GB822154A - Fermentation process for the production of d-arabitol - Google Patents

Abstract

Glycerol is prepared by fermenting a suitable nutrient medium under submerged and aerated conditions with a d-arabitol-producing strain of Saccharomyces rouxii or Saccaromyces mellis, the nutrient medium containing high-test cane molasses as the major source of assimilable carbon and also a further source of nitrogen such as urea, e.g. 0.05-0.1% w/v. It is preferred to add a further small proportion of an organic nitrogen source such as corn-steep liquor or yeast extract, e.g. 0.3-1.5% w/v. Invertase may also be added, e.g. about 0.02 to 0.3% w/v. Preferred total sugar concentration in the medium is about 15-25% w/v. Fermentation temperatures of about 30-37 DEG C. are suitable. The glycerine may be recovered by steam-distillation from the d-arabitol-containing ferment.ALSO:D-arabitol is prepared by fermenting under submerged and aerated conditions, a suitable nutrient medium with a d-arabitol-producing strain of Saccharomyces rouxii or Saccharomyces mellis wherein the nutrient medium contains high test cane molasses as the major source of assimilable carbon and also a further source of simple nitrogen such as urea, e.g. 0.05-0.1% w/v. It is preferred to add a further small proportion of an organic nitrogen source such as corn steep liquor or yeast extract, e.g. 0.3-1.5% w/v. Invertase may also be added, e.g. about 0.02 to 0.03% v/v. Preferred total sugar concentration in the medium is about 15-25% w/v. Fermentation temperatures of about 30-37 DEG C. are suitable. The d-arabitol may be recovered by extraction with a lower aliphatic alcohol after concentration.