GB983064A - Analytical reagents and method of analysis - Google Patents
Analytical reagents and method of analysisInfo
- Publication number
- GB983064A GB983064A GB1060162A GB1060162A GB983064A GB 983064 A GB983064 A GB 983064A GB 1060162 A GB1060162 A GB 1060162A GB 1060162 A GB1060162 A GB 1060162A GB 983064 A GB983064 A GB 983064A
- Authority
- GB
- United Kingdom
- Prior art keywords
- alkali metal
- vial
- phenol
- nitroprusside
- urease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/62—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving urea
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A reagent combination for the colorimetric analysis of urea nitrogen in biological fluids comprises (1) a vial of an anhydrous mixture of phenol and an alkali metal nitroprusside; (2) a vial of an alkaline aqueous solution of alkali metal hypochlorite; and (3) a vial of buffered urease, there being a sufficient excess of alkali metal cations in said second vial to convert the whole of the phenol in the said first vial to an alkali metal phenate. The alkali metal hypochlorite may be NaClO and said excess alkali in the aqueous solution may be: NaOH, K2CO3, K3PO4 or KOH. The solid nitroprusside may be in the form of a single crystal or particulate and may be added to the melted phenol or the two mixed as solids under anhydrous conditions. To the mixture of buffered urease and urea the nitroprusside and phenol are added either sequentially or together, followed, after up to 2 hours, by the alkaline hypochlorite and the colour measured e.g. on a spectrophotometer. The urease may be buffered with: ethylene diamine tetraacetic acid, KH2PO4 or Na2HPO4.ALSO:A reagent for use in determining ammonia nitrogen in solution comprises an anhydrous mixture of phenol and an alkali metal nitroprusside, and may be part of a reagent combination for the colorimetric analysis of urea nitrogen in biological fluids comprising (1) a vial of an anhydrous mixture of phenol and an alkali metal nitroprusside, (2) a vial of an alkaline aqueous solution of alkali metal hypochlorite, and (3) a vial of buffered urease, there being a sufficient excess of alkali metal cations in said second vial to cnvert the whole of the phenol in the said first vial to an alkali metal phenate. The alkali metal hypochlorite may be NaClO and said excess alkali in the aqueous solution may be: NaOH, K2CO3, K3PO4 or KOH. The solid nitroprusside may be in the form of a single crystal or particulate and may be added to the melted phenol or the two mixed as solids under anhydrous conditions. To the mixture of buffered urease and urea the nitroprusside and phenol are added either sequentially or together, followed, after up to 2 hours, by the alkaline hypochlorite and the colour measured, e.g., on a spectrophotometer. The urease may be buffered with: ethylene diamine tetraacetic acid, KH2PO4 or Na2HPO4.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9672661A | 1961-03-20 | 1961-03-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB983064A true GB983064A (en) | 1965-02-10 |
Family
ID=22258795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1060162A Expired GB983064A (en) | 1961-03-20 | 1962-03-20 | Analytical reagents and method of analysis |
Country Status (5)
| Country | Link |
|---|---|
| BE (1) | BE615330A (en) |
| DE (1) | DE1295887C2 (en) |
| DK (1) | DK106250C (en) |
| GB (1) | GB983064A (en) |
| SE (1) | SE304390B (en) |
-
0
- BE BE615330D patent/BE615330A/xx unknown
-
1962
- 1962-03-19 DE DE19621295887 patent/DE1295887C2/en not_active Expired
- 1962-03-19 SE SE302962A patent/SE304390B/xx unknown
- 1962-03-20 GB GB1060162A patent/GB983064A/en not_active Expired
- 1962-03-20 DK DK127062A patent/DK106250C/en active
Also Published As
| Publication number | Publication date |
|---|---|
| BE615330A (en) | |
| SE304390B (en) | 1968-09-23 |
| DK106250C (en) | 1967-01-09 |
| DE1295887C2 (en) | 1974-05-02 |
| DE1295887B (en) | 1974-05-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chaney et al. | Modified reagents for determination of urea and ammonia | |
| JPS52117418A (en) | Kit for quantitative analysis and method for using the same | |
| Felker | Microdetermination of nitrogen in seed protein extracts with the salicylate-dichloroisocyanurate color reaction | |
| GB1361149A (en) | Reagent composition and method for determining calcium | |
| ATE52617T1 (en) | IMMUNOTEST PROCEDURE. | |
| ES2131543T3 (en) | MEASURING THE TIME OF ACTIVATED PARTIAL THROMBOPLASTINE (APTT) IN A SINGLE-STAGE REACTION. | |
| GB1213014A (en) | Testing blood for the presence of haemoglobins | |
| GB983064A (en) | Analytical reagents and method of analysis | |
| USRE26125E (en) | Reagents and analysis of nitrogen | |
| GB1236951A (en) | A process for the production of a solution of a molybdo-phosphate complex | |
| DE2937322C3 (en) | Method for the determination of urea | |
| JPS5322495A (en) | Automatic analyzer | |
| US3432395A (en) | Salicylate method for the quantitative determination of ammonia nitrogen | |
| GB1219624A (en) | Colour reagents for the determination of creatinine | |
| GB1294518A (en) | Test kit for use in the determination of uric acid in blood serum | |
| IE800221L (en) | Creatine kinase-mb determination. | |
| GB769289A (en) | Improvements in or relating to urine calcium test | |
| AU7137581A (en) | Chemical indicator reagent for pregnancy testing | |
| ES2026889T3 (en) | LIQUID SAMPLE DOSING PROCEDURE AND DEVICE. | |
| JPS57168144A (en) | Colorimetric measuring method | |
| JPS57206398A (en) | Dried culture medium for identifying cell | |
| JPS6469954A (en) | Immunological measuring method | |
| SU1006999A1 (en) | Ammonia determination method | |
| SU798590A1 (en) | Method of determining nitrogen content in biological materials | |
| JPS57184969A (en) | Measuring method for immunity of homo-pth |