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GB2289945A - Microbiological test method and reagents - Google Patents

Microbiological test method and reagents

Info

Publication number
GB2289945A
GB2289945A GB9515786A GB9515786A GB2289945A GB 2289945 A GB2289945 A GB 2289945A GB 9515786 A GB9515786 A GB 9515786A GB 9515786 A GB9515786 A GB 9515786A GB 2289945 A GB2289945 A GB 2289945A
Authority
GB
United Kingdom
Prior art keywords
amount
reagents
adp
test method
luciferase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9515786A
Other versions
GB2289945B (en
GB9515786D0 (en
Inventor
David James Squirrell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB939301118A external-priority patent/GB9301118D0/en
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Priority to GB9515786A priority Critical patent/GB2289945B/en
Publication of GB9515786D0 publication Critical patent/GB9515786D0/en
Publication of GB2289945A publication Critical patent/GB2289945A/en
Application granted granted Critical
Publication of GB2289945B publication Critical patent/GB2289945B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for determining the presence and/or amount of microorganisms and/or their intracellular material present in a sample comrpising estimating the amount of adenylate kinase therein by its ability to convert adenosine diphosphate (ADP) to adenosine triphosphate (ATP) and relating that to the presence/or amount of organism and/or intracellular material. The method provides improved sensitivity over existing luciferase/luciferin assays. Reagents including purified ADP and adenyl kinase free luciferase are provided together with test kits including these and apparatus for automated operation of the method. <IMAGE>
GB9515786A 1993-01-21 1995-07-27 Detecting microorganisms by assaying for ATP produced by intracellular adenylate kinase Expired - Fee Related GB2289945B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9515786A GB2289945B (en) 1993-01-21 1995-07-27 Detecting microorganisms by assaying for ATP produced by intracellular adenylate kinase

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB939301118A GB9301118D0 (en) 1993-01-21 1993-01-21 Enzyme linked assays
PCT/GB1994/000118 WO1994017202A1 (en) 1993-01-21 1994-01-21 Microbiological test method and reagents
GB9515786A GB2289945B (en) 1993-01-21 1995-07-27 Detecting microorganisms by assaying for ATP produced by intracellular adenylate kinase

Publications (3)

Publication Number Publication Date
GB9515786D0 GB9515786D0 (en) 1995-10-04
GB2289945A true GB2289945A (en) 1995-12-06
GB2289945B GB2289945B (en) 1997-06-18

Family

ID=26302319

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9515786A Expired - Fee Related GB2289945B (en) 1993-01-21 1995-07-27 Detecting microorganisms by assaying for ATP produced by intracellular adenylate kinase

Country Status (1)

Country Link
GB (1) GB2289945B (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3933592A (en) * 1965-02-17 1976-01-20 Hazleton Laboratories, Incorporated Method of detecting living microorganisms
EP0054676A2 (en) * 1980-12-18 1982-06-30 Roche Diagnostics GmbH Process for determining HL antigens
EP0238352A2 (en) * 1986-03-21 1987-09-23 Minnesota Mining And Manufacturing Company Method for the selective detection of microbial nucleotides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3933592A (en) * 1965-02-17 1976-01-20 Hazleton Laboratories, Incorporated Method of detecting living microorganisms
EP0054676A2 (en) * 1980-12-18 1982-06-30 Roche Diagnostics GmbH Process for determining HL antigens
EP0238352A2 (en) * 1986-03-21 1987-09-23 Minnesota Mining And Manufacturing Company Method for the selective detection of microbial nucleotides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Adv. Biotechnol. (Proc. Int. Ferment. Symp. 6th 1980) Vol. 1, 1981 pages 457-462 *
Chemical Abstracts, Vol. 96, No. 21, 1982, page 350 *
Journal of Biochemical and Biophysical Methods Vol. 1, No. 31979, pages 163-169 *

Also Published As

Publication number Publication date
GB2289945B (en) 1997-06-18
GB9515786D0 (en) 1995-10-04

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20000121