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GB2295893A - Method and test kits for detection of bacteriophage - Google Patents

Method and test kits for detection of bacteriophage

Info

Publication number
GB2295893A
GB2295893A GB9601733A GB9601733A GB2295893A GB 2295893 A GB2295893 A GB 2295893A GB 9601733 A GB9601733 A GB 9601733A GB 9601733 A GB9601733 A GB 9601733A GB 2295893 A GB2295893 A GB 2295893A
Authority
GB
United Kingdom
Prior art keywords
detection
bacterial
bacteriophage
phage
test kits
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9601733A
Other versions
GB2295893B (en
GB9601733D0 (en
Inventor
Michael Frederick Sanders
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Minister of Agriculture Fisheries and Food UK
Original Assignee
Minister of Agriculture Fisheries and Food UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB939317139A external-priority patent/GB9317139D0/en
Application filed by Minister of Agriculture Fisheries and Food UK filed Critical Minister of Agriculture Fisheries and Food UK
Priority to GB9601733A priority Critical patent/GB2295893B/en
Publication of GB9601733D0 publication Critical patent/GB9601733D0/en
Publication of GB2295893A publication Critical patent/GB2295893A/en
Application granted granted Critical
Publication of GB2295893B publication Critical patent/GB2295893B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for detection, identification and/or quantification of bacteriophage of bacterial host specificity for bacterial genus, species or serotype, based upon the occurrence of release of cell contents, particularly nucleotides e.g. ATP, on lysis of bacterial cell walls on incubation with bacterial host cells. When new phage particles are released at the end of the phage replication cycle nucleotide levels are measured and compared with controls. The method provides for the detection of specific phages which is faster and more sensitive than known techniques. The method is only limited by the availability of host bacteria/target phage pairings.
GB9601733A 1993-08-18 1994-07-29 Method and test kits for detection of bacteriophage Expired - Fee Related GB2295893B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9601733A GB2295893B (en) 1993-08-18 1994-07-29 Method and test kits for detection of bacteriophage

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB939317139A GB9317139D0 (en) 1993-08-18 1993-08-18 Method and test kits for detection of bacteriophage
PCT/GB1994/001649 WO1995005483A1 (en) 1993-08-18 1994-07-29 Method and test kits for detection of bacteriophage
GB9601733A GB2295893B (en) 1993-08-18 1994-07-29 Method and test kits for detection of bacteriophage

Publications (3)

Publication Number Publication Date
GB9601733D0 GB9601733D0 (en) 1996-04-03
GB2295893A true GB2295893A (en) 1996-06-12
GB2295893B GB2295893B (en) 1997-10-08

Family

ID=26303394

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9601733A Expired - Fee Related GB2295893B (en) 1993-08-18 1994-07-29 Method and test kits for detection of bacteriophage

Country Status (1)

Country Link
GB (1) GB2295893B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11667895B2 (en) 2019-05-10 2023-06-06 The Board Of Trustees Of The University Of Alabama Methods and devices related to controlled delivery of phages as a theranostic tool

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2823916A1 (en) * 1977-05-31 1978-12-07 Seppo Kolehmainen METHOD FOR THE SELECTIVE DETERMINATION OF VIBRANT SOMA AND MICROBE CELLS
WO1989005356A1 (en) * 1987-12-03 1989-06-15 The Public Health Laboratory Service Board Assay of salicylates or reduced pyridine nucleotides
WO1992002633A1 (en) * 1990-08-09 1992-02-20 Amersham International Plc Methods for rapid microbial detection
WO1994006931A1 (en) * 1992-09-22 1994-03-31 The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Method and kits for detection of bacteria

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2823916A1 (en) * 1977-05-31 1978-12-07 Seppo Kolehmainen METHOD FOR THE SELECTIVE DETERMINATION OF VIBRANT SOMA AND MICROBE CELLS
WO1989005356A1 (en) * 1987-12-03 1989-06-15 The Public Health Laboratory Service Board Assay of salicylates or reduced pyridine nucleotides
WO1992002633A1 (en) * 1990-08-09 1992-02-20 Amersham International Plc Methods for rapid microbial detection
WO1994006931A1 (en) * 1992-09-22 1994-03-31 The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Method and kits for detection of bacteria

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J.Clin.Microbiol. 1984,20(6),1122-1125 *
J.Food Protection 1984,47(5),388-390 *

Also Published As

Publication number Publication date
GB2295893B (en) 1997-10-08
GB9601733D0 (en) 1996-04-03

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19990729