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GB2466419B - RNA isolation method - Google Patents

RNA isolation method

Info

Publication number
GB2466419B
GB2466419B GB1006994A GB201006994A GB2466419B GB 2466419 B GB2466419 B GB 2466419B GB 1006994 A GB1006994 A GB 1006994A GB 201006994 A GB201006994 A GB 201006994A GB 2466419 B GB2466419 B GB 2466419B
Authority
GB
United Kingdom
Prior art keywords
rna isolation
isolation method
rna
isolation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB1006994A
Other versions
GB2466419A (en
GB201006994D0 (en
Inventor
Arne Deggerdal
Andrew Campbell
Veronica Boger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mole Genetics AS
Original Assignee
Mole Genetics AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mole Genetics AS filed Critical Mole Genetics AS
Publication of GB201006994D0 publication Critical patent/GB201006994D0/en
Publication of GB2466419A publication Critical patent/GB2466419A/en
Application granted granted Critical
Publication of GB2466419B publication Critical patent/GB2466419B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB1006994A 2007-09-28 2008-09-29 RNA isolation method Expired - Fee Related GB2466419B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0719022.6A GB0719022D0 (en) 2007-09-28 2007-09-28 Isolation method
PCT/EP2008/063039 WO2009040444A1 (en) 2007-09-28 2008-09-29 Rna isolation method

Publications (3)

Publication Number Publication Date
GB201006994D0 GB201006994D0 (en) 2010-06-09
GB2466419A GB2466419A (en) 2010-06-23
GB2466419B true GB2466419B (en) 2011-04-13

Family

ID=38701882

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB0719022.6A Ceased GB0719022D0 (en) 2007-09-28 2007-09-28 Isolation method
GB1006994A Expired - Fee Related GB2466419B (en) 2007-09-28 2008-09-29 RNA isolation method

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB0719022.6A Ceased GB0719022D0 (en) 2007-09-28 2007-09-28 Isolation method

Country Status (2)

Country Link
GB (2) GB0719022D0 (en)
WO (1) WO2009040444A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010088517A1 (en) * 2009-01-30 2010-08-05 The U.S.A., As Represented By The Secretary, Department Of Health And Human Services Methods and systems for purifying transferring and/or manipulating nucleic acids
FR2994184B1 (en) 2012-08-02 2017-12-08 Biomerieux Sa METHODS OF FUNCTIONALIZATION AND REAGENTS USED IN SUCH METHODS USING AN AZA-ISATOIC ANHYDRIDE OR ONE OF ITS DERIVATIVES, BIOLOGIC MOLECULES SO TREATED AND KITS
EP3135769A1 (en) * 2015-08-26 2017-03-01 Qiagen GmbH Kits and methods for extracting rna

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5681946A (en) * 1990-02-13 1997-10-28 Amersham International Plc Precipitating polymers
US5973137A (en) * 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
WO2003033739A1 (en) * 2001-10-12 2003-04-24 Gentra Systems, Inc. Compositions and methods for using a solid support to purify rna
WO2003046146A2 (en) * 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
WO2004108925A1 (en) * 2003-06-04 2004-12-16 Qiagen As Method for sequentially isolating dna and rna from the same nucleic acid-containing sample
EP1529841A1 (en) * 2003-11-07 2005-05-11 Hitachi High-Technologies Corporation RNA extraction method, RNA extraction reagent, and method for analyzing biological materials
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
WO2007051859A1 (en) * 2005-11-06 2007-05-10 Aj Innuscreen Gmbh Device and method for the automatic isolation and purification of nucleic acids from any complex starting materials
US20070190559A1 (en) * 1994-12-12 2007-08-16 Invitrogen Dynal As Isolation of nucleic acid

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5681946A (en) * 1990-02-13 1997-10-28 Amersham International Plc Precipitating polymers
US20070190559A1 (en) * 1994-12-12 2007-08-16 Invitrogen Dynal As Isolation of nucleic acid
US5973137A (en) * 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
WO2003033739A1 (en) * 2001-10-12 2003-04-24 Gentra Systems, Inc. Compositions and methods for using a solid support to purify rna
WO2003046146A2 (en) * 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
WO2004108925A1 (en) * 2003-06-04 2004-12-16 Qiagen As Method for sequentially isolating dna and rna from the same nucleic acid-containing sample
EP1529841A1 (en) * 2003-11-07 2005-05-11 Hitachi High-Technologies Corporation RNA extraction method, RNA extraction reagent, and method for analyzing biological materials
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
WO2007051859A1 (en) * 2005-11-06 2007-05-10 Aj Innuscreen Gmbh Device and method for the automatic isolation and purification of nucleic acids from any complex starting materials

Also Published As

Publication number Publication date
GB2466419A (en) 2010-06-23
WO2009040444A1 (en) 2009-04-02
GB201006994D0 (en) 2010-06-09
GB0719022D0 (en) 2007-11-07

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20120929