GB765233A - Improvements in or relating to process for purification of relaxin by ion exchange - Google Patents
Improvements in or relating to process for purification of relaxin by ion exchangeInfo
- Publication number
- GB765233A GB765233A GB22604/54A GB2260454A GB765233A GB 765233 A GB765233 A GB 765233A GB 22604/54 A GB22604/54 A GB 22604/54A GB 2260454 A GB2260454 A GB 2260454A GB 765233 A GB765233 A GB 765233A
- Authority
- GB
- United Kingdom
- Prior art keywords
- relaxin
- resin
- purified
- buffered
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/2221—Relaxins
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F01—MACHINES OR ENGINES IN GENERAL; ENGINE PLANTS IN GENERAL; STEAM ENGINES
- F01K—STEAM ENGINE PLANTS; STEAM ACCUMULATORS; ENGINE PLANTS NOT OTHERWISE PROVIDED FOR; ENGINES USING SPECIAL WORKING FLUIDS OR CYCLES
- F01K9/00—Plants characterised by condensers arranged or modified to co-operate with the engines
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Pharmacology & Pharmacy (AREA)
- Endocrinology (AREA)
- Mechanical Engineering (AREA)
- Combustion & Propulsion (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
Abstract
Relaxin preparations containing impurities having an isoelectric point higher than that of relaxin are purified by bringing an aqueous solution having a pH greater than the isoelectric point of relaxin, say 7-8.6, into contact with a carboxylic acid type cation exchange resin buffered to the same pH, and washing unabsorbed material from the resin with a buffered solution of pH not greater than that of the buffered resin thereby recovering the purified relaxin. The resin may be regenerated by eluting the absorbed impurities with acid. Relaxin preparations containing impurities of lower isoelectric point are purified by bringing an aqueous solution of lower pH than the isoelectric point of relaxin, say 4.5-7, into contact with the said resin buffered to the same pH, and washing unabsorbed material from the resin, purified relaxin being then obtained by washing the resin with acid. Suitable resins are those formed by condensation of a phenolic carboxylic acid and an aldehyde, or preferably, copolymers of acrylic or methacrylic acid and divinyl benzene wherein the latter constitutes 2 1/2 -5 per cent of the resin composition. Prior to or after purification, electrolytes are removed by dialysis, or by treatment with a sulphonic acid type cation exchange resin (acid form), followed by treatment with an anion exchange resin. The product may be freeze-dried.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US765233XA | 1953-08-11 | 1953-08-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB765233A true GB765233A (en) | 1957-01-09 |
Family
ID=22132551
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB22604/54A Expired GB765233A (en) | 1953-08-11 | 1954-08-04 | Improvements in or relating to process for purification of relaxin by ion exchange |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB765233A (en) |
-
1954
- 1954-08-04 GB GB22604/54A patent/GB765233A/en not_active Expired
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