GB2422795A - Apparatus for processing biological sample - Google Patents
Apparatus for processing biological sample Download PDFInfo
- Publication number
- GB2422795A GB2422795A GB0602249A GB0602249A GB2422795A GB 2422795 A GB2422795 A GB 2422795A GB 0602249 A GB0602249 A GB 0602249A GB 0602249 A GB0602249 A GB 0602249A GB 2422795 A GB2422795 A GB 2422795A
- Authority
- GB
- United Kingdom
- Prior art keywords
- column
- permeable membrane
- protrusion
- receiving space
- liquid semi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012472 biological sample Substances 0.000 title claims abstract description 13
- 238000012545 processing Methods 0.000 title claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 109
- 239000012528 membrane Substances 0.000 claims abstract description 93
- 238000004891 communication Methods 0.000 claims description 12
- 239000000463 material Substances 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 239000000523 sample Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 7
- 238000012864 cross contamination Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
- B01L3/50255—Multi-well filtration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/20—Accessories; Auxiliary operations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
- C12M1/18—Multiple fields or compartments
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Water Supply & Treatment (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Peptides Or Proteins (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
An apparatus for processing biological sample which are commonly used to wash, separate and purify biochemical molecules, such as DNA, RNA and protein. The apparatus comprises at least one liquid semi-permeable membrane column 11, at least one vacuum connected column 12 a vacuum manifold 14 which are all loosely received to each other. An air tight material such as an air tight elastic band is disposed at a position where the liquid semi-permeable membrane column, the one vacuum connected column and the vacuum manifold engage with each other, thereby when the atmospheric pressure in the liquid semi-permeable membrane column is larger than that in the vacuum manifold, the apparatus is formed and maintained in an air tight state. If a liquid sample is placed inside liquid semipermeable membrane column, the atmospheric pressure can push the liquid out of the column through the liquid semi-permeable membranes. It is therefore much easier and convenient to assemble and disassemble the apparatus than a conventional one.
Description
Apparatus for Processing Biological Sample
Background of the Invention
1. Field of the Invention
The present invention relates to an apparatus for processing biological sample, especially the apparatus used to wash, separate and purif' biochemical molecules, such as DNA, RNA and protein.
2. Description of the Related Art
In biology laboratories, liquid semi-permeable membrane columns, which are generally cylindrical, are commonly used to wash, separate and purify biochemical molecules, such as DNA, RNA and protein. One or more pieces of specific purpose liquid semi-permeable membranes are placed at the bottom of the column and liquid is poured into the column. An adequate force is then applied to the liquid in the column, pushing the liquid out of the column through the liquid semi- permeable membranes.
Normally, the force applied is either a centrifugal force or atmospheric pressure.
When a centrifugal force is applied through the liquid semi-permeable membrane column, the column is usually placed into a liquid collecting tube, and the liquid is then poured into the liquid semi-permeable membrane column. The column and the liquid collecting tube are then placed into a centrifuge and spun at high speed generating a high centrifugal force which in turn, the liquid is pushed out of the column through the liquid semi-permeable membranes and is collected in the liquid collecting tube. However, it is a hassle for an operator to repeat the above procedure several times during continuous washing of the liquid semi-permeable membrane column. When atmospheric pressure is applied, several liquid semi- permeable membrane columns are inserted into a vacuum manifold. By applying * positive or negative atmospheric pressure, the liquid is pushed out of the columns *SS.
through the liquid semi-permeable membranes and is collected in the vacuum
S S S S. *
manifold. The atmospheric pressure procedure is more convenient to operate for multiple samples or continuous operations.
Closer inspection of the liquid semi-permeable membrane column (see Figure 1) shows that it is a geometric design consisting of three parts: an upper cervical section 311, a middle tubular section 3 12 and a lower tapered section 313. The diameter of the upper cervical section 311 is larger than that of the middle tubular section 312. Some of the columns contain a lid 314. The middle tubular section 312 is filled with liquid sample, and its interior bottom part contains one or more specific purpose liquid semi-permeable membranes (not shown in the figure).
Some of them have a design of lower tapered sections 313, but some of them do not.
As shown in Figure 1, the traditional engagement of the liquid semipermeable membrane column 31 with a vacuum manifold 32 is of a tight insertion style, i.e. the lower tapered section 313 of the liquid semipermeable membrane column 3 1 is directly inserted or through an insertable vacuum connected column 33 into a hole 34 of the vacuum manifold 32. The insertable vacuum connected column 33 is used to avoid the direct insertion of the liquid semi-permeable membrane column 31 into the hole 34 of the vacuum manifold 32, as the hole 34 of the vacuum manifold 32 may contact any liquid semi-permeable membrane column with different samples, which in turn, can lead to cross contamination of different samples. The insertable vacuum connected column 33 can be of a disposable type or simply cleaned for repeated use. When using the insertable vacuum connected column 33, the lower tapered section 3 13 of the liquid semi-permeable membrane column 31 is inserted into the insertable vacuum connected column 33. Then this: ensemble is inserted to the hole 34 of the vacuum manifold 32 which in turn, forms the following structure: the liquid semi-permeable membrane column 31 - the insertable vacuum connected column 33 - the vacuum manifold 32. Many applications utilise the insertable vacuum connected column 33, especially * ** * experiments which require no cross contamination of the samples, such as using. . . * I... * S * S. S
purified nuclear acid for PCR reaction. It is therefore very important that this engagement must be tightly secured to avoid any gas leak. Often, the operator has to hand-hold the liquid semi-permeable membrane column 31 and the insertable vacuum connected column 33 to ensure tight engagement. It is likely that the operator may experience discomfort of fingers due to this manoeuvre. On the other hand, the engagement sometimes can be difficult to disengage if it is too tight, and the multiple engagement sometimes can be difficult to operate, making it inconvenient to use the liquid semi-permeable membrane columns for multiple samples.
For the above-mentioned reasons, the applicant provides an apparatus comprising loosely engaged liquid semi-permeable membrane column, vacuum connected column and vacuum manifold for processing biological samples, whereas the operator can use this apparatus in a speedy and comfortable way.
Summary of the Invention
It is an object of the present invention to provide an apparatus for processing biological sample which is easier and convenient to assemble and disassemble.
The apparatus comprises at least one liquid semi-permeable membrane column, at least one vacuum connected colunm and a vacuum manifold, which are all loosely fit to each other. An air tight material, such as an air tight elastic band, is disposed at a position where the liquid semipermeable membrane column, the one vacuum connected column and the vacuum manifold engage with each other, thereby when the atmospheric pressure in the liquid semi-permeable membrane column is larger than that in the vacuum manifold, the apparatus is formed and maintained in an air S. tight state. If a liquid sample is placed inside liquid semi-permeable membrane column, the atmospheric pressure can push the liquid out of the column through liquid semi-permeable membranes. * I... * S... S * * *
The liquid semi-permeable membrane column comprises an inner section, a top section and a bottom section. The inner section defines a first receiving space with at least one liquid semi-permeable membrane placed at the bottom thereof. The top section is formed with a first aperture and has a first protrusion extending outwards from the top section. The bottom section is formed with a first through hole.
The vacuum connected column comprises an inner section, a top section and a bottom section. The inner section defines a second receiving space. The top section is formed with a second aperture and has a second protrusion extending outwards from the top section. The bottom section is formed with a second through hole. The vacuum manifold comprises a base defining a receiving space and a lid having at least one slot extending downwards therefrom for receiving at least one vacuum connected column. The slot is formed with a third through hole in communication with the receiving space of the base.
The liquid semi-permeable membrane column is loosely received in the second receiving space of the vacuum connected column such that the first protrusion of the liquid semi-permeable membrane column abuts against the second protrusion of the vacuum connected column. An air tight element, such as an air tight elastic band, is disposed around the periphery of the second protrusion. The vacuum connected column is loosely received in the slot such that the second protrusion abuts against an upper surface of the lid around the edge of the slot, and the first, the second and the third through holes are in communication, thereby when the atmospheric pressure in the first receiving space of the liquid semi-permeable membrane column is larger than that in the slot of the vacuum manifold, an air: ..
tight state is maintained between the first protrusion of the liquid semipermeable membrane column and the second protrusion of the vacuum connected column, and between the second protrusion of the vacuum connected column and the upper * surface of the lid around the edge of the slot.
* .. . * S **0* *... * * * ** S
In the other embodiment according to the present invention, an air tight element is sandwiched between the bottom section of the vacuum connected column and a bottom of the slot such that when the atmospheric pressure in the first receiving space of the liquid semi-permeable membrane column is larger than that in the slot of the vacuum manifold, an air tight state is formed and maintained between the first protrusion of the liquid semi-permeable membrane column and the second protrusion of the vacuum connected column, and between the bottom section of the vacuum connected column and the bottom of the slot. In addition, the liquid semi-permeable membrane column can also engage the vacuum manifold directly and an air tight element can be disposed around the periphery of the first protrusion or sandwiched between the bottom section of the liquid semi- permeable membrane column and the bottom of the slot to achieve the same air tight effect.
Other objects, advantages and novel features of the present invention will be drawn from the following detailed description of preferred embodiments of the present invention with the accompanying drawings, in which:
Descriptions of the Drawin2s
Fig. 1 illustrates a schematic view of a conventional apparatus for processing biological sample; Fig. 2 illustrates a schematic view of a conventional liquid semi-permeable membrane column used in conjunction with a vacuum connected column according to the present invention; Fig. 3 illustrates a perspective view of an apparatus for processing biological sample according to the present invention; S...
Fig. 4 illustrates a cross-sectional view of Fig. 3; Fig. 5 illustrates a perspective view of an alternative embodiment of the apparatus according to the present invention; Fig. 6 illustrates a cross-sectional view of Fig. 5; * *. S * S *..* S S *
S
Fig. 7 illustrates a perspective view of an alternative embodiment of the apparatus according to the present invention; Fig. 8 illustrates a crosssectional view of Fig. 7; Fig. 9 illustrates a perspective view of an alternative embodiment of the apparatus according to the present invention; and Fig. 10 illustrates a cross-sectional view of Fig. 9.
Detailed Description of the Invention
Fig. 2 illustrates a schematic view of a conventional liquid semipermeable membrane column used in conjunction with a vacuum connected column according to the present invention. Generally the liquid semipermeable membrane column 11 is of a tubular shape and comprises an inner section, a top section and a bottom section. The inner section defines a first receiving space 111 and at least one liquid semi-permeable membrane 112 is placed at the bottom of the first receiving space 111. The top section is formed with a first aperture (not shown in figure) and a first protrusion 113 extends outwards from the top section while the bottom section is formed with a first through hole 114.
Generally the vacuum connected column 12 is of a tubular shape and can be made of any suitable material. The vacuum connected column 12 comprises an inner section, a top section and a bottom section. The inner section defines a second receiving space 121. The top section is formed with a second aperture (not shown in figure) and a second protrusion 122 extends outwards from the top section while the bottom section is formed with a second through hole 123. The inner diameter of the vacuum connected column 12 is larger than that of the middle tubular S...
section of the liquid semi-permeable membrane column 11 but is smaller than that of the first protrusion 113 of the liquid semi-permeable membrane column 11 such that when the liquid semi-permeable membrane column 11 is loosely received in * .:* the second receiving space 121 of the vacuum connected column 12 through the second aperture, the first protrusion 113 of the liquid semi-permeable membrane column 11 is adapted to abut against the second protrusion 122 of the vacuum connected column 12. Further, an air tight element, preferably an air tight elastic band 13, can be disposed to surround the periphery of the second protrusion 122 of the vacuum connected column 12 to enhance air tightness between the first protrusion 113 and the second protrusion 122.
Referring to Figs. 3 and 4, which illustrate a perspective view of an apparatus 10 for processing biological sample according to the present invention and a cross- sectional view thereof. The apparatus 10 comprises at least one liquid semi- permeable membrane column 11, at least one vacuum connected column 12 and a vacuum manifold 14. The vacuum manifold 14 comprises a lid 141 and a base 142. The base 142 defines a receiving space 145 (referring to Fig. 4). The lid 141 has an upper surface and at least one slot 143 extending downwards therefrom for receiving at least one vacuum connected column 12. The slot 143 has a bottom which is formed with a third through hole 144 which in turn, is in communication with the receiving space 145 of the base 142.
The diameter of the slot 143 is slightly larger than that of the middle tubular section of the vacuum connected column 12 but is smaller than that of the second protrusion 122 of the vacuum connected column 12 such that the vacuum connected column 12 can be loosely received in the slot 143 when the liquid semi- permeable membrane column 11 is received in the vacuum connected column 12.
The second protrusion 122 is then adapted to abut against the upper surface of the lid 141 around the edge of the slot 143 and the first, the second and the third through holes 114, 123, 144 are in communication. In addition, any slot 143 which does not receive the vacuum connected column 12 should be air tightened: .. S...
by other suitable means, such as by inserting a conventional collection tube into...
the slot 143. An air tight element, preferably an air tight elastic band 13, can be disposed to surround the periphery of the second protrusion 122 of vacuum connected column 12. Thereby when the atmospheric pressure in the first receiving space 111 of the liquid semi-permeable membrane column 11 is larger
S S S
than that in the slot 143 of the vacuum manifold 14, an air tight state is formed and maintained between the first protrusion 113 of the liquid semi-permeable membrane column 11 and the second protrusion 122 of the vacuum connected column 12, and between the second protrusion 122 of the vacuum connected colunm 12 and the upper surface of the lid 141 around the edge of the slot 143. At the same time the atmospheric pressure is then applied to liquid in the liquid semi- permeable membrane column 11 and pushes the liquid out of the liquid semi- permeable membrane column 11 through the liquid semi-permeable membranes 112 (referring to Fig. 4). The purpose of the vacuum connected columns 12 can not only provide loosely fitting and air tightness effects but also prevents each slot 143 of the vacuum manifold 14 from contacting the different liquid semi- permeable membrane columns 11 with different samples when in repeated operations, which can lead to cross contamination.
Similarly, when a vacuum connected column 12' is loosely and partly received in a slot 143', the air tight engagement between liquid semipermeable membrane column 11', the vacuum connected column 12' and vacuum manifold 14' is illustrated in Figs. 5 and 6. An air tight elastic band 125' is sandwiched between the bottom section of the vacuum connected column 12' and a bottom of the slot 143'. Thereby when the atmospheric pressure in a first receiving space 111' of the liquid semi-permeable membrane column 11' is larger than that in the slot 143' of the vacuum manifold 14', an air tight state is maintained between a first protrusion 113' of the liquid semi-permeable membrane column 11' and a second protrusion 122' of the vacuum connected column 12', and between the bottom section of the vacuum connected column 12', the air tight elastic band 125' and the bottom of the slot 143'. An air tight element, preferably an air tight elastic band 13', can be further disposed to surround the periphery of the second protrusion 122' of the vacuum connected column 12' to enhance air tightness between the first protrusion 113' and the second protrusion 122'. * S *... S.,. I * I S. S
Referring to Figs. 7 to 10, liquid semi-permeable membrane columns 11", 11" can directly engage vacuum manifolds 14", 14", respectively. Air tight elastic bands 13", 125" can be disposed to surround the periphery of a first protrusion 113" of the liquid semi-permeable membrane columns 11", or be sandwiched between the bottom section of the liquid semi-permeable membrane column 11" around the periphery of a first through hole 1 14" and a bottom of slot 143"', respectively.
Thereby when the atmospheric pressure in a first receiving space 111" of the liquid semi-permeable membrane column 11" is larger than that in the slot 143" of the vacuum manifold 14", an air tight state is maintained between a first protrusion 113" of the liquid semi-permeable membrane column 11" and an upper surface of a lid 141" around the edge of the slot 143", or when the atmospheric pressure in a first receiving space 111" of liquid semi- permeable membrane column 11" is larger than that in a receiving space 145" of the base 142", an air tight state is maintained between the bottom section of the liquid semi-permeable membrane column 11" and the bottom of the slot 143".
From the above descriptions, it is apparent that the present invention provides an apparatus which is easy to assemble and disassemble, and can overcome the defects in the prior art. Since the liquid semi-permeable membrane column can be easily inserted and pulled out, it can also be operated in an automated process by a mechanical arm. While the invention has been described in terms of several preferred embodiments, those skilled in the art will recognise that the invention can still be practiced with modifications, within the spirit and scope of the appended claims. * S * * ** * 0 *SS I. * * 0 0 * S. * .** * * **. **e. S * S 9A
Attention is directed to all papers and documents which are filed concurrently with or previous to this specification in connection with this application and which are open to public inspection with this specification, and the contents of all such papers and documents are incorporated herein by reference.
All of the features disclosed in this specification (including any accompanying claims, abstract and drawings), and/or all of the steps of any method or process so disclosed, may be combined in any combination, except combinations where at least some of such features and/or steps are mutually exclusive.
Each feature disclosed in this specification
(including any accompanying claims, abstract and drawings) may be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise. Thus, unless expressly stated otherwise, each feature disclosed is one example only of a generic series of equivalent or similar features.
The invention is not restricted to the details of the foregoing embodiment(s). The invention extends to any novel one, or any novel combination, of the features
disclosed in this specification (including any
accompanying claims, abstract and drawings), or to any novel one, or any novel combination, of the steps of any method or process so disclosed. p * * ..* * a *a*. -S. S * S. P
Claims (10)
- What is claimed is: 1. An apparatus for processing biological sample,comprising: at least one liquid semi-permeable membrane column, comprising: an inner section defining a first receiving space, the first receiving space having at least one liquid semi-permeable membrane at the bottom thereof; a top section formed with a first aperture and a first protrusion extending outwards from the top section; and a bottom section formed with a first through hole; at least one vacuum connected column, comprising: an inner section defining a second receiving space; a top section formed with a second aperture and a second protrusion extending outwards from the top section; and a bottom section formed with a second through hole; and a vacuum manifold, comprising: a base defining a receiving space; and a lid having at least one slot extending downwards therefrom for receiving at least one vacuum connected column, the at least one slot formed with a third through hole in communication with the receiving space of the base; characterised in that: the liquid semi-permeable membrane column is loosely received in the second receiving space of the vacuum connected column such that the first protrusion of liquid semi-permeable membrane column abuts against the second protrusion of the vacuum connected column; an air tight element is disposed around the periphery of the second protrusion; the vacuum connected column is loosely received in the * slot such that the second protrusion abuts against an upper surface of the lid around the edge of the slot, and the first, the second and the, third through holes are in communication; thereby when the atmospheric pressure in the first receiving space of the liquid semi- -I. S * S.permeable membrane column is larger than that in the slot of the vacuum manifold, an air tight state is formed and maintained between the first protrusion of the liquid semi-permeable membrane column and the second protrusion of the vacuum connected column, and between the second protrusion of the vacuum connected column and the upper surface of the lid around the edge of the slot.
- 2. The apparatus according to Claim 1, wherein the air tight element is an air tight elastic band.
- 3. A vacuum connected column for the apparatus according to Claim 1, comprising: an inner section defining a receiving space; a top section formed with an aperture and a protrusion extending outwards from the top section; an air tight element disposed around the periphery of the protrusion; and a bottom section formed with a through hole.
- 4. An apparatus for processing biological sample, comprising: at least one liquid semi-permeable membrane column, comprising: an inner section defining a first receiving space, the first receiving space having at least one liquid semi-permeable membrane at the bottom thereof; a top section formed with a first aperture and a first protrusion extending outwards from the top section; and a bottom section formed with a first through hole; at least one vacuum connected column, comprising: an inner section defining a second receiving space; : .. S...a top section formed with a second aperture and a second protrusion S extending outwards from the top section; and a bottom section formed with a second through hole; and *.a vacuum manifold, comprising: S... * S S... * S.. * S * *.a base defining a receiving space; and a lid having at least one slot extending downwards therefrom for receiving at least one vacuum connected column, the at least one slot formed with a third through hole in communication with the receiving space of the base; characterised in that: the liquid semi-permeable membrane column is loosely received in the second receiving space of the vacuum connected column such that the first protrusion of the liquid semi-permeable membrane column abuts against the second protrusion of the vacuum connected column; an air tight element is disposed around the periphery of the second protrusion; the vacuum connected column is loosely and partly received in the slot such that the first, the second and the third through holes are in communication; an air tight element is sandwiched between the bottom section of the vacuum connected column and a bottom of the slot; thereby when the atmospheric pressure in the first receiving space of the liquid semi-permeable membrane column is larger than that in the slot of the vacuum manifold, an air tight state is formed and maintained between the first protrusion of the liquid semi- permeable membrane column and the second protrusion of the vacuum connected column, and between the bottom section of the vacuum connected column and the bottom of the slot.
- 5. The apparatus to Claim 4, wherein the air tight element is an air tight elastic band.
- 6. A vacuum connected column for the apparatus according to Claim 4, **. :* comprising: I. an inner section defining a receiving space; a top section formed with an aperture and a protrusion extending outwards from the top section; an air tight element disposed around the periphery of the protrusion; and. ...* S... S...S S SSS Sa bottom section formed with a through hole.
- 7. An apparatus for processing biological sample, comprising: at least one liquid semi-permeable membrane column, comprising: an inner section defining a first receiving space, the first receiving space having at least one liquid semi-permeable membrane at the bottom thereof; a top section formed with a first aperture and a first protrusion extending outwards from the top section; and a bottom section formed with a first through hole; and a vacuum manifold, comprising: a base defining a receiving space; and a lid having at least one slot extending downwards therefrom for receiving at least one liquid semi-permeable membrane column, the at least one slot formed with a second through hole in communication with the receiving space of the base; characterised in that: the liquid semi-permeable membrane column is loosely received in the slot such that the first protrusion of the liquid semi-permeable membrane column abuts against an upper surface of the lid around the edge of the slot, and the first and the second through holes are in communication; an air tight element is disposed around the periphery of the first protrusion; thereby when the atmospheric pressure in the first receiving space of the liquid semi-permeable membrane column is larger than that in the slot of the vacuum manifold, an air tight state is formed and maintained between the first protrusion of the liquid semi- permeable membrane column and the upper surface of the lid around the edge of the slot.
- 8. band. to Claim 7, wherein the air tight element is an air tight elastic * ** * * S SS*S S... S S * S*
- 9. An apparatus for processing biological sample, comprising: at least one liquid semi-permeable membrane column, comprising: an inner section defining a first receiving space, the first receiving space having at least one liquid semi-permeable membrane at the bottom thereof; a top section formed with a first aperture and a first protrusion extending outwards from the top section; and a bottom section formed with a first through hole; and a vacuum manifold, comprising: a base defining a receiving space; and a lid having at least one slot extending downwards therefrom for receiving at least one liquid semi-permeable membrane column, the at least one slot formed with a second through hole in communication with the receiving space of the base; characterised in that: the liquid semi-permeable membrane column is loosely and partly received in the slot; an air tight element is sandwiched between the bottom section of the liquid semi-permeable membrane column and a bottom of the slot such that the first and the second through holes are in communication; thereby when the atmospheric pressure in the first receiving space of the liquid semi-permeable membrane column is larger than that in the receiving space of the base, an air tight state is formed and maintained between the bottom section of the liquid semi- permeable membrane column and the bottom of the slot.
- 10. The apparatus to Claim 9, wherein the air tight element is an air tight elastic band. * S S. S. * * S S * *. * SS * S S...* * S5 S * S *S S
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW094103773A TWI253957B (en) | 2005-02-04 | 2005-02-04 | Apparatus for processing biochemical sample |
Publications (3)
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| GB0602249D0 GB0602249D0 (en) | 2006-03-15 |
| GB2422795A true GB2422795A (en) | 2006-08-09 |
| GB2422795B GB2422795B (en) | 2010-12-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0602249A Expired - Fee Related GB2422795B (en) | 2005-02-04 | 2006-02-06 | Apparatus for processing biological sample |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JP4459172B2 (en) |
| KR (1) | KR100830283B1 (en) |
| DE (1) | DE102006003777B4 (en) |
| FR (1) | FR2882943B1 (en) |
| GB (1) | GB2422795B (en) |
| TW (1) | TWI253957B (en) |
Cited By (5)
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| CN101440363B (en) * | 2007-11-23 | 2012-05-02 | 诺贝尔生物有限公司 | Method used to wash the column |
| WO2012120169A1 (en) | 2011-03-09 | 2012-09-13 | Zf Biotox, S.L. | Microplate for biological tests |
| US20130264286A1 (en) * | 2012-04-06 | 2013-10-10 | Cells Scientific Corporation | Biological sample filtering system and method for filtering biological samples |
| WO2019067195A1 (en) * | 2017-09-29 | 2019-04-04 | Siemens Healthcare Diagnostics Inc. | Test tube vacuum retainer |
| US10464060B2 (en) * | 2011-11-10 | 2019-11-05 | BioFare Diagnostics, LLC | Loading vials |
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| TW200907066A (en) * | 2007-08-13 | 2009-02-16 | Taigen Bioscience Corp | Method for washing a column and method for extracting membrane-bound target molecules |
| KR101080607B1 (en) * | 2009-03-27 | 2011-11-08 | 주식회사 인트론바이오테크놀로지 | Biological Sample Extractor |
| KR20090129387A (en) * | 2009-11-05 | 2009-12-16 | 주식회사 인트론바이오테크놀로지 | Apparatus for Extracting Biochemicals from Biological Samples |
| TWI406712B (en) * | 2011-05-18 | 2013-09-01 | Taigen Bioscience Corp | Apparatus for securely processing biological sample |
| KR101333016B1 (en) | 2011-11-22 | 2013-11-27 | 한국원자력의학원 | Jig for Blood irradiation Apparatus |
| EP3845884A4 (en) * | 2018-08-31 | 2022-06-01 | HORIBA Advanced Techno, Co., Ltd. | Sample preprocessor and analysis system |
| CN112517102A (en) * | 2020-06-17 | 2021-03-19 | 中国科学技术大学 | Many specifications are heat preservation centrifuging tube support for cell culture |
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Also Published As
| Publication number | Publication date |
|---|---|
| TW200628228A (en) | 2006-08-16 |
| KR20060089668A (en) | 2006-08-09 |
| DE102006003777A1 (en) | 2007-01-18 |
| GB0602249D0 (en) | 2006-03-15 |
| FR2882943A1 (en) | 2006-09-15 |
| TWI253957B (en) | 2006-05-01 |
| JP4459172B2 (en) | 2010-04-28 |
| DE102006003777B4 (en) | 2010-06-10 |
| GB2422795B (en) | 2010-12-08 |
| JP2006212028A (en) | 2006-08-17 |
| FR2882943B1 (en) | 2009-10-30 |
| KR100830283B1 (en) | 2008-05-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 20150206 |