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IT1230139B - Pertussis toxin mutants and bordetella strains producing toxin - Google Patents

Pertussis toxin mutants and bordetella strains producing toxin

Info

Publication number
IT1230139B
IT1230139B IT8920341A IT2034189A IT1230139B IT 1230139 B IT1230139 B IT 1230139B IT 8920341 A IT8920341 A IT 8920341A IT 2034189 A IT2034189 A IT 2034189A IT 1230139 B IT1230139 B IT 1230139B
Authority
IT
Italy
Prior art keywords
toxin
bordetella
bordetella strains
mutants
ile88
Prior art date
Application number
IT8920341A
Other languages
Italian (it)
Other versions
IT8920341A0 (en
Inventor
Mariagrazia Pizza
Antonello Covacci
Rino Rappuoli
Original Assignee
Sclavo Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sclavo Spa filed Critical Sclavo Spa
Publication of IT8920341A0 publication Critical patent/IT8920341A0/en
Priority to IT8920341A priority Critical patent/IT1230139B/en
Priority to EP90107892A priority patent/EP0396964B1/en
Priority to DE69022106T priority patent/DE69022106T2/en
Priority to DK90107892.3T priority patent/DK0396964T3/en
Priority to AT90107892T priority patent/ATE127347T1/en
Priority to ES90107892T priority patent/ES2078258T3/en
Priority to CA002015677A priority patent/CA2015677C/en
Priority to MX2050290A priority patent/MX20502A/en
Priority to JP2111865A priority patent/JP2655583B2/en
Application granted granted Critical
Publication of IT1230139B publication Critical patent/IT1230139B/en
Priority to US08/261,691 priority patent/US7427404B1/en
Priority to US08/431,673 priority patent/US7666436B1/en
Priority to GR950403403T priority patent/GR3018285T3/en
Priority to HK17096A priority patent/HK17096A/en
Priority to JP8191142A priority patent/JP2852025B2/en

Links

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The toxin mutants (A) have at least one of the amino acid (AA) residues Glu129, Asp11, Trp26, Arg9, Phe50, Asp1, Arg13, Tyr130, Gly86, Ile88, Tyr89, Tyr8, Gly44, Thr53 and Gly80 of subunit S1 deleted and substd. by a natural AA. Also claimed are (a) Bordetella strains comprising (i) the gene encoding the wild type pertussin toxin; (ii) an expression and secretion regulation sequence; with the mutated subunit S1. E.g. Bordetella pertussis (W28)PT-9K/129G ATCC53894; B.parapertussis PT-129G ATCC 53892, PT-26l/129G ATCC 53893; B.bronchiseptica; (b) an immunogenic formulation. Details for obtg. (A) and Bordetella strains are given. Six specific examples of (A) are given, e.g. PT-13l/129G and PT-88E/89S have AA Glu129 + Arg13 and Ile88 + Tyr89 substd. by Gly+Ile and Glu+Ser respectively. (A), stable at 37 deg.C, have reduced or no mitogenicity of haemagglutinin activity and are obtd. by 0.035% and 0.42% formaldehyde treatment.
IT8920341A 1989-04-28 1989-04-28 Pertussis toxin mutants and bordetella strains producing toxin IT1230139B (en)

Priority Applications (14)

Application Number Priority Date Filing Date Title
IT8920341A IT1230139B (en) 1989-04-28 1989-04-28 Pertussis toxin mutants and bordetella strains producing toxin
EP90107892A EP0396964B1 (en) 1989-04-28 1990-04-25 Pertussis toxin mutants, bordetella strains capable of producing such mutants and their use in the development of antipertussis vaccines
DE69022106T DE69022106T2 (en) 1989-04-28 1990-04-25 Pertussis toxin mutants, the same producing Bordetella strains and their use as a vaccine against pertussis.
DK90107892.3T DK0396964T3 (en) 1989-04-28 1990-04-25 Pertussis toxin mutants, Bordetella strains capable of producing such mutants, and their use in the development of antipertussis vaccines
AT90107892T ATE127347T1 (en) 1989-04-28 1990-04-25 PERTUSSISTOXIN MUTANTS, BORDETELLA STRAINS PRODUCING THE SAME, AND THEIR USE AS A VACCINE AGAINST PERTUSSIS.
ES90107892T ES2078258T3 (en) 1989-04-28 1990-04-25 PERTUSSIC TOXIN MUTANTS, BORDETELLA STRAINS ABLE TO PRODUCE SUCH MUTANTS AND THEIR USE IN THE DEVELOPMENT OF ANTIPERTUSSIC VACCINES.
CA002015677A CA2015677C (en) 1989-04-28 1990-04-27 Pertussis toxin mutants, bordetella strains capable of producing such mutants and their use in the development of antipertussis vaccines
MX2050290A MX20502A (en) 1989-04-28 1990-04-27 MUTANTS OF TOSPHERIN TOXIN IMMUNOLOGICALLY ACTIVE AND WITH A REDUCED OR NO TOXICITY AND PROCEDURE FOR ITS PREPARATION.
JP2111865A JP2655583B2 (en) 1989-04-28 1990-05-01 Novel pertussis toxin variants, Bordetella strains capable of producing such variants and their use in developing anti-pertussis vaccines
US08/261,691 US7427404B1 (en) 1989-04-28 1994-06-17 Pertussis toxin mutants, bordetella strains capable of producing such mutants and their use in the development of antipertussis vaccines
US08/431,673 US7666436B1 (en) 1989-04-28 1995-05-02 Pertussis toxin mutants, Bordetella strains capable of producing such mutants and their use in the development of antipertussis vaccines
GR950403403T GR3018285T3 (en) 1989-04-28 1995-12-05 Pertussis toxin mutants, bordetella strains capable of producing such mutants and their use in the development of antipertussis vaccines.
HK17096A HK17096A (en) 1989-04-28 1996-02-01 Pertussis toxin mutants bordetella strains capable of producing such mutants and their use in the development of antipertussis vaccines
JP8191142A JP2852025B2 (en) 1989-04-28 1996-07-19 Novel pertussis toxin mutants, Bordetella strains capable of producing such mutants and their use in the development of anti-pertussis vaccines

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT8920341A IT1230139B (en) 1989-04-28 1989-04-28 Pertussis toxin mutants and bordetella strains producing toxin

Publications (2)

Publication Number Publication Date
IT8920341A0 IT8920341A0 (en) 1989-04-28
IT1230139B true IT1230139B (en) 1991-10-14

Family

ID=11165868

Family Applications (1)

Application Number Title Priority Date Filing Date
IT8920341A IT1230139B (en) 1989-04-28 1989-04-28 Pertussis toxin mutants and bordetella strains producing toxin

Country Status (2)

Country Link
IT (1) IT1230139B (en)
MX (1) MX20502A (en)

Also Published As

Publication number Publication date
MX20502A (en) 1994-02-28
IT8920341A0 (en) 1989-04-28

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TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19970423