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HUP0203537A2 - Methods of producing a target molecule in a transgenic animal and purification of the target molecule - Google Patents

Methods of producing a target molecule in a transgenic animal and purification of the target molecule

Info

Publication number
HUP0203537A2
HUP0203537A2 HU0203537A HUP0203537A HUP0203537A2 HU P0203537 A2 HUP0203537 A2 HU P0203537A2 HU 0203537 A HU0203537 A HU 0203537A HU P0203537 A HUP0203537 A HU P0203537A HU P0203537 A2 HUP0203537 A2 HU P0203537A2
Authority
HU
Hungary
Prior art keywords
target molecule
purification
methods
transgenic animal
producing
Prior art date
Application number
HU0203537A
Other languages
Hungarian (hu)
Inventor
Yann Echelard
Scott P. Fulton
Harry Meade
Original Assignee
Genzyme Transgenics Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Transgenics Corporation filed Critical Genzyme Transgenics Corporation
Publication of HUP0203537A2 publication Critical patent/HUP0203537A2/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A találmány tárgyát biológiai rendszerekben található célmolekulákelőállítására és tisztítására szolgáló rendszerek és eljárásokképezik. Közelebbről, a találmány tárgyát megköthető epitópottartalmazó célpolipeptid tisztítására szolgáló, egy vagy többmultivalens kötőpolipeptidet és mátrixot alkalmazó,transzgénikusállat-alapú rendszerek és eljárások képezik. A találmánytárgyát képezik továbbá maguk a transzgenikus állatok is. ÓThe subject of the invention is systems and methods for the production and purification of target molecules in biological systems. More specifically, the subject of the invention is transgenic animal-based systems and methods for the purification of a target polypeptide containing a bindable epitope, using one or more multivalent binding polypeptides and a matrix. The invention also includes the transgenic animals themselves. HE

HU0203537A 1999-10-14 2000-10-16 Methods of producing a target molecule in a transgenic animal and purification of the target molecule HUP0203537A2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15974899P 1999-10-14 1999-10-14
US20466200P 2000-05-17 2000-05-17
PCT/US2000/028589 WO2001026455A1 (en) 1999-10-14 2000-10-16 Methods of producing a target molecule in a transgenic animal and purification of the target molecule

Publications (1)

Publication Number Publication Date
HUP0203537A2 true HUP0203537A2 (en) 2003-02-28

Family

ID=26856252

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0203537A HUP0203537A2 (en) 1999-10-14 2000-10-16 Methods of producing a target molecule in a transgenic animal and purification of the target molecule

Country Status (14)

Country Link
EP (1) EP1220606A4 (en)
JP (1) JP2003511048A (en)
KR (1) KR100772465B1 (en)
CN (1) CN1322003C (en)
AU (1) AU781944B2 (en)
BR (1) BR0014860A (en)
CA (1) CA2382741A1 (en)
HU (1) HUP0203537A2 (en)
IL (1) IL149003A0 (en)
MX (1) MXPA02003702A (en)
NO (1) NO20021686L (en)
NZ (1) NZ518263A (en)
RU (1) RU2002112460A (en)
WO (1) WO2001026455A1 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6800462B2 (en) * 2001-09-10 2004-10-05 Abgenomics Corporation Production of recombinant proteins in vivo and use for generating antibodies
US20030049694A1 (en) * 2001-09-10 2003-03-13 Chung-Hsiun Wu Production of fusion proteins and use for identifying binding molecules
CN1283785C (en) * 2002-05-20 2006-11-08 上海杰隆生物工程股份有限公司 Method of producing human forcing erythrogenin using transgene animal mammary gland
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
WO2007052096A2 (en) * 2005-06-29 2007-05-10 Cameron Malcolm Lang Clokie Production of bone morphogenic proteins (bmps) in transgenic mammals
US20090228999A1 (en) * 2007-06-13 2009-09-10 Sterrenbeld Biotechnologie North America, Inc. Process of Making Transgenic Mammals That Produce Exogenous Proteins in Milk and Transgenic Mammals Produced Thereby
US9150880B2 (en) 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
WO2010118360A1 (en) 2009-04-09 2010-10-14 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Production of proteins using transposon-based vectors
EP2793936A4 (en) * 2011-12-19 2015-05-27 Lfb Usa Inc Recombinant human alpha-1-antitrypsin for the treatment of inflammatory disorders
BR112015019341A2 (en) 2013-02-13 2017-08-22 Lab Francais Du Fractionnement ANTI-TNF-ALPHA ANTIBODY, COMPOSITION COMPRISING THE ANTIBODY, METHOD FOR PRODUCING A POPULATION OF ANTIBODIES, MAMMARY GLAND EPITHELIAL CELLS, TRANSGENIC NON-HUMAN MAMMAL, AND, MONOCLONAL ANTI-TNF ANTIBODY COMPOSITION
CN105263319A (en) 2013-02-13 2016-01-20 法国化学与生物科技实验室 Proteins with modified glycosylation and methods of production thereof
KR20160029840A (en) 2013-07-05 2016-03-15 라보라토이레 프란카이즈 듀 프락티온네먼트 에트 데스 바이오테크놀로지스 Affinity chromatography matrix
FR3025515B1 (en) 2014-09-05 2016-09-09 Lab Francais Du Fractionnement PROCESS FOR PURIFYING MONOCLONAL ANTIBODY
FR3034420A1 (en) 2015-03-31 2016-10-07 Lab Francais Du Fractionnement ANTI-CD303 MONOCLONAL ANTIBODIES
FR3038517B1 (en) 2015-07-06 2020-02-28 Laboratoire Francais Du Fractionnement Et Des Biotechnologies USE OF MODIFIED FC FRAGMENTS IN IMMUNOTHERAPY
JP6712125B2 (en) * 2015-09-04 2020-06-17 株式会社フジクラ Connection structure of oxide superconducting wire and method for manufacturing the same
FR3060394B1 (en) 2016-12-16 2019-05-24 Laboratoire Francais Du Fractionnement Et Des Biotechnologies COMBINATION OF ANTI-CD303 AND ANTI-AMHRII ANTIBODIES
FR3060395B1 (en) 2016-12-16 2019-05-24 Laboratoire Francais Du Fractionnement Et Des Biotechnologies COMBINATION OF ANTI-CD303 AND ANTI-HER2 ANTIBODIES
FR3076294B1 (en) 2017-12-29 2022-01-28 Lab Francais Du Fractionnement METHOD FOR PURIFYING ANTIBODIES FROM RAW MILK
IL313752A (en) 2021-12-20 2024-08-01 Vetoquinol Sa Anti-canine interleukine-31-receptor a (il-31ra) antibodies and the uses thereof
WO2024165823A2 (en) 2023-02-09 2024-08-15 Commissariat A L'energie Atomique Et Aux Energies Alternatives Mutant fab fragment for obtaining site-specific mono-or bifunctionalised conjugates
WO2025114614A1 (en) 2023-11-30 2025-06-05 Vetoquinol Sa Anti-canine interleukine-4-receptor alpha (il-4rα) antibodies and the uses thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK580789D0 (en) * 1989-11-20 1989-11-20 Novo Nordisk As PROCEDURE FOR PURIFICATION OF POLYPEPTIDES
US5639940A (en) * 1994-03-03 1997-06-17 Pharmaceutical Proteins Ltd. Production of fibrinogen in transgenic animals
CN1114357A (en) * 1994-07-01 1996-01-03 唐勇炜 Process for producing recombinative polypeptide using transgene animal

Also Published As

Publication number Publication date
JP2003511048A (en) 2003-03-25
NO20021686D0 (en) 2002-04-10
CN1387399A (en) 2002-12-25
AU8025400A (en) 2001-04-23
EP1220606A4 (en) 2004-04-14
IL149003A0 (en) 2002-11-10
NZ518263A (en) 2004-03-26
MXPA02003702A (en) 2002-09-18
WO2001026455A1 (en) 2001-04-19
CN1322003C (en) 2007-06-20
EP1220606A1 (en) 2002-07-10
KR20030022759A (en) 2003-03-17
NO20021686L (en) 2002-06-12
CA2382741A1 (en) 2001-04-19
AU781944B2 (en) 2005-06-23
KR100772465B1 (en) 2007-11-02
RU2002112460A (en) 2004-02-27
BR0014860A (en) 2003-07-08

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