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HU0401385D0 - Tissue cultivating method for culturing skin cells - Google Patents

Tissue cultivating method for culturing skin cells

Info

Publication number
HU0401385D0
HU0401385D0 HU0401385A HUP0401385A HU0401385D0 HU 0401385 D0 HU0401385 D0 HU 0401385D0 HU 0401385 A HU0401385 A HU 0401385A HU P0401385 A HUP0401385 A HU P0401385A HU 0401385 D0 HU0401385 D0 HU 0401385D0
Authority
HU
Hungary
Prior art keywords
skin cells
cultivating method
tissue cultivating
culturing skin
culturing
Prior art date
Application number
HU0401385A
Other languages
Hungarian (hu)
Original Assignee
Kemeny Lajos Dr
Csoergoene Dr Bata Zsuzsa Dr
Szabad Gabor Dr
Nagy Norbert Dr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kemeny Lajos Dr, Csoergoene Dr Bata Zsuzsa Dr, Szabad Gabor Dr, Nagy Norbert Dr filed Critical Kemeny Lajos Dr
Priority to HU0401385A priority Critical patent/HU227723B1/en
Publication of HU0401385D0 publication Critical patent/HU0401385D0/en
Priority to EP05763133A priority patent/EP1768716A1/en
Priority to PCT/HU2005/000076 priority patent/WO2006005977A1/en
Publication of HUP0401385A2 publication Critical patent/HUP0401385A2/en
Publication of HU227723B1 publication Critical patent/HU227723B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0626—Melanocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/091—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells melanocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dermatology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
HU0401385A 2004-07-09 2004-07-09 Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy HU227723B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
HU0401385A HU227723B1 (en) 2004-07-09 2004-07-09 Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy
EP05763133A EP1768716A1 (en) 2004-07-09 2005-07-11 Novel method for culturing keratinocytes, melanocytes and fibroblasts for skin grafting
PCT/HU2005/000076 WO2006005977A1 (en) 2004-07-09 2005-07-11 Novel method for culturing keratinocytes, melanocytes and fibroblasts for skin grafting

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU0401385A HU227723B1 (en) 2004-07-09 2004-07-09 Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy

Publications (3)

Publication Number Publication Date
HU0401385D0 true HU0401385D0 (en) 2004-09-28
HUP0401385A2 HUP0401385A2 (en) 2006-09-28
HU227723B1 HU227723B1 (en) 2012-01-30

Family

ID=89985359

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0401385A HU227723B1 (en) 2004-07-09 2004-07-09 Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy

Country Status (3)

Country Link
EP (1) EP1768716A1 (en)
HU (1) HU227723B1 (en)
WO (1) WO2006005977A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100303770A1 (en) * 2006-12-28 2010-12-02 John Maslowski Methods for culturing dermal cells for treatment of skin injuries such as burns
DE102007049402A1 (en) 2007-10-15 2009-04-16 Euroderm Gmbh Cosmetic method for increasing the pigmentation of skin using melanocyte progenitor cells
CA2700729A1 (en) * 2007-10-19 2009-04-23 The University Of Tokyo Therapeutic agent for vitiligo and method of accelerating pigmentation
GB2483622A (en) * 2009-08-03 2012-03-21 Univ Durham Fibroblast feeder cells
GB0916370D0 (en) * 2009-09-18 2009-10-28 Avecia Biolog Ltd Compositions
JP2013520163A (en) * 2010-02-23 2013-06-06 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル Method for preparing human melanocytes from human pluripotent stem cells
DE102010009571A1 (en) * 2010-02-26 2011-09-01 Euroderm Gmbh A method of promoting the restoration of bodily function using cells of the hair root sheath, preparation and manufacturing method
DE102010009572A1 (en) * 2010-02-26 2011-09-01 Euroderm Gmbh A method of promoting healing of skin wounds using the hair root sheath cells, preparation and method of manufacture
BR112012029546B8 (en) * 2010-05-21 2021-07-27 Rodrigo Focion Soto Pareja process for generating a patch or dressing with skin
JP6101066B2 (en) * 2012-12-10 2017-03-22 日本メナード化粧品株式会社 Preparation method of melanocyte population
CN107151648B (en) * 2017-06-18 2023-07-21 广东博溪生物科技有限公司 Culture medium for co-culture of melanocytes and keratinocytes
WO2019112327A2 (en) * 2017-12-06 2019-06-13 주식회사 이뮤노맥스 Method for producing cytotoxic t cells and use thereof
CN111647550A (en) * 2020-05-08 2020-09-11 嘉禾弘生(深圳)健康产业集团有限公司 Method for culturing human skin fibroblasts by tissue mass enzyme combination method
CN114522182A (en) * 2020-11-07 2022-05-24 海口仁术皮肤科门诊部有限公司 Preparation method of hair follicle melanin stem cell suspension for treating leucoderma
CN115227876B (en) * 2022-07-28 2023-08-29 福建省海西细胞生物工程有限公司 Preparation method of tissue engineering epidermis

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19958693C2 (en) * 1999-12-06 2002-07-18 Biotissue Technologies Gmbh Methods and compositions for making cell grafts

Also Published As

Publication number Publication date
EP1768716A1 (en) 2007-04-04
HUP0401385A2 (en) 2006-09-28
HU227723B1 (en) 2012-01-30
WO2006005977B1 (en) 2008-12-24
WO2006005977A1 (en) 2006-01-19

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Legal Events

Date Code Title Description
MM4A Lapse of definitive patent protection due to non-payment of fees