GB2065473A - Process for producing hepatitis b vaccine - Google Patents
Process for producing hepatitis b vaccineInfo
- Publication number
- GB2065473A GB2065473A GB8106701A GB8106701A GB2065473A GB 2065473 A GB2065473 A GB 2065473A GB 8106701 A GB8106701 A GB 8106701A GB 8106701 A GB8106701 A GB 8106701A GB 2065473 A GB2065473 A GB 2065473A
- Authority
- GB
- United Kingdom
- Prior art keywords
- precipitate
- vaccine
- fraction
- hbsag
- particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- SPSXSWRZQFPVTJ-ZQQKUFEYSA-N hepatitis b vaccine Chemical compound C([C@H](NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCSC)C(=O)N[C@@H](CC1N=CN=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)OC(=O)CNC(=O)CNC(=O)[C@H](C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@@H](N)CCCNC(N)=N)C1=CC=CC=C1 SPSXSWRZQFPVTJ-ZQQKUFEYSA-N 0.000 title abstract 2
- 229940124736 hepatitis-B vaccine Drugs 0.000 title abstract 2
- 239000002245 particle Substances 0.000 abstract 4
- 239000002244 precipitate Substances 0.000 abstract 3
- 239000002202 Polyethylene glycol Substances 0.000 abstract 2
- 229920001223 polyethylene glycol Polymers 0.000 abstract 2
- 102000004506 Blood Proteins Human genes 0.000 abstract 1
- 108010017384 Blood Proteins Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 239000007900 aqueous suspension Substances 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- 230000005484 gravity Effects 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 235000004252 protein component Nutrition 0.000 abstract 1
- 238000005199 ultracentrifugation Methods 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
A hepatitis B vaccine is prepared by adjusting the pH of an aqueous suspension of a crude HBsAg to neutral by removing plasma protein components. Polyethylene glycol is added to a 5-6% concentration to thereby remove Dane particles and immune complexes as a precipitate. A further amount of a polyethylene glycol is added to thereby collect HBsAg particles as a precipitate. A specific fraction of HBsAg particles having a particle size of 22 nm and a specific gravity of 1.19-1.21 g/cm<s3>s is recovered from the precipitate by gel filtration and ultracentrifugation. The virus contained in the fraction is inactivated and the fraction is made into a vaccine preparation.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8549379A JPS5610119A (en) | 1979-07-05 | 1979-07-05 | Manufacture of b type hepatitis infection preventive vaccine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB2065473A true GB2065473A (en) | 1981-07-01 |
| GB2065473B GB2065473B (en) | 1984-02-29 |
Family
ID=13860454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB8106701A Expired GB2065473B (en) | 1979-07-05 | 1980-07-03 | Process for producing hepatitis b vaccine |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JPS5610119A (en) |
| GB (1) | GB2065473B (en) |
| WO (1) | WO1981000050A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554157A (en) * | 1981-01-29 | 1985-11-19 | National Research Development Corporation | Hepatitis B vaccine |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZW18282A1 (en) * | 1981-08-31 | 1983-03-23 | Genentech Inc | Preparation of polypeptides in vertebrate cell culture |
| JPS59101426A (en) * | 1982-11-29 | 1984-06-12 | Green Cross Corp:The | Method for producing vaccine for preventing hepatitis B infection |
| FR2560890B1 (en) * | 1984-03-07 | 1987-10-16 | Grp Genie Genetique | COMPOSITION USEFUL FOR THE MANUFACTURE OF VACCINES CONTAINING PARTICLES CARRYING THE SURFACE ANTIGEN OF HEPATITIS B VIRUS AND THE POLYMERIZED HUMAN SERUM ALBUMIN RECEPTOR, ANIMAL CELLS CAPABLE OF PRODUCING SUCH PARTICLES |
| FR2561256B1 (en) * | 1984-03-16 | 1987-01-02 | Pasteur Institut | PROCESS FOR THE PURIFICATION OF BIOLOGICAL PARTICLES OF THE HBS ANTIGEN TYPE, BY ULTRACENTRIFUGATION OF FLOTATION ON DENSITY GRADIENT |
| JPS60197629A (en) * | 1984-03-16 | 1985-10-07 | Chemo Sero Therapeut Res Inst | Method of purification of hbs antigen |
| NL8401066A (en) * | 1984-04-04 | 1985-11-01 | Stichting Centraal Lab | PROCESS FOR PREPARING PREPARATIONS INCREASING HUMORAL AND CELLULAR IMMUNE ACTIVITY |
| JPS60258127A (en) * | 1984-06-04 | 1985-12-20 | Green Cross Corp:The | Manufacturing method for hepatitis B vaccine |
| EP0294071A3 (en) * | 1987-06-03 | 1990-08-01 | Merck & Co. Inc. | Hepatitis b vaccines made with pre-formed aluminum hydroxide gels, and processes thereof |
| EP0384058A1 (en) * | 1989-02-09 | 1990-08-29 | Development Center For Biotechnology | Isolation of hepatitis B surface antigen from transformed yeast cells |
| AU657168B2 (en) * | 1991-09-18 | 1995-03-02 | Amgen, Inc. | Hepatitis B vaccine with bile salt adjuvant |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL286954A (en) * | 1962-01-03 | |||
| BR6915288D0 (en) * | 1969-03-15 | 1973-02-08 | Bayer Ag | PROCESS FOR THE PURIFICATION OF SOLUTIONS OF THE FOOT-AND-Mouth Disease VIRUS |
| US3989818A (en) * | 1970-08-14 | 1976-11-02 | South African Inventions Development Corporation | Influenza virus vaccine |
| GB1356413A (en) * | 1971-09-09 | 1974-06-12 | Pfizer Ltd | Method of obtaining purified australia antigen from blood serum |
| US3951937A (en) * | 1973-12-20 | 1976-04-20 | The Community Blood Council Of Greater New York, Inc. | Large scale purification of hepatitis type B antigen using polyethylene glycol |
| US3994870A (en) * | 1974-01-31 | 1976-11-30 | The Community Blood Council Of Greater New York, Inc. | Purification of hepatitis B surface antigen |
| FR2303562A1 (en) * | 1975-03-14 | 1976-10-08 | Community Blood Council Greate | NEW ANTIGEN PREPARATION FOR USE AS A VACCINE |
| SE437329B (en) * | 1975-03-14 | 1985-02-25 | Community Blood Council | PUT OUT OF BLOOD SERUM TO PROVIDE VACCIN AGAINST VIRUS Hepatitis |
| FR2336141A1 (en) * | 1975-12-23 | 1977-07-22 | Trepo Christian | NEW MEDICINE TO TREAT ACUTE OR CHRONIC HEPATITIS B VIRUS INFECTIONS |
| DE2750045A1 (en) * | 1977-11-09 | 1979-05-10 | Behringwerke Ag | METHOD FOR REMOVING DETERGENTS FROM VIRUS ANTIGENS SUSPENSIONS |
-
1979
- 1979-07-05 JP JP8549379A patent/JPS5610119A/en active Granted
-
1980
- 1980-07-03 WO PCT/US1980/000834 patent/WO1981000050A1/en active Application Filing
- 1980-07-03 GB GB8106701A patent/GB2065473B/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554157A (en) * | 1981-01-29 | 1985-11-19 | National Research Development Corporation | Hepatitis B vaccine |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1981000050A1 (en) | 1981-01-22 |
| JPS5721246B2 (en) | 1982-05-06 |
| JPS5610119A (en) | 1981-02-02 |
| GB2065473B (en) | 1984-02-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |