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HUP0203314A2 - Oleoyl-acyl-carrier-protein thioesterases in plants - Google Patents

Oleoyl-acyl-carrier-protein thioesterases in plants

Info

Publication number
HUP0203314A2
HUP0203314A2 HU0203314A HUP0203314A HUP0203314A2 HU P0203314 A2 HUP0203314 A2 HU P0203314A2 HU 0203314 A HU0203314 A HU 0203314A HU P0203314 A HUP0203314 A HU P0203314A HU P0203314 A2 HUP0203314 A2 HU P0203314A2
Authority
HU
Hungary
Prior art keywords
oleyl
acp
thioesterase
polynucleotide sequence
plants
Prior art date
Application number
HU0203314A
Other languages
Hungarian (hu)
Inventor
Stephen M. Allen
J. Antoni Rafalski
Jennie Bih-Jien Shen
Original Assignee
E.I. Du Pont De Nemours And Co.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I. Du Pont De Nemours And Co. filed Critical E.I. Du Pont De Nemours And Co.
Publication of HUP0203314A2 publication Critical patent/HUP0203314A2/en
Publication of HUP0203314A3 publication Critical patent/HUP0203314A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A találmány tárgyát képezi oleil-ACP- tioészterázt kódoló, izoláltpolinukleotid-szekvencia, a polinukleotidszekvenciát tartalmazó sejtés transzgénikus növény, és a polinukleotid-szekvenciát egy megfelelőszabályozó szekvenciához kapcsolva tartalmazó kiméra gén. A találmánytárgyát képezi továbbá eljárás oleil-ACP-tioészteráz expressziósszintjének megváltoztatására, amely során a találmány szerinti kiméragénnel transzformálnak egy gazdasejtet és a transzformált sejtet akiméra gén expressziója számára alkalmas körülmények közöttszaporítják. A találmány tárgyát képezi továbbá izolált oleil-ACP-tioész<- >teráz-polipeptid. A találmány a mezőgazdaság és azélelmiszeripar területén alkalmazható megváltozott magolaj-összetételű növények előállítására. ÓThe subject of the invention is an isolated polynucleotide sequence encoding oleyl-ACP thioesterase, a hypothetical transgenic plant containing the polynucleotide sequence, and a chimeric gene containing the polynucleotide sequence linked to a suitable regulatory sequence. The subject of the invention is also a method for changing the expression level of oleyl-ACP-thioesterase, during which a host cell is transformed with the chimera gene according to the invention and the transformed cell is propagated under conditions suitable for expression of the chimera gene. The invention also relates to an isolated oleyl-ACP-thioesterase polypeptide. The invention can be used in the field of agriculture and the food industry for the production of plants with a changed seed oil composition. HE

HU0203314A 1999-11-24 2000-11-22 Oleoyl-acyl-carrier-protein thioesterases in plants HUP0203314A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16751099P 1999-11-24 1999-11-24
PCT/US2000/032012 WO2001038502A2 (en) 1999-11-24 2000-11-22 Oleoyl-acyl-carrier-protein thioesterases in plants

Publications (2)

Publication Number Publication Date
HUP0203314A2 true HUP0203314A2 (en) 2003-01-28
HUP0203314A3 HUP0203314A3 (en) 2005-11-28

Family

ID=22607664

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0203314A HUP0203314A3 (en) 1999-11-24 2000-11-22 Oleoyl-acyl-carrier-protein thioesterases in plants

Country Status (8)

Country Link
EP (1) EP1232250A2 (en)
JP (1) JP2004508802A (en)
AU (1) AU1787101A (en)
BR (1) BR0015641A (en)
CA (1) CA2387044A1 (en)
HU (1) HUP0203314A3 (en)
MX (1) MXPA02005178A (en)
WO (1) WO2001038502A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG181114A1 (en) * 2009-12-18 2012-07-30 Cargill Inc Brassica plants yielding oils with a low total saturated fatty acid content
CA2837011C (en) 2010-05-25 2023-01-17 Cargill Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
US9695434B2 (en) 2010-05-25 2017-07-04 Cargill, Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
FR2966162B1 (en) * 2010-10-15 2014-08-29 Genoplante Valor OBTAINING PLANTS HAVING IMPROVED TOLERANCE TO A WATER DEFICIT

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512482A (en) * 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases
US6331664B1 (en) * 1997-05-05 2001-12-18 Dow Agrosciences Llc Acyl-ACP thioesterase nucleic acids from maize and methods of altering palmitic acid levels in transgenic plants therewith

Also Published As

Publication number Publication date
WO2001038502A3 (en) 2001-12-13
EP1232250A2 (en) 2002-08-21
CA2387044A1 (en) 2001-05-31
AU1787101A (en) 2001-06-04
WO2001038502A2 (en) 2001-05-31
HUP0203314A3 (en) 2005-11-28
BR0015641A (en) 2002-07-09
JP2004508802A (en) 2004-03-25
MXPA02005178A (en) 2003-01-28

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Legal Events

Date Code Title Description
FA9A Lapse of provisional patent protection due to relinquishment or protection considered relinquished