IN2012DN00899A - - Google Patents
Info
- Publication number
- IN2012DN00899A IN2012DN00899A IN899DEN2012A IN2012DN00899A IN 2012DN00899 A IN2012DN00899 A IN 2012DN00899A IN 899DEN2012 A IN899DEN2012 A IN 899DEN2012A IN 2012DN00899 A IN2012DN00899 A IN 2012DN00899A
- Authority
- IN
- India
- Prior art keywords
- culture system
- cell culture
- cell
- continuous
- less
- Prior art date
Links
- 238000004113 cell culture Methods 0.000 abstract 7
- 210000004027 cell Anatomy 0.000 abstract 6
- 241000700605 Viruses Species 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 3
- 210000004962 mammalian cell Anatomy 0.000 abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 238000010790 dilution Methods 0.000 abstract 2
- 239000012895 dilution Substances 0.000 abstract 2
- 230000014759 maintenance of location Effects 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000010412 perfusion Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
- C12M3/006—Cell injection or fusion devices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0056—Xeno-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6489—Metalloendopeptidases (3.4.24)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/98—Xeno-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/00051—Methods of production or purification of viral material
- C12N2710/00052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d-1, and a cell density of less than about 2X107cell/mL. Also described herein is a method for producing a polypeptide and/or virus of interest in a continuous cell culture, the method comprising culturing mammalian cells expressing the polypeptide and/or virus of interest in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2d-1, and a cell density of less than about 2X107 cell/mL; and recovering the polypeptide and/or virus of interest from medium of the cell culture system.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23031309P | 2009-07-31 | 2009-07-31 | |
| PCT/EP2010/061191 WO2011012725A1 (en) | 2009-07-31 | 2010-08-02 | Method of producing a polypeptide or virus of interest in a continuous cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2012DN00899A true IN2012DN00899A (en) | 2015-04-03 |
Family
ID=42668436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN899DEN2012 IN2012DN00899A (en) | 2009-07-31 | 2010-08-02 |
Country Status (22)
| Country | Link |
|---|---|
| US (4) | US8580554B2 (en) |
| EP (2) | EP2459697B1 (en) |
| JP (2) | JP5808742B2 (en) |
| KR (2) | KR101752789B1 (en) |
| CN (1) | CN102549142B (en) |
| AU (1) | AU2010277490B2 (en) |
| BR (1) | BR112012002137B8 (en) |
| CA (1) | CA2769354A1 (en) |
| CO (1) | CO6491119A2 (en) |
| DK (1) | DK2459697T3 (en) |
| EA (1) | EA022219B1 (en) |
| ES (1) | ES2705206T3 (en) |
| HR (1) | HRP20182165T1 (en) |
| IN (1) | IN2012DN00899A (en) |
| MX (2) | MX2012001274A (en) |
| NZ (1) | NZ597742A (en) |
| PL (1) | PL2459697T3 (en) |
| PT (1) | PT2459697T (en) |
| SG (1) | SG178176A1 (en) |
| SI (1) | SI2459697T1 (en) |
| TR (1) | TR201900213T4 (en) |
| WO (1) | WO2011012725A1 (en) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112013000515B1 (en) | 2010-07-08 | 2021-11-03 | Takeda Pharmaceutical Company Limited | METHOD FOR PRODUCING A RECOMBINANT VON WILLEBRAND FACTOR COMPOSITION (RVWF) |
| PL2665806T3 (en) * | 2011-01-17 | 2021-12-27 | F.Hoffmann-La Roche Ag | Separation apparatus |
| RU2600886C2 (en) * | 2011-05-13 | 2016-10-27 | Октафарма Аг | Method of increasing productivity of eucaryotic cells in production of recombinant fviii |
| KR102108663B1 (en) | 2011-07-01 | 2020-05-07 | 암젠 인크 | Mammalian cell culture |
| CN203129697U (en) | 2013-02-05 | 2013-08-14 | 客贝利(厦门)休闲用品有限公司 | Tent frame pole |
| ES2663815T3 (en) | 2011-11-24 | 2018-04-17 | Genethon | Production system of a scalable lentiviral vector compatible with industrial pharmaceutical applications |
| US9574167B2 (en) | 2013-02-15 | 2017-02-21 | Pharyx, Inc. | Methods and apparatus for independent control of product and reactant concentrations |
| IL274646B (en) | 2013-03-08 | 2022-09-01 | Genzyme Corp | Integrated continuous production of medicinal substances from medical protein |
| US9217168B2 (en) | 2013-03-14 | 2015-12-22 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
| WO2014146933A1 (en) * | 2013-03-19 | 2014-09-25 | Cmc Biologics A/S | A method for producing a product (e.g. polypeptide) in a continuous cell culture fermentation process. |
| CN105473712B (en) * | 2013-08-30 | 2022-05-27 | 葛兰素史密丝克莱恩生物有限公司 | Large scale production of viruses in cell culture |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| TWI709569B (en) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | Sterile chromatography resin and use thereof in manufacturing processes |
| TWI709570B (en) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | Sterile chromatography and manufacturing processes |
| JP2017509658A (en) | 2014-04-01 | 2017-04-06 | アドヴァンテック・バイオサイエンス・ファルマスーティカ・リミターダ | Stabilization of factor VIII without the use of calcium additives |
| WO2015149143A2 (en) | 2014-04-01 | 2015-10-08 | Advantech Bioscience Farmacêutica Ltda. | Stable factor viii formulations with low sugar-glycine |
| CN106414719B (en) * | 2014-06-13 | 2020-02-14 | 杰特有限公司 | Improved production of recombinant von Willebrand factor in a bioreactor |
| EP3408371A1 (en) * | 2016-01-26 | 2018-12-05 | Boehringer Ingelheim International Gmbh | Linked perfusion to continuous-flow stirred-tank reactor cell culture system |
| CN109072154A (en) * | 2016-04-15 | 2018-12-21 | 勃林格殷格翰国际公司 | Cell retaining device and method |
| EP3559203B1 (en) * | 2016-12-21 | 2020-12-02 | H. Hoffnabb-La Roche Ag | Growth control of eukaryotic cells |
| IL307155B1 (en) | 2018-05-01 | 2025-07-01 | Amgen Inc | Antibodies with modulated glycan profiles |
| WO2019239780A1 (en) * | 2018-06-15 | 2019-12-19 | 富士フイルム株式会社 | Cell culture method, cell culture device, and method for producing product |
| BR112021003420A2 (en) | 2018-08-31 | 2021-05-18 | Genzyme Corporation | sterile chromatographic resin and its use in manufacturing processes |
| CN113355297A (en) * | 2021-06-23 | 2021-09-07 | 吉林冠界生物技术有限公司 | Method for producing recombinant avian influenza virus by perfusion culture of full-suspension MDCK cells |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5019512A (en) | 1989-03-17 | 1991-05-28 | Baxter International Inc. | Spin filter for removing substantially cell-free culture medium from suspension cell culture system |
| US5626734A (en) | 1995-08-18 | 1997-05-06 | University Technologies International, Inc. | Filter for perfusion cultures of animal cells and the like |
| HUP0301245A3 (en) | 2000-10-02 | 2005-12-28 | Novo Nordisk Healthcare Ag | Tmethod for the production of vitamin k-dependent proteins |
| NZ533084A (en) * | 2001-11-28 | 2006-02-24 | Sandoz Ag | Cell culture process |
| TWI384069B (en) | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | Production of polypeptides |
| DK1902141T3 (en) | 2005-06-17 | 2012-05-07 | Baxter Int | ADAMTS 13-containing compositions with thrombolytic effect |
| PT1974014T (en) | 2006-01-04 | 2017-05-26 | Baxalta Inc | Oligopeptide-free cell culture media |
| MX343269B (en) * | 2007-12-27 | 2016-10-31 | Baxter Int | Cell culture processes. |
-
2010
- 2010-07-30 US US12/847,921 patent/US8580554B2/en active Active
- 2010-08-02 CA CA2769354A patent/CA2769354A1/en not_active Abandoned
- 2010-08-02 SI SI201031835T patent/SI2459697T1/en unknown
- 2010-08-02 KR KR1020127004996A patent/KR101752789B1/en active Active
- 2010-08-02 BR BR112012002137A patent/BR112012002137B8/en active IP Right Grant
- 2010-08-02 TR TR2019/00213T patent/TR201900213T4/en unknown
- 2010-08-02 WO PCT/EP2010/061191 patent/WO2011012725A1/en active Application Filing
- 2010-08-02 EP EP10737916.6A patent/EP2459697B1/en active Active
- 2010-08-02 CN CN201080036754.2A patent/CN102549142B/en active Active
- 2010-08-02 DK DK10737916.6T patent/DK2459697T3/en active
- 2010-08-02 PL PL10737916T patent/PL2459697T3/en unknown
- 2010-08-02 EA EA201270213A patent/EA022219B1/en not_active IP Right Cessation
- 2010-08-02 AU AU2010277490A patent/AU2010277490B2/en active Active
- 2010-08-02 IN IN899DEN2012 patent/IN2012DN00899A/en unknown
- 2010-08-02 JP JP2012522190A patent/JP5808742B2/en active Active
- 2010-08-02 EP EP18199855.0A patent/EP3480292A1/en not_active Withdrawn
- 2010-08-02 MX MX2012001274A patent/MX2012001274A/en active IP Right Grant
- 2010-08-02 KR KR1020177017512A patent/KR101885046B1/en active Active
- 2010-08-02 PT PT10737916T patent/PT2459697T/en unknown
- 2010-08-02 ES ES10737916T patent/ES2705206T3/en active Active
- 2010-08-02 NZ NZ597742A patent/NZ597742A/en unknown
- 2010-08-02 HR HRP20182165TT patent/HRP20182165T1/en unknown
- 2010-08-02 SG SG2012006599A patent/SG178176A1/en unknown
-
2012
- 2012-01-27 MX MX2014008515A patent/MX353610B/en unknown
- 2012-02-06 CO CO12020130A patent/CO6491119A2/en unknown
-
2013
- 2013-10-10 US US14/050,710 patent/US9650612B2/en active Active
-
2015
- 2015-01-14 JP JP2015004718A patent/JP6177260B2/en active Active
-
2017
- 2017-04-12 US US15/486,181 patent/US10221400B2/en active Active
-
2019
- 2019-01-18 US US16/252,216 patent/US10745672B2/en active Active
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