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IT1311896B1 - METHOD FOR DETERMINING SEQUENCE ALTERATIONS OF MOLECOLEDI DNA BY MULTIPLE-DYE CFLP (MD-CFLP). - Google Patents

METHOD FOR DETERMINING SEQUENCE ALTERATIONS OF MOLECOLEDI DNA BY MULTIPLE-DYE CFLP (MD-CFLP).

Info

Publication number
IT1311896B1
IT1311896B1 IT1999MI000512A ITMI990512A IT1311896B1 IT 1311896 B1 IT1311896 B1 IT 1311896B1 IT 1999MI000512 A IT1999MI000512 A IT 1999MI000512A IT MI990512 A ITMI990512 A IT MI990512A IT 1311896 B1 IT1311896 B1 IT 1311896B1
Authority
IT
Italy
Prior art keywords
cflp
molecoledi
dye
dna
determining sequence
Prior art date
Application number
IT1999MI000512A
Other languages
Italian (it)
Inventor
Daniele Calistri
Laura Cortesi
Original Assignee
Istituto Oncologico Romagnolo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Istituto Oncologico Romagnolo filed Critical Istituto Oncologico Romagnolo
Priority to IT1999MI000512A priority Critical patent/IT1311896B1/en
Priority to EP00918777A priority patent/EP1159456A2/en
Priority to AU39615/00A priority patent/AU3961500A/en
Priority to PCT/EP2000/002054 priority patent/WO2000055360A2/en
Publication of ITMI990512A1 publication Critical patent/ITMI990512A1/en
Application granted granted Critical
Publication of IT1311896B1 publication Critical patent/IT1311896B1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
IT1999MI000512A 1999-03-12 1999-03-12 METHOD FOR DETERMINING SEQUENCE ALTERATIONS OF MOLECOLEDI DNA BY MULTIPLE-DYE CFLP (MD-CFLP). IT1311896B1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
IT1999MI000512A IT1311896B1 (en) 1999-03-12 1999-03-12 METHOD FOR DETERMINING SEQUENCE ALTERATIONS OF MOLECOLEDI DNA BY MULTIPLE-DYE CFLP (MD-CFLP).
EP00918777A EP1159456A2 (en) 1999-03-12 2000-03-09 Method for the determination of alterations in the sequence of dna molecules using multiple-dye cflp (md-cflp)
AU39615/00A AU3961500A (en) 1999-03-12 2000-03-09 Method for the determination of alterations in the sequence of dna molecules using multiple-dye cflp (md-cflp)
PCT/EP2000/002054 WO2000055360A2 (en) 1999-03-12 2000-03-09 Method for the determination of alterations in the sequence of dna molecules using multiple-dye cflp (md-cflp)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT1999MI000512A IT1311896B1 (en) 1999-03-12 1999-03-12 METHOD FOR DETERMINING SEQUENCE ALTERATIONS OF MOLECOLEDI DNA BY MULTIPLE-DYE CFLP (MD-CFLP).

Publications (2)

Publication Number Publication Date
ITMI990512A1 ITMI990512A1 (en) 2000-09-12
IT1311896B1 true IT1311896B1 (en) 2002-03-20

Family

ID=11382265

Family Applications (1)

Application Number Title Priority Date Filing Date
IT1999MI000512A IT1311896B1 (en) 1999-03-12 1999-03-12 METHOD FOR DETERMINING SEQUENCE ALTERATIONS OF MOLECOLEDI DNA BY MULTIPLE-DYE CFLP (MD-CFLP).

Country Status (4)

Country Link
EP (1) EP1159456A2 (en)
AU (1) AU3961500A (en)
IT (1) IT1311896B1 (en)
WO (1) WO2000055360A2 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843654A (en) * 1992-12-07 1998-12-01 Third Wave Technologies, Inc. Rapid detection of mutations in the p53 gene
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US6403303B1 (en) * 1996-05-14 2002-06-11 Visible Genetics Inc. Method and reagents for testing for mutations in the BRCA1 gene
CA2289872C (en) * 1997-05-05 2007-07-31 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides

Also Published As

Publication number Publication date
AU3961500A (en) 2000-10-04
ITMI990512A1 (en) 2000-09-12
WO2000055360A3 (en) 2000-12-28
WO2000055360A2 (en) 2000-09-21
EP1159456A2 (en) 2001-12-05

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