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IT1153912B - Metodo e reagenti per l'uso di beta-galattosidasi come marcante in immunocitochimica - Google Patents

Metodo e reagenti per l'uso di beta-galattosidasi come marcante in immunocitochimica

Info

Publication number
IT1153912B
IT1153912B IT24880/82A IT2488082A IT1153912B IT 1153912 B IT1153912 B IT 1153912B IT 24880/82 A IT24880/82 A IT 24880/82A IT 2488082 A IT2488082 A IT 2488082A IT 1153912 B IT1153912 B IT 1153912B
Authority
IT
Italy
Prior art keywords
immunocytochemistry
galactosidase
reagents
marker
beta
Prior art date
Application number
IT24880/82A
Other languages
English (en)
Other versions
IT8224880A0 (it
IT8224880A1 (it
Inventor
Giorgio Chieregatti
Enzo Murador
Original Assignee
Erba Farmitalia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Erba Farmitalia filed Critical Erba Farmitalia
Publication of IT8224880A0 publication Critical patent/IT8224880A0/it
Publication of IT8224880A1 publication Critical patent/IT8224880A1/it
Application granted granted Critical
Publication of IT1153912B publication Critical patent/IT1153912B/it

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/535Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/50Indoles
    • C12Q2334/525-Bromo-4-chloro-3-indolyl, i.e. BCI
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/924Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
IT24880/82A 1982-01-20 1982-12-21 Metodo e reagenti per l'uso di beta-galattosidasi come marcante in immunocitochimica IT1153912B (it)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8201547 1982-01-20

Publications (3)

Publication Number Publication Date
IT8224880A0 IT8224880A0 (it) 1982-12-21
IT8224880A1 IT8224880A1 (it) 1984-06-21
IT1153912B true IT1153912B (it) 1987-01-21

Family

ID=10527737

Family Applications (1)

Application Number Title Priority Date Filing Date
IT24880/82A IT1153912B (it) 1982-01-20 1982-12-21 Metodo e reagenti per l'uso di beta-galattosidasi come marcante in immunocitochimica

Country Status (3)

Country Link
DE (1) DE3300961A1 (it)
FR (1) FR2520121B1 (it)
IT (1) IT1153912B (it)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4218539A (en) * 1978-03-24 1980-08-19 Weltman Joel K Enzyme conjugates and method of preparation and use
GB2098730B (en) * 1981-04-21 1985-02-06 Welsh Nat School Med Immunolocalisation

Also Published As

Publication number Publication date
IT8224880A0 (it) 1982-12-21
FR2520121B1 (fr) 1985-08-23
FR2520121A1 (fr) 1983-07-22
IT8224880A1 (it) 1984-06-21
DE3300961A1 (de) 1983-07-28

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