HUE025741T2 - Eljárás vízhasznosítás javítására növényekben - Google Patents
Eljárás vízhasznosítás javítására növényekben Download PDFInfo
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- HUE025741T2 HUE025741T2 HUE11155855A HUE11155855A HUE025741T2 HU E025741 T2 HUE025741 T2 HU E025741T2 HU E11155855 A HUE11155855 A HU E11155855A HU E11155855 A HUE11155855 A HU E11155855A HU E025741 T2 HUE025741 T2 HU E025741T2
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C—CHEMISTRY; METALLURGY
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8266—Abscission; Dehiscence; Senescence
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Claims (7)
- (Eljárás vízhasznosítás javítására növényekben)1. Eljárás vízhasznosítás javítására növényekben, amely eljárás magában foglalja: (a) növények populációjának transzformálását egy ízopentenil-transzferázt kódoló nukleinsav-szekvenciához működőképesen kapcsolt szeneszcencíaűndukáíhaio SARK promófert magában foglaló rekombináns expresszlós kazettával; és (b) egy Javított vízhasznosítással rendelkező növény szelektálását, ahol a SARK promoter (I) a SEQ ID Nö: 1 szekvenclájú prornóterhoz legalább 95% azonossággal rendelkezik, vagy (ií) a SEQ ID Nö: 1 5'-végének BOG bp-jához legalább 95% azonossággal rendelkezik.
- 2. Az 1. Igénypont szerinti eijárás, ahol a transzformáció lépését Agrobact&rium alkalmazásával hajtjuk végre.
- 3. Az 1. vagy 2. igénypont szerinti eijárás, ahol a SARK promoter Phaseotus vuigarís-ból származik.
- 4. Az előző Igénypontok bármelyike szerinti eljárás, ahol az Izopenteníl--transzferézt kódoló nukleinsav-szekveneía Agrobacíerknmből szármatlk.. 5. A 4. igénypont szerinti eljárás, ahol az izopentenil-transzferáz legalább 95% azonossággal rendelkezik a SEQ ID Nö: 3-hoz,
- 6. Az előző igénypontok bármelyike szerinti eljárás, ahol a növény egy kétszikű növény. •8. Az 1 - 8. igénypontok bármelyike szerinti eljárás, ahol a növény egy egyszikű növény. 9. A 8. igénypont szerinti eljárás, ahol a növény az Otyza nemzetségből származik,
- 10. Az 1 ~ 5, igénypontok bármelyike szerinti eljárás, ahol a növény az Asparagus, Atropa, Ävena, Brassica, Citrus, Otruflus. Capsicum, Cucumis, Cucurbita, Daucua, Fasiuca, Fiagaria, Glycine, Cossypium, Helianthus, Hail&rocaüis, Hordeum. Hycscamus, Lactuca, Linum, Loli um, Lycpersicon, Malus, Manihot, Majorana, Medicago, Niootiana, Oryza, Panieum, Pannesetum, Persea, Pisum, Pyrus, Prunus, Rapbanns, Smaie, Smecio, Sinapis, Solanum, Sorghum, Trigcmila, Jriticum, Wls, Vigna és Zea nemzetségek egyikéből származik.
- 11, Egy izopenteníl-transzferáz! kodolô nukfeínsav-szekvenciához működőképesen kapcsolt szeneszcenoia-indukáiható SARK promote?! magában foglaló rekombináns expressziós kazetta alkalmazása agy növény vízhasznosításának javítására, ahöl a SARK promoter (I) a SEQ ID NO: 1 szekvenciáin promóterhoz legalább 95% azonossággal rendelkezik, vagy (íi) a SEQ ID NO: 1 S’-végének SÖÖ bp-jához legalább 95% azonossággal rendelkezik, 12. A 11. igénypont szerinti alkalmazás, ahol a SARK prompter a 3. igénypontban meghatározott. 13. A 11, vagy 12, igénypont szerinti alkalmazás, ahol a nukleinsav-szek-vencia a 4. vagy 5. igénypontban meghatározott, 14. A 11 - 12, igénypontok bármelyike szerinti alkalmazás, ahol a növény a β -10. igénypontok bármelyikében megbatározott.
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| EP2121937A2 (en) * | 2006-12-08 | 2009-11-25 | The University of York | Regulation of plant metabolism |
| CN103108955B (zh) * | 2010-07-15 | 2015-11-25 | 工业研究与发展基金会有限公司 | 用于提高植物中非生物胁迫耐受性的核酸构建体 |
| US10126760B2 (en) | 2011-02-25 | 2018-11-13 | Mks Instruments, Inc. | System for and method of fast pulse gas delivery |
| US10031531B2 (en) | 2011-02-25 | 2018-07-24 | Mks Instruments, Inc. | System for and method of multiple channel fast pulse gas delivery |
| CN104884619B (zh) * | 2012-12-31 | 2017-09-08 | 创世纪种业有限公司 | 一种棉花异戊烯基转移酶ipt2及其编码基因与应用 |
| WO2017083757A1 (en) * | 2015-11-13 | 2017-05-18 | Board Of Regents Of The Nevada System Of Higher Education On Behalf Of The University Of Nevada, Reno | Methods of engineered tissue succulence in plants |
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| US5306862A (en) | 1990-10-12 | 1994-04-26 | Amoco Corporation | Method and composition for increasing sterol accumulation in higher plants |
| US5763243A (en) | 1994-12-08 | 1998-06-09 | Pioneer Hi-Bred International, Inc. | Reversible nuclear genetic system for male sterility in transgenic plants |
| US5689042A (en) | 1995-03-29 | 1997-11-18 | Wisconsin Alumni Research Foundation | Transgenic plants with altered senescence characteristics |
| AU1804799A (en) * | 1997-12-08 | 1999-06-28 | Vitality Biotechnologies, Inc. | Plant senescence-associated genes |
| US20050086718A1 (en) * | 1999-03-23 | 2005-04-21 | Mendel Biotechnology, Inc. | Plant transcriptional regulators of abiotic stress |
| WO2001085971A2 (en) * | 2000-05-10 | 2001-11-15 | Phycogen, Inc. | Transgenic plants incorporating_genes of zostera marina |
| AUPQ994600A0 (en) * | 2000-09-06 | 2000-09-28 | Agriculture Victoria Services Pty Ltd | Manipulation of plant senescene |
| BRPI0214830B1 (pt) | 2001-12-10 | 2015-10-27 | Thomas Schmülling | método para modificação de planta, morfologicamente, bioquimicamente e fisicamente |
| US7358419B2 (en) * | 2002-04-08 | 2008-04-15 | Pioneer Hi-Bred International, Inc. | Enhanced silk exsertion under stress |
| US7138566B2 (en) * | 2002-06-21 | 2006-11-21 | Regents Of The University Of California | Methods of modulating cytokinin related processes in a plant using B3 domain proteins |
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| BRPI0607732B1 (pt) | 2017-11-07 |
| US9624503B2 (en) | 2017-04-18 |
| CN101146439B (zh) | 2013-03-27 |
| BRPI0607732A2 (pt) | 2010-03-16 |
| PL2324703T3 (pl) | 2015-11-30 |
| EP1863334B1 (en) | 2011-11-02 |
| WO2006102559A3 (en) | 2007-02-22 |
| US20080282365A1 (en) | 2008-11-13 |
| EP2324703B1 (en) | 2015-05-06 |
| ES2376924T3 (es) | 2012-03-20 |
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