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GB2604692B - Method for preparing recombinant subunit vaccine against novel coronavirus - Google Patents

Method for preparing recombinant subunit vaccine against novel coronavirus Download PDF

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Publication number
GB2604692B
GB2604692B GB2115860.5A GB202115860A GB2604692B GB 2604692 B GB2604692 B GB 2604692B GB 202115860 A GB202115860 A GB 202115860A GB 2604692 B GB2604692 B GB 2604692B
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United Kingdom
Prior art keywords
vaccine against
subunit vaccine
novel coronavirus
preparing recombinant
recombinant subunit
Prior art date
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Active
Application number
GB2115860.5A
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GB2604692A (en
Inventor
Bi Shengli
Hou Yunde
Mao Jiangsen
Gao Fu
Wu Guizhen
Qin Chuan
Xu Wenbo
Mao Zi'an
Su Qiudong
Yi Yao
Gao Meng
Chen Gang
Zhuang Fangcheng
Lv Hangjun
Lu Shaohong
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Beijing Shangwei Biotechnology Dev Co Ltd
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Beijing Shangwei Biotechnology Dev Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • A61K39/215Coronaviridae, e.g. avian infectious bronchitis virus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/39Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/14Antivirals for RNA viruses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08RNA viruses
    • C07K14/165Coronaviridae, e.g. avian infectious bronchitis virus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/555Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55505Inorganic adjuvants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/55Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/20011Coronaviridae
    • C12N2770/20022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/20011Coronaviridae
    • C12N2770/20034Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/20011Coronaviridae
    • C12N2770/20051Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Epidemiology (AREA)
  • Communicable Diseases (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pulmonology (AREA)
  • Analytical Chemistry (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
GB2115860.5A 2020-11-04 2021-11-04 Method for preparing recombinant subunit vaccine against novel coronavirus Active GB2604692B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011216187.0A CN112089831B (en) 2020-11-04 2020-11-04 Preparation method of recombinant novel coronavirus subunit vaccine

Publications (2)

Publication Number Publication Date
GB2604692A GB2604692A (en) 2022-09-14
GB2604692B true GB2604692B (en) 2023-12-06

Family

ID=73784551

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2115860.5A Active GB2604692B (en) 2020-11-04 2021-11-04 Method for preparing recombinant subunit vaccine against novel coronavirus

Country Status (2)

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CN (1) CN112089831B (en)
GB (1) GB2604692B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113150171B (en) * 2021-04-25 2023-05-16 中国农业科学院兰州兽医研究所 African swine fever virus recombinant protein containing intramolecular adjuvant, expression vector and application
CA3219206A1 (en) * 2021-05-06 2022-11-10 Hipra Scientific, S.L.U. Sars-cov-2 subunit vaccine
CA3219201A1 (en) * 2021-05-06 2022-11-10 Hipra Scientific, S.L.U. Sars-cov-2 subunit vaccine
CN113801209B (en) * 2021-11-16 2022-02-11 浙江普康生物技术股份有限公司 A novel coronavirus recombinant protein with broad-spectrum neutralizing activity and preparation method thereof
CN114395015B (en) * 2021-12-17 2022-09-23 烟台派诺生物技术有限公司 Recombinant granular protein product suitable for industrial production and preparation method thereof
CN115073564A (en) * 2022-04-12 2022-09-20 北京尚位生物技术发展有限公司 Novel genetic engineering recombinant protein, recombinant vector, recombinant engineering bacterium and vaccine of coronavirus

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106146658A (en) * 2016-07-25 2016-11-23 黑龙江八农垦大学 A kind of preparation method of Recombinant Swine epidemic diarrhea virus antibody
CN106619543A (en) * 2016-12-15 2017-05-10 首都医科大学 Anti-tumor sea squirt polypeptide CS5931 freeze-dried powder needle preparation and preparation method thereof
WO2019008599A1 (en) * 2017-07-03 2019-01-10 Bharat Biotech International Limited A synthetic polypeptide epitope based vaccine composition
EP3818989A1 (en) * 2020-03-12 2021-05-12 Universität Greifswald Peptides and oligonucleotides for a sars-cov-2 vaccine
WO2021198999A1 (en) * 2020-04-03 2021-10-07 Axon Neuroscience Se Epitope-based vaccines for treatment of coronavirus associated diseases

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102153652A (en) * 2010-12-10 2011-08-17 浙江大学 Expression method and application of fusion protein
CN102633875A (en) * 2012-04-27 2012-08-15 天津生机集团股份有限公司 Renaturation liquid for recombining chicken alpha interferon inclusion body as well as preparation method and application thereof
CN111560074B (en) * 2020-03-20 2021-07-09 中山大学 A novel coronavirus S protein single-domain subunit nanovaccine based on Helicobacter pylori ferritin

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106146658A (en) * 2016-07-25 2016-11-23 黑龙江八农垦大学 A kind of preparation method of Recombinant Swine epidemic diarrhea virus antibody
CN106619543A (en) * 2016-12-15 2017-05-10 首都医科大学 Anti-tumor sea squirt polypeptide CS5931 freeze-dried powder needle preparation and preparation method thereof
WO2019008599A1 (en) * 2017-07-03 2019-01-10 Bharat Biotech International Limited A synthetic polypeptide epitope based vaccine composition
EP3818989A1 (en) * 2020-03-12 2021-05-12 Universität Greifswald Peptides and oligonucleotides for a sars-cov-2 vaccine
WO2021198999A1 (en) * 2020-04-03 2021-10-07 Axon Neuroscience Se Epitope-based vaccines for treatment of coronavirus associated diseases

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Vaccine, vol. 39, 2021, Qiu-Dong et al, " Recombinant SRS-CoV-2 RBD with a built in T helper epitope..." pages 1241-1247 *

Also Published As

Publication number Publication date
GB2604692A (en) 2022-09-14
CN112089831A (en) 2020-12-18
CN112089831B (en) 2021-04-02

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