leu Ala Thr Ser Ser Gly Ser 245 250 255 Ser Ser Ser Ser Asn Ser Arg Pro Gln Leu Ser Pro Thr Glu Ile Asn 260 265 270 Ala Val Arg Gln Leu Val Ala Gly Tyr Arg Glu Ser Ala Ala Phe Leu 275 280 285 Leu Arg Ser Ala Asp Glu Leu Glu Asn Leu Ile Leu Gln Gln Asn 290 295 300 <210> 12 <211> 866 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2230 <400> 12 Gly Lys Met Ala Ala Pro Leu Ile Pro Leu Ser Gln Gln Ile Pro Thr 1 5 10 15 Gly Asn Ser Leu Tyr Glu Ser Tyr Tyr Lys Gln Val Asp Pro Ala Tyr 20 25 30 Thr Gly Arg Val Gly Ala Ser Glu Ala Ala Leu Phe Leu Lys Lys Ser 35 40 45 Gly Leu Ser Asp Ile Ile Leu Gly Lys Ile Trp Asp Leu Ala Asp Pro 50 55 60 Glu Gly Lys Gly Phe Leu Asp Lys Gln Gly Phe Tyr Val Ala Leu Arg 65 70 75 80 Leu Val Ala Cys Ala Gln Ser Gly His Glu Val Thr Leu Ser Asn Leu 85 90 95 Asn Leu Ser Met Pro Pro Pro Lys Phe His Asp Thr Ser Ser Pro Leu 100 105 110 Met Val Thr Pro Pro Ser Ala Glu Ala His Trp Ala Val Arg Val Glu 115 120 125 Glu Lys Ala Lys Phe Asp Gly Ile Phe Glu Ser Leu Leu Pro Ile Asn 130 135 140 Gly Leu Leu Ser Gly Asp Lys Val Lys Pro Val Leu Met Asn Ser Lys 145 150 155 160 Leu Pro Leu Asp Val Leu Gly Arg Val Trp Asp Leu Ser Asp Ile Asp 165 170 175 Lys Asp Gly His Leu Asp Arg Asp Glu Phe Ala Val Ala Met His Leu 180 185 190 Val Tyr Arg Ala Leu Glu Lys Glu Pro Val Pro Ser Ala Leu Pro Pro 195 200 205 Ser Leu Ile Pro Pro Ser Lys Arg Lys Lys Thr Val Phe Pro Gly Ala 210 215 220 Val Pro Val Leu Pro Ala Ser Pro Pro Pro Lys Asp Ser Leu Arg Ser 225 230 235 240 Thr Pro Ser His Gly Ser Val Ser Ser Leu Asn Ser Thr Gly Ser Leu 245 250 255 Ser Pro Lys His Ser Leu Lys Gln Thr Gln Pro Thr Val Asn Trp Val 260 265 270 Val Pro Val Ala Asp Lys Met Arg Phe Asp Glu Ile Phe Leu Lys Thr 275 280 285 Asp Leu Asp Leu Asp Gly Tyr Val Ser Gly Gln Glu Val Lys Glu Ile 290 295 300 Phe Met His Ser Gly Leu Thr Gln Asn Leu Leu Ala His Ile Trp Ala 305 310 315 320 Leu Ala Asp Thr Arg Gln Thr Gly Lys Leu Ser Lys Asp Gln Phe Ala 325 330 335 Leu Ala Met Tyr Phe Ile Gln Gln Lys Val Ser Lys Gly Ile Asp Pro 340 345 350 Pro Gln Val Leu Ser Pro Asp Met Val Pro Pro Ser Glu Arg Gly Thr 355 360 365 Pro Gly Pro Asp Ser Ser Gly Ser Leu Gly Ser Gly Glu Phe Thr Gly 370 375 380 Val Lys Glu Leu Asp Asp Ile Ser Gln Glu Ile Ala Gln Leu Gln Arg 385 390 395 400 Glu Lys Tyr Ser Leu Glu Gln Asp Ile Arg Glu Lys Glu Glu Ala Ile 405 410 415 Arg Gln Lys Thr Ser Glu Val Gln Glu Leu Gln Asn Asp Leu Asp Arg 420 425 430 Glu Thr Ser Ser Leu Gln Glu Leu Glu Ala Gln Lys Gln Asp Ala Gln 435 440 445 Asp Arg Leu Asp Glu Met Asp Gln Gln Lys Ala Lys Leu Arg Asp Met 450 455 460 Leu Ser Asp Val Arg Gln Lys Cys Gln Asp Glu Thr Gln Met Ile Ser 465 470 475 480 Ser Leu Lys Thr Gln Ile Gln Ser Gln Glu Ser Asp Leu Lys Ser Gln 485 490 495 Glu Asp Asp Leu Asn Arg Ala Lys Ser Glu Leu Asn Arg Leu Gln Gln 500 505 510 Glu Glu Thr Gln Leu Glu Gln Ser Ile Gln Ala Gly Arg Val Gln Leu 515 520 525 Glu Thr Ile Ile Lys Ser Leu Lys Ser Thr Gln Asp Glu Ile Asn Gln 530 535 540 Ala Arg Ser Lys Leu Ser Gln Leu His Glu Ser Arg Gln Glu Ala His 545 550 555 560 Arg Ser Leu Glu Gln Tyr Asp Gln Val Leu Asp Gly Ala His Gly Ala 565 570 575 Ser Leu Thr Asp Leu Ala Asn Leu Ser Glu Gly Val Ser Leu Ala Glu 580 585 590 Arg Gly Ser Phe Gly Ala Met Asp Asp Pro Phe Lys Asn Lys Ala Leu 595 600 605 Leu Phe Ser Asn Asn Thr Gln Glu Leu His Pro Asp Pro Phe Gln Thr 610 615 620 Glu Asp Pro Phe Lys Ser Asp Pro Phe Lys Gly Ala Asp Pro Phe Lys 625 630 635 640 Gly Asp Pro Phe Gln Asn Asp Pro Phe Ala Glu Gln Gln Thr Thr Ser 645 650 655 Thr Asp Pro Phe Gly Gly Asp Pro Phe Lys Glu Ser Asp Pro Phe Arg 660 665 670 Gly Ser Ala Thr Asp Asp Phe Phe Lys Lys Gln Thr Lys Asn Asp Pro 675 680 685 Phe Thr Ser Asp Pro Phe Thr Lys Asn Pro Ser Leu Pro Ser Lys Leu 690 695 700 Asp Pro Phe Glu Ser Ser Asp Pro Phe Ser Ser Ser Ser Val Ser Ser 705 710 715 720 Lys Gly Ser Asp Pro Phe Gly Thr Leu Asp Pro Phe Gly Ser Gly Ser 725 730 735 Phe Asn Ser Ala Glu Gly Phe Ala Asp Phe Ser Gln Met Ser Lys Pro 740 745 750 Pro Pro Ser Gly Pro Phe Thr Ser Ser Leu Gly Gly Ala Gly Phe Ser 755 760 765 Asp Asp Pro Phe Lys Ser Lys Gln Asp Thr Pro Ala Leu Pro Pro Lys 770 775 780 Lys Pro Ala Pro Pro Arg Pro Lys Pro Pro Ser Gly Lys Ser Thr Pro 785 790 795 800 Val Ser Gln Leu Gly Ser Ala Asp Phe Pro Glu Ala Pro Asp Pro Phe 805 810 815 Gln Pro Leu Gly Ala Asp Ser Gly Asp Pro Phe Gln Ser Lys Lys Gly 820 825 830 Phe Gly Asp Pro Phe Ser Gly Lys Asp Pro Phe Val Pro Ser Ser Ala 835 840 845 Ala Lys Pro Ser Lys Ala Ser Ala Ser Gly Phe Ala Asp Phe Thr Ser 850 855 860 Val Ser 865 <210> 13 <211> 269 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2063 <400> 13 Asp Ala Trp Ala Val Lys Glu Glu Cys Arg Leu Leu Asn Ala Pro Pro 1 5 10 15 Val Pro Pro Arg Gly Gly Asn Gly Ser Gly Arg Leu Ser Ser Ser Pro 20 25 30 Pro Val Pro Pro Arg Phe Pro Lys Leu Gln Pro Val His Ser Pro Ser 35 40 45 Ser Ser Leu Ser Tyr Tyr Ser Ser Gly Leu Gln Asp Gly Ala Gly Ser 50 55 60 Arg Ser Gly Ser Gly Ser Pro Ser Pro Asp Thr Tyr Ser Leu Tyr Cys 65 70 75 80 Tyr Pro Cys Thr Trp Gly Asp Cys Lys Glu Pro Val Leu Glu Pro Phe 85 90 95 Asp Pro Phe Glu Leu Gly Gln Gly Ser Ser Pro Glu Pro Glu Leu Leu 100 105 110 Arg Ser Gln Glu Pro Arg Ala Val Gly Thr Pro Gly Pro Gly Pro Arg 115 120 125 Leu Ser Pro Leu Gly Pro Ser Lys Ala Phe Glu Pro Glu Gly Leu Val 130 135 140 Leu His Gln Val Pro Thr Pro Leu Ser Pro Ala Ala Leu Gln Gly Pro 145 150 155 160 Glu Ala Gly Gly Ala Leu Phe Leu Thr Gln Gly Arg Leu Glu Gly Pro 165 170 175 Pro Ala Ser Pro Arg Asp Gly Ala Thr Gly Phe Gly Val Arg Asp Ala 180 185 190 Ser Ser Trp Gln Pro Pro Ala Asp Leu Ser Ala Leu Ser Leu Glu Glu 195 200 205 Val Ser Arg Ser Leu Arg Phe Ile Gly Leu Ser Glu Asp Val Val Ser 210 215 220 Phe Phe Ala Arg Glu Arg Ile Asp Gly Ser Ile Phe Val Gln Leu Ser 225 230 235 240 Glu Asp Ile Leu Ala Asp Asp Phe His Leu Thr Lys Leu Gln Val Lys 245 250 255 Lys Ile Met Gln Phe Ile Lys Gly Trp Arg Pro Lys Ile 260 265 <210> 14 <211> 54 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2411 <400> 14 Glu Glu Glu Glu Glu Glu Ser Glu Asn Lys Ser Met Asp Thr Asp Pro 1 5 10 15 Glu Thr Ile Arg Asp Glu Glu Glu Thr Gly Asp His Ser Met Asp Asp 20 25 30 Ser Ser Glu Asp Gly Lys Met Glu Thr Lys Ser Asp His Glu Glu Asp 35 40 45 Asn Met Glu Asp Gly Met 50 <210> 15 <211> 224 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2436 <400> 15 Ser Ser Asp Pro Ala Asp Phe Glu Pro Ile Pro Ser Phe Ser Gly Phe 1 5 10 15 Pro Leu Asp Ser Pro Lys Thr Leu Val Leu Asp Phe Glu Thr Glu Gly 20 25 30 Glu Arg Asn Ser Pro Asn Pro Arg Ser Val Arg Ile Pro Ser Pro Asn 35 40 45 Ile Leu Lys Thr Gly Leu Thr Glu Asn Val Asp Arg Gly Leu Gly Gly 50 55 60 Leu Glu Gly Thr His Gln Ala Leu Asp Leu Leu Ala Gly Gly Met Met 65 70 75 80 Pro Glu Glu Val Lys Glu Ser Ser Gln Leu Asp Lys Gln Glu Ser Leu 85 90 95 Gly Leu Glu Leu Lys Thr Ile Asn Ser Ala Gly Leu Gly Pro Ser Pro 100 105 110 Cys Leu Pro Asp Leu Asp Asp Phe Val Thr Arg Thr Ser Gly Val Gln 115 120 125 Lys Asp Lys Leu Cys Ser Pro Leu Ser Glu Pro Gly Asp Pro Ser Lys 130 135 140 Cys Ser Ser Leu Glu Leu Gly Pro Leu Gln Leu Glu Ile Ser Asn Ala 145 150 155 160 Ser Thr Thr Glu Val Ala Ile Leu Gln Val Asp Asp Asp Ser Gly Asp 165 170 175 Pro Leu Asn Leu Val Lys Ala Pro Val Ser Arg Ser Pro Pro Arg Glu 180 185 190 Gln Val Ile Glu Asp Asn Met Val Pro Gln Gly Met Pro Glu Gln Glu 195 200 205 Thr Thr Val Gly Ala Ile Gln Asp His Thr Glu Ser Ser Val His Asn 210 215 220 <210> 16 <211> 224 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2122 <400> 16 Glu Arg Ser Leu Ala Glu Met Glu Ser Ser His Gln Gln Val Met Glu 1 5 10 15 Glu Leu Gln Arg His His Glu Arg Glu Leu Gln Arg Leu Gln Gln Glu 20 25 30 Lys Glu Trp Leu Leu Ala Glu Glu Thr Ala Ala Thr Ala Ser Ala Ile 35 40 45 Glu Ala Met Lys Lys Ala Tyr Gln Glu Glu Leu Ser Arg Glu Leu Ser 50 55 60 Lys Thr Arg Ser Leu Gln Gln Gly Pro Asp Gly Leu Arg Lys Gln His 65 70 75 80 Gln Ser Asp Val Glu Ala Leu Lys Arg Glu Leu Gln Val Leu Ser Glu 85 90 95 Gln Tyr Ser Gln Lys Cys Leu Glu Ile Gly Ala Leu Met Arg Gln Ala 100 105 110 Glu Glu Arg Glu His Thr Leu Arg Arg Cys Gln Gln Glu Gly Gln Glu 115 120 125 Leu Leu Arg His Asn Gln Glu Leu His Gly Pro Pro Val Arg Gly Asp 130 135 140 Arg Pro Ala Ala Arg Leu His Cys Leu Ala Gly His Gly Gln Trp Leu 145 150 155 160 Arg Ala Gln Gln Arg Ala Glu Phe Leu Arg Ala Arg Gly Ala Ala Ser 165 170 175 Arg Lys Arg Lys Arg Thr Pro Val Pro Lys Glu Gly Gly Ala Val Pro 180 185 190 Pro Gly Arg Ala Pro Asp Asp Ala Glu Gly Gln Ala Leu His Leu Gly 195 200 205 Lys Val Pro Gly Arg Leu Cys Gly Ala Glu Pro His Gln Asp Thr Val 210 215 220 <210> 17 <211> 408 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2157 <400> 17 Arg Asn Pro Glu Ile Ser His Leu Leu Asn Asn Pro Asp Ile Met Arg 1 5 10 15 Gln Thr Leu Glu Ile Ala Arg Asn Pro Ala Met Met Gln Glu Met Met 20 25 30 Arg Asn Gln Asp Leu Ala Leu Ser Asn Leu Glu Ser Ile Pro Gly Gly 35 40 45 Tyr Asn Ala Leu Arg Arg Met Tyr Thr Asp Ile Gln Glu Pro Met Leu 50 55 60 Asn Ala Ala Gln Glu Gln Phe Gly Gly Asn Pro Phe Ala Ser Val Gly 65 70 75 80 Ser Ser Ser Ser Ser Gly Glu Gly Thr Gln Pro Ser Arg Thr Glu Asn 85 90 95 Arg Asp Pro Leu Pro Asn Pro Trp Ala Pro Pro Pro Ala Thr Gln Ser 100 105 110 Ser Ala Thr Thr Ser Thr Thr Thr Ser Thr Gly Ser Gly Ser Gly Asn 115 120 125 Ser Ser Ser Asn Ala Thr Gly Asn Thr Val Ala Ala Ala Asn Tyr Val 130 135 140 Ala Ser Ile Phe Ser Thr Pro Gly Met Gln Ser Leu Leu Gln Gln Ile 145 150 155 160 Thr Glu Asn Pro Gln Leu Ile Gln Asn Met Leu Ser Ala Pro Tyr Met 165 170 175 Arg Ser Met Met Gln Ser Leu Ser Gln Asn Pro Asp Leu Ala Ala Gln 180 185 190 Met Met Leu Asn Ser Pro Leu Phe Thr Ala Asn Pro Gln Leu Gln Glu 195 200 205 Gln Met Arg Pro Gln Leu Pro Ala Phe Leu Gln Gln Met Gln Asn Pro 210 215 220 Asp Thr Leu Ser Ala Met Ser Asn Pro Arg Ala Met Gln Ala Leu Met 225 230 235 240 Gln Ile Gln Gln Gly Leu Gln Thr Leu Ala Thr Glu Ala Pro Gly Leu 245 250 255 Ile Pro Ser Phe Thr Pro Gly Val Gly Val Gly Val Leu Gly Thr Ala 260 265 270 Ile Gly Pro Val Gly Pro Val Thr Pro Ile Gly Pro Ile Gly Pro Ile 275 280 285 Val Pro Phe Thr Pro Ile Gly Pro Ile Gly Pro Ile Gly Pro Thr Gly 290 295 300 Pro Ala Ala Pro Pro Gly Ser Thr Gly Ser Gly Gly Pro Thr Gly Pro 305 310 315 320 Thr Val Ser Ser Ala Ala Pro Arg Glu Thr Thr Ser Pro Thr Ser Glu 325 330 335 Ser Gly Pro Asn Gln Gln Phe Ile Gln Gln Met Val Gln Ala Leu Ala 340 345 350 Gly Ala Asn Ala Pro Gln Leu Pro Asn Pro Glu Val Arg Phe Gln Gln 355 360 365 Gln Leu Glu Gln Leu Asn Ala Met Gly Phe Leu Asn Arg Glu Ala Asn 370 375 380 Leu Gln Ala Leu Ile Ala Thr Gly Gly Asp Ile Asn Ala Ala Ile Glu 385 390 395 400 Arg Leu Leu Gly Ser Gln Pro Ser 405 <210> 18 <211> 486 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2216 <400> 18 Asn Ala Glu Arg Met Glu Ser Ile Leu Gln Ala Leu Glu Asp Ile Gln 1 5 10 15 Leu Asp Leu Glu Ala Val Asn Ile Lys Ala Gly Lys Ala Phe Leu Arg 20 25 30 Leu Lys Arg Lys Phe Ile Gln Met Arg Arg Pro Phe Leu Glu Arg Arg 35 40 45 Asp Leu Ile Ile Gln His Ile Pro Gly Phe Trp Val Lys Ala Phe Leu 50 55 60 Asn His Pro Arg Ile Ser Ile Leu Ile Asn Arg Arg Asp Glu Asp Ile 65 70 75 80 Phe Arg Tyr Leu Thr Asn Leu Gln Val Gln Asp Leu Arg His Ile Ser 85 90 95 Met Gly Tyr Lys Met Lys Leu Tyr Phe Gln Thr Asn Pro Tyr Phe Thr 100 105 110 Asn Met Val Ile Val Lys Glu Phe Gln Arg Asn Arg Ser Gly Arg Leu 115 120 125 Val Ser His Ser Thr Pro Ile Arg Trp His Arg Gly Gln Glu Pro Gln 130 135 140 Ala Arg Arg His Gly Asn Gln Asp Ala Ser His Ser Phe Phe Ser Trp 145 150 155 160 Phe Ser Asn His Ser Leu Pro Glu Ala Asp Arg Ile Ala Glu Ile Ile 165 170 175 Lys Asn Asp Leu Trp Val Asn Pro Leu Arg Tyr Tyr Leu Arg Glu Arg 180 185 190 Gly Ser Arg Ile Lys Arg Lys Lys Gln Glu Met Lys Lys Arg Lys Thr 195 200 205 Arg Gly Arg Cys Glu Val Val Ile Met Glu Asp Ala Pro Asp Tyr Tyr 210 215 220 Ala Val Glu Asp Ile Phe Ser Glu Ile Ser Asp Ile Asp Glu Thr Ile 225 230 235 240 His Asp Ile Lys Ile Ser Asp Phe Met Glu Thr Thr Asp Tyr Phe Glu 245 250 255 Thr Thr Asp Asn Glu Ile Thr Asp Ile Asn Glu Asn Ile Cys Asp Ser 260 265 270 Glu Asn Pro Asp His Asn Glu Val Pro Asn Asn Glu Thr Thr Asp Asn 275 280 285 Asn Glu Ser Ala Asp Asp His Glu Thr Thr Asp Asn Asn Glu Ser Ala 290 295 300 Asp Asp Asn Asn Glu Asn Pro Glu Asp Asn Asn Lys Asn Thr Asp Asp 305 310 315 320 Asn Glu Glu Asn Pro Asn Asn Asn Glu Asn Thr Tyr Gly Asn Asn Phe 325 330 335 Phe Lys Gly Gly Phe Trp Gly Ser His Gly Asn Asn Gln Asp Ser Ser 340 345 350 Asp Ser Asp Asn Glu Ala Asp Glu Ala Ser Asp Asp Glu Asp Asn Asp 355 360 365 Gly Asn Glu Gly Asp Asn Glu Gly Ser Asp Asp Asp Gly Asn Glu Gly 370 375 380 Asp Asn Glu Gly Ser Asp Asp Asp Asp Arg Asp Ile Glu Tyr Tyr Glu 385 390 395 400 Lys Gly Ile Glu Asp Phe Asp Arg Asp Gln Ala Asp Tyr Glu Asp Val 405 410 415 Ile Glu Ile Ile Ser Asp Glu Ser Val Glu Glu Glu Gly Ile Glu Glu 420 425 430 Gly Ile Gln Gln Asp Glu Asp Ile Tyr Glu Glu Gly Asn Tyr Glu Glu 435 440 445 Glu Gly Ser Glu Asp Val Trp Glu Glu Gly Glu Asp Ser Asp Asp Ser 450 455 460 Asp Leu Glu Asp Val Leu Gln Val Pro Asn Gly Trp Ala Asn Pro Gly 465 470 475 480 Lys Arg Gly Lys Thr Gly 485 <210> 19 <211> 67 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2025 <400> 19 Ile Ala Glu Thr Gly Val Ser Gly Gly Val Asp Arg Arg Glu Val Gln 1 5 10 15 Arg Leu Arg Arg Arg Asn Gln Gln Leu Glu Glu Glu Asn Asn Leu Leu 20 25 30 Arg Leu Lys Val Asp Ile Leu Leu Asp Met Leu Ser Glu Ser Thr Ala 35 40 45 Glu Ser His Leu Met Glu Lys Glu Leu Asp Glu Leu Arg Ile Ser Arg 50 55 60 Lys Arg Lys 65 <210> 20 <211> 108 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2281 <400> 20 Glu Gly Pro Leu Asp Ser Pro Ser Glu Gly His Ser Tyr Gln Asn Ser 1 5 10 15 Gly Leu Asp His Phe Gln Asn Ser Asn Ile Asp Gln Ser Phe Trp Glu 20 25 30 Thr Phe Gly Ser Ala Glu Pro Thr Lys Thr Arg Lys Ser Pro Ser Ser 35 40 45 Asp Ser Trp Thr Cys Ala Asp Thr Ser Thr Glu Arg Arg Ser Ser Asp 50 55 60 Ser Trp Glu Val Trp Gly Ser Ala Ser Thr Asn Arg Asn Ser Asn Ser 65 70 75 80 Asp Gly Gly Glu Gly Gly Glu Gly Thr Lys Lys Ala Val Pro Pro Ala 85 90 95 Val Pro Thr Asp Asp Gly Trp Asp Asn Gln Asn Trp 100 105 <210> 21 <211> 431 <212> PRT <213> Homo sapiens <220> <223> clone name: hfb2060 <400> 21 Asn Ser Pro Tyr Ser Leu Leu Glu Val Leu Cys Pro Arg Cys Asp Ile 1 5 10 15 Gly Leu Leu Gly Leu Lys Arg Lys Gln Arg Lys Leu Lys Glu Pro Leu 20 25 30 Pro Asn Gly Lys Lys Lys Ser Thr Lys Leu Glu Ser Leu Ser Asp Leu 35 40 45 Glu Arg Ala Leu Thr Glu Ser Lys Glu Thr Ala Ser Ala Met Ala Thr 50 55 60 Leu Gly Glu Ala Arg Leu Arg Glu Met Glu Ala Leu Gln Ser Leu Arg 65 70 75 80 Leu Ala Asn Glu Gly Lys Leu Leu Ser Pro Leu Gln Asp Val Asp Ile 85 90 95 Lys Ile Cys Leu Cys Gln Lys Ala Pro Ala Ala Pro Met Ile Gln Cys 100 105 110 Glu Leu Cys Arg Asp Ala Phe His Thr Ser Cys Val Ala Val Pro Ser 115 120 125 Ile Ser Gln Gly Leu Arg Ile Trp Leu Cys Pro His Cys Arg Arg Ser 130 135 140 Glu Lys Pro Pro Leu Glu Lys Ile Leu Pro Leu Leu Ala Ser Leu Gln 145 150 155 160 Arg Ile Arg Val Arg Leu Pro Glu Gly Asp Ala Leu Arg Tyr Met Ile 165 170 175 Glu Arg Thr Val Asn Trp Gln His Arg Ala Gln Gln Leu Leu Ser Ser 180 185 190 Gly Asn Leu Lys Phe Val Gln Asp Arg Val Gly Ser Gly Leu Leu Tyr 195 200 205 Ser Arg Trp Gln Ala Ser Ala Gly Gln Val Ser Asp Thr Asn Lys Val 210 215 220 Ser Gln Pro Pro Gly Thr Thr Ser Phe Ser Leu Pro Asp Asp Trp Asp 225 230 235 240 Asn Arg Thr Ser Tyr Leu His Ser Pro Phe Ser Thr Gly Arg Ser Cys 245 250 255 Ile Pro Leu His Gly Val Ser Pro Glu Val Asn Glu Leu Leu Met Glu 260 265 270 Ala Gln Leu Leu Gln Val Ser Leu Pro Glu Ile Gln Glu Leu Tyr Gln 275 280 285 Thr Leu Leu Ala Lys Pro Ser Pro Ala Gln Gln Thr Asp Arg Ser Ser 290 295 300 Pro Val Arg Pro Ser Ser Glu Lys Asn Asp Cys Cys Arg Gly Lys Arg 305 310 315 320 Asp Gly Ile Asn Ser Leu Glu Arg Lys Leu Lys Arg Arg Leu Glu Arg 325 330 335 Glu Gly Leu Ser Ser Glu Arg Trp Glu Arg Val Lys Lys Met Arg Thr 340 345 350 Pro Lys Lys Lys Lys Ile Lys Leu Ser His Pro Lys Asp Met Asn Asn 355 360 365 Phe Lys Leu Glu Arg Glu Arg Ser Tyr Glu Thr His Ser Leu Pro Ser 370 375 380 Asp Thr Ser Tyr Ser Glu Gln Glu Asp Ser Glu Asp Glu Asp Ala Ile 385 390 395 400 Cys Pro Ala Val Ser Cys Leu Gln Pro Glu Gly Asp Glu Val Ser Glu 405 410 415 Val Trp Ala Ile Glu Asp Val Leu Ser Leu Asn Ser Glu Thr Leu 420 425 430 <210> 22 <211> 1027 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2003 <220> <221> CDS <222> (1)..(783) <400> 22 ggc aag tcc tct gag gtc ctc tcg gcc acc gag ctc agg gtc cag ctg 48 Gly Lys Ser Ser Glu Val Leu Ser Ala Thr Glu Leu Arg Val Gln Leu 1 5 10 15 gcc cag aag gag cag gag cta gcc aga gcc aaa gaa gcc ttg cag gcc 96 Ala Gln Lys Glu Gln Glu Leu Ala Arg Ala Lys Glu Ala Leu Gln Ala 20 25 30 atg aaa gct gat cgg aag cgc tta aag ggc gag aag aca gac ctg gtg 144 Met Lys Ala Asp Arg Lys Arg Leu Lys Gly Glu Lys Thr Asp Leu Val 35 40 45 agc cag atg cag cag ctg tat gcc aca ctg gag agc cgc gag gag cag 192 Ser Gln Met Gln Gln Leu Tyr Ala Thr Leu Glu Ser Arg Glu Glu Gln 50 55 60 ctc cga gac ttc atc cgc aac tat gag cag cac cgc aag gag agc gag 240 Leu Arg Asp Phe Ile Arg Asn Tyr Glu Gln His Arg Lys Glu Ser Glu 65 70 75 80 gat gcg gtc aaa gcg ctg gcc aag gag aag gac ctg ctg gag cgt gag 288 Asp Ala Val Lys Ala Leu Ala Lys Glu Lys Asp Leu Leu Glu Arg Glu 85 90 95 aag tgg gag ctg cgg cgc caa gcc aag gag gcc aca gac cac gcc acg 336 Lys Trp Glu Leu Arg Arg Gln Ala Lys Glu Ala Thr Asp His Ala Thr 100 105 110 gca ctg cgc tcc cag ctg gac ctc aag gac aac cgg atg aag gag ctg 384 Ala Leu Arg Ser Gln Leu Asp Leu Lys Asp Asn Arg Met Lys Glu Leu 115 120 125 gag gcc gag ctg gcc atg gcc aaa cag tcc tta gct acg ctg acc aag 432 Glu Ala Glu Leu Ala Met Ala Lys Gln Ser Leu Ala Thr Leu Thr Lys 130 135 140 gac gtc ccc aag cgg cat tcc ctc gcc atg ccg ggc gag acg gtg ctc 480 Asp Val Pro Lys Arg His Ser Leu Ala Met Pro Gly Glu Thr Val Leu 145 150 155 160 aat ggc aac cag gag tgg gtg gtg cag gcg gac ctc ccg ctg acc gca 528 Asn Gly Asn Gln Glu Trp Val Val Gln Ala Asp Leu Pro Leu Thr Ala 165 170 175 gcc atc cgg cag agt caa cag act ctc tac cac tca cac ccc cct cac 576 Ala Ile Arg Gln Ser Gln Gln Thr Leu Tyr His Ser His Pro Pro His 180 185 190 cct gcg gac cgg caa gcg gtc agg gtg agc ccc tgc cac tcc cgg cag 624 Pro Ala Asp Arg Gln Ala Val Arg Val Ser Pro Cys His Ser Arg Gln 195 200 205 ccc tct gtc atc tcc gac gca tct gcc gcc gaa ggc gac cgg tcg tcc 672 Pro Ser Val Ile Ser Asp Ala Ser Ala Ala Glu Gly Asp Arg Ser Ser 210 215 220 aca ccg agc gac atc aac tcc cct cga cac cgg aca cac tcc ctc tgg 720 Thr Pro Ser Asp Ile Asn Ser Pro Arg His Arg Thr His Ser Leu Trp 225 230 235 240 cga cag tcc cgg ccc agt tca gaa gaa cct gca caa ccc tat tgt aca 768 Arg Gln Ser Arg Pro Ser Ser Glu Glu Pro Ala Gln Pro Tyr Cys Thr 245 250 255 gtc act aga gga tct tgaagaccaa aaacggaaaa agaagaaaga gaagatggga 823 Val Thr Arg Gly Ser 260 ttcggctcca tctcccgcgt cttcgccaga gggaagcagc ggaagtccct cgaccccggc 883 ctctttgatg gtaccgcccc tgattattac atagaggagg acgcggactg gtgatacgcg 943 ctcccctgcg cctgctgccc gcaggcgtgt ctgtgcgtgt gggcgtgtgt gcaagcgagc 1003 gtgggtgcgc gtgtggcccg tgcg 1027 <210> 23 <211> 865 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2007 <220> <221> CDS <222> (1)..(759) <400> 23 gac tat gag gag atc acc atc gac ccc acg tgc agc tgg aag cca gtg 48 Asp Tyr Glu Glu Ile Thr Ile Asp Pro Thr Cys Ser Trp Lys Pro Val 1 5 10 15 ccc gtg aag cct gac atg cac atc aag gag gag ccg gat ggg cca gca 96 Pro Val Lys Pro Asp Met His Ile Lys Glu Glu Pro Asp Gly Pro Ala 20 25 30 ctg aag cgc tgc cgc acc gtg agc ccc gcc cac gtg ctc atg ccc agc 144 Leu Lys Arg Cys Arg Thr Val Ser Pro Ala His Val Leu Met Pro Ser 35 40 45 gtg atg gag atg atc gcc gcc ctg ggc ccc ggc gct gcc ccc ttt gcc 192 Val Met Glu Met Ile Ala Ala Leu Gly Pro Gly Ala Ala Pro Phe Ala 50 55 60 ccc ctg cag ccc ccc tca gtc cct gcc ccc agc gac tac cct ggc cag 240 Pro Leu Gln Pro Pro Ser Val Pro Ala Pro Ser Asp Tyr Pro Gly Gln 65 70 75 80 ggt tcc agc ttc ctg ggg cct gga act ttc cct gag tcc ttc cca ccc 288 Gly Ser Ser Phe Leu Gly Pro Gly Thr Phe Pro Glu Ser Phe Pro Pro 85 90 95 acc acg ccc agc acc cca acc ctt gct gag ttc acc ccg gga cca ccc 336 Thr Thr Pro Ser Thr Pro Thr Leu Ala Glu Phe Thr Pro Gly Pro Pro 100 105 110 ccc atc tgc tac cag tct gac att ccc agc agc ctc ctg act tca gag 384 Pro Ile Cys Tyr Gln Ser Asp Ile Pro Ser Ser Leu Leu Thr Ser Glu 115 120 125 aag tct acc gtc tgc ctc cca agc cag atg gca cca gca ggt cac ctg 432 Lys Ser Thr Val Cys Leu Pro Ser Gln Met Ala Pro Ala Gly His Leu 130 135 140 gac ccc act cac aat cct ggg aca cca gga cta cac acc tcc aac ctt 480 Asp Pro Thr His Asn Pro Gly Thr Pro Gly Leu His Thr Ser Asn Leu 145 150 155 160 ggg gcc cct cca ggt ccc cag ctg cac cat tca aac cct ccc cca gcg 528 Gly Ala Pro Pro Gly Pro Gln Leu His His Ser Asn Pro Pro Pro Ala 165 170 175 tcc cgg cag tcc ttg ggc caa gcg agc tta gga cct acg ggt gaa ctg 576 Ser Arg Gln Ser Leu Gly Gln Ala Ser Leu Gly Pro Thr Gly Glu Leu 180 185 190 gcc ttc agt cct gcc aca ggc gtg atg ggg ccc ccc agc atg tct gga 624 Ala Phe Ser Pro Ala Thr Gly Val Met Gly Pro Pro Ser Met Ser Gly 195 200 205 gcc ggg gag gcc cca gaa cca gct ctg gac ctg ctc ccg gaa ctg acc 672 Ala Gly Glu Ala Pro Glu Pro Ala Leu Asp Leu Leu Pro Glu Leu Thr 210 215 220 aac cct gat gag cta ctg tcc tac ttg ggc cca ccc gac ctc cct acg 720 Asn Pro Asp Glu Leu Leu Ser Tyr Leu Gly Pro Pro Asp Leu Pro Thr 225 230 235 240 aac aac aat gac gac ctg ctt tct ctg ttt gag aac aac tgatcctgtg 769 Asn Asn Asn Asp Asp Leu Leu Ser Leu Phe Glu Asn Asn 245 250 tttaccccaa gcccggcggg gacacgctca cagatggcac cacaggcctg cccttcatgc 829 ccagccccat gggacacccg gtggtctttc ccaaac 865 <210> 24 <211> 1064 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2017 <220> <221> CDS <222> (1)..(648) <400> 24 aaa agt gaa agc aat gag gac ctt ttt ggg ctt gtg ccc ttt gat gaa 48 Lys Ser Glu Ser Asn Glu Asp Leu Phe Gly Leu Val Pro Phe Asp Glu 1 5 10 15 ata acg ggg agc cag cag caa aaa gtc aaa cag cgc agc tta cag aaa 96 Ile Thr Gly Ser Gln Gln Gln Lys Val Lys Gln Arg Ser Leu Gln Lys 20 25 30 ctg tcc tct cgc caa agg cgc aca aag cag gat atg tcc aaa agt aat 144 Leu Ser Ser Arg Gln Arg Arg Thr Lys Gln Asp Met Ser Lys Ser Asn 35 40 45 ggg aag cgg cat cat ggc acg cca act agc aca aag aag act ttg aag 192 Gly Lys Arg His His Gly Thr Pro Thr Ser Thr Lys Lys Thr Leu Lys 50 55 60 cct acc tat cgc act cca gag agg gct cgc agg cac aaa aaa gtg ggc 240 Pro Thr Tyr Arg Thr Pro Glu Arg Ala Arg Arg His Lys Lys Val Gly 65 70 75 80 cgc cga gac tct caa agt agc aat gaa ttt tta acc atc tca gac tcc 288 Arg Arg Asp Ser Gln Ser Ser Asn Glu Phe Leu Thr Ile Ser Asp Ser 85 90 95 aag gag aac att agt gtt gca ctg act gat ggg aaa gat agg ggg aat 336 Lys Glu Asn Ile Ser Val Ala Leu Thr Asp Gly Lys Asp Arg Gly Asn 100 105 110 gtc tta caa cct gag gag agc ctg ttg gac ccc ttc ggt gcc aag ccc 384 Val Leu Gln Pro Glu Glu Ser Leu Leu Asp Pro Phe Gly Ala Lys Pro 115 120 125 ttc cat tct cca gac ctg tca tgg cac cct cca cat cag ggc ctg agc 432 Phe His Ser Pro Asp Leu Ser Trp His Pro Pro His Gln Gly Leu Ser 130 135 140 gac atc cgt gct gat cac aat act gtc ctg cca ggg cgg cca aga caa 480 Asp Ile Arg Ala Asp His Asn Thr Val Leu Pro Gly Arg Pro Arg Gln 145 150 155 160 aat tca cta cat ggg tca ttc cat agt gca gat gta ttg aaa atg gat 528 Asn Ser Leu His Gly Ser Phe His Ser Ala Asp Val Leu Lys Met Asp 165 170 175 gat ttt ggt gcc gtg ccc ttt aca gaa ctt gtg gtg caa agc atc act 576 Asp Phe Gly Ala Val Pro Phe Thr Glu Leu Val Val Gln Ser Ile Thr 180 185 190 cca cat cag tcc caa cag tcc caa cca gtc gaa tta gac cca ttt ggt 624 Pro His Gln Ser Gln Gln Ser Gln Pro Val Glu Leu Asp Pro Phe Gly 195 200 205 gct gct cca ttt cct tct aaa cag tagatacttc tgatggattc tcggcattaa 678 Ala Ala Pro Phe Pro Ser Lys Gln 210 215 ctcctgtttc aaaaaagtgt gaacagtttt atgaatttga aagaaaattt ggtagctctt 738 tatagcattc attcttaaag atcagtcaga ataggtgatt tctaaataaa ccaaatagaa 798 gaatgaagta tctctacagg gtagtaactt gattcctctt caggagaaaa gggagctaaa 858 ttgcaagctc taactaaggg tttctgctac tgacatcaca acacagaaat gcaagtgtgg 918 tacttccagt gaaagcacat ggcacctttc taggtgtgta gccactgaga agggacagtg 978 aaactgttat ttttgatatc agaatgtcat ttttatgtgc atatccctaa aattagggtt 1038 atttctacat acactagttc acttgt 1064 <210> 25 <211> 1453 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2018 <220> <221> CDS <222> (1)..(1119) <400> 25 gag cag cag aag ccg gcg tcg tcg gat gtt gtg ttg ccc gcc acc atg 48 Glu Gln Gln Lys Pro Ala Ser Ser Asp Val Val Leu Pro Ala Thr Met 1 5 10 15 agc tac aca ggc ttt gtc cag gga tct gaa acc act ttg cag tcg aca 96 Ser Tyr Thr Gly Phe Val Gln Gly Ser Glu Thr Thr Leu Gln Ser Thr 20 25 30 tac tcg gat acc agc gct cag ccc acc tgt gat tat gga tat gga act 144 Tyr Ser Asp Thr Ser Ala Gln Pro Thr Cys Asp Tyr Gly Tyr Gly Thr 35 40 45 tgg aac tct ggg aca aat aga ggc tac gag ggc tat ggc tat ggc tat 192 Trp Asn Ser Gly Thr Asn Arg Gly Tyr Glu Gly Tyr Gly Tyr Gly Tyr 50 55 60 ggc tat ggc cag gat aac acc acc aac tat ggg tat ggt atg gcc act 240 Gly Tyr Gly Gln Asp Asn Thr Thr Asn Tyr Gly Tyr Gly Met Ala Thr 65 70 75 80 tca cac tct tgg gaa atg cct agc tct gac aca aat gca aac act agt 288 Ser His Ser Trp Glu Met Pro Ser Ser Asp Thr Asn Ala Asn Thr Ser 85 90 95 gcc tcg ggt agc gcc agt gcc gat tcc gtt tta tcc aga att aac cag 336 Ala Ser Gly Ser Ala Ser Ala Asp Ser Val Leu Ser Arg Ile Asn Gln 100 105 110 cgc tta gat atg gtg ccg cat ttg gag aca gac atg atg caa gga ggc 384 Arg Leu Asp Met Val Pro His Leu Glu Thr Asp Met Met Gln Gly Gly 115 120 125 gtg tac ggc tca ggt gga gaa agg tat gac tct tat gag tcc tgc gac 432 Val Tyr Gly Ser Gly Gly Glu Arg Tyr Asp Ser Tyr Glu Ser Cys Asp 130 135 140 tcg agg gcc gtc ctg agt gag cgc gac ctg tac cgg tca ggc tat gac 480 Ser Arg Ala Val Leu Ser Glu Arg Asp Leu Tyr Arg Ser Gly Tyr Asp 145 150 155 160 tac agc gag ctt gac cct gag atg gaa atg gcc tat gag ggc caa tac 528 Tyr Ser Glu Leu Asp Pro Glu Met Glu Met Ala Tyr Glu Gly Gln Tyr 165 170 175 gat gcc tac cgc gac cag ttc cgc atg cgt ggc aac gac acc ttc ggt 576 Asp Ala Tyr Arg Asp Gln Phe Arg Met Arg Gly Asn Asp Thr Phe Gly 180 185 190 ccc agg gca cag ggc tgg gcc cgg gat gcc cgg agc ggc cgg cca atg 624 Pro Arg Ala Gln Gly Trp Ala Arg Asp Ala Arg Ser Gly Arg Pro Met 195 200 205 gcc tca ggc tat ggg cgc atg tgg gaa gac ccc atg ggg gcc cgg ggc 672 Ala Ser Gly Tyr Gly Arg Met Trp Glu Asp Pro Met Gly Ala Arg Gly 210 215 220 cag tgc atg tct ggt gcc tct cgg ctg ccc tcc ctc ttc tcc cag aac 720 Gln Cys Met Ser Gly Ala Ser Arg Leu Pro Ser Leu Phe Ser Gln Asn 225 230 235 240 atc atc ccc gag tac ggc atg ttc cag ggc atg cga ggt ggg ggc gcc 768 Ile Ile Pro Glu Tyr Gly Met Phe Gln Gly Met Arg Gly Gly Gly Ala 245 250 255 ttc ccg ggc ggc tcc cgc ttt ggt ttc ggg ttt ggc aat ggc atg aag 816 Phe Pro Gly Gly Ser Arg Phe Gly Phe Gly Phe Gly Asn Gly Met Lys 260 265 270 cag atg agg cgg acc tgg aag acc tgg acc aca gcc gac ttc cga acc 864 Gln Met Arg Arg Thr Trp Lys Thr Trp Thr Thr Ala Asp Phe Arg Thr 275 280 285 aag aag aag aag aga aag cag ggc ggc agt cct gat gag cca gat agc 912 Lys Lys Lys Lys Arg Lys Gln Gly Gly Ser Pro Asp Glu Pro Asp Ser 290 295 300 aaa gcc acc cgc acg gac tgc tcg gac aac agc gac tca gac aat gat 960 Lys Ala Thr Arg Thr Asp Cys Ser Asp Asn Ser Asp Ser Asp Asn Asp 305 310 315 320 gag ggc acc gag ggg gaa gcc aca gag ggc ctt gaa ggc acc gag gct 1008 Glu Gly Thr Glu Gly Glu Ala Thr Glu Gly Leu Glu Gly Thr Glu Ala 325 330 335 gtg gag aag ggc tcc aga gtg gac gga gag gat gag gag gga aaa gag 1056 Val Glu Lys Gly Ser Arg Val Asp Gly Glu Asp Glu Glu Gly Lys Glu 340 345 350 gat ggg aga gaa gaa ggc aaa gag gat cca gag aag ggt gag ttt tcc 1104 Asp Gly Arg Glu Glu Gly Lys Glu Asp Pro Glu Lys Gly Glu Phe Ser 355 360 365 tgg ggt gcc tgg tgc tagggcggct cttccagggg ctcacagtgg gagggaccca 1159 Trp Gly Ala Trp Cys 370 gctgagggca gggttgcggc agacatgcgg ccagttgggg acctcccttg tgggcccgag 1219 tgtgtgctgg cagggggctt tcttttcgcc gcctccaccc ccttcccact cttcctcctc 1279 cttcccaggc ggaggctgtg gatggtggtg acccctgcct agcacactgg ttttgccttc 1339 agatgggggc agctagtaaa agtgtgggga accccaggca cgccccctgc cctcggtccc 1399 tcagtaacgc gcagttccaa gtcctcagtg gctggtgtcc gatctgggac cctc 1453 <210> 26 <211> 1156 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2038 <220> <221> CDS <222> (1)..(567) <400> 26 gag tta ttt gcc cgt tat gac cgc ctt ctc ctc cag tcc tcc ttg gac 48 Glu Leu Phe Ala Arg Tyr Asp Arg Leu Leu Leu Gln Ser Ser Leu Asp 1 5 10 15 ctg atg gca gat gtg gtg tac tgc ccc cgg ccg tgc tgc cag ctg cct 96 Leu Met Ala Asp Val Val Tyr Cys Pro Arg Pro Cys Cys Gln Leu Pro 20 25 30 gtg atg cag gaa cct ggc tgc acc atg ggt atc tgc tcc agc tgc aat 144 Val Met Gln Glu Pro Gly Cys Thr Met Gly Ile Cys Ser Ser Cys Asn 35 40 45 ttt gcc ttc tgt act ttg tgc agg ttg acc tac cat ggg gtc tcc cca 192 Phe Ala Phe Cys Thr Leu Cys Arg Leu Thr Tyr His Gly Val Ser Pro 50 55 60 tgt aag gtg act gca gag aaa tta atg gac tta cga aat gaa tac ctg 240 Cys Lys Val Thr Ala Glu Lys Leu Met Asp Leu Arg Asn Glu Tyr Leu 65 70 75 80 caa gcg gat gag gct aat aaa aga ctt ttg gat caa agg tat ggt aag 288 Gln Ala Asp Glu Ala Asn Lys Arg Leu Leu Asp Gln Arg Tyr Gly Lys 85 90 95 aga gtg att cag aag gca ctg gaa gag atg gaa agt aag gag tgg cta 336 Arg Val Ile Gln Lys Ala Leu Glu Glu Met Glu Ser Lys Glu Trp Leu 100 105 110 gag aag aac tca aag agc tgc cca tgt tgt gga act ccc ata gag aaa 384 Glu Lys Asn Ser Lys Ser Cys Pro Cys Cys Gly Thr Pro Ile Glu Lys 115 120 125 tta gac gga tgt aac aag atg aca tgt act ggc tgt atg caa tat ttc 432 Leu Asp Gly Cys Asn Lys Met Thr Cys Thr Gly Cys Met Gln Tyr Phe 130 135 140 tgt tgg att tgc atg ggt tct ctc tct aga gca aac cct tac aaa cat 480 Cys Trp Ile Cys Met Gly Ser Leu Ser Arg Ala Asn Pro Tyr Lys His 145 150 155 160 ttc aat gac cct ggt tca cca tgt ttt aac cgg ctg ttt tat gct gtg 528 Phe Asn Asp Pro Gly Ser Pro Cys Phe Asn Arg Leu Phe Tyr Ala Val 165 170 175 gat gtt gac gac gat att tgg gaa gat gag gta gaa gac tagttaacta 577 Asp Val Asp Asp Asp Ile Trp Glu Asp Glu Val Glu Asp 180 185 ctgctcaaga tatggaagtg gattgttttt ccctaatctt ccgtcaagta cacaaagtaa 637 ctttgcggga tatttagggt actattcatt cactcttcct gcgtagaaga tatggaagaa 697 cgaggtttat attttcatgt ggtactactg aagaaggtgc attgatacat ttttaaatgt 757 aagttgagaa aaatttataa gccaaaggtt cagaaaatta aactacagaa tattaaatat 817 tataatgtgc ccaaagctct gaatagttaa aaattaaata tttattttct tccccaagct 877 ttaggtaagg agaagagggg tcaagagtta aacttagaga ccctttgtct ctgagaagca 937 tccttctaag acattctgtt ggagttccct cagtactatt ccttacaact ggagtgggta 997 gaagccttat gaaaattata ctgacaacct gatctcgttt actccatgtt aatcacattc 1057 ctaccaacct aaatttctgc caagtcagtt ctctttggag gaagcccatt actccataac 1117 tgactggatg gtccagtgtc atttttgatc tgctttttc 1156 <210> 27 <211> 465 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2099 <220> <221> CDS <222> (1)..(369) <400> 27 gac gcg tgg gcc agc gtc ggc tgt ccc tcc agc tcc cca tcc tcc acc 48 Asp Ala Trp Ala Ser Val Gly Cys Pro Ser Ser Ser Pro Ser Ser Thr 1 5 10 15 acg cct acc tcc cat cca tcg gag gcg tgg acg cca gct gtg tca gcc 96 Thr Pro Thr Ser His Pro Ser Glu Ala Trp Thr Pro Ala Val Ser Ala 20 25 30 cct gcg tca gcc cca ccg cca gcc ccc gcc aca gac atg tgc cac cct 144 Pro Ala Ser Ala Pro Pro Pro Ala Pro Ala Thr Asp Met Cys His Pro 35 40 45 cct tcc gag tca tgg tct cgg gcc tgt aag ggt ggg agg cct ggg ccc 192 Pro Ser Glu Ser Trp Ser Arg Ala Cys Lys Gly Gly Arg Pro Gly Pro 50 55 60 ggg gcc tcc ccc gtg aca gaa cca cac tgg gca gag ggg tct gct gca 240 Gly Ala Ser Pro Val Thr Glu Pro His Trp Ala Glu Gly Ser Ala Ala 65 70 75 80 gaa aca ctg tcg gct ctg gct gcg gag aag ctg ggc acc atg gct ggc 288 Glu Thr Leu Ser Ala Leu Ala Ala Glu Lys Leu Gly Thr Met Ala Gly 85 90 95 ctc tca gga cca ctc gga tgg cac tca ggt gga cag gac ggg gca ggg 336 Leu Ser Gly Pro Leu Gly Trp His Ser Gly Gly Gln Asp Gly Ala Gly 100 105 110 gga gac ttg gca cct gac ctc gag cct tat ttg tgaagtcctt atttcttcac 389 Gly Asp Leu Ala Pro Asp Leu Glu Pro Tyr Leu 115 120 aaagaagagg aacggaaatg ggacgtcttc cttaacatct gcaaacaagg aggcgctggg 449 atatcaaact tgcaat 465 <210> 28 <211> 464 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2072 <220> <221> CDS <222> (1)..(375) <400> 28 tgc cgg ggt cag ttg gtc caa gtg tcc cgg cct gag gtg tcg gcc gga 48 Cys Arg Gly Gln Leu Val Gln Val Ser Arg Pro Glu Val Ser Ala Gly 1 5 10 15 tcc ctc ctt ctc ccg gcg cct caa gcg gaa gac cat tcc tca aga att 96 Ser Leu Leu Leu Pro Ala Pro Gln Ala Glu Asp His Ser Ser Arg Ile 20 25 30 ttg tat cca agg ccc aaa agt ttg tta ccc aag atg atg aat gct gac 144 Leu Tyr Pro Arg Pro Lys Ser Leu Leu Pro Lys Met Met Asn Ala Asp 35 40 45 atg gat gca gtt gat gct gaa aat caa gtg gaa ctg gag gaa aaa aca 192 Met Asp Ala Val Asp Ala Glu Asn Gln Val Glu Leu Glu Glu Lys Thr 50 55 60 aga ctt att aat caa gtg ttg gaa ctc caa cac aca ctt gaa gat ctc 240 Arg Leu Ile Asn Gln Val Leu Glu Leu Gln His Thr Leu Glu Asp Leu 65 70 75 80 tct gca aga gta gat gca gtt aag gaa gaa aat ctg aag cta aaa tca 288 Ser Ala Arg Val Asp Ala Val Lys Glu Glu Asn Leu Lys Leu Lys Ser 85 90 95 gaa aac caa gtt ctt gga caa tat ata gaa aat ctc atg tca gct tct 336 Glu Asn Gln Val Leu Gly Gln Tyr Ile Glu Asn Leu Met Ser Ala Ser 100 105 110 agt gtt ttt caa aca act gac aca aaa agc aaa aga aag taagggattg 385 Ser Val Phe Gln Thr Thr Asp Thr Lys Ser Lys Arg Lys 115 120 125 acacccttct gttttatgga attgctgctg atcatttttt ctttaaaact tggatagatt 445 ccaaaagtta cagtacctt 464 <210> 29 <211> 1436 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2091 <220> <221> CDS <222> (1)..(1128) <400> 29 aag atg gat gtt ctt cag ttc cta gaa gga atc cca gtg gat gaa aat 48 Lys Met Asp Val Leu Gln Phe Leu Glu Gly Ile Pro Val Asp Glu Asn 1 5 10 15 gct gta cat gtt ctt gtt gat aac aat ggg caa ggt cta gga cag gca 96 Ala Val His Val Leu Val Asp Asn Asn Gly Gln Gly Leu Gly Gln Ala 20 25 30 ttg gtt cag ttt aaa aat gaa gat gat gca cgt aag tct gaa cgc tta 144 Leu Val Gln Phe Lys Asn Glu Asp Asp Ala Arg Lys Ser Glu Arg Leu 35 40 45 cac cgt aaa aaa ctt aat ggg aga gaa gct ttt gtt cat gta gtt acc 192 His Arg Lys Lys Leu Asn Gly Arg Glu Ala Phe Val His Val Val Thr 50 55 60 cta gaa gat atg aga gag att gag aaa aat ccc cct gcc caa gga aaa 240 Leu Glu Asp Met Arg Glu Ile Glu Lys Asn Pro Pro Ala Gln Gly Lys 65 70 75 80 aag gga tta aag atg cct gtg cca ggt aat cct gca gtt cca gga atg 288 Lys Gly Leu Lys Met Pro Val Pro Gly Asn Pro Ala Val Pro Gly Met 85 90 95 ccc aat gcg gga ctg ccc ggt gtg gga ctg ccc agt gca gga ctt ccc 336 Pro Asn Ala Gly Leu Pro Gly Val Gly Leu Pro Ser Ala Gly Leu Pro 100 105 110 ggt gca ggc ctg ccc agc aca gga ctg cct ggt tca gca ata acc agt 384 Gly Ala Gly Leu Pro Ser Thr Gly Leu Pro Gly Ser Ala Ile Thr Ser 115 120 125 gca gga ctg cct ggt gcg gga atg ccc agt gca gga ata cct agt gca 432 Ala Gly Leu Pro Gly Ala Gly Met Pro Ser Ala Gly Ile Pro Ser Ala 130 135 140 gga ggt gaa gag cat gcc ttc ctg act gta gga tca aag gaa gcc aat 480 Gly Gly Glu Glu His Ala Phe Leu Thr Val Gly Ser Lys Glu Ala Asn 145 150 155 160 aat ggg cct cca ttt aac ttt cct ggt aat ttt ggt gga tca aat gcc 528 Asn Gly Pro Pro Phe Asn Phe Pro Gly Asn Phe Gly Gly Ser Asn Ala 165 170 175 ttt ggg cca cca atc cct cct cca gga tta gga ggc ggg gcc ttt ggt 576 Phe Gly Pro Pro Ile Pro Pro Pro Gly Leu Gly Gly Gly Ala Phe Gly 180 185 190 gat gct agg cct ggt atg cct tca gtt gga aac agt ggt ttg cct ggt 624 Asp Ala Arg Pro Gly Met Pro Ser Val Gly Asn Ser Gly Leu Pro Gly 195 200 205 cta gga ctg gat gtt ccg ggt ttt gga ggt gga cca aac aat tta agt 672 Leu Gly Leu Asp Val Pro Gly Phe Gly Gly Gly Pro Asn Asn Leu Ser 210 215 220 ggg cca tcg gga ttt gga ggg ggc cct cag aat ttt gga aat ggc cct 720 Gly Pro Ser Gly Phe Gly Gly Gly Pro Gln Asn Phe Gly Asn Gly Pro 225 230 235 240 ggt agc tta ggc ggt ccc ccg ggg ttt gga agt ggc cct cct ggt ctt 768 Gly Ser Leu Gly Gly Pro Pro Gly Phe Gly Ser Gly Pro Pro Gly Leu 245 250 255 gga agt gcc cct ggg cat ttg ggt ggg cca cca gct ttt ggg cct ggc 816 Gly Ser Ala Pro Gly His Leu Gly Gly Pro Pro Ala Phe Gly Pro Gly 260 265 270 ccc ggc ccc ggc ccc ggc cct ggc cca atc cat att ggt ggt ccc cct 864 Pro Gly Pro Gly Pro Gly Pro Gly Pro Ile His Ile Gly Gly Pro Pro 275 280 285 ggc ttt gca tct agt tct gga aaa cca gga ccg aca gta att aaa gtg 912 Gly Phe Ala Ser Ser Ser Gly Lys Pro Gly Pro Thr Val Ile Lys Val 290 295 300 caa aac atg ccc ttt act gtg tct att gat gag att tta gat ttc ttt 960 Gln Asn Met Pro Phe Thr Val Ser Ile Asp Glu Ile Leu Asp Phe Phe 305 310 315 320 tat ggc tat caa gta atc cca ggc tca gtg tgt tta aaa tac aat gaa 1008 Tyr Gly Tyr Gln Val Ile Pro Gly Ser Val Cys Leu Lys Tyr Asn Glu 325 330 335 aaa ggt atg ccc aca ggt gaa gcc atg gtg gcc ttt gag tct cgg gat 1056 Lys Gly Met Pro Thr Gly Glu Ala Met Val Ala Phe Glu Ser Arg Asp 340 345 350 gaa gcc aca gct gct gtc att gac tta aat gac agg cct ata ggt tca 1104 Glu Ala Thr Ala Ala Val Ile Asp Leu Asn Asp Arg Pro Ile Gly Ser 355 360 365 aga aaa gta aaa ctt gta tta ggg tagccattca catcattttt tatagggtag 1158 Arg Lys Val Lys Leu Val Leu Gly 370 375 atcttcatat tgctgtgatt aatgcatcca gattgttttc ctagtatttc caggttagaa 1218 cctgtggatt gtttcaattg catatagctt ggtttccata acatagagca ttggttgact 1278 gtttacagaa gactcactca ccaggataaa cattgctgta tgttacagta aagctatctg 1338 gagagaacac ataaatgatt ttggcatacc attagagaaa ccatttgtaa aactcaaatg 1398 accacataaa gcttatcaag gagtctagat tggttttg 1436 <210> 30 <211> 537 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2115 <220> <221> CDS <222> (1)..(315) <400> 30 gaa gac cta gga aag gac gtt gaa ttt gaa gtt gtt ggt gat gcc ccg 48 Glu Asp Leu Gly Lys Asp Val Glu Phe Glu Val Val Gly Asp Ala Pro 1 5 10 15 gaa aaa gtg ggg ccc aaa caa gct gaa gat gct gcc aaa agc ata acc 96 Glu Lys Val Gly Pro Lys Gln Ala Glu Asp Ala Ala Lys Ser Ile Thr 20 25 30 aat ggc agt gat gat gga gct cag ccc tcc acc tcc aca gct caa gag 144 Asn Gly Ser Asp Asp Gly Ala Gln Pro Ser Thr Ser Thr Ala Gln Glu 35 40 45 caa gat gac gtt ctc ata gtt gat tcg gat gaa gaa gat tct tca aat 192 Gln Asp Asp Val Leu Ile Val Asp Ser Asp Glu Glu Asp Ser Ser Asn 50 55 60 aat gcc gac gtc agt gaa gaa gag aga agc cgc aag agg aaa tta gat 240 Asn Ala Asp Val Ser Glu Glu Glu Arg Ser Arg Lys Arg Lys Leu Asp 65 70 75 80 gag aaa gag aat ctc agt gca aag agg tca cgt ata gaa cag aag gaa 288 Glu Lys Glu Asn Leu Ser Ala Lys Arg Ser Arg Ile Glu Gln Lys Glu 85 90 95 gag ctt gat gat gtc ata gca tta gat tgaacagaaa tgcctctaaa 335 Glu Leu Asp Asp Val Ile Ala Leu Asp 100 105 cagaaccctc ttactattta gtttatctgg gcagaaccag attgttatgt cctttgttcc 395 aaagggaaaa aattgacagc agtgacttga aaatgattct gctccctttg aaagcattca 455 ttttgctaga actggtagac acattgcagt atgctgtatt gaaagtagga atatagtttt 515 aaaaaccctt tgacaaagtg tg 537 <210> 31 <211> 536 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2206 <220> <221> CDS <222> (1)..(264) <400> 31 ccc cag ttg gtg gtg gta ggg gaa gag ggc ttc cct gat act ggc tcc 48 Pro Gln Leu Val Val Val Gly Glu Glu Gly Phe Pro Asp Thr Gly Ser 1 5 10 15 gac cat tcg tac tcc ttg tcg tca ggc acc acg gag gag gag ctc ctg 96 Asp His Ser Tyr Ser Leu Ser Ser Gly Thr Thr Glu Glu Glu Leu Leu 20 25 30 cgc aag ctg aat gag cag cgg gac atc ctg gct ctg atg gaa gtg aag 144 Arg Lys Leu Asn Glu Gln Arg Asp Ile Leu Ala Leu Met Glu Val Lys 35 40 45 atg aaa gag atg aaa ggc agc att cgc cac ctg cgt ctc act gag gcc 192 Met Lys Glu Met Lys Gly Ser Ile Arg His Leu Arg Leu Thr Glu Ala 50 55 60 aag ctg cgc gaa gaa ctg cgt gag aag gat cgg ctg ctt gcc atg gct 240 Lys Leu Arg Glu Glu Leu Arg Glu Lys Asp Arg Leu Leu Ala Met Ala 65 70 75 80 gtc atc cgc aag aag cac gga atg tgaactggtg ccccggcagc ctgctggact 294 Val Ile Arg Lys Lys His Gly Met 85 cccagacccc atccagccag gggaccgcag gccattgttg aactcctcta tactcctggg 354 cactggttga cagtactgag gcttaaggca gctggactct cttgctggtg acctggcatc 414 ctcaattgtt tctcctgaag tggaagctgg ggccttagac tctgcctggt gacaccagca 474 attatgactt tgctaccctt cctcccagtt attggtgcag attctggtta agcagaagct 534 tc 536 <210> 32 <211> 952 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2255 <220> <221> CDS <222> (1)..(909) <400> 32 cta att tct gac ctc aag atg gaa cga gag gcg aga gaa aat gga agc 48 Leu Ile Ser Asp Leu Lys Met Glu Arg Glu Ala Arg Glu Asn Gly Ser 1 5 10 15 aag tct cct gca cat agt tac tcc agc tat gac tct ggc aaa aat gag 96 Lys Ser Pro Ala His Ser Tyr Ser Ser Tyr Asp Ser Gly Lys Asn Glu 20 25 30 agt gta gac cga gga gct gag gac ctc tca cta aac agg gga gat gag 144 Ser Val Asp Arg Gly Ala Glu Asp Leu Ser Leu Asn Arg Gly Asp Glu 35 40 45 gac gaa gat gac cac gag gac cat gac gat tcg gag aaa gtt aat gag 192 Asp Glu Asp Asp His Glu Asp His Asp Asp Ser Glu Lys Val Asn Glu 50 55 60 aca gac ggc gtt gaa gcc gag cgg ctg aaa gct ttt aat atg ttt gtc 240 Thr Asp Gly Val Glu Ala Glu Arg Leu Lys Ala Phe Asn Met Phe Val 65 70 75 80 agg ctg ttt gta gat gaa aac ttg gac cga atg gtc cca atc tct aag 288 Arg Leu Phe Val Asp Glu Asn Leu Asp Arg Met Val Pro Ile Ser Lys 85 90 95 cag ccc aaa gaa aag atc cag gct atc att gac tca tgc agg cga caa 336 Gln Pro Lys Glu Lys Ile Gln Ala Ile Ile Asp Ser Cys Arg Arg Gln 100 105 110 ttc cct gag tat caa gag cgt gcc aga aaa cgt ata cgt act tac ctc 384 Phe Pro Glu Tyr Gln Glu Arg Ala Arg Lys Arg Ile Arg Thr Tyr Leu 115 120 125 aag tcc tgc agg cgg atg aaa aga agt ggt ttt gag atg tct cga cct 432 Lys Ser Cys Arg Arg Met Lys Arg Ser Gly Phe Glu Met Ser Arg Pro 130 135 140 att cct tcc cac ctt act tca gca gtt gca gag agt atc ttg gct tca 480 Ile Pro Ser His Leu Thr Ser Ala Val Ala Glu Ser Ile Leu Ala Ser 145 150 155 160 gct tgt gag agt gag agt aga aat gcc gcc aag agg atg cgt ctg gag 528 Ala Cys Glu Ser Glu Ser Arg Asn Ala Ala Lys Arg Met Arg Leu Glu 165 170 175 aga cag cag gat gag tct gct cca gct gac aaa cag tgt aaa cca gag 576 Arg Gln Gln Asp Glu Ser Ala Pro Ala Asp Lys Gln Cys Lys Pro Glu 180 185 190 gcg acc cag gcc act tac tca aca tca gct gtt cca ggc tca cag gac 624 Ala Thr Gln Ala Thr Tyr Ser Thr Ser Ala Val Pro Gly Ser Gln Asp 195 200 205 gtg ctg tac atc aat gga aat ggg acc tat agt tac cat agt tac aga 672 Val Leu Tyr Ile Asn Gly Asn Gly Thr Tyr Ser Tyr His Ser Tyr Arg 210 215 220 ggg cta gga ggg ggt ctg cta aat ctg aat gat gct tcc agc agt gga 720 Gly Leu Gly Gly Gly Leu Leu Asn Leu Asn Asp Ala Ser Ser Ser Gly 225 230 235 240 ccc act gat ctc agc atg aag aga caa ttg gcg act agc tca gga tcc 768 Pro Thr Asp Leu Ser Met Lys Arg Gln Leu Ala Thr Ser Ser Gly Ser 245 250 255 tcc agc agc tca aac tcc aga ccc cag ctg agt cca act gaa atc aat 816 Ser Ser Ser Ser Asn Ser Arg Pro Gln Leu Ser Pro Thr Glu Ile Asn 260 265 270 gcc gtg aga cag ctt gtt gca gga tat cga gaa tca gct gca ttt tta 864 Ala Val Arg Gln Leu Val Ala Gly Tyr Arg Glu Ser Ala Ala Phe Leu 275 280 285 ttg cga tct gca gat gaa ctg gaa aat ctc att tta caa cag aac 909 Leu Arg Ser Ala Asp Glu Leu Glu Asn Leu Ile Leu Gln Gln Asn 290 295 300 tgagacagac gaccaccata ttcactgagg tctaaattgc agt 952 <210> 33 <211> 2774 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2230 <220> <221> CDS <222> (1)..(2598) <400> 33 ggg aag atg gcg gcg ccg ctc atc ccc ctc tcc cag cag att ccc act 48 Gly Lys Met Ala Ala Pro Leu Ile Pro Leu Ser Gln Gln Ile Pro Thr 1 5 10 15 gga aat tcg ttg tat gaa tct tat tac aag cag gtc gat ccg gca tac 96 Gly Asn Ser Leu Tyr Glu Ser Tyr Tyr Lys Gln Val Asp Pro Ala Tyr 20 25 30 aca ggg agg gtg ggg gcg agt gaa gct gcg ctt ttt cta aag aag tct 144 Thr Gly Arg Val Gly Ala Ser Glu Ala Ala Leu Phe Leu Lys Lys Ser 35 40 45 ggc ctc tcg gac att atc ctt ggg aag ata tgg gac ttg gcc gat cca 192 Gly Leu Ser Asp Ile Ile Leu Gly Lys Ile Trp Asp Leu Ala Asp Pro 50 55 60 gaa ggt aaa ggg ttc ttg gac aaa cag ggt ttc tat gtt gca ctg aga 240 Glu Gly Lys Gly Phe Leu Asp Lys Gln Gly Phe Tyr Val Ala Leu Arg 65 70 75 80 ctg gtg gcc tgt gca cag agt ggc cat gaa gtt acc ttg agc aat ctg 288 Leu Val Ala Cys Ala Gln Ser Gly His Glu Val Thr Leu Ser Asn Leu 85 90 95 aat ttg agc atg cca ccg cct aaa ttt cac gac acc agc agc cct ctg 336 Asn Leu Ser Met Pro Pro Pro Lys Phe His Asp Thr Ser Ser Pro Leu 100 105 110 atg gtc aca ccg ccc tct gca gag gcc cac tgg gct gtg agg gtg gaa 384 Met Val Thr Pro Pro Ser Ala Glu Ala His Trp Ala Val Arg Val Glu 115 120 125 gaa aag gcc aaa ttt gat ggg att ttt gaa agc ctc ttg ccc atc aat 432 Glu Lys Ala Lys Phe Asp Gly Ile Phe Glu Ser Leu Leu Pro Ile Asn 130 135 140 ggt ttg ctc tct gga gac aaa gtc aag cca gtc ctc atg aac tca aag 480 Gly Leu Leu Ser Gly Asp Lys Val Lys Pro Val Leu Met Asn Ser Lys 145 150 155 160 ctg cct ctt gat gtc ctg ggc agg gtc tgg gac ctc agt gac att gac 528 Leu Pro Leu Asp Val Leu Gly Arg Val Trp Asp Leu Ser Asp Ile Asp 165 170 175 aag gat ggg cac ttg gat cga gat gag ttc gct gtg gcc atg cac ttg 576 Lys Asp Gly His Leu Asp Arg Asp Glu Phe Ala Val Ala Met His Leu 180 185 190 gtg tac cga gcc ctg gag aag gag ccc gtg ccc tcc gcc ctg ccc ccg 624 Val Tyr Arg Ala Leu Glu Lys Glu Pro Val Pro Ser Ala Leu Pro Pro 195 200 205 tcc ctc atc cca ccc tcc aag aga aag aag act gtg ttc cct ggc gcc 672 Ser Leu Ile Pro Pro Ser Lys Arg Lys Lys Thr Val Phe Pro Gly Ala 210 215 220 gtc ccc gtc ctg cct gcc agc ccc cca cca aaa gac agc ctc cgc tcc 720 Val Pro Val Leu Pro Ala Ser Pro Pro Pro Lys Asp Ser Leu Arg Ser 225 230 235 240 acg ccg tcc cac ggc agc gtc agc agc ctc aac agc aca ggg agc ctg 768 Thr Pro Ser His Gly Ser Val Ser Ser Leu Asn Ser Thr Gly Ser Leu 245 250 255 tcc ccc aag cac agc ctc aag caa aca cag cca aca gtg aac tgg gtg 816 Ser Pro Lys His Ser Leu Lys Gln Thr Gln Pro Thr Val Asn Trp Val 260 265 270 gtg ccc gtg gca gac aag atg cga ttt gat gag ata ttc ctg aag acc 864 Val Pro Val Ala Asp Lys Met Arg Phe Asp Glu Ile Phe Leu Lys Thr 275 280 285 gac ctg gac ctg gat ggc tac gtg agt ggc cag gag gtg aag gag atc 912 Asp Leu Asp Leu Asp Gly Tyr Val Ser Gly Gln Glu Val Lys Glu Ile 290 295 300 ttc atg cac tcg ggc ctc acc cag aac ctt cta gca cac ata tgg gcc 960 Phe Met His Ser Gly Leu Thr Gln Asn Leu Leu Ala His Ile Trp Ala 305 310 315 320 ctg gcc gat acg agg caa acg ggg aag tta agc aaa gac caa ttc gcg 1008 Leu Ala Asp Thr Arg Gln Thr Gly Lys Leu Ser Lys Asp Gln Phe Ala 325 330 335 tta gct atg tat ttc att cag cag aag gtc agt aaa ggc atc gac cct 1056 Leu Ala Met Tyr Phe Ile Gln Gln Lys Val Ser Lys Gly Ile Asp Pro 340 345 350 cct caa gtc ctc tcg ccg gac atg gtc ccg cct tcg gag aga ggc acg 1104 Pro Gln Val Leu Ser Pro Asp Met Val Pro Pro Ser Glu Arg Gly Thr 355 360 365 ccc ggc ccg gac agt tca ggc tct ctc ggc tcc ggg gag ttt act ggc 1152 Pro Gly Pro Asp Ser Ser Gly Ser Leu Gly Ser Gly Glu Phe Thr Gly 370 375 380 gtg aag gag ctt gat gac atc agt caa gag att gcc cag tta caa aga 1200 Val Lys Glu Leu Asp Asp Ile Ser Gln Glu Ile Ala Gln Leu Gln Arg 385 390 395 400 gag aaa tat tca ctg gaa caa gac att cga gaa aag gaa gag gca atc 1248 Glu Lys Tyr Ser Leu Glu Gln Asp Ile Arg Glu Lys Glu Glu Ala Ile 405 410 415 aga cag aaa acc agc gag gtg cag gaa tta caa aat gac cta gac cgg 1296 Arg Gln Lys Thr Ser Glu Val Gln Glu Leu Gln Asn Asp Leu Asp Arg 420 425 430 gaa aca agc agt ttg cag gag ctc gag gct cag aaa cag gat gct caa 1344 Glu Thr Ser Ser Leu Gln Glu Leu Glu Ala Gln Lys Gln Asp Ala Gln 435 440 445 gac cgc ctg gac gag atg gac cag cag aag gcc aag ctc cga gac atg 1392 Asp Arg Leu Asp Glu Met Asp Gln Gln Lys Ala Lys Leu Arg Asp Met 450 455 460 ctg agc gac gtc cgg cag aag tgc cag gat gag act cag atg atc tca 1440 Leu Ser Asp Val Arg Gln Lys Cys Gln Asp Glu Thr Gln Met Ile Ser 465 470 475 480 tca ctg aaa acg caa atc caa tct cag gaa tct gac tta aag tcc cag 1488 Ser Leu Lys Thr Gln Ile Gln Ser Gln Glu Ser Asp Leu Lys Ser Gln 485 490 495 gaa gac gat ctg aac cga gcc aag tcg gag ctg aac cga ttg cag cag 1536 Glu Asp Asp Leu Asn Arg Ala Lys Ser Glu Leu Asn Arg Leu Gln Gln 500 505 510 gag gaa acc cag ctg gag cag agc att cag gct ggg cga gtc cag ctg 1584 Glu Glu Thr Gln Leu Glu Gln Ser Ile Gln Ala Gly Arg Val Gln Leu 515 520 525 gaa acc atc atc aag tcc ctg aag tca acg caa gac gaa atc aac cag 1632 Glu Thr Ile Ile Lys Ser Leu Lys Ser Thr Gln Asp Glu Ile Asn Gln 530 535 540 gca agg agc aaa ctt tcc cag ctg cat gaa agc cgc cag gag gcc cac 1680 Ala Arg Ser Lys Leu Ser Gln Leu His Glu Ser Arg Gln Glu Ala His 545 550 555 560 agg agc ctg gag cag tat gac cag gtg ctc gat gga gcc cat ggt gcc 1728 Arg Ser Leu Glu Gln Tyr Asp Gln Val Leu Asp Gly Ala His Gly Ala 565 570 575 agc ctg acc gac ctg gcc aac ctg agc gaa ggc gtc tcc ctg gca gag 1776 Ser Leu Thr Asp Leu Ala Asn Leu Ser Glu Gly Val Ser Leu Ala Glu 580 585 590 agg ggc agt ttt gga gcc atg gat gat cct ttc aaa aat aaa gcc ttg 1824 Arg Gly Ser Phe Gly Ala Met Asp Asp Pro Phe Lys Asn Lys Ala Leu 595 600 605 tta ttt agc aac aac acg caa gag ttg cat ccg gat cct ttc cag aca 1872 Leu Phe Ser Asn Asn Thr Gln Glu Leu His Pro Asp Pro Phe Gln Thr 610 615 620 gaa gac ccc ttc aaa tct gac cca ttt aaa gga gct gac ccc ttc aaa 1920 Glu Asp Pro Phe Lys Ser Asp Pro Phe Lys Gly Ala Asp Pro Phe Lys 625 630 635 640 ggc gac ccg ttc cag aat gac ccc ttt gca gaa cag cag aca act tca 1968 Gly Asp Pro Phe Gln Asn Asp Pro Phe Ala Glu Gln Gln Thr Thr Ser 645 650 655 aca gat cca ttt gga ggg gac cct ttc aaa gaa agt gac cca ttc cgt 2016 Thr Asp Pro Phe Gly Gly Asp Pro Phe Lys Glu Ser Asp Pro Phe Arg 660 665 670 ggc tct gcc act gac gac ttc ttc aag aaa cag aca aag aat gac cca 2064 Gly Ser Ala Thr Asp Asp Phe Phe Lys Lys Gln Thr Lys Asn Asp Pro 675 680 685 ttt acc tcg gat cca ttc acg aaa aac cct tcc tta cct tcg aag ctc 2112 Phe Thr Ser Asp Pro Phe Thr Lys Asn Pro Ser Leu Pro Ser Lys Leu 690 695 700 gac ccc ttt gaa tcc agt gat ccc ttt tca tcc tcc agt gtc tcc tca 2160 Asp Pro Phe Glu Ser Ser Asp Pro Phe Ser Ser Ser Ser Val Ser Ser 705 710 715 720 aaa gga tca gat ccc ttt gga acc tta gat ccc ttc gga agt ggg tcc 2208 Lys Gly Ser Asp Pro Phe Gly Thr Leu Asp Pro Phe Gly Ser Gly Ser 725 730 735 ttc aat agt gct gaa ggc ttt gcc gac ttc agc cag atg tcc aag ccc 2256 Phe Asn Ser Ala Glu Gly Phe Ala Asp Phe Ser Gln Met Ser Lys Pro 740 745 750 cca cct tct ggg cct ttc acc tcc tcc ttg gga ggg gca gga ttc tca 2304 Pro Pro Ser Gly Pro Phe Thr Ser Ser Leu Gly Gly Ala Gly Phe Ser 755 760 765 gat gac ccc ttt aaa agt aaa cag gac act cct gct ctg cct ccg aag 2352 Asp Asp Pro Phe Lys Ser Lys Gln Asp Thr Pro Ala Leu Pro Pro Lys 770 775 780 aaa cct gct cct cca cgg cct aaa ccg ccc agc ggt aaa agt aca cct 2400 Lys Pro Ala Pro Pro Arg Pro Lys Pro Pro Ser Gly Lys Ser Thr Pro 785 790 795 800 gta agc cag ctt ggt tcc gca gac ttt ccc gag gcc ccc gat cca ttc 2448 Val Ser Gln Leu Gly Ser Ala Asp Phe Pro Glu Ala Pro Asp Pro Phe 805 810 815 cag cca ctc ggg gct gac agc ggc gac ccg ttc caa agt aaa aag ggg 2496 Gln Pro Leu Gly Ala Asp Ser Gly Asp Pro Phe Gln Ser Lys Lys Gly 820 825 830 ttt ggg gac ccg ttt agt gga aaa gac cca ttt gtc ccc tcc tct gca 2544 Phe Gly Asp Pro Phe Ser Gly Lys Asp Pro Phe Val Pro Ser Ser Ala 835 840 845 gct aaa cct tct aag gcc tct gcc tcg ggc ttt gca gac ttc acc tct 2592 Ala Lys Pro Ser Lys Ala Ser Ala Ser Gly Phe Ala Asp Phe Thr Ser 850 855 860 gta agt tgagtcctcc gcctccgggc caccccactc ccttccgctt gcagcttccc 2648 Val Ser 865 tgggattttt gtctcctttt aaaggcaaac ctcccagctt ctttagcctc ttggtacctc 2708 acactctctg tccctcgcgt tatttattct acactgccac ttctgtaaga aaaacagttt 2768 ctcaat 2774 <210> 34 <211> 973 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2063 <220> <221> CDS <222> (1)..(807) <400> 34 gac gcg tgg gcg gtg aag gag gag tgc cgc ctg ctc aat gcc cct cca 48 Asp Ala Trp Ala Val Lys Glu Glu Cys Arg Leu Leu Asn Ala Pro Pro 1 5 10 15 gtg cct cct cgg ggt ggc aat ggc agc ggc cgg ctc tcc agc agc ccc 96 Val Pro Pro Arg Gly Gly Asn Gly Ser Gly Arg Leu Ser Ser Ser Pro 20 25 30 ccg gtt ccc cct cgc ttc ccc aag ctg cag ccg gta cat tcc ccc agc 144 Pro Val Pro Pro Arg Phe Pro Lys Leu Gln Pro Val His Ser Pro Ser 35 40 45 tcc agc ctc tcc tac tac tcc tct ggc ctc cag gat ggg gcg ggt tcc 192 Ser Ser Leu Ser Tyr Tyr Ser Ser Gly Leu Gln Asp Gly Ala Gly Ser 50 55 60 cgc agt ggc agt ggc tcc cca tcg ccg gac acc tac tcc ctc tat tgc 240 Arg Ser Gly Ser Gly Ser Pro Ser Pro Asp Thr Tyr Ser Leu Tyr Cys 65 70 75 80 tac cca tgc acc tgg gga gac tgc aag gaa cca gtc ctg gag ccc ttc 288 Tyr Pro Cys Thr Trp Gly Asp Cys Lys Glu Pro Val Leu Glu Pro Phe 85 90 95 gat ccc ttt gag ctg ggg cag ggc agt tct cca gag cct gag ctg ctg 336 Asp Pro Phe Glu Leu Gly Gln Gly Ser Ser Pro Glu Pro Glu Leu Leu 100 105 110 cgt tct cag gag ccc aga gca gtg ggg aca cct ggg cct gga ccc cgc 384 Arg Ser Gln Glu Pro Arg Ala Val Gly Thr Pro Gly Pro Gly Pro Arg 115 120 125 ctt tca cca ctt ggc ccc tcc aag gcc ttt gag cct gaa ggt ttg gtg 432 Leu Ser Pro Leu Gly Pro Ser Lys Ala Phe Glu Pro Glu Gly Leu Val 130 135 140 ctg cac cag gtc ccc acc cca ctg tca cca gct gct ctg cag gga ccc 480 Leu His Gln Val Pro Thr Pro Leu Ser Pro Ala Ala Leu Gln Gly Pro 145 150 155 160 gag gcg gga gga gca ctt ttt cta acc caa ggg cgc ctg gaa ggg cct 528 Glu Ala Gly Gly Ala Leu Phe Leu Thr Gln Gly Arg Leu Glu Gly Pro 165 170 175 cct gcc agt ccc cgg gat gga gcc aca ggc ttt gga gtc cga gat gcc 576 Pro Ala Ser Pro Arg Asp Gly Ala Thr Gly Phe Gly Val Arg Asp Ala 180 185 190 tcc tcc tgg cag ccc cct gct gac ctg tct gca ctc tcc ctg gag gag 624 Ser Ser Trp Gln Pro Pro Ala Asp Leu Ser Ala Leu Ser Leu Glu Glu 195 200 205 gtc tct cgc agt ctg cgt ttc atc ggg ctc tca gag gat gtg gtg agc 672 Val Ser Arg Ser Leu Arg Phe Ile Gly Leu Ser Glu Asp Val Val Ser 210 215 220 ttc ttt gcc cga gaa cgc atc gat ggt agc atc ttt gtg cag ctc agt 720 Phe Phe Ala Arg Glu Arg Ile Asp Gly Ser Ile Phe Val Gln Leu Ser 225 230 235 240 gag gac atc ctg gca gat gac ttc cac ctc acc aag ctg cag gtc aag 768 Glu Asp Ile Leu Ala Asp Asp Phe His Leu Thr Lys Leu Gln Val Lys 245 250 255 aag atc atg cag ttc atc aaa ggc tgg agg ccc aag atc tgaactgccc 817 Lys Ile Met Gln Phe Ile Lys Gly Trp Arg Pro Lys Ile 260 265 agctggagct gcacagctgg aatgctggta tgggggcccc aggtacagca ctccggagga 877 gcaggtgctg cctgcaagaa ggatctatgt cgagactgag gctgctcagc agccactggg 937 tggatccagg ggagatgcat gtggaaatgt ggtcct 973 <210> 35 <211> 536 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2411 <220> <221> CDS <222> (1)..(162) <400> 35 gag gag gaa gag gaa gaa agt gaa aat aaa agt atg gat acg gat ccc 48 Glu Glu Glu Glu Glu Glu Ser Glu Asn Lys Ser Met Asp Thr Asp Pro 1 5 10 15 gaa acg ata cga gat gaa gaa gag act gga gat cac tcc atg gac gat 96 Glu Thr Ile Arg Asp Glu Glu Glu Thr Gly Asp His Ser Met Asp Asp 20 25 30 agt tcg gag gat ggg aaa atg gaa acc aaa tca gac cac gag gaa gac 144 Ser Ser Glu Asp Gly Lys Met Glu Thr Lys Ser Asp His Glu Glu Asp 35 40 45 aat atg gaa gat ggc atg taataaacta ctgcatttta agcttcctat 192 Asn Met Glu Asp Gly Met 50 ttttttttcc agtagtattg ttacctgctt gaaaacactg ctgtgttaag ctgttcatgc 252 acgtgcctga cgcttccagg aagctgtaga gagggacaga aggggcggtt cagccaagac 312 agatgtagac ggagttggag ctgggtattg ttaaaaactg cattatgcaa aaattttgta 372 cagtgttaag gcctaaaaac tgtgtggttc agagactaat tcctgtgttt aatagcattt 432 atactttaag cacaactaga aaattgtaag aattgcactc tacttatgta tcactacaaa 492 ctttaaaaaa ctatgtctaa tttatattaa tacattttaa aaag 536 <210> 36 <211> 1065 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2436 <220> <221> CDS <222> (1)..(672) <400> 36 tcc tcc gat ccc gct gac ttc gaa cca atc cca tct ttt tct ggg ttt 48 Ser Ser Asp Pro Ala Asp Phe Glu Pro Ile Pro Ser Phe Ser Gly Phe 1 5 10 15 ccg tta gat tct ccc aaa acc ttg gtg ctt gac ttt gag aca gag ggt 96 Pro Leu Asp Ser Pro Lys Thr Leu Val Leu Asp Phe Glu Thr Glu Gly 20 25 30 gaa cga aac tca cct aat ccc agg agt gtt agg atc cct tct cct aac 144 Glu Arg Asn Ser Pro Asn Pro Arg Ser Val Arg Ile Pro Ser Pro Asn 35 40 45 att ttg aaa act gga ctt aca gaa aat gtt gac cgt ggc ttg ggg ggc 192 Ile Leu Lys Thr Gly Leu Thr Glu Asn Val Asp Arg Gly Leu Gly Gly 50 55 60 cta gag gga aca cac cag gcc ctt gac ctg tta gca gga gga atg atg 240 Leu Glu Gly Thr His Gln Ala Leu Asp Leu Leu Ala Gly Gly Met Met 65 70 75 80 cct gag gaa gta aaa gaa tct tcc caa tta gac aaa caa gag tca ctc 288 Pro Glu Glu Val Lys Glu Ser Ser Gln Leu Asp Lys Gln Glu Ser Leu 85 90 95 gga ttg gaa tta aaa aca att aat tct gca ggc ctt ggg cca tct cct 336 Gly Leu Glu Leu Lys Thr Ile Asn Ser Ala Gly Leu Gly Pro Ser Pro 100 105 110 tgc ctt cca gac ctt gat gac ttt gtc aca cgg acc tct gga gtt caa 384 Cys Leu Pro Asp Leu Asp Asp Phe Val Thr Arg Thr Ser Gly Val Gln 115 120 125 aaa gat aaa ctg tgt tct cca ctc tct gag cca ggt gac cct tct aaa 432 Lys Asp Lys Leu Cys Ser Pro Leu Ser Glu Pro Gly Asp Pro Ser Lys 130 135 140 tgt agt tcc ctg gag ttg ggg cca tta cag cta gaa ata tcg aat gca 480 Cys Ser Ser Leu Glu Leu Gly Pro Leu Gln Leu Glu Ile Ser Asn Ala 145 150 155 160 tcc acc aca gag gtg gca att ctg caa gta gat gat gac agt ggc gac 528 Ser Thr Thr Glu Val Ala Ile Leu Gln Val Asp Asp Asp Ser Gly Asp 165 170 175 cct ctg aat ttg gtt aaa gct cca gtg tca agg tcc cct cca agg gag 576 Pro Leu Asn Leu Val Lys Ala Pro Val Ser Arg Ser Pro Pro Arg Glu 180 185 190 cag gta att gaa gac aat atg gtc cct cag gga atg cct gaa cag gaa 624 Gln Val Ile Glu Asp Asn Met Val Pro Gln Gly Met Pro Glu Gln Glu 195 200 205 act aca gtt ggt gcc atc cag gac cac aca gaa tcc agt gtt cac aac 672 Thr Thr Val Gly Ala Ile Gln Asp His Thr Glu Ser Ser Val His Asn 210 215 220 taagaataaa tacctagagc tacttgtgag tgaatcattg gcttctagaa atcagatgcc 732 cagatgatcc aagactagtt tgttatctca tctggaacct acaccaagag atgcagtcag 792 catcttaaag taaatgttca tggaagctaa aaaatttacc tcctttcctt ttccctttcc 852 ccttaaaaca ccagacaaat cgataacaac ttatatataa tatatatata tctgtaaaaa 912 tgtattttgt taattttttt ggccaattta ttttctccca ctttgtcatt aaaatcacat 972 gtctaactgg ctcaccacat agtgtgcttt gattgtattt tggctttgac tctgccattt 1032 ctgaaatgtg ggggaggatg gaggcagctg cct 1065 <210> 37 <211> 910 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2122 <220> <221> CDS <222> (1)..(672) <400> 37 gag cgc agc ctg gca gag atg gag tcc tcg cac cag cag gtg atg gag 48 Glu Arg Ser Leu Ala Glu Met Glu Ser Ser His Gln Gln Val Met Glu 1 5 10 15 gag ctg cag cgg cac cac gag cgg gag ctg cag cgc ctg cag cag gag 96 Glu Leu Gln Arg His His Glu Arg Glu Leu Gln Arg Leu Gln Gln Glu 20 25 30 aag gag tgg ctc ctg gct gag gag acg gca gcc acg gcc tca gcc att 144 Lys Glu Trp Leu Leu Ala Glu Glu Thr Ala Ala Thr Ala Ser Ala Ile 35 40 45 gaa gcc atg aag aag gcc tac cag gaa gag ctg agc cga gag ctg agc 192 Glu Ala Met Lys Lys Ala Tyr Gln Glu Glu Leu Ser Arg Glu Leu Ser 50 55 60 aaa aca cgg agt ctc cag cag ggc ccg gat ggc ctc cgg aag cag cac 240 Lys Thr Arg Ser Leu Gln Gln Gly Pro Asp Gly Leu Arg Lys Gln His 65 70 75 80 cag tca gat gtg gag gca ctg aag cga gag ctg cag gtg cta tcg gag 288 Gln Ser Asp Val Glu Ala Leu Lys Arg Glu Leu Gln Val Leu Ser Glu 85 90 95 cag tac tcg cag aag tgc ctg gag att ggg gca ctc atg cgg cag gct 336 Gln Tyr Ser Gln Lys Cys Leu Glu Ile Gly Ala Leu Met Arg Gln Ala 100 105 110 gag gag cgc gag cac acg ctg cgc cgc tgc cag cag gag ggc cag gag 384 Glu Glu Arg Glu His Thr Leu Arg Arg Cys Gln Gln Glu Gly Gln Glu 115 120 125 ctg ctg cgc cac aac cag gag ctg cat ggg ccg cct gtc aga gga gat 432 Leu Leu Arg His Asn Gln Glu Leu His Gly Pro Pro Val Arg Gly Asp 130 135 140 aga cca gct gcg cgg ctt cat tgc ctc gca ggg cat ggg caa tgg ctg 480 Arg Pro Ala Ala Arg Leu His Cys Leu Ala Gly His Gly Gln Trp Leu 145 150 155 160 cgg gcg cag caa cga gcg gag ttc ctg cga gct aga ggt gct gct tcg 528 Arg Ala Gln Gln Arg Ala Glu Phe Leu Arg Ala Arg Gly Ala Ala Ser 165 170 175 cgt aaa aga aaa cga act cca gta cct aaa gaa gga ggt gca gtg cct 576 Arg Lys Arg Lys Arg Thr Pro Val Pro Lys Glu Gly Gly Ala Val Pro 180 185 190 ccg gga cga gct cca gat gat gca gaa gga caa gcg ctt cac ctc ggg 624 Pro Gly Arg Ala Pro Asp Asp Ala Glu Gly Gln Ala Leu His Leu Gly 195 200 205 aaa gta cca gga cgt cta tgt gga gct gag cca cat caa gac acg gtc 672 Lys Val Pro Gly Arg Leu Cys Gly Ala Glu Pro His Gln Asp Thr Val 210 215 220 tgagcgggag atcgagcagc tgaaggagca cctgcgtctt gccatggccg ccctccagga 732 gaaggagtcg atgcgcaaca gcctggctga gtagaggtcc cgcccagctg cagaccctcc 792 aggctggagg accagccgcc ctccttccct cctggatgga agtaaaaagc caagctttct 852 ccccaccctc tgtgggccac acgtgcactt gcacccacca cacacacaca cacacaca 910 <210> 38 <211> 1332 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2157 <220> <221> CDS <222> (1)..(1224) <400> 38 aga aac cca gaa atc agt cac ctg ctc aac aac cca gac ata atg agg 48 Arg Asn Pro Glu Ile Ser His Leu Leu Asn Asn Pro Asp Ile Met Arg 1 5 10 15 cag aca ctc gaa att gcc agg aat cca gcc atg atg caa gag atg atg 96 Gln Thr Leu Glu Ile Ala Arg Asn Pro Ala Met Met Gln Glu Met Met 20 25 30 aga aat caa gac ctg gct ctt agc aat cta gaa agc atc cca ggt ggc 144 Arg Asn Gln Asp Leu Ala Leu Ser Asn Leu Glu Ser Ile Pro Gly Gly 35 40 45 tat aat gct tta cgg cgc atg tac act gac att caa gag ccg atg ctg 192 Tyr Asn Ala Leu Arg Arg Met Tyr Thr Asp Ile Gln Glu Pro Met Leu 50 55 60 aat gcc gca caa gag cag ttt ggg ggt aat cca ttt gcc tcc gtg ggg 240 Asn Ala Ala Gln Glu Gln Phe Gly Gly Asn Pro Phe Ala Ser Val Gly 65 70 75 80 agt agt tcc tcc tct ggg gaa ggt acg cag cct tcc cgc aca gaa aat 288 Ser Ser Ser Ser Ser Gly Glu Gly Thr Gln Pro Ser Arg Thr Glu Asn 85 90 95 cgc gat cca cta ccc aat cca tgg gca cca ccg cca gct acc cag agt 336 Arg Asp Pro Leu Pro Asn Pro Trp Ala Pro Pro Pro Ala Thr Gln Ser 100 105 110 tct gca act acc agc acg acc aca agc act ggt agt ggg tct ggc aat 384 Ser Ala Thr Thr Ser Thr Thr Thr Ser Thr Gly Ser Gly Ser Gly Asn 115 120 125 agt tcc agc aat gct act ggg aac acc gtt gct gcc gct aat tat gtc 432 Ser Ser Ser Asn Ala Thr Gly Asn Thr Val Ala Ala Ala Asn Tyr Val 130 135 140 gcc agc atc ttt agt acc cca ggc atg cag agc ctg ctg caa cag ata 480 Ala Ser Ile Phe Ser Thr Pro Gly Met Gln Ser Leu Leu Gln Gln Ile 145 150 155 160 act gaa aac ccc cag ctg att cag aat atg ctg tcg gcg ccc tac atg 528 Thr Glu Asn Pro Gln Leu Ile Gln Asn Met Leu Ser Ala Pro Tyr Met 165 170 175 aga agc atg atg cag tcg ctg agc cag aat cca gat ttg gct gca cag 576 Arg Ser Met Met Gln Ser Leu Ser Gln Asn Pro Asp Leu Ala Ala Gln 180 185 190 atg atg ctg aat agc ccg ctg ttt act gca aat cct cag ctg cag gag 624 Met Met Leu Asn Ser Pro Leu Phe Thr Ala Asn Pro Gln Leu Gln Glu 195 200 205 cag atg cgg cca cag ctc cca gcc ttc ctg cag cag atg cag aat cca 672 Gln Met Arg Pro Gln Leu Pro Ala Phe Leu Gln Gln Met Gln Asn Pro 210 215 220 gac aca cta tca gcc atg tca aac cca aga gca atg cag gct tta atg 720 Asp Thr Leu Ser Ala Met Ser Asn Pro Arg Ala Met Gln Ala Leu Met 225 230 235 240 cag atc cag cag ggg cta cag aca tta gcc act gaa gca cct ggc ctg 768 Gln Ile Gln Gln Gly Leu Gln Thr Leu Ala Thr Glu Ala Pro Gly Leu 245 250 255 att ccg agc ttc act cca ggt gtg ggg gtg ggg gtg ctg gga acc gct 816 Ile Pro Ser Phe Thr Pro Gly Val Gly Val Gly Val Leu Gly Thr Ala 260 265 270 ata ggc cct gta ggc cca gtc acc ccc ata ggc ccc ata ggc cct ata 864 Ile Gly Pro Val Gly Pro Val Thr Pro Ile Gly Pro Ile Gly Pro Ile 275 280 285 gtc cct ttt acc ccc ata ggc ccc att ggg ccc ata gga ccc act ggc 912 Val Pro Phe Thr Pro Ile Gly Pro Ile Gly Pro Ile Gly Pro Thr Gly 290 295 300 cct gca gcc ccc cct ggc tcc acc ggc tct ggt ggc ccc acg ggg cct 960 Pro Ala Ala Pro Pro Gly Ser Thr Gly Ser Gly Gly Pro Thr Gly Pro 305 310 315 320 act gtg tcc agc gct gca cct aga gaa acc acg agt cct aca tca gaa 1008 Thr Val Ser Ser Ala Ala Pro Arg Glu Thr Thr Ser Pro Thr Ser Glu 325 330 335 tct gga ccc aac cag cag ttc att cag caa atg gtg cag gcc ctg gct 1056 Ser Gly Pro Asn Gln Gln Phe Ile Gln Gln Met Val Gln Ala Leu Ala 340 345 350 gga gca aat gct cca cag ctg ccg aat cca gaa gtc aga ttt cag caa 1104 Gly Ala Asn Ala Pro Gln Leu Pro Asn Pro Glu Val Arg Phe Gln Gln 355 360 365 caa ctg gaa cag ctc aac gca atg ggg ttc tta aac cgt gaa gca aac 1152 Gln Leu Glu Gln Leu Asn Ala Met Gly Phe Leu Asn Arg Glu Ala Asn 370 375 380 ttg cag gcc cta ata gca aca gga ggc gac atc aat gca gcc att gaa 1200 Leu Gln Ala Leu Ile Ala Thr Gly Gly Asp Ile Asn Ala Ala Ile Glu 385 390 395 400 agg ctg ctg ggc tcc cag cca tcg taatcacatt tctgtacctg gaaaaaaaat 1254 Arg Leu Leu Gly Ser Gln Pro Ser 405 gtatcttatt tttgataatg gctcttaaat ctttaaacac acacacaaaa tcgtctttac 1314 tttcattttg attctttt 1332 <210> 39 <211> 1605 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2216 <220> <221> CDS <222> (1)..(1458) <400> 39 aat gcc gag agg atg gag agc atc ctg cag gca ctg gag gat att cag 48 Asn Ala Glu Arg Met Glu Ser Ile Leu Gln Ala Leu Glu Asp Ile Gln 1 5 10 15 ctg gat ctg gag gca gtg aac atc aag gca ggc aaa gcc ttc ctg cgt 96 Leu Asp Leu Glu Ala Val Asn Ile Lys Ala Gly Lys Ala Phe Leu Arg 20 25 30 ctc aag cgc aag ttc atc cag atg cga aga ccc ttc ctg gag cgc aga 144 Leu Lys Arg Lys Phe Ile Gln Met Arg Arg Pro Phe Leu Glu Arg Arg 35 40 45 gac ctc atc atc cag cat atc cca ggc ttc tgg gtc aaa gca ttc ctc 192 Asp Leu Ile Ile Gln His Ile Pro Gly Phe Trp Val Lys Ala Phe Leu 50 55 60 aac cac ccc aga att tca att ttg atc aac cga cgt gat gaa gac att 240 Asn His Pro Arg Ile Ser Ile Leu Ile Asn Arg Arg Asp Glu Asp Ile 65 70 75 80 ttc cgc tac ttg acc aat ctg cag gta cag gat ctc aga cat atc tcc 288 Phe Arg Tyr Leu Thr Asn Leu Gln Val Gln Asp Leu Arg His Ile Ser 85 90 95 atg ggc tac aaa atg aag ctg tac ttc cag act aac ccc tac ttc aca 336 Met Gly Tyr Lys Met Lys Leu Tyr Phe Gln Thr Asn Pro Tyr Phe Thr 100 105 110 aac atg gtg att gtc aag gag ttc cag cgc aac cgc tca ggc cgg ctg 384 Asn Met Val Ile Val Lys Glu Phe Gln Arg Asn Arg Ser Gly Arg Leu 115 120 125 gtg tct cac tca acc cca atc cgc tgg cac cgg ggc cag gaa ccc cag 432 Val Ser His Ser Thr Pro Ile Arg Trp His Arg Gly Gln Glu Pro Gln 130 135 140 gcc cgt cgt cac ggg aac cag gat gcg agc cac agc ttt ttc agc tgg 480 Ala Arg Arg His Gly Asn Gln Asp Ala Ser His Ser Phe Phe Ser Trp 145 150 155 160 ttc tca aac cat agc ctc cca gag gct gac agg att gct gag att atc 528 Phe Ser Asn His Ser Leu Pro Glu Ala Asp Arg Ile Ala Glu Ile Ile 165 170 175 aag aat gat ctg tgg gtt aac cct cta cgc tac tac ctg aga gaa agg 576 Lys Asn Asp Leu Trp Val Asn Pro Leu Arg Tyr Tyr Leu Arg Glu Arg 180 185 190 ggc tcc agg ata aag aga aag aag caa gaa atg aag aaa cgt aaa acc 624 Gly Ser Arg Ile Lys Arg Lys Lys Gln Glu Met Lys Lys Arg Lys Thr 195 200 205 agg ggc aga tgt gag gtg gtg atc atg gaa gac gcc cct gac tat tat 672 Arg Gly Arg Cys Glu Val Val Ile Met Glu Asp Ala Pro Asp Tyr Tyr 210 215 220 gca gtg gaa gac att ttc agc gag atc tca gac att gat gag aca att 720 Ala Val Glu Asp Ile Phe Ser Glu Ile Ser Asp Ile Asp Glu Thr Ile 225 230 235 240 cat gac atc aag atc tct gac ttc atg gag acc acc gac tac ttc gag 768 His Asp Ile Lys Ile Ser Asp Phe Met Glu Thr Thr Asp Tyr Phe Glu 245 250 255 acc act gac aat gag ata act gac atc aat gag aac atc tgc gac agc 816 Thr Thr Asp Asn Glu Ile Thr Asp Ile Asn Glu Asn Ile Cys Asp Ser 260 265 270 gag aat cct gac cac aat gag gtc ccc aac aac gag acc act gat aac 864 Glu Asn Pro Asp His Asn Glu Val Pro Asn Asn Glu Thr Thr Asp Asn 275 280 285 aac gag agt gct gat gac cac gaa acc act gac aac aat gag agt gca 912 Asn Glu Ser Ala Asp Asp His Glu Thr Thr Asp Asn Asn Glu Ser Ala 290 295 300 gat gac aac aac gag aat cct gaa gac aat aac aag aac act gat gac 960 Asp Asp Asn Asn Glu Asn Pro Glu Asp Asn Asn Lys Asn Thr Asp Asp 305 310 315 320 aac gaa gag aac cct aac aac aac gag aac act tac ggc aac aac ttc 1008 Asn Glu Glu Asn Pro Asn Asn Asn Glu Asn Thr Tyr Gly Asn Asn Phe 325 330 335 ttc aaa ggt ggc ttc tgg ggc agc cat ggc aac aac cag gac agc agc 1056 Phe Lys Gly Gly Phe Trp Gly Ser His Gly Asn Asn Gln Asp Ser Ser 340 345 350 gac agt gac aat gaa gca gat gag gcc agt gat gat gaa gat aat gat 1104 Asp Ser Asp Asn Glu Ala Asp Glu Ala Ser Asp Asp Glu Asp Asn Asp 355 360 365 ggc aac gaa ggt gac aat gag ggc agt gat gat gat ggc aat gaa ggt 1152 Gly Asn Glu Gly Asp Asn Glu Gly Ser Asp Asp Asp Gly Asn Glu Gly 370 375 380 gac aat gaa ggc agc gat gat gac gac aga gac att gag tac tat gag 1200 Asp Asn Glu Gly Ser Asp Asp Asp Asp Arg Asp Ile Glu Tyr Tyr Glu 385 390 395 400 aaa ggt att gaa gac ttt gac agg gat cag gct gac tac gag gac gtg 1248 Lys Gly Ile Glu Asp Phe Asp Arg Asp Gln Ala Asp Tyr Glu Asp Val 405 410 415 ata gag atc atc tca gac gaa tca gtg gaa gaa gag ggc att gag gaa 1296 Ile Glu Ile Ile Ser Asp Glu Ser Val Glu Glu Glu Gly Ile Glu Glu 420 425 430 ggc atc cag caa gat gag gac atc tat gag gaa gga aac tat gag gag 1344 Gly Ile Gln Gln Asp Glu Asp Ile Tyr Glu Glu Gly Asn Tyr Glu Glu 435 440 445 gaa gga agt gaa gat gtc tgg gaa gaa ggg gaa gat tcg gac gac tct 1392 Glu Gly Ser Glu Asp Val Trp Glu Glu Gly Glu Asp Ser Asp Asp Ser 450 455 460 gac cta gag gat gtg ctt cag gtc cca aac ggt tgg gcc aat ccg ggg 1440 Asp Leu Glu Asp Val Leu Gln Val Pro Asn Gly Trp Ala Asn Pro Gly 465 470 475 480 aag agg ggg aaa acc gga taagggtttt ccccttttgg ggatcacctc 1488 Lys Arg Gly Lys Thr Gly 485 tctgtatccc ccacccacta tcccatttgc cctcctcctc agctagggcc acgcggaccc 1548 acattgcact tctggggggt gaccgacttc gtacacgggt ttaaagttta ttttttt 1605 <210> 40 <211> 532 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2025 <220> <221> CDS <222> (1)..(201) <400> 40 ata gca gag aca ggg gtt agt ggc ggt gtg gac cgg agg gag gtt cag 48 Ile Ala Glu Thr Gly Val Ser Gly Gly Val Asp Arg Arg Glu Val Gln 1 5 10 15 cgc ctt cgc agg cgg aac cag cag ttg gag gaa gag aac aat ctc ttg 96 Arg Leu Arg Arg Arg Asn Gln Gln Leu Glu Glu Glu Asn Asn Leu Leu 20 25 30 cgg ctg aaa gtg gac atc tta tta gac atg ctt tca gag tcc act gcc 144 Arg Leu Lys Val Asp Ile Leu Leu Asp Met Leu Ser Glu Ser Thr Ala 35 40 45 gaa tcc cac tta atg gag aag gaa ctg gat gaa ctg agg atc agc cgg 192 Glu Ser His Leu Met Glu Lys Glu Leu Asp Glu Leu Arg Ile Ser Arg 50 55 60 aag aga aaa tgaagacccc agagacattt attggggagt aggatgtggc 241 Lys Arg Lys 65 tgagtgcttt ttttttggcc agactagcgg attcagtcct ggaagagagt atcatataat 301 gagacccaca ggcactggca cccttgggtt ggcaatagaa ggtgacatgg aatggagaaa 361 accaagattc cagatgggga tagtaactag aaggtgcttc agatgcactg cctgcgggtg 421 ccagtctgaa aaccagaccg cacagaggcc tggggctgct gatgagcttt tcggtgctct 481 cacacacaag ctcgcaaaca cacatgtccc agaatagctc tgtgggtgtg t 532 <210> 41 <211> 519 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2281 <220> <221> CDS <222> (1)..(324) <400> 41 gag ggc ccc ttg gac agc ccc tcg gag ggc cac agt tat cag aac agc 48 Glu Gly Pro Leu Asp Ser Pro Ser Glu Gly His Ser Tyr Gln Asn Ser 1 5 10 15 ggt ctg gac cac ttc caa aac agc aac ata gac cag agc ttc tgg gag 96 Gly Leu Asp His Phe Gln Asn Ser Asn Ile Asp Gln Ser Phe Trp Glu 20 25 30 acc ttt gga agt gct gag ccc acc aag acc cgc aag tcc ccg agc agc 144 Thr Phe Gly Ser Ala Glu Pro Thr Lys Thr Arg Lys Ser Pro Ser Ser 35 40 45 gac agc tgg acg tgc gcg gac acc tcc acc gag agg agg agc tcg gac 192 Asp Ser Trp Thr Cys Ala Asp Thr Ser Thr Glu Arg Arg Ser Ser Asp 50 55 60 agc tgg gag gtg tgg ggc tcg gcc tcc acc aac agg aac agc aac agc 240 Ser Trp Glu Val Trp Gly Ser Ala Ser Thr Asn Arg Asn Ser Asn Ser 65 70 75 80 gac ggc ggg gag ggc ggg gag ggc acc aag aag gca gtg ccg ccg gcc 288 Asp Gly Gly Glu Gly Gly Glu Gly Thr Lys Lys Ala Val Pro Pro Ala 85 90 95 gtg ccc act gat gat ggc tgg gac aac cag aac tgg tagggcccac 334 Val Pro Thr Asp Asp Gly Trp Asp Asn Gln Asn Trp 100 105 tgcgcccccg tccccagcgc ccccgggcga cttcgtgttt gcactctgcc ctcgtcgttc 394 ctcctccttc catttgaccc aagaatcagc aactgcagtg tgaggacagc gtctcgggag 454 gcacgaccct agggagaccc gggtgtgcgc ccgcctgcgc gttggggagt cttcggtgcg 514 tgggg 519 <210> 42 <211> 1630 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb2060 <220> <221> CDS <222> (1)..(1293) <400> 42 aat tct cca tat tct ctc tta gag gtg ctg tgt cct cga tgt gat att 48 Asn Ser Pro Tyr Ser Leu Leu Glu Val Leu Cys Pro Arg Cys Asp Ile 1 5 10 15 ggc ctt ttg gga ttg aaa agg aag cag aga aag tta aag gag ccc ttg 96 Gly Leu Leu Gly Leu Lys Arg Lys Gln Arg Lys Leu Lys Glu Pro Leu 20 25 30 cca aat gga aag aaa aaa agc acc aaa tta gag agt ctg agt gac ctg 144 Pro Asn Gly Lys Lys Lys Ser Thr Lys Leu Glu Ser Leu Ser Asp Leu 35 40 45 gag aga gct tta act gaa agc aag gag act gct tca gct atg gca act 192 Glu Arg Ala Leu Thr Glu Ser Lys Glu Thr Ala Ser Ala Met Ala Thr 50 55 60 ctt ggg gaa gct cgc cta agg gaa atg gaa gcc ttg cag tct ctc aga 240 Leu Gly Glu Ala Arg Leu Arg Glu Met Glu Ala Leu Gln Ser Leu Arg 65 70 75 80 ctc gcc aat gaa ggg aaa ttg ctg tcg cct ctc caa gat gtg gat ata 288 Leu Ala Asn Glu Gly Lys Leu Leu Ser Pro Leu Gln Asp Val Asp Ile 85 90 95 aaa atc tgc cta tgt cag aag gcc cca gct gcc cct atg att caa tgt 336 Lys Ile Cys Leu Cys Gln Lys Ala Pro Ala Ala Pro Met Ile Gln Cys 100 105 110 gaa ctc tgc agg gat gct ttc cac acc agt tgt gtg gcg gta ccc agt 384 Glu Leu Cys Arg Asp Ala Phe His Thr Ser Cys Val Ala Val Pro Ser 115 120 125 att tca cag ggc ctg cga atc tgg ctt tgt ccc cat tgt cgg agg tca 432 Ile Ser Gln Gly Leu Arg Ile Trp Leu Cys Pro His Cys Arg Arg Ser 130 135 140 gag aaa cct cca tta gag aaa att ctg ccc ctg ctc gcc tcc ctt cag 480 Glu Lys Pro Pro Leu Glu Lys Ile Leu Pro Leu Leu Ala Ser Leu Gln 145 150 155 160 cgt atc cga gtt cgc ctt cct gag gga gat gca ctt cga tat atg att 528 Arg Ile Arg Val Arg Leu Pro Glu Gly Asp Ala Leu Arg Tyr Met Ile 165 170 175 gaa aga acc gtg aac tgg cag cac aga gcc cag caa ctg ctt tcg tca 576 Glu Arg Thr Val Asn Trp Gln His Arg Ala Gln Gln Leu Leu Ser Ser 180 185 190 ggg aat ctt aaa ttt gtg caa gat cga gtg ggc tca gga ctg tta tat 624 Gly Asn Leu Lys Phe Val Gln Asp Arg Val Gly Ser Gly Leu Leu Tyr 195 200 205 agc aga tgg caa gcc tca gca gga cag gtg tca gac aca aac aag gta 672 Ser Arg Trp Gln Ala Ser Ala Gly Gln Val Ser Asp Thr Asn Lys Val 210 215 220 tct caa cct cct ggc aca aca tca ttt tct ttg cct gat gac tgg gac 720 Ser Gln Pro Pro Gly Thr Thr Ser Phe Ser Leu Pro Asp Asp Trp Asp 225 230 235 240 aac aga acc tca tat ttg cac tcc ccc ttc tca act gga cga agt tgt 768 Asn Arg Thr Ser Tyr Leu His Ser Pro Phe Ser Thr Gly Arg Ser Cys 245 250 255 atc ccc ctc cat ggt gtt agt cca gaa gtg aat gaa cta ttg atg gaa 816 Ile Pro Leu His Gly Val Ser Pro Glu Val Asn Glu Leu Leu Met Glu 260 265 270 gcc cag ctg ctc cag gta tcc ctt cct gaa att cag gaa ctt tac cag 864 Ala Gln Leu Leu Gln Val Ser Leu Pro Glu Ile Gln Glu Leu Tyr Gln 275 280 285 act tta ctt gca aag cca agc cct gct cag cag act gac cga agc tca 912 Thr Leu Leu Ala Lys Pro Ser Pro Ala Gln Gln Thr Asp Arg Ser Ser 290 295 300 cca gtg aga ccc agc agt gag aag aat gac tgt tgc cga ggg aag cga 960 Pro Val Arg Pro Ser Ser Glu Lys Asn Asp Cys Cys Arg Gly Lys Arg 305 310 315 320 gat gga att aac agt ctt gag aga aaa ctg aag aga cgc ctg gaa aga 1008 Asp Gly Ile Asn Ser Leu Glu Arg Lys Leu Lys Arg Arg Leu Glu Arg 325 330 335 gag ggc ctc tcc agt gag cgg tgg gaa cga gtt aag aaa atg cgg acc 1056 Glu Gly Leu Ser Ser Glu Arg Trp Glu Arg Val Lys Lys Met Arg Thr 340 345 350 ccc aaa aag aag aaa atc aaa ctg agc cac ccc aag gac atg aac aat 1104 Pro Lys Lys Lys Lys Ile Lys Leu Ser His Pro Lys Asp Met Asn Asn 355 360 365 ttc aag tta gag aga gag cgt agc tat gaa act cat tcc ctg ccc tca 1152 Phe Lys Leu Glu Arg Glu Arg Ser Tyr Glu Thr His Ser Leu Pro Ser 370 375 380 gac aca tcc tat tcc gaa cag gaa gac tct gag gat gaa gat gcc atc 1200 Asp Thr Ser Tyr Ser Glu Gln Glu Asp Ser Glu Asp Glu Asp Ala Ile 385 390 395 400 tgc cca gct gtg agc tgc ctg cag cca gaa gga gat gag gtc agt gag 1248 Cys Pro Ala Val Ser Cys Leu Gln Pro Glu Gly Asp Glu Val Ser Glu 405 410 415 gtt tgg gcc ata gag gac gtg ctt tcc ctt aac tca gaa acc ctg 1293 Val Trp Ala Ile Glu Asp Val Leu Ser Leu Asn Ser Glu Thr Leu 420 425 430 taagctagac ctcatctcgc tgggtctttc tcctctgctt atctgctttg agctgatctg 1353 aatggattgc tcagcccttc ccccactgat atctgtgctc ttaccatcgt tcctgggtct 1413 cctccctttt cttgaagaaa ctcttggtct tccctttttc cttttcttgc tcttccagct 1473 ttcaccttcc cggcgctaag aaggtgggag ggatgctgat gtgatgccct ttggcttact 1533 tatcacaggg cagcgctggc ttaaccccaa agcagacacg acttgaagaa tgactttgac 1593 tcggttgctt tggctagggg gccagctaca ctcaatt 1630 <210> 43 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 43 tttgaattcg ccaattttaa tcaaagtggg 30 <210> 44 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 44 tagcatctat gactttttgg ggcgttcaag tg 32 <210> 45 <211> 342 <212> DNA <213> Saccharomyces cerevisiae <220> <221> CDS <222> (1)..(342) <220> <221> domain <222> (1)..(342) <400> 45 gcc aat ttt aat caa agt ggg aat att gct gat agc tca ttg tcc ttc 48 Ala Asn Phe Asn Gln Ser Gly Asn Ile Ala Asp Ser Ser Leu Ser Phe 1 5 10 15 act ttc act aac agt agc aac ggt ccg aac ctc ata aca act caa aca 96 Thr Phe Thr Asn Ser Ser Asn Gly Pro Asn Leu Ile Thr Thr Gln Thr 20 25 30 aat tct caa gcg ctt tca caa cca att gcc tcc tct aac gtt cat gat 144 Asn Ser Gln Ala Leu Ser Gln Pro Ile Ala Ser Ser Asn Val His Asp 35 40 45 aac ttc atg aat aat gaa atc acg gct agt aaa att gat gat ggt aat 192 Asn Phe Met Asn Asn Glu Ile Thr Ala Ser Lys Ile Asp Asp Gly Asn 50 55 60 aat tca aaa cca ctg tca cct ggt tgg acg gac caa act gcg tat aac 240 Asn Ser Lys Pro Leu Ser Pro Gly Trp Thr Asp Gln Thr Ala Tyr Asn 65 70 75 80 gcg ttt gga atc act aca ggg atg ttt aat acc act aca atg gat gat 288 Ala Phe Gly Ile Thr Thr Gly Met Phe Asn Thr Thr Thr Met Asp Asp 85 90 95 gta tat aac tat cta ttc gat gat gaa gat acc cca cca aac cca aaa 336 Val Tyr Asn Tyr Leu Phe Asp Asp Glu Asp Thr Pro Pro Asn Pro Lys 100 105 110 aaa gag 342 Lys Glu <210> 46 <211> 609 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(606) <400> 46 atg aaa gcg tta acg gcc agg caa caa gag gtg ttt gat ctc atc cgt 48 Met Lys Ala Leu Thr Ala Arg Gln Gln Glu Val Phe Asp Leu Ile Arg 1 5 10 15 gat cac atc agc cag aca ggt atg ccg ccg acg cgt gcg gaa atc gcg 96 Asp His Ile Ser Gln Thr Gly Met Pro Pro Thr Arg Ala Glu Ile Ala 20 25 30 cag cgt ttg ggg ttc cgt tcc cca aac gcg gct gaa gaa cat ctg aag 144 Gln Arg Leu Gly Phe Arg Ser Pro Asn Ala Ala Glu Glu His Leu Lys 35 40 45 gcg ctg gca cgc aaa ggc gtt att gaa att gtt tcc ggc gca tca cgc 192 Ala Leu Ala Arg Lys Gly Val Ile Glu Ile Val Ser Gly Ala Ser Arg 50 55 60 ggg att cgt ctg ttg cag gaa gag gaa gaa ggg ttg ccg ctg gta ggt 240 Gly Ile Arg Leu Leu Gln Glu Glu Glu Glu Gly Leu Pro Leu Val Gly 65 70 75 80 cgt gtg gct gcc ggt gaa cca ctt ctg gcg caa cag cat att gaa ggt 288 Arg Val Ala Ala Gly Glu Pro Leu Leu Ala Gln Gln His Ile Glu Gly 85 90 95 cat tat cag gtc gat cct tcc tta ttc aag ccg aat gct gat ttc ctg 336 His Tyr Gln Val Asp Pro Ser Leu Phe Lys Pro Asn Ala Asp Phe Leu 100 105 110 ctg cgc gtc agc ggg atg tcg atg aaa gat atc ggc att atg gat ggt 384 Leu Arg Val Ser Gly Met Ser Met Lys Asp Ile Gly Ile Met Asp Gly 115 120 125 gac ttg ctg gca gtg cat aaa act cag gat gta cgt aac ggt cag gtc 432 Asp Leu Leu Ala Val His Lys Thr Gln Asp Val Arg Asn Gly Gln Val 130 135 140 gtt gtc gca cgt att gat gac gaa gtt acc gtt aag cgc ctg aaa aaa 480 Val Val Ala Arg Ile Asp Asp Glu Val Thr Val Lys Arg Leu Lys Lys 145 150 155 160 cag ggc aat aaa gtc gaa ctg ttg cca gaa aat agc gag ttt aaa cca 528 Gln Gly Asn Lys Val Glu Leu Leu Pro Glu Asn Ser Glu Phe Lys Pro 165 170 175 att gtc gtt gac ctt cgt cag cag agc ttc acc att gaa ggg ctg gcg 576 Ile Val Val Asp Leu Arg Gln Gln Ser Phe Thr Ile Glu Gly Leu Ala 180 185 190 gtt ggg gtt att cgc aac ggc gac tgg ctg taa 609 Val Gly Val Ile Arg Asn Gly Asp Trp Leu 195 200 <210> 47 <211> 10 <212> PRT <213> Human immunodeficiency virus <400> 47 Gln Leu Pro Pro Leu Glu Arg Leu Thr Leu 1 5 10 <210> 48 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 48 acagctgcca ccgattgaga gacttacgtt 30 <210> 49 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 49 tgtcgacggt ggctaactct ctgaatgcaa 30 <210> 50 <211> 1080 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: DNA cording for LexA-GAL4 fusion protein <220> <221> CDS <222> (1)..(1077) <400> 50 atg aaa gcg tta cag ctg cca ccg att gag aga ctt acg tta acg gcc 48 Met Lys Ala Leu Gln Leu Pro Pro Ile Glu Arg Leu Thr Leu Thr Ala 1 5 10 15 agg caa caa gag gtg ttt gat ctc atc cgt gat cac atc agc cag aca 96 Arg Gln Gln Glu Val Phe Asp Leu Ile Arg Asp His Ile Ser Gln Thr 20 25 30 ggt atg ccg ccg acg cgt gcg gaa atc gcg cag cgt ttg ggg ttc cgt 144 Gly Met Pro Pro Thr Arg Ala Glu Ile Ala Gln Arg Leu Gly Phe Arg 35 40 45 tcc cca aac gcg gct gaa gaa cat ctg aag gcg ctg gca cgc aaa ggc 192 Ser Pro Asn Ala Ala Glu Glu His Leu Lys Ala Leu Ala Arg Lys Gly 50 55 60 gtt att gaa att gtt tcc ggc gca tca cgc ggg att cgt ctg ttg cag 240 Val Ile Glu Ile Val Ser Gly Ala Ser Arg Gly Ile Arg Leu Leu Gln 65 70 75 80 gaa gag gaa gaa ggg ttg ccg ctg gta ggt cgt gtg gct gcc ggt gaa 288 Glu Glu Glu Glu Gly Leu Pro Leu Val Gly Arg Val Ala Ala Gly Glu 85 90 95 cca ctt ctg gcg caa cag cat att gaa ggt cat tat cag gtc gat cct 336 Pro Leu Leu Ala Gln Gln His Ile Glu Gly His Tyr Gln Val Asp Pro 100 105 110 tcc tta ttc aag ccg aat gct gat ttc ctg ctg cgc gtc agc ggg atg 384 Ser Leu Phe Lys Pro Asn Ala Asp Phe Leu Leu Arg Val Ser Gly Met 115 120 125 tcg atg aaa gat atc ggc att atg gat ggt gac ttg ctg gca gtg cat 432 Ser Met Lys Asp Ile Gly Ile Met Asp Gly Asp Leu Leu Ala Val His 130 135 140 aaa act cag gat gta cgt aac ggt cag gtc gtt gtc gca cgt att gat 480 Lys Thr Gln Asp Val Arg Asn Gly Gln Val Val Val Ala Arg Ile Asp 145 150 155 160 gac gaa gtt acc gtt aag cgc ctg aaa aaa cag ggc aat aaa gtc gaa 528 Asp Glu Val Thr Val Lys Arg Leu Lys Lys Gln Gly Asn Lys Val Glu 165 170 175 ctg ttg cca gaa aat agc gag ttt aaa cca att gtc gtt gac ctt cgt 576 Leu Leu Pro Glu Asn Ser Glu Phe Lys Pro Ile Val Val Asp Leu Arg 180 185 190 cag cag agc ttc acc att gaa ggg ctg gcg gtt ggg gtt att cgc aac 624 Gln Gln Ser Phe Thr Ile Glu Gly Leu Ala Val Gly Val Ile Arg Asn 195 200 205 ggc gac tgg ctg gaa ttc gcc aat ttt aat caa agt ggg aat att gct 672 Gly Asp Trp Leu Glu Phe Ala Asn Phe Asn Gln Ser Gly Asn Ile Ala 210 215 220 gat agc tca ttg tcc ttc act ttc act aac agt agc aac ggt ccg aac 720 Asp Ser Ser Leu Ser Phe Thr Phe Thr Asn Ser Ser Asn Gly Pro Asn 225 230 235 240 ctc ata aca act caa aca aat tct caa gcg ctt tca caa cca att gcc 768 Leu Ile Thr Thr Gln Thr Asn Ser Gln Ala Leu Ser Gln Pro Ile Ala 245 250 255 tcc tct aac gtt cat gat aac ttc atg aat aat gaa atc acg gct agt 816 Ser Ser Asn Val His Asp Asn Phe Met Asn Asn Glu Ile Thr Ala Ser 260 265 270 aaa att gat gat ggt aat aat tca aaa cca ctg tca cct ggt tgg acg 864 Lys Ile Asp Asp Gly Asn Asn Ser Lys Pro Leu Ser Pro Gly Trp Thr 275 280 285 gac caa act gcg tat aac gcg ttt gga atc act aca ggg atg ttt aat 912 Asp Gln Thr Ala Tyr Asn Ala Phe Gly Ile Thr Thr Gly Met Phe Asn 290 295 300 acc act aca atg gat gat gta tat aac tat cta ttc gat gat gaa gat 960 Thr Thr Thr Met Asp Asp Val Tyr Asn Tyr Leu Phe Asp Asp Glu Asp 305 310 315 320 acc cca cca aac cca aaa aaa gag atc tct atg gct tac cca tac gat 1008 Thr Pro Pro Asn Pro Lys Lys Glu Ile Ser Met Ala Tyr Pro Tyr Asp 325 330 335 gtt cca gat tac gct agc ttg ggt ggt cat atg gcc atg gcg gcc gct 1056 Val Pro Asp Tyr Ala Ser Leu Gly Gly His Met Ala Met Ala Ala Ala 340 345 350 cga gtc gac ctg cag cca agc taa 1080 Arg Val Asp Leu Gln Pro Ser 355 <210> 51 <211> 1152 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: DNA cording for 'BraC protein derived from Pseudomonas aeruginosa BraC （修正しました） <220> <221> CDS <222> (1)..(1149) <400> 51 atg gct aag atc tct ccc ggg ctc gag ctc atg aag aag ggt act cag 48 Met Ala Lys Ile Ser Pro Gly Leu Glu Leu Met Lys Lys Gly Thr Gln 1 5 10 15 cgt cta tcc cgc ctg ttc gcc gcg atg gcc att gcc ggg ttc gcc agc 96 Arg Leu Ser Arg Leu Phe Ala Ala Met Ala Ile Ala Gly Phe Ala Ser 20 25 30 tac tcc atg gcc gcc gac acc atc aag atc gcc ctg gct ggc ccg gtc 144 Tyr Ser Met Ala Ala Asp Thr Ile Lys Ile Ala Leu Ala Gly Pro Val 35 40 45 acc ggt ccg gta gcc cag tac ggc gac atg cag cgc gcc ggt gcg ctg 192 Thr Gly Pro Val Ala Gln Tyr Gly Asp Met Gln Arg Ala Gly Ala Leu 50 55 60 atg gca atc gaa cag atc aac aag gca ggc ggc gtg aac ggc gcg caa 240 Met Ala Ile Glu Gln Ile Asn Lys Ala Gly Gly Val Asn Gly Ala Gln 65 70 75 80 ctc gaa ggc gtg atc tac gac gac gcc tgc gat ccc aag cag gcc gtg 288 Leu Glu Gly Val Ile Tyr Asp Asp Ala Cys Asp Pro Lys Gln Ala Val 85 90 95 gcg gtc gcc aac aag gtg gtc aac gac ggc gtc aag ttc gtg gtc ggt 336 Ala Val Ala Asn Lys Val Val Asn Asp Gly Val Lys Phe Val Val Gly 100 105 110 cat gtc tgc tcc agc tcc acc caa ccc gcc acc gac atc tac gaa gac 384 His Val Cys Ser Ser Ser Thr Gln Pro Ala Thr Asp Ile Tyr Glu Asp 115 120 125 gaa ggc gtg ctg atg atc acc ccg tcg gcc acc gcc ccg gaa atc acc 432 Glu Gly Val Leu Met Ile Thr Pro Ser Ala Thr Ala Pro Glu Ile Thr 130 135 140 tcg cgc ggc tac aag ctg atc ttc cgc acc atc ggc ctg gac aac atg 480 Ser Arg Gly Tyr Lys Leu Ile Phe Arg Thr Ile Gly Leu Asp Asn Met 145 150 155 160 cag ggc ccg gtg gcc ggc aag ttc atc gcc gaa cgc tac aag gac aag 528 Gln Gly Pro Val Ala Gly Lys Phe Ile Ala Glu Arg Tyr Lys Asp Lys 165 170 175 acc atc gcg gta ctg cac gac aag cag cag tac ggc gaa ggc atc gcc 576 Thr Ile Ala Val Leu His Asp Lys Gln Gln Tyr Gly Glu Gly Ile Ala 180 185 190 acc gag gtg aag aag acc gtg gaa gac gcc ggc atc aag gtt gcc gtc 624 Thr Glu Val Lys Lys Thr Val Glu Asp Ala Gly Ile Lys Val Ala Val 195 200 205 ttc gaa ggc ctg aac gcc ggc gac aag gac ttc aac gcg ctg atc agc 672 Phe Glu Gly Leu Asn Ala Gly Asp Lys Asp Phe Asn Ala Leu Ile Ser 210 215 220 aag ctg aag aaa gcc ggc gtg cag ttc gtc tac ttc ggc ggc tac cac 720 Lys Leu Lys Lys Ala Gly Val Gln Phe Val Tyr Phe Gly Gly Tyr His 225 230 235 240 cca gaa atg ggc ctg ctg ctg cgc cag gcc aag cag gcc ggg ctg gac 768 Pro Glu Met Gly Leu Leu Leu Arg Gln Ala Lys Gln Ala Gly Leu Asp 245 250 255 gcg cgc ttc atg ggc ccg gaa ggg gtc ggc aac agc gaa atc acc gcg 816 Ala Arg Phe Met Gly Pro Glu Gly Val Gly Asn Ser Glu Ile Thr Ala 260 265 270 atc gcc ggc gac gct tcg gaa ggc atg ctg gcg acc ctg ccg cgc gcc 864 Ile Ala Gly Asp Ala Ser Glu Gly Met Leu Ala Thr Leu Pro Arg Ala 275 280 285 ttc gag cag gat ccg aag aac aag gcc ctg atc gac gcc ttc aag gcg 912 Phe Glu Gln Asp Pro Lys Asn Lys Ala Leu Ile Asp Ala Phe Lys Ala 290 295 300 aag aac cag gat ccg agc ggc atc ttc gtc ctg ccc gcc tac tcc gcg 960 Lys Asn Gln Asp Pro Ser Gly Ile Phe Val Leu Pro Ala Tyr Ser Ala 305 310 315 320 gtc aca gtg atc gcc aag ggc atc gag aaa gcc ggc gag gcc gat ccg 1008 Val Thr Val Ile Ala Lys Gly Ile Glu Lys Ala Gly Glu Ala Asp Pro 325 330 335 gag aag gtc gcc gag gcc ctg cgc gcc aac acc ttc gag act ccc acc 1056 Glu Lys Val Ala Glu Ala Leu Arg Ala Asn Thr Phe Glu Thr Pro Thr 340 345 350 ggg aac ctc ggg ttc gac gag aag ggc gac ctg aag aac ttc gac ttc 1104 Gly Asn Leu Gly Phe Asp Glu Lys Gly Asp Leu Lys Asn Phe Asp Phe 355 360 365 acc gtc tac gag tgg cac aag gac gcc acc cgg acc gag gtc aag taa 1152 Thr Val Tyr Glu Trp His Lys Asp Ala Thr Arg Thr Glu Val Lys 370 375 380 <210> 52 <211> 12 <212> PRT <213> Simian virus 40 <400> 52 Ser Glu Pro Pro Lys Lys Lys Arg Lys Val Glu Thr 1 5 10 <210> 53 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 53 ctagcgagcc tccaaaaaag aagagaaagg tcgaaac 37 <210> 54 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <220> <223> Number of chain: single-strand, topology: linear <400> 54 gctcggaggt tttttcttct ctttccagct ttggtac 37 <210> 55 <211> 419 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized promoter sequence with LexA operator <220> <223> genomic DNA <400> 55 tcgactgctg tatataaaac cagtggttat atgtacagta ctgctgtata taaaaccagt 60 ggttatatgt acagtacgtc gagggaatca aattaacaac cataggatga taatgcgatt 120 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta 180 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg 240 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct 300 ctatacttta acgtcaagga gaaaaaacta taatgactaa atctcattca gaagaagtga 360 ttgtacctga gttcaattct agcgcaaagg aattaccaag accattggcc gaaaagtgc 419 <210> 56 <211> 12 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 56 aattgaccac cc 12 <210> 57 <211> 12 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 57 ctggtgggtt aa 12 <210> 58 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 58 ctagcttggg tggaattcat atggc 25 <210> 59 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 59 gaacccacct taagtatacg gtac 24 <210> 60 <211> 11 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 60 ctgcatgcac c 11 <210> 61 <211> 14 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized oligonucleotide sequence <400> 61 atggacgtac gtgg 14 <210> 62 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 62 ctattcgatg atgaagatac cccaccaaac cc 32 <210> 63 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 63 gaaattcgcc cggaattagc ttggctgcag 30 <210> 64 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 64 ctattcgatg atgaagatac cccaccaaac cc 32 <210> 65 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 65 gaaattcgcc cggaattagc ttggctgcag 30 <210> 66 <211> 539 <212> DNA <213> Homo sapiens <400> 66 gcaattttgc cttctgtact ttgtgcaggt tgacctacca tggggtctcc ccatgtaagg 60 tgactgcaga gaaattaatg gacttacgaa atgaatacct gccagcggat gaggctaata 120 aaagactttt ggatcaaagg tatggtaaga gagtgattca gaaggcactg gaagagatgg 180 aaagtaagga gtggctagag aagaactcca agagctgccc atgttgtgga actcccatag 240 agaaattaga cggatgttac aagatgacat gttctggctg tatgcaatat ttctgttgga 300 tttgcatggg ttctctctct agaacaaacc cttacaaaca tttcaatgac cctggttcac 360 catgttttaa ccggctgttt tatgctgtgg atgttgacaa caatatttgg gaagatgaag 420 tagaagacta gttaactact gctcnagata tggaagtgga ttgtttttcc cnaatctccg 480 tccagtncca aagtaacttt gcgggatatt tagggtctat cnttcactct cctgcgtaa 539 <210> 67 <211> 534 <212> DNA <213> Homo sapiens <400> 67 gaattcctga tgaggtttta caaagtattt tggatcaata ctccaacaaa tcagaaagcc 60 agaaagagga tcctttcaat attgcagaac cacgagtgga tttacacacc tcaggagaac 120 actcagaatt ggttcaagaa gaaaatttga gcccaggcac ccaaacacct tcaaatgata 180 aagcaagtat gttgcaagaa tactccaaat acctccaaca ggcttttgaa aaatccacta 240 atgcaagttt tactcttgga cacggtttcc aatttgtcag tttgtcttca cctctccaca 300 accacacttt gtttccagaa aaacaaatat acactacgtc tcctttggag tgtggtttcg 360 gccaatctgt tacctcagtg ttgccatctt cattgccaaa gcctcctttt gggatgttgt 420 ttggatctca gccaggtctt tatttgtctg ctttggatgc tacacatcag cagttgacac 480 cttcccagga gctggatgat ctgatagatt ctcagaagaa cttagagact tcat 534 <210> 68 <211> 453 <212> DNA <213> Homo sapiens <400> 68 ccttgctggg ggttcgggga gcgcttggac cccggcttct gggacgcgtc aggagaaagg 60 agcactggct ttgctttcat caggccaaag atgcctttct ttgggaatac gttcagtccg 120 aagaagacac ctcctcggaa gtcggcatct ctctccaacc tgcattcttt ggatcgatca 180 acccgggagg tggagctggg cttggaatac ggatccccga ctatgaacct ggcagggcaa 240 agcctgaagt ttgaaaatgg ccagtggata gcagagacag gggttagtgg cggtgtggac 300 cggagggaag ttcagcgcct tcgcaggcgg aaccagcagt tggaggaaga gaacaatctc 360 ttgcggctga aagtggacat cttattagac atgctttcag agtccactgc cgaatcccac 420 ttaatggaga aggaactgga tgaactgagg atc 453 <210> 69 <211> 535 <212> DNA <213> Homo sapiens <400> 69 ggatccgctc agaaatgggc cagcttcggc aggagaacga gctgctgcag aacaagttac 60 ataatgctgt gcaaatgaag caaaaagaca agcagaatat cagccagttg gagaaaaagc 120 taaaagctga gcaggaagcc cgaagttttg tagagaaaca gttaatggaa gagaaaaaga 180 ggaagaagtt agaagaagcc actgctgccc gggctgttgc gtttgctgct gcatctaggg 240 gagaatgcac cgaaacctta cggaatcgga tcagagaact agaagcagag ggcaagaagc 300 tcacgatgga catgaaggtg aaagaagacc aaatcagaga actagaacta aaagtccagg 360 agcttcggaa atataaggaa aatgagaagg acactgaggt gttaatgtca gccctctcag 420 ccatgcaaga caaaacacag cacctggaga acagcttaag tgcagagacg agaatcaagc 480 tggacctgtt ctccgcactg ggcgatgcaa agcggcagct cgagattgcc caagg 535 <210> 70 <211> 578 <212> DNA <213> Homo sapiens <400> 70 gaattcctca agacaccatt ccagagtatc ctgagctcca gctggaccct aaattggatc 60 ctctttttgc tgagagtccc ctaatgaaca ttgaggttgt tgaggtcctc acactgaacc 120 aggaggtggc tggtccccgg aatgcccaga tccaggccct atatgctgaa gatggaagcc 180 tgagtgcaga tgcccccagt gagcaggtcc aacagcaggg caagcatcca ggtgaccctg 240 aggccgcgcg ccagaagttc cggcagttcc gttataagga catgacaggt ccccgggagg 300 ccctggacca gctccgagag ctgtgtcacc agtggctaca gcctaaggca cgctccaagg 360 agcagatcct ggagctgctg gtgctggagc agttcctaag tgcactgcct gtgaaactcc 420 cgacatgggt ggaatcgcng cacccaaaaa atgccaanaa gtggtggccc tggtaaaagg 480 tgtgacctgg attctgaaga agaagtactt cctgcaggan aactgccnag ggcaccnctg 540 ctgctcaagg tcnctgcccn caggaagaaa aacanaag 578 <210> 71 <211> 564 <212> DNA <213> Homo sapiens <400> 71 gtttcaacta ttcccttccc aaaaaagcct gtagaacatg caagaaaaaa ttccattcag 60 cctgcttgta caaatggttt acatctagca acaaatccac ttgtccactg tgtcgtgaga 120 cgtttttctg agattttttc actggaaggg tccctgaagt acatcaaaca aaggcattgg 180 gtttggtccg tctgaaagtg tggtgtgggg aagccagtga gcattacttt aaataggacc 240 tctctggaaa atattttggt aatgtaataa tcctaaaatc aggttactaa cttagattgc 300 ttgtaaatgt ttggaacttc ttttacaaaa gaatattaca tatttgagag aaaatatttc 360 attaatggtt aatttcttgt atatttaaaa ataaatatga aaaaccccta aattacagaa 420 ttnggnattt gtgaaaagnt gcacaactat attattgana aacccattcc gtttgttgat 480 ccgtntttan aatatccaag tgaagcaaaa cgaactgaga gagctnttta catctataga 540 tatttaagcc tattattagt atng 564 <210> 72 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 72 gccttctgta ctttgtgcag g 21 <210> 73 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 73 tttaaccggc tgttttatgc 20 <210> 74 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 74 cactccttac tttccatctc ttccagtgcc 30 <210> 75 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 75 cagaaagcca gaaagaggat cc 22 <210> 76 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 76 gtcttcacct ctccacaacc 20 <210> 77 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 77 gcctgttgga ggtatttgga gtattcttgc 30 <210> 78 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 78 caggagaaag gagcactggc 20 <210> 79 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 79 ccaaagatgc ctttctttgg 20 <210> 80 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 80 ggttcatagt cggggatccg tattcc 26 <210> 81 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 81 ataatgctgt gcaaatgaag c 21 <210> 82 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 82 agcagaatat cagccagttg g 21 <210> 83 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 83 gcttctagtt ctctgatccg attccgtaag 30 <210> 84 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 84 ttgaggtcct cacactgaac c 21 <210> 85 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 85 tccgttataa ggacatgaca gg 22 <210> 86 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 86 cactcaggct tccatcttca gcatataggg 30 <210> 87 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 87 ctattccctt cccaaaaaag c 21 <210> 88 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 88 cccattccgt ttgttgatcc 20 <210> 89 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 89 cgtctcacga cacagtggac aagtgg 26 <210> 90 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Artificially synthesized primer sequence <400> 90 cgctgttttg acctccatag aagacacc 28 <210> 91 <211> 215 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Upstream region of cloning site of pCMV SPORT2 <400> 91 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag 60 cctccggact ctagcctagg ccgcggagcg gataacaatt tcacacagga aacagctatg 120 accactaggc ttttgcaaaa agctatttag gtgacactat agaaggtacg cctgcaggta 180 ccggtccgga attcccgggt cgacccacgc gtccg 215 <210> 92 <211> 3092 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb030 <220> <221> CDS <222> (237)..(1658) <400> 92 gaagaggcgg cgcgccggtt gagcagggcg tttctaggcc tggtcggctg gcggcgatgg 60 caggattttc ataatatatg tagtatgagt tccacatctt ggcctcttac ccagcttcag 120 cagtctcagc tccaccagtt agagaataaa tgggatttgc atgaactcca ctctgagctg 180 aaatacagac tgcggtgtta atatcctttt cttctgattg ttattaacag gtcctt atg 239 Met 1 tcg tca gaa gat cga gaa gct cag gag gat gaa ttg ctg gcc ctg gca 287 Ser Ser Glu Asp Arg Glu Ala Gln Glu Asp Glu Leu Leu Ala Leu Ala 5 10 15 agt att tac gat gga gat gaa ttt aga aaa gca gag tct gtc caa ggt 335 Ser Ile Tyr Asp Gly Asp Glu Phe Arg Lys Ala Glu Ser Val Gln Gly 20 25 30 gga gaa acc agg atc tat ttg gat ttg cca cag aat ttc aag ata ttt 383 Gly Glu Thr Arg Ile Tyr Leu Asp Leu Pro Gln Asn Phe Lys Ile Phe 35 40 45 gtg agc ggc aat tca aat gag tgt ctc cag aat agt ggc ttt gaa tac 431 Val Ser Gly Asn Ser Asn Glu Cys Leu Gln Asn Ser Gly Phe Glu Tyr 50 55 60 65 acc att tgc ttt ctg cct cca ctt gtg ctg aac ttt gaa ctg cca cca 479 Thr Ile Cys Phe Leu Pro Pro Leu Val Leu Asn Phe Glu Leu Pro Pro 70 75 80 gat tat cca tcc tct tcc cca cct tca ttc aca ctt agt ggc aaa tgg 527 Asp Tyr Pro Ser Ser Ser Pro Pro Ser Phe Thr Leu Ser Gly Lys Trp 85 90 95 ctg tca cca act cag cta tct gct cta tgc aag cac tta gac aac cta 575 Leu Ser Pro Thr Gln Leu Ser Ala Leu Cys Lys His Leu Asp Asn Leu 100 105 110 tgg gaa gaa cac cgt ggc agc gtg gtc ctg ttt gcc tgg atg caa ttt 623 Trp Glu Glu His Arg Gly Ser Val Val Leu Phe Ala Trp Met Gln Phe 115 120 125 ctt aag gaa gag acc cta gca tac ttg aat att gtc tct cct ttt gag 671 Leu Lys Glu Glu Thr Leu Ala Tyr Leu Asn Ile Val Ser Pro Phe Glu 130 135 140 145 ctc aag att ggt tct cag aaa aaa gtg cag aga agg aca gct caa gct 719 Leu Lys Ile Gly Ser Gln Lys Lys Val Gln Arg Arg Thr Ala Gln Ala 150 155 160 tct ccc aac aca gag cta gat ttt gga gga gct gct gga tct gat gta 767 Ser Pro Asn Thr Glu Leu Asp Phe Gly Gly Ala Ala Gly Ser Asp Val 165 170 175 gac caa gag gaa att gtg gat gag aga gca gtg cag gat gtg gaa tca 815 Asp Gln Glu Glu Ile Val Asp Glu Arg Ala Val Gln Asp Val Glu Ser 180 185 190 ctg tca aat ctg atc cag gaa atc ttg gac ttt gat caa gct cag cag 863 Leu Ser Asn Leu Ile Gln Glu Ile Leu Asp Phe Asp Gln Ala Gln Gln 195 200 205 ata aaa tgc ttt aat agt aaa ttg ttc ctg tgc agt atc tgt ttc tgt 911 Ile Lys Cys Phe Asn Ser Lys Leu Phe Leu Cys Ser Ile Cys Phe Cys 210 215 220 225 gag aag ctg ggt agt gaa tgc atg tac ttc ttg gag tgc agg cat gtg 959 Glu Lys Leu Gly Ser Glu Cys Met Tyr Phe Leu Glu Cys Arg His Val 230 235 240 tac tgc aaa gcc tgt ctg aag gac tac ttt gaa atc cag atc aga gat 1007 Tyr Cys Lys Ala Cys Leu Lys Asp Tyr Phe Glu Ile Gln Ile Arg Asp 245 250 255 ggc cag gtt caa tgc ctc aac tgc cca gaa cca aag tgc cct tcg gtg 1055 Gly Gln Val Gln Cys Leu Asn Cys Pro Glu Pro Lys Cys Pro Ser Val 260 265 270 gcc act cct ggt cag gtc aaa gag tta gtg gaa gca gag tta ttt gcc 1103 Ala Thr Pro Gly Gln Val Lys Glu Leu Val Glu Ala Glu Leu Phe Ala 275 280 285 cgt tat gac cgc ctt ctc ctc cag tcc tcc ttg gac ctg atg gca gat 1151 Arg Tyr Asp Arg Leu Leu Leu Gln Ser Ser Leu Asp Leu Met Ala Asp 290 295 300 305 gtg gtg tac tgc ccc cgg ccg tgc tgc cag ctg cct gtg atg cag gaa 1199 Val Val Tyr Cys Pro Arg Pro Cys Cys Gln Leu Pro Val Met Gln Glu 310 315 320 cct ggc tgc acc atg ggt atc tgc tcc agc tgc aat ttt gcc ttc tgt 1247 Pro Gly Cys Thr Met Gly Ile Cys Ser Ser Cys Asn Phe Ala Phe Cys 325 330 335 act ttg tgc agg ttg acc tac cat ggg gtc tcc cca tgt aag gtg act 1295 Thr Leu Cys Arg Leu Thr Tyr His Gly Val Ser Pro Cys Lys Val Thr 340 345 350 gca gag aaa tta atg gac tta cga aat gaa tac ctg caa gcg gat gag 1343 Ala Glu Lys Leu Met Asp Leu Arg Asn Glu Tyr Leu Gln Ala Asp Glu 355 360 365 gct aat aaa aga ctt ttg gat caa agg tat ggt aag aga gtg att cag 1391 Ala Asn Lys Arg Leu Leu Asp Gln Arg Tyr Gly Lys Arg Val Ile Gln 370 375 380 385 aag gca ctg gaa gag atg gaa agt aag gag tgg cta gag aag aac tca 1439 Lys Ala Leu Glu Glu Met Glu Ser Lys Glu Trp Leu Glu Lys Asn Ser 390 395 400 aag agc tgc cca tgt tgt gga act ccc ata gag aaa tta gac gga tgt 1487 Lys Ser Cys Pro Cys Cys Gly Thr Pro Ile Glu Lys Leu Asp Gly Cys 405 410 415 aac aag atg aca tgt act ggc tgt atg caa tat ttc tgt tgg att tgc 1535 Asn Lys Met Thr Cys Thr Gly Cys Met Gln Tyr Phe Cys Trp Ile Cys 420 425 430 atg ggt tct ctc tct aga gca aac cct tac aaa cat ttc aat gac cct 1583 Met Gly Ser Leu Ser Arg Ala Asn Pro Tyr Lys His Phe Asn Asp Pro 435 440 445 ggt tca cca tgt ttt aac cgg ctg ttt tat gct gtg gat gtt gac gac 1631 Gly Ser Pro Cys Phe Asn Arg Leu Phe Tyr Ala Val Asp Val Asp Asp 450 455 460 465 gat att tgg gaa gat gag gta gaa gac tagttaacta ctgctcaaga 1678 Asp Ile Trp Glu Asp Glu Val Glu Asp 470 tatggaagtg gattgttttt ccctaatctt ccgtcaagta cacaaagtaa ctttgcggga 1738 tatttagggt actattcatt cactcttcct gcgtagaaga tatggaagaa cgaggtttat 1798 attttcatgt ggtactactg aagaaggtgc attgatacat ttttaaatgt aagttgagaa 1858 aaatttataa gccaaaggtt cagaaaatta aactacagaa tattaaatat tataatgtgc 1918 ccaaagctct gaatagttaa aaattaaata tttattttct tccccaagct ttaggtaagg 1978 agaagagggg tcaagagtta aacttagaga ccctttgtct ctgagaagca tccttctaag 2038 acattctgtt ggagttccct cagtactatt ccttacaact ggagtgggta gaagccttat 2098 gaaaattata ctgacaacct gatctcgttt actccatgtt aatcacattc ctaccaacct 2158 aaatttctgc caagtcagtt ctctttggag gaagcccatt actccataac tgactggatg 2218 gtccagtgtc attttgatct gcttttcaga atggaaattt ataatataaa tatatgtttt 2278 aataccacaa acactgtggg gcacttgata tttattagtg gtgtatatgg tccactggtt 2338 tcaggccaaa tctagaattt agtgatactg gctcagaaga atttaagttc tattcagctt 2398 tcctgggtgt tagaacctag attcaaaatg gcttgtcttt gctacttttg ttccacattc 2458 tctctcttta cctttgccct accttctgtt tgtaaggatg attttaatat taattccagg 2518 aacacatgtt tttatttcct ctgctggaat aggagaaagc ttaatatatt cccaagctga 2578 atgttcttga attatatctg catttaccac tgtatatgca tatagtgaca gtataacctg 2638 tctatactac agtagttcca gtcctaactt tctgaattat ttttaaagat cttctctaac 2698 aagctatggg aatttggctt catactcttt ctttgcaaca gcagtgttct gggtgataat 2758 tttgaattga tacctgttcc tttttctggg ttttgttggc tttttgaaaa attgtctttc 2818 cttatcattg gtgggaggct tggtagcaaa gtaacatttt ttggaaaaga ggacagaaaa 2878 attgaactac agcttgagaa cgtattcttt ttttcctact ttgttattgc aaattgagga 2938 atcactttta actgttttag gtgtgtgtgt ccagagtgag caaggattat gtttttggat 2998 tgtcaaagag gatgcttagt cttaaaataa aaataaattt aaaaatcatc ttataattaa 3058 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 3092 <210> 93 <211> 3041 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb044-1 <220> <221> CDS <222> (48)..(2732) <400> 93 cgacggcggc tgcggcggcg gcgggacccc caggcctcct ccggggt atg aaa atc 56 Met Lys Ile 1 ggc agt ggg ttc ctg agt ggc ggc gga ggt acc ggc agt agc ggt ggt 104 Gly Ser Gly Phe Leu Ser Gly Gly Gly Gly Thr Gly Ser Ser Gly Gly 5 10 15 agc ggc tcc ggc ggc ggt ggt agt ggc ggc ggc ggc ggc ggc ggc agc 152 Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Gly Ser 20 25 30 35 agc ggc agg agg gca gag atg gaa ccc acc ttt ccc cag ggt atg gtt 200 Ser Gly Arg Arg Ala Glu Met Glu Pro Thr Phe Pro Gln Gly Met Val 40 45 50 atg ttc aac cac cgt ctt ccc ccg gtc acc agc ttc acc cgg ccg gcg 248 Met Phe Asn His Arg Leu Pro Pro Val Thr Ser Phe Thr Arg Pro Ala 55 60 65 ggg tcg gcc gcc cct ccc ccg caa tgc gtg tta tcc tcc tct acc tcc 296 Gly Ser Ala Ala Pro Pro Pro Gln Cys Val Leu Ser Ser Ser Thr Ser 70 75 80 gca gcc ccg gcc gct gag ccc ccc cct ccg cca gcc ccg gac atg act 344 Ala Ala Pro Ala Ala Glu Pro Pro Pro Pro Pro Ala Pro Asp Met Thr 85 90 95 ttc aag aag gag ccg gcg gcg tca gcc gcg gcc ttc ccc tcg cag agg 392 Phe Lys Lys Glu Pro Ala Ala Ser Ala Ala Ala Phe Pro Ser Gln Arg 100 105 110 115 acc tcc tgg ggg ttc ttg cag tct ttg gtt acc atc aaa cag gag aaa 440 Thr Ser Trp Gly Phe Leu Gln Ser Leu Val Thr Ile Lys Gln Glu Lys 120 125 130 ccc gcg gat cct gag gag cag cag tcc cac cac cac cat cac cac cac 488 Pro Ala Asp Pro Glu Glu Gln Gln Ser His His His His His His His 135 140 145 cac tat ggg ggg ctg ttc gct gga gct gaa gag agg tct cca ggc cta 536 His Tyr Gly Gly Leu Phe Ala Gly Ala Glu Glu Arg Ser Pro Gly Leu 150 155 160 gga ggc ggt gaa ggg ggg agt cac ggc gtc atc cag gac ctc agt att 584 Gly Gly Gly Glu Gly Gly Ser His Gly Val Ile Gln Asp Leu Ser Ile 165 170 175 ctc cac cag cat gtc cag cag caa cca gcc cag cac cac cgt gac gta 632 Leu His Gln His Val Gln Gln Gln Pro Ala Gln His His Arg Asp Val 180 185 190 195 tta ctc agc agc agt agc agg act gat gac cac cat ggc act gag gag 680 Leu Leu Ser Ser Ser Ser Arg Thr Asp Asp His His Gly Thr Glu Glu 200 205 210 cca aag cag gac act aat gtc aaa aag gca aaa agg cca aag cca gaa 728 Pro Lys Gln Asp Thr Asn Val Lys Lys Ala Lys Arg Pro Lys Pro Glu 215 220 225 tct cag gga atc aaa gcc aag agg aag cca agt gca tct tcc aaa cct 776 Ser Gln Gly Ile Lys Ala Lys Arg Lys Pro Ser Ala Ser Ser Lys Pro 230 235 240 tct ttg gtt gga gat gga gaa ggt gcc atc ctc tcc cca agt cag aaa 824 Ser Leu Val Gly Asp Gly Glu Gly Ala Ile Leu Ser Pro Ser Gln Lys 245 250 255 cct cat atc tgt gat cac tgt agt gct gct ttc cga agc tcc tat cac 872 Pro His Ile Cys Asp His Cys Ser Ala Ala Phe Arg Ser Ser Tyr His 260 265 270 275 ctg cgg aga cat gtc ctc att cat aca gga gaa aga cct ttc cag tgc 920 Leu Arg Arg His Val Leu Ile His Thr Gly Glu Arg Pro Phe Gln Cys 280 285 290 agc cag tgt agt atg ggt ttc att cag aaa tac cta cta cag aga cat 968 Ser Gln Cys Ser Met Gly Phe Ile Gln Lys Tyr Leu Leu Gln Arg His 295 300 305 gag aaa att cat agt aga gag aag cca ttt gga tgt gat cag tgc agc 1016 Glu Lys Ile His Ser Arg Glu Lys Pro Phe Gly Cys Asp Gln Cys Ser 310 315 320 atg aag ttt att cag aag tac cat atg gag aga cac aag agg aca cat 1064 Met Lys Phe Ile Gln Lys Tyr His Met Glu Arg His Lys Arg Thr His 325 330 335 agt gga gaa aag cca tat aag tgt gac act tgc caa cag tat ttt tca 1112 Ser Gly Glu Lys Pro Tyr Lys Cys Asp Thr Cys Gln Gln Tyr Phe Ser 340 345 350 355 agg act gat aga ttg ttg aag cac agg cgc aca tgt ggt gaa gtc ata 1160 Arg Thr Asp Arg Leu Leu Lys His Arg Arg Thr Cys Gly Glu Val Ile 360 365 370 gtt aaa gga gcc act agt gca gaa cct ggg tca tca aac cat acc aat 1208 Val Lys Gly Ala Thr Ser Ala Glu Pro Gly Ser Ser Asn His Thr Asn 375 380 385 atg ggt aat ctg gct gtg ttg tct cag gga aat aca agt tct tca agg 1256 Met Gly Asn Leu Ala Val Leu Ser Gln Gly Asn Thr Ser Ser Ser Arg 390 395 400 aga aaa aca aag tca aaa agc ata gct att gaa aat aag gaa cag aag 1304 Arg Lys Thr Lys Ser Lys Ser Ile Ala Ile Glu Asn Lys Glu Gln Lys 405 410 415 acc ggt aaa aca aat gaa tcg caa att tca aat aat ata aac atg cag 1352 Thr Gly Lys Thr Asn Glu Ser Gln Ile Ser Asn Asn Ile Asn Met Gln 420 425 430 435 agt tac tca gta gaa atg cct acc gtg tct tcc agt gga ggc ata att 1400 Ser Tyr Ser Val Glu Met Pro Thr Val Ser Ser Ser Gly Gly Ile Ile 440 445 450 ggc act gga ata gat gaa ctg cag aag agg gtg cca aaa ttg atc ttt 1448 Gly Thr Gly Ile Asp Glu Leu Gln Lys Arg Val Pro Lys Leu Ile Phe 455 460 465 aag aaa gga agc aga aag aat aca gat aaa aac tac ctt aac ttt gtg 1496 Lys Lys Gly Ser Arg Lys Asn Thr Asp Lys Asn Tyr Leu Asn Phe Val 470 475 480 tca cca tta cca gac ata gta gga cag aaa tcc ttg tct gga aaa cca 1544 Ser Pro Leu Pro Asp Ile Val Gly Gln Lys Ser Leu Ser Gly Lys Pro 485 490 495 agt ggc tca ctt ggc ata gta tca aat aat agt gtg gag acc att ggt 1592 Ser Gly Ser Leu Gly Ile Val Ser Asn Asn Ser Val Glu Thr Ile Gly 500 505 510 515 ctt ctc caa agt aca agt ggc aaa caa ggt cag ata agt agt aat tat 1640 Leu Leu Gln Ser Thr Ser Gly Lys Gln Gly Gln Ile Ser Ser Asn Tyr 520 525 530 gat gat gcc atg cag ttt tca aag aaa aga aga tat tta cca act gcc 1688 Asp Asp Ala Met Gln Phe Ser Lys Lys Arg Arg Tyr Leu Pro Thr Ala 535 540 545 agc agc aac agt gcc ttt tct ata aac gta gga cac atg gtc tcc caa 1736 Ser Ser Asn Ser Ala Phe Ser Ile Asn Val Gly His Met Val Ser Gln 550 555 560 cag tct gtc att cag tct gca ggt gtc agt gtt ttg gac aat gag gca 1784 Gln Ser Val Ile Gln Ser Ala Gly Val Ser Val Leu Asp Asn Glu Ala 565 570 575 cca ttg tca ctt att gac tcc tca gct cta aat gct gaa att aaa tct 1832 Pro Leu Ser Leu Ile Asp Ser Ser Ala Leu Asn Ala Glu Ile Lys Ser 580 585 590 595 tgt cat gac aag tct gga att cct gat gag gtt tta caa agt att ttg 1880 Cys His Asp Lys Ser Gly Ile Pro Asp Glu Val Leu Gln Ser Ile Leu 600 605 610 gat caa tac tcc aac aaa tca gaa agc cag aaa gag gat cct ttc aat 1928 Asp Gln Tyr Ser Asn Lys Ser Glu Ser Gln Lys Glu Asp Pro Phe Asn 615 620 625 att gca gaa cca cga gtg gat tta cac acc tca gga gaa cac tca gaa 1976 Ile Ala Glu Pro Arg Val Asp Leu His Thr Ser Gly Glu His Ser Glu 630 635 640 ttg gtt caa gaa gaa aat ttg agc cca ggc acc caa aca cct tca aat 2024 Leu Val Gln Glu Glu Asn Leu Ser Pro Gly Thr Gln Thr Pro Ser Asn 645 650 655 gat aaa gca agt atg ttg caa gaa tac tcc aaa tac ctc caa cag gct 2072 Asp Lys Ala Ser Met Leu Gln Glu Tyr Ser Lys Tyr Leu Gln Gln Ala 660 665 670 675 ttt gaa aaa tcc act aat gca agt ttt act ctt gga cac ggt ttc caa 2120 Phe Glu Lys Ser Thr Asn Ala Ser Phe Thr Leu Gly His Gly Phe Gln 680 685 690 ttt gtc agt ttg tct tca cct ctc cac aac cac act ttg ttt cca gaa 2168 Phe Val Ser Leu Ser Ser Pro Leu His Asn His Thr Leu Phe Pro Glu 695 700 705 aaa caa ata tac act acg tct cct ttg gag tgt ggt ttc ggc caa tct 2216 Lys Gln Ile Tyr Thr Thr Ser Pro Leu Glu Cys Gly Phe Gly Gln Ser 710 715 720 gtt acc tca gtg ttg cca tct tca ttg cca aag cct cct ttt ggg atg 2264 Val Thr Ser Val Leu Pro Ser Ser Leu Pro Lys Pro Pro Phe Gly Met 725 730 735 ttg ttt gga tct cag cca ggt ctt tat ttg tct gct ttg gat gct aca 2312 Leu Phe Gly Ser Gln Pro Gly Leu Tyr Leu Ser Ala Leu Asp Ala Thr 740 745 750 755 cat cag cag ttg aca cct tcc cag gag ctg gat gat ctg ata gat tct 2360 His Gln Gln Leu Thr Pro Ser Gln Glu Leu Asp Asp Leu Ile Asp Ser 760 765 770 cag aag aac tta gag act tca tca gcc ttc cag tcc tca tct cag aaa 2408 Gln Lys Asn Leu Glu Thr Ser Ser Ala Phe Gln Ser Ser Ser Gln Lys 775 780 785 ttg act agc cag aag gaa cag aaa aac tta gag tct tca aca ggc ttt 2456 Leu Thr Ser Gln Lys Glu Gln Lys Asn Leu Glu Ser Ser Thr Gly Phe 790 795 800 cag att cca tct cag gag tta gct agc cag ata gat cct cag aaa gac 2504 Gln Ile Pro Ser Gln Glu Leu Ala Ser Gln Ile Asp Pro Gln Lys Asp 805 810 815 ata gag cct aga aca acg tat cag att gag aac ttt gca caa gcg ttt 2552 Ile Glu Pro Arg Thr Thr Tyr Gln Ile Glu Asn Phe Ala Gln Ala Phe 820 825 830 835 ggt tct cag ttt aag tcg ggc agc agg gtg cca atg acc ttt atc act 2600 Gly Ser Gln Phe Lys Ser Gly Ser Arg Val Pro Met Thr Phe Ile Thr 840 845 850 aac tct aat gga gaa gtg gac cat aga gta agg act tca gtg tca gat 2648 Asn Ser Asn Gly Glu Val Asp His Arg Val Arg Thr Ser Val Ser Asp 855 860 865 ttc tca ggg tat aca aat atg atg tct gat gta agt gag cca tgt agt 2696 Phe Ser Gly Tyr Thr Asn Met Met Ser Asp Val Ser Glu Pro Cys Ser 870 875 880 aca aga gta aag aca ccc acc agc cag agt tac agg taaggtccca 2742 Thr Arg Val Lys Thr Pro Thr Ser Gln Ser Tyr Arg 885 890 895 aaagtggcca ggctggaggt cttctaatgt aattttgttt tattttgaga acactgccat 2802 tggaatgttt ctacacgatc ctattaagaa taatgtgatg ccctttcaat gcaacttttc 2862 atatttagtt tattttgtta gcgtgatttt agctctgttt gtattatgat ttttaatcaa 2922 aatcaataga ttaaaaatag tttgacattc aaagtgacaa tgtttagcaa tcaaatttac 2982 atgtatagat cgtcagggaa tagcccaaat gttttaaacg caaaaaaaaa aacaaaaaa 3041 <210> 94 <211> 3060 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb044-2 <220> <221> CDS <222> (69)..(2753) <400> 94 cggcgttatt tttattaact ggaggcgacg gcggctgcgg cggcggcggg acccgcctcc 60 tccggggt atg aaa atc ggc agt ggg ttc ctg agt ggc ggc gga ggt acc 110 Met Lys Ile Gly Ser Gly Phe Leu Ser Gly Gly Gly Gly Thr 1 5 10 ggc agt agc ggt ggt agc ggc tcc ggc ggc ggt ggt agt ggc ggc ggc 158 Gly Ser Ser Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 15 20 25 30 ggc ggc ggc ggc agc agc ggc agg agg gca gag atg gaa ccc acc ttt 206 Gly Gly Gly Gly Ser Ser Gly Arg Arg Ala Glu Met Glu Pro Thr Phe 35 40 45 ccc cag ggt atg gtt atg ttc aac cac cgt ctt ccc ccg gtc acc agc 254 Pro Gln Gly Met Val Met Phe Asn His Arg Leu Pro Pro Val Thr Ser 50 55 60 ttc acc cgg ccg gcg ggg tcg gcc gcc cct ccc ccg caa tgc gtg tta 302 Phe Thr Arg Pro Ala Gly Ser Ala Ala Pro Pro Pro Gln Cys Val Leu 65 70 75 tcc tcc tct acc tcc gca gcc ccg gcc gct gag ccc ccc cct ccg cca 350 Ser Ser Ser Thr Ser Ala Ala Pro Ala Ala Glu Pro Pro Pro Pro Pro 80 85 90 gcc ccg gac atg act ttc aag aag gag ccg gcg gcg tca gcc gcg gcc 398 Ala Pro Asp Met Thr Phe Lys Lys Glu Pro Ala Ala Ser Ala Ala Ala 95 100 105 110 ttc ccc tcg cag agg acc tcc tgg ggg ttc ttg cag tct ttg ggt agc 446 Phe Pro Ser Gln Arg Thr Ser Trp Gly Phe Leu Gln Ser Leu Gly Ser 115 120 125 atc aaa cag gag aaa ccc gcg gat cct gag gag cag cag tcc cac cac 494 Ile Lys Gln Glu Lys Pro Ala Asp Pro Glu Glu Gln Gln Ser His His 130 135 140 cac cat cac cac cac cac tat ggg ggg ctg ttc gct gga gct gaa gag 542 His His His His His His Tyr Gly Gly Leu Phe Ala Gly Ala Glu Glu 145 150 155 agg tct cca ggc cta gga ggc ggt gaa ggg ggg agt cac ggc gtc atc 590 Arg Ser Pro Gly Leu Gly Gly Gly Glu Gly Gly Ser His Gly Val Ile 160 165 170 cag gac ctc agt att ctc cac cag cat gtc cag cag caa cca gcc cag 638 Gln Asp Leu Ser Ile Leu His Gln His Val Gln Gln Gln Pro Ala Gln 175 180 185 190 cac cac cgt gac gta tta ctc agc agc agt agc agg act gat gac cac 686 His His Arg Asp Val Leu Leu Ser Ser Ser Ser Arg Thr Asp Asp His 195 200 205 cat ggc act gag gag cca aag cag gac act aat gtc aaa aag gca aaa 734 His Gly Thr Glu Glu Pro Lys Gln Asp Thr Asn Val Lys Lys Ala Lys 210 215 220 agg cca aag cca gaa tct cag gga atc aaa gcc aag agg aag cca agt 782 Arg Pro Lys Pro Glu Ser Gln Gly Ile Lys Ala Lys Arg Lys Pro Ser 225 230 235 gca tct tcc aaa cct tct ttg gtt gga gat gga gaa ggt gcc atc ctc 830 Ala Ser Ser Lys Pro Ser Leu Val Gly Asp Gly Glu Gly Ala Ile Leu 240 245 250 tcc cca agt cag aaa cct cat atc tgt gat cac tgt agt gct gct ttc 878 Ser Pro Ser Gln Lys Pro His Ile Cys Asp His Cys Ser Ala Ala Phe 255 260 265 270 cga agc tcc tat cac ctg cgg aga cat gtc ctc att cat aca gga gaa 926 Arg Ser Ser Tyr His Leu Arg Arg His Val Leu Ile His Thr Gly Glu 275 280 285 aga cct ttc cag tgc agc cag tgt agt atg ggt ttc att cag aaa tac 974 Arg Pro Phe Gln Cys Ser Gln Cys Ser Met Gly Phe Ile Gln Lys Tyr 290 295 300 cta cta cag aga cat gag aaa att cat agt aga gag aag cca ttt gga 1022 Leu Leu Gln Arg His Glu Lys Ile His Ser Arg Glu Lys Pro Phe Gly 305 310 315 tgt gat cag tgc agc atg aag ttt att cag aag tac cat atg gag aga 1070 Cys Asp Gln Cys Ser Met Lys Phe Ile Gln Lys Tyr His Met Glu Arg 320 325 330 cac aag agg aca cat agt gga gaa aag cca tat aag tgt gac act tgc 1118 His Lys Arg Thr His Ser Gly Glu Lys Pro Tyr Lys Cys Asp Thr Cys 335 340 345 350 caa cag tat ttt tca agg act gat aga ttg ttg aag cac agg cgc aca 1166 Gln Gln Tyr Phe Ser Arg Thr Asp Arg Leu Leu Lys His Arg Arg Thr 355 360 365 tgt ggt gaa gtc ata gtt aaa gga gcc act agt gca gaa cct ggg tca 1214 Cys Gly Glu Val Ile Val Lys Gly Ala Thr Ser Ala Glu Pro Gly Ser 370 375 380 tca aac cat acc aat atg ggt aat ctg gct gtg ttg tct cag gga aat 1262 Ser Asn His Thr Asn Met Gly Asn Leu Ala Val Leu Ser Gln Gly Asn 385 390 395 aca agt tct tca agg aga aaa aca aag tca aaa agc ata gct att gaa 1310 Thr Ser Ser Ser Arg Arg Lys Thr Lys Ser Lys Ser Ile Ala Ile Glu 400 405 410 aat aag gaa cag aag acc ggt aaa aca aat gaa tcg caa att tca aat 1358 Asn Lys Glu Gln Lys Thr Gly Lys Thr Asn Glu Ser Gln Ile Ser Asn 415 420 425 430 aat ata aac atg cag agt tac tca gta gaa atg cct acc gtg tct tcc 1406 Asn Ile Asn Met Gln Ser Tyr Ser Val Glu Met Pro Thr Val Ser Ser 435 440 445 agt gga ggc ata att ggc act gga ata gat gaa ctg cag aag agg gtg 1454 Ser Gly Gly Ile Ile Gly Thr Gly Ile Asp Glu Leu Gln Lys Arg Val 450 455 460 cca aaa ttg atc ttt aag aaa gga agc aga aag aat aca gat aaa aac 1502 Pro Lys Leu Ile Phe Lys Lys Gly Ser Arg Lys Asn Thr Asp Lys Asn 465 470 475 tac ctt aac ttt gtg tca cca tta cca gac ata gta gga cag aaa tcc 1550 Tyr Leu Asn Phe Val Ser Pro Leu Pro Asp Ile Val Gly Gln Lys Ser 480 485 490 ttg tct gga aaa cca agt ggc tca ctt ggc ata gta tca aat aat agt 1598 Leu Ser Gly Lys Pro Ser Gly Ser Leu Gly Ile Val Ser Asn Asn Ser 495 500 505 510 gtg gag acc att ggt ctt ctc caa agt aca agt ggc aaa caa ggt cag 1646 Val Glu Thr Ile Gly Leu Leu Gln Ser Thr Ser Gly Lys Gln Gly Gln 515 520 525 ata agt agt aat tat gat gat gcc atg cag ttt tca aag aaa aga aga 1694 Ile Ser Ser Asn Tyr Asp Asp Ala Met Gln Phe Ser Lys Lys Arg Arg 530 535 540 tat tta cca act gcc agc agc aac agt gcc ttt tct ata aac gta gga 1742 Tyr Leu Pro Thr Ala Ser Ser Asn Ser Ala Phe Ser Ile Asn Val Gly 545 550 555 cac atg gtc tcc caa cag tct gtc att cag tct gca ggt gtc agt gtt 1790 His Met Val Ser Gln Gln Ser Val Ile Gln Ser Ala Gly Val Ser Val 560 565 570 ttg gac aat gag gca cca ttg tca ctt att gac tcc tca gct cta aat 1838 Leu Asp Asn Glu Ala Pro Leu Ser Leu Ile Asp Ser Ser Ala Leu Asn 575 580 585 590 gct gaa att aaa tct tgt cat gac aag tct gga att cct gat gag gtt 1886 Ala Glu Ile Lys Ser Cys His Asp Lys Ser Gly Ile Pro Asp Glu Val 595 600 605 tta caa agt att ttg gat caa tac tcc aac aaa tca gaa agc cag aaa 1934 Leu Gln Ser Ile Leu Asp Gln Tyr Ser Asn Lys Ser Glu Ser Gln Lys 610 615 620 gag gat cct ttc aat att gca gaa cca cga gtg gat tta cac acc tca 1982 Glu Asp Pro Phe Asn Ile Ala Glu Pro Arg Val Asp Leu His Thr Ser 625 630 635 gga gaa cac tca gaa ttg gtt caa gaa gaa aat ttg agc cca ggc acc 2030 Gly Glu His Ser Glu Leu Val Gln Glu Glu Asn Leu Ser Pro Gly Thr 640 645 650 caa aca cct tca aat gat aaa gca agt atg ttg caa gaa tac tcc aaa 2078 Gln Thr Pro Ser Asn Asp Lys Ala Ser Met Leu Gln Glu Tyr Ser Lys 655 660 665 670 tac ctc caa cag gct ttt gaa aaa tcc act aat gca agt ttt act ctt 2126 Tyr Leu Gln Gln Ala Phe Glu Lys Ser Thr Asn Ala Ser Phe Thr Leu 675 680 685 gga cac ggt ttc caa ttt gtc agt ttg tct tca cct ctc cac aac cac 2174 Gly His Gly Phe Gln Phe Val Ser Leu Ser Ser Pro Leu His Asn His 690 695 700 act ttg ttt cca gaa aaa caa ata tac act acg tct cct ttg gag tgt 2222 Thr Leu Phe Pro Glu Lys Gln Ile Tyr Thr Thr Ser Pro Leu Glu Cys 705 710 715 ggt ttc ggc caa tct gtt acc tca gtg ttg cca tct tca ttg cca aag 2270 Gly Phe Gly Gln Ser Val Thr Ser Val Leu Pro Ser Ser Leu Pro Lys 720 725 730 cct cct ttt ggg atg ttg ttt gga tct cag cca ggt ctt tat ttg tct 2318 Pro Pro Phe Gly Met Leu Phe Gly Ser Gln Pro Gly Leu Tyr Leu Ser 735 740 745 750 gct ttg gat gct aca cat cag cag ttg aca cct tcc cag gag ctg gat 2366 Ala Leu Asp Ala Thr His Gln Gln Leu Thr Pro Ser Gln Glu Leu Asp 755 760 765 gat ctg ata gat tct cag aag aac tta gag act tca tca gcc ttc cag 2414 Asp Leu Ile Asp Ser Gln Lys Asn Leu Glu Thr Ser Ser Ala Phe Gln 770 775 780 tcc tca tct cag aaa ttg act agc cag aag gaa cag aaa aac tta gag 2462 Ser Ser Ser Gln Lys Leu Thr Ser Gln Lys Glu Gln Lys Asn Leu Glu 785 790 795 tct tca aca ggc ttt cag att cca tct cag gag tta gct agc cag ata 2510 Ser Ser Thr Gly Phe Gln Ile Pro Ser Gln Glu Leu Ala Ser Gln Ile 800 805 810 gat cct cag aaa gac ata gag cct aga aca acg tat cag att gag aac 2558 Asp Pro Gln Lys Asp Ile Glu Pro Arg Thr Thr Tyr Gln Ile Glu Asn 815 820 825 830 ttt gca caa gcg ttt ggt tct cag ttt aag tcg ggc agc agg gtg cca 2606 Phe Ala Gln Ala Phe Gly Ser Gln Phe Lys Ser Gly Ser Arg Val Pro 835 840 845 atg acc ttt atc act aac tct aat gga gaa gtg gac cat aga gta agg 2654 Met Thr Phe Ile Thr Asn Ser Asn Gly Glu Val Asp His Arg Val Arg 850 855 860 act tca gtg tca gat ttc tca ggg tat aca aat atg atg tct gat gta 2702 Thr Ser Val Ser Asp Phe Ser Gly Tyr Thr Asn Met Met Ser Asp Val 865 870 875 agt gag cca tgt agt aca aga gta aag aca ccc acc agc cag agt tac 2750 Ser Glu Pro Cys Ser Thr Arg Val Lys Thr Pro Thr Ser Gln Ser Tyr 880 885 890 agg taaggtccca aaagtggcca ggctggaggt cttctaatgt aattttgttt 2803 Arg 895 tattttgaga acactgccat tggaatgttt ctacacgatc ctattaagaa taatgtgatg 2863 ccctttcaat gcaacttttc atatttagtt tattttgtta gcgtgatttt agctctgttt 2923 gtattatgat ttttaatcaa aatcaataga ttaaaaatag tttgacattc aaagtgacaa 2983 tgtttagcaa tcaaatttac atgtatagat cgtcagggaa tagcccaaat gttttaaacg 3043 caaaaaaaaa aacaaaa 3060 <210> 95 <211> 984 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb060-1 <400> 95 attggcgcag gctggcgctg ggaacccccg cgccggttac ccctttcctc ggtggtagag 60 gccgggcggg gcagcgcatg cgcgtggcgg gcaggcggca ggaggcgggt gggtcaaggt 120 aactctgggc tacagagtcc ttgctggggg ttcggggagc gcttggaccc cggcttctgg 180 gacgcgtcag gagaagggag cactggcttt gctttcatca ggccaaagat gcctttcttt 240 gggaatacgt tcagtccgaa gaagacacct cctcggaagt cggcatctct ctccaacctg 300 cattctttgg atcgatcaac ccgggaggtg gagctgggct tggaatacgg atccccgact 360 atgaacctgg cagggcaaag cctgaagttt gaaaatggcc agtggatagc agagacaggg 420 gttagtggcg gtgtggaccg gagggaggtt cagcgccttc gcaggcggaa ccagcagttg 480 gaggaagaga acaatctctt gcggctgaaa gtggacatct tattagacat gctttcagag 540 tccactgccg aatcccactt aatggagaag gaactggatg aactgaggat cagccggaag 600 agaaaatgaa gaccccagag acatttattg gggagtagga tgtggctgag tgcttttttt 660 ttggccagac tagcggattc agtcctggaa gagagtatca tataatgaga cccacaggca 720 ctggcaccct tgggttggca atagaaggtg acatggaatg gagaaaacca agattccaga 780 tggggatagt aactagaagg tgcttcagat gcactgcctg cgggtgccag tctgaaaacc 840 agaccgcaca gaggcctggg gctgctgatg agcttttcgg tgctctccac acacaagctc 900 gcaaacacac atgtcccaga atagctctgt tgggttgtgt tgggagaagc ggctggagtt 960 cattctctca accccttatg ttgg 984 <210> 96 <211> 982 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb060-2 <400> 96 gcggcaggag gcgggtgggt caaggtaact ctgggctaca gagtccttgc tgggggttcg 60 gggagcgctt ggaccccggc ttctgggacg cgtcagagac gggccctgct aagttgccca 120 ggctggtctt gaactcctgt gctcaagtaa tcctccctcc ctggcctccc aaagtgctgg 180 gattacagga gaagggagca ctggctttgc tttcatcagg ccaaagatgc ctttctttgg 240 gaatacgttc agtccgaaga agacacctcc tcggaagtcg gcatctctct ccaacctgca 300 ttctttggat cgatcaaccc gggaggtgga gctgggcttg gaatacggat ccccgactat 360 gaacctggca gggcaaagcc tgaagtttga aaatggccag tggatagcag agacaggggt 420 tagtggcggt gtggaccgga gggaggttca gcgccttcgc aggcggaacc agcagttgga 480 ggaagagaac aatctcttgc ggctgaaagt ggacatctta ttagacatgc tttcagagtc 540 cactgccgaa tcccacttaa tggagaagga actggatgaa ctgaggatca gccggaagag 600 aaaatgaaga ccccagagac atttattggg gagtaggatg tggctgagtg cttttttttt 660 ggccagacta gcggattcag tcctggaaga gagtatcata taatgagacc cacaggcact 720 ggcacccttg ggttggcaat agaaggtgac atggaatgga gaaaaccaag attccagatg 780 gggatagtaa ctagaaggtg cttcagatgc actgcctgcg ggtgccagtc tgaaaaccag 840 accgcacaga ggcctggggc tgctgatgag cttttcggtg ctctccacac acaagctcgc 900 aaacacacat gtcccagaat agctctgttg ggttgtgttg ggagaagcgg ctggagttca 960 ttctctcaac cccttatgtt gg 982 <210> 97 <211> 1101 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb066 <220> <221> CDS <222> (1)..(1101) <400> 97 aaa aat tac aaa aat gcc agt gga gtt gtg aac tct tca cct cga agt 48 Lys Asn Tyr Lys Asn Ala Ser Gly Val Val Asn Ser Ser Pro Arg Ser 1 5 10 15 cat agc gcc aca aat ggg agc att cct tcc tca tct agt aaa aat gag 96 His Ser Ala Thr Asn Gly Ser Ile Pro Ser Ser Ser Ser Lys Asn Glu 20 25 30 aag aag cag aaa tgc act agc aag agc cca agt aca cac aag gac tta 144 Lys Lys Gln Lys Cys Thr Ser Lys Ser Pro Ser Thr His Lys Asp Leu 35 40 45 atg gaa aac tgt att cct aat aac cag cta agc aaa cca gac gca ctg 192 Met Glu Asn Cys Ile Pro Asn Asn Gln Leu Ser Lys Pro Asp Ala Leu 50 55 60 gtc agg ctg gaa caa gac att aaa aag tta aag gct gac ctg caa gcc 240 Val Arg Leu Glu Gln Asp Ile Lys Lys Leu Lys Ala Asp Leu Gln Ala 65 70 75 80 agc aga caa gtg gaa caa gag ctc cgc agt cag atc agc tcc ctt tcg 288 Ser Arg Gln Val Glu Gln Glu Leu Arg Ser Gln Ile Ser Ser Leu Ser 85 90 95 agc acc gag cga ggg atc cgc tca gaa atg ggc cag ctt cgg cag gag 336 Ser Thr Glu Arg Gly Ile Arg Ser Glu Met Gly Gln Leu Arg Gln Glu 100 105 110 aac gag ctg ctg cag aac aag tta cat aat gct gtg caa atg aag caa 384 Asn Glu Leu Leu Gln Asn Lys Leu His Asn Ala Val Gln Met Lys Gln 115 120 125 aaa gac aag cag aat atc agc cag ttg gag aaa aag cta aaa gct gag 432 Lys Asp Lys Gln Asn Ile Ser Gln Leu Glu Lys Lys Leu Lys Ala Glu 130 135 140 cag gaa gcc cga agt ttt gta gag aaa cag tta atg gaa gag aaa aag 480 Gln Glu Ala Arg Ser Phe Val Glu Lys Gln Leu Met Glu Glu Lys Lys 145 150 155 160 agg aag aag tta gaa gaa gcc act gct gcc cgg gct gtt gcg ttt gct 528 Arg Lys Lys Leu Glu Glu Ala Thr Ala Ala Arg Ala Val Ala Phe Ala 165 170 175 gct gca tct agg gga gaa tgc acc gaa acc tta cgg aat cgg atc aga 576 Ala Ala Ser Arg Gly Glu Cys Thr Glu Thr Leu Arg Asn Arg Ile Arg 180 185 190 gaa cta gaa gca gag ggc aag aag ctc acg atg gac atg aag gtg aaa 624 Glu Leu Glu Ala Glu Gly Lys Lys Leu Thr Met Asp Met Lys Val Lys 195 200 205 gaa gac caa atc aga gaa cta gaa cta aaa gtc cag gag ctt cgg aaa 672 Glu Asp Gln Ile Arg Glu Leu Glu Leu Lys Val Gln Glu Leu Arg Lys 210 215 220 tat aag gaa aat gag aag gac act gag gtg tta atg tca gcc ctc tca 720 Tyr Lys Glu Asn Glu Lys Asp Thr Glu Val Leu Met Ser Ala Leu Ser 225 230 235 240 gcc atg caa gac aaa aca cag cac ctg gag aac agc tta agt gca gag 768 Ala Met Gln Asp Lys Thr Gln His Leu Glu Asn Ser Leu Ser Ala Glu 245 250 255 acg aga atc aag ctg gac ctg ttc tcc gca ctg ggc gat gca aag cgg 816 Thr Arg Ile Lys Leu Asp Leu Phe Ser Ala Leu Gly Asp Ala Lys Arg 260 265 270 cag ctc gag att gcc caa gga caa atc ctt cag aaa gat cag gga aat 864 Gln Leu Glu Ile Ala Gln Gly Gln Ile Leu Gln Lys Asp Gln Gly Asn 275 280 285 caa gga cct aaa aca gaa gat agc cga agt cat ggc cgt cat gcc cag 912 Gln Gly Pro Lys Thr Glu Asp Ser Arg Ser His Gly Arg His Ala Gln 290 295 300 cat aac ata cag tgc cgc cac cag ccc cct gag ccc tgt ttc ccc cca 960 His Asn Ile Gln Cys Arg His Gln Pro Pro Glu Pro Cys Phe Pro Pro 305 310 315 320 cta ctc ttc caa att tgt gga gac cag ccc ctc tgg act ttg acc cca 1008 Leu Leu Phe Gln Ile Cys Gly Asp Gln Pro Leu Trp Thr Leu Thr Pro 325 330 335 atg gcc tct gtt tac cag ccc ctg aag aaa ttg aag gcc agc ctg tgt 1056 Met Ala Ser Val Tyr Gln Pro Leu Lys Lys Leu Lys Ala Ser Leu Cys 340 345 350 gtt gtg ccc aaa aaa ttt ggg tta ccg gga aag gca ttg caa agg 1101 Val Val Pro Lys Lys Phe Gly Leu Pro Gly Lys Ala Leu Gln Arg 355 360 365 <210> 98 <211> 2565 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb101-1 <220> <221> CDS <222> (474)..(2117) <400> 98 cgcccccacc gccacccaac caagcatcac ccattccagc acttccggcc tgacccctgt 60 ccagcgcctt tcctttctcc agcttctgct ctgccccaag agtggtcgcc ttcgtggcag 120 ggcacgactc tctcccagct cgggtcgccg cccacattag tgtggggccc tgcggcctag 180 cgtccctcac cagaggcctc cccttgccta gctggaccgc cgagggacat cgacgagtat 240 cctcctcctg ctgtccccgg cttcgcctgc cgcccctaac cggccagtca agatggccgc 300 cgctgggtga ggcaagctgg cgcgccgcgg gggcgtctgg gagttgtagt tcgggacggc 360 gggctgacgc acttcgccgt cggccgacgg gcgccattgt gcggcgcgcg ccggagtatc 420 ctgagcccca gctggaccct aaattggatc ctcttcctgc tgagagtccc cta atg 476 Met 1 aac att gag gtt gtt gag gtc ctc aca ctg aac cag gag gtg gct ggt 524 Asn Ile Glu Val Val Glu Val Leu Thr Leu Asn Gln Glu Val Ala Gly 5 10 15 ccc cgg aat gcc cag atc cag gcc cta tat gct gaa gat gga agc ctg 572 Pro Arg Asn Ala Gln Ile Gln Ala Leu Tyr Ala Glu Asp Gly Ser Leu 20 25 30 agt gca gat gcc ccc agt gag cag atc caa cag cag ggc aag cat cca 620 Ser Ala Asp Ala Pro Ser Glu Gln Ile Gln Gln Gln Gly Lys His Pro 35 40 45 ggt gac cct gag gcc gcg cgc cag agg ttc cgg cag ttc cgt tat aag 668 Gly Asp Pro Glu Ala Ala Arg Gln Arg Phe Arg Gln Phe Arg Tyr Lys 50 55 60 65 gac atg aca ggt ccc cgg gag gcc ctg gac cag ctc cga gag ctg tgt 716 Asp Met Thr Gly Pro Arg Glu Ala Leu Asp Gln Leu Arg Glu Leu Cys 70 75 80 cac cag tgg cta cag cct aag gca cgc tcc aag gag cag atc ctg gag 764 His Gln Trp Leu Gln Pro Lys Ala Arg Ser Lys Glu Gln Ile Leu Glu 85 90 95 ctg ctg gtg ctg gag cag ttc cta ggt gca ctg cct gtg aag ctc cgg 812 Leu Leu Val Leu Glu Gln Phe Leu Gly Ala Leu Pro Val Lys Leu Arg 100 105 110 aca tgg gtg gaa tcg cag cac cca gag aac tgc caa gag gtg gtg gcc 860 Thr Trp Val Glu Ser Gln His Pro Glu Asn Cys Gln Glu Val Val Ala 115 120 125 ctg gta gag ggt gtg acc tgg atg tct gag gag gaa gta ctt cct gca 908 Leu Val Glu Gly Val Thr Trp Met Ser Glu Glu Glu Val Leu Pro Ala 130 135 140 145 gga caa cct gcc gag ggc acc acc tgc tgc ctc gag gtc act gcc cag 956 Gly Gln Pro Ala Glu Gly Thr Thr Cys Cys Leu Glu Val Thr Ala Gln 150 155 160 cag gag gag aag cag gag gat gca gcc atc tgc cca gtg aca gtg ctc 1004 Gln Glu Glu Lys Gln Glu Asp Ala Ala Ile Cys Pro Val Thr Val Leu 165 170 175 cct gag gag cca gtg acc ttc cag gat gtg gct gtg gac ttc agc cgg 1052 Pro Glu Glu Pro Val Thr Phe Gln Asp Val Ala Val Asp Phe Ser Arg 180 185 190 gag gag tgg ggg ctg ctg ggc ccg aca cag agg acc gag tac cgc gat 1100 Glu Glu Trp Gly Leu Leu Gly Pro Thr Gln Arg Thr Glu Tyr Arg Asp 195 200 205 gtg atg ctg gag acc ttt ggg cac ctg gtc tct gtg ggg tgg gag act 1148 Val Met Leu Glu Thr Phe Gly His Leu Val Ser Val Gly Trp Glu Thr 210 215 220 225 aca ctg gaa aat aaa gag tta gct cca aat tct gac att cct gag gaa 1196 Thr Leu Glu Asn Lys Glu Leu Ala Pro Asn Ser Asp Ile Pro Glu Glu 230 235 240 gaa cca gcc ccc agc ctg aaa gta caa gaa tcc tca agg gat tgt gcc 1244 Glu Pro Ala Pro Ser Leu Lys Val Gln Glu Ser Ser Arg Asp Cys Ala 245 250 255 ttg tcc tct aca tta gaa gat acc ttg cag ggt ggg gtc cag gaa gtc 1292 Leu Ser Ser Thr Leu Glu Asp Thr Leu Gln Gly Gly Val Gln Glu Val 260 265 270 caa gac aca gtg ttg aag cag atg gag tct gct cag gaa aaa gac ctt 1340 Gln Asp Thr Val Leu Lys Gln Met Glu Ser Ala Gln Glu Lys Asp Leu 275 280 285 cct cag aag aag cac ttt gac aac cgt gag tcc cag gca aac agt ggt 1388 Pro Gln Lys Lys His Phe Asp Asn Arg Glu Ser Gln Ala Asn Ser Gly 290 295 300 305 gct ctt gac aca aac caa gtt tcg ctc cag aaa att gac aac cct gag 1436 Ala Leu Asp Thr Asn Gln Val Ser Leu Gln Lys Ile Asp Asn Pro Glu 310 315 320 tcc cag gca aac agt ggc gct ctt gac aca aac caa gtt ttg ctc cac 1484 Ser Gln Ala Asn Ser Gly Ala Leu Asp Thr Asn Gln Val Leu Leu His 325 330 335 aaa att cct cct aga aaa cga ttg cgc aaa cgt gac tca caa gtt aaa 1532 Lys Ile Pro Pro Arg Lys Arg Leu Arg Lys Arg Asp Ser Gln Val Lys 340 345 350 agt atg aaa cat aat tca cgt gta aaa att cat cag aag agc tgt gaa 1580 Ser Met Lys His Asn Ser Arg Val Lys Ile His Gln Lys Ser Cys Glu 355 360 365 agg caa aag gcc aag gaa ggc aat ggt tgt agg aaa acc ttc agt cgg 1628 Arg Gln Lys Ala Lys Glu Gly Asn Gly Cys Arg Lys Thr Phe Ser Arg 370 375 380 385 agt act aaa cag att acg ttt ata aga att cac aag ggg agc caa gtt 1676 Ser Thr Lys Gln Ile Thr Phe Ile Arg Ile His Lys Gly Ser Gln Val 390 395 400 tgc cga tgc agt gaa tgt ggt aaa ata ttc cgg aac cca aga tac ttt 1724 Cys Arg Cys Ser Glu Cys Gly Lys Ile Phe Arg Asn Pro Arg Tyr Phe 405 410 415 tct gtg cat aag aaa atc cat acc gga gag agg ccc tat gtg tgt caa 1772 Ser Val His Lys Lys Ile His Thr Gly Glu Arg Pro Tyr Val Cys Gln 420 425 430 gac tgt ggg aaa gga ttt gtt cag agc tct tcc ctc aca cag cat cag 1820 Asp Cys Gly Lys Gly Phe Val Gln Ser Ser Ser Leu Thr Gln His Gln 435 440 445 aga gtt cat tct gga gag aga cca ttt gaa tgt cag gag tgt ggg agg 1868 Arg Val His Ser Gly Glu Arg Pro Phe Glu Cys Gln Glu Cys Gly Arg 450 455 460 465 acc ttc aat gat cgc tca gcc atc tcc cag cac ctg agg act cac act 1916 Thr Phe Asn Asp Arg Ser Ala Ile Ser Gln His Leu Arg Thr His Thr 470 475 480 ggc gct aag ccc tac aag tgt cag gac tgt gga aaa gcc ttc cgc cag 1964 Gly Ala Lys Pro Tyr Lys Cys Gln Asp Cys Gly Lys Ala Phe Arg Gln 485 490 495 agc tcc cac ctc atc aga cat cag agg act cac acc ggg gag cgc cca 2012 Ser Ser His Leu Ile Arg His Gln Arg Thr His Thr Gly Glu Arg Pro 500 505 510 tat gca tgc aac aaa tgt gga aag gcc ttc acc cag agc tca cac ctt 2060 Tyr Ala Cys Asn Lys Cys Gly Lys Ala Phe Thr Gln Ser Ser His Leu 515 520 525 att tgg cac cag aga acc cac aat agg aca aag cga aag aag aaa cag 2108 Ile Trp His Gln Arg Thr His Asn Arg Thr Lys Arg Lys Lys Lys Gln 530 535 540 545 cct acc tca tagctctcaa gccagttgaa gaaaccttgc cttttcagct 2157 Pro Thr Ser tgaccctgca atataacatg cacaggcctg cttgtgaatc aggactgaat gtgaaaggga 2217 agtattgagt gaggacattc ccaaaaccaa aggacaactg aggagactgc ccagcacata 2277 atgaataaat aagaaaatga gtgaggagtt attaacatca tttggaaaaa agatttccca 2337 ttcacttgat attgtttgtt cactcattta gtcattaaaa gtgagattaa taaaatctga 2397 aaatgttata taataacttt aaaaagccag gtaattaata atctgcactg atattacatc 2457 cacagtacca cagtatttat gtgtatgaat taaggattaa aagataatgt ggataaataa 2517 actattgatc tatgtctgtg taaaaaaaaa aaaaaaaaaa aaaaaaaa 2565 <210> 99 <211> 2694 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb101-2 <220> <221> CDS <222> (402)..(2264) <400> 99 gtccagcgcc tttcctttct ccagcttctg ctctgcccca agagtggtcg ccttcgtggc 60 agggcacgac tctctcccag ctcgggtcgc cgcccacatt agtgtggggc cctgcggcct 120 agcgtccctc accagaggcc tccccttgcc tagctggacc gccgagggac atcgacgagt 180 atcctcctcc tgctgtcccc ggcttcgcct gccgccccta accggccagt caagatggcc 240 gccgctgggt gaggcaagct ggcgcgccgc gggggcgtct gggagttgta gttcgggacg 300 gcgggctgac gcacttcgcc gccggccgac gggcgccatt gtgcggcgcg cgccgggact 360 ctgcccactt ccaccagaga cacattgaga aggaggaaac t atg gcc tcc agg ctt 416 Met Ala Ser Arg Leu 1 5 ccg acg gcc tgg tcc tgt gaa cca gtg acc ttt gaa gat gta aca ctg 464 Pro Thr Ala Trp Ser Cys Glu Pro Val Thr Phe Glu Asp Val Thr Leu 10 15 20 ggt ttt acc ccg gaa gag tgg gga ctg ctg gac ctc aaa cag aag tcc 512 Gly Phe Thr Pro Glu Glu Trp Gly Leu Leu Asp Leu Lys Gln Lys Ser 25 30 35 ctg tac agg gaa gtg atg ctg gag aac tac agg aac ctg gtc tca gtg 560 Leu Tyr Arg Glu Val Met Leu Glu Asn Tyr Arg Asn Leu Val Ser Val 40 45 50 gag tat cct gag ctc cag ctg gac cct aaa ttg gat cct ctt cct gct 608 Glu Tyr Pro Glu Leu Gln Leu Asp Pro Lys Leu Asp Pro Leu Pro Ala 55 60 65 gag agt ccc cta atg aac att gag gtt gtt gag gtc ctc aca ctg aac 656 Glu Ser Pro Leu Met Asn Ile Glu Val Val Glu Val Leu Thr Leu Asn 70 75 80 85 cag gag gtg gct ggt ccc cgg aat gcc cag atc cag gcc cta tat gct 704 Gln Glu Val Ala Gly Pro Arg Asn Ala Gln Ile Gln Ala Leu Tyr Ala 90 95 100 gaa gat gga agc ctg agt gca gat gcc ccc agt gag cag gtc caa cag 752 Glu Asp Gly Ser Leu Ser Ala Asp Ala Pro Ser Glu Gln Val Gln Gln 105 110 115 cag ggc aag cat cca ggt gac cct gag gcc gcg cgc cag agg ttc cgg 800 Gln Gly Lys His Pro Gly Asp Pro Glu Ala Ala Arg Gln Arg Phe Arg 120 125 130 cag ttc cgt tat aag gac atg aca ggt ccc cgg gag gcc ctg gac cag 848 Gln Phe Arg Tyr Lys Asp Met Thr Gly Pro Arg Glu Ala Leu Asp Gln 135 140 145 ctc cga gag ctg tgt cac cag tgg cta cag cct aag gca cgc tcc aag 896 Leu Arg Glu Leu Cys His Gln Trp Leu Gln Pro Lys Ala Arg Ser Lys 150 155 160 165 gag cag atc ctg gag ctg ctg gtg ctg gag cag ttc cta ggt gca ctg 944 Glu Gln Ile Leu Glu Leu Leu Val Leu Glu Gln Phe Leu Gly Ala Leu 170 175 180 cct gtg aag ctc cgg aca tgg gtg gaa tcg cag cac cca gag aac tgc 992 Pro Val Lys Leu Arg Thr Trp Val Glu Ser Gln His Pro Glu Asn Cys 185 190 195 caa gag gtg gtg gcc ctg gta gag ggt gtg acc tgg atg tct gag gag 1040 Gln Glu Val Val Ala Leu Val Glu Gly Val Thr Trp Met Ser Glu Glu 200 205 210 gaa gta ctt cct gca gga caa cct gcc gag ggc acc acc tgc tgc ctc 1088 Glu Val Leu Pro Ala Gly Gln Pro Ala Glu Gly Thr Thr Cys Cys Leu 215 220 225 gag gtc act gcc cag cag gag gag aag cag gag gat gca gcc atc tgc 1136 Glu Val Thr Ala Gln Gln Glu Glu Lys Gln Glu Asp Ala Ala Ile Cys 230 235 240 245 cca gtg aca gtg ctc cct gag gag cca gtg acc ttc cag gat gtg gct 1184 Pro Val Thr Val Leu Pro Glu Glu Pro Val Thr Phe Gln Asp Val Ala 250 255 260 gtg gac ttc agc cgg gag gag tgg ggg ctg ctg ggc ccg aca cag agg 1232 Val Asp Phe Ser Arg Glu Glu Trp Gly Leu Leu Gly Pro Thr Gln Arg 265 270 275 acc gag tac cgc gat gtg atg ctg gag acc ttt ggg cac ctg gtc tct 1280 Thr Glu Tyr Arg Asp Val Met Leu Glu Thr Phe Gly His Leu Val Ser 280 285 290 gtg ggg tgg gag act aca ctg gaa aat aaa gag tta gct cca aat tct 1328 Val Gly Trp Glu Thr Thr Leu Glu Asn Lys Glu Leu Ala Pro Asn Ser 295 300 305 gac att cct gag gaa gaa cca gcc ccc agc ctg aaa gta caa gaa tcc 1376 Asp Ile Pro Glu Glu Glu Pro Ala Pro Ser Leu Lys Val Gln Glu Ser 310 315 320 325 tca agg gat tgt gcc ttg tcc tct aca tta gaa gat acc ttg cag ggt 1424 Ser Arg Asp Cys Ala Leu Ser Ser Thr Leu Glu Asp Thr Leu Gln Gly 330 335 340 ggg gtc cag gaa gtc caa gac aca gtg ttg aag cag atg gag tct gct 1472 Gly Val Gln Glu Val Gln Asp Thr Val Leu Lys Gln Met Glu Ser Ala 345 350 355 cag gaa aaa gac ctt cct cag aag aag cac ttt gac aac cgt gag tcc 1520 Gln Glu Lys Asp Leu Pro Gln Lys Lys His Phe Asp Asn Arg Glu Ser 360 365 370 cag gca aac agt ggt gct ctt gac aca aac caa gtt tcg ctc cag aaa 1568 Gln Ala Asn Ser Gly Ala Leu Asp Thr Asn Gln Val Ser Leu Gln Lys 375 380 385 att gac aac cct gag tcc cag gca aac agt ggc gct ctt gac aca aac 1616 Ile Asp Asn Pro Glu Ser Gln Ala Asn Ser Gly Ala Leu Asp Thr Asn 390 395 400 405 caa gtt ttg ctc cac aaa att cct cct aga aaa cga ttg cgc aaa cgt 1664 Gln Val Leu Leu His Lys Ile Pro Pro Arg Lys Arg Leu Arg Lys Arg 410 415 420 gac tca caa gtt aaa agt atg aaa cat aat tca cgt gta aaa att cat 1712 Asp Ser Gln Val Lys Ser Met Lys His Asn Ser Arg Val Lys Ile His 425 430 435 cag aag agc tgt gaa agg caa aag gcc aag gaa ggc aat ggt tgt agg 1760 Gln Lys Ser Cys Glu Arg Gln Lys Ala Lys Glu Gly Asn Gly Cys Arg 440 445 450 aaa acc ttc agt cgg agt act aaa cag att acg ttt ata aga att cac 1808 Lys Thr Phe Ser Arg Ser Thr Lys Gln Ile Thr Phe Ile Arg Ile His 455 460 465 aag ggg agc caa gtt tgc cga tgc agt gaa tgt ggt aaa ata ttc cgg 1856 Lys Gly Ser Gln Val Cys Arg Cys Ser Glu Cys Gly Lys Ile Phe Arg 470 475 480 485 aac cca aga tac ttt tct gtg cat aag aaa atc cat acc gga gag agg 1904 Asn Pro Arg Tyr Phe Ser Val His Lys Lys Ile His Thr Gly Glu Arg 490 495 500 ccc tat gtg tgt caa gac tgt ggg aaa gga ttt gtt cag agc tct tcc 1952 Pro Tyr Val Cys Gln Asp Cys Gly Lys Gly Phe Val Gln Ser Ser Ser 505 510 515 ctc aca cag cat cag aga gtt cat tct gga gag aga cca ttt gaa tgt 2000 Leu Thr Gln His Gln Arg Val His Ser Gly Glu Arg Pro Phe Glu Cys 520 525 530 cag gag tgt ggg agg acc ttc aat gat cgc tca gcc atc tcc cag cac 2048 Gln Glu Cys Gly Arg Thr Phe Asn Asp Arg Ser Ala Ile Ser Gln His 535 540 545 ctg agg act cac act ggc gct aag ccc tac aag tgt cag gac tgt gga 2096 Leu Arg Thr His Thr Gly Ala Lys Pro Tyr Lys Cys Gln Asp Cys Gly 550 555 560 565 aaa gcc ttc cgc cag agc tcc cac ctc atc aga cat cag agg act cac 2144 Lys Ala Phe Arg Gln Ser Ser His Leu Ile Arg His Gln Arg Thr His 570 575 580 acc ggg gag cgc cca tat gca tgc aac aaa tgt gga aag gcc ttc acc 2192 Thr Gly Glu Arg Pro Tyr Ala Cys Asn Lys Cys Gly Lys Ala Phe Thr 585 590 595 cag agc tca cac ctt att ggg cac cag aga acc cac aat agg aca aag 2240 Gln Ser Ser His Leu Ile Gly His Gln Arg Thr His Asn Arg Thr Lys 600 605 610 cga aag aag aaa cag cct acc tca tagctctcaa gccagttgaa gaaaccttgc 2294 Arg Lys Lys Lys Gln Pro Thr Ser 615 620 cttttcagct tgaccctgca atataacatg cacaggcctg cttgtgaatc aggactgaat 2354 gtgaaaggga agtattgagt gaggacattc ccaaaaccaa aggacaactg aggagactgc 2414 ccagcacata atgaataaat aagaaaatga gtgaggagtt attaacatca tttggaaaaa 2474 agatttccca ttcacttgat attgtttgtt cactcattta gtcattaaaa gtgagattaa 2534 taaaatctga aaatgttata taataacttt aaaaagccag gtaattaata atctgcactg 2594 atattacatc cacagtacca cagtatttat gtgtatgaat taaggattaa aagataatgt 2654 ggataaataa actattgatc tatgaaaaaa aaaaaaaaaa 2694 <210> 100 <211> 6193 <212> DNA <213> Homo sapiens <220> <223> clone name: hfb341 <220> <221> CDS <222> (466)..(3870) <400> 100 gaaaaaacag ctcacaactt ggagaacgta ctgatacatt tctgggaaag actgtcagag 60 atctgtgttg cgaaaatcag tgagccagaa gctgatgttg agtccgtttt gggtgtatct 120 aacctattac aggtgcttca gaagccgaag agctcattga agtcaagtaa aaaaaaaaat 180 ggtaaggtta gatttgctga tgagatactt gaaagcaata aagagaatga aaaatgtgta 240 tcttcagaag gagagaagat tgaaggctgg gaattaacaa ctgaaccttc tctcactcat 300 aattcttcag gccttttgtc tcctctaagg aaaaaacctt tggaagactt agtctgtaaa 360 ctcgcagata taagtattaa ttatgtcaat gaacgaaagt cagagcaaca tctaaggttt 420 ctttctactc tgcttgactc cttttcttca agccgagtat ttaaa atg cta ctt ggt 477 Met Leu Leu Gly 1 gat gaa aaa cag agt att gtc caa gcc aaa cct ctt gaa ata gcc aag 525 Asp Glu Lys Gln Ser Ile Val Gln Ala Lys Pro Leu Glu Ile Ala Lys 5 10 15 20 ctt gta caa aaa aat cct gcg gtg cag ttt tta tac cag aaa ctg ata 573 Leu Val Gln Lys Asn Pro Ala Val Gln Phe Leu Tyr Gln Lys Leu Ile 25 30 35 ggt tgg cta aat gaa gat caa agg aag gat ttt ggt ttc ctg gtg gac 621 Gly Trp Leu Asn Glu Asp Gln Arg Lys Asp Phe Gly Phe Leu Val Asp 40 45 50 att ttg tac agt gct ctc cgg tgc tgt gac aat gat atg gaa aga aaa 669 Ile Leu Tyr Ser Ala Leu Arg Cys Cys Asp Asn Asp Met Glu Arg Lys 55 60 65 aaa gtc ttg gat gat cta acc aag gtg gac ttg aaa tgg aat tct ctt 717 Lys Val Leu Asp Asp Leu Thr Lys Val Asp Leu Lys Trp Asn Ser Leu 70 75 80 ctt aag att att gaa aag gca tgt cct agt tca gat aaa cat gct tta 765 Leu Lys Ile Ile Glu Lys Ala Cys Pro Ser Ser Asp Lys His Ala Leu 85 90 95 100 gta act cct tgg ctc aaa ggc gat atc ctt ggt gag aaa ttg gtc aac 813 Val Thr Pro Trp Leu Lys Gly Asp Ile Leu Gly Glu Lys Leu Val Asn 105 110 115 ttg gca gat tgt ctt tgt aat gag gac ttg gaa tcc agg gta tct tca 861 Leu Ala Asp Cys Leu Cys Asn Glu Asp Leu Glu Ser Arg Val Ser Ser 120 125 130 gaa tct cac ttc tca gaa aga tgg act ctt cta agc ttg gta tta tcc 909 Glu Ser His Phe Ser Glu Arg Trp Thr Leu Leu Ser Leu Val Leu Ser 135 140 145 caa cat gtt aaa aat gat tac ttg att gga gac gta tat gtt gaa aga 957 Gln His Val Lys Asn Asp Tyr Leu Ile Gly Asp Val Tyr Val Glu Arg 150 155 160 atc att gtt aga ctt cat gaa act tta ttc aaa aca aag aaa tta tca 1005 Ile Ile Val Arg Leu His Glu Thr Leu Phe Lys Thr Lys Lys Leu Ser 165 170 175 180 gaa gct gaa agc agt gac tca tca gtg tct ttt atc tgt gat gtg gcc 1053 Glu Ala Glu Ser Ser Asp Ser Ser Val Ser Phe Ile Cys Asp Val Ala 185 190 195 tat aac tat ttc agc tca gcg aaa gga tgc ttg cta atg cca tca tct 1101 Tyr Asn Tyr Phe Ser Ser Ala Lys Gly Cys Leu Leu Met Pro Ser Ser 200 205 210 gaa gat tta tta tta act ctc ttt cag tta tgt gct cag agc aaa gaa 1149 Glu Asp Leu Leu Leu Thr Leu Phe Gln Leu Cys Ala Gln Ser Lys Glu 215 220 225 aaa aca cat ttg cca gat ttt ctt atc tgt aaa ctg aaa aat act tgg 1197 Lys Thr His Leu Pro Asp Phe Leu Ile Cys Lys Leu Lys Asn Thr Trp 230 235 240 ctc tct ggt gta aat tta ttg gtt cat caa act gac agt tca tat aaa 1245 Leu Ser Gly Val Asn Leu Leu Val His Gln Thr Asp Ser Ser Tyr Lys 245 250 255 260 gag agt acc ttc cta cat ttg tct gct ctg tgg ctg aag aac caa gtt 1293 Glu Ser Thr Phe Leu His Leu Ser Ala Leu Trp Leu Lys Asn Gln Val 265 270 275 cag gct tca tct ttg gat atc aac agt ctc caa gtc ctc ttg tct gct 1341 Gln Ala Ser Ser Leu Asp Ile Asn Ser Leu Gln Val Leu Leu Ser Ala 280 285 290 gtt gat gat ttg cta aat aca ctt cta gag agt gaa gat tct tat ctt 1389 Val Asp Asp Leu Leu Asn Thr Leu Leu Glu Ser Glu Asp Ser Tyr Leu 295 300 305 atg gga gtt tat att gga agt gta atg ccg aac gac agt gaa tgg gaa 1437 Met Gly Val Tyr Ile Gly Ser Val Met Pro Asn Asp Ser Glu Trp Glu 310 315 320 aag atg agg cag tct ctt cct atg cag tgg tta cat aga cct ctt tta 1485 Lys Met Arg Gln Ser Leu Pro Met Gln Trp Leu His Arg Pro Leu Leu 325 330 335 340 gag gga aga ttg agt ttg aat tat gaa tgt ttc aaa aca gat ttt aag 1533 Glu Gly Arg Leu Ser Leu Asn Tyr Glu Cys Phe Lys Thr Asp Phe Lys 345 350 355 gaa cag gac ata aag aca ctt ccc agc cat ttg tgt act tca gca tta 1581 Glu Gln Asp Ile Lys Thr Leu Pro Ser His Leu Cys Thr Ser Ala Leu 360 365 370 ttg agc aaa atg gtc tta att gca ctg aga aag gaa aca gtc tta gaa 1629 Leu Ser Lys Met Val Leu Ile Ala Leu Arg Lys Glu Thr Val Leu Glu 375 380 385 aat aat gag ctt gag aaa ata att gca gaa ctg ctt tat tca ctg cag 1677 Asn Asn Glu Leu Glu Lys Ile Ile Ala Glu Leu Leu Tyr Ser Leu Gln 390 395 400 tgg tgt gaa gaa tta gat aac cca cct att ttt cta att gga ttt tgt 1725 Trp Cys Glu Glu Leu Asp Asn Pro Pro Ile Phe Leu Ile Gly Phe Cys 405 410 415 420 gaa ata ctt caa aaa atg aat att acg tat gat aac tta cgt gta ctt 1773 Glu Ile Leu Gln Lys Met Asn Ile Thr Tyr Asp Asn Leu Arg Val Leu 425 430 435 ggt aat acg tcg ggc ctt ttg cag ctg tta ttt aac agg tcc aga gaa 1821 Gly Asn Thr Ser Gly Leu Leu Gln Leu Leu Phe Asn Arg Ser Arg Glu 440 445 450 cat ggc aca ctg tgg tct ctt att att gct aag ttg atc ctt tcc cga 1869 His Gly Thr Leu Trp Ser Leu Ile Ile Ala Lys Leu Ile Leu Ser Arg 455 460 465 agc att tca tct gat gaa gta aaa cca cat tat aag aga aaa gaa agt 1917 Ser Ile Ser Ser Asp Glu Val Lys Pro His Tyr Lys Arg Lys Glu Ser 470 475 480 ttt ttt cca cta act gaa ggc aat ttg cat acc att caa agt cta tgt 1965 Phe Phe Pro Leu Thr Glu Gly Asn Leu His Thr Ile Gln Ser Leu Cys 485 490 495 500 cca ttt ttg tca aaa gaa gaa aag aaa gaa ttt agt gct caa tgt ata 2013 Pro Phe Leu Ser Lys Glu Glu Lys Lys Glu Phe Ser Ala Gln Cys Ile 505 510 515 cct gct ctt ttg ggc tgg act aag aaa gat ctt tgc agc act aat gga 2061 Pro Ala Leu Leu Gly Trp Thr Lys Lys Asp Leu Cys Ser Thr Asn Gly 520 525 530 ggt ttt gga cat ctt gcc att ttc aat tct tgt ctg caa acc aaa agt 2109 Gly Phe Gly His Leu Ala Ile Phe Asn Ser Cys Leu Gln Thr Lys Ser 535 540 545 ata gat gat gga gag cta tta cat gga ata tta aaa atc ata ata tcc 2157 Ile Asp Asp Gly Glu Leu Leu His Gly Ile Leu Lys Ile Ile Ile Ser 550 555 560 tgg aag aaa gag cat gaa gat att ttt ctt ttc agt tgt aat cta tca 2205 Trp Lys Lys Glu His Glu Asp Ile Phe Leu Phe Ser Cys Asn Leu Ser 565 570 575 580 gaa gca agt cca gag gta ctg ggt gta aat ata gaa ata atc cgg ttt 2253 Glu Ala Ser Pro Glu Val Leu Gly Val Asn Ile Glu Ile Ile Arg Phe 585 590 595 ctt tcc cta ttt ctg aaa tac tgc tca tcc cct ttg gca gag agt gag 2301 Leu Ser Leu Phe Leu Lys Tyr Cys Ser Ser Pro Leu Ala Glu Ser Glu 600 605 610 tgg gac ttc atc atg tgc tcc atg ttg gct tgg ctg gag aca aca agt 2349 Trp Asp Phe Ile Met Cys Ser Met Leu Ala Trp Leu Glu Thr Thr Ser 615 620 625 gag aat cag gca ttg tat tct att cca ctt gtg caa ctg ttt gcc tgt 2397 Glu Asn Gln Ala Leu Tyr Ser Ile Pro Leu Val Gln Leu Phe Ala Cys 630 635 640 gtc agc tgt gat ttg gcc tgt gac ctc agt gct ttc ttt gat tcc aca 2445 Val Ser Cys Asp Leu Ala Cys Asp Leu Ser Ala Phe Phe Asp Ser Thr 645 650 655 660 act ctg gat acc att ggc aat ctt cct gta aat cta atc agt gaa tgg 2493 Thr Leu Asp Thr Ile Gly Asn Leu Pro Val Asn Leu Ile Ser Glu Trp 665 670 675 aaa gaa ttt ttt tcc caa ggc atc cac agt ttg ctt tta cct att ttg 2541 Lys Glu Phe Phe Ser Gln Gly Ile His Ser Leu Leu Leu Pro Ile Leu 680 685 690 gtg act gtt aca gga gaa aac aaa gat gtg tct gaa aca tcc ttt cag 2589 Val Thr Val Thr Gly Glu Asn Lys Asp Val Ser Glu Thr Ser Phe Gln 695 700 705 aat gca atg ctg aaa ccc atg tgt gaa aca tta acg tat atc tca aag 2637 Asn Ala Met Leu Lys Pro Met Cys Glu Thr Leu Thr Tyr Ile Ser Lys 710 715 720 gaa cag cta ttg agt cac aaa ctt cct gca aga tta gtt gct gac caa 2685 Glu Gln Leu Leu Ser His Lys Leu Pro Ala Arg Leu Val Ala Asp Gln 725 730 735 740 aaa aca aac tta cca gaa tat ctc cag act ttg tta aat aca ttg gcc 2733 Lys Thr Asn Leu Pro Glu Tyr Leu Gln Thr Leu Leu Asn Thr Leu Ala 745 750 755 cca tta ctc ctc ttc aga gct agg cct gtg caa att gct gtt tat cat 2781 Pro Leu Leu Leu Phe Arg Ala Arg Pro Val Gln Ile Ala Val Tyr His 760 765 770 atg cta tac aaa ttg atg cct gaa tta cca cag tat gat cag gat aat 2829 Met Leu Tyr Lys Leu Met Pro Glu Leu Pro Gln Tyr Asp Gln Asp Asn 775 780 785 cta aag tca tac gga gat gaa gaa gaa gag cca gcc ttg tca cca cca 2877 Leu Lys Ser Tyr Gly Asp Glu Glu Glu Glu Pro Ala Leu Ser Pro Pro 790 795 800 gca gca ctg atg tct ctt ctt agc att caa gag gac tta cta gaa aat 2925 Ala Ala Leu Met Ser Leu Leu Ser Ile Gln Glu Asp Leu Leu Glu Asn 805 810 815 820 gtt ttg ggg tgt att cct gtt gga cag ata gtt act att aaa cca ctg 2973 Val Leu Gly Cys Ile Pro Val Gly Gln Ile Val Thr Ile Lys Pro Leu 825 830 835 agt gaa gac ttc tgt tat gtt ctg gga tac ctt ctc act tgg aaa tta 3021 Ser Glu Asp Phe Cys Tyr Val Leu Gly Tyr Leu Leu Thr Trp Lys Leu 840 845 850 ata cta act ttc ttc aaa gct gca tca tca cag ctt cgg gct ttg tat 3069 Ile Leu Thr Phe Phe Lys Ala Ala Ser Ser Gln Leu Arg Ala Leu Tyr 855 860 865 tcc atg tat ctt cgg aaa aca aag agt ttg aat aaa ttg ctc tat cac 3117 Ser Met Tyr Leu Arg Lys Thr Lys Ser Leu Asn Lys Leu Leu Tyr His 870 875 880 ctg ttc agg ctt atg cca gaa aat cca acc tat gca gag aca gca gtt 3165 Leu Phe Arg Leu Met Pro Glu Asn Pro Thr Tyr Ala Glu Thr Ala Val 885 890 895 900 gag gtc cca aat aag gac cct aaa aca ttc ttt act gag gag ctc cag 3213 Glu Val Pro Asn Lys Asp Pro Lys Thr Phe Phe Thr Glu Glu Leu Gln 905 910 915 ctg agt att aga gaa aca aca atg ctt cca tac cac att cca cac ttg 3261 Leu Ser Ile Arg Glu Thr Thr Met Leu Pro Tyr His Ile Pro His Leu 920 925 930 gct tgt tca gtc tat cat atg aca tta aaa gac ttg cct gcc atg gtt 3309 Ala Cys Ser Val Tyr His Met Thr Leu Lys Asp Leu Pro Ala Met Val 935 940 945 agg ttg tgg tgg aat agc agt gag aag cgt gtt ttc aat att gtg gat 3357 Arg Leu Trp Trp Asn Ser Ser Glu Lys Arg Val Phe Asn Ile Val Asp 950 955 960 aga ttt aca agc aag tat gtc agc agt gtt ctt tct ttt caa gaa ata 3405 Arg Phe Thr Ser Lys Tyr Val Ser Ser Val Leu Ser Phe Gln Glu Ile 965 970 975 980 tct tct gta caa aca agt aca caa cta ttt aat ggc atg acg gtt aaa 3453 Ser Ser Val Gln Thr Ser Thr Gln Leu Phe Asn Gly Met Thr Val Lys 985 990 995 gct cga gct act act cga gag gta atg gct act tat act att gag gac 3501 Ala Arg Ala Thr Thr Arg Glu Val Met Ala Thr Tyr Thr Ile Glu Asp 1000 1005 1010 ata gtt att gaa ctt ata ata caa ctg cct tca aat tat cca ctg ggt 3549 Ile Val Ile Glu Leu Ile Ile Gln Leu Pro Ser Asn Tyr Pro Leu Gly 1015 1020 1025 tca ata ata gta gaa agt ggg aaa aga gta gga gta gct gtt cag cag 3597 Ser Ile Ile Val Glu Ser Gly Lys Arg Val Gly Val Ala Val Gln Gln 1030 1035 1040 tgg cgg aac tgg atg ctg cag tta agc act tac ctc acc cat cag aat 3645 Trp Arg Asn Trp Met Leu Gln Leu Ser Thr Tyr Leu Thr His Gln Asn 1045 1050 1055 1060 gga agt att atg gaa ggc tta gct tta tgg aaa aat aac gta gac aaa 3693 Gly Ser Ile Met Glu Gly Leu Ala Leu Trp Lys Asn Asn Val Asp Lys 1065 1070 1075 cgt ttt gag ggt gtt gaa gat tgc atg atc tgt ttc tca gtc att cac 3741 Arg Phe Glu Gly Val Glu Asp Cys Met Ile Cys Phe Ser Val Ile His 1080 1085 1090 ggt ttc aac tat tcc ctt ccc aaa aaa gcc tgt aga aca tgc aag aaa 3789 Gly Phe Asn Tyr Ser Leu Pro Lys Lys Ala Cys Arg Thr Cys Lys Lys 1095 1100 1105 aaa ttc cat tca gcc tgc ttg tac aaa tgg ttt aca tct agc aac aaa 3837 Lys Phe His Ser Ala Cys Leu Tyr Lys Trp Phe Thr Ser Ser Asn Lys 1110 1115 1120 tcc act tgt cca ctg tgt cgt gag acg ttt ttc tgagattttt ttcactggaa 3890 Ser Thr Cys Pro Leu Cys Arg Glu Thr Phe Phe 1125 1130 1135 gggatccctg aagtacatca aacaaaggca ttggatttgg atccgtctga aagtgtggat 3950 gtggggaagc cagtgagcat tacttttaaa taggaccttc tctggaaaat tattttggtt 4010 aatgtaataa tcctaaaatc aggtttactt aactttagat tgctttgtaa atgtttggaa 4070 actttctttt acaaaagaat attacatatt tgagagaaaa tatttctatt aatggtttaa 4130 tttctttgta tatttaaaaa taaatatgaa aaacccctaa attacagaat tggaatttgt 4190 gaaaagattg cacaactata ttattgataa acccattccg tttgttgatc tgtgttttag 4250 aatagtccaa gtgaagcaaa acgaactgag agagctgatt tacatctata gatattttaa 4310 gccttattta ttagttatga caataaatta gtattctgat atttgtagat tattttaatg 4370 ataaaaatga cactaagcta ttaatacggc ttgtcttaag atatctggag gcatgctctg 4430 aaatcctata taaaagagtt taaactgaac tttaaggtgc ctccatttat caagggttat 4490 gaaactcaga aaattaaatt cttgatgctg acctttttgc ttgtgggaga attatttcta 4550 tatattagtg aaatttgaat tgtaaacttg tcagtgaatt tatcactctg agcgagtctc 4610 tagagggtga gaatttgaga gaaatagtaa tttgattatt tcattcatga agcggagtgg 4670 tagttatcct tctattgaga gggaagaaat aaatgagtat tactgaaact taaagatcag 4730 tggaaaaaaa aaacatttaa aaatgttgta ggagactttc aattttggaa gcaaactgag 4790 ctattcttac cagccaaagt tatactaaat aagagacttg ggggagcaaa tgtttttcag 4850 ccttcagaaa tgaaagttaa ggattttagc actaggtaaa attcagtata ataggctgaa 4910 gtggagtaag tgaaaacctg ccttttgcca ctcttaaaaa ttgtgcccaa aatataaaag 4970 tgtggaactt tagaacttgg aataatttta ttgcagtctt ccattacatg gaaatagcat 5030 atctaatatc taggttactt gagagaccag ctaatcatct ctgttgcaca tgtttaattg 5090 gcaaaaagca attcatgata aataaaatta ctgctttcca tctactgggt aaaatgacta 5150 ttgaaatagt atgaatgtgg tcagaggatt atagttgaga gtgaagtact atgtgtgagt 5210 tatagatctc tcgaattata tttatagatg cagtgtcctg cccagttttg tttgcctcct 5270 acattttact gtaaaatatt tattgtcttc tagccttgag cctctgaggg tcagtaagtg 5330 aagtacagat agcaaaattt tactacctcg ttaacccttt tcttaaaata ttctctatct 5390 ttctatgtct ctttctgaca tagtaatccc aaaggattgt gttactcccc gtgaaagtta 5450 ttacttttcc tttaaaaatg gttttataat aagactgttt aaaacctttc cagtattggt 5510 acatcttggc ttctggccca aaccccaagc agaaaagaaa atggaataat ggagcattgt 5570 ttttccacat tagtattagg gcatcagatt cttggtgaaa cactataatt aagtagttat 5630 aattaaataa ctgttcttca tacttaggac tcttaataca tttctttaag acttgtaagt 5690 ataattgtaa agcttgttaa ctgtttatat actaaagaaa aagctttaca agataagact 5750 gaaattcaga ataccacatc tgtacagcgt tgacaactct aactaaaagg attacagccc 5810 ttaaaatttt agcttaagga cattttaaaa ttgcatgaga ttagcaaaat tattaattta 5870 aaaccccaaa gtaaattgca aattttaaaa tttatttttg actcttttat cacatgctcc 5930 aatgtatgta cataagtgtc tgtggcttaa ataatatgtt gttatttgat acatttttgt 5990 ggtgcaagta attaatgtta ttatttgatt gttataccat atgtgcccat taaaattctt 6050 tttaattaaa aatatttcaa atatgcagaa aaataataat ataaatgccc ttgtaccctt 6110 cacatactat tttgccatat ttgcttctga tttttaaata aataaaatgt tactcttttg 6170 taaaaaaaaa aaaaaaaaaa aaa 6193 <210> 101 <211> 474 <212> PRT <213> Homo sapiens <220> <223> Designation: HFB030 <400> 101 Met Ser Ser Glu Asp Arg Glu Ala Gln Glu Asp Glu Leu Leu Ala Leu 1 5 10 15 Ala Ser Ile Tyr Asp Gly Asp Glu Phe Arg Lys Ala Glu Ser Val Gln 20 25 30 Gly Gly Glu Thr Arg Ile Tyr Leu Asp Leu Pro Gln Asn Phe Lys Ile 35 40 45 Phe Val Ser Gly Asn Ser Asn Glu Cys Leu Gln Asn Ser Gly Phe Glu 50 55 60 Tyr Thr Ile Cys Phe Leu Pro Pro Leu Val Leu Asn Phe Glu Leu Pro 65 70 75 80 Pro Asp Tyr Pro Ser Ser Ser Pro Pro Ser Phe Thr Leu Ser Gly Lys 85 90 95 Trp Leu Ser Pro Thr Gln Leu Ser Ala Leu Cys Lys His Leu Asp Asn 100 105 110 Leu Trp Glu Glu His Arg Gly Ser Val Val Leu Phe Ala Trp Met Gln 115 120 125 Phe Leu Lys Glu Glu Thr Leu Ala Tyr Leu Asn Ile Val Ser Pro Phe 130 135 140 Glu Leu Lys Ile Gly Ser Gln Lys Lys Val Gln Arg Arg Thr Ala Gln 145 150 155 160 Ala Ser Pro Asn Thr Glu Leu Asp Phe Gly Gly Ala Ala Gly Ser Asp 165 170 175 Val Asp Gln Glu Glu Ile Val Asp Glu Arg Ala Val Gln Asp Val Glu 180 185 190 Ser Leu Ser Asn Leu Ile Gln Glu Ile Leu Asp Phe Asp Gln Ala Gln 195 200 205 Gln Ile Lys Cys Phe Asn Ser Lys Leu Phe Leu Cys Ser Ile Cys Phe 210 215 220 Cys Glu Lys Leu Gly Ser Glu Cys Met Tyr Phe Leu Glu Cys Arg His 225 230 235 240 Val Tyr Cys Lys Ala Cys Leu Lys Asp Tyr Phe Glu Ile Gln Ile Arg 245 250 255 Asp Gly Gln Val Gln Cys Leu Asn Cys Pro Glu Pro Lys Cys Pro Ser 260 265 270 Val Ala Thr Pro Gly Gln Val Lys Glu Leu Val Glu Ala Glu Leu Phe 275 280 285 Ala Arg Tyr Asp Arg Leu Leu Leu Gln Ser Ser Leu Asp Leu Met Ala 290 295 300 Asp Val Val Tyr Cys Pro Arg Pro Cys Cys Gln Leu Pro Val Met Gln 305 310 315 320 Glu Pro Gly Cys Thr Met Gly Ile Cys Ser Ser Cys Asn Phe Ala Phe 325 330 335 Cys Thr Leu Cys Arg Leu Thr Tyr His Gly Val Ser Pro Cys Lys Val 340 345 350 Thr Ala Glu Lys Leu Met Asp Leu Arg Asn Glu Tyr Leu Gln Ala Asp 355 360 365 Glu Ala Asn Lys Arg Leu Leu Asp Gln Arg Tyr Gly Lys Arg Val Ile 370 375 380 Gln Lys Ala Leu Glu Glu Met Glu Ser Lys Glu Trp Leu Glu Lys Asn 385 390 395 400 Ser Lys Ser Cys Pro Cys Cys Gly Thr Pro Ile Glu Lys Leu Asp Gly 405 410 415 Cys Asn Lys Met Thr Cys Thr Gly Cys Met Gln Tyr Phe Cys Trp Ile 420 425 430 Cys Met Gly Ser Leu Ser Arg Ala Asn Pro Tyr Lys His Phe Asn Asp 435 440 445 Pro Gly Ser Pro Cys Phe Asn Arg Leu Phe Tyr Ala Val Asp Val Asp 450 455 460 Asp Asp Ile Trp Glu Asp Glu Val Glu Asp 465 470 <210> 102 <211> 895 <212> PRT <213> Homo sapiens <220> <223> Designation: HFB044-1 <400> 102 Met Lys Ile Gly Ser Gly Phe Leu Ser Gly Gly Gly Gly Thr Gly Ser 1 5 10 15 Ser Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly 20 25 30 Gly Gly Ser Ser Gly Arg Arg Ala Glu Met Glu Pro Thr Phe Pro Gln 35 40 45 Gly Met Val Met Phe Asn His Arg Leu Pro Pro Val Thr Ser Phe Thr 50 55 60 Arg Pro Ala Gly Ser Ala Ala Pro Pro Pro Gln Cys Val Leu Ser Ser 65 70 75 80 Ser Thr Ser Ala Ala Pro Ala Ala Glu Pro Pro Pro Pro Pro Ala Pro 85 90 95 Asp Met Thr Phe Lys Lys Glu Pro Ala Ala Ser Ala Ala Ala Phe Pro 100 105 110 Ser Gln Arg Thr Ser Trp Gly Phe Leu Gln Ser Leu Val Thr Ile Lys 115 120 125 Gln Glu Lys Pro Ala Asp Pro Glu Glu Gln Gln Ser His His His His 130 135 140 His His His His Tyr Gly Gly Leu Phe Ala Gly Ala Glu Glu Arg Ser 145 150 155 160 Pro Gly Leu Gly Gly Gly Glu Gly Gly Ser His Gly Val Ile Gln Asp 165 170 175 Leu Ser Ile Leu His Gln His Val Gln Gln Gln Pro Ala Gln His His 180 185 190 Arg Asp Val Leu Leu Ser Ser Ser Ser Arg Thr Asp Asp His His Gly 195 200 205 Thr Glu Glu Pro Lys Gln Asp Thr Asn Val Lys Lys Ala Lys Arg Pro 210 215 220 Lys Pro Glu Ser Gln Gly Ile Lys Ala Lys Arg Lys Pro Ser Ala Ser 225 230 235 240 Ser Lys Pro Ser Leu Val Gly Asp Gly Glu Gly Ala Ile Leu Ser Pro 245 250 255 Ser Gln Lys Pro His Ile Cys Asp His Cys Ser Ala Ala Phe Arg Ser 260 265 270 Ser Tyr His Leu Arg Arg His Val Leu Ile His Thr Gly Glu Arg Pro 275 280 285 Phe Gln Cys Ser Gln Cys Ser Met Gly Phe Ile Gln Lys Tyr Leu Leu 290 295 300 Gln Arg His Glu Lys Ile His Ser Arg Glu Lys Pro Phe Gly Cys Asp 305 310 315 320 Gln Cys Ser Met Lys Phe Ile Gln Lys Tyr His Met Glu Arg His Lys 325 330 335 Arg Thr His Ser Gly Glu Lys Pro Tyr Lys Cys Asp Thr Cys Gln Gln 340 345 350 Tyr Phe Ser Arg Thr Asp Arg Leu Leu Lys His Arg Arg Thr Cys Gly 355 360 365 Glu Val Ile Val Lys Gly Ala Thr Ser Ala Glu Pro Gly Ser Ser Asn 370 375 380 His Thr Asn Met Gly Asn Leu Ala Val Leu Ser Gln Gly Asn Thr Ser 385 390 395 400 Ser Ser Arg Arg Lys Thr Lys Ser Lys Ser Ile Ala Ile Glu Asn Lys 405 410 415 Glu Gln Lys Thr Gly Lys Thr Asn Glu Ser Gln Ile Ser Asn Asn Ile 420 425 430 Asn Met Gln Ser Tyr Ser Val Glu Met Pro Thr Val Ser Ser Ser Gly 435 440 445 Gly Ile Ile Gly Thr Gly Ile Asp Glu Leu Gln Lys Arg Val Pro Lys 450 455 460 Leu Ile Phe Lys Lys Gly Ser Arg Lys Asn Thr Asp Lys Asn Tyr Leu 465 470 475 480 Asn Phe Val Ser Pro Leu Pro Asp Ile Val Gly Gln Lys Ser Leu Ser 485 490 495 Gly Lys Pro Ser Gly Ser Leu Gly Ile Val Ser Asn Asn Ser Val Glu 500 505 510 Thr Ile Gly Leu Leu Gln Ser Thr Ser Gly Lys Gln Gly Gln Ile Ser 515 520 525 Ser Asn Tyr Asp Asp Ala Met Gln Phe Ser Lys Lys Arg Arg Tyr Leu 530 535 540 Pro Thr Ala Ser Ser Asn Ser Ala Phe Ser Ile Asn Val Gly His Met 545 550 555 560 Val Ser Gln Gln Ser Val Ile Gln Ser Ala Gly Val Ser Val Leu Asp 565 570 575 Asn Glu Ala Pro Leu Ser Leu Ile Asp Ser Ser Ala Leu Asn Ala Glu 580 585 590 Ile Lys Ser Cys His Asp Lys Ser Gly Ile Pro Asp Glu Val Leu Gln 595 600 605 Ser Ile Leu Asp Gln Tyr Ser Asn Lys Ser Glu Ser Gln Lys Glu Asp 610 615 620 Pro Phe Asn Ile Ala Glu Pro Arg Val Asp Leu His Thr Ser Gly Glu 625 630 635 640 His Ser Glu Leu Val Gln Glu Glu Asn Leu Ser Pro Gly Thr Gln Thr 645 650 655 Pro Ser Asn Asp Lys Ala Ser Met Leu Gln Glu Tyr Ser Lys Tyr Leu 660 665 670 Gln Gln Ala Phe Glu Lys Ser Thr Asn Ala Ser Phe Thr Leu Gly His 675 680 685 Gly Phe Gln Phe Val Ser Leu Ser Ser Pro Leu His Asn His Thr Leu 690 695 700 Phe Pro Glu Lys Gln Ile Tyr Thr Thr Ser Pro Leu Glu Cys Gly Phe 705 710 715 720 Gly Gln Ser Val Thr Ser Val Leu Pro Ser Ser Leu Pro Lys Pro Pro 725 730 735 Phe Gly Met Leu Phe Gly Ser Gln Pro Gly Leu Tyr Leu Ser Ala Leu 740 745 750 Asp Ala Thr His Gln Gln Leu Thr Pro Ser Gln Glu Leu Asp Asp Leu 755 760 765 Ile Asp Ser Gln Lys Asn Leu Glu Thr Ser Ser Ala Phe Gln Ser Ser 770 775 780 Ser Gln Lys Leu Thr Ser Gln Lys Glu Gln Lys Asn Leu Glu Ser Ser 785 790 795 800 Thr Gly Phe Gln Ile Pro Ser Gln Glu Leu Ala Ser Gln Ile Asp Pro 805 810 815 Gln Lys Asp Ile Glu Pro Arg Thr Thr Tyr Gln Ile Glu Asn Phe Ala 820 825 830 Gln Ala Phe Gly Ser Gln Phe Lys Ser Gly Ser Arg Val Pro Met Thr 835 840 845 Phe Ile Thr Asn Ser Asn Gly Glu Val Asp His Arg Val Arg Thr Ser 850 855 860 Val Ser Asp Phe Ser Gly Tyr Thr Asn Met Met Ser Asp Val Ser Glu 865 870 875 880 Pro Cys Ser Thr Arg Val Lys Thr Pro Thr Ser Gln Ser Tyr Arg 885 890 895 <210> 103 <211> 895 <212> PRT <213> Homo sapiens <220> <223> Designation: HFB044-2 <400> 103 Met Lys Ile Gly Ser Gly Phe Leu Ser Gly Gly Gly Gly Thr Gly Ser 1 5 10 15 Ser Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly 20 25 30 Gly Gly Ser Ser Gly Arg Arg Ala Glu Met Glu Pro Thr Phe Pro Gln 35 40 45 Gly Met Val Met Phe Asn His Arg Leu Pro Pro Val Thr Ser Phe Thr 50 55 60 Arg Pro Ala Gly Ser Ala Ala Pro Pro Pro Gln Cys Val Leu Ser Ser 65 70 75 80 Ser Thr Ser Ala Ala Pro Ala Ala Glu Pro Pro Pro Pro Pro Ala Pro 85 90 95 Asp Met Thr Phe Lys Lys Glu Pro Ala Ala Ser Ala Ala Ala Phe Pro 100 105 110 Ser Gln Arg Thr Ser Trp Gly Phe Leu Gln Ser Leu Gly Ser Ile Lys 115 120 125 Gln Glu Lys Pro Ala Asp Pro Glu Glu Gln Gln Ser His His His His 130 135 140 His His His His Tyr Gly Gly Leu Phe Ala Gly Ala Glu Glu Arg Ser 145 150 155 160 Pro Gly Leu Gly Gly Gly Glu Gly Gly Ser His Gly Val Ile Gln Asp 165 170 175 Leu Ser Ile Leu His Gln His Val Gln Gln Gln Pro Ala Gln His His 180 185 190 Arg Asp Val Leu Leu Ser Ser Ser Ser Arg Thr Asp Asp His His Gly 195 200 205 Thr Glu Glu Pro Lys Gln Asp Thr Asn Val Lys Lys Ala Lys Arg Pro 210 215 220 Lys Pro Glu Ser Gln Gly Ile Lys Ala Lys Arg Lys Pro Ser Ala Ser 225 230 235 240 Ser Lys Pro Ser Leu Val Gly Asp Gly Glu Gly Ala Ile Leu Ser Pro 245 250 255 Ser Gln Lys Pro His Ile Cys Asp His Cys Ser Ala Ala Phe Arg Ser 260 265 270 Ser Tyr His Leu Arg Arg His Val Leu Ile His Thr Gly Glu Arg Pro 275 280 285 Phe Gln Cys Ser Gln Cys Ser Met Gly Phe Ile Gln Lys Tyr Leu Leu 290 295 300 Gln Arg His Glu Lys Ile His Ser Arg Glu Lys Pro Phe Gly Cys Asp 305 310 315 320 Gln Cys Ser Met Lys Phe Ile Gln Lys Tyr His Met Glu Arg His Lys 325 330 335 Arg Thr His Ser Gly Glu Lys Pro Tyr Lys Cys Asp Thr Cys Gln Gln 340 345 350 Tyr Phe Ser Arg Thr Asp Arg Leu Leu Lys His Arg Arg Thr Cys Gly 355 360 365 Glu Val Ile Val Lys Gly Ala Thr Ser Ala Glu Pro Gly Ser Ser Asn 370 375 380 His Thr Asn Met Gly Asn Leu Ala Val Leu Ser Gln Gly Asn Thr Ser 385 390 395 400 Ser Ser Arg Arg Lys Thr Lys Ser Lys Ser Ile Ala Ile Glu Asn Lys 405 410 415 Glu Gln Lys Thr Gly Lys Thr Asn Glu Ser Gln Ile Ser Asn Asn Ile 420 425 430 Asn Met Gln Ser Tyr Ser Val Glu Met Pro Thr Val Ser Ser Ser Gly 435 440 445 Gly Ile Ile Gly Thr Gly Ile Asp Glu Leu Gln Lys Arg Val Pro Lys 450 455 460 Leu Ile Phe Lys Lys Gly Ser Arg Lys Asn Thr Asp Lys Asn Tyr Leu 465 470 475 480 Asn Phe Val Ser Pro Leu Pro Asp Ile Val Gly Gln Lys Ser Leu Ser 485 490 495 Gly Lys Pro Ser Gly Ser Leu Gly Ile Val Ser Asn Asn Ser Val Glu 500 505 510 Thr Ile Gly Leu Leu Gln Ser Thr Ser Gly Lys Gln Gly Gln Ile Ser 515 520 525 Ser Asn Tyr Asp Asp Ala Met Gln Phe Ser Lys Lys Arg Arg Tyr Leu 530 535 540 Pro Thr Ala Ser Ser Asn Ser Ala Phe Ser Ile Asn Val Gly His Met 545 550 555 560 Val Ser Gln Gln Ser Val Ile Gln Ser Ala Gly Val Ser Val Leu Asp 565 570 575 Asn Glu Ala Pro Leu Ser Leu Ile Asp Ser Ser Ala Leu Asn Ala Glu 580 585 590 Ile Lys Ser Cys His Asp Lys Ser Gly Ile Pro Asp Glu Val Leu Gln 595 600 605 Ser Ile Leu Asp Gln Tyr Ser Asn Lys Ser Glu Ser Gln Lys Glu Asp 610 615 620 Pro Phe Asn Ile Ala Glu Pro Arg Val Asp Leu His Thr Ser Gly Glu 625 630 635 640 His Ser Glu Leu Val Gln Glu Glu Asn Leu Ser Pro Gly Thr Gln Thr 645 650 655 Pro Ser Asn Asp Lys Ala Ser Met Leu Gln Glu Tyr Ser Lys Tyr Leu 660 665 670 Gln Gln Ala Phe Glu Lys Ser Thr Asn Ala Ser Phe Thr Leu Gly His 675 680 685 Gly Phe Gln Phe Val Ser Leu Ser Ser Pro Leu His Asn His Thr Leu 690 695 700 Phe Pro Glu Lys Gln Ile Tyr Thr Thr Ser Pro Leu Glu Cys Gly Phe 705 710 715 720 Gly Gln Ser Val Thr Ser Val Leu Pro Ser Ser Leu Pro Lys Pro Pro 725 730 735 Phe Gly Met Leu Phe Gly Ser Gln Pro Gly Leu Tyr Leu Ser Ala Leu 740 745 750 Asp Ala Thr His Gln Gln Leu Thr Pro Ser Gln Glu Leu Asp Asp Leu 755 760 765 Ile Asp Ser Gln Lys Asn Leu Glu Thr Ser Ser Ala Phe Gln Ser Ser 770 775 780 Ser Gln Lys Leu Thr Ser Gln Lys Glu Gln Lys Asn Leu Glu Ser Ser 785 790 795 800 Thr Gly Phe Gln Ile Pro Ser Gln Glu Leu Ala Ser Gln Ile Asp Pro 805 810 815 Gln Lys Asp Ile Glu Pro Arg Thr Thr Tyr Gln Ile Glu Asn Phe Ala 820 825 830 Gln Ala Phe Gly Ser Gln Phe Lys Ser Gly Ser Arg Val Pro Met Thr 835 840 845 Phe Ile Thr Asn Ser Asn Gly Glu Val Asp His Arg Val Arg Thr Ser 850 855 860 Val Ser Asp Phe Ser Gly Tyr Thr Asn Met Met Ser Asp Val Ser Glu 865 870 875 880 Pro Cys Ser Thr Arg Val Lys Thr Pro Thr Ser Gln Ser Tyr Arg 885 890 895 <210> 104 <211> 367 <212> PRT <213> Homo sapiens <220> <223> Designation: HFB066 <400> 104 Lys Asn Tyr Lys Asn Ala Ser Gly Val Val Asn Ser Ser Pro Arg Ser 1 5 10 15 His Ser Ala Thr Asn Gly Ser Ile Pro Ser Ser Ser Ser Lys Asn Glu 20 25 30 Lys Lys Gln Lys Cys Thr Ser Lys Ser Pro Ser Thr His Lys Asp Leu 35 40 45 Met Glu Asn Cys Ile Pro Asn Asn Gln Leu Ser Lys Pro Asp Ala Leu 50 55 60 Val Arg Leu Glu Gln Asp Ile Lys Lys Leu Lys Ala Asp Leu Gln Ala 65 70 75 80 Ser Arg Gln Val Glu Gln Glu Leu Arg Ser Gln Ile Ser Ser Leu Ser 85 90 95 Ser Thr Glu Arg Gly Ile Arg Ser Glu Met Gly Gln Leu Arg Gln Glu 100 105 110 Asn Glu Leu Leu Gln Asn Lys Leu His Asn Ala Val Gln Met Lys Gln 115 120 125 Lys Asp Lys Gln Asn Ile Ser Gln Leu Glu Lys Lys Leu Lys Ala Glu 130 135 140 Gln Glu Ala Arg Ser Phe Val Glu Lys Gln Leu Met Glu Glu Lys Lys 145 150 155 160 Arg Lys Lys Leu Glu Glu Ala Thr Ala Ala Arg Ala Val Ala Phe Ala 165 170 175 Ala Ala Ser Arg Gly Glu Cys Thr Glu Thr Leu Arg Asn Arg Ile Arg 180 185 190 Glu Leu Glu Ala Glu Gly Lys Lys Leu Thr Met Asp Met Lys Val Lys 195 200 205 Glu Asp Gln Ile Arg Glu Leu Glu Leu Lys Val Gln Glu Leu Arg Lys 210 215 220 Tyr Lys Glu Asn Glu Lys Asp Thr Glu Val Leu Met Ser Ala Leu Ser 225 230 235 240 Ala Met Gln Asp Lys Thr Gln His Leu Glu Asn Ser Leu Ser Ala Glu 245 250 255 Thr Arg Ile Lys Leu Asp Leu Phe Ser Ala Leu Gly Asp Ala Lys Arg 260 265 270 Gln Leu Glu Ile Ala Gln Gly Gln Ile Leu Gln Lys Asp Gln Gly Asn 275 280 285 Gln Gly Pro Lys Thr Glu Asp Ser Arg Ser His Gly Arg His Ala Gln 290 295 300 His Asn Ile Gln Cys Arg His Gln Pro Pro Glu Pro Cys Phe Pro Pro 305 310 315 320 Leu Leu Phe Gln Ile Cys Gly Asp Gln Pro Leu Trp Thr Leu Thr Pro 325 330 335 Met Ala Ser Val Tyr Gln Pro Leu Lys Lys Leu Lys Ala Ser Leu Cys 340 345 350 Val Val Pro Lys Lys Phe Gly Leu Pro Gly Lys Ala Leu Gln Arg 355 360 365 <210> 105 <211> 548 <212> PRT <213> Homo sapiens <220> <223> Designation: HFB101-1 <400> 105 Met Asn Ile Glu Val Val Glu Val Leu Thr Leu Asn Gln Glu Val Ala 1 5 10 15 Gly Pro Arg Asn Ala Gln Ile Gln Ala Leu Tyr Ala Glu Asp Gly Ser 20 25 30 Leu Ser Ala Asp Ala Pro Ser Glu Gln Ile Gln Gln Gln Gly Lys His 35 40 45 Pro Gly Asp Pro Glu Ala Ala Arg Gln Arg Phe Arg Gln Phe Arg Tyr 50 55 60 Lys Asp Met Thr Gly Pro Arg Glu Ala Leu Asp Gln Leu Arg Glu Leu 65 70 75 80 Cys His Gln Trp Leu Gln Pro Lys Ala Arg Ser Lys Glu Gln Ile Leu 85 90 95 Glu Leu Leu Val Leu Glu Gln Phe Leu Gly Ala Leu Pro Val Lys Leu 100 105 110 Arg Thr Trp Val Glu Ser Gln His Pro Glu Asn Cys Gln Glu Val Val 115 120 125 Ala Leu Val Glu Gly Val Thr Trp Met Ser Glu Glu Glu Val Leu Pro 130 135 140 Ala Gly Gln Pro Ala Glu Gly Thr Thr Cys Cys Leu Glu Val Thr Ala 145 150 155 160 Gln Gln Glu Glu Lys Gln Glu Asp Ala Ala Ile Cys Pro Val Thr Val 165 170 175 Leu Pro Glu Glu Pro Val Thr Phe Gln Asp Val Ala Val Asp Phe Ser 180 185 190 Arg Glu Glu Trp Gly Leu Leu Gly Pro Thr Gln Arg Thr Glu Tyr Arg 195 200 205 Asp Val Met Leu Glu Thr Phe Gly His Leu Val Ser Val Gly Trp Glu 210 215 220 Thr Thr Leu Glu Asn Lys Glu Leu Ala Pro Asn Ser Asp Ile Pro Glu 225 230 235 240 Glu Glu Pro Ala Pro Ser Leu Lys Val Gln Glu Ser Ser Arg Asp Cys 245 250 255 Ala Leu Ser Ser Thr Leu Glu Asp Thr Leu Gln Gly Gly Val Gln Glu 260 265 270 Val Gln Asp Thr Val Leu Lys Gln Met Glu Ser Ala Gln Glu Lys Asp 275 280 285 Leu Pro Gln Lys Lys His Phe Asp Asn Arg Glu Ser Gln Ala Asn Ser 290 295 300 Gly Ala Leu Asp Thr Asn Gln Val Ser Leu Gln Lys Ile Asp Asn Pro 305 310 315 320 Glu Ser Gln Ala Asn Ser Gly Ala Leu Asp Thr Asn Gln Val Leu Leu 325 330 335 His Lys Ile Pro Pro Arg Lys Arg Leu Arg Lys Arg Asp Ser Gln Val 340 345 350 Lys Ser Met Lys His Asn Ser Arg Val Lys Ile His Gln Lys Ser Cys 355 360 365 Glu Arg Gln Lys Ala Lys Glu Gly Asn Gly Cys Arg Lys Thr Phe Ser 370 375 380 Arg Ser Thr Lys Gln Ile Thr Phe Ile Arg Ile His Lys Gly Ser Gln 385 390 395 400 Val Cys Arg Cys Ser Glu Cys Gly Lys Ile Phe Arg Asn Pro Arg Tyr 405 410 415 Phe Ser Val His Lys Lys Ile His Thr Gly Glu Arg Pro Tyr Val Cys 420 425 430 Gln Asp Cys Gly Lys Gly Phe Val Gln Ser Ser Ser Leu Thr Gln His 435 440 445 Gln Arg Val His Ser Gly Glu Arg Pro Phe Glu Cys Gln Glu Cys Gly 450 455 460 Arg Thr Phe Asn Asp Arg Ser Ala Ile Ser Gln His Leu Arg Thr His 465 470 475 480 Thr Gly Ala Lys Pro Tyr Lys Cys Gln Asp Cys Gly Lys Ala Phe Arg 485 490 495 Gln Ser Ser His Leu Ile Arg His Gln Arg Thr His Thr Gly Glu Arg 500 505 510 Pro Tyr Ala Cys Asn Lys Cys Gly Lys Ala Phe Thr Gln Ser Ser His 515 520 525 Leu Ile Trp His Gln Arg Thr His Asn Arg Thr Lys Arg Lys Lys Lys 530 535 540 Gln Pro Thr Ser 545 <210> 106 <211> 621 <212> PRT <213> Homo sapiens <220> <223> Designation: HFB101-2 <400> 106 Met Ala Ser Arg Leu Pro Thr Ala Trp Ser Cys Glu Pro Val Thr Phe 1 5 10 15 Glu Asp Val Thr Leu Gly Phe Thr Pro Glu Glu Trp Gly Leu Leu Asp 20 25 30 Leu Lys Gln Lys Ser Leu Tyr Arg Glu Val Met Leu Glu Asn Tyr Arg 35 40 45 Asn Leu Val Ser Val Glu Tyr Pro Glu Leu Gln Leu Asp Pro Lys Leu 50 55 60 Asp Pro Leu Pro Ala Glu Ser Pro Leu Met Asn Ile Glu Val Val Glu 65 70 75 80 Val Leu Thr Leu Asn Gln Glu Val Ala Gly Pro Arg Asn Ala Gln Ile 85 90 95 Gln Ala Leu Tyr Ala Glu Asp Gly Ser Leu Ser Ala Asp Ala Pro Ser 100 105 110 Glu Gln Val Gln Gln Gln Gly Lys His Pro Gly Asp Pro Glu Ala Ala 115 120 125 Arg Gln Arg Phe Arg Gln Phe Arg Tyr Lys Asp Met Thr Gly Pro Arg 130 135 140 Glu Ala Leu Asp Gln Leu Arg Glu Leu Cys His Gln Trp Leu Gln Pro 145 150 155 160 Lys Ala Arg Ser Lys Glu Gln Ile Leu Glu Leu Leu Val Leu Glu Gln 165 170 175 Phe Leu Gly Ala Leu Pro Val Lys Leu Arg Thr Trp Val Glu Ser Gln 180 185 190 His Pro Glu Asn Cys Gln Glu Val Val Ala Leu Val Glu Gly Val Thr 195 200 205 Trp Met Ser Glu Glu Glu Val Leu Pro Ala Gly Gln Pro Ala Glu Gly 210 215 220 Thr Thr Cys Cys Leu Glu Val Thr Ala Gln Gln Glu Glu Lys Gln Glu 225 230 235 240 Asp Ala Ala Ile Cys Pro Val Thr Val Leu Pro Glu Glu Pro Val Thr 245 250 255 Phe Gln Asp Val Ala Val Asp Phe Ser Arg Glu Glu Trp Gly Leu Leu 260 265 270 Gly Pro Thr Gln Arg Thr Glu Tyr Arg Asp Val Met Leu Glu Thr Phe 275 280 285 Gly His Leu Val Ser Val Gly Trp Glu Thr Thr Leu Glu Asn Lys Glu 290 295 300 Leu Ala Pro Asn Ser Asp Ile Pro Glu Glu Glu Pro Ala Pro Ser Leu 305 310 315 320 Lys Val Gln Glu Ser Ser Arg Asp Cys Ala Leu Ser Ser Thr Leu Glu 325 330 335 Asp Thr Leu Gln Gly Gly Val Gln Glu Val Gln Asp Thr Val Leu Lys 340 345 350 Gln Met Glu Ser Ala Gln Glu Lys Asp Leu Pro Gln Lys Lys His Phe 355 360 365 Asp Asn Arg Glu Ser Gln Ala Asn Ser Gly Ala Leu Asp Thr Asn Gln 370 375 380 Val Ser Leu Gln Lys Ile Asp Asn Pro Glu Ser Gln Ala Asn Ser Gly 385 390 395 400 Ala Leu Asp Thr Asn Gln Val Leu Leu His Lys Ile Pro Pro Arg Lys 405 410 415 Arg Leu Arg Lys Arg Asp Ser Gln Val Lys Ser Met Lys His Asn Ser 420 425 430 Arg Val Lys Ile His Gln Lys Ser Cys Glu Arg Gln Lys Ala Lys Glu 435 440 445 Gly Asn Gly Cys Arg Lys Thr Phe Ser Arg Ser Thr Lys Gln Ile Thr 450 455 460 Phe Ile Arg Ile His Lys Gly Ser Gln Val Cys Arg Cys Ser Glu Cys 465 470 475 480 Gly Lys Ile Phe Arg Asn Pro Arg Tyr Phe Ser Val His Lys Lys Ile 485 490 495 His Thr Gly Glu Arg Pro Tyr Val Cys Gln Asp Cys Gly Lys Gly Phe 500 505 510 Val Gln Ser Ser Ser Leu Thr Gln His Gln Arg Val His Ser Gly Glu 515 520 525 Arg Pro Phe Glu Cys Gln Glu Cys Gly Arg Thr Phe Asn Asp Arg Ser 530 535 540 Ala Ile Ser Gln His Leu Arg Thr His Thr Gly Ala Lys Pro Tyr Lys 545 550 555 560 Cys Gln Asp Cys Gly Lys Ala Phe Arg Gln Ser Ser His Leu Ile Arg 565 570 575 His Gln Arg Thr His Thr Gly Glu Arg Pro Tyr Ala Cys Asn Lys Cys 580 585 590 Gly Lys Ala Phe Thr Gln Ser Ser His Leu Ile Gly His Gln Arg Thr 595 600 605 His Asn Arg Thr Lys Arg Lys Lys Lys Gln Pro Thr Ser 610 615 620 <210> 107 <211> 1135 <212> PRT <213> Homo sapiens <220> <223> Designation: HFB341 <400> 107 Met Leu Leu Gly Asp Glu Lys Gln Ser Ile Val Gln Ala Lys Pro Leu 1 5 10 15 Glu Ile Ala Lys Leu Val Gln Lys Asn Pro Ala Val Gln Phe Leu Tyr 20 25 30 Gln Lys Leu Ile Gly Trp Leu Asn Glu Asp Gln Arg Lys Asp Phe Gly 35 40 45 Phe Leu Val Asp Ile Leu Tyr Ser Ala Leu Arg Cys Cys Asp Asn Asp 50 55 60 Met Glu Arg Lys Lys Val Leu Asp Asp Leu Thr Lys Val Asp Leu Lys 65 70 75 80 Trp Asn Ser Leu Leu Lys Ile Ile Glu Lys Ala Cys Pro Ser Ser Asp 85 90 95 Lys His Ala Leu Val Thr Pro Trp Leu Lys Gly Asp Ile Leu Gly Glu 100 105 110 Lys Leu Val Asn Leu Ala Asp Cys Leu Cys Asn Glu Asp Leu Glu Ser 115 120 125 Arg Val Ser Ser Glu Ser His Phe Ser Glu Arg Trp Thr Leu Leu Ser 130 135 140 Leu Val Leu Ser Gln His Val Lys Asn Asp Tyr Leu Ile Gly Asp Val 145 150 155 160 Tyr Val Glu Arg Ile Ile Val Arg Leu His Glu Thr Leu Phe Lys Thr 165 170 175 Lys Lys Leu Ser Glu Ala Glu Ser Ser Asp Ser Ser Val Ser Phe Ile 180 185 190 Cys Asp Val Ala Tyr Asn Tyr Phe Ser Ser Ala Lys Gly Cys Leu Leu 195 200 205 Met Pro Ser Ser Glu Asp Leu Leu Leu Thr Leu Phe Gln Leu Cys Ala 210 215 220 Gln Ser Lys Glu Lys Thr His Leu Pro Asp Phe Leu Ile Cys Lys Leu 225 230 235 240 Lys Asn Thr Trp Leu Ser Gly Val Asn Leu Leu Val His Gln Thr Asp 245 250 255 Ser Ser Tyr Lys Glu Ser Thr Phe Leu His Leu Ser Ala Leu Trp Leu 260 265 270 Lys Asn Gln Val Gln Ala Ser Ser Leu Asp Ile Asn Ser Leu Gln Val 275 280 285 Leu Leu Ser Ala Val Asp Asp Leu Leu Asn Thr Leu Leu Glu Ser Glu 290 295 300 Asp Ser Tyr Leu Met Gly Val Tyr Ile Gly Ser Val Met Pro Asn Asp 305 310 315 320 Ser Glu Trp Glu Lys Met Arg Gln Ser Leu Pro Met Gln Trp Leu His 325 330 335 Arg Pro Leu Leu Glu Gly Arg Leu Ser Leu Asn Tyr Glu Cys Phe Lys 340 345 350 Thr Asp Phe Lys Glu Gln Asp Ile Lys Thr Leu Pro Ser His Leu Cys 355 360 365 Thr Ser Ala Leu Leu Ser Lys Met Val Leu Ile Ala Leu Arg Lys Glu 370 375 380 Thr Val Leu Glu Asn Asn Glu Leu Glu Lys Ile Ile Ala Glu Leu Leu 385 390 395 400 Tyr Ser Leu Gln Trp Cys Glu Glu Leu Asp Asn Pro Pro Ile Phe Leu 405 410 415 Ile Gly Phe Cys Glu Ile Leu Gln Lys Met Asn Ile Thr Tyr Asp Asn 420 425 430 Leu Arg Val Leu Gly Asn Thr Ser Gly Leu Leu Gln Leu Leu Phe Asn 435 440 445 Arg Ser Arg Glu His Gly Thr Leu Trp Ser Leu Ile Ile Ala Lys Leu 450 455 460 Ile Leu Ser Arg Ser Ile Ser Ser Asp Glu Val Lys Pro His Tyr Lys 465 470 475 480 Arg Lys Glu Ser Phe Phe Pro Leu Thr Glu Gly Asn Leu His Thr Ile 485 490 495 Gln Ser Leu Cys Pro Phe Leu Ser Lys Glu Glu Lys Lys Glu Phe Ser 500 505 510 Ala Gln Cys Ile Pro Ala Leu Leu Gly Trp Thr Lys Lys Asp Leu Cys 515 520 525 Ser Thr Asn Gly Gly Phe Gly His Leu Ala Ile Phe Asn Ser Cys Leu 530 535 540 Gln Thr Lys Ser Ile Asp Asp Gly Glu Leu Leu His Gly Ile Leu Lys 545 550 555 560 Ile Ile Ile Ser Trp Lys Lys Glu His Glu Asp Ile Phe Leu Phe Ser 565 570 575 Cys Asn Leu Ser Glu Ala Ser Pro Glu Val Leu Gly Val Asn Ile Glu 580 585 590 Ile Ile Arg Phe Leu Ser Leu Phe Leu Lys Tyr Cys Ser Ser Pro Leu 595 600 605 Ala Glu Ser Glu Trp Asp Phe Ile Met Cys Ser Met Leu Ala Trp Leu 610 615 620 Glu Thr Thr Ser Glu Asn Gln Ala Leu Tyr Ser Ile Pro Leu Val Gln 625 630 635 640 Leu Phe Ala Cys Val Ser Cys Asp Leu Ala Cys Asp Leu Ser Ala Phe 645 650 655 Phe Asp Ser Thr Thr Leu Asp Thr Ile Gly Asn Leu Pro Val Asn Leu 660 665 670 Ile Ser Glu Trp Lys Glu Phe Phe Ser Gln Gly Ile His Ser Leu Leu 675 680 685 Leu Pro Ile Leu Val Thr Val Thr Gly Glu Asn Lys Asp Val Ser Glu 690 695 700 Thr Ser Phe Gln Asn Ala Met Leu Lys Pro Met Cys Glu Thr Leu Thr 705 710 715 720 Tyr Ile Ser Lys Glu Gln Leu Leu Ser His Lys Leu Pro Ala Arg Leu 725 730 735 Val Ala Asp Gln Lys Thr Asn Leu Pro Glu Tyr Leu Gln Thr Leu Leu 740 745 750 Asn Thr Leu Ala Pro Leu Leu Leu Phe Arg Ala Arg Pro Val Gln Ile 755 760 765 Ala Val Tyr His Met Leu Tyr Lys Leu Met Pro Glu Leu Pro Gln Tyr 770 775 780 Asp Gln Asp Asn Leu Lys Ser Tyr Gly Asp Glu Glu Glu Glu Pro Ala 785 790 795 800 Leu Ser Pro Pro Ala Ala Leu Met Ser Leu Leu Ser Ile Gln Glu Asp 805 810 815 Leu Leu Glu Asn Val Leu Gly Cys Ile Pro Val Gly Gln Ile Val Thr 820 825 830 Ile Lys Pro Leu Ser Glu Asp Phe Cys Tyr Val Leu Gly Tyr Leu Leu 835 840 845 Thr Trp Lys Leu Ile Leu Thr Phe Phe Lys Ala Ala Ser Ser Gln Leu 850 855 860 Arg Ala Leu Tyr Ser Met Tyr Leu Arg Lys Thr Lys Ser Leu Asn Lys 865 870 875 880 Leu Leu Tyr His Leu Phe Arg Leu Met Pro Glu Asn Pro Thr Tyr Ala 885 890 895 Glu Thr Ala Val Glu Val Pro Asn Lys Asp Pro Lys Thr Phe Phe Thr 900 905 910 Glu Glu Leu Gln Leu Ser Ile Arg Glu Thr Thr Met Leu Pro Tyr His 915 920 925 Ile Pro His Leu Ala Cys Ser Val Tyr His Met Thr Leu Lys Asp Leu 930 935 940 Pro Ala Met Val Arg Leu Trp Trp Asn Ser Ser Glu Lys Arg Val Phe 945 950 955 960 Asn Ile Val Asp Arg Phe Thr Ser Lys Tyr Val Ser Ser Val Leu Ser 965 970 975 Phe Gln Glu Ile Ser Ser Val Gln Thr Ser Thr Gln Leu Phe Asn Gly 980 985 990 Met Thr Val Lys Ala Arg Ala Thr Thr Arg Glu Val Met Ala Thr Tyr 995 1000 1005 Thr Ile Glu Asp Ile Val Ile Glu Leu Ile Ile Gln Leu Pro Ser Asn 1010 1015 1020 Tyr Pro Leu Gly Ser Ile Ile Val Glu Ser Gly Lys Arg Val Gly Val 1025 1030 1035 1040 Ala Val Gln Gln Trp Arg Asn Trp Met Leu Gln Leu Ser Thr Tyr Leu 1045 1050 1055 Thr His Gln Asn Gly Ser Ile Met Glu Gly Leu Ala Leu Trp Lys Asn 1060 1065 1070 Asn Val Asp Lys Arg Phe Glu Gly Val Glu Asp Cys Met Ile Cys Phe 1075 1080 1085 Ser Val Ile His Gly Phe Asn Tyr Ser Leu Pro Lys Lys Ala Cys Arg 1090 1095 1100 Thr Cys Lys Lys Lys Phe His Ser Ala Cys Leu Tyr Lys Trp Phe Thr 1105 1110 1115 1120 Ser Ser Asn Lys Ser Thr Cys Pro Leu Cys Arg Glu Thr Phe Phe 1125 1130 1135

【図面の簡単な説明】

【図１】プラスミド「pLexAD」を示す。

【図２】プラスミド「pLexADrev」を示す。

【図３】プラスミド「pRS1F」を示す。

【図４】プラスミド「pRS3F」を示す。

【図５】被検ペプチドとの融合に用いる転写因子の核移
行能のアッセイを示す。

【図６】被検ペプチドを融合した転写因子の核移行能の
アッセイを示す。

【図７】プラスミド「pNS」を示す。

【図８】NTT法により単離した21の遺伝子の核移行活性
を検出した結果を示す顕微鏡写真である。図中の番号は
表1および表2における遺伝子クローンの順番（表1にお
ける最初のクローンを1として、表2における最後のクロ
ーンを21とした）に対応する。

Claims (7)

【特許請求の範囲】
1. 【請求項１】 配列番号：１から２１、１０１から１０
   ７のいずれかに記載のアミノ酸配列を含み、核移行活性
   を有するペプチド。
2. 【請求項２】 配列番号：１から２１、１０１から１０
   ７のいずれかに記載のアミノ酸配列において1若しくは
   複数のアミノ酸が置換、欠失、および／若しくは付加し
   たアミノ酸配列を含み、核移行活性を有するペプチド。
3. 【請求項３】 核へ輸送するための所望のペプチドを含
   む融合ペプチドである、請求項１または２に記載のペプ
   チド。
4. 【請求項４】 請求項１から３のいずれかに記載のペプ
   チドをコードするDNA。
5. 【請求項５】 請求項４に記載のDNAを含むベクター。
6. 【請求項６】 請求項５に記載のベクターを保持する形
   質転換細胞。
7. 【請求項７】 請求項６に記載の形質転換細胞を培養す
   る工程を含む、請求項１または２に記載のペプチドの製
   造方法。