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GB2485850C - Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplification - Google Patents

Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplification

Info

Publication number
GB2485850C
GB2485850C GB1100787.9A GB201100787A GB2485850C GB 2485850 C GB2485850 C GB 2485850C GB 201100787 A GB201100787 A GB 201100787A GB 2485850 C GB2485850 C GB 2485850C
Authority
GB
United Kingdom
Prior art keywords
partitioning
amplification
compositions
methods
copy number
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB1100787.9A
Other versions
GB2485850B (en
GB2485850A (en
GB201100787D0 (en
Inventor
Joseph Hindson Benjamin
Saxonov Serge
Belgrader Philip
Dean Ness Kevin
Lucero Michael
Wayne Colston Billy Jr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Priority claimed from PCT/US2010/058124 external-priority patent/WO2011066476A1/en
Publication of GB201100787D0 publication Critical patent/GB201100787D0/en
Publication of GB2485850A publication Critical patent/GB2485850A/en
Application granted granted Critical
Publication of GB2485850B publication Critical patent/GB2485850B/en
Publication of GB2485850C publication Critical patent/GB2485850C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB1100787.9A 2009-11-25 2010-11-25 Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplification Active GB2485850C (en)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US26459109P 2009-11-25 2009-11-25
US30984510P 2010-03-02 2010-03-02
US30983710P 2010-03-02 2010-03-02
US31763910P 2010-03-25 2010-03-25
US31768410P 2010-03-25 2010-03-25
US31763510P 2010-03-25 2010-03-25
US34106510P 2010-03-25 2010-03-25
US34121810P 2010-03-25 2010-03-25
US38098110P 2010-09-08 2010-09-08
US40910610P 2010-11-01 2010-11-01
US10947310P 2010-11-02 2010-11-02
US41076910P 2010-11-05 2010-11-05
PCT/US2010/058124 WO2011066476A1 (en) 2009-11-25 2010-11-25 Methods and compositions for detecting genetic material

Publications (4)

Publication Number Publication Date
GB201100787D0 GB201100787D0 (en) 2011-03-02
GB2485850A GB2485850A (en) 2012-05-30
GB2485850B GB2485850B (en) 2014-01-29
GB2485850C true GB2485850C (en) 2019-01-23

Family

ID=44066935

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1100787.9A Active GB2485850C (en) 2009-11-25 2010-11-25 Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplification

Country Status (3)

Country Link
CN (1) CN102985552B (en)
CA (1) CA2767028A1 (en)
GB (1) GB2485850C (en)

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US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN113528634A (en) 2012-08-14 2021-10-22 10X基因组学有限公司 Microcapsule compositions and methods
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3862435A1 (en) * 2013-02-08 2021-08-11 10X Genomics, Inc. Polynucleotide barcode generation
EP2986762B1 (en) * 2013-04-19 2019-11-06 Bio-Rad Laboratories, Inc. Digital analyte analysis
WO2015013681A1 (en) * 2013-07-25 2015-01-29 Bio-Rad Laboratories, Inc. Genetic assays
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
WO2015035555A1 (en) * 2013-09-10 2015-03-19 深圳华大基因科技有限公司 Method, system, and computer readable medium for determining whether fetus has abnormal number of sex chromosomes
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
KR20170026383A (en) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. Analysis of nucleic acid sequences
MX2016016902A (en) 2014-06-26 2017-03-27 10X Genomics Inc Methods of analyzing nucleic acids from individual cells or cell populations.
SG11201610691QA (en) 2014-06-26 2017-01-27 10X Genomics Inc Processes and systems for nucleic acid sequence assembly
CN104032031A (en) * 2014-07-04 2014-09-10 华东理工大学 PCR analyzing method for quantitatively detecting nucleic acid through RNA polymerase and ligase coupled reaction medium
MX2017005267A (en) 2014-10-29 2017-07-26 10X Genomics Inc Methods and compositions for targeted nucleic acid sequencing.
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
BR112017014902A2 (en) 2015-01-12 2018-03-13 10X Genomics Inc processes and systems for the preparation of nucleic acid sequencing libraries and libraries prepared using them
CN107209814B (en) 2015-01-13 2021-10-15 10X基因组学有限公司 System and method for visualizing structural variation and phase information
CN107208156B (en) 2015-02-09 2021-10-08 10X基因组学有限公司 System and method for determining structural variation and phasing using variation recognition data
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
MX2017010857A (en) 2015-02-24 2017-12-11 10X Genomics Inc Methods for targeted nucleic acid sequence coverage.
CN105936930A (en) * 2015-03-04 2016-09-14 松下知识产权经营株式会社 DNA detection method and DNA detection device
CN105969655A (en) * 2015-03-10 2016-09-28 松下知识产权经营株式会社 Method for analyzing multiple nucleic acid targets
CA2993347A1 (en) * 2015-07-29 2017-02-02 Progenity, Inc. Nucleic acids and methods for detecting chromosomal abnormalities
WO2017074094A1 (en) * 2015-10-29 2017-05-04 Biocore Co., Ltd. A method for prenatal diagnosis using digital pcr.
KR101848438B1 (en) 2015-10-29 2018-04-13 바이오코아 주식회사 A method for prenatal diagnosis using digital PCR
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
CN105821482B (en) * 2016-04-29 2018-04-10 李星军 A kind of biochemistry micro- reaction system, high-flux sequence build storehouse instrument and application
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN117512066A (en) 2017-01-30 2024-02-06 10X基因组学有限公司 Method and system for droplet-based single cell bar coding
CN110870018B (en) 2017-05-19 2024-11-22 10X基因组学有限公司 Systems and methods for analyzing data sets
EP4230746A3 (en) 2017-05-26 2023-11-01 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN107619827B (en) * 2017-09-12 2020-11-13 广州阳普医疗科技股份有限公司 Composition, preparation method thereof and application thereof in field of preservation of free DNA (deoxyribonucleic acid) in whole blood
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019204357A1 (en) * 2018-04-17 2019-10-24 ChromaCode, Inc. Methods and systems for multiplex analysis
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Also Published As

Publication number Publication date
CA2767028A1 (en) 2011-06-03
CN102985552A (en) 2013-03-20
GB2485850B (en) 2014-01-29
GB2485850A (en) 2012-05-30
GB201100787D0 (en) 2011-03-02
CN102985552B (en) 2016-02-17

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Legal Events

Date Code Title Description
COOA Change in applicant's name or ownership of the application

Owner name: BIO-RAD LABORATORIES, INC.

Free format text: FORMER OWNER: BIO-RAD QL, INC

S73 Revocation on comptroller's initiative (section 73/patents act 1977)

Free format text: SPECIFICATION AMENDED