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GB2019563A - Assay of Gram-negative bacteria - Google Patents

Assay of Gram-negative bacteria

Info

Publication number
GB2019563A
GB2019563A GB7911610A GB7911610A GB2019563A GB 2019563 A GB2019563 A GB 2019563A GB 7911610 A GB7911610 A GB 7911610A GB 7911610 A GB7911610 A GB 7911610A GB 2019563 A GB2019563 A GB 2019563A
Authority
GB
United Kingdom
Prior art keywords
gram
assay
negative bacteria
microorganisms
sedimenting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7911610A
Other versions
GB2019563B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of GB2019563A publication Critical patent/GB2019563A/en
Application granted granted Critical
Publication of GB2019563B publication Critical patent/GB2019563B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A procedure and kit for assay of Gram-negative bacteria in biological fluids by lysing cells in the fluid at pH 6 to 8, removing cellular debris, sedimenting microorganisms from the liquid phase at 5,000 G or more, incubating the microorganisms with Limulus polyphemus amebocyte lysate in aqueous medium, and measuring the amount of resultant turbidity.
GB7911610A 1978-04-20 1979-04-03 Assay of gram-negative bacteria Expired GB2019563B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US89829178A 1978-04-20 1978-04-20

Publications (2)

Publication Number Publication Date
GB2019563A true GB2019563A (en) 1979-10-31
GB2019563B GB2019563B (en) 1982-07-28

Family

ID=25409227

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7911610A Expired GB2019563B (en) 1978-04-20 1979-04-03 Assay of gram-negative bacteria

Country Status (9)

Country Link
JP (1) JPS54149699A (en)
BE (1) BE875716A (en)
CA (1) CA1114271A (en)
CH (1) CH641493A5 (en)
DE (1) DE2915569A1 (en)
FR (1) FR2423784A1 (en)
GB (1) GB2019563B (en)
IT (1) IT1119718B (en)
NL (1) NL7902983A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4322217A (en) 1980-06-27 1982-03-30 Mallinckrodt, Inc. Process for preparing Limulus lysate
US5594113A (en) * 1988-06-23 1997-01-14 Associates Of Cape Cod, Inc. Endotoxin binding and neutralizing protein and uses thereof
EP2209904A2 (en) * 2007-10-10 2010-07-28 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in urine
GB2477752A (en) * 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
US8519358B2 (en) 2008-02-05 2013-08-27 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
US9862920B2 (en) 2012-12-11 2018-01-09 Pocared Diagnostics Ltd. Optics cup with curved bottom
US10288632B2 (en) 2009-09-21 2019-05-14 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4322217A (en) 1980-06-27 1982-03-30 Mallinckrodt, Inc. Process for preparing Limulus lysate
US5594113A (en) * 1988-06-23 1997-01-14 Associates Of Cape Cod, Inc. Endotoxin binding and neutralizing protein and uses thereof
US5614369A (en) * 1988-06-23 1997-03-25 Associates Of Cape Cod, Inc. Endotoxin binding and neutralizing protein and uses thereof
US5627266A (en) * 1988-06-23 1997-05-06 Associates Of Cape Cod, Inc. Endotoxin binding and neutralizing protein and uses thereof
US5747455A (en) * 1988-06-23 1998-05-05 Associates Of Cape Cod, Inc. Endotoxin binding and neutralizing protein and uses thereof
US6222021B1 (en) * 1988-06-23 2001-04-24 Associates Of Cape Cod, Inc. Fragments of endotoxin binding protein and uses thereof
US6384200B1 (en) 1988-06-23 2002-05-07 Associates Of Cape Cod, Inc. Endotoxin binding and neutralizing protein and uses thereof
EP2209904A4 (en) * 2007-10-10 2013-05-29 Pocared Diagnostics Ltd System for conducting the identification of bacteria in urine
US8808649B2 (en) 2007-10-10 2014-08-19 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in urine
US10656140B2 (en) 2007-10-10 2020-05-19 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in urine
EP2209904A2 (en) * 2007-10-10 2010-07-28 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in urine
US9606105B2 (en) 2007-10-10 2017-03-28 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in urine
US8519358B2 (en) 2008-02-05 2013-08-27 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
US9506866B2 (en) 2008-02-05 2016-11-29 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
US10073036B2 (en) 2008-02-05 2018-09-11 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
US10801962B2 (en) 2008-02-05 2020-10-13 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
US10288632B2 (en) 2009-09-21 2019-05-14 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
US11002752B2 (en) 2009-09-21 2021-05-11 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
GB2477752A (en) * 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
WO2011098820A1 (en) * 2010-02-11 2011-08-18 Arab Biotechnology Company Phage-based limulus amoebocyte lysate assay for the rapid detection of bacteria
US9862920B2 (en) 2012-12-11 2018-01-09 Pocared Diagnostics Ltd. Optics cup with curved bottom
US10731123B2 (en) 2012-12-11 2020-08-04 Pocared Diagnostics Ltd. Optics cup with curved bottom

Also Published As

Publication number Publication date
CH641493A5 (en) 1984-02-29
CA1114271A (en) 1981-12-15
FR2423784B3 (en) 1982-02-26
GB2019563B (en) 1982-07-28
IT1119718B (en) 1986-03-10
NL7902983A (en) 1979-10-23
IT7921820A0 (en) 1979-04-12
DE2915569A1 (en) 1979-11-08
FR2423784A1 (en) 1979-11-16
JPS54149699A (en) 1979-11-24
BE875716A (en) 1979-08-16

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee