HU209532B - Cosmetic composition containing alga-suspension with high biological value and process for preparing the active ingredient - Google Patents

Abstract

Field of the Invention The present invention relates to a cosmetic composition, preferably a cream, lotion, or hair care composition comprising from 0.01 to 99.5% by weight of a solids suspension treated with a cellulose enzyme, as conventional additives such as solvents, fats, oils, emulsifiers. , preservatives, antioxidants, fragrances and other fillers. The invention also relates to a process for the preparation of an active ingredient. EN 209 532 B Description: 4 pages

Description

The present invention relates to a cosmetic composition containing a high biological value algae suspension, preferably as a cream, lotion or hair care, and to a process for preparing the active ingredient of the cosmetic composition.

Polysaccharides such as carrageenan or alginic acid and vitamins such as vitamin D are extracted from sea algae by physico-chemical processes and applied separately in cosmetic formulations (Bodorné, Cosmetic Materials, 1973, 129).

No. 2,209,856. German Patent Application Publication No. 4,198,195 discloses a composition for cosmetic wrap comprising, inter alia, plant extracts such as algae extract.

No. 155,615. According to a Czech patent, a water-alcoholic extract is prepared by chemical process from whole algae - so obviously a significant part of the algae does not pass into the extract - and from this it is used to make cosmetics, especially skin and hair care products.

No. 191,489. Hungarian patent specification discloses a cosmetic composition comprising live single-cell algae. The solution uses all of the algae, but does nothing to get the algae's intracellular population into the extracellular space in a well-utilized manner (in a state that is well absorbed by the skin).

In each of the first three literature, algae is extracted which is extracted by some method and a known active ingredient of whole algae is used in cosmetic formulations.

The fourth literature uses whole algae, but does nothing to make the "bioconcentrate" in algae directly applicable to the skin.

It has been found that a high biological value algae suspension obtained by enzymatic digestion of algae is advantageously used as an active ingredient in cosmetic formulations.

Accordingly, the present invention relates to a cosmetic composition, preferably a cream, lotion or hair care composition, containing from 0.001 to 99.5% by weight of a solids-treated algae suspension treated with a cellulose-degrading enzyme in conventional additives such as solvents, fats, oils, emulsifiers, preservatives, antioxidants. besides fragrances and other fillers.

The invention further relates to a process for the preparation of a high biological value algae suspension by sterile algae culture at a temperature of 10-40 ° C.

After digestion with cellulose degrading enzyme for 1 to 72 hours, the resulting algae suspension is separated.

Algae, except blue algae, are eukaryotic organisms that perform photosynthesis. Thus, they show great similarity with the flora. These include micro-sized algae (such as green algae) and macroscopic organisms (such as Laminaria species from marine algae). The green algae that

They are 2-50 pm in size and have all the features of a real plant. It is like a real plant with its roots, stems, leaves, flowers bloomed in a globe of 10 pm diameter. You can see the incredible biological intensity of a complete plant crammed into such a small place. Humanity has long been aware of this, with algae being used for nutrition even before our time. Sea algae have been or are primarily used for this purpose. Nowadays, it is becoming increasingly desirable to obtain such concentrated, highly biological algae foods in the form of press or tablets (Japan, USA, Western Europe) - (Jackson, D.F .: Algéé and Mán New York 1964). Of course, algae are also used for feeding and industrial purposes. For example, carrageenan, agar, alginic acid and the like are extracted from sea algae. They are mainly used by the food, pharmaceutical and cosmetics industries. However, the use of algae for this purpose completely destroys virtually all biologically valuable substances, such as vitamins, provitamins, unsaturated fatty acids, mucoproteins, lipoproteins and proteins (Round, F.E., The Biology of the Algae, London 1973).

Cautious methods such as ultrasound treatment well known in cell digestion do not destroy the intracellular contents of the algae, but very high molecular weight constituents such as hemicellulose forming the cell wall itself, but also pectins, mucoproteins, polysaccharides, starches .

It has been found that by enzymatic digestion, algae can be transformed into a cosmetic agent of natural origin that fully retains the active ingredients of the original algae. After opening the cell wall (which we do with enzymes), the cell body enters the extracellular space (CT) which is very important because very valuable active substances enclosed by the cell wall would not come in direct contact with the skin surface, so their absorption and effect would be greatly inhibited. Molecular weight, essentially non-absorbable polysaccharides, pectins, mucoproteins are degraded to low molecular weight "building blocks" to provide a cosmetically high bioavailability, similar to proteins (Parfümerie und Kosmetik, 66.2, 8585-96).

Any algae can be used as algae. Microalgae organisms such as green algae are preferred.

Preferred cellulose-degrading enzymes are mycelial homogenates of fungi of the Pleurotus fungi family, sterile homogenate extract of bovine rumen contents, and sterile lysates of Spirulaceae bacteria. Of course, only non-infectious members of the Spirulaceae bacterial family may be used.

Preferably, the process according to the present invention is carried out by sterile growth of algae by a method well known in the art (Dr. Lajos Felföldi, Algae Cultivation), followed by enzymatic reaction of the resulting algae suspension at 10-40 ° C. As a result, the cell wall of the algae is degraded, the cell contents are transferred to the extracellular space, where

Charids and mucoprotides also decompose into smaller "building blocks" by enzymes.

Thus, the algal cellulose (hemicellulose), pectin, polysaccharide, mucoprotein, starch content is transferred from the high degree of polymerization to the very favorable low molecular weight range by enzymes.

Particularly beneficial algae active ingredient can be prepared by using the enzyme complex extracted from oyster mushroom (Pleutorus ostreatus) according to the invention.

A sterile aqueous extract of oyster mushroom mycelium having a dry matter content of, for example, 1 g / l and then 10 to 40 ° C, preferably 37 ° C, is added to a sterile culture suspension of algae, e.g. The suspension is maintained for 1-72 hours. The enzymatic degradation results in the algae suspension turning into a slightly yellowish colloidal solution and no visible algae cell visible under microscopic examination. The resulting solution is directly suitable for the preparation of cosmetics

The cosmetic and personal care products according to the invention preferably contain from 0.01 to 90% by weight of the dry matter content of the algae suspension according to the invention, optionally the usual additives, preferably herbal extract, vegetable protein and known cosmetic additives for 100% by weight. , preferably carriers and other diluents such as animal or vegetable fats and oils and surfactants.

According to the invention, cosmetic agents containing an enzyme-digested algae suspension have an immediate effect and are immediately absorbed into the skin.

The compositions of the present invention greatly promote hydration of the dehydrated skin, regenerate the cells of the aging skin, and exert a beneficial effect on the skin of the nasopharynx.

All of this outstanding, very beneficial effect is due to the fact that it reacts with enzymes in algae

1.) the biologically active substances are released from the cell into the extracellular space without the least damage,

2.) high molecular weight polysaccharides, pectins, mucoproteins and the like are degraded to lower molecular weight parts with significantly better absorbability.

In other known digestion methods, such as chemical breakdown of the cell wall, heat treatment, mechanical treatments, ultrasound, essential fatty acids (arachidonic acid, EPA, DHA, gamma-linolenic acid) are in all cases significantly damaged. In the process of the invention, they are transferred from the intracellular nucleus to the extracellular extracellular space without the slightest damage, from where they can be immediately absorbed into the skin.

A particularly beneficial composition is obtained by admixing any known herbal extract or a plant protein. Preferably, additional carriers or other diluents, such as oils or fats of vegetable or animal origin, and surfactants are admixed with the composition.

The process and compositions of the present invention are illustrated by the following non-limiting examples.

Example 7

Preparation of algae suspension.

The algae suspension is prepared from algae cultures prepared according to methods known in the literature (Erzsébet Kol, László Machay, cultivated algae).

1 g of filtered tap water was added with 10 g of potassium nitrate, 2.0 g of dipotassium hydrogen phosphate, 1.0 g of magnesium sulfate and 20 ml of Arnon's trace element solution (Dr. Lajos Felföldi: Biological water classification, 219), followed by 2 N HCl to pH 6.5 and sterilized. The sterile medium is inoculated with a culture of Chlorella pyrenoidosa. Cultivation was continued for 20 days under artificial illumination with carbon dioxide gas (5 vol% CO ₂ gas mixed with air). The resulting algae suspension was pelleted and the supernatant was discarded. The algae concentrate thus obtained has a dry matter content of at least 5 g / l. This concentrate is reacted with the appropriate enzyme complexes. To the concentrate was added 4 g of sterile distilled aqueous homogenate of 4 g of mycelium (Pleurotus ostreatus) mycelium at 37 ° C and the suspension was allowed to stand for 24 hours. The resultant 2 liters of sterile colloidal solution is used as active ingredient.

Example 2

Preparation of algae suspension

In each case, the same procedure as in Example 1 was followed except that the strain Chlorella vulgaris was used.

Example 3

Preparation of algae suspension

In each case, the procedure of Example 1 was followed, except that the homogenate of the fungal mycelium of Pleurotus comucopiae was used as the enzyme complex.

Example 4

Preparation of algae suspension

In each case, the procedure of Example 1 was followed, except that a sterile bovine rumen homogenate was used as the enzyme complex.

Example 5

Preparation of algae suspension

In each case, the same procedure as in Example 1 was followed, except that a sterile lysate of Cellvibrio ochraceus (prepared by ultrasound) was used as the enzyme complex.

Example 6

Skin regenerating face and body care product

200 g of the algae suspension prepared in Example 1 are mixed with 200 g of distilled water and then mixed at 70 ° C with 65 g of white vaseline, 65 g of stea

Binine monolceride, 80 g of cetyl alcohol, 25 g of sunflower oil and 25 g of Tween 60 with a melting point of 70 ° C. After mixing, the emulsion is stirred until cooled and then 5 ml of 10% w / w nipagin-M preservative is added. The composition is excellent for skin regeneration and is also useful for seborrhea.

Example 7 Preparation for use in lontophoresis

The algae suspension prepared according to Example 2 was filled under sterile conditions into a 2 ml brown ampoule. The product is very active in cosmetic iontophoresis treatment.

Example 8

Preparation of moisturizing gel

8.0 g of Carbopol 940 were swelled in 700 ml of distilled water. To this was added 250 ml of the algae suspension prepared as in Example 3, and to the resulting solution was added 10.8 g of triethanolamine with constant stirring to obtain a gel consistency. The resulting gel can be used as an intensive facial mask for aging, dehydrated skin.

Example 9

Production of intensive bio-mask

100 g of polyvinyl alcohol are swelled in 600 ml of distilled water while warm. When the solution is homogeneous, it is cooled to room temperature and 200 ml of 96% ethanol are added. Then, 300 g of the algae suspension prepared in Example 4 is mixed with the solution thus prepared. The resulting mask evenly applies a very intense hydration to the face. After drying for 15-20 minutes, the mask can be peeled off. As a result, the skin is instantly visibly smoothed, soft, velvety. It is also excellent for treating seborrheic skin.

Example 10

Production of shampoo

To 500 g of the algae suspension of Example 1 is added 100 g of Texapon 25-25, followed by 6 ml of 10% w / w nipagin-M preservative. The product obtained is used as a hair care shampoo. Regular use reduces hair loss and dandruff, makes hair shiny and supple.

Example 11

Functional example

The effect of the bio-mask prepared according to Example 9 was tested in 25 subjects with seborrheic skin. Of the five injured persons, 20 were female and 5 were male. (The skin of the five men was slightly acne). Subjects used the preparation once daily for two weeks. The mask was lightly applied to lukewarm soapy water and then peeled off after about 1510 for 20 minutes.

The effect was apparent immediately after the first treatment.

The number of papules decreased significantly, the skin became smooth and pleasant to the touch. After two weeks of regular use, seborrhea complaints have virtually disappeared.

Claims (5)

PATENT CLAIMS Cosmetic composition, preferably cream, lotion or hair care, characterized in that it contains from 0.01 to 99.5% by weight of a solids-treated algae suspension treated with a cellulose-degrading enzyme, such as solvents, fats and other additives commonly used in the cosmetic industry. , oils, emulsifiers, preservatives, antioxidants, perfumes and other fillers. Composition according to claim 1, characterized in that it contains as a suspension of algae a suspension of microalgae organisms, preferably green algae. Composition according to claim 1 or 2, characterized in that it further contains an herbal extract or vegetable proteins, preferably soy protein. A process for preparing a high biological value algae suspension as an active ingredient of the composition of claim 1, wherein the sterile algae culture is digested with cellulose degrading enzyme at 10-40 ° C for 172 hours and the resulting algae suspension is isolated in a known manner. The method of claim 4, wherein the enzyme is selected from the group consisting of a mycelium of fungi of the Pleurotus fungi family, a sterile homogenate extract of bovine rumen content, or a sterile lysate of Spirulaceae.