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GB2375112A - Combinatorial protein domains - Google Patents

Combinatorial protein domains Download PDF

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Publication number
GB2375112A
GB2375112A GB0217404A GB0217404A GB2375112A GB 2375112 A GB2375112 A GB 2375112A GB 0217404 A GB0217404 A GB 0217404A GB 0217404 A GB0217404 A GB 0217404A GB 2375112 A GB2375112 A GB 2375112A
Authority
GB
United Kingdom
Prior art keywords
protein
chimaeric
designed
consist solely
sequence segments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0217404A
Other versions
GB0217404D0 (en
Inventor
Lutz Riechmann
Greg Winter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Domantis Ltd
Original Assignee
Domantis Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0002492A external-priority patent/GB0002492D0/en
Priority claimed from GB0016346A external-priority patent/GB0016346D0/en
Priority claimed from GB0019362A external-priority patent/GB0019362D0/en
Application filed by Domantis Ltd filed Critical Domantis Ltd
Publication of GB0217404D0 publication Critical patent/GB0217404D0/en
Publication of GB2375112A publication Critical patent/GB2375112A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The present invention relates to a chimaeric folded protein domain comprising two or more sequence segments from parent amino acid sequences that are not homologous, wherein each of said sequence segments: is not designed or selected to consist solely of a single complete protein structural element and is not designed or selected to consist solely of an entire protein domain; and, in isolation, shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains; and to a chimaeric folded protein domain comprising two or more sequence segments which share common sequences or sequences from common regions in the protein fold of their parent amino acid sequences, wherein the said common region of the fold is not designed or selected to consist solely of one or more complete protein structural elements, and wherein each of said sequence segments: is not designed or selected to consist solely of an entire protein domain; and wherein, in isolation, each said sequence segment shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains.

Description

(57) The present invention relates to a chimaeric folded protein domain
comprising two or more sequence segments from parent amino acid sequences that are not homologous, wherein each of said sequence segments: is not designed or selected to consist solely of a single complete protein structural element and is not designed or selected to consist solely of an entire protein domain; and, in isolation, shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains; and to a chimaeric folded protein domain comprising two or more sequence segments which share common sequences or sequences from common regions in the protein fold of their parent amino acid sequences, wherein the said common region of the fold is not designed or selected to consist solely of one or more complete protein structural elements, and wherein each of said sequence segments: is not designed or selected to consist solely of an entire protein domain; and wherein, in isolation, each said sequence segment shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains.
GB0217404A 2000-02-03 2001-02-02 Combinatorial protein domains Withdrawn GB2375112A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0002492A GB0002492D0 (en) 2000-02-03 2000-02-03 Combinatorial protein domains
US18032600P 2000-02-04 2000-02-04
GB0016346A GB0016346D0 (en) 2000-07-03 2000-07-03 Combinatorial protein domains
GB0019362A GB0019362D0 (en) 2000-08-07 2000-08-07 Combinational protein Domains
PCT/GB2001/000445 WO2001057065A2 (en) 2000-02-03 2001-02-02 Combinatorial protein domains

Publications (2)

Publication Number Publication Date
GB0217404D0 GB0217404D0 (en) 2002-09-04
GB2375112A true GB2375112A (en) 2002-11-06

Family

ID=27447768

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0217404A Withdrawn GB2375112A (en) 2000-02-03 2001-02-02 Combinatorial protein domains

Country Status (7)

Country Link
EP (1) EP1252319A2 (en)
JP (1) JP2003523742A (en)
AU (1) AU3040101A (en)
CA (1) CA2399809A1 (en)
GB (1) GB2375112A (en)
NO (1) NO20023606L (en)
WO (1) WO2001057065A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004239065B2 (en) 2003-05-14 2008-05-15 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
CA2688433A1 (en) 2007-06-06 2008-12-11 Domantis Limited Methods for selecting protease resistant polypeptides
UY31504A1 (en) 2007-11-30 2009-07-17 ANTIGEN UNION CONSTRUCTIONS
MX2011005874A (en) 2008-12-05 2011-06-27 Glaxo Group Ltd Methods for selecting protease resistant polypeptides.
WO2010097385A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
CA2753332A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
US20110305694A1 (en) 2009-02-24 2011-12-15 Paul Andrew Hamblin Multivalent and/or multispecific rankl-binding constructs
EP2853542A1 (en) 2010-11-24 2015-04-01 Glaxo Group Limited Multispecific antigen binding proteins targeting HGF
CA2842099A1 (en) 2011-07-27 2013-01-31 Glaxo Group Limited Antigen binding constructs
EP3561703B1 (en) * 2018-04-25 2021-01-20 Bayer AG Identification of the mating of variable domains from light and heavy chains of antibodies
JPWO2023140360A1 (en) * 2022-01-20 2023-07-27

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021914A1 (en) * 1994-02-10 1995-08-17 Pasteur Merieux Serums Et Vaccins Method for preparing a multicombinatorial library of antibody gene expression vectors
WO1997032017A1 (en) * 1996-02-26 1997-09-04 Morphosys Gesellschaft Für Proteinoptimierung Mbh Novel method for the identification of nucleic acid sequences encoding two or more interacting (poly)peptides
WO1999058655A2 (en) * 1998-05-13 1999-11-18 Diversys Limited Phage display selection system for folded proteins
WO2001042432A2 (en) * 1999-12-08 2001-06-14 Medical Research Council Modified enzymatic activity through subdomain swaps between related alpha/beta-barrel enzymes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021914A1 (en) * 1994-02-10 1995-08-17 Pasteur Merieux Serums Et Vaccins Method for preparing a multicombinatorial library of antibody gene expression vectors
WO1997032017A1 (en) * 1996-02-26 1997-09-04 Morphosys Gesellschaft Für Proteinoptimierung Mbh Novel method for the identification of nucleic acid sequences encoding two or more interacting (poly)peptides
WO1999058655A2 (en) * 1998-05-13 1999-11-18 Diversys Limited Phage display selection system for folded proteins
WO2001042432A2 (en) * 1999-12-08 2001-06-14 Medical Research Council Modified enzymatic activity through subdomain swaps between related alpha/beta-barrel enzymes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Chem. & Biology 1999, 8, pp.R91-R105 - O'Brien et al. *
Gene 2000, 242(2), pp.369-379 - Flajolet et al. *
Nature 1998, 391, pp.288-291 - Crameri et al. *
Nature 2000, 403, pp.617-622 - Altamirano et al. *
Proc. Natl. Acad. Sci. USA 1998, 95, pp.9813-9818 - Hopfner et al. *
Proc. Natl. Acad. Sci. USA 1999, 96, pp.3562-3567 - Ostermeier et al. *
Proc. Natl. Acad. Sci. USA 2000, 97(18), pp.10068-73 - Riechmann et al. *

Also Published As

Publication number Publication date
NO20023606L (en) 2002-10-02
WO2001057065A2 (en) 2001-08-09
GB0217404D0 (en) 2002-09-04
CA2399809A1 (en) 2001-08-09
EP1252319A2 (en) 2002-10-30
NO20023606D0 (en) 2002-07-30
JP2003523742A (en) 2003-08-12
AU3040101A (en) 2001-08-14
WO2001057065A3 (en) 2002-01-31

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Legal Events

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WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)