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GR1003966B - Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση η και προσδιορισμο ειδικων αλληλουχιων νουκλεικων οξεων - Google Patents

Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση η και προσδιορισμο ειδικων αλληλουχιων νουκλεικων οξεων

Info

Publication number
GR1003966B
GR1003966B GR20010100478A GR2001100478A GR1003966B GR 1003966 B GR1003966 B GR 1003966B GR 20010100478 A GR20010100478 A GR 20010100478A GR 2001100478 A GR2001100478 A GR 2001100478A GR 1003966 B GR1003966 B GR 1003966B
Authority
GR
Greece
Prior art keywords
nucleic acid
test strip
specific nucleic
acid sequences
determination
Prior art date
Application number
GR20010100478A
Other languages
English (en)
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Original Assignee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37616771&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=GR1003966(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by filed Critical
Publication of GR1003966B publication Critical patent/GR1003966B/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

μβαπτιζόμενος χάρτης αποξηραμένων αντιδραστηρίων, μέθοδος παραγωγής του, και μέθοδος για την ανίχνευση ή και προσδιορισμό ειδικών αλληλουχιών νουκλεϊκών οξέων με την ανάπτυξη προτοκόλλων δοκιμασιώνυβριδισμού σε αυτόν. Στο χάρτη αποξηραίνονται σφαιρίδια χρυσού συζευγμένα με ολιγονουκλεοτίδια, και στην περιοχή της μεμβράνης ακινητοποιείται η πρωτεϊνη στρεπταβιδίνη και ένα ολιγονουκλεοτίδιο. Ανιχνεύεται η παρουσία μιας ειδικής αλληλουχίας DNA όταν λαμβάνεται έγχρωμο σήμα ενδεικτικό της παρουσίας ή απουσίας του αναλύτη στην ζώνη ακινητοποίησης της στρεπταβιδίνης, και επιβεβαιώνεται η ολοκλήρωση της δοκιμασίας από την ανάπτυξη έγχρωμου σήματος ενδεικτικού της ολοκλήρωσης της δοκιμασίας στη ζώνη ακινητοποίησης του ολιγονουκλεοτιδίου. Ο εμβαπτιζόμενος χάρτης χρησιμεύει στην ταχεία ανίχνευση ήκαι προσδιορισμό της ειδικής αλληλουχίας νουκλεϊκών οξέων που δηλώνουν π.χ. την παρουσία ιϊκού ή βακτηριακού DNA ή DNA μεταλλαγμένου στο οποίο οφείλεται παθολογικός φαινότυπος ασθενή, στο υπό εξέτασηδείγμα μετά την εκλεκτική ενίσχυση της ειδικής αλληλουχίας στο δείγμα μέσω PCR. ΰ
GR20010100478A 2001-10-16 2001-10-16 Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση η και προσδιορισμο ειδικων αλληλουχιων νουκλεικων οξεων GR1003966B (el)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20010100478 2001-10-16

Publications (1)

Publication Number Publication Date
GR1003966B true GR1003966B (el) 2002-08-06

Family

ID=37616771

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20010100478A GR1003966B (el) 2001-10-16 2001-10-16 Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση η και προσδιορισμο ειδικων αλληλουχιων νουκλεικων οξεων

Country Status (15)

Country Link
US (1) US20050202427A1 (el)
EP (1) EP1436420B1 (el)
AT (1) ATE360708T1 (el)
AU (1) AU2002329496B2 (el)
BR (1) BR0206145A (el)
CA (1) CA2468802A1 (el)
CY (1) CY1106724T1 (el)
DE (1) DE60219813T2 (el)
DK (1) DK1436420T3 (el)
ES (1) ES2286276T3 (el)
GR (1) GR1003966B (el)
NO (1) NO20032697L (el)
PT (1) PT1436420E (el)
WO (1) WO2003033735A2 (el)
YU (1) YU48903A (el)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1601787A1 (en) 2002-11-07 2005-12-07 Automated Analysers and Diagnostic Reagents Medicon Hellas S.A. Dry reagent strip and nucleic acid detection

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DE602004032125D1 (de) * 2003-05-07 2011-05-19 Coris Bioconcept Sprl Einstufige oligochromatographische vorrichtung und verfahren zu ihrer verwendung
EP1657550A1 (en) * 2004-11-10 2006-05-17 Coris Bioconcept SPRL Double-sided device for multiplex dipstick immunodiagnostic
EP1840222B1 (en) * 2004-12-29 2011-02-23 Suzhou Yangtze Delta Academy of Bio-x Science Ltd. The optimum method of amplification on polymerase chain reaction
GB0503986D0 (en) * 2005-02-26 2005-04-06 Secr Defence Reaction apparatus
US8980561B1 (en) * 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
EP1933145B1 (en) * 2006-12-11 2011-09-28 AraGen Biotechnology Co. Ltd. Rapid immunochromatographic detection by amplification of the colloidal gold signal
WO2009005552A2 (en) * 2007-03-29 2009-01-08 Epitype Corporation Methods and compositions for multivalent binding and methods for manufacture of rapid diagnostic tests
GB0806041D0 (en) * 2008-04-03 2008-05-14 Genomica S A Method for detection of herpesvirus in test sample
CN102128832A (zh) * 2010-12-27 2011-07-20 江南大学 一种西瓜果斑病毒金标核酸试纸条及其制备和应用
CN102183633B (zh) * 2011-02-24 2013-05-15 南京基蛋生物科技有限公司 一种胶体金标记方法
US9651549B2 (en) * 2012-07-13 2017-05-16 Genisphere, Llc Lateral flow assays using DNA dendrimers
CN105188764B (zh) 2013-03-21 2020-03-20 詹尼斯费尔公司 Dna嵌入剂的细胞递送
FR3012982B1 (fr) * 2013-11-08 2015-12-25 Espci Innov Procede de stockage et de concentration d'un compose volatil
US9724523B2 (en) 2014-11-13 2017-08-08 Alfred E. Mann Foundation For Scientific Research EPG leaded interface
US9464969B2 (en) 2014-11-20 2016-10-11 Monolythix, Inc. Monoliths
US10808278B2 (en) 2015-03-03 2020-10-20 University Of Miami Kits and methods for pathogen detection
US10570439B2 (en) 2015-08-18 2020-02-25 Monolythix, Inc. Sample concentration devices
CN108977501A (zh) * 2018-08-13 2018-12-11 吉林农业科技学院 人参黑斑菌-单管巢式pcr-核酸传感器的制备及其检测方法
ES2932995A1 (es) * 2021-07-22 2023-01-30 Ocupharm Diagnostics Sl Sistema de detección de parásitos oculares

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WO2001000876A1 (en) * 1999-06-25 2001-01-04 Mirkin Chad A Nanoparticles having oligonucleotides attached thereto and uses therefor

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WO1992007096A1 (en) * 1990-10-19 1992-04-30 Microprobe Corporation Mehods and pharmaceutical kits useful for detecting microorganisms associated with vaginal infections
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
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US7101683B2 (en) * 2001-06-26 2006-09-05 Abbott Laboratories Methods for the simultaneous detection of HCV antigens and HCV antibodies

Patent Citations (2)

* Cited by examiner, † Cited by third party
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WO1990001564A1 (en) * 1988-08-09 1990-02-22 Microprobe Corporation Methods for multiple target analyses through nucleic acid hybridization
WO2001000876A1 (en) * 1999-06-25 2001-01-04 Mirkin Chad A Nanoparticles having oligonucleotides attached thereto and uses therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FONG WHALLEY K ET AL: "Rapid solid-phase immunoassay for detection of methicillin-resistant Staphylococcus aureus using cycling probe technology.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 7, July 2000 (2000-07-01), pages 2525 - 2529, XP002202273, ISSN: 0095-1137 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1601787A1 (en) 2002-11-07 2005-12-07 Automated Analysers and Diagnostic Reagents Medicon Hellas S.A. Dry reagent strip and nucleic acid detection

Also Published As

Publication number Publication date
CA2468802A1 (en) 2003-04-24
DE60219813D1 (de) 2007-06-06
EP1436420B1 (en) 2007-04-25
PT1436420E (pt) 2007-09-12
AU2002329496B2 (en) 2009-10-22
DE60219813T2 (de) 2008-01-31
ES2286276T3 (es) 2007-12-01
WO2003033735A3 (en) 2003-09-12
US20050202427A1 (en) 2005-09-15
YU48903A (sh) 2006-08-17
DK1436420T3 (da) 2007-09-10
BR0206145A (pt) 2004-01-13
NO20032697L (no) 2003-07-15
WO2003033735A2 (en) 2003-04-24
ATE360708T1 (de) 2007-05-15
EP1436420A2 (en) 2004-07-14
CY1106724T1 (el) 2012-05-23
NO20032697D0 (no) 2003-06-13

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